Silver-palladium alloy nanowire and preparation method and application thereof

Abstract

The invention discloses a silver-palladium alloy nanowire and a preparation method and application thereof, and belongs to the technical field of nano alloy materials. The preparation method comprises the following steps: (1) adding an aqueous solution of hexadecyl trimethyl ammonium bromide and an aqueous solution of dopamine hydrochloride into an aqueous solution of silver nanowires, uniformly stirring, dropwise adding an aqueous solution of sodium chloropalladate, and continuously stirring until the reaction is completed to obtain a reaction product; and (2) carrying out centrifugal washing on the reaction product to obtain the silver-palladium alloy nanowire. The prepared silver-palladium alloy nanowire material is used for catalytic reduction of 4-nitrophenol, verification of a photothermal effect and glutathione level detection, so that the application of the silver-palladium alloy nanowire material in the field of catalysis is effectively expanded; and a brand new nano-alloy material and an application approach are provided for high-sensitivity and high-selectivity detection of glutathione level due to the exertion of a photothermal effect.

Description

Technical Field

[0001] This invention belongs to the field of nano-alloy materials technology, and relates to a silver-palladium alloy nanowire, its preparation method and application. Background Technology

[0002] Silver-based alloys possess excellent electrical conductivity, corrosion resistance, high-temperature stability, and oxidation resistance. They are widely used as conductive materials, catalysts, and raw materials for structural components in electronic device manufacturing, chemical industry, and aerospace. However, despite their wide range of applications, silver-based alloys have not yet fully realized their potential.

[0003] 4-Nitrophenol (4-NP), an important chemical intermediate and a typical nitro aromatic compound, is widely found in wastewater from the dye, pesticide, pharmaceutical, and explosive industries. Research on the catalytic degradation of 4-NP could reduce the impact of these toxic pollutants on aquatic organisms and mitigate their effects on human health through the food chain.

[0004] The photothermal effects of inorganic nanomaterials have been studied relatively early and are quite mature. Typical silver-based nanomaterials often suffer from difficulties in designing uniform structures, poor biocompatibility, and poor performance control. Researching novel silver-based nanostructures can broaden the application scope of such nanostructures.

[0005] Glutathione (GSH) is an important antioxidant found in human cells, helping to protect against free radical damage. It plays a key role in maintaining cell health, supporting the immune system, and detoxification. Detecting glutathione levels can assess the body's antioxidant capacity and overall health, making the detection of glutathione levels of great significance.

[0006] Therefore, it is necessary to provide a silver-palladium alloy nanowire, its preparation method and application, to expand the application field of silver-palladium alloy nanowires and give fuller play to the properties and functions of silver-palladium alloy nanowire materials. Summary of the Invention

[0007] To overcome the problems in the prior art, this invention uses the prepared silver-palladium alloy nanowire material in heterogeneous catalytic hydrogenation reduction of 4-nitrophenol, analysis of photothermal effects, and detection of glutathione levels, thereby achieving high selectivity and high sensitivity detection of glutathione levels.

[0008] To achieve the above objectives, the present invention is implemented through the following technical solution: This invention provides a method for preparing silver-palladium alloy nanowires, the method comprising: (1) Add the aqueous solution of hexadecyltrimethylammonium bromide (CTAB) and the aqueous solution of dopamine hydrochloride to the aqueous solution of silver nanowires, stir evenly, and then add the aqueous solution of sodium chloropalladium (Na2PdCl4) dropwise. Continue stirring until the reaction is complete to obtain the reaction product.

[0009] (2) The reaction product was centrifuged and washed to obtain silver-palladium alloy nanowires (AgPd NWs).

[0010] Preferably, in step (1), the molar ratio of hexadecyltrimethylammonium bromide to silver nanowires is hexadecyltrimethylammonium bromide : silver nanowires = (0.1 × 10⁻⁶) / (1 + ... 3 ~10×10 3 The mass ratio of dopamine hydrochloride to silver nanowires is (10~100):1, and the molar ratio of sodium chloropalladium to silver nanowires is (0.005~0.1):1.

