Integrated extraction and purification instrument for myxomycete nucleic acid and giant salamander peptide magnetic beads

Abstract

Belonging to the technical field of purifiers, the invention relates to a myxomycete nucleic acid and giant salamander peptide magnetic bead integrated extraction and purification instrument, which comprises a purification instrument main body, an electric pump, a fixed pipe, a storage cylinder, a power supply control cabinet, a connection pipe, a protection mechanism, a fixing mechanism and a damping mechanism. A crushing blade and an inner sawtooth ring are arranged in a crushing pipe of the electric pump and are matched with a transmission shaft to drive to efficiently crush materials, and an activated carbon filtration pore plate synchronously completes filtration and self-cleaning; the protection mechanism drives a swing rod through a servo motor to link lifting of a moving wheel and opening and closing of a protection plate, and synchronous protection of an opening of a fixed pipe is achieved when equipment moves. The fixing mechanism controls a clamping block through a servo motor, and the fixing pipe and the connecting pipe are clamped and fixed in an auxiliary mode. Integrated extraction and purification of myxomycete nucleic acid and giant salamander peptide magnetic beads are achieved, crushing is sufficient, protection is reliable, connection is firm, damping adaptability is high, the extraction efficiency and purification precision are effectively improved, and the method is suitable for large-scale production scenes.

Description

Technical Field

[0001] This invention relates to the field of purification instrument technology, and more specifically, to an integrated extraction and purification instrument for *Saccharum spp.* nucleic acid and *Andrias davidianus* peptides using magnetic beads. Background Technology

[0002] As important bioactive substances in the fields of biomedicine and bioengineering, the efficiency and purity of the extraction and purification of *Ulva lactuca* nucleic acid and *Andrias davidianus* peptide magnetic beads directly affect the subsequent research and application results. Therefore, stringent requirements are placed on the performance of dedicated extraction and purification equipment. However, current technologies for the extraction and purification of *Ulva lactuca* nucleic acid and *Andrias davidianus* peptide magnetic beads mostly rely on a combination of decentralized equipment, lacking an integrated solution. This results in a cumbersome overall operation process and poor coordination between various steps, severely restricting the overall efficiency of extraction and purification.

[0003] Specifically, existing technologies have many prominent shortcomings:

[0004] (1) The material crushing effect is not good. The existing equipment has a simple crushing mechanism design and mostly adopts a single blade crushing mode, which makes it difficult to achieve uniform and sufficient crushing of materials. The large pieces of residual material will directly affect the purity of subsequent separation and extraction of nucleic acid and peptide magnetic beads.

[0005] (2) Lack of protection mechanism. During the movement of the equipment, the opening at the end of the fixed pipe lacks a synchronous linkage protection structure, which is easily contaminated by external dust and impurities. At the same time, the exposed interface also increases the risk of collision damage.

[0006] (3) Insufficient connection stability. Fixed pipes and connecting pipes are mostly fixed by flanges alone. During long-term operation, they are prone to loosening and leakage due to factors such as equipment vibration and material conveying pressure, which affects the continuity of operation.

[0007] (4) Poor shock absorption performance adaptability. The existing equipment shock absorption structure is mostly simple spring shock absorption, which cannot effectively buffer the high-frequency vibration generated during operation. This will not only reduce the accuracy of extraction and purification, but may also damage internal precision components. Moreover, the shock absorption effect cannot be flexibly adjusted according to the actual working conditions. Summary of the Invention

[0008] To address the problems mentioned in the background, this invention provides an integrated extraction and purification instrument for *Ulva lactuca* nucleic acid and *Andrias davidianus* peptides using magnetic beads, thereby resolving the issues raised in the background.

[0009] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows:

[0010] The integrated extraction and purification instrument for Tai Sui nucleic acid and giant salamander peptide magnetic beads includes a purification instrument body, an electric pump connected to the outer wall of the purification instrument body, and a fixed tube connected to the electric pump. An installation block is fixedly connected to the outer wall of the purification instrument body, a storage cylinder is provided on the outer wall of the purification instrument body, and a power control cabinet is provided on the outer wall of the purification instrument body. The end of the fixed tube is detachably connected to a connecting tube through a flange.

[0011] The electric pump includes:

[0012] A turbine cover is connected to one end of an electric pump. One end of the turbine cover is connected to an inlet, and the outer wall of the turbine cover is connected to an outlet. The output end of the electric pump is connected to a drive shaft. A turbine is installed inside the turbine cover. The drive shaft is fixedly connected to the turbine. One end of the inlet is connected to a flange, and one end of the flange is fixed to a crushing pipe.

[0013] Preferably, the crushing tube is provided with a plurality of second internal threaded sleeves, which are threadedly engaged with the drive shaft. A plurality of crushing blades are fixed on the outer wall of the second internal threaded sleeves. An inner serrated ring is threadedly connected to the inner wall of the crushing tube, and a plurality of serrated blades are fixed on the inner wall of the inner serrated ring.

