Spina date seed, lily and longan aril vinegar beverage helpful for improving sleep as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of functional food, and discloses a spina date seed, lily and longan aril vinegar beverage helpful for improving sleep, and the vinegar beverage is prepared from the following raw materials in parts by weight: 10-40 parts of Shanxi mature vinegar, 10-15 parts of spina date seed, 5-12 parts of lily and 10-15 parts of longan aril. The vinegar drink is based on Shanxi mature vinegar, the spina date seeds, the lilies and the longan aril are matched, the optimal proportion is obtained through multiple tests, the effect of improving sleep is achieved through synergistic promotion, the healthy sleep state can be maintained, and the four components have a remarkable synergistic interaction effect.

Description

A vinegar drink made with jujube seed, lily bulb, and longan pulp that helps improve sleep, its preparation method, and its application. Technical Field

[0001] This invention belongs to the field of functional food technology, and in particular to a vinegar drink made with jujube seed, lily bulb, and longan pulp that helps improve sleep, its preparation method, and its application. Background Technology

[0002] Shanxi aged vinegar is a famous Chinese vinegar, known for its five characteristics: color, aroma, mellowness, richness, and acidity. It not only has a unique flavor but also has multiple health benefits, such as lowering blood lipids, lowering blood pressure, and anti-oxidation.

[0003] Ziziphi Spinosae Semen is rich in jujube seed saponins, alkaloids, flavonoids, and other components. It combats insomnia through multiple pathways, including regulating monoamine neurotransmitters and cytokines, inhibiting immune inflammatory responses, and modulating the hypothalamic-pituitary-adrenal axis. Its functions and indications include nourishing the heart and liver, calming the mind and soothing the nerves, astringing sweat, and promoting the production of body fluids. It is used for insomnia due to deficiency and restlessness, palpitations, and excessive dreaming, possessing multiple functional activities such as sedation, improving sleep, and neuroprotection.

[0004] Lily (Lilium brownii var. viridulum Baker) contains mainly serotonin, polysaccharides, and alkaloids. Its functions and indications include nourishing Yin and moistening the lungs, calming the mind and soothing the nerves. It is used for chronic cough due to Yin deficiency, hemoptysis, restlessness and palpitations, insomnia with excessive dreaming, and mental confusion. It also possesses immunomodulatory, antioxidant, and hypoglycemic biological activities, and can shorten sleep onset time and improve sleep quality.

[0005] Longan pulp (Longan Arillus) contains volatile compounds such as terpenes, sterols, phospholipids, tannins, and polysaccharides. Its functions and indications include: tonifying the heart and spleen, nourishing blood and calming the mind. It is used for deficiency of qi and blood, palpitations, forgetfulness, and insomnia. It also has the effects of nourishing blood and calming the mind, regulating sleep, and resisting stress.

[0006] The organic acids in Shanxi aged vinegar can promote sugar metabolism, improve vascular elasticity, reduce blood viscosity, and help reduce vascular resistance. In jujube seed, jujube seed saponin A is the most important active ingredient, which can increase the expression of GABA receptor subtypes in hippocampal neurons and has a significant sedative-hypnotic effect. The main active ingredients in jujube seed include: saponins, which are mainly three types of compounds, such as jujube seed saponins A and B, etc. These components have multiple functional activities such as sedation, sleep improvement, and neuroprotection. Among them, jujube seed saponin A is the most important active ingredient in jujube seed, with a significant sedative-hypnotic effect, increasing the expression of GABA receptor subtypes in hippocampal neurons, thereby improving sleep. Alkaloids, including jujube seed alkaloids such as jujube seed alkaloid K, jujube seed alkaloid, and magnoflorine, play an important role in antidepressant effects, mainly acting on calmodulin, epidermal growth factor receptor, etc., exerting an antidepressant effect through multiple components, multiple targets, and multiple pathways. Flavonoids in jujube seeds, such as spinosin, also possess certain pharmacological activities. Jujube seeds are also rich in protein, amino acids, fatty acids, vitamins, and trace elements, which together constitute the nutritional and pharmacological basis of jujube seeds. The main active components of lily include serotonin, an important class of bioactive substances in lily with various pharmacological effects such as sedation, hypnosis, hypoxia tolerance, and anti-fatigue. Serotonin compounds can shorten sleep onset time and improve sleep quality. Lily polysaccharides are another important class of active components with biological activities such as immunomodulation, anti-aging, and antioxidant effects. The safe and non-toxic properties of polysaccharides make them of significant value in the field of health. Lily also contains various alkaloids, which contribute to its pharmacological effects. Other components, such as phenolic glycerides, flavonoids, amino acids, and phospholipids, collectively constitute the rich nutritional components and pharmacological basis of lily.

[0007] However, due to the limitations of Shanxi aged vinegar's traditional positioning, insufficient investment in scientific research, and lack of industrial collaboration, it is currently often used as a condiment in people's daily lives, and there is relatively little research on its application in functional foods.