[0011] In the aqueous solution of CTAB, the amount of water used is only sufficient to dissolve all of CTAB; in the aqueous solution of dopamine hydrochloride, the amount of water used is only sufficient to dissolve all of dopamine hydrochloride; in the aqueous solution of sodium chloropalladium, the amount of water used is only sufficient to dissolve all of sodium chloropalladium; and in the aqueous solution of silver nanowires, the amount of water used is sufficient to uniformly dissolve and disperse the silver nanowires.

[0012] Preferably, in step (1), the method for preparing silver nanowires includes the following steps: S1: Dissolve polyvinylpyrrolidone (PVP), FeCl3, and AgNO3 in ethylene glycol to obtain polyvinylpyrrolidone solution, FeCl3 solution, and AgNO3 solution, respectively. S2: The polyvinylpyrrolidone solution was heated, and then FeCl3 solution and AgNO3 solution were added to the polyvinylpyrrolidone solution. After the reaction was completed, silver nanowires were obtained.

[0013] In the PVP solution, the amount of ethylene glycol used should be sufficient to completely dissolve the PVP; in the FeCl3 solution, the amount of ethylene glycol used should be sufficient to completely dissolve the FeCl3; and in the AgNO3 solution, the amount of ethylene glycol used should be sufficient to completely dissolve the AgNO3.

[0014] Preferably, the K value of the polyvinylpyrrolidone is 60 to 120.

[0015] Preferably, in step S2, the mass ratio of polyvinylpyrrolidone to AgNO3 is polyvinylpyrrolidone:AgNO3 = (0.45~2):1, and the mass ratio of FeCl3 to AgNO3 is FeCl3:AgNO3 = (0.00054~0.003):1.

[0016] Preferably, in step S2, the polyvinylpyrrolidone solution is heated in an oil bath at a temperature of 120~160℃ for 25 minutes. While maintaining the heating, FeCl3 solution and AgNO3 solution are added to the polyvinylpyrrolidone solution.

[0017] In another aspect, the present invention provides silver-palladium alloy nanowires prepared by the above preparation method, wherein the surface of the silver-palladium alloy nanowires has a rough structure.

[0018] This invention also proposes the application of the above-mentioned silver-palladium alloy nanowires in the analysis of heterogeneous catalytic hydrogenation reduction of 4-nitrophenol, the photothermal effect generated after irradiation with an 808 laser, and the detection of glutathione content using ultraviolet-visible spectroscopy.

[0019] The beneficial effects of this invention are: The silver-palladium alloy nanowire material prepared by this invention provides a new candidate material for inorganic photothermal conversion materials. It effectively expands the application field of silver-palladium alloy nanowire materials in the detection of glutathione levels. At the same time, it provides a brand-new material for heterogeneous catalytic hydrogenation reduction of 4-nitrophenol, photothermal conversion analysis, and high-sensitivity and high-selectivity detection of glutathione levels. Attached Figure Description

[0020] Figure 1 The image shows the XRD pattern of the silver-palladium alloy nanowires prepared in Example 1 of this invention. Figure 2 The image shows the UV-vis spectrum of the silver-palladium alloy nanowires prepared in Example 1 of this invention. Figure 3 The images show the SEM, TEM, and elemental distribution diagrams of the silver-palladium alloy nanowires prepared in Example 1 of this invention. (a) is the SEM image of the silver-palladium alloy nanowires, (b) is the TEM image of the silver-palladium alloy nanowires, (c) is the STEM image of a single silver-palladium alloy nanowire, (d) is the mapping diagram of silver (Ag) in a single silver-palladium alloy nanowire, (e) is the mapping diagram of palladium (Pd) in a single silver-palladium alloy nanowire, and (f) is the mapping combination diagram of silver and palladium in a single silver-palladium alloy nanowire. Figure 4The following are images showing the effect of the silver-palladium alloy nanowires prepared in Example 1 of this invention on the heterogeneous catalytic reduction of 4-nitrophenol. (a) UV-vis of Ag NWs on the hydrogenation reduction of tetranitrophenol; (b) UV-vis of Pd NWs on the hydrogenation reduction of tetranitrophenol; (c) UV-vis of AgPd NWs on the hydrogenation reduction of tetranitrophenol; (d) Comparison of the reaction rate constants of Ag NWs, PdNWs and AgPdNWs on the catalytic reduction of 4-NP.

[0021] Figure 5 The graph shows the temperature rise of the silver-palladium alloy nanowires prepared in this invention after being dissolved in solutions of different pH values ​​and irradiated with an 808nm laser.

