Determination device and determination method for paraffin removal rate of microorganisms and bacterial liquid

Abstract

The device comprises a conical flask and a glass straight-through tube, the lower end of the glass straight-through tube extends into the conical flask, and the upper end of the glass straight-through tube is connected to a conical flask opening through a test tube buckle and a latex plug. The invention also discloses a method for measuring the paraffin removal rate of microorganisms and bacterial liquid, which comprises the following steps: introducing the glass straight-through pipe into a molten wax sample or high-wax-content crude oil, slowly rotating, taking out the glass straight-through pipe after the paraffin or high-wax-content crude oil completely wraps the liquid-immersed straight-through pipe, repeating the operation for 3-5 times to simulate a wax precipitation oil pipe in a shaft, and aging and weighing the treated straight-through pipe; preparing a fermentation culture medium, introducing a straight-through pipe into the fermented culture medium, carrying out shaking table culture, lightly washing a mycoderm adhered to the straight-through pipe with water, placing, and weighing after the surface is dried; and finally, the paraffin removal rate is calculated through weighing data. By accurately calculating the paraffin removal rate, the use of the paraffin remover can be effectively evaluated and optimized, so that the paraffin precipitation phenomenon of an oil well is reduced. This helps to improve the production and production efficiency of an oil well.

Description

Technical Field

[0001] This invention belongs to the technical field of wax removal rate determination methods, and relates to a device for determining the wax removal rate of microorganisms and bacterial solutions; it also relates to a method for determining the wax removal rate of microorganisms and bacterial solutions. Background Technology

[0002] In high-wax oil wells, wax deposition is a major problem that seriously affects normal production. Regular wax removal and prevention maintenance are necessary to maintain normal production in wax-deposited wells; otherwise, wax blockage will occur. In recent years, microbial and bacterial solution wax removal and prevention technologies have been effectively promoted in many oilfields, and their application prospects in wax-deposited oil wells are broad.

[0003] The application of microbial bacterial solutions for wax removal and prevention can effectively improve the wax removal efficiency. However, there is currently no experimental method to evaluate the wax removal effect of microorganisms and bacterial solutions. In order to accurately evaluate the wax removal effect of microorganisms and bacterial solutions on wax-covered oil wells, it is necessary to carry out research on experimental methods for wax removal using microorganisms and bacterial solutions. Summary of the Invention

[0004] The purpose of this invention is to provide a device for measuring the wax removal rate of microorganisms and bacterial solutions.

[0005] The present invention also aims to provide a method for determining the wax removal rate of microorganisms and bacterial solutions, providing a new guidance for accurately evaluating the wax removal effect of microorganisms and bacterial solutions on wax-covered oil wells.

[0006] The first technical solution adopted in this invention is: a device for determining the wax removal rate of microorganisms and bacterial solutions, comprising a conical flask and a glass straight tube, wherein the lower end of the glass straight tube extends into the conical flask and the upper end is connected to the mouth of the conical flask by a test tube clip and a latex stopper, the latex stopper is sleeved on the upper end of the glass straight tube, and the latex stopper is fixed to the mouth of the conical flask by the test tube clip.

[0007] The second technical solution adopted in this invention is: a method for determining the wax removal rate of microorganisms and bacterial solutions, as detailed below: Step 1: Clean the glass straight tube with anhydrous ethanol, wipe it dry with lint-free paper, and then weigh it; Step 2: Place the wax sample or high-wax crude oil into a beaker and heat in a water bath until completely melted; Step 3: Immerse one end of the glass straight tube into the molten wax sample or high-wax crude oil, slowly rotate the glass straight tube, and remove it after the paraffin or high-wax crude oil has completely covered the immersed glass straight tube. Let it cool at room temperature. Step 4: After the paraffin or high-wax crude oil solidifies on the pipe wall, immerse it again in the heated and melted wax sample or high-wax crude oil. Repeat this process 3-5 times to evenly coat the inner and outer walls of the glass straight tube immersion end with paraffin or high-wax crude oil, thus simulating the wax-forming tubing inside the wellbore. Step 5: Let the glass straight tube processed in Step 4 air dry at room temperature for at least 3 days and weigh it; Step 6: Prepare the fermentation medium. Immerse the wax-coated glass tube treated in Step 5 into the medium and culture it on a shaker. Step 7: Calculate the wax removal rate.