[0008] There are few existing vinegar drinks for improving sleep. This product uses Shanxi aged vinegar, which is fermented in a solid state and is rich in amino acids and organic acids. It is more effective in maintaining stable blood pressure, maintaining blood sugar balance and improving sleep.

[0009] Furthermore, this jujube seed, lily bulb, and longan pulp vinegar drink is based on Shanxi aged vinegar and contains added food-derived Chinese medicinal herbs that help improve sleep, reducing the impact of drug side effects and making it more acceptable to consumers. Summary of the Invention

[0010] The purpose of this invention is to overcome the shortcomings of the prior art and provide a jujube seed, lily bulb, and longan pulp vinegar drink that helps improve sleep, its preparation method, and its application.

[0011] The technical solution adopted by the present invention to solve its technical problem is: a vinegar drink made of jujube seed, lily bulb and longan pulp that helps improve sleep. The vinegar drink is made from the following ingredients in parts by weight: 10-40 parts of Shanxi aged vinegar, 10-15 parts of jujube seed, 5-12 parts of lily bulb and 10-15 parts of longan pulp.

[0012] Furthermore, the jujube seed, lily bulb, and longan pulp mentioned are all medicinal and edible ingredients.

[0013] Furthermore, the vinegar drink is made from the following ingredients in parts by weight: 20 parts Shanxi aged vinegar, 15 parts jujube seeds, 10 parts lily bulbs, and 12 parts longan pulp.

[0014] The preparation method of the jujube seed, lily bulb and longan pulp vinegar drink as described above includes the following steps: (1) Shanxi aged vinegar is concentrated by vacuum concentration to obtain concentrated liquid I; (2) jujube seed, lily bulb and longan pulp are boiled and filtered to obtain filtrate II, which is then concentrated by vacuum concentration to obtain concentrated liquid III; (3) concentrated liquid I and III are combined, excipients are added, flavor is adjusted, mixed and filtered, and further sterilized and packaged to obtain jujube seed, lily bulb and longan pulp vinegar drink that helps improve sleep.

[0015] Furthermore, the vacuum concentration conditions in steps (1) and (2) are: the vacuum degree is maintained at -0.05 to -0.09 MPa, and the heating temperature is maintained at 50 to 85°C; or, the relative density of the concentrate I in step (1) is 1.16-1.22, and it is concentrated to 0.4-0.7 times the original volume of Shanxi aged vinegar.

[0016] Further, the decoction extraction conditions for jujube seed, lily bulb and longan pulp in step (2) are as follows: weigh jujube seed, lily bulb and longan pulp, use water extraction method, add water to soak at a material-to-liquid ratio of 1:15-35 kg:L, decoct at 90-100℃ for 60-90 min, filter after decoction, decoct 2-4 times, collect and combine to obtain filtrate II; or, the relative density of concentrated liquid III in step (2) is 1.10-1.18, concentrate to 0.02-0.07 times the original volume of filtrate II.

[0017] Furthermore, the types and final mass concentrations (m / V) of the excipients in step (3) are: 1.0%-2.0% stevioside, 1.0%-5.0% honey, 1.0%-2.0% sodium bicarbonate and 0.1%-0.2% stabilizer, to adjust the texture and taste of the beverage.

[0018] Furthermore, in step (3), the mixed liquid is sterilized and packaged using ultraviolet sterilization, pasteurization or high-temperature steam sterilization to ensure that the jujube seed, lily bulb and longan pulp vinegar drink can meet the national safety requirements.

[0019] The application of the above-mentioned jujube seed, lily bulb, longan pulp vinegar drink in the preparation of health food for improving sleep quality.

[0020] Furthermore, the sour jujube seed, lily bulb, and longan pulp vinegar drink has a good effect on improving sleep quality, and the daily dosage is 10-50mL / day. It is recommended to take it twice a day, morning and evening. It is suitable as a daily health drink for people who have difficulty starting sleep (or falling asleep) and have difficulty maintaining sleep.

[0021] The advantages and positive effects of this invention are as follows: 1. The vinegar drink of this invention is based on Shanxi aged vinegar, combined with jujube seed, lily bulb and longan pulp. After multiple experiments, the optimal ratio was obtained, which synergistically promotes the effect of improving sleep and helps maintain a healthy sleep state. The four ingredients have a significant synergistic effect.

[0022] 2. The vinegar drink of this invention specifies a concentration range for Shanxi aged vinegar and Chinese herbal extracts, that is, a concentration to a range of relative densities. This can reduce the volatile components in the Chinese herbal extracts, maximize the enrichment of the effective components of the raw materials, and avoid the loss of effective components.

[0023] 3. The recommended dosage and method of consumption of the vinegar drink of this invention not only avoid the irritation of acetic acid to the human gastric mucosa, making it more acceptable to the user, but also improve the utilization rate of its active ingredients, ensuring that the sleep-improving effect is fully realized.