[0022] Figure 6 The graphs show the performance of the silver-palladium alloy nanowires prepared in Example 1 of this invention in detecting GSH. Among them, (a) is the UV-Vis absorption spectrum as the GSH concentration changes, (b) is the linear calibration curve for measuring different concentrations of glutathione (GSH), (c) is the effect of different amino acids and interfering ions on the maximum absorption peak of the silver-palladium alloy nanowire-TMB mixed solution without the addition of GSH, and (d) is the change of the maximum absorption peak after the addition of GSH and other amino acids and interfering ions to the silver-palladium alloy nanowire-TMB mixed solution. Detailed Implementation

[0023] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, but the scope of protection of the present invention is not limited to the content described. Example 1

[0024] This embodiment prepares silver-palladium alloy nanowires using the following method: (1) Preparation of silver nanowires: PVP (K value: 90) ethylene glycol solution was poured into a round-bottom flask and heated in an oil bath at 140℃ for 25 min. Then FeCl3 ethylene glycol solution and AgNO3 ethylene glycol solution were added sequentially, where PVP:AgNO3=0.9:1; FeCl3:AgNO3=0.00135:1, and the reaction was carried out for 50 min. The entire reaction process was carried out at a speed of 400 r / min (the color of the solution changed from light yellow to light white, and finally to milky white, proving that the silver nanowires were successfully prepared).

[0025] (2) Preparation of silver-palladium alloy nanowires: CTAB solution and dopamine hydrochloride solution were added to AgNWs aqueous solution and stirred evenly at 300 rpm. Subsequently, Na2PdCl4 solution was added dropwise and stirring was continued for 20 min. The ratio of CTAB to silver nanowires was 5 × 10⁻⁶. 3The ratio of dopamine hydrochloride to silver nanowires was 60:1; the ratio of Na₂PdCl₄ to silver nanowires was 0.0275:1. It can be observed that the solution color gradually changes from gray to brown, and finally to black, indicating that the reaction is complete.

[0026] (3) The product was centrifuged at 5000 rpm for 5 minutes. This process was repeated five times to thoroughly wash away excess surfactant and unreacted material, and silver-palladium alloy nanowires (AgPd NWs) were obtained.

[0027] X-ray diffraction experiments were performed on the AgPd NWs prepared in this embodiment, and the results are as follows: Figure 1 As shown.

[0028] pass Figure 1 It can be seen that the silver-palladium alloy nanowires prepared by this invention are not significantly different from the standard PDF card (04-0783) of the XRD pattern of silver nanowires and the standard PDF card (46-1043) of palladium nanowires. Moreover, the main diffraction peaks of the silver-palladium alloy nanowires are all located between the silver nanowires and the palladium nanowires, indicating that the silver-palladium alloy nanowires prepared by this invention have good crystallinity and the silver-palladium alloy nanostructure has been successfully prepared.

[0029] The AgPd NWs prepared in this embodiment were subjected to UV-Vis spectroscopy experiments, and the results are as follows: Figure 2 As shown.

[0030] pass Figure 2 It can be seen that the silver-palladium alloy nanowires prepared by this invention exhibit a wide resonant absorption characteristic. Since the main component of living organisms is water, biological tissues absorb and scatter light minimally in the range of 750nm to 900nm, allowing light to penetrate deeper within this wavelength range. An 808nm laser precisely falls within the central region of this biological tissue's "optical therapy window," making 808nm a widely used photothermal wavelength band. Figure 2 As can be seen, the silver-palladium alloy nanowires still exhibit strong absorption properties at this location.

[0031] The AgPd NWs prepared in this embodiment were subjected to SEM, TEM, and elemental distribution analysis experiments, and the results are as follows: Figure 3 As shown.

[0032] pass Figure 3 It can be seen that the AgPd NWs prepared in this invention are mainly composed of silver and palladium elements, which are uniformly distributed and show a high degree of overlap. This indicates that the two elements coexist uniformly in the nanowires without forming independent phase-separated structures, thus confirming the successful preparation of silver-palladium alloy nanowires.

