Dendrobium big-leaf tea composition for assisting in reducing blood sugar, nourishing yin and moistening dryness and preparation method of dendrobium big-leaf tea composition

Abstract

The invention provides a dendrobium big-leaf tea composition for assisting in reducing blood sugar, nourishing yin and moistening dryness and a preparation method thereof, the composition comprises the following core raw materials in parts by weight: 8-32 parts of dendrobium and 25-65 parts of white tea, and can also be supplemented with a yin nourishing synergistic component and a blood sugar reducing auxiliary component; wherein the dendrobium is subjected to liquid nitrogen cryogenic embrittlement-low-temperature airflow wall breaking treatment, the white tea is subjected to endogenous enzyme directional low-temperature anaerobic conversion treatment, and the finished product is subjected to pulsed light low-temperature sterilization. The composition disclosed by the invention is scientific in compatibility, has the dual effects of assisting in reducing blood sugar, nourishing yin and moistening dryness due to synergistic interaction of the dendrobium and the white tea, is high in retention rate and dissolution rate of active ingredients, mild and non-irritant in taste, high in safety and suitable for long-term drinking of people with relatively high blood sugar and yin deficiency and dryness-heat constitution.

Description

Technical Field

[0001] This invention relates to the field of functional food technology, and in particular to a Dendrobium officinale leaf tea composition for assisting in lowering blood sugar and nourishing yin and moisturizing dryness, and its preparation method. Background Technology

[0002] In recent years, with changes in residents' dietary structure and lifestyle, the incidence of type 2 diabetes has been increasing year by year and is showing a trend towards affecting younger people. In Traditional Chinese Medicine (TCM), diabetes falls under the category of "Xiao Ke Bing" (wasting and thirsting disease), with its core pathogenesis being "Yin deficiency as the root cause and dryness and heat as the manifestation." Yin deficiency and dryness / heat persist throughout the entire course of the disease, clinically manifesting as symptoms such as excessive thirst, dry mouth and throat, increased appetite and hunger, night sweats, and weight loss. Therefore, developing functional tea drinks that combine the effects of assisting in lowering blood sugar and nourishing Yin and moisturizing dryness, are of medicinal and edible origin, are safe and mild, and suitable for long-term consumption, has significant market value and social significance.

[0003] Dendrobium officinale and white tea are medicinal and edible ingredients with naturally complementary effects. The former can nourish yin and promote body fluid production, protect pancreatic function, and improve insulin resistance, while the latter can clear heat and moisten dryness, inhibit α-glucosidase, and stabilize postprandial blood sugar. However, existing compound tea drinks still have many technical defects: First, the compatibility lacks scientific basis, and most are simply additives without forming a rigorous compatibility system targeting the core pathogenesis of diabetes, thus failing to achieve the synergistic effect of nourishing yin and lowering blood sugar. Some products have redundant ingredients, which can easily increase the metabolic burden on the body. Second, the pretreatment process of raw materials is insufficient. Conventional processing methods for Dendrobium officinale cannot simultaneously achieve cell wall disruption rate and preservation of heat-sensitive active ingredients. Conventional white tea processing suffers from insufficient enrichment of active ingredients, a high proportion of stimulating components such as ester-type catechins and caffeine, and strong cold properties, making it unsuitable for long-term consumption by target groups with weak spleen and stomach. Thirdly, sterilization processes cannot simultaneously ensure sterilization effectiveness and activity protection. High-temperature sterilization can easily cause degradation of active ingredients and deterioration of flavor, while irradiation sterilization carries the risk of residues. Fourthly, the product has poor batch-to-batch stability, and its efficacy is out of sync with clinical needs, making it difficult to achieve stable industrial production. Summary of the Invention

[0004] In view of this, the present invention proposes a Dendrobium officinale large-leaf tea composition for assisting in lowering blood sugar and nourishing yin and moisturizing dryness, and its preparation method, thereby solving the above problems.

[0005] The technical solution of the present invention is as follows: a Dendrobium officinale large-leaf tea composition for assisting in lowering blood sugar and nourishing yin and moisturizing dryness, comprising, by weight, the core raw materials: 8-32 parts of Dendrobium officinale and 25-65 parts of white tea.

[0006] Furthermore, the composition, by weight, includes the following core ingredients: 10-28 parts of Dendrobium and 35-45 parts of white tea.

[0007] Furthermore, the Dendrobium is Dendrobium officinale, specifically fine powder that has undergone liquid nitrogen cryogenic embrittlement-low temperature airflow cell disruption treatment, with a fine powder particle size D90≤5μm and a cell disruption rate ≥98%.

[0008] Further, the specific steps for the fine powder obtained by liquid nitrogen cryogenic embrittlement-low temperature airflow cell disruption are as follows: Take the stems of Dendrobium officinale, wash them, dry them with low temperature hot air at 40-42℃ until the moisture content is ≤6%, and cut them into 3-5mm segments; place the Dendrobium segments in a liquid nitrogen cryogenic tank and cryogenically embrittle them at -20~-15℃ for 15-25 minutes; after embrittlement, immediately send them into a low temperature airflow cell disruptor and disrupt them at -12~-8℃, feed pressure 0.6~0.8MPa, and crushing pressure 0.8-1.0MPa to obtain cell-wall broken Dendrobium fine powder, which is then passed through a 200-400 mesh sieve for later use.