[0024] 4. The vinegar drink of this invention is not only suitable for people with sleep disorders and poor sleep quality, but also for young people who need to improve their sleep, providing a variety of product choices for consumers.

[0025] 5. This invention's jujube seed, lily bulb, and longan pulp vinegar drink is based on Shanxi aged vinegar, combined with jujube seeds, lily bulbs, and longan pulp to synergistically improve sleep. It is produced through steps including reduced-pressure concentration of Shanxi aged vinegar, extraction and concentration of traditional Chinese medicine, flavor blending, sterilization, and packaging. It has a herbal aroma, a mellow and slightly sour taste, and is beneficial for improving sleep. This vinegar drink is a novel and effective functional food that can improve sleep quality, suitable for daily health maintenance by people with poor sleep quality, and has broad market prospects and application value. Attached Figure Description

[0026] Figure 1 is a schematic diagram of the preparation process of the jujube seed, lily bulb, and longan pulp vinegar drink of the present invention. Detailed Implementation

[0027] The present invention will be further described below with reference to the embodiments. The following embodiments are descriptive and not limiting, and should not be used to limit the scope of protection of the present invention.

[0028] The various experimental operations involved in the specific embodiments are all conventional techniques in the art. For parts not specifically annotated herein, those skilled in the art can refer to various commonly used reference books, scientific and technological literature, or related instructions and manuals prior to the filing date of this invention for implementation. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0029] A vinegar drink made with jujube seed, lily bulb, and longan pulp that helps improve sleep, is made from the following ingredients in parts by weight: 10-40 parts Shanxi aged vinegar, 10-15 parts jujube seed, 5-12 parts lily bulb, and 10-15 parts longan pulp.

[0030] Preferably, the jujube seed, lily bulb, and longan pulp are all medicinal and edible ingredients.

[0031] Preferably, the vinegar drink is made from the following ingredients in parts by weight: 20 parts Shanxi aged vinegar, 15 parts jujube seeds, 10 parts lily bulbs, and 12 parts longan pulp.

[0032] The preparation method of the jujube seed, lily bulb and longan pulp vinegar drink as described above is shown in Figure 1, including the following steps: (1) Shanxi aged vinegar is concentrated by vacuum to obtain concentrated liquid I; (2) jujube seed, lily bulb and longan pulp are boiled and filtered to obtain filtrate II, which is then concentrated by vacuum to obtain concentrated liquid III; (3) concentrated liquid I and III are combined, excipients are added, flavor is adjusted, mixed and filtered, and further sterilized and packaged to obtain jujube seed, lily bulb and longan pulp vinegar drink that helps improve sleep.

[0033] Preferably, the vacuum concentration conditions in steps (1) and (2) are: the vacuum degree is maintained at -0.05 to -0.09 MPa, and the heating temperature is maintained at 50 to 85°C; or, the relative density of the concentrate I in step (1) is 1.16-1.22, and it is concentrated to 0.4-0.7 times the original volume of Shanxi aged vinegar.

[0034] Preferably, the decoction extraction conditions for jujube seed, lily bulb and longan pulp in step (2) are as follows: weigh jujube seed, lily bulb and longan pulp, use water extraction method, add water to soak at a material-to-liquid ratio of 1:15-35 kg:L, decoct at 90-100℃ for 60-90 min, filter after decoction, decoct 2-4 times, collect and combine to obtain filtrate II; or, the relative density of the concentrate III in step (2) is 1.10-1.18, concentrate to 0.02-0.07 times the original volume of filtrate II.

[0035] Preferably, the types and final mass concentrations (m / V) of the excipients in step (3) are: 1.0%-2.0% steviol glycoside, 1.0%-5.0% honey, 1.0%-2.0% sodium bicarbonate and 0.1%-0.2% stabilizer, for use in adjusting the texture and taste of the beverage.

[0036] Preferably, in step (3), the mixed liquid is sterilized and packaged by ultraviolet sterilization, pasteurization or high temperature steam sterilization to ensure that the jujube seed, lily bulb and longan pulp vinegar drink can meet the national safety requirements.

[0037] The application of the above-mentioned jujube seed, lily bulb, longan pulp vinegar drink in the preparation of health food for improving sleep quality.

[0038] Preferably, the jujube seed, lily bulb, and longan pulp vinegar drink has a good effect on improving sleep quality, and the daily dosage is 10-50mL / day. It is recommended to take it twice a day, morning and evening. It is suitable as a daily health drink for people who have difficulty starting sleep (or falling asleep) and have difficulty maintaining sleep.

[0039] Specifically, the relevant preparation and testing are as follows: Example 1: Preparation of a vinegar drink made from jujube seed, lily bulb, and longan pulp that helps improve sleep. A vinegar drink made from jujube seed, lily bulb, and longan pulp that helps improve sleep is mainly made from the following ingredients in parts by weight: 20 parts Shanxi aged vinegar, 15 parts jujube seed, 10 parts lily bulb, and 12 parts longan pulp.

