Traditional Chinese medicine composition for treating senile dementia and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating senile dementia and a preparation method thereof. The invention discloses a traditional Chinese medicine composition for treating senile dementia. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 23-28 parts of rhizoma coptidis, 20-25 parts of cortex cinnamomi, 18-22 parts of herba lysimachiae, 13-19 parts of rhizoma acori graminei, 15-20 parts of radix polygalae, 9-13 parts of radix rehmanniae praeparata, 6-12 parts of caulis sargentodoxae, 7-11 parts of poria cocos and 3-8 parts of radix glycyrrhizae. Test results show that by taking the traditional Chinese medicine composition for treating the senile dementia, the learning and memory ability of a senile dementia model mouse can be remarkably improved, A beta deposition in the brain can be reduced, the safety is good, and a novel traditional Chinese medicine scheme which is safe, effective and clear in mechanism is provided for the senile dementia.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a traditional Chinese medicine composition for treating Alzheimer's disease and its preparation method. Background Technology

[0002] Senile dementia is a group of clinical syndromes characterized by cognitive decline, with Alzheimer's disease (AD) being the most common cause. The disease has an insidious onset and progresses progressively, mainly manifesting as memory loss, language impairment, executive function decline, behavioral abnormalities, and personality changes. In its late stages, it can lead to complete loss of self-care ability, placing significant care and economic burdens on families and society.

[0003] Currently, Western medicine treatments mainly include cholinesterase inhibitors and NMDAR receptor antagonists. However, these chemical drugs generally have limited efficacy and certain degrees of toxic side effects, restricting their widespread clinical application. Compared to Western medicine's symptom-oriented treatment model, Traditional Chinese Medicine (TCM) emphasizes a holistic approach and the principle of "treating the root cause," giving it a significant advantage in treating Alzheimer's disease. Modern research shows that TCM compositions can improve patients' cognitive function and clinical symptoms through multi-target and multi-pathway mechanisms of action, while simultaneously strengthening the body's resistance and eliminating pathogenic factors, exhibiting holistic regulation and relatively fewer side effects.

[0004] Based on the above objectives, the present invention provides a traditional Chinese medicine composition for treating Alzheimer's disease and its preparation method, so as to overcome the technical defects of existing chemical drugs, give full play to the overall regulatory advantages of traditional Chinese medicine, and have important clinical value and social significance for enriching the clinical treatment methods of Alzheimer's disease and improving the prognosis of patients. Summary of the Invention

[0005] The first objective of this invention is to provide a traditional Chinese medicine composition for treating Alzheimer's disease.

[0006] The second objective of this invention is to provide a method for preparing a traditional Chinese medicine composition for treating Alzheimer's disease.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A traditional Chinese medicine composition for treating Alzheimer's disease, the composition comprising the following raw materials in parts by weight: 23-28 parts Coptis chinensis, 20-25 parts Cinnamomum cassia, 18-22 parts Rhizoma Citri Reticulatae, 13-19 parts Acorus tatarinowii, 15-20 parts Polygala tenuifolia, 9-13 parts Rehmanniae Radix Rehmanniae, 6-12 parts Clematis chinensis, 7-11 parts Poria cocos, and 3-8 parts Glycyrrhiza uralensis.

[0008] Furthermore, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 25 parts Coptis chinensis, 22 parts Cinnamomum cassia, 20 parts Rhizoma Citri Reticulatae, 16 parts Acorus tatarinowii, 17 parts Polygala tenuifolia, 11 parts Rehmanniae Radix Praeparata, 9 parts Stellariae Aristolochiae, 9 parts Poria cocos, and 5 parts Glycyrrhiza uralensis.

[0009] The preparation method of the traditional Chinese medicine composition for treating Alzheimer's disease as described above specifically includes the following steps: (1) Weigh out each ingredient according to the weight proportions: Coptis chinensis, cinnamon, golden thread, Acorus tatarinowii, Polygala tenuifolia, Rehmannia glutinosa, Fragrant Vine, Poria cocos, and Glycyrrhiza uralensis. Grind them into powder and sieve them to obtain Chinese medicine powder for later use. (2) Soak the Chinese medicine powder prepared in step (1) in water, then decoct it; filter, centrifuge, and collect the first filtrate and the first filter residue; (3) Then add water to the first filter residue to soak, then boil; filter, centrifuge and collect the second filtrate, combine the first filtrate and the second filtrate; concentrate; dry to obtain the final product.