[0009] Furthermore, the white tea is a highly active white tea made from fresh organic tea leaves from the Baisha meteorite crater in Hainan, consisting of one bud and two leaves, through a low-temperature anaerobic transformation process using endogenous enzymes.

[0010] Further, the specific steps of the endogenous enzyme-directed low-temperature anaerobic transformation treatment are as follows: Take fresh white tea leaves and wither them naturally indoors for 54-66 hours until the moisture content of the fresh leaves is 38-42%; place the withered tea leaves in a sealed anaerobic chamber and, under the conditions of temperature 21-24℃ and relative humidity 62-68%, anaerobically activate endogenous polyphenol oxidase and glycosidase for directed transformation for 24-36 hours; after the transformation is completed, dry the tea leaves with low-temperature hot air at 36-39℃ until the moisture content is ≤5.5%, and then pulverize them to 30-60 mesh using a low-temperature airflow pulverizer to obtain white tea powder for later use.

[0011] Furthermore, it also includes 2-8 parts of Polygonatum odoratum, 1-4 parts of lily bulb, 1-3 parts of Ophiopogon japonicus, 2-6 parts of mulberry leaf, 1-4 parts of kudzu root, and 1-2 parts of bitter melon peptide powder.

[0012] Preferably, the bitter melon peptide powder is prepared by a method comprising the following steps: S01. Enzymatic hydrolysis: Disperse bitter melon powder in buffer solution, add compound protease, and enzymatically hydrolyze at 50-55℃ for 3-5 hours. The compound protease is composed of alkaline protease and flavor protease at an activity unit ratio of 2:1. S02, Purification: After inactivating the enzymatic hydrolysate, it is sequentially separated by an ultrafiltration membrane with a molecular weight cutoff of 10 kDa and a nanofiltration membrane with a molecular weight cutoff of 1 kDa, and the nanofiltration retentate is collected. S03. Drying: The refined peptide liquid is concentrated and spray-dried at an inlet air temperature of 150°C and an outlet air temperature of 80°C to obtain the bitter melon peptide powder.

[0013] Furthermore, the preparation method of the Dendrobium officinale large-leaf tea composition includes the following steps: S1. Pretreatment: Take Solomon's seal, lily bulb, ophiopogon japonicus, mulberry leaf, and kudzu root, remove impurities, wash them, dry them at a low temperature of 40~45℃ with hot air until the moisture content is ≤7%, pulverize them to 40~60 mesh, sieve to remove impurities, and set them aside for use. S2. Homogeneous mixing: Dendrobium fine powder, white tea powder, bitter melon peptide powder and the powder obtained in S1 are placed into a three-dimensional motion mixer and mixed for 30-60 minutes at 15-25℃ and relative humidity ≤40% until the mixture is uniform to obtain the composite mixture base material. S3. Low-temperature sterilization and molding: The mixed base material is sterilized at a low temperature without heat. The moisture content of the material after sterilization is controlled to be ≤7.0%, the total number of colonies is ≤1000 CFU / g, and the total number of molds and yeasts is ≤100 CFU / g. Then, it is quantitatively packaged according to the target tea beverage dosage form to obtain the Dendrobium large-leaf tea composition.

[0014] Furthermore, the non-thermal low-temperature sterilization described in step S3 is pulsed high-intensity light sterilization, with a pulse width of 250-350 μs, a pulse frequency of 3-5 Hz, and a single pulse energy of 150-250 J. A xenon lamp in double-sided flash mode is used, with a vertical distance of 8-12 cm from the material surface. The material layer thickness is 2-3 mm, and each side is flashed 8-15 times for 2-4 minutes each time. Temperature is controlled throughout the sterilization process using cold air circulation, ensuring the material temperature remains ≤40℃ and the relative humidity of the sterilization environment is ≤35%. The above-mentioned Dendrobium officinale leaf tea composition can be used to prepare tea drinks, solid beverages or related health foods that help lower blood sugar and improve yin deficiency and dryness syndrome.

[0015] Compared with the prior art, the beneficial effects of the present invention are: This invention uses Dendrobium officinale and white tea as its core active ingredients, precisely combining them to address the core pathogenesis of diabetes mellitus characterized by "yin deficiency as the root cause and dryness and heat as the manifestation." From a Traditional Chinese Medicine perspective, Dendrobium officinale primarily nourishes the yin of the kidneys and stomach, replenishing the root cause of yin deficiency, while white tea primarily clears heat from the lungs and stomach, quickly alleviating the manifestation of dryness and heat. The combination nourishes yin without being cloying, and clears heat without damaging yin, comprehensively covering the upper, middle, and lower jiao (upper, middle, and lower burners) of yin deficiency and dryness-heat syndrome, synergistically enhancing the effects of nourishing yin, moistening dryness, and quenching thirst. From a modern pharmacological perspective, Dendrobium officinale regulates basal glucose metabolism and fasting blood glucose by protecting pancreatic β-cells and improving insulin resistance, while white tea stabilizes postprandial blood glucose by effectively inhibiting α-glucosidase activity. The complementary targets of these two ingredients achieve all-day blood glucose control, synergistically enhancing the auxiliary effect of lowering blood sugar. The addition of yin-nourishing synergistic components and blood sugar-lowering auxiliary components further strengthens the core efficacy.