[0040] The steps for preparing jujube seed, lily bulb, longan pulp vinegar drink using the above raw materials are as follows: (1) Shanxi aged vinegar is concentrated by vacuum concentration to obtain concentrated liquid I.

[0041] (2) The jujube seed, lily bulb and longan pulp were boiled and filtered to obtain filtrate II, which was then concentrated under reduced pressure to obtain concentrated solution III.

[0042] The vacuum concentration conditions in steps (1) and (2) are: maintaining a vacuum of -0.06 MPa and a heating temperature of 80°C.

[0043] In step (1), the relative density of concentrate I is 1.17, and it is concentrated to 0.5 times the original volume of Shanxi aged vinegar.

[0044] The extraction conditions for jujube seed, lily bulb, and longan pulp in step (2) are as follows: weigh jujube seed, lily bulb, and longan pulp, use water extraction method, soak in water at a material-to-liquid ratio of 1:20 kg:L, decoct at 100℃ for 60 min, filter after decoction, decoct 3 times, and collect and combine filtrate II. The relative density of the resulting concentrated liquid III is 1.15, and it is concentrated to 0.05 times the original volume of filtrate II.

[0045] (3) Combine concentrated liquid I and III and add appropriate excipients, mix well and filter, then sterilize and package to obtain a jujube seed, lily bulb and longan pulp vinegar drink that helps improve sleep.

[0046] The amount of the excipients added in step (3) is the percentage of the total volume of the jujube seed, lily bulb, and longan pulp vinegar drink (m / V, i.e., the final mass concentration): stevioside 2.0%, honey 1.0%, sodium bicarbonate 0.8%, and stabilizer 0.2%, to adjust the texture and taste of the drink.

[0047] In step (3), the mixture is sterilized and packaged using ultraviolet sterilization (UV light irradiation for 35 minutes) to ensure that the jujube seed, lily bulb, and longan pulp vinegar drink meets the national safety requirements.

[0048] The recommended dosage for the sour jujube seed, lily bulb, and longan pulp vinegar is 15 mL per serving, twice a day, morning and evening.

[0049] Example 2: Preparation of a Jujube Seed, Lily Bulb, and Longan Vinegar Drink that Helps Improve Sleep A jujube seed, lily bulb, and longan vinegar drink that helps improve sleep is mainly made from the following ingredients in parts by weight: 5 parts Shanxi aged vinegar, 5 parts jujube seeds, 10 parts lily bulbs, and 10 parts longan pulp.

[0050] The steps for preparing jujube seed, lily bulb, longan pulp vinegar drink using the above raw materials are as follows: (1) Shanxi aged vinegar is concentrated by vacuum concentration to obtain concentrated liquid I.

[0051] (2) The jujube seed, lily bulb and longan pulp were boiled and filtered to obtain filtrate II, which was then concentrated under reduced pressure to obtain concentrated solution III.

[0052] The vacuum concentration conditions in steps (1) and (2) are: maintaining a vacuum of -0.06 MPa and a heating temperature of 80°C.

[0053] In step (1), the relative density of concentrate I is 1.17, and it is concentrated to 0.5 times the original volume of Shanxi aged vinegar.

[0054] The extraction conditions for jujube seed, lily bulb, and longan pulp in step (2) are as follows: weigh jujube seed, lily bulb, and longan pulp, use water extraction method, soak in water at a material-to-liquid ratio of 1:20 kg:L, decoct at 100℃ for 60 min, filter after decoction, decoct twice, and collect and combine filtrate II. The relative density of the resulting concentrated liquid III is 1.15, and it is concentrated to 0.05 times the original volume of filtrate II.

[0055] (3) Combine concentrated liquid I and III and add appropriate excipients, mix well and filter, then sterilize and package to obtain a jujube seed, lily bulb and longan pulp vinegar drink that helps improve sleep.

[0056] The amount of the excipients added in step (3) is the percentage of the total volume of the jujube seed, lily bulb, and longan pulp vinegar drink (m / V, i.e., the final mass concentration): stevioside 2.0%, honey 1.0%, sodium bicarbonate 0.8%, and stabilizer 0.2%, to adjust the texture and taste of the drink.

[0057] In step (3), the mixture is sterilized and packaged using ultraviolet sterilization (UV light irradiation for 35 minutes) to ensure that the jujube seed, lily bulb, and longan pulp vinegar drink meets the national safety requirements.

[0058] The recommended dosage for the sour jujube seed, lily bulb, and longan pulp vinegar is 15 mL per serving, twice a day, morning and evening.

[0059] Example 3: Preparation of a Jujube Seed, Lily Bulb and Longan Drink to Improve Sleep A jujube seed, lily bulb and longan drink to improve sleep is mainly made from the following ingredients in parts by weight: 15 parts jujube seed, 6 parts lily bulb, and 4 parts longan pulp.