[0008] The invention is further characterized in that, In step 4, the wax coating operation is completed when the weight of the paraffin or high-wax crude oil coated on the inner and outer walls of the glass straight tube is between 1.0 and 3.0 g. Step 6 is as follows: Prepare fermentation medium according to the requirements of microbial strains, seal with heat-resistant tissue culture sealing film, sterilize and inoculate, and make three parallel samples for each group; add a latex stopper to the uncontacted end of the aged glass straight tube from step 5, fix it to the mouth of the conical flask with test tube clips, ensure that the culture medium in the conical flask covers the wax-coated section of the glass straight tube, seal with heat-resistant tissue culture sealing film, and place in a shaker for culture. After the culture is completed, take out the glass straight tube, rinse the bacterial film adhering to the glass straight tube with water, place at room temperature, and weigh after the surface dries.

[0009] Step 6 describes inoculation at a ratio of 10% bacterial strain and 90% culture medium; the shaker culture conditions are: culture at 37℃ for 7-60 days.

[0010] The product is placed at room temperature for no less than 3 days as described in step 6.

[0011] Step 7 is as follows: Let M be the weight of the empty glass tube. k The weight of the glass straight tube after wax coating is recorded as M1, and the weight of the glass straight tube after microbial treatment is recorded as M2. Wax removal rate = (M2 - M...) k ) / (M1-M k ) × 100%.

[0012] The beneficial effects of this invention are: This invention provides a method for determining the wax removal rate of microorganisms and their solutions, clarifying the wellbore conditions required before microbial wax removal and prevention operations, and providing guidance for the field implementation of microbial wax removal and prevention processes. This invention determined the wax removal rate of ten microorganisms and their solutions, including QZKC, QZYD-01, QZYUE-04, QZYUE-05, QZYUE-06, KB-NBT, KB12-TM, S110, U3, and KB, with removal rates ranging from 6.33% to 84.77%. Experimental results show that for the same bacterial solution within the same incubation period, the wax removal rates measured in three parallel samples are similar, indicating good repeatability. Accurate calculation of the wax removal rate allows for effective evaluation and optimization of wax-removing agents, thereby reducing wax buildup in oil wells. This helps maintain unobstructed oil flow in oil wells, improving production and extraction efficiency. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of the device for measuring the wax removal rate of microorganisms and bacterial solutions according to the present invention.

[0014] In the diagram, 1. latex stopper, 2. test tube clip, 3. glass straight tube. Detailed Implementation

[0015] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0016] This invention relates to a method for determining the wax removal rate of microorganisms and bacterial suspensions. The method utilizes a device for determining the wax removal rate of microorganisms and bacterial suspensions. The device includes a conical flask and a glass straight tube 3. The lower end of the glass straight tube 3 extends into the conical flask, and the upper end is connected to the mouth of the conical flask via a test tube clip 4 and a latex stopper 1. The latex stopper 1 is fitted onto the upper end of the glass straight tube and fixed to the mouth of the conical flask using a test tube clip 2. The specific operating steps are as follows: Step 1: Clean the 15mm diameter and 100mm long glass straight tube with anhydrous ethanol, wipe it dry with lint-free paper, and then weigh it. Step 2: Place the wax sample or high-wax crude oil into a glass beaker and heat in a water bath until completely melted; Step 3: Immerse one end of the glass straight tube into the molten wax sample or high-wax crude oil, slowly rotate the glass straight tube, and remove it after the paraffin or high-wax crude oil has completely covered the immersed glass straight tube. Let it cool at room temperature. Step 4: After the paraffin or high-wax crude oil solidifies on the pipe wall, immerse it again in the heated and melted wax sample or high-wax crude oil. Repeat this process 3-5 times to evenly coat the inner and outer walls of the glass straight tube at the immersion end with paraffin or high-wax crude oil, simulating the waxing of the tubing inside the wellbore. Continue this process until the weight of the paraffin or high-wax crude oil coated on the inner and outer walls of the glass straight tube is between 1.0 and 3.0 g. Step 5: Let the glass straight tube processed in Step 4 air dry at room temperature for at least 3 days and weigh it; Step 6: Prepare the fermentation medium. Immerse the wax-coated glass tube treated in Step 5 into the medium and culture it on a shaker. Prepare fermentation medium according to the requirements of microbial strains, seal with heat-resistant tissue culture sealing film, and inoculate at a ratio of 10% (inoculation is carried out at a ratio of 10% strain and 90% culture medium), and make three parallel samples for each group; add a latex stopper to the uncontacted end of the aged glass straight tube from step 5, fix it to the mouth of the conical flask with test tube clips, ensure that the culture medium in the conical flask covers the wax-coated section of the glass straight tube, seal with heat-resistant tissue culture sealing film, place in a shaker, and incubate at 37℃ for 7-60 days (the incubation time is determined according to the performance of the microorganisms and bacterial solution being measured). After the incubation is completed, take out the glass straight tube, gently rinse the bacterial film adhering to the glass straight tube with tap water, place at room temperature for no less than 3 days, and weigh after the surface is dry.