[0016] The entire formula uses Dendrobium officinale as the principal ingredient to nourish Yin and address the root cause, as well as regulate basal glucose metabolism; white tea as the secondary ingredient to clear heat and address the symptoms, and control postprandial blood sugar; Polygonatum odoratum, lily bulb, and Ophiopogon japonicus as auxiliary ingredients for nourishing Yin, and mulberry leaf, kudzu root, and bitter melon peptide powder as auxiliary ingredients for lowering blood sugar, to strengthen the core effects of the principal and secondary ingredients and to make up for the shortcomings in symptoms and targets; the entire formula has no redundant auxiliary ingredients, and all raw materials are focused on the two core effects of assisting in lowering blood sugar and nourishing Yin and moisturizing dryness.

[0017] Dendrobium officinale undergoes a liquid nitrogen cryogenic embrittlement-low-temperature airflow cell-wall breaking process, avoiding the degradation and inactivation of heat-sensitive active ingredients such as Dendrobium polysaccharides and alkaloids caused by high temperatures, thus significantly improving the dissolution efficiency and bioavailability of active ingredients. White tea undergoes an endogenous enzyme-directed low-temperature anaerobic conversion process, significantly increasing the content of core active substances such as tea polysaccharides and total flavonoids, while simultaneously reducing the proportion of irritating tea polyphenols and caffeine. The entire process of low-temperature treatment avoids damage to active ingredients and significantly weakens the cold nature of white tea, addressing the pain points of ordinary white tea being too cold, easily irritating the spleen and stomach, and having low content of effective ingredients. Finally, a pulsed high-intensity light non-thermal low-temperature sterilization process is used, avoiding the active degradation caused by traditional high-temperature sterilization, and significantly improving the product's efficacy stability and shelf life. Detailed Implementation

[0018] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0019] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0020] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0021] Example 1 The Dendrobium officinale leaf tea composition of this embodiment, used to assist in lowering blood sugar and nourishing yin and moisturizing dryness, consists of the following raw materials by weight: 20 parts Dendrobium officinale and 50 parts white tea.

[0022] Among them, Dendrobium officinale is a fine powder that has undergone liquid nitrogen cryogenic embrittlement and low-temperature airflow cell wall breaking treatment, with a particle size D90≤5μm and a cell wall breaking rate ≥98% as tested. The preparation steps are as follows: take Dendrobium officinale stems, wash them, dry them at 41℃ with low-temperature hot air until the moisture content is 5.2%, and cut them into 4mm segments; place the Dendrobium segments in a liquid nitrogen cryogenic tank and cryogenically embrittle them at -18℃ for 20min; after embrittlement, immediately send them to a low-temperature airflow cell wall breaking machine and break them at -10℃, feed pressure 0.7MPa, and pulverization pressure 0.9MPa to obtain cell wall broken Dendrobium officinale fine powder, which is then passed through a 300-mesh sieve for later use.

[0023] White tea is a highly active white tea made from organic tea leaves with one bud and two leaves through a low-temperature anaerobic process involving the directional conversion of endogenous enzymes. The preparation steps are as follows: Take fresh white tea leaves and wither them naturally indoors for 60 hours until the moisture content of the fresh leaves reaches 40%; Place the withered tea leaves in a sealed anaerobic chamber and perform directional conversion of endogenous enzymes under anaerobic conditions of 22℃ and 65% relative humidity for 30 hours; After the conversion is completed, dry the tea leaves with low-temperature hot air at 38℃ until the moisture content reaches 5.0%, and then pulverize them to 40 mesh using a low-temperature airflow pulverizer to obtain white tea powder for later use.

[0024] The preparation method of the composition in this embodiment includes the following steps: S1. Raw material pretreatment: Weigh the pretreated Dendrobium powder and white tea powder according to the above weight proportions and set aside; S2. Homogeneous mixing: Dendrobium fine powder and white tea powder are placed in a three-dimensional motion mixer and mixed for 45 minutes in a Class 10,000 clean environment at 20℃ and 35% relative humidity until the material mixing uniformity RSD≤3% to obtain the composition mixing base material. S3. Low-temperature sterilization and molding: The mixed base material is sterilized using pulsed high-intensity light. The process parameters are: pulse width 300μs, pulse frequency 4Hz, single pulse energy 200J, xenon lamp double-sided flash mode, vertical distance between the xenon lamp and the material surface 10cm, material layer thickness 2.5mm, and 12 flashes per side. The entire sterilization process is temperature controlled by cold air circulation, and the material temperature is always ≤38℃. The relative humidity of the sterilization environment is 32%. After sterilization, the material moisture content is 6.2%, the total bacterial count is 820CFU / g, the total mold and yeast count is 45CFU / g, and coliform bacteria are not detected. It is then quantitatively packaged into tea bags of 5g each to obtain the Dendrobium large-leaf tea composition.

[0025] Example 2 The Dendrobium officinale leaf tea composition of this embodiment, used to assist in lowering blood sugar and nourishing yin and moisturizing dryness, consists of the following raw materials by weight: 10 parts Dendrobium officinale and 45 parts white tea.