[0010] Furthermore, the mesh size of the sieve in step (1) is 60-80.

[0011] Furthermore, in step (2), the amount of water added is 8-12 times the total weight of the Chinese medicine powder; the soaking time is 1-3 hours; and the decoction time is 2-3 hours.

[0012] Furthermore, in step (3), the amount of water added is 6-10 times the total weight of the first filter residue; the soaking time is 1-3 hours; and the decocting time is 1-2 hours.

[0013] Furthermore, in step (3), the concentration temperature is 50-70℃, and the concentration is carried out to a thick paste with a relative density of 1.20-1.26.

[0014] Furthermore, the drying temperature in step (3) is 50-60°C.

[0015] To facilitate a better understanding of this invention, the effects of each ingredient are explained below: Coptis chinensis: Bitter, cold. It enters the heart, spleen, stomach, liver, gallbladder, and large intestine meridians. Functions and indications: Clears heat and dries dampness, drains fire and detoxifies. According to *Mingyi Bielu*: Slightly cold, non-toxic. It treats cold and heat in the five internal organs, chronic diarrhea with pus and blood, stops thirst, relieves fright, eliminates water retention, benefits bones, regulates the stomach, thickens the intestines, benefits the gallbladder, and treats mouth ulcers.

[0016] Cinnamon: pungent, sweet, and very hot in nature. It enters the kidney, spleen, heart, and liver meridians. Functions and indications: warms the middle jiao and tonifies the kidneys, warms and unblocks the meridians. The *Shennong Bencao Jing* records: "It is pungent and warm in nature, treats a hundred diseases, nourishes the spirit, improves complexion, benefits joints, and tonifies the middle jiao and replenishes qi. It acts as a guide for other medicines, and long-term use leads to spiritual enlightenment, lightness of body, and longevity. It gives the face a radiant glow, and the eyebrows remain youthful."

[0017] Golden thread: Sweet, bitter, slightly cold. It enters the liver, kidney, and heart meridians. Functions and indications: Tonifies the kidneys and strengthens yang, unblocks meridians and promotes blood circulation. Recorded in the *National Compendium of Chinese Herbal Medicine*: Tonifies the kidneys and strengthens yang.

[0018] Sweet flag (Acorus calamus): pungent, bitter, and warm. It enters the Heart and Stomach meridians. Functions and indications: It improves intelligence and refreshes the mind, resolves dampness and stimulates appetite. The *Mingyi Bielu* records: Long-term use improves hearing and vision, and benefits the mind.

[0019] Polygala tenuifolia: Bitter, pungent, warm. Enters the heart, kidney, and lung meridians. Functions and indications: Calms the mind and relieves depression, opens the orifices and resolves phlegm. The *Treatise on the Nature of Medicinal Herbs* records: Treats forgetfulness, calms the soul, prevents confusion, strengthens yang, and treats nightmares.

[0020] Rehmannia glutinosa (processed): Sweet, slightly warm. Enters the liver and kidney meridians. Functions and indications: Nourishes blood and yin, replenishes essence and marrow. The Compendium of Materia Medica records: It fills bone marrow, promotes muscle growth, generates essence and blood, replenishes deficiencies in the five internal organs, unblocks blood vessels, benefits the ears and eyes, darkens hair, treats five types of fatigue and seven types of injury in men, and treats uterine bleeding, irregular menstruation, and various pregnancy and childbirth-related illnesses in women.

[0021] Fragrant Vine: Pungent, slightly bitter, slightly warm. Enters the Liver and Spleen meridians. Functions and indications: Promotes blood circulation and regulates Qi, invigorates blood and relieves pain. Recorded in *Guizhou Folk Medicine*: Promotes blood circulation and regulates Qi.