[0014] An activated carbon filter plate is connected between the flanges. The activated carbon filter plate has several filter holes inside. A first internal threaded sleeve is provided on one side of the activated carbon filter plate. The first internal threaded sleeve is threaded with the drive shaft. Rubber cleaning rods are connected to both sides of the outer wall of the first internal threaded sleeve. The rubber cleaning rods are in contact with the activated carbon filter plate.

[0015] Preferably, the purification instrument body further includes:

[0016] The protective mechanism is installed on the outer wall of the main body of the purifier and is used to synchronously protect the opening at the end of the fixed tube when the main body of the purifier moves.

[0017] The protective mechanism includes a protective plate. A first servo motor is fixedly connected to the outer wall of the purifier body. A swing rod that is rotatably connected to the output end of the first servo motor is fixedly connected to the outer wall of the purifier body. A connecting rod is slidably connected to the outer wall of the swing rod. A lifting rod that is slidably connected to the side wall of the purifier body is fixedly connected to the outer wall of the connecting rod.

[0018] Preferably, the bottom of the lifting rod is movably connected to a movable wheel, the outer wall of the swing rod is slidably connected to a sliding rod that is slidably connected to the outer wall of the purifier body, and the outer wall of the sliding rod is fixedly connected to a protective plate located at the end of the fixed tube.

[0019] Preferably, the outer wall of the swing rod and the connection part of the connecting rod are provided with a first limiting groove, the side wall of the purifier body and the connection part of the lifting rod are provided with a fixing groove, the outer wall of the swing rod and the connection part of the sliding rod are provided with a second limiting groove, the outer wall of the purifier body and the connection part of the sliding rod are provided with a sliding groove, the lifting rod forms a lifting structure through the connecting rod and the first limiting groove and the fixing groove, the lifting rod is provided in two sets, the positions of the two sets of lifting rods are symmetrical about the central axis of the purifier body, the moving wheels are provided in two sets, the positions of the two sets of moving wheels are symmetrical about the central axis of the lifting rod.

[0020] Preferably, the sliding rod forms a lifting structure with the swing rod and the second limiting groove and the sliding groove. The positions of the first limiting groove and the second limiting groove are equidistant from the rotation center of the swing rod. The outer wall contour of the protective plate is larger than the end opening contour of the fixed tube.

[0021] Preferably, it further includes:

[0022] The fixing mechanism is installed on the outer wall of the fixing pipe and is used to assist in the fixing and installation of the fixing pipe and connecting pipe;

[0023] The fixing mechanism includes a clamping block. A support block that fits against the outer wall of the connecting pipe is fixedly connected to the outer wall of the lifting rod. A connecting frame is fixedly connected to the outer wall of the lifting rod. A second servo motor is fixedly connected to the outer wall of the connecting frame. A rotating rod that is rotatably connected to the outer wall of the connecting frame is fixedly connected to the output end of the second servo motor. A clamping block that is slidably connected to the outer wall of the connecting frame is slidably connected to the outer wall of the rotating rod. A connecting groove is provided at the connection part between the outer wall of the rotating rod and the clamping block. The outer wall contours of the support block and the clamping block are semi-circular. The clamping block forms a lifting structure with the connecting frame through the rotating rod and the connecting groove.

[0024] Preferably, it further includes:

[0025] The shock absorption mechanism is located on the outer wall of the main body of the purifier;

[0026] The shock absorption mechanism includes a damper body and an adjusting block. The outer wall of the purifier body is detachably connected to a fixing block. The outer wall of the fixing block has a U-shaped outline. There are four sets of fixing blocks, and the positions of the four sets of fixing blocks are distributed around the purifier body. A nut is provided on the top of the fixing block. The inner wall of the nut is threaded with a bolt that abuts against the outer wall of the purifier body. A connecting block is fixedly connected to the bottom of the fixing block.

[0027] Preferably, a damper body is fixedly connected to the bottom of the connecting block, an adjusting block is fixedly connected to the bottom of the damper body, a support seat that contacts the ground is slidably connected to the outer wall of the adjusting block, a spring that is fixedly connected to the inner wall of the support seat is fixedly connected to the bottom of the adjusting block, and an anti-slip pad is provided at the bottom of the support seat.

[0028] Preferably, a bevel gear set is rotatably connected to the inner wall of the support base, and a knob is fixedly connected to the outer wall of the bevel gear set via a rotating shaft. A threaded rod that is rotatably connected to the inner wall of the support base is fixedly connected to the outer wall of the bevel gear set. An adjustment groove is provided at the connection between the inner wall of the support base and the adjustment block. The adjustment block forms a lifting structure with the bevel gear set, the threaded rod, and the adjustment groove. The threaded rod and the adjustment block are connected by a thread.