[0026] The pretreatment process for Dendrobium officinale and white tea is the same as in Example 1, and the preparation method is the same as in Example 1.

[0027] Example 3 The Dendrobium officinale leaf tea composition of this embodiment, used to assist in lowering blood sugar and nourishing yin and moisturizing dryness, consists of the following raw materials by weight: 28 parts Dendrobium officinale and 35 parts white tea.

[0028] The pretreatment process for Dendrobium officinale and white tea is the same as in Example 1, and the preparation method is the same as in Example 1.

[0029] Example 4 The Dendrobium officinale leaf tea composition used in this embodiment for assisting in lowering blood sugar and nourishing yin and moisturizing dryness, by weight, consists of the following raw materials: 15 parts Dendrobium officinale, 40 parts white tea, 5 parts Polygonatum odoratum, 2 parts lily bulb, 2 parts Ophiopogon japonicus, 4 parts mulberry leaf, 2 parts kudzu root, and 1.5 parts bitter melon peptide powder.

[0030] The pretreatment process for Dendrobium officinale and white tea is the same as in Example 1; after removing impurities and washing Polygonatum odoratum, lily bulb, Ophiopogon japonicus, mulberry leaves and kudzu root, they are dried at a low temperature of 42℃ with hot air until the moisture content is 6.5%, pulverized to 50 mesh, and sieved to remove impurities before use; bitter gourd peptide powder is sieved through a 40-mesh sieve to remove impurities before use.

[0031] S1. Raw material pretreatment: Weigh the pretreated raw materials according to the above weight proportions and set aside; S2. Homogeneous mixing: Place all raw material powders into a three-dimensional motion mixer and mix for 50 minutes in a Class 10,000 clean environment at 20℃ and 35% relative humidity until the material mixing uniformity RSD ≤ 3%, to obtain the composition mixing base material; S3. Low-temperature sterilization and molding: The sterilization process is the same as in Example 1. After sterilization, the tea is packaged into tea bags in a quantitative manner of 5g per bag, thus obtaining the Dendrobium large-leaf tea composition.

[0032] Comparative Example 1 The only difference between this comparative example and Example 1 is that the Dendrobium officinale was pulverized using a common universal pulverizer and passed through a 300-mesh sieve, and the liquid nitrogen cryogenic embrittlement-low temperature airflow cell wall breaking treatment was not used. All other raw materials, proportions and preparation methods are the same as in Example 1.

[0033] Comparative Example 2 The only difference between this comparative example and Example 1 is that the white tea is prepared using the ordinary white tea preparation process, that is, the fresh leaves are naturally withered for 72 hours and then dried at 100℃. The endogenous enzyme-directed low-temperature anaerobic conversion treatment is not used. The other raw materials, proportions and preparation methods are the same as those in Example 1.

[0034] Comparative Example 3 The only difference between this comparative example and Example 1 is that the sterilization process uses traditional high-temperature moist heat sterilization at 121°C for 15 minutes. The other raw materials, proportions, and preparation methods are the same as in Example 1.

[0035] Comparative Example 4 This comparison is based on a commercially available brand of Dendrobium officinale white tea bags, with Dendrobium officinale and Fuding white tea as the core ingredients, and no other auxiliary ingredients.

[0036] Comparative Example 5 All raw materials in the formulation of Example 4 (except bitter melon peptide powder) were combined, decocted twice at 100°C for 1 hour each time, and 10 times the amount of water was added. The decoctions were combined and concentrated into an extract. After mixing with bitter melon peptide powder, the extract was spray-dried to form powder with an air inlet temperature of 180°C, and then packaged.

[0037] Experimental Example 1: Test for Dissolution and Retention Rate of Active Ingredients 1. Test sample Composition samples obtained in Examples 1-4; composition samples obtained in Comparative Examples 1-5.

[0038] 2. Testing Items and Methods (1) Detection of active ingredient retention rate The contents of Dendrobium polysaccharides, Dendrobium alkaloids, tea polysaccharides, and total flavonoids in the sterilized finished product were measured separately and compared with the initial contents of the corresponding components in the mixed base material before sterilization. The retention rate was calculated according to the formula: Retention rate (%) = (Component content after sterilization / Initial component content before sterilization) × 100% Dendrobium polysaccharides: Detected according to the phenol-sulfuric acid method under the Dendrobium officinale section of the Chinese Pharmacopoeia; Dendrobine: Detected according to the high performance liquid chromatography (HPLC) method specified in the Chinese Pharmacopoeia; Tea polysaccharides: Detected using the phenol-sulfuric acid method (excluding monosaccharide interference); Total flavonoids: determined by the aluminum nitrate-sodium nitrite colorimetric method; Simultaneously, the total tea polyphenols, catechin components, and free caffeine content in the finished products of Example 1 and Comparative Example 2 were detected: Total tea polyphenol content: determined according to GB / T 8313-2018 "Determination Method of Tea Polyphenol and Catechin Content in Tea"; Catechin components (ester-type catechins EGCG, ECG, GCG, and non-ester-type catechins EGC, EC): detected by high performance liquid chromatography (HPLC); Free caffeine content: Detected by HPLC method according to GB / T 8312-2013 "Determination of Caffeine in Tea".