[0060] The steps for preparing jujube seed, lily bulb and longan pulp drink using the above raw materials are as follows: (1) Jujube seed, lily bulb and longan pulp are boiled and filtered to obtain filtrate II, which is then concentrated under reduced pressure to obtain concentrated liquid III.

[0061] The vacuum concentration conditions in step (1) are: maintaining a vacuum of -0.06 MPa and a heating temperature of 80°C.

[0062] The extraction conditions for jujube seed, lily bulb, and longan pulp in step (1) are as follows: weigh jujube seed, lily bulb, and longan pulp, use water extraction method, soak in water at a material-to-liquid ratio of 1:20 kg:L, decoct at 100℃ for 60 min, filter after decoction, decoct twice, and collect and combine filtrate II. The relative density of the resulting concentrated liquid III is 1.15, and it is concentrated to 0.05 times the original volume of filtrate II.

[0063] (2) Add appropriate amount of excipients to concentrated liquid III, mix well and filter, then sterilize and package to obtain a jujube seed, lily and longan pulp drink that helps improve sleep.

[0064] The amount of the auxiliary materials added in step (2) is the percentage of the total volume of the jujube seed, lily bulb and longan pulp drink (m / V, i.e., the final mass concentration): stevioside 2.0%, honey 1.0%, sodium bicarbonate 0.8%, and stabilizer 0.2%, to adjust the texture and taste of the drink.

[0065] In step (2), the mixture is sterilized and packaged using ultraviolet sterilization (UV light irradiation for 35 minutes) to ensure that the jujube seed, lily bulb and longan pulp drink meets the national safety requirements.

[0066] The recommended dosage for drinking jujube seed, lily bulb, and longan pulp is 15 mL per serving, twice a day, morning and evening.

[0067] Example 4: Preparation of Jujube Seed and Lily Vinegar Drink to Improve Sleep A jujube seed and lily vinegar drink to improve sleep is mainly made from the following ingredients in parts by weight: 10 parts Shanxi aged vinegar, 6 parts jujube seeds, and 4 parts lily bulbs.

[0068] The steps for preparing jujube seed and lily vinegar drink using the above raw materials are as follows: (1) Shanxi aged vinegar is concentrated by vacuum concentration to obtain concentrated liquid I.

[0069] (2) The jujube seed and lily bulb were boiled and filtered to obtain filtrate II, which was then concentrated under reduced pressure to obtain concentrated solution III.

[0070] The vacuum concentration conditions in steps (1) and (2) are: maintaining a vacuum of -0.06 MPa and a heating temperature of 80°C.

[0071] In step (1), the relative density of concentrate I is 1.17, and it is concentrated to 0.5 times the original volume of Shanxi aged vinegar.

[0072] The extraction conditions for jujube seed and lily bulb in step (2) are as follows: Jujube seed and lily bulb are weighed according to the above claims, and water extraction is performed. Water is added at a material-to-liquid ratio of 1:20 kg:L for soaking. The mixture is then decocted at 100℃ for 60 min. After decocting, the mixture is filtered. The decoction is repeated twice, and the filtrates II are collected and combined. The relative density of the resulting concentrated liquid III is 1.15. The concentrate is then concentrated to 0.05 times the original volume of filtrate II.

[0073] (3) Combine concentrated liquid I and III and add appropriate excipients, mix well and filter, then sterilize and package to obtain a jujube seed and lily vinegar drink that helps improve sleep.

[0074] The amount of the auxiliary materials added in step (3) is the percentage of the total volume of the jujube seed and lily vinegar drink (m / V, i.e., the final mass concentration): stevioside 2.0%, honey 1.0%, sodium bicarbonate 0.8%, and stabilizer 0.2%, to adjust the texture and taste of the drink.

[0075] In step (3), the mixture is sterilized and packaged using ultraviolet sterilization (UV light irradiation for 35 minutes) to ensure that the jujube seed and lily vinegar drink meets the national safety requirements.

[0076] The recommended dosage for drinking jujube seed and lily bulb tea is 15 mL per serving, twice a day, morning and evening.

[0077] Example 5: Preparation of a Jujube Seed, Lily Bulb, and Longan Vinegar Drink that Helps Improve Sleep A jujube seed, lily bulb, and longan vinegar drink that helps improve sleep is mainly made from the following ingredients in parts by weight: 10 parts Shanxi aged vinegar, 6 parts jujube seeds, 6 parts lily bulbs, and 4 parts longan pulp.

[0078] The steps for preparing jujube seed, lily bulb, and longan pulp vinegar drink using the above raw materials are as follows: (1) Shanxi aged vinegar is the original vinegar liquid I.

[0079] (2) The jujube seed, lily bulb and longan pulp were boiled and filtered to obtain filtrate II, which was then concentrated under reduced pressure to obtain concentrated solution III.

[0080] The vacuum concentration conditions in step (2) are: maintaining a vacuum of -0.07 MPa and a heating temperature of 80°C.