[0029] Compared with the prior art, the beneficial effects of this invention are as follows:

[0030] The crushing blades inside the crushing tube of this invention work in conjunction with the inner serrated ring to achieve dual crushing of materials, improve crushing uniformity, and lay the foundation for subsequent extraction and purification; the activated carbon filter plate filters simultaneously, and the rubber cleaning rod automatically cleans to avoid clogging and reduce maintenance costs.

[0031] The protective mechanism of this invention is linked to the moving wheels. When the equipment moves, the protective plate automatically closes the opening of the protective fixing tube, and when it is fixed, it automatically opens, without the need for manual operation, thus avoiding pollution and damage.

[0032] The fixing mechanism of this invention uses a clamping block and a support block to perform secondary fixing of the fixing pipe and the connecting pipe, making up for the deficiencies of the flange connection and preventing loosening and falling off.

[0033] The damper body and spring in this invention work together to reduce vibration and buffer the vibration of the equipment during operation; the damping intensity can be adjusted according to the working conditions by adjusting the height of the adjustment block by knob, adapting to different usage scenarios and ensuring extraction accuracy.

[0034] This invention integrates functions of crushing, filtering, extraction, purification, protection, fixation, and shock absorption, simplifying the operation process and improving overall work efficiency. It is suitable for the large-scale extraction and purification of *Ulva lactuca* nucleic acid and *Andrias davidianus* peptide magnetic beads. Attached Figure Description

[0035] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0036] Figure 2 This is a schematic diagram of the overall side view structure of the present invention;

[0037] Figure 3 This is a schematic diagram of the overall rear view structure of the present invention;

[0038] Figure 4This is a schematic diagram of the connection structure between the connecting rod and the lifting rod of the present invention;

[0039] Figure 5 This is a schematic diagram of the connection structure between the lifting rod and the fixing groove of the present invention;

[0040] Figure 6 This is a schematic diagram of the positional distribution of the support block and clamping block of the present invention;

[0041] Figure 7 This is a schematic diagram of the connection structure between the fixing block and the main body of the purification instrument according to the present invention;

[0042] Figure 8 This is a schematic diagram of the connection structure between the bevel gear set and the threaded rod of the present invention;

[0043] Figure 9 This is a schematic diagram of the connection structure between the adjusting block and the adjusting groove of the present invention;

[0044] Figure 10 For the present invention Figure 5 Enlarged structural diagram at point A in the diagram;

[0045] Figure 11 This is a schematic diagram of the electric pump structure of the present invention;

[0046] Figure 12 For the present invention Figure 5 Enlarged structural diagram at point A in the diagram;

[0047] Figure 13 This is a schematic diagram of the internal structure of the crushing tube of the present invention.

[0048] The labels in the attached diagram are:

[0049] 1. Purification instrument main body;

[0050] 2. Electric pump; 201. Turbine cover; 202. Outlet; 203. Crushing pipe; 204. Drive shaft; 205. Inner serrated ring; 206. Flange; 207. Activated carbon filter plate; 208. Filter hole; 209. Crushing blade; 210. First internal threaded sleeve; 211. Rubber cleaning rod; 212. Second internal threaded sleeve;

[0051] 3. Fixing pipe; 4. Mounting block; 5. Storage cylinder; 6. Power control cabinet; 7. Connecting pipe;

[0052] 8. Protective mechanism; 801. First servo motor; 802. Swing rod; 803. Connecting rod; 804. First limiting groove; 805. Lifting rod; 806. Fixing groove; 807. Moving wheel; 808. Sliding rod; 809. Sliding groove; 810. Protective plate; 811. Second limiting groove;

[0053] 9. Fixing mechanism; 901. Support block; 902. Connecting frame; 903. Second servo motor; 904. Rotating rod; 905. Clamping block; 906. Connecting groove;

[0054] 10. Shock absorption mechanism; 1001. Fixing block; 1002. Nut; 1003. Bolt; 1004. Connecting block; 1005. Damper body; 1006. Adjusting block; 1007. Support base; 1008. Spring; 1009. Anti-slip pad; 1010. Bevel gear set; 1011. Knob; 1012. Threaded rod; 1013. Adjusting groove. Detailed Implementation

[0055] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with the accompanying drawings and preferred embodiments, is provided below.

[0056] Example 1:

[0057] Please see Figures 1 to 10 This embodiment provides an integrated extraction and purification instrument for *Ulva lactuca* nucleic acid and *Andrias davidianus* peptide magnetic beads, including a purification instrument body 1, an electric pump 2 installed on the outer wall of the purification instrument body 1, a fixed pipe 3 connected to the outer wall of the electric pump 2 via a delivery pipe, an installation block 4 fixedly connected to the outer wall of the purification instrument body 1, a storage cylinder 5 installed on the outer wall of the purification instrument body 1, a power control cabinet 6 installed on the outer wall of the purification instrument body 1, and a connecting pipe 7 detachably connected to the end of the fixed pipe 3 via a flange.