[0033] Although centrifugation and washing of the reaction products during the preparation process can remove excess surfactants and unreacted reagents, CTAB molecules may still be coated on the surface of AgPd NWs. CTAB molecules have strong biotoxicity. Therefore, to ensure the accuracy of subsequent experimental results and the biocompatibility of AgPd NWs in biological applications, the AgPd NWs prepared in this example were redispersed and dissolved in ultrapure water with 1 ml of 25 mmol / L dithiol polyethylene glycol added. The solution was stirred at 200 rpm for 30 minutes to ensure homogenization. The solution was then stored at 4°C overnight to obtain surface-thiol-modified AgPd NWs, thus eliminating the influence of CTAB on subsequent experimental results. The treated AgPd NWs were then used for photothermal experiments and GSH detection experiments.

[0034] Example of effect 1 First, 10 mM of 4-NP was placed in a 1 cm thick quartz cuvette, and 0.8 mL of a 0.1 mol / L NaBH4 solution was added. The solution turned pale yellow, indicating a 4-NP to NaBH4 concentration ratio of 1:200. Then, a certain amount of AgPd NWs solution was added to initiate the catalytic reaction. The changes in the UV-Vis absorption peaks within the 250 nm-500 nm range were measured, and the catalytic effect of the AgPd NWs nanocatalyst was evaluated and analyzed based on the change in the maximum absorption peak over time. Figure 4 It can be clearly seen that the catalytic activity of palladium nanowires and silver nanowires is not as high as that of silver-palladium alloy nanowires. Figure 4 ab). However, palladium nanowires have significant advantages over silver nanowires because palladium itself possesses excellent hydrogenation catalytic activity, effectively activating hydrogen molecules and facilitating the hydrogenation reaction. Furthermore, coating silver nanowires with palladium promotes the modulation of the electronic properties of the palladium layer on the surface, thereby further regulating and optimizing the electronic structure of the AgPd NWs alloy, enhancing its catalytic activity, and accelerating the reaction rate. Figure 4 c). Figure 4 Figure d shows the calculated comparison of the catalytic effects of the three nanocatalysts. The catalytic reaction rate constant of 20 μL AgPd NWs is K. nor =1.065×10 3 min -1 ·mmol -1 ; 50 uL of Ag NWs K nor = 25.2 min -1 ·mmol -1 ,; 50uL of Pd NWs K nor =130.6 min -1·mmol -1 These results strongly suggest that this comparison demonstrates the superior catalytic activity of silver-palladium alloy nanowires.

[0035] Example 2 Using an 808nm laser (1.5W / cm) 2 The AgPd NWs were irradiated with PBS solutions of different pH values ​​for 20 minutes, and the results are as follows: Figure 5 As shown, under pH conditions of 6.3, 7.3, and 8.3, the PBS solutions with added AgPd NWs all exhibited significant temperature increases, reaching maximum temperatures of 48.2°C, 52.5°C, and 52.1°C, respectively. In contrast, the control group, which contained only ultrapure water and no AgPd NWs, showed a maximum temperature of only 33.8°C after laser irradiation. Figure 5 a) This indicates that AgPd NWs exhibit excellent photothermal conversion effects under near-physiological environmental conditions. Based on widespread understanding and practical experience, cancer cells are generally damaged after being maintained at 42°C for 3 minutes. This suggests that under laser irradiation, AgPd NWs possess sufficient heating effect to kill cancer cells. Figure 5 (b) shows the temperature rise of AgPd NWs under different laser powers. The temperature rise becomes more significant with increasing laser power, reaching 2 W / cm². 2 The highest temperature reached under high power irradiation was 68.1°C. This demonstrates that the photothermal effect of AgPd NWs is highly controllable. In practical applications, the local temperature can be precisely controlled by adjusting the laser power to achieve the best therapeutic effect and avoid excessive damage to normal tissues. Figure 5 (c) This indicates that AgPd NWs can still heat up to above 50°C after three cycles of repeated irradiation with a 1.5 W / cm² laser, demonstrating good photothermal stability. These photothermal effect test results show that AgPd NWs are not prone to structural damage or significant performance degradation due to laser irradiation. The good photothermal stability and reusability exhibited by AgPd NWs provide an important foundation for their application in cancer cell killing, biomedicine, and clinical medicine. Figure 5 (d) is a schematic diagram of infrared thermography of AgPd NWs solution at different time points, which can intuitively reflect the photothermal heating process of AgPd NWs solution. These images vividly demonstrate that AgPd NWs can efficiently convert near-infrared light energy into heat energy and the heat field is uniformly distributed, providing intuitive evidence for its ability to achieve localized and precise heating in photothermal therapy.