[0039] (2) Detection of dissolution rate of active ingredients Take 5g of each sample, add 200mL of boiling water at 100℃, seal and let stand for 5 minutes, filter and take the clear tea soup, detect the content of Dendrobium polysaccharides and tea polysaccharides in the tea soup, compare with the total content of the corresponding components in the sample, and calculate the dissolution rate according to the formula: Dissolution rate (%) = (Amount of components dissolved in tea infusion / Total content of components in the sample) × 100% 3. Test Results Table 1. Results of retention rate of active ingredients in each test sample (%, n=3)

[0040] Table 2 Comparison of the content and proportion of the core irritating components in the two groups of white tea

[0041] Table 3. Results of dissolution rate of active ingredients in each test sample (%, n=3)

[0042] 4. Results Analysis The retention rates of Dendrobium polysaccharides, Dendrobium alkaloids, tea polysaccharides, and total flavonoids in Examples 1-4 of this invention were consistently above 94%, far superior to all comparative examples. Among them, the core active ingredients in Comparative Examples 2, 3, and 5 showed significant degradation, proving that the low-temperature preparation process combined with pulsed light non-thermal sterilization system of this invention can achieve ultimate protection of the highly heat-sensitive active ingredients in Dendrobium and white tea, completely solving the problem of significant degradation of active ingredients caused by traditional high-temperature processes.

[0043] This invention's process does not cause any loss of total tea polyphenols. It only uses the targeted hydrolysis of endogenous enzymes to convert highly stimulating and bitter ester-type catechins into mild and non-stimulating non-ester-type catechins. Without losing the core active substances of tea, it significantly reduces the stimulating effect of tea polyphenols, thus weakening the cold nature of white tea from the root. At the same time, the activated endogenous glycosidase converts some of the free caffeine with central nervous system excitation into non-stimulating bound caffeine glycosides, reducing the proportion of free caffeine and avoiding problems such as palpitations, insomnia, and sympathetic nervous system excitation that are easily caused by ordinary white tea. This makes it suitable for the long-term drinking needs of people with high blood sugar.

[0044] The dissolution rates of Dendrobium polysaccharides in Examples 1-4 of this invention are ≥88.6% and ≥92.7%, respectively, while those in Comparative Example 1 are only 42.6% and Comparative Example 4 is 38.5%. This fully demonstrates that the liquid nitrogen cryogenic embrittlement-low-temperature airflow cell wall breaking treatment used in this invention can completely break down the tough cell walls of Dendrobium, allowing the polysaccharide components to be almost completely released during brewing, significantly improving bioavailability. Comparative Example 2 showed a tea polysaccharide retention rate of only 82.3%, a total flavonoid retention rate of only 78.5%, and a tea polysaccharide dissolution rate of only 65.4%. This indicates that the endogenous enzyme-directed low-temperature anaerobic transformation treatment of the present invention directionally hydrolyzes the bound flavonoids and polysaccharide precursors in tea leaves into free active tea polysaccharides and total flavonoids, achieving targeted enrichment of core functional components. This not only effectively protects the active substances in white tea but also reduces losses during subsequent drying through enzymatic transformation, thereby achieving higher component retention. It also gently hydrolyzes the cellulose and pectin structures of tea cell walls, breaking down cell barriers and making the functional components within the cells more easily dissolved during brewing, significantly improving bioavailability.

[0045] Comparative Example 5 used a traditional water decoction + high-temperature spray drying process. The retention rate and dissolution rate of all core active ingredients were the lowest among all samples, proving that the low-temperature preparation system of this invention has a better protection effect on the efficacy components compared with the traditional Chinese medicine extraction and granulation process.

[0046] Experimental Example 2: In vitro α-glucosidase inhibitory activity assay 1. Test sample Composition samples obtained in Examples 1 and 4; composition samples obtained in Comparative Examples 1-5.

[0047] Sample pretreatment: Each sample was brewed with boiling water at 100℃ at a material-to-liquid ratio of 1:50 (g / mL) for 5 minutes. The clear tea liquor was filtered and freeze-dried to obtain sample powder. The sample powder was prepared with 0.2mol / L pH 6.8 phosphate buffer to form a test solution with a concentration of 10mg / mL and stored at 4℃ for later use.

[0048] 2. Test Methods Using the DNS method (3,5-dinitrosalicylic acid method), samples were added to a 96-well microplate according to the following system: (1) Blank control group: Add 40 μL phosphate buffer + 20 μL 0.2 U / mL α-glucosidase solution, incubate at 37℃ for 10 min, add 20 μL 10 mmol / L PNPG (4-nitrophenyl-α-D-glucopyranoside) substrate, incubate at 37℃ for 30 min, and add 100 μL 0.4 mol / L sodium carbonate solution to terminate the reaction; (2) Sample group: Add 40 μL of test solution + 20 μL of α-glucosidase solution, and perform the remaining operations as in the blank control group; (3) Background group: Add an equal volume of buffer solution to the corresponding group to replace the enzyme solution and eliminate the interference of the sample background absorbance.