[0058] Example 2:

[0059] Based on Example 1, an electric pump 2 is further disclosed.

[0060] like Figure 11-13 As shown, the electric pump 2 includes:

[0061] A turbine cover 201 is connected to one end of the electric pump 2. One end of the turbine cover 201 is connected to a water inlet. The outer wall of the turbine cover 201 is connected to a water outlet 202. The output end of the electric pump 2 is connected to a drive shaft 204. A turbine is installed inside the turbine cover 201. The drive shaft 204 is fixedly connected to the turbine. The electric pump 2 is fixed to the outer wall of the purifier body 1. The turbine cover 201 is connected to the output end of the electric pump 2. The drive shaft 204 passes through the turbine cover 201 and is fixed to the turbine. The crushing pipe 203 is connected to the water inlet of the turbine cover 201 through a flange 206.

[0062] A flange 206 is connected to one end of the inlet, and a crushing pipe 203 is fixed to one end of the flange 206. Several second internal threaded sleeves 212 are provided inside the crushing pipe 203. The second internal threaded sleeves 212 are threadedly engaged with the drive shaft 204. Several crushing blades 209 are fixed to the outer wall of the second internal threaded sleeves 212. An inner serrated ring 205 is threadedly connected to the inner wall of the crushing pipe 203. Several serrated blades are fixed to the inner wall of the inner serrated ring 205. The second internal threaded sleeves 212 are installed inside the crushing pipe 203 so that they are threadedly engaged with the drive shaft 204. The crushing blades 209 are welded to the outer wall of the second internal threaded sleeves 212. The inner serrated ring 205 is threadedly connected to the inner wall of the crushing pipe 203 to ensure that the serrated blades face the crushing blades 209.

[0063] An activated carbon filter plate 207 is connected between flanges 206. The activated carbon filter plate 207 has several filter holes 208 inside. A first internal threaded sleeve 210 is provided on one side of the activated carbon filter plate 207. The first internal threaded sleeve 210 is threadedly engaged with the drive shaft 204. Rubber cleaning rods 211 are connected to both sides of the outer wall of the first internal threaded sleeve 210. The rubber cleaning rods 211 are in contact with the activated carbon filter plate 207. The activated carbon filter plate 207 is installed between two sets of flanges 206. The first internal threaded sleeve 210 is sleeved on the drive shaft 204 and fits against the activated carbon filter plate 207. The rubber cleaning rods 211 are fixed to the outer wall of the first internal threaded sleeve 210 to ensure contact with the activated carbon filter plate 208.

[0064] Example 3:

[0065] Based on Embodiment 1, the protective mechanism 8 and the fixing mechanism 9 are further disclosed.

[0066] like Figure 4-6 As shown, the main body 1 of the purification instrument also includes:

[0067] The protective mechanism 8 is installed on the outer wall of the main body 1 of the purifier and is used to synchronously protect the opening at the end of the fixed tube 3 when the main body 1 of the purifier moves.

[0068] The protective mechanism 8 includes a protective plate 810. A first servo motor 801 is fixedly connected to the outer wall of the purifier body 1. A swing rod 802, which is rotatably connected to the output end of the first servo motor 801, is fixedly connected to the output end of the first servo motor 801. A connecting rod 803 is slidably connected to the outer wall of the swing rod 802. A lifting rod 805, which is slidably connected to the outer wall of the purifier body 1, is fixedly connected to the outer wall of the connecting rod 803. A moving wheel 807 is movably connected to the bottom of the lifting rod 805. A sliding rod, which is slidably connected to the outer wall of the purifier body 1, is slidably connected to the outer wall of the swing rod 802. 808, a protective plate 810 is fixedly connected to the outer wall of the sliding rod 808 at the end of the fixed tube 3. A first limiting groove 804 is provided at the connection between the outer wall of the swing rod 802 and the connecting rod 803. A fixing groove 806 is provided at the connection between the side wall of the purifier body 1 and the lifting rod 805. A second limiting groove 811 is provided at the connection between the outer wall of the swing rod 802 and the sliding rod 808. A sliding groove 809 is provided at the connection between the outer wall of the purifier body 1 and the sliding rod 808. The lifting rod 805 is connected to the connecting rod 803 and the first limiting groove 804. A lifting structure is formed between the fixed groove 806 and the lifting rod 805. Two sets of lifting rods 805 are provided, their positions symmetrical about the central axis of the purifier body 1. Two sets of moving wheels 807 are also provided, their positions symmetrical about the central axis of the lifting rods 805. The sliding rod 808 forms a lifting structure with the sliding groove 809 via the swing rod 802 and the second limiting groove 811. The first limiting groove 804 and the second limiting groove 811 are equidistant from the rotation center of the swing rod 802. The outer edge of the protective plate 810... The wall profile is larger than the opening profile at the end of the fixed tube 3. The first servo motor 801 is fixed to the outer wall of the purifier body 1. The swing rod 802 is fixed to the output end of the first servo motor 801. The connecting rod 803 is slidably connected to the swing rod 802 through the first limiting groove 804. The lifting rod 805 is fixed to the connecting rod 803 and embedded in the fixing groove 806. The moving wheel 807 is installed at the bottom of the lifting rod 805. The sliding rod 808 is slidably connected to the swing rod 802 through the second limiting groove 811 and embedded in the sliding groove 809. The protective plate 810 is fixed to the end of the sliding rod 808.

