Composite growth regulator for promoting seedling emergence and ear strengthening of ratooning rice in regeneration season as well as preparation method and application of composite growth regulator

Abstract

The invention provides a compound growth regulator for promoting seedling emergence and ear strengthening of ratooning rice in the regeneration season and a preparation method and application thereof.The compound growth regulator is prepared from zeatin riboside, monopotassium phosphate, a surfactant and an alkaline solution, the zeatin riboside is dissolved in the alkaline solution, a proper amount of water is added to reach the target concentration, then the monopotassium phosphate is added to be dissolved, and the surfactant is added; adding a surfactant into the solution, and uniformly mixing; the compound growth regulator is sprayed once three days before ratooning rice is harvested in the first season and one day after the ratooning rice is harvested in the first season; the dosage of the compound growth regulator is 30-50 L / mu; the riboside zeatin and the monopotassium phosphate are used as the compound growth regulator of the ratooning rice, so that the ear number per unit area, the maturing rate and the yield in the ratooning season can be remarkably increased, and the effect is remarkably superior to that of singly applying the riboside zeatin or the monopotassium phosphate; the technical problems of insufficient field ear number and low yield of ratooning rice in the ratooning season can be solved, and the method has good application value.

Description

Technical Field

[0001] This invention relates to the field of growth regulators for ratooning rice, specifically to a compound growth regulator that promotes seedling growth and strong panicle development in ratooning rice during the ratooning season, its preparation method, and its application. Background Technology

[0002] Ratoon rice refers to a rice cultivation model where, after the first rice harvest, dormant buds on the rice stubble sprout again to form panicles, allowing for a second harvest. This achieves "one plant, two harvests," offering significant advantages in fully utilizing temperature and light resources, improving land productivity, reducing production costs, and increasing grain yield. Developing ratoon rice is crucial for ensuring my country's food security and enhancing farmers' economic benefits. The yield of ratoon rice is highly dependent on the quality of seedling emergence and panicle formation efficiency during the ratoon season. The germination rate of regenerated buds, seedling formation rate, and panicle development directly determine the yield. Plant growth regulators, as key means of regulating the growth and development of ratoon rice, have attracted considerable attention for their application in promoting regenerated bud germination, strengthening panicles, and increasing yield.

[0003] Currently, most growth regulators used in agricultural production for controlling the ratooning season of rice are single-component formulations or simple compound formulations, mainly including gibberellins, cytokinins, and brassinolides. While gibberellins can break dormancy and promote bud elongation, thus increasing the number of ratooning seedlings to some extent, they suffer from poor targeting, easily leading to excessive vegetative growth and decreased stress resistance. Furthermore, their effect on promoting the germination of low-node axillary buds is weak, resulting in low uniformity of bud germination and insufficient seedling numbers, making it difficult to significantly increase the number of panicles in the field during the ratooning season. Cytokinins can promote cell division and bud differentiation, but when used alone, they easily cause premature senescence in the later stages, failing to provide continuous nutritional support for panicle development, leading to a low panicle formation rate. While brassinolides and other regulators can improve plant stress resistance, their effect on promoting bud germination is limited, and their use alone cannot simultaneously meet the dual needs of seedling emergence and strong panicle development.

[0004] The development of existing compound growth regulators is mostly limited to simple mixing, lacking a systematic design for the synergistic effects between components. This has significant shortcomings in improving the number of panicles and yield of ratooning rice in the field. Some compound formulations overemphasize promoting the germination of regenerated buds, resulting in excessive tillering, overly dense field populations, intense nutrient competition, and a significant decrease in the effective panicle formation rate. Other formulations focus on panicle development but inhibit the germination of lower node buds, resulting in insufficient total number of panicles in the field and restricting the effective increase in yield during the ratooning season.

[0005] Therefore, there is an urgent need to develop a compound growth regulator that can synergistically promote seedling growth and panicle development in the regeneration season of ratooning rice and increase its yield. Summary of the Invention

[0006] To address the aforementioned deficiencies in the prior art, this invention provides a compound growth regulator for promoting seedling growth and strong panicle development in ratooning rice, its preparation method, and its application. The regulator comprises the following components: zeatin nucleoside, potassium dihydrogen phosphate, a surfactant, and an alkaline solution. Zeatin nucleoside is dissolved in the alkaline solution, and an appropriate amount of water is added to achieve the target concentration. Potassium dihydrogen phosphate is then added and dissolved, followed by the surfactant. The mixture is stirred until homogeneous to prepare the compound growth regulator. The compound growth regulator is sprayed once three days before and once one day after the first harvest of ratooning rice. The dosage of the compound growth regulator is 30-50 L / mu (approximately 0.067 hectares). Spraying the compound growth regulator before and after the first harvest of ratooning rice significantly increases the number of panicles per unit area, the seed setting rate, and the yield in the ratooning season. Its effect is significantly better than applying zeatin nucleoside or potassium dihydrogen phosphate alone, and it can solve the problems of insufficient panicle number and low yield in the field during the ratooning season of ratooning rice.

[0007] To achieve the above technical effects, the following technical solution is adopted: A compound growth regulator that promotes seedling growth and strong panicle development in the regeneration season of rice comprises the following components: zeatin nucleoside, potassium dihydrogen phosphate, surfactant, and alkaline solution. Furthermore, the mass ratio of zeatin nucleoside to potassium dihydrogen phosphate is 1~2 mg: 50~80 g; Furthermore, the mass ratio of zeatin nucleoside to potassium dihydrogen phosphate is 1.6 mg: 64 g; Furthermore, the preparation method of the composite growth regulator includes the following steps: dissolving zeatin nucleoside in an alkaline solution with a concentration of 0.5~2 mol / L, adding an appropriate amount of water to the target concentration, then adding potassium dihydrogen phosphate to dissolve, and then adding a surfactant to the solution, stirring evenly to obtain the composite growth regulator solution; Furthermore, the concentration of the zeatin nucleoside in the composite growth regulator solution is 0.02~0.1 mg / L; Furthermore, the concentration of potassium dihydrogen phosphate in the composite growth regulator solution is 1~3 g / L; Furthermore, the alkaline solution is a sodium hydroxide solution with a concentration of 0.8~1.5 mol / L; Furthermore, the surfactant is Tween, and its concentration in the composite growth regulator solution is 0.5-2 g / L; Furthermore, the compound growth regulator was sprayed once three days before the first harvest of ratooning rice and once one day after the harvest. Furthermore, the dosage of the compound growth regulator is 30-50 L / mu.

[0008] The beneficial effects of this invention are as follows: This invention provides a compound growth regulator for promoting seedling emergence and panicle development in ratooning rice, its preparation method, and its application. The compound growth regulator comprises the following components: zeatin nucleoside, potassium dihydrogen phosphate, a surfactant, and an alkaline solution. Zeatin nucleoside is dissolved in the alkaline solution, and an appropriate amount of water is added to achieve the target concentration. Then, potassium dihydrogen phosphate is added and