[0022] Poria cocos: Sweet, bland, and neutral. It enters the heart, lung, spleen, and kidney meridians. Functions and indications: Strengthens the spleen and calms the mind, eliminates dampness and resolves phlegm. The *Shennong Bencao Jing* records: It treats chest and rib pain, anxiety, fright, palpitations, epigastric pain, chills and fever, cough, dry mouth and tongue, and promotes urination.

[0023] Licorice: Sweet in taste and neutral in nature. It enters the Heart, Lung, Spleen, and Stomach meridians. Functions and indications: Tonifies the spleen and replenishes qi, clears heat and detoxifies, resolves phlegm and relieves cough, alleviates spasms and pain, and harmonizes other herbs. The Compendium of Materia Medica records that licorice is the chief herb among all medicines, treating seventy-two kinds of mineral poisoning, detoxifying one thousand two hundred kinds of herbal poisoning, and effectively harmonizing other herbs; hence, it is known as the "Elder of the Nation."

[0024] Compared with the prior art, the main advantages of the present invention are: This invention provides a traditional Chinese medicine composition for treating Alzheimer's disease and its preparation method. The composition uses Coptis chinensis, Cinnamomum cassia, and Lysimachia christinae as the principal herbs, addressing both the root cause and symptoms. Its core principle lies in harmonizing the heart and kidneys, clearing heat from the upper body and warming the lower body, and tonifying the kidneys and strengthening yang, directly targeting the core pathogenesis of "heart-kidney disharmony." The auxiliary herbs are Acorus tatarinowii for improving intelligence and refreshing the mind, Polygala tenuifolia for opening the orifices, resolving phlegm, and calming the mind, and Rehmannia glutinosa for nourishing blood, yin, and replenishing essence and marrow, enhancing the kidney-tonifying, orifice-opening, and intelligence-enhancing effects of the principal herbs. The adjuvant herbs are Cynanchum paniculatum for promoting blood circulation and regulating qi, and Poria cocos for strengthening the spleen, calming the mind, and eliminating dampness and phlegm, promoting blood circulation and unobstructed brain vessels. The guiding herb is Glycyrrhiza uralensis to harmonize the other herbs, mitigating the hot and cold properties associated with sexual activity, and ensuring the overall balance of the formula's medicinal properties. The traditional Chinese medicine composition provided by this invention is meticulously formulated, with clear distinctions between the principal, assistant, adjuvant, and guiding herbs, working together to harmonize the heart and kidneys, resolve phlegm, open the orifices, tonify the kidneys and replenish essence, and invigorate blood and regulate qi, treating the underlying deficiency and superficial excess symptoms of Alzheimer's disease through multiple targets and pathways. The experimental results show that the traditional Chinese medicine composition prepared by this invention can significantly improve the learning and memory abilities of Alzheimer's disease model mice, reduce Aβ deposition in the brain, and has good safety, providing a safe, effective and well-defined new traditional Chinese medicine solution for Alzheimer's disease. Attached Figure Description

[0025] Figure 1 The effects of different traditional Chinese medicine compositions used to treat Alzheimer's disease on the protein expression level of PSEN1; Figure 2 The effects of different traditional Chinese medicine compositions used to treat Alzheimer's disease on the protein expression level of BACE1; Figure 3 To investigate the effects of different traditional Chinese medicine compositions used to treat Alzheimer's disease on the protein expression level of C83 / APP. Detailed Implementation

[0026] The technical solution of the present invention will be further described below with reference to specific embodiments. However, those skilled in the art should understand that the following embodiments are only for illustrating the present invention and should not be regarded as limiting the present invention. Specific conditions not specified in the embodiments are performed according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the reagents or instruments used are all conventional products obtained through commercial channels.

[0027] Example 1 A traditional Chinese medicine composition for treating Alzheimer's disease, the composition comprising the following raw materials in parts by weight: 25 parts Coptis chinensis, 22 parts Cinnamomum cassia, 20 parts Rhizoma Citri Reticulatae, 16 parts Acorus tatarinowii, 17 parts Polygala tenuifolia, 11 parts Rehmanniae Radix Praeparata, 9 parts Stellariae Aristolochiae, 9 parts Poria cocos, and 5 parts Glycyrrhiza uralensis.