[0069] Example 4:

[0070] Based on Embodiment 1, a fixing mechanism 9 is further disclosed.

[0071] The fixing mechanism 9 is set on the outer wall of the fixing pipe 3 and is used to assist in the fixing and installation of the fixing pipe 3 and the connecting pipe 7.

[0072] like Figure 7As shown, the fixing mechanism 9 includes a clamping block 905. A support block 901, which fits against the outer wall of the connecting pipe 7, is fixedly connected to the outer wall of the lifting rod 805. A connecting frame 902 is fixedly connected to the outer wall of the lifting rod 805. A second servo motor 903 is fixedly connected to the outer wall of the connecting frame 902. A rotating rod 904, which is rotatably connected to the outer wall of the connecting frame 902, is fixedly connected to the output end of the second servo motor 903. A clamping block 905, which is slidably connected to the outer wall of the connecting frame 902, is slidably connected to the outer wall of the rotating rod 904. The outer wall of the rotating rod 904 and the clamping block... The connecting part of 905 has a connecting groove 906. The outer wall contours of the support block 901 and the clamping block 905 are semi-circular. The clamping block 905 forms a lifting structure with the connecting frame 902 through the rotating rod 904 and the connecting groove 906. The support block 901 is welded to the outer wall of the lifting rod 805. The connecting frame 902 is fixed on the lifting rod 805. The second servo motor 903 is fixed to the outer wall of the connecting frame 902. The rotating rod 904 is fixed to the output end of the second servo motor 903. The clamping block 905 is slidably connected to the rotating rod 904 through the connecting groove 906.

[0073] Example 5:

[0074] Based on Embodiment 1, a shock absorption mechanism 10 is further disclosed.

[0075] like Figures 8-9 As shown, the shock absorption mechanism 10 is installed on the outer wall of the main body 1 of the purifier;

[0076] The shock absorption mechanism 10 includes a damper body 1005 and an adjusting block 1006. A fixing block 1001 is detachably connected to the outer wall of the purifier body 1. The outer wall of the fixing block 1001 has a U-shaped outline. Four sets of fixing blocks 1001 are arranged, with each set distributed around the perimeter of the purifier body 1. A nut 1002 is provided at the top of the fixing block 1001, and a bolt 1003 is threaded onto the inner wall of the nut 1002 to abut against the outer wall of the purifier body 1. A connecting block 1004 is fixedly connected to the bottom of the fixing block 1001, and the damper body 1006 is fixedly connected to the bottom of the connecting block 1004. 5. An adjusting block 1006 is fixedly connected to the bottom of the damper body 1005. A support seat 1007 that contacts the ground is slidably connected to the outer wall of the adjusting block 1006. A spring 1008 that is fixedly connected to the inner wall of the support seat 1007 is fixedly connected to the bottom of the adjusting block 1006. An anti-slip pad 1009 is provided at the bottom of the support seat 1007. A bevel gear set 1010 is rotatably connected to the inner wall of the support seat 1007. A knob 1011 is fixedly connected to the outer wall of the bevel gear set 1010 via a rotating shaft. A threaded rod that is rotatably connected to the inner wall of the support seat 1007 is fixedly connected to the outer wall of the bevel gear set 1010. 1012, an adjustment groove 1013 is provided at the connection between the inner wall of the support base 1007 and the adjustment block 1006. The adjustment block 1006 forms a lifting structure with the adjustment groove 1013 through a bevel gear set 1010 and a threaded rod 1012. The threaded rod 1012 is threadedly connected to the adjustment block 1006. The inner wall contour of the support base 1007 is larger than the outer wall contour of the connecting block 1004. Two sets of adjustment grooves 1013 are provided, and the positions of the two sets of adjustment grooves 1013 are symmetrical about the central axis of the adjustment block 1006. Four sets of fixing blocks 1001 are fixed by bolts 1003 and nuts 1002. Around the main body 1 of the purifier, a connecting block 1004 is fixed to the bottom of a fixed block 1001. A damper body 1005 connects the connecting block 1004 and the adjusting block 1006. The adjusting block 1006 is embedded in the adjusting groove 1013 of the support base 1007. A spring 1008 connects the adjusting block 1006 and the inner wall of the support base 1007. A bevel gear set 1010 is installed on the inner side of the support base 1007. A threaded rod 1012 is fixed to the bevel gear set 1010 and threadedly engaged with the adjusting block 1006. A knob 1011 is connected to the bevel gear set 1010 through a rotating shaft. An anti-slip pad 1009 is pasted on the bottom of the support base 1007.