[0036] Example 3 900 μL of TMB solution (0.6 mM), 300 μL of HAc-NaAc buffer solution (pH=4), 100 μL of H2O2 solution (0.3 M), and 50 μL of silver-palladium alloy nanowires were reacted at 40 °C for 50 min. Then, 200 μL of GSH solutions of different concentrations were added, and after incubation for 2 min, the absorbance of the solutions was measured by UV-Vis spectroscopy. The absorbance was used to evaluate the detection ability of the silver-palladium alloy nanowires for GSH. Furthermore, to evaluate the selectivity of the silver-palladium alloy nanowires for GSH detection, 1 mM of different types of amino acid ions were used as controls, and their absorbance at 652 nm was compared, and ΔA was measured.

[0037] pass Figure 6 As shown in (a) and (b), the absorbance value at 652 nm decreases linearly with increasing GSH concentration. It is evident that the solution color changes from deep blue to light blue, or even nearly colorless, after adding different amounts of GSH. This significant color change, visible to the naked eye, makes rapid on-site detection of GSH possible. The AgPd NWs of this invention have a detection range of 0-1000 μM for GSH, the widest detection range reported to date. The relationship between the absorbance difference ΔA at 652 nm and the GSH concentration satisfies the linear regression equation y = 0.00171x - 0.04035, while the fit index R of the AgPd NWs for GSH detection using this invention is... 2 =0.995. The closer the fitting index is to 1, the higher the detection accuracy, indicating that the silver-palladium alloy nanowires of the present invention have extremely high accuracy in GSH detection.

[0038] pass Figure 6 (c) and (d) show that the addition of other amino acids and inorganic ion solutions that do not contain GSH has almost no effect on ox-TMB, and the change in ΔA is very small (e.g., Figure 6 (c) shows that adding GSH solution containing other amino acids and inorganic ions can cause a significant color change in the ox-TMB solution, with a large change in ΔA (e.g., Figure 6 (d) This shows that the influence of different interferences on the detection of GSH is negligible, proving that the AgPd NWs of this invention have excellent selectivity for the detection of GSH. Example 2

[0039] This embodiment prepares silver-palladium alloy nanowires using the following method: (1) Preparation of silver nanowires: PVP (K value: 60) in ethylene glycol was poured into a round-bottom flask and heated in an oil bath at 120℃ for 25 min. Then, FeCl3 in ethylene glycol and AgNO3 in ethylene glycol were added sequentially, where PVP:AgNO3 = 0.45:1; FeCl3:AgNO3 = 0.00054:1. The reaction was carried out for 50 min at a rotation speed of 400 r / min (the color of the solution changed from pale yellow to pale white, and finally to milky white, proving that the silver nanowires were successfully prepared).

[0040] (2) Preparation of silver-palladium alloy nanowires: CTAB solution and dopamine hydrochloride solution were added to AgNWs aqueous solution and stirred evenly at 300 rpm. Subsequently, Na2PdCl4 solution was added dropwise and stirring was continued for 20 min. The ratio of CTAB to silver nanowires was 0.1 × 10⁻⁶. 3 The ratio of dopamine hydrochloride to silver nanowires was 10:1; the ratio of Na₂PdCl₄ to silver nanowires was 0.005:1. It can be observed that the solution color gradually changes from gray to brown, and finally to black, indicating that the reaction is complete.

[0041] (3) The product was centrifuged at 5000 rpm for 5 minutes. This process was repeated five times to thoroughly wash away excess surfactant and unreacted material, and silver-palladium alloy nanowires (AgPd NWs) were obtained.

[0042] The performance of the AgPd NWs prepared in this embodiment is similar to that of the AgPd NWs in Example 1. Example 3

[0043] This embodiment prepares silver-palladium alloy nanowires using the following method: (1) Preparation of silver nanowires: PVP (K value: 120) in ethylene glycol was poured into a round-bottom flask and heated in an oil bath at 160℃ for 25 min. Then, FeCl3 in ethylene glycol and AgNO3 in ethylene glycol were added sequentially, where PVP:AgNO3 = 2:1; FeCl3:AgNO3 = 0.003:1. The reaction was carried out for 50 min at a rotation speed of 400 r / min (the color of the solution changed from pale yellow to pale white, and finally to milky white, proving that the silver nanowires were successfully prepared).