[0028] A method for preparing a traditional Chinese medicine composition for treating Alzheimer's disease specifically includes the following steps: (1) Weigh out each ingredient according to the following weight proportions: 25 parts Coptis chinensis, 22 parts Cinnamomum cassia, 20 parts Eupatorium fortunei, 16 parts Acorus tatarinowii, 17 parts Polygala tenuifolia, 11 parts Rehmannia glutinosa, 9 parts Clematis armandii, 9 parts Poria cocos, and 5 parts Glycyrrhiza uralensis. After crushing, pass the powder through a 70-mesh sieve to obtain Chinese medicine powder for later use. (2) Soak the Chinese medicine powder prepared in step (1) in water equal to 10 times the total weight of the Chinese medicine powder for 2 hours, then decoct for 2 hours; filter, centrifuge, and collect the first filtrate and the first filter residue; (3) Then add water equal to 8 times the total weight of the first filter residue to the first filter residue and soak for 2 hours, then boil for 1 hour; after filtration and centrifugation, collect the second filtrate, and combine the first filtrate and the second filtrate; concentrate under reduced pressure at a temperature of 60℃ and a pressure of -0.09MPa to a thick paste with a relative density of 1.23; dry under reduced pressure at a temperature of 55℃ and a pressure of -0.1MPa to obtain the final product.

[0029] Example 2 A traditional Chinese medicine composition for treating Alzheimer's disease, the composition comprising the following raw materials in parts by weight: 23 parts Coptis chinensis, 20 parts Cinnamomum cassia, 18 parts Rhizoma Citri Reticulatae, 13 parts Acorus tatarinowii, 15 parts Polygala tenuifolia, 9 parts Rehmanniae Radix Praeparata, 6 parts Clematis chinensis, 7 parts Poria cocos, and 3 parts Glycyrrhiza uralensis.

[0030] A method for preparing a traditional Chinese medicine composition for treating Alzheimer's disease specifically includes the following steps: (1) Weigh out each ingredient according to the following weight proportions: 23 parts Coptis chinensis, 20 parts Cinnamomum cassia, 18 parts Eupatorium fortunei, 13 parts Acorus tatarinowii, 15 parts Polygala tenuifolia, 9 parts Rehmannia glutinosa, 6 parts Clematis armandii, 7 parts Poria cocos, and 3 parts Glycyrrhiza uralensis. After crushing, pass the powder through a 60-mesh sieve to obtain Chinese medicine powder for later use. (2) Soak the Chinese medicine powder prepared in step (1) in water equal to 8 times the total weight of the Chinese medicine powder for 1 hour, then decoct for 2 hours; filter, centrifuge, and collect the first filtrate and the first filter residue; (3) Then add water equal to 6 times the total weight of the first filter residue to the first filter residue and soak for 1 hour, then boil for 1 hour; after filtration and centrifugation, collect the second filtrate, and combine the first filtrate and the second filtrate; concentrate under reduced pressure at a temperature of 50℃ and a pressure of -0.09MPa to a thick paste with a relative density of 1.20; dry under reduced pressure at a temperature of 50℃ and a pressure of -0.1MPa to obtain the final product.

[0031] Example 3 A traditional Chinese medicine composition for treating Alzheimer's disease, the composition comprising the following raw materials in parts by weight: 28 parts Coptis chinensis, 25 parts Cinnamomum cassia, 22 parts Rhizoma Citri Reticulatae, 19 parts Acorus tatarinowii, 20 parts Polygala tenuifolia, 13 parts Rehmanniae Radix Praeparata, 12 parts Clematis chinensis, 11 parts Poria cocos, and 8 parts Glycyrrhiza uralensis.