[0077] This embodiment provides an integrated extraction and purification instrument for *Ulva lactuca* nucleic acid and *Andrias davidianus* peptides using magnetic beads. The assembly process is as follows:

[0078] The electric pump 2 is fixed to the outer wall of the purifier body 1. The turbine cover 201 is connected to the output end of the electric pump 2. The drive shaft 204 passes through the turbine cover 201 and is fixed to the turbine. The crushing pipe 203 is connected to the water inlet of the turbine cover 201 through the flange 206.

[0079] A second internal threaded sleeve 212 is installed inside the crushing tube 203 so that it is threadedly engaged with the drive shaft 204. The crushing blade 209 is welded to the outer wall of the second internal threaded sleeve 212. The inner serrated ring 205 is threadedly connected to the inner wall of the crushing tube 203 to ensure that the serrated blade faces the crushing blade 209.

[0080] The activated carbon filter plate 207 is installed between two sets of flanges 206. The first internal threaded sleeve 210 is sleeved on the drive shaft 204 and fits against the activated carbon filter plate 207. The rubber cleaning rod 211 is fixed to the outer wall of the first internal threaded sleeve 210 to ensure contact with the activated carbon filter plate 208.

[0081] The protective mechanism 8 is assembled as follows: the first servo motor 801 is fixed to the outer wall of the purifier body 1, the swing rod 802 is fixed to the output end of the first servo motor 801, the connecting rod 803 is slidably connected to the swing rod 802 through the first limiting groove 804, the lifting rod 805 is fixed to the connecting rod 803 and embedded in the fixing groove 806, and the moving wheel 807 is installed at the bottom of the lifting rod 805; the sliding rod 808 is slidably connected to the swing rod 802 through the second limiting groove 811 and embedded in the sliding groove 809, and the protective plate 810 is fixed to the end of the sliding rod 808.

[0082] Assembly of fixing mechanism 9: support block 901 is welded to the outer wall of lifting rod 805, connecting frame 902 is fixed on lifting rod 805, second servo motor 903 is fixed to the outer wall of connecting frame 902, rotating rod 904 is fixed to the output end of second servo motor 903, and clamping block 905 is slidably connected to rotating rod 904 through connecting groove 906.

[0083] The shock absorption mechanism 10 is assembled as follows: four sets of fixing blocks 1001 are fixed around the body of the purifier 1 by bolts 1003 and nuts 1002; connecting blocks 1004 are fixed to the bottom of fixing blocks 1001; damper body 1005 connects connecting blocks 1004 and adjusting blocks 1006; adjusting blocks 1006 are embedded in the adjusting grooves 1013 of support base 1007; spring 1008 connects adjusting blocks 1006 and the inner wall of support base 1007; bevel gear set 1010 is installed on the inner side of support base 1007; threaded rod 1012 is fixed to bevel gear set 1010 and threadedly engaged with adjusting block 1006; knob 1011 is connected to bevel gear set 1010 through a rotating shaft; anti-slip pad 1009 is pasted on the bottom of support base 1007.

[0084] The storage cylinder 5 and the power control cabinet 6 are fixed on the outer wall of the main body 1 of the purifier. The fixed pipe 3 is connected to the outlet 202 of the electric pump 2. The connecting pipe 7 is connected to the end of the fixed pipe 3 through a flange.

[0085] The equipment operation process is as follows:

[0086] Equipment fixing and protection switching: The first servo motor 801 is started via the power control cabinet 6, driving the swing arm 802 to rotate clockwise. This causes the connecting rod 803 to move downwards via the first limiting groove 804, and the lifting rod 805 to move downwards along the fixing groove 806. The moving wheel 807 is then lifted off the ground, and the equipment is fixed by the support base 1007. Simultaneously, the swing arm 802 drives the sliding rod 808 to move upwards along the sliding groove 809 via the second limiting groove 811, opening the protective plate 810 and exposing the opening at the end of the fixing tube 3. Conversely, when the swing arm 802 rotates counterclockwise, the moving wheel 807 touches the ground, and the protective plate 810 closes for protection.

[0087] Connecting pipe fixing: Start the second servo motor 903 to drive the rotating rod 904 to rotate, which drives the clamping block 905 to move down through the connecting groove 906, and cooperates with the support block 901 to clamp the connecting pipe 7, completing the secondary fixing; when disassembling, the second servo motor 903 rotates in the opposite direction, and the clamping block 905 moves up to release.