[0049] The absorbance at 405 nm was measured using an ELISA reader, and the α-glucosidase inhibition rate was calculated using the following formula: Inhibition rate (%) = [1 - (Absorbance of sample group - Absorbance of sample background group) / (Absorbance of blank control group - Absorbance of blank background group)] × 100% 3. Test Results Table 4. Inhibition rate of α-glucosidase by each test sample (n=3, x±s)

[0050] 4. Results Analysis The inhibition rates of α-glucosidase in Examples 1 and 4 of this invention reached 72.5% and 83.6%, respectively, which were significantly higher than those in all comparative examples (P<0.05). This demonstrates that the composition of this invention has extremely strong α-glucosidase inhibitory activity, can effectively delay the absorption of carbohydrates in the intestine, and has excellent potential for postprandial blood glucose control.

[0051] The inhibition rate of Example 4 was significantly higher than that of Example 1, proving that excipients such as mulberry leaves, kudzu root, and bitter melon peptide powder can form a significant synergistic effect in lowering blood sugar with Dendrobium officinale and white tea, further enhancing the auxiliary effect in lowering blood sugar.

[0052] The inhibition rates of Comparative Examples 1-5 were significantly lower than those of the Example. The main reasons are as follows: In Comparative Example 1, the dendrobium cell wall was not sufficiently broken, resulting in insufficient dissolution of active ingredients; in Comparative Example 2, the dissolution rate of tea polysaccharides was reduced, which confirmed the important role of the endogenous enzyme-directed low-temperature anaerobic conversion process in enhancing the hypoglycemic activity of white tea; in Comparative Examples 3 and 5, the high-temperature process led to a large amount of degradation of hypoglycemic active ingredients such as tea polysaccharides and flavonoids, which in turn confirmed the advantages of the pulsed strong light low-temperature sterilization process of this invention in protecting heat-sensitive active ingredients; and in Comparative Example 4, the commercially available product had no process optimization, and the content and dissolution rate of active ingredients were extremely low.

[0053] Experimental Example 3: Animal Efficacy Study of Assisted Hypoglycemic Agent 1. Test materials Experimental animals: SPF-grade male ICR mice, weighing 20±2g, acclimatized for 3 days, with free access to food and water, ambient temperature of 22±2℃, and 12h light-dark cycle.

[0054] Reagents and instruments: streptozotocin (STZ, Sigma); blood glucose test strips, blood glucose meter (Roche); mouse insulin (INS) ELISA kit (Nanjing Jiancheng Biotechnology); high-speed refrigerated centrifuge, enzyme-linked immunosorbent assay (ELISA) reader.

[0055] 2. Test Methods (1) Establishment of a mouse model of type 2 diabetes mellitus (T2DM) Mice were first fed a high-sugar, high-fat diet for 4 weeks, then fasted for 12 hours but allowed free water, and then injected intraperitoneally with 35 mg / kg STZ (prepared with citrate buffer, pH 4.5). After continuing the high-sugar, high-fat diet for 1 week, fasting was continued for 8 hours but free water was allowed, and fasting blood glucose was measured. A fasting blood glucose level ≥11.1 mmol / L was considered a successful model establishment criterion.

[0056] (2) Trial grouping and administration Mice that successfully developed the model were randomly divided into 9 groups of 10 mice each, as follows: Normal control group: fed with normal feed and administered an equal volume of physiological saline by gavage daily; Model group: fed with high-sugar and high-fat diet, and administered an equal volume of physiological saline by gavage daily; Example 1 group: fed with high sugar and high fat diet, and administered the sample solution of Example 1 by gavage daily at a dose of 2 g / kg·d (calculated based on crude drug amount); Example 4 group: fed with high sugar and high fat diet, and administered the sample solution of Example 4 by gavage daily at a dose of 2 g / kg·d (calculated based on the amount of raw drug). Comparative groups 1-5: fed with high-sugar and high-fat diets, and administered corresponding sample solutions by gavage daily at a dose of 2 g / kg·d (based on crude drug amount).

[0057] All groups received continuous oral administration for 4 weeks, during which time they had free access to food and water, and their weight and food intake were recorded weekly.

[0058] (3) Detection indicators Fasting blood glucose (FBG): Before administration, 2 weeks after administration, and 4 weeks after administration, mice were fasted for 8 hours but allowed free access to water, and fasting blood glucose levels were measured by blood collection from the tail vein. Serum insulin and insulin resistance index: Four weeks after drug administration, blood was collected from the eyeballs of mice, serum was separated, and serum insulin (INS) levels were measured using an ELISA kit. The insulin resistance index (HOMA-IR) was calculated according to the homeostasis model formula. HOMA-IR = (fasting blood glucose mmol / L × fasting insulin mIU / L) / 22.5 3. Test Results Table 5 Fasting blood glucose levels of mice in each group at different time points (mmol / L, n=10, x±s)

[0059] Note: Compared to the model group, * P<0.05; compared with all comparative groups, # P<0.05.

[0060] Table 6 Insulin levels and insulin resistance index in mice after 4 weeks of drug administration (n=10, x±s)

[0061] Note: Compared to the model group, * P<0.05; compared with all comparative groups, * P<0.05.

[0062] 4. Results Analysis Four weeks after administration, the fasting blood glucose levels of mice in groups 1 and 4 of this invention were significantly lower than before administration, and significantly lower than those in the model group and all comparative groups (P<0.05), demonstrating that the composition of this invention has excellent in vivo adjuvant hypoglycemic effect, which is time-dependent.