[0032] A method for preparing a traditional Chinese medicine composition for treating Alzheimer's disease specifically includes the following steps: (1) Weigh out each ingredient according to the following weight proportions: 28 parts of Coptis chinensis, 25 parts of cinnamon, 22 parts of golden thread, 19 parts of Acorus tatarinowii, 20 parts of Polygala tenuifolia, 13 parts of Rehmannia glutinosa, 12 parts of Fragrant Vine, 11 parts of Poria cocos, and 8 parts of Glycyrrhiza uralensis. After pulverizing, pass through an 80-mesh sieve to obtain Chinese medicine powder for later use. (2) Soak the Chinese medicine powder prepared in step (1) in 12 times the total weight of the Chinese medicine powder in water for 3 hours, then decoct for 3 hours; filter, centrifuge, and collect the first filtrate and the first filter residue; (3) Then add 10 times the total weight of the first filter residue to the first filter residue and soak for 3 hours, then boil for 2 hours; after filtration and centrifugation, collect the second filtrate, and combine the first filtrate and the second filtrate; concentrate under reduced pressure at a temperature of 70℃ and a pressure of -0.09MPa to a thick paste with a relative density of 1.26; dry under reduced pressure at a temperature of 60℃ and a pressure of -0.1MPa to obtain the final product.

[0033] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the gold thread in a traditional Chinese medicine composition for treating Alzheimer's disease is omitted, while all other aspects are the same as in Example 1.

[0034] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the Chinese herbal medicine ingredient for treating Alzheimer's disease, *Tetrapanax papyriferus*, is omitted; otherwise, they are the same as in Example 1.

[0035] Experimental Example 1 The following are efficacy tests of a traditional Chinese medicine composition for treating Alzheimer's disease prepared in Examples 1-3 and Comparative Examples 1-2: (1) One hundred 3-month-old male 5XFAD mice were selected for the experiment and housed in separate cages in an SPF-grade animal laboratory. The ambient temperature was controlled at 22±2℃ and the relative humidity at 50-60%. The mice were allowed free access to food and water, with a 12-hour light-dark cycle. After 7 days of acclimatization, the 5XFAD mice were randomly divided into four groups: Example 1 high-dose group, Example 1 medium-dose group, Example 1 low-dose group, Example 2 low-dose group, Example 3 low-dose group, Comparative Example 1 low-dose group, Comparative Example 2 low-dose group, and model group, with 10 mice in each group. In addition, 10 3-month-old male wild-type mice were selected as the normal control group.

[0036] (2) Mice in each group were administered the corresponding traditional Chinese medicine composition by gavage at 10:00 AM every day for drug intervention: Example 1 High-dose group: Based on raw drug, 8g / kg of the traditional Chinese medicine composition of Example 1 was administered by gavage once a day for 3 consecutive months.

[0037] In Example 1, the dosage group was administered 4 g / kg of the traditional Chinese medicine composition of Example 1 by gavage once a day for 3 consecutive months.

[0038] Example 1 Low-dose group: 2g / kg of the traditional Chinese medicine composition of Example 1 was administered by gavage once a day for 3 consecutive months.

[0039] Example 2 Low-dose group: 2g / kg of the traditional Chinese medicine composition of Example 2 was administered by gavage once a day for 3 consecutive months.

[0040] Example 3 Low-dose group: Based on raw drug, 2g / kg of the traditional Chinese medicine composition of Example 3 was administered by gavage once a day for 3 consecutive months.

[0041] Comparative Example 1 Low-dose group: Based on crude drug, 2 g / kg of the traditional Chinese medicine composition of Comparative Example 1 was administered by gavage once a day for 3 consecutive months.

[0042] Comparative Example 2 Low-dose group: Based on crude drug, 2 g / kg of the traditional Chinese medicine composition of Comparative Example 2 was administered by gavage once a day for 3 consecutive months.

[0043] Model group: The same volume of physiological saline was administered by gavage once a day for 3 consecutive months.

[0044] Normal control group: The same volume of normal saline was administered by gavage once a day for 3 consecutive months.