[0088] Vibration damping adjustment: According to the vibration of the equipment, rotate the knob 1011, which drives the threaded rod 1012 to rotate through the bevel gear set 1010. The adjusting block 1006 rises and falls along the adjusting groove 1013, changing the compression of the spring 1008 to adapt to different vibration damping requirements. The damper body 1005 works with the spring 1008 to buffer vibration, and the anti-slip pad 1009 enhances the stability of the equipment.

[0089] Extraction and purification operation: The material to be processed is injected into the crushing pipe 203, the electric pump 2 is started, and the drive shaft 204 is driven to rotate, which drives the turbine to rotate and generate negative pressure. At the same time, the second internal threaded sleeve 212 and the crushing blade 209 are rotated, which cooperate with the saw teeth of the inner sawtooth ring 205 to crush the material. The crushed material is filtered through the filter holes 208 of the activated carbon filter plate 207 to remove impurities. The drive shaft 204 simultaneously drives the first internal threaded sleeve 210 and the rubber cleaning rod 211 to rotate, cleaning the surface of the activated carbon filter plate 207 to remove impurities and prevent clogging. The filtered material enters the storage cylinder 5 through the fixed pipe 3 and the connecting pipe 7 to complete the extraction and purification operation.

[0090] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. An integrated extraction and purification instrument for *Ulva lactuca* nucleic acid and *Gymnocalycium mihanovichii* peptide magnetic beads, comprising a purification instrument body (1), an electric pump (2) connected to the outer wall of the purification instrument body (1), and a fixed tube (3) connected to the electric pump (2), characterized in that: The outer wall of the purification instrument body (1) is fixedly connected to the mounting block (4), the outer wall of the purification instrument body (1) is provided with a storage cylinder (5), the outer wall of the purification instrument body (1) is provided with a power control cabinet (6), and the end of the fixed tube (3) is detachably connected to a connecting tube (7) through a flange. The electric pump (2) includes: A turbine cover (201) is connected to one end of an electric pump (2). One end of the turbine cover (201) is connected to an inlet. An outlet (202) is connected to the outer wall of the turbine cover (201). A drive shaft (204) is connected to the output end of the electric pump (2). A turbine is installed inside the turbine cover (201). The drive shaft (204) is fixedly connected to the turbine. A flange (206) is connected to one end of the inlet. A crushing pipe (203) is fixed to one end of the flange (206).

2. The integrated extraction and purification instrument for *Ulva lactuca* nucleic acid and *Andrias davidianus* peptides using magnetic beads according to claim 1, characterized in that: The crushing tube (203) is provided with a number of second internal threaded sleeves (212), which are threadedly engaged with the drive shaft (204). A number of crushing blades (209) are fixed on the outer wall of the second internal threaded sleeves (212). An inner serrated ring (205) is threadedly connected to the inner wall of the crushing tube (203), and a number of serrated blades are fixed on the inner wall of the inner serrated ring (205). An activated carbon filter plate (207) is connected between the flanges (206). The activated carbon filter plate (207) has several filter holes (208) inside. A first internal threaded sleeve (210) is provided on one side of the activated carbon filter plate (207). The first internal threaded sleeve (210) is threadedly engaged with the drive shaft (204). Rubber cleaning rods (211) are connected to both sides of the outer wall of the first internal threaded sleeve (210). The rubber cleaning rods (211) are in contact with the activated carbon filter plate (207).

3. The integrated extraction and purification instrument for *Ulva lactuca* nucleic acid and *Andrias davidianus* peptide magnetic beads according to claim 1, characterized in that, The main body of the purification instrument (1) also includes: The protective mechanism (8) is set on the outer wall of the main body (1) of the purifier and is used to protect the opening at the end of the fixed tube (3) synchronously when the main body (1) of the purifier moves. The protective mechanism (8) includes a protective plate (810). A first servo motor (801) is fixedly connected to the outer wall of the purifier body (1). A swing rod (802) that is rotatably connected to the output end of the first servo motor (801) is fixedly connected to the outer wall of the purifier body (1). A connecting rod (803) is slidably connected to the outer wall of the swing rod (802). A lifting rod (805) that is slidably connected to the side wall of the purifier body (1) is fixedly connected to the outer wall of the connecting rod (803).

4. The integrated extraction and purification instrument for *Gnaphalium affine* nucleic acid and *Andrias davidianus* peptide magnetic beads according to claim 3, characterized in that: The bottom of the lifting rod (805) is movably connected to a moving wheel (807), the outer wall of the swing rod (802) is slidably connected to a sliding rod (808) which is slidably connected to the outer wall of the purification instrument body (1), and the outer wall of the sliding rod (808) is fixedly connected to a protective plate (810) located at the end of the fixed tube (3).