[0081] The extraction conditions for jujube seed, lily bulb, and longan pulp in step (2) are as follows: weigh jujube seed, lily bulb, and longan pulp, use water extraction, soak in water at a material-to-liquid ratio of 1:20 kg:L, decoct at 100℃ for 60 min, filter after decoction, decoct twice, and collect and combine filtrates II. The relative density of the resulting concentrated liquid III is 1.12, and it is concentrated to 0.05 times the original volume of filtrate II.

[0082] (3) Combine concentrated liquid I and III and add appropriate excipients, mix well and filter, then sterilize and package to obtain a jujube seed, lily bulb and longan pulp vinegar drink that helps improve sleep.

[0083] The amount of the auxiliary materials added in step (3) is the percentage of the total volume of jujube seed, lily bulb and longan pulp (m / V, i.e. final mass concentration): stevioside 2.0%, honey 1.0%, sodium bicarbonate 0.8% and stabilizer 0.2%, to adjust the texture and taste of the beverage.

[0084] In step (3), the mixture is sterilized and packaged using ultraviolet sterilization (UV light irradiation for 35 minutes) to ensure that the jujube seed, lily bulb, and longan pulp vinegar drink meets the national safety requirements.

[0085] The recommended dosage for the sour jujube seed, lily bulb, and longan pulp vinegar is 15 mL per serving, twice a day, morning and evening.

[0086] Comparative Example 1: A jujube seed, lily bulb, and longan pulp drink that helps improve sleep. Its raw materials and preparation method are the same as in Example 1, except that: Shanxi aged vinegar is not used, and an equal volume of distilled water is used instead of Shanxi aged vinegar.

[0087] The recommended dosage for drinking jujube seed, lily bulb, and longan pulp is 15 mL per serving, twice a day, morning and evening.

[0088] Comparative Example 2: A lily and longan vinegar drink that helps improve sleep. Its raw materials and preparation method are the same as in Example 1, except that: jujube seed is not used.

[0089] The recommended dosage for the lily and longan vinegar is 15 mL per serving, twice a day, morning and evening.

[0090] Test Example: The present invention provides a compound composition that helps improve sleep. To demonstrate its effect, the compound composition provided in the example was evaluated for efficacy. The results are as follows: 270 normal adult male mice (3 batches, 90 mice per batch) were selected as experimental animals and randomly divided into blank group, model group, experimental group 1-5, and control group 1-2, with 10 mice in each group per batch. They were acclimatized for 7 days.

[0091] Except for the control group, all other groups of animals were intraperitoneally injected with a suspension of PCPA for 3 consecutive days at a dose of 350 mg / kg. The control group was intraperitoneally injected with the same volume of 0.9% physiological saline with the same mass concentration as PCPA. Mice in the control group slept well, were in good spirits, and showed no significant changes. Mice in the model group, experimental groups 1-5, and control groups 1-2 showed a certain degree of excitability, with loss of circadian rhythm and continuous activity during the day and night, indicating that the insomnia model was successfully established.

[0092] On day 3, after intraperitoneal injection of PCPA, experimental groups 1-5 were given the prepared samples of Examples 1-5 in sequence, calculated according to the daily oral intake of 30mL of oral solution for an adult weighing 70kg. The subsequent dose for mice was 3mL / kg / d, administered twice a day in the morning and evening. Control groups 1-2 were given the prepared samples of Comparative Examples 1-2 in sequence. Normal group and model group were given an equal volume of 0.9% physiological saline by gavage for 4 consecutive weeks.

[0093] Experimental methods: After the mice completed the last administration, all batches of mice first underwent a direct sleep experiment, then the first batch of mice underwent an experiment to prolong the sleep time of sodium pentobarbital, the second batch of mice underwent a subthreshold dose hypnosis experiment of sodium pentobarbital, and the third batch of mice underwent a sodium barbital sleep latency experiment.

[0094] 1. Direct Sleep Experiment: Sixty minutes after the last administration of the test substance in the three batches of experiments, the mice were turned over, and the righting reflex was observed to determine whether the mice had fallen asleep. The observation time for the righting reflex was limited to 1 minute. If the righting reflex disappeared, it indicated that the mouse had fallen asleep, representing that the test substance had a direct sleep effect; otherwise, it had no direct sleep effect. The results are shown in Table 1.

[0095] Table 1. Effect of the examples on the rate of direct sleep in mice (n=10)

[0096] As shown in Table 1, no righting reflex disappeared within 60 minutes after gavage in any group of mice, indicating that Examples 1-5 do not have a direct sleep-inducing effect and are not similar to the mechanism of action of hypnotics.

[0097] 2. Pentobarbital sodium sleep duration prolongation experiment: 30 minutes after the last administration of the first batch of mice, sodium barbital was injected intraperitoneally at a dose of 50 mg / kg (based on mouse body weight, 100% of mice fell asleep but the sleep time was short). The sleep duration (time from the disappearance of the righting reflex to the reappearance of the righting reflex) of each group of mice was observed and recorded, and the sleep time between groups was compared. The results are shown in Table 2.