[0045] (3) After 3 months of gavage treatment, the spatial learning and memory abilities of mice in each group were assessed using the MWM test. The specific experimental steps were as follows: The circular pool was divided into four quadrants. A hidden platform was placed at the center of the diagonal of the fourth quadrant, filled with water to a depth of about 20 cm, and milk powder was added and stirred until opaque. The hidden platform was fixed at the center of the fourth quadrant, 1 cm below the water surface. The positioning and navigation experiment was conducted for 5 consecutive days, with 4 training sessions per day (30-minute intervals). Mice were placed in the water facing the pool wall from the first, second, and third quadrants, respectively, and the time it took to find the platform within 60 seconds (i.e., the escape latency period) was recorded. If the mouse did not find the platform within 60 seconds, the experimenter guided it to the platform and kept it there for 15 seconds. After the positioning and navigation were completed, the platform was removed and a space exploration experiment was conducted: mice were placed in the water at the same entry point in the second quadrant, and the time spent by the mice in the target quadrant (the fourth quadrant where the original platform was located) and the distance traveled in the target quadrant were recorded within 60 seconds. The percentage of time spent by the mice in the target quadrant and the percentage of distance traveled by the mice in the target quadrant were calculated. The results are shown in Table 1.

[0046] Table 1. Effects of different traditional Chinese medicine compositions on spatial learning and memory abilities in mice. The results are shown in Table 1, which illustrates the effects of different traditional Chinese medicine (TCM) compositions on the spatial learning and memory abilities of mice. As shown in Table 1, compared with the model group, the low-dose group of Comparative Example 1, and the low-dose group of Comparative Example 2, the TCM compositions for treating Alzheimer's disease prepared in Examples 1-3 of this invention significantly increased the time spent in the target quadrant and the distance traveled. This indicates that the TCM compositions of this invention can significantly improve cognitive dysfunction in 5XFAD mice and enhance their spatial learning and memory abilities.

[0047] (4) After completing the MWM test, all experimental mice were anesthetized and their brains were quickly removed, frozen sections were prepared, and immunohistochemistry was used to detect the deposition of hippocampal Aβ plaques in the brain tissue of each group of mice. ImageJ was used to quantitatively analyze the plaque area ratio in order to explore the effect of the traditional Chinese medicine composition on the pathological changes of AD. The results are shown in Table 2.

[0048] Table 2. Effects of different traditional Chinese medicine compositions on Aβ plaque area The results are shown in Table 2, which illustrates the effect of different traditional Chinese medicine (TCM) compositions on the area of ​​Aβ plaques. As can be seen from Table 2, compared with the model group, the low-dose group of Comparative Example 1, and the low-dose group of Comparative Example 2, the TCM compositions for treating Alzheimer's disease prepared in Examples 1-3 of this invention can significantly reduce the area of ​​dentate gyrus plaques. This indicates that the TCM compositions of this invention can effectively inhibit the amyloid degradation pathway of APP and significantly reduce the area of ​​Aβ plaques deposited in the dentate gyrus of 5XFAD mice, thereby exerting a therapeutic effect on Alzheimer's disease.

[0049] (5) To further investigate the mechanism of action of the herbal composition of the present invention in reducing amyloid plaque deposition, GAPDH was used as an internal reference gene, and the expression levels of PSEN1, BACE1 and C83 / APP related proteins in mouse hippocampus were detected by Western blotting. The results are as follows: Figure 1 , Figure 2 , Figure 3 As shown.

[0050] The results are as follows Figure 1 The image shows the effect of different traditional Chinese medicine compositions used to treat Alzheimer's disease on the protein expression level of PSEN1. Figure 1 It can be seen that, compared with the model group, the low-dose group of Comparative Example 1, and the low-dose group of Comparative Example 2, the traditional Chinese medicine composition for treating Alzheimer's disease prepared in Examples 1-3 of this invention can significantly reduce the protein expression level of PSEN1 in the hippocampus of 5XFAD mice.

[0051] The results are as follows Figure 2 The image shows the effect of different traditional Chinese medicine compositions used to treat Alzheimer's disease on the protein expression level of BACE1. Figure 2It can be seen that, compared with the model group, the low-dose group of Comparative Example 1, and the low-dose group of Comparative Example 2, the traditional Chinese medicine composition for treating Alzheimer's disease prepared in Examples 1-3 of this invention can significantly reduce the protein expression level of BACE1 in the hippocampus of 5XFAD mice.