5. The integrated extraction and purification instrument for *Gnaphalium affine* nucleic acid and *Gnaphalium affine* peptide magnetic beads according to claim 4, characterized in that: The outer wall of the swing rod (802) and the connection part of the connecting rod (803) are provided with a first limiting groove (804). The side wall of the purification instrument body (1) and the connection part of the lifting rod (805) are provided with a fixing groove (806). The outer wall of the swing rod (802) and the connection part of the sliding rod (808) are provided with a second limiting groove (811). The outer wall of the purification instrument body (1) and the connection part of the sliding rod (808) are provided with a sliding groove (809). The lifting rod (805) forms a lifting structure through the connecting rod (803), the first limiting groove (804) and the fixing groove (806). There are two sets of lifting rods (805). The positions of the two sets of lifting rods (805) are symmetrical about the central axis of the purification instrument body (1). There are two sets of moving wheels (807). The positions of the two sets of moving wheels (807) are symmetrical about the central axis of the lifting rods (805).

6. The integrated extraction and purification instrument for *Gnaphalium affine* nucleic acid and *Andrias davidianus* peptide magnetic beads according to claim 5, characterized in that: The sliding rod (808) forms a lifting structure with the swing rod (802) and the second limiting groove (811) and the sliding groove (809). The positions of the first limiting groove (804) and the second limiting groove (811) are equidistant from the rotation center of the swing rod (802). The outer wall contour of the protective plate (810) is larger than the end opening contour of the fixed tube (3).

7. The integrated extraction and purification instrument for *Ulva lactuca* nucleic acid and *Andrias davidianus* peptides using magnetic beads according to claim 1, characterized in that, Also includes: The fixing mechanism (9) is set on the outer wall of the fixing pipe (3) and is used to fix the pipe (3) and the connecting pipe (7) for auxiliary fixing installation; The fixing mechanism (9) includes a clamping block (905). The outer wall of the lifting rod (805) is fixedly connected to a support block (901) that fits against the outer wall of the connecting pipe (7). The outer wall of the lifting rod (805) is fixedly connected to a connecting frame (902). The outer wall of the connecting frame (902) is fixedly connected to a second servo motor (903). The output end of the second servo motor (903) is fixedly connected to a rotating rod (904) that is rotatably connected to the outer wall of the connecting frame (902). The outer wall of the rotating rod (904) is slidably connected to a clamping block (905) that is slidably connected to the outer wall of the connecting frame (902). A connecting groove (906) is provided at the connection between the outer wall of the rotating rod (904) and the clamping block (905). The outer wall contours of the support block (901) and the clamping block (905) are semi-circular. The clamping block (905) forms a lifting structure with the connecting frame (902) through the rotating rod (904) and the connecting groove (906).

8. The integrated extraction and purification instrument for *Gnaphalium affine* nucleic acid and *Andrias davidianus* peptide magnetic beads according to claim 1, characterized in that, Also includes: The shock absorption mechanism (10) is installed on the outer wall of the main body (1) of the purifier; The shock absorption mechanism (10) includes a damper body (1005) and an adjusting block (1006). The outer wall of the purification instrument body (1) is detachably connected to a fixing block (1001). The outer wall of the fixing block (1001) is U-shaped. There are four sets of fixing blocks (1001). The positions of the four sets of fixing blocks (1001) are distributed around the purification instrument body (1). The top of the fixing block (1001) is provided with a nut (1002). The inner wall of the nut (1002) is threaded with a bolt (1003) that abuts against the outer wall of the purification instrument body (1). The bottom of the fixing block (1001) is fixedly connected to a connecting block (1004).

9. The integrated extraction and purification instrument for *Gnaphalium affine* nucleic acid and *Gnaphalium affine* peptide magnetic beads according to claim 8, characterized in that: The bottom of the connecting block (1004) is fixedly connected to a damper body (1005), the bottom of the damper body (1005) is fixedly connected to an adjusting block (1006), the outer wall of the adjusting block (1006) is slidably connected to a support seat (1007) that contacts the ground, the bottom of the adjusting block (1006) is fixedly connected to a spring (1008) that is fixedly connected to the inner wall of the support seat (1007), and the bottom of the support seat (1007) is provided with an anti-slip pad (1009).

10. The integrated extraction and purification instrument for *Gnaphalium affine* nucleic acid and *Andrias davidianus* peptide magnetic beads according to claim 9, characterized in that: A bevel gear set (1010) is rotatably connected to the inner wall of the support base (1007). A knob (1011) is fixedly connected to the outer wall of the bevel gear set (1010) via a rotating shaft. A threaded rod (1012) is fixedly connected to the outer wall of the bevel gear set (1010) and rotatably connected to the inner wall of the support base (1007). An adjustment groove (1013) is provided at the connection between the inner wall of the support base (1007) and the adjustment block (1006). The adjustment block (1006) forms a lifting structure with the bevel gear set (1010), the threaded rod (1012), and the adjustment groove (1013). The threaded rod (1012) and the adjustment block (1006) are connected by a thread.

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