Shenshenmycin soluble liquid and application thereof

Abstract

The invention discloses a phenazino-1-carboxylic acid soluble liquid and application thereof. The phenazino-1-carboxylic acid soluble liquid is prepared from the following raw materials in percentage by weight: 0.1-10% of phenazino-1-carboxylic acid, 0.1-10% of a solvent, 2-20% of alkali, 1-20% of a surfactant, 0.1-10% of an anti-freezing agent and the balance of deionized water. The soluble liquid of the phenazino-1-carboxylic acid has more excellent solubility and dispersibility, improves the absorption efficiency of plants, promotes the pesticide effect to be fully exerted, and is superior to a phenazino-1-carboxylic acid suspending agent in the effect of preventing and treating plant diseases.

Description

Technical Field

[0001] This invention relates to the field of biopesticide technology, and in particular to a soluble liquid formulation of shenqinmycin and its application. Background Technology

[0002] Shenqinmycin is a novel microbial-derived green pesticide developed using the metabolites of plant rhizosphere-promoting Pseudomonas. Its chemical name is phenazine-1-carboxylic acid (PCA). It can effectively control a variety of plant diseases, including wheat scab and take-all, rice sheath blight, rice false smut, and rice blast, cucumber downy mildew and gray mold, watermelon wilt, and pepper blight, and also promotes plant growth.

[0003] The main research and development directions for pesticide formulations are aqueous solutions, high-content formulations, solid formulations, and multifunctional formulations. Shenqinmycin has poor water solubility, and currently, the market mainly offers single-formulation suspensions. Suspensions often require vigorous shaking, which can lead to uneven administration and poor dispersibility. Soluble liquid formulations, on the other hand, have good uniformity and superior solubility and dispersibility, making them easier to store and transport, and thus improving plant absorption efficiency and maximizing efficacy. This is of great significance for the development of new pesticide formulations, the promotion of biopesticides, and ensuring the quality and safety of agricultural products. Summary of the Invention

[0004] To overcome the problems existing in the related technologies, this application provides a soluble liquid form of Shenqinmycin and its application. The soluble liquid form of Shenqinmycin has better solubility and dispersibility, improves the absorption efficiency of plants and promotes the full exertion of the drug effect, and is superior to Shenqinmycin suspension in the prevention and control of plant diseases.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution: A soluble liquid preparation of shensomycin, characterized in that it is made from the following raw materials in weight percentages: 0.1%-10% shensomycin, 0.1%-10% solvent, 2%-20% alkali, 1%-20% surfactant, 0.1%-10% antifreeze, with the balance made up to 100% with deionized water.

[0006] The aforementioned shensomycin soluble liquid is made from the following raw materials in weight percentage: shensomycin 1%-10%, solvent 1%-10%, alkali 5%-20%, surfactant 1%-20%, antifreeze 1%-10%, with the balance made up to 100% with deionized water.

[0007] The solvent is ethanol.

[0008] The alkali is one of potassium salt or ethanolamine salt.

[0009] The surfactant is any one or a mixture of calcium dodecylbenzenesulfonate, sodium dodecyl sulfate, calcium lignin sulfonate, sodium fatty alcohol polyoxyethylene ether sulfate, fatty alcohol polyoxyethylene ether phosphate, sorbitol fatty acid ester, natriuretic polyoxyethylene ether, and sodium sarcosinate.

[0010] The antifreeze is one or more combinations of ethanol, ethylene glycol, isopropanol, sodium chloride, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium carbonate, and sodium hydroxide.

[0011] Application of a soluble liquid formulation of shenqinmycin in the preparation of agents for the prevention and control of fungal diseases in plants.

[0012] The plant fungal diseases mentioned include wheat scab, rice sheath blight, pepper white mold, cucumber gray mold, rice blast, peanut white mold, plant wilt, and corn anthracnose.

[0013] Application of a soluble liquid form of shenqinmycin in the preparation of a growth-promoting agent for watermelon seedlings.

[0014] The soluble liquid form of Shensomycin has a broad-spectrum antibacterial effect, specifically targeting *Rhizoctonia solani*, *Botrytis cinerea*, *Sclerotinia sclerotiorum*, *Fusarium graminearum*, *Fusarium oxysporum*, *Bacillus oryzae*, *Anthracnose*, *Rhizoctonia solani*, and *Alternaria alternata*.

[0015] According to embodiments of the present invention, the antibacterial effect of the shensomycin soluble liquid is superior to that of the shenqinmycin suspension, specifically including gray mold, sclerotinia sclerotiorum, wheat scab, Fusarium, Fusarium oxysporum, rice blast fungus, anthracnose fungus, Rhizoctonia solani, and Alternaria alternata.