[0017] Step 7: Calculate the wax removal rate; record the weight of the empty glass straight tube as M. k The weight of the glass straight tube after wax coating is recorded as M1, and the weight of the glass straight tube after microbial treatment is recorded as M2. Wax removal rate = (M2 - M...) k ) / (M1-M k ) × 100%.

[0018] Example 2 This invention was used to determine the wax removal rate of five microorganisms and their solutions: QZKC, QZYD-01, QZYUE-04, QZYUE-05, and QZYUE-06. A 15mm diameter, 100mm long glass straight tube was cleaned with anhydrous ethanol, dried with lint-free paper, and weighed. The wax samples were all high-wax crude oil samples from the Yuejin No. 2 oilfield. The crude oil sample was placed in a glass beaker and heated in a water bath at 80℃ until completely melted. Approximately 50mm of one end of the glass straight tube was immersed in the melted wax sample or high-wax crude oil. The tube was slowly rotated until the high-wax crude oil completely coated the immersed tube. It was then removed and cooled to room temperature. After the high-wax crude oil solidified on the tube wall, it was immersed again in the heated and melted high-wax crude oil. This process was repeated three times. The treated straight tube was then air-dried at room temperature and aged for three days before being weighed.

[0019] Fermentation medium was prepared and dispensed into 250ml Erlenmeyer flasks. The flasks were sealed with heat-resistant tissue culture sealing film, sterilized, and inoculated at 10% concentration, with three replicates per group. A latex stopper was added to the other end of the aged straight tube, and the tube was secured to the Erlenmeyer flask mouth with a clamp, ensuring the liquid in the flask completely submerged the wax-coated section of the straight tube. The flask was then sealed with heat-resistant tissue culture sealing film and incubated on a shaker for 15 days. The straight tube was then removed, and the bacterial film adhering to it was gently rinsed with tap water. It was then air-dried at room temperature and aged for 3 days before being weighed. The results are shown in Table 1. Table 1. Results of wax removal rate determination for five oil-producing bacteria after 15 days of culture.

[0020] Example 3: This invention was used to determine the wax removal rate of five microorganisms and their solutions: KB-NBT, KB12-TM, S110, U3, and KB. A 15mm diameter, 100mm long glass straight tube was cleaned with anhydrous ethanol, dried with lint-free paper, and weighed. The wax samples were all high-wax crude oil samples from the Yingdong Oilfield. The crude oil sample was placed in a glass beaker and heated in a water bath at 80℃ until completely melted. Approximately 50mm of one end of the glass straight tube was immersed in the melted wax sample or high-wax crude oil. The tube was slowly rotated until the high-wax crude oil completely coated the immersed tube. It was then removed and cooled to room temperature. After the high-wax crude oil solidified on the tube wall, it was immersed again in the heated and melted high-wax crude oil. This process was repeated three times. The treated straight tube was then air-dried at room temperature and aged for three days before being weighed.