[0063] In Examples 1 and 4, the serum insulin levels of mice were significantly increased and the insulin resistance index was significantly decreased, showing significant differences compared with the model group and the control group (P<0.05). This demonstrates that the composition of the present invention can effectively protect the pancreatic β-cell function of diabetic mice, promote insulin secretion, and significantly improve the core insulin resistance problem of type 2 diabetes, thereby regulating glucose metabolism disorder from the root.

[0064] The blood glucose and insulin levels of mice in the five comparative groups were not significantly different from those in the model group (P>0.05), and there was no obvious hypoglycemic effect. This proves that the traditional high-temperature water decoction + spray drying process completely destroyed the core hypoglycemic active ingredients of the composition and lost its efficacy. The other comparative groups showed only a very weak hypoglycemic effect, which was far less than that of the group in the embodiment of the present invention.

[0065] Experimental Example 4: Efficacy Test of Yin-Nourishing and Dryness-Moistening Drugs 1. Test materials Experimental animals: SPF-grade female ICR mice, weighing 20±2g, acclimatized for 3 days, with free access to food and water, ambient temperature of 22±2℃, and 12h light-dark cycle.

[0066] Reagents and instruments: thyroxine tablets, reserpine (Sinopharm Group); mouse cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) ELISA kits (Nanjing Saihongrui Biotechnology); electronic balance, high-speed refrigerated centrifuge, enzyme-linked immunosorbent assay (ELISA) reader.

[0067] 2. Test Methods (1) Establishment of a mouse model of yin deficiency and dryness-heat Mice were administered 150 mg / kg / day of thyroxine suspension and 0.5 mg / kg / day of reserpine suspension daily by gavage for 14 consecutive days to establish a yin deficiency and heat model. Successful model establishment was defined as mice exhibiting typical symptoms of yin deficiency and heat, including significantly increased water intake, weight loss, irritability, dry fur, and increased heart rate, showing significant differences from the normal group.

[0068] (2) Trial grouping and administration Mice that successfully developed the model were randomly divided into 9 groups of 10 mice each, as follows: Normal control group: administered an equal volume of normal saline by gavage daily; Model group: administered an equal volume of physiological saline by gavage daily; Example 1 group: The sample solution of Example 1 was administered by gavage daily at a dose of 2 g / kg·d (based on the amount of raw drug). Example 4 group: The sample solution of Example 4 was administered by gavage daily at a dose of 2 g / kg·d (based on the amount of raw drug). Comparative Examples 1-5: The corresponding control sample solution was administered by gavage daily at a dose of 2 g / kg. d (based on the amount of raw medicinal material).

[0069] All groups were administered the drug by gavage for 21 consecutive days. During this period, the average daily water intake and weight changes of mice in each group were recorded daily, and they had free access to food and water.

[0070] (3) Detection indicators Basic indicators: During the administration period, the average daily water intake and body weight of mice in each group were recorded, and the change in body weight after 21 days of administration was statistically analyzed. Core indicators of Yin deficiency syndrome: 21 days after administration, blood was collected from the eyeballs of mice, serum was separated, and serum cAMP and cGMP levels were measured using an ELISA kit. The cAMP / cGMP ratio was calculated (the core biochemical indicator of Yin deficiency syndrome in Traditional Chinese Medicine, which is characterized by elevated cAMP, decreased cGMP, and a significantly elevated cAMP / cGMP ratio).

[0071] 3. Test Results Table 7. Changes in water intake and body weight of mice in each group after 21 days of drug administration (n=10, x±s)

[0072] Note: Compared to the model group, * P<0.05; compared with all comparative groups, # P<0.05.

[0073] Table 8. Serum cAMP and cGMP levels and ratios in mice after 21 days of drug administration (n=10, x±s)

[0074] Note: Compared to the model group, * P<0.05; compared with all comparative groups, # P<0.05 4. Results Analysis After 21 days of administration, the core symptoms of yin deficiency and dryness-heat in mice in Examples 1 and 4 of this invention were significantly reversed: the average daily water intake decreased by 49.5% and 57.3% respectively compared with the model group, and the weight gain changed from negative to positive growth in the model group. The differences were significant compared with the model group and all comparative groups (P<0.05), proving that the composition of this invention can significantly improve the core symptoms of yin deficiency and dryness-heat such as thirst, excessive drinking, and emaciation, and has excellent effects of promoting body fluid production and moisturizing dryness.

[0075] In Examples 1 and 4, the serum cAMP levels of mice were significantly decreased, while the cGMP levels were significantly increased. The cAMP / cGMP ratio decreased from 6.27 in the model group to 2.42 and 1.85, respectively, approaching the level of the normal control group, and was significantly different from all comparative groups (P<0.05). The cAMP / cGMP ratio is the gold standard biochemical indicator for Yin deficiency syndrome in Traditional Chinese Medicine. This result directly proves that the composition of the present invention can correct the endocrine disorders of Yin deficiency constitution at the molecular level, fundamentally improve Yin deficiency and dryness syndrome, and achieve the effect of nourishing Yin and moisturizing dryness by addressing both the symptoms and the root cause.

[0076] The five comparative groups of mice showed no significant differences in any of their indicators compared to the model group (P>0.05), and had no yin-nourishing and dryness-moistening effects. The other comparative groups showed only very weak improvement effects, far less than those in the embodiments of this invention. This proves that the scientific formulation and exclusive low-temperature preparation process of this invention are the key to achieving the core efficacy of nourishing yin and moistening dryness.