[0016] According to embodiments of the present invention, the serosol soluble liquid can inhibit the growth of a variety of pathogens, thus enabling the prevention and control of a variety of plant diseases.

[0017] The technical solution provided in this application may include the following beneficial effects: This shensomycin soluble liquid formulation and its application: As a soluble liquid, shensomycin technical material, which is poorly soluble in water, is formulated into a soluble liquid. When used, it is diluted with water and exists in ionic form in the solution, giving the drug components strong penetrability and permeability, superior solubility and dispersibility, and more complete efficacy, thus improving the utilization rate of the drug. It is of great significance for the development of new pesticide formulations and the wider application of biological pesticides.

[0018] This seromonadine soluble solution and its application: The seromonadine soluble solution has a broad-spectrum antibacterial effect, effectively inhibiting the growth and reproduction of pathogenic fungi, thus effectively controlling plant diseases. It also has growth-promoting functions, controlling diseases and promoting plant growth in an environmentally friendly way, and has broad application prospects. Compared with seromonadine suspension, it has better control and growth-promoting effects, thereby reducing the dosage of seromonadine and lowering costs. Attached Figure Description

[0019] The above and other objects, features and advantages of this application will become more apparent from the more detailed description of exemplary embodiments thereof in conjunction with the accompanying drawings, wherein the same reference numerals generally represent the same components in the exemplary embodiments thereof.

[0020] Figure 1 This is a schematic diagram illustrating the application of shenqinmycin soluble liquid in the control of white mold disease in peppers, as shown in the embodiments of this application; Figure 2 This is a schematic diagram illustrating the application of shenqinmycin soluble liquid in the control of cucumber gray mold, as shown in the embodiments of this application; Figure 3 This is a schematic diagram illustrating the application of shenqinmycin soluble liquid in the control of wheat scab, as shown in the embodiments of this application. Figure 4 This is a schematic diagram illustrating the application of shenqinmycin soluble liquid in promoting watermelon seedling growth, as shown in the embodiments of this application. Detailed Implementation

[0021] The technical solution and technical effects of the present invention will be further described below with reference to specific embodiments. It should be noted that the described embodiments are only intended to facilitate the understanding of the present invention and do not limit it in any way. Example 1

[0022] The soluble liquid of seromonadine contains the following weight percentages of its components: Shenqinmycin 0.5%, solvent 1%, alkali 8%, surfactant 12%, antifreeze 7%, and the balance is made up to 100% with deionized water. Example 2

[0023] The soluble liquid of seromonadine contains the following weight percentages of its components: Shenqinmycin 6%, solvent 7%, alkali 15%, surfactant 3%, antifreeze 2%, and the balance is made up to 100% with deionized water. Example 3

[0024] The antibacterial effect of serosol soluble liquid Indoor Antibacterial Effect Determination Method for Fungal Diseases: The mycelial growth rate method was used, and the experimental method referred to "People's Republic of China Agricultural Industry Standard NY / T1156.2-2006". First, 5-7 different concentration gradients were set up for single agents and various mixtures. A water control group was included, and the experiment was repeated three times. Using the mycelial growth rate method, the cultured pathogen was aseptically cut into mycelial cakes from the edge of the colony using an 8mm diameter punch. The mycelial cakes were then inoculated into the center of culture plates of different agent concentrations using an inoculation needle, with the mycelial side facing upwards. The plates were covered and placed in an oven at a suitable temperature for cultivation. When the control group colonies grew to approximately two-thirds of their original size, the colony diameter was measured with calipers in millimeters (mm). The diameter of each colony was measured vertically once using the cross-sectional method, and the average value was taken. The mycelial growth inhibition rate of each agent treatment was calculated based on the measurement results. Regression analysis was performed based on the logarithmic value of each agent concentration and the corresponding probability value of mycelial growth inhibition rate. The toxicity regression equation and effective median concentration EC50 of the corresponding agent were obtained using the professional statistical function of DPS data software for bioassay.

[0025] The pathogen cultures included *Rhizoctonia solani*, *Botrytis cinerea*, *Sclerotinia sclerotiorum*, *Fusarium graminearum*, *Fusarium oxysporum*, *Bacillus oryzae*, *Anthracnose*, *Rhizoctonia solani*, and *Alternaria alternata*.

[0026] The culture medium used in the experiment was: PDA solid culture medium. The results of the antifungal effect test of shenqinmycin soluble liquid against pathogenic fungi are shown in Table 1. Table 1. EC50 of shenqinmycin soluble liquid against pathogenic fungi.