[0021] Fermentation medium was prepared and dispensed into 250ml Erlenmeyer flasks. The flasks were sealed with heat-resistant tissue culture sealing film, sterilized, and inoculated at 10% concentration, with three replicates per group. A latex stopper was added to the other end of the aged straight tube, and the tube was secured to the Erlenmeyer flask mouth with a clamp, ensuring the liquid in the flask completely covered the wax-coated section of the straight tube. The flask was then sealed with heat-resistant tissue culture sealing film and incubated on a shaker for 60 days. The straight tube was then removed, and the bacterial film adhering to it was gently rinsed with tap water. It was then air-dried at room temperature and aged for 3 days before being weighed. The results are shown in Table 2. Table 2. Results of wax removal rate determination for five oil-producing bacteria after 60 days of culture.

Claims

1. A device for determining the wax removal rate of microorganisms and bacterial solutions, characterized in that, The device includes a conical flask and a glass straight tube (3). The lower end of the glass straight tube (3) extends into the conical flask and the upper end is connected to the mouth of the conical flask by a test tube clip (2) and a latex stopper (1). The latex stopper (1) is fitted onto the upper end of the glass straight tube (3) and is fixed to the mouth of the conical flask by the test tube clip (2).

2. A method for determining the wax removal rate of microorganisms and bacterial solutions, characterized in that, Specifically as follows: Step 1: Clean the glass straight tube with anhydrous ethanol, wipe it dry with lint-free paper, and then weigh it; Step 2: Place the wax sample or high-wax crude oil into a glass beaker and heat in a water bath until completely melted; Step 3: Immerse one end of the glass straight tube into the molten wax sample or high-wax crude oil, slowly rotate the glass straight tube, and remove it after the paraffin or high-wax crude oil has completely covered the immersed glass straight tube. Let it cool at room temperature. Step 4: After the paraffin or high-wax crude oil solidifies on the pipe wall, immerse it again in the heated and melted wax sample or high-wax crude oil. Repeat this process 3-5 times to evenly coat the inner and outer walls of the glass straight tube immersion end with paraffin or high-wax crude oil, thus simulating the wax-forming tubing inside the wellbore. Step 5: Let the glass straight tube processed in Step 4 air dry and age at room temperature for no less than 3 days, then weigh it; Step 6: Prepare the fermentation medium. Immerse the wax-coated glass tube treated in Step 5 into the medium and culture it on a shaker. Step 7: Calculate the wax removal rate.

3. The method for determining the wax removal rate of microorganisms and bacterial solutions according to claim 2, characterized in that, In step 4, the wax coating operation is completed when the weight of paraffin or high-wax crude oil coated on the inner and outer walls of the glass straight tube is between 1.0 and 3.0 g.

4. The method for determining the wax removal rate of microorganisms and bacterial solutions according to claim 2, characterized in that, Step 6 is as follows: Prepare fermentation medium according to the requirements of microbial strains, seal with heat-resistant tissue culture sealing film, sterilize and inoculate, and make three parallel samples for each group; add a latex stopper to the uncontacted end of the aged glass straight tube from step 5, fix it to the mouth of the conical flask with test tube clips, ensure that the culture medium in the conical flask covers the wax-coated section of the glass straight tube, seal with heat-resistant tissue culture sealing film, and place in a shaker for culture. After the culture is completed, take out the glass straight tube, rinse the bacterial film adhering to the glass straight tube with water, place at room temperature, and weigh after the surface dries.

5. The method for determining the wax removal rate of microorganisms and bacterial solutions according to claim 4, characterized in that, Step 6 describes inoculation at a ratio of 10% bacterial strain and 90% culture medium; the shaker culture conditions are: culture at 37℃ for 7-60 days.

6. The method for determining the wax removal rate of microorganisms and bacterial solutions according to claim 4, characterized in that, The product is placed at room temperature for no less than 3 days as described in step 6.

7. The method for determining the wax removal rate of microorganisms and bacterial solutions according to claim 2, characterized in that, Step 7 is as follows: Let M be the weight of the empty glass tube. k The weight of the glass straight tube after wax coating is recorded as M1, and the weight of the glass straight tube after microbial treatment is recorded as M2. Wax removal rate = (M2 - M...) k ) / (M1-M k ) × 100%.

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