[0098] Table 2. Sleep prolongation time in mice tested with sodium pentobarbital (SPO) ±S, n=10)

[0099] Note: Compared with the control group, the experimental group showed P < 0.05. Compared with the control group, the experimental groups showed a significant difference (P < 0.01). As shown in Table 2, the sleep duration of mice in experimental groups 1-5 was increased compared with the control group. The sleep duration of mice in experimental groups 1 and 3 increased by 109.9% and 87.3% respectively, both statistically significant, with experimental group 1 showing extremely significant increases. This indicates that the experimental group 1 demonstrated a significant effect in prolonging sleep time, and the experimental results were positive. The sleep duration of mice in control groups 1-2 increased by 23.4% and 9.7% respectively, showing no significant effect.

[0100] Meanwhile, by comparing Example 1, Comparative Example 1, and Comparative Example 2, it can be seen that the raw materials Shanxi aged vinegar and jujube seeds in the method of the present invention have a synergistic effect, which can synergistically improve the relevant properties of the prepared jujube seed, lily bulb, and longan pulp vinegar drink. In particular, the raw materials Shanxi aged vinegar and jujube seeds in the method of the present invention with a mass ratio of 4:3 have a synergistic effect, which can synergistically improve the relevant properties of the prepared jujube seed, lily bulb, and longan pulp vinegar drink.

[0101] 3. Sodium barbital sleep latency experiment: 30 minutes after the last administration of the second batch of mice, sodium barbital was injected intraperitoneally at a dose of 230 mg / kg (based on mouse body weight, with 100% of mice falling asleep). The time from intraperitoneal injection to the disappearance of the righting reflex was recorded as the sleep latency, with the disappearance of the righting reflex for 60 seconds as the indicator. The difference in sleep latency between the model control group and the experimental group was compared.

[0102] Table 3. Sleep latency of mice in the sodium barbital sleep latency experiment ( ±S, n=10)

[0103] Note: The difference between the experimental group and the control group was P<0.05.

[0104] As shown in Table 3, the sleep latency of mice in experimental groups 1, 3, and 4 was significantly different from that of the control group, shortening by 30.7%, 28.4%, and 28.4%, respectively. The shortening of sleep latency was significant, and the shortening rate of sleep latency in experimental group 1 was longer than that in the other groups, indicating a positive experimental result. In contrast, the sleep latency of mice in control groups 1 and 2 was shortened by 21.6% and 5.7%, respectively, compared to the control group, with no significant difference in sleep latency shortening.

[0105] Meanwhile, by comparing Example 1, Comparative Example 1, and Comparative Example 2, it can be seen that the raw materials Shanxi aged vinegar and jujube seeds in the method of the present invention have a synergistic effect, which can synergistically improve the relevant properties of the prepared jujube seed, lily bulb, and longan pulp vinegar drink. In particular, the raw materials Shanxi aged vinegar and jujube seeds in the method of the present invention with a mass ratio of 4:3 have a synergistic effect, which can synergistically improve the relevant properties of the prepared jujube seed, lily bulb, and longan pulp vinegar drink.

[0106] 4. In the subthreshold dose hypnotic experiment of sodium pentobarbital, 30 minutes after the last administration, 40.0 mg / kg sodium pentobarbital was injected intraperitoneally at a dose of 0.01 mL / g. Under quiet conditions and a temperature of 24℃~25℃, the disappearance of the righting reflex for more than 1 minute was used as the criterion for mice to enter sleep. The sleep rate of each group of mice was observed, and the difference in the number of sleeping animals in each administration group and the blank group was compared.

[0107] Table 4. Number of mice falling asleep in subthreshold doses of sodium barbital hypnosis experiment (n=10)

[0108] Note: The difference between the experimental group and the control group was P<0.05.

[0109] As shown in Table 4, mice in experimental group 1 had a sleep count of 6, indicating a significant improvement in sleep. Mice in experimental groups 2-5 had sleep counts of 2, 5, 4, and 3 respectively, showing no improvement in sleep. Mice in control groups 1-2 had sleep counts of 4 and 2 respectively, also showing no improvement in sleep.

[0110] Meanwhile, by comparing Example 1, Comparative Example 1, and Comparative Example 2, it can be seen that the raw materials Shanxi aged vinegar and jujube seeds in the method of the present invention have a synergistic effect, which can synergistically improve the relevant properties of the prepared jujube seed, lily bulb, and longan pulp vinegar drink. In particular, the raw materials Shanxi aged vinegar and jujube seeds in the method of the present invention with a mass ratio of 4:3 have a synergistic effect, which can synergistically improve the relevant properties of the prepared jujube seed, lily bulb, and longan pulp vinegar drink.