[0027] As shown in Table 1, shenqinmycin soluble liquid has a broad-spectrum antifungal effect against pathogenic fungi. Among them, except for the antifungal effect against *Rhizoctonia solani*, the antifungal effects of shenqinmycin potassium salt and shenqinmycin ethanolamine salt against pathogenic fungi are better than those of shenqinmycin soluble liquid. This indicates that shenqinmycin soluble liquid is superior to shenqinmycin suspension in its antifungal effect against pathogens.

[0028] Example 4: Application of Shenqinmycin Soluble Liquid in the Control of White Mold Disease in Peppers Different concentrations of the reagents were prepared by mixing shenzimidic acid, shenzimidic acid potassium salt, and shenzimidic acid ethanolamine salt with water, each at a concentration of 40 ppm. Pepper leaves aged 30-50 days were collected, wrapped in cotton, and placed on disposable petri dishes containing two filter paper discs. Each disc was kept moist with 3 ml of water. Water was used as a control. The reagents were sprayed on each leaf, and after 24 hours, *Sclerotinia sclerotiorum* (a causal agent for sclerotia) was inoculated (the pathogen was cultured for 3 days), with holes punched at the very edge. Four to five wounds were made on each pepper leaf, and the pathogen was inoculated into the wounds. The leaves were then cultured at 26 ℃ under moist conditions. After 2 days, the disease development was observed and photographed. Image-Pro Plus software was used to measure the length, area, and width of leaf lesions, and SPSS was used for data analysis. Each replicate consisted of four leaves, with two pathogens inoculated on each leaf.

[0029] The results of the test on the control effect of shenqinmycin soluble liquid on white mold disease of pepper are shown in Table 2. Table 2. In vitro control effects of different agents on white fungus on pepper leaves

[0030] From Table 2 Figure 1 It can be seen that the soluble liquid form of Shenqinmycin has a significant in vitro control effect on white mold disease of pepper. Among them, the potassium salt and ethanolamine salt of Shenqinmycin have significantly smaller lesion areas than Shenqinmycin, and their control effect is better than that of Shenqinmycin. This indicates that the soluble liquid form of Shenqinmycin is superior to the suspension form of Shenqinmycin in the in vitro control effect on white mold disease of pepper.

[0031] Example 5: Application of Shenqinmycin Soluble Liquid in the Control of Gray Mold in Cucumber Different concentrations of the reagents were prepared by mixing shenqinmycin, shenqin potassium salt, and shenqin ethanolamine salt with water, all at a concentration of 40 ppm. Cucumber leaves that had grown for approximately 20-30 days were picked, wrapped in cotton, and placed on disposable petri dishes containing two filter paper discs. Each disc was kept moist with 3 ml of water. Water was used as a control. The reagents were sprayed on each leaf, and the pathogen (cultured for 3 days) was inoculated 24 hours later, with holes punched at the very edge. Four to five wounds were made on each cucumber leaf, and the pathogen was inoculated into the wounds. The leaves were then cultured at 26 ℃ under moist conditions. After 2-3 days, the disease development was observed, and photographs were taken. The length, area, and width of the leaf lesions were measured using Image-Pro Plus software.

[0032] Table 3. In vitro control effects of different agents on gray mold on cucumber leaves

[0033] From Table 3 Figure 2It can be seen that the soluble liquid form of Shenqinmycin has a significant in vitro control effect on gray mold of cucumber. Among them, the potassium salt and ethanolamine salt of Shenqinmycin are significantly lower than Shenqinmycin in terms of lesion area, lesion length and lesion width, and their control effect is better than that of Shenqinmycin. This indicates that the soluble liquid form of Shenqinmycin is better than the suspension form of Shenqinmycin in terms of in vitro control effect on gray mold of cucumber.

[0034] Example 6: Application of Shenqinmycin Soluble Liquid in the Control of Wheat Fusarium Head Blight Different concentrations of the fungicide were prepared by mixing shenzimidic acid, shenzimidic acid potassium salt, and shenzimidic acid ethanolamine salt with water, each at a concentration of 40 ppm. The corresponding fungicide was evenly sprayed onto the surface of wheat plants. 24 hours later, wheat scab pathogen blocks were inoculated. The fungus was inoculated onto wheat leaves, selecting 10 leaves per pot, making 4-5 wounds on each leaf, and then placing the fungal cake at the wounds. The leaves were then covered with plastic wrap to retain moisture. The disease development was observed for 3-7 days. The leaves were cut off and photographed, and the length, area, and width of the leaf lesions were measured using Image-Pro Plus software.

[0035] Table 4. Experimental results on the control efficacy of different agents against Fusarium head blight of wheat in pots.