[0077] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A Dendrobium officinale large-leaf tea composition for assisting in lowering blood sugar and nourishing yin and moisturizing dryness, characterized in that, By weight, the main ingredients include: 8-32 parts Dendrobium and 25-65 parts white tea.

2. The Dendrobium officinale large-leaf tea composition as described in claim 1, characterized in that, By weight, the main ingredients include: 10-28 parts Dendrobium and 35-45 parts white tea.

3. The Dendrobium officinale large-leaf tea composition as described in claim 1 or 2, characterized in that, The dendrobium mentioned is Dendrobium officinale, specifically fine powder that has undergone liquid nitrogen cryogenic embrittlement and low-temperature airflow cell disruption treatment, with a particle size D90≤5μm.

4. The Dendrobium officinale large-leaf tea composition as described in claim 3, characterized in that, The specific steps for obtaining the fine powder after liquid nitrogen cryogenic embrittlement and low-temperature airflow cell wall breaking treatment are as follows: Take the stems of Dendrobium officinale, wash them, dry them with low-temperature hot air at 40-42℃ until the moisture content is ≤6%, and cut them into 3-5mm segments; place the Dendrobium segments in a liquid nitrogen cryogenic tank and cryogenically embrittle them at -20~-15℃ for 15-25 minutes; after embrittlement, immediately send them into a low-temperature airflow cell wall breaking machine and break them at -12~-8℃, feed pressure 0.6-0.8MPa, and pulverizing pressure 0.8-1.0MPa to obtain cell wall broken Dendrobium fine powder, which is then passed through a 200-400 mesh sieve for later use.

5. The Dendrobium officinale large-leaf tea composition as described in claim 1 or 2, characterized in that, The white tea is a highly active white tea made from organic tea leaves with one bud and two leaves through a targeted low-temperature anaerobic transformation process using endogenous enzymes.

6. The Dendrobium officinale large-leaf tea composition as described in claim 5, characterized in that, The specific steps of the endogenous enzyme-directed low-temperature anaerobic transformation treatment are as follows: Take fresh white tea leaves and wither them naturally indoors for 54-66 hours until the moisture content of the fresh leaves is 38-42%; place the withered tea leaves in a sealed anaerobic chamber and, under the conditions of temperature 21-24℃ and relative humidity 62-68%, anaerobically activate endogenous polyphenol oxidase and glycosidase for directed transformation for 24-36 hours; after the transformation is completed, dry the tea leaves with low-temperature hot air at 36-39℃ until the moisture content is ≤5.5%, and then pulverize them to 30-60 mesh using a low-temperature airflow pulverizer to obtain white tea powder for later use.

7. The Dendrobium officinale large-leaf tea composition as described in claim 1, characterized in that, The composition also includes the following raw materials in parts by weight: 2-8 parts of Solomon's seal rhizome, 1-4 parts of lily bulb, 1-3 parts of Ophiopogon japonicus, 2-6 parts of mulberry leaf, 1-4 parts of kudzu root, and 1-2 parts of bitter melon peptide powder.

8. The method for preparing the Dendrobium officinale large-leaf tea composition according to any one of claims 1-7, characterized in that, Includes the following steps: S1. Pretreatment: Take Solomon's seal, lily bulb, ophiopogon japonicus, mulberry leaf, and kudzu root, remove impurities, wash them, dry them at a low temperature of 40~45℃ with hot air until the moisture content is ≤7%, pulverize them to 40~60 mesh, sieve to remove impurities, and set them aside for use. S2. Homogeneous mixing: Dendrobium fine powder, white tea powder, bitter melon peptide powder and the powder obtained in S1 are placed into a three-dimensional motion mixer and mixed for 30-60 minutes at 15-25℃ and relative humidity ≤40% until the mixture is uniform to obtain the composite mixture base material. S3. Low-temperature sterilization and molding: The mixed base material is sterilized at a low temperature without heat. The moisture content of the material after sterilization is controlled to be ≤7.0%, the total number of colonies is ≤1000 CFU / g, and the total number of molds and yeasts is ≤100 CFU / g. Then, it is quantitatively packaged according to the target tea beverage dosage form to obtain the Dendrobium large-leaf tea composition.

9. The method for preparing the Dendrobium officinale large-leaf tea composition as described in claim 8, characterized in that, The non-thermal low-temperature sterilization described in step S3 is pulsed high-intensity light sterilization, with a pulse width of 250-350μs, a pulse frequency of 3-5Hz, and a single pulse energy of 150-250J. It uses a xenon lamp with a double-sided flashing mode, a vertical distance of 8-12cm from the material surface, a material layer thickness of 2-3mm, and 8-15 flashes per side, each lasting 2-4 minutes. The entire sterilization process is temperature-controlled by cold air circulation, ensuring that the material temperature is always ≤40℃ and the relative humidity of the sterilization environment is ≤35%.

10. The use of the Dendrobium officinale white tea composition according to any one of claims 1-7 or the Dendrobium officinale large-leaf tea composition prepared by the preparation method of claim 8 or 9 in the preparation of products that help lower blood sugar and improve yin deficiency and dryness syndrome.

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