[0052] The results are as follows Figure 3 The image shows the effect of different traditional Chinese medicine compositions used to treat Alzheimer's disease on the protein expression level of C83 / APP. Figure 3 It can be seen that, compared with the model group, the low-dose group of Comparative Example 1, and the low-dose group of Comparative Example 2, the traditional Chinese medicine composition for treating Alzheimer's disease prepared in Examples 1-3 of this invention can significantly increase the protein expression level of C83 / APP in the hippocampus of 5XFAD mice.

[0053] In summary, the traditional Chinese medicine compositions for treating Alzheimer's disease prepared in Examples 1-3 of this invention can significantly reduce the protein expression levels of BACE1 and PSEN1 in the hippocampus of 5XFAD mice and significantly upregulate the expression level of C83 / APP. This indicates that the traditional Chinese medicine compositions of this invention exert their therapeutic effect on Alzheimer's disease by inhibiting the amyloid degradation pathway of APP (reducing the expression of BACE1 and PSEN1) and promoting the non-amyloid degradation pathway (increasing the production of C83 / APP), thereby reducing Aβ production and plaque deposition.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. Based on the present invention, some modifications or substitutions can be made, but these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of protection claimed by the present invention.

Claims

1. A traditional Chinese medicine composition for treating senile dementia, characterized in that, The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 23-28 parts of Coptis chinensis, 20-25 parts of Cinnamomum cassia, 18-22 parts of Rhizoma Citri Reticulatae, 13-19 parts of Acorus tatarinowii, 15-20 parts of Radix Polygalae, 9-13 parts of Radix Rehmanniae, 6-12 parts of Rhizoma Citri Reticulatae, 7-11 parts of Poria cocos, and 3-8 parts of Radix Glycyrrhizae.

2. The traditional Chinese medicine composition for treating senile dementia according to claim 1, characterized in that, The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 25 parts Coptis chinensis, 22 parts Cinnamomum cassia, 20 parts Rhizoma Citri Reticulatae, 16 parts Acorus tatarinowii, 17 parts Polygala tenuifolia, 11 parts Rehmanniae Radix Praeparata, 9 parts Stellariae Aristolochiae, 9 parts Poria cocos, and 5 parts Glycyrrhiza uralensis.

3. A method for preparing a traditional Chinese medicine composition for treating senile dementia according to claim 1 or 2, characterized in that, Specifically, the following steps are included: (1) Weigh out each ingredient according to the weight proportions: Coptis chinensis, cinnamon, golden thread, Acorus tatarinowii, Polygala tenuifolia, Rehmannia glutinosa, Fragrant Vine, Poria cocos, and Glycyrrhiza uralensis. Grind them into powder and sieve them to obtain Chinese medicine powder for later use. (2) Soak the Chinese herbal powder prepared in step (1) in water, and then decoct it; Filter, centrifuge, and collect the first filtrate and the first filter residue; (3) Then add water to the first filter residue to soak it, and then boil it; After filtration and centrifugation, collect the second filtrate, combine the first and second filtrates, concentrate, and dry to obtain the final product.

4. The method for preparing a traditional Chinese medicine composition for treating senile dementia according to claim 3, characterized in that, The mesh size of the sieve in step (1) is 60-80.

5. A method for preparing a traditional Chinese medicine composition for treating senile dementia according to claim 3, characterized in that, In step (2), the amount of water added is 8-12 times the total weight of the Chinese herbal powder; the soaking time is 1-3 hours; and the decoction time is 2-3 hours.

6. A method for preparing a traditional Chinese medicine composition for treating senile dementia according to claim 3, characterized in that, In step (3), the amount of water added is 6-10 times the total weight of the first filter residue; the soaking time is 1-3 hours; and the decocting time is 1-2 hours.

7. A method for preparing a traditional Chinese medicine composition for treating senile dementia according to claim 3, characterized in that, In step (3), the concentration temperature is 50-70℃, and the concentration is carried out to a thick paste with a relative density of 1.20-1.

26.

8. A method for preparing a traditional Chinese medicine composition for treating senile dementia according to claim 3, characterized in that, The drying temperature in step (3) is 50-60℃.

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