[0036] From Table 4 Figure 3 It can be seen that Shenqinmycin soluble liquid has a significant control effect on wheat scab. Among them, Shenqinmycin potassium salt and Shenqinmycin ethanolamine salt have significantly lower lesion area and lesion length than Shenqinmycin, and their control effect is better than that of Shenqinmycin. This indicates that Shenqinmycin soluble liquid is superior to Shenqinmycin suspension in the control of wheat scab.

[0037] Example 7: Application of Shenqinmycin Soluble Liquid in Promoting Watermelon Seedling Growth Different concentrations of the agents were prepared by mixing shenqinmycin, shenqinmycin potassium salt, and shenqinmycin ethanolamine salt with water, each at a concentration of 100 ppm. Watermelon seedlings with uniform growth were transplanted when they had two leaves and one bud. Three days later, shenqinmycin suspension, shenqinmycin potassium salt, and shenqinmycin ethanolamine salt were diluted and applied to the roots at a rate of 50 mL / plant. Initial plant height was measured. A second application of shenqinmycin was performed at a rate of 50 mL / plant. One week later, plant height was measured and growth was observed. Once a significant growth-promoting effect was observed, the plants were harvested, and plant height, root length, above-ground / below-ground fresh / dry weight, and chlorophyll content were measured. Table 5 Effects of different reagents on watermelon seedling growth

[0038] From the above Figure 4Table 5 shows that both shenqinmycin suspension and shenqinmycin soluble liquid drenching had significant growth-promoting effects on potted watermelons, with significantly higher average plant height and average above-ground fresh weight compared to the water control. Compared to shenqinmycin suspension, shenqinmycin ethanolamine resulted in a higher average plant height increase; while shenqinmycin potassium salt resulted in a lower average plant height increase, but a higher average above-ground fresh weight. There were no significant differences in chlorophyll content among the treatments, but all were higher than the water control, with shenqinmycin ethanolamine salt showing the highest content.

[0039] The solution of this application has been described in detail above with reference to the accompanying drawings. In the above embodiments, the descriptions of each embodiment have different emphases; for parts not described in detail in a certain embodiment, please refer to the relevant descriptions of other embodiments. Those skilled in the art should also understand that the actions and modules involved in the specification are not necessarily essential to this application. Furthermore, it is understood that the steps in the method of this application's embodiments can be adjusted, combined, and deleted according to actual needs, and the modules in the device of this application's embodiments can be combined, divided, and deleted according to actual needs.

[0040] The various embodiments of this application have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A soluble liquid preparation of seromonadine, characterized in that, It is made from the following raw materials in weight percentage: 0.1%-10% shenqinmycin, 0.1%-10% solvent, 2%-20% alkali, 1%-20% surfactant, 0.1%-10% antifreeze, and the balance is made up to 100% with deionized water.

2. The serotonin soluble liquid according to claim 1, characterized in that, It is made from the following raw materials by weight percentage: 1%-10% shenqinmycin, 1%-10% solvent, 5%-20% alkali, 1%-20% surfactant, 1%-10% antifreeze, and the balance is made up to 100% with deionized water.

3. The serosol-soluble liquid formulation according to claim 1 or 2, characterized in that, The solvent is ethanol.

4. The serosol-soluble liquid formulation according to claim 1 or 2, characterized in that, The alkali is one of potassium salt or ethanolamine salt.

5. The serosol-soluble liquid formulation according to claim 1 or 2, characterized in that, The surfactant is any one or a mixture of calcium dodecylbenzenesulfonate, sodium dodecyl sulfate, calcium lignin sulfonate, sodium fatty alcohol polyoxyethylene ether sulfate, fatty alcohol polyoxyethylene ether phosphate, sorbitol fatty acid ester, natriuretic polyoxyethylene ether, and sodium sarcosinate.

6. The serosol-soluble liquid formulation according to claim 1 or 2, characterized in that, The antifreeze is one or more combinations of ethanol, ethylene glycol, isopropanol, sodium chloride, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium carbonate, and sodium hydroxide.

7. Application of a soluble liquid formulation of shenqinmycin in the preparation of agents for the prevention and control of fungal diseases in plants.

8. The application of the shenqinmycin soluble liquid according to claim 7 in the preparation of agents for controlling plant fungal diseases, characterized in that, The plant fungal diseases mentioned include wheat scab, rice sheath blight, pepper white mold, cucumber gray mold, rice blast, peanut white mold, plant wilt, and corn anthracnose.

9. Application of a soluble liquid form of shenqinmycin in the preparation of a growth-promoting agent for watermelon seedlings.

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