[0044] (2) Preparation of silver-palladium alloy nanowires: CTAB solution and dopamine hydrochloride solution were added to AgNWs aqueous solution and stirred evenly at 300 rpm. Subsequently, Na2PdCl4 solution was added dropwise, and stirring was continued for 20 min. The ratio of CTAB to silver nanowires was 10 × 10⁻⁶. 3The ratio of dopamine hydrochloride to silver nanowires was 100:1; the ratio of Na₂PdCl₄ to silver nanowires was 0.1:1. It can be observed that the solution color gradually changes from gray to brown, and finally to black, indicating that the reaction is complete.

[0045] (3) The product was centrifuged at 5000 rpm for 5 minutes. This process was repeated five times to thoroughly wash away excess surfactant and unreacted material, and silver-palladium alloy nanowires (AgPd NWs) were obtained.

[0046] The performance of the AgPd NWs prepared in this embodiment is similar to that of the AgPd NWs in Example 1.

[0047] In summary, this invention is the first to use the prepared silver-palladium alloy nanowires for heterogeneous catalytic hydrogenation reduction of 4-nitrophenol, achieving photothermal conversion, and detecting glutathione levels. This not only enriches the catalytic applications of silver-palladium alloys but also effectively expands the application fields of silver-palladium alloy nanowire materials. At the same time, it provides a new approach and candidate materials for the catalytic reduction of 4-NP, inorganic photothermal conversion, and high-sensitivity, high-selectivity detection of glutathione levels.

[0048] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for preparing silver-palladium alloy nanowires, characterized in that: The preparation method includes: (1) Add the aqueous solution of hexadecyltrimethylammonium bromide and the aqueous solution of dopamine hydrochloride to the aqueous solution of silver nanowires, stir evenly, add the aqueous solution of sodium chloropalladium dropwise, and continue stirring until the reaction is complete to obtain the reaction product; (2) The reaction product was centrifuged and washed to obtain silver-palladium alloy nanowires.

2. The preparation method according to claim 1, characterized in that: In step (1), the molar ratio of hexadecyltrimethylammonium bromide to silver nanowires is hexadecyltrimethylammonium bromide : silver nanowires = (0.1 × 10⁻⁶) / ( ... 3 ~10×10 3 The mass ratio of dopamine hydrochloride to silver nanowires is (10~100):1, and the molar ratio of sodium chloropalladium to silver nanowires is (0.005~0.1):

1.

3. The preparation method according to claim 1, characterized in that: In step (1), the preparation method of silver nanowires includes the following steps: S1: Dissolve polyvinylpyrrolidone, FeCl3, and AgNO3 in ethylene glycol to obtain polyvinylpyrrolidone solution, FeCl3 solution, and AgNO3 solution, respectively. S2: The polyvinylpyrrolidone solution was heated, and then FeCl3 solution and AgNO3 solution were added to the polyvinylpyrrolidone solution. After the reaction was completed, silver nanowires were obtained.

4. The preparation method according to claim 3, characterized in that: The K value of the polyvinylpyrrolidone is 60~120.

5. The preparation method according to claim 3, characterized in that: In step S2, the mass ratio of polyvinylpyrrolidone to AgNO3 is polyvinylpyrrolidone:AgNO3 = (0.45~2):1, and the mass ratio of FeCl3 to AgNO3 is FeCl3:AgNO3 = (0.00054~0.003):

1.

6. The preparation method according to claim 3, characterized in that: In step S2, the polyvinylpyrrolidone solution is heated in an oil bath at a temperature of 120-160°C for 25 minutes. While maintaining the heating, FeCl3 solution and AgNO3 solution are added to the polyvinylpyrrolidone solution.

7. The silver-palladium alloy nanowires prepared by the preparation method according to any one of claims 1-6, characterized in that: The silver-palladium alloy nanowires have a rough surface structure.

8. The application of the silver-palladium alloy nanowires according to claim 7 in the heterogeneous catalytic hydrogenation reduction of 4-nitrophenol.

9. The application of the silver-palladium alloy nanowires according to claim 7 in the detection of glutathione content using ultraviolet-visible spectroscopy.

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