[0111] The above experimental results show that: Example 1 group had no significant direct sleep effect; Example 1 group significantly prolonged the sleep time of mice treated with sodium pentobarbital; Example 1 group significantly shortened the sleep latency of sodium pentobarbital; and the number of mice sleeping under subthreshold doses of sodium pentobarbital in Example 1 group significantly increased. According to the "Methods for Functional Testing and Evaluation of Health Foods (2023 Edition)," if any two of the three experiments—prolonging the sleep time of sodium pentobarbital, the sleep latency of sodium pentobarbital, and the subthreshold dose hypnotic experiment—are positive, and there is no significant direct sleep effect, it can be determined that the vinegar drink of Example 1 has a sleep-improving effect. The compound composition of this invention has a significant sleep-improving effect, high safety, and can be well applied to the development and production of functional foods that help improve sleep.

[0112] In conclusion, altering the prescription and preparation method, or removing Shanxi aged vinegar or some traditional Chinese medicines, will adversely affect the sleep-improving effect. Therefore, Example 1 demonstrates the best sleep-improving effect. The jujube seed, lily bulb, and longan pulp vinegar drink of this invention has a significant sleep-improving effect, high safety, and can be well applied to the development and production of functional foods that help maintain healthy blood sugar levels.

[0113] Although embodiments of the invention have been disclosed for illustrative purposes, those skilled in the art will understand that various substitutions, variations, and modifications are possible without departing from the spirit and scope of the invention and the appended claims. Therefore, the scope of the invention is not limited to the contents disclosed in the embodiments.

Claims

1. A vinegar-based drink made with jujube seed, lily bulb, and longan pulp that helps improve sleep, characterized by: The vinegar drink is made from the following ingredients in parts by weight: 10-40 parts Shanxi aged vinegar, 10-15 parts jujube seeds, 5-12 parts lily bulbs, and 10-15 parts longan pulp.

2. The jujube seed, lily bulb, and longan pulp vinegar drink according to claim 1, characterized in that: The jujube seed, lily bulb, and longan pulp mentioned are all medicinal and edible ingredients.

3. The jujube seed, lily bulb, and longan pulp vinegar drink according to claim 1 or 2, characterized in that: The vinegar drink is made from the following ingredients in parts by weight: 20 parts Shanxi aged vinegar, 15 parts jujube seeds, 10 parts lily bulbs, and 12 parts longan pulp.

4. The preparation method of the jujube seed, lily bulb, and longan pulp vinegar drink according to any one of claims 1 to 3, characterized in that, The steps include: (1) Shanxi aged vinegar is concentrated by vacuum concentration to obtain concentrated liquid I; (2) jujube seed, lily and longan pulp are boiled and filtered to obtain filtrate II, which is then concentrated by vacuum concentration to obtain concentrated liquid III; (3) concentrated liquid I and III are combined, excipients are added, flavor is adjusted, mixed and filtered, and further sterilized and packaged to obtain jujube seed, lily and longan pulp vinegar drink that helps improve sleep.

5. The preparation method according to claim 4, characterized in that: The vacuum concentration conditions in steps (1) and (2) are: the vacuum degree is maintained at -0.05 to -0.09 MPa, and the heating temperature is maintained at 50 to 85℃; or, the relative density of the concentrate I in step (1) is 1.16-1.22, and it is concentrated to 0.4-0.7 times the original volume of Shanxi aged vinegar.

6. The preparation method according to claim 4, characterized in that: The extraction conditions for jujube seed, lily bulb and longan pulp in step (2) are as follows: weigh jujube seed, lily bulb and longan pulp, use water extraction method, add water to soak at a material-to-liquid ratio of 1:15-35 kg:L, decoct at 90-100℃ for 60-90 min, filter after decoction, decoct 2-4 times, collect and combine to obtain filtrate II; or, the relative density of concentrated liquid III in step (2) is 1.10-1.18, concentrate to 0.02-0.07 times the original volume of filtrate II.

7. The preparation method according to claim 4, characterized in that: The types and final mass concentrations (m / V) of the excipients in step (3) are: 1.0%-2.0% steviol glycoside, 1.0%-5.0% honey, 1.0%-2.0% sodium bicarbonate and 0.1%-0.2% stabilizer, which are used to adjust the texture and taste of the beverage.

8. The preparation method according to any one of claims 4 to 7, characterized in that: In step (3), the mixed liquid is sterilized and packaged using ultraviolet sterilization, pasteurization or high-temperature steam sterilization to ensure that the jujube seed, lily bulb and longan pulp vinegar drink meets the national safety requirements.

9. The use of the jujube seed, lily bulb, and longan pulp vinegar drink as described in any one of claims 1 to 3 in the preparation of health food products for improving sleep quality.

10. The application according to claim 9, characterized in that: The sour jujube seed, lily bulb, and longan pulp vinegar drink has a good effect on improving sleep quality. The daily dosage is 10-50mL / day, and it is recommended to take it twice a day, morning and evening. It is suitable as a daily health drink for people who have difficulty starting sleep (or falling asleep) and maintaining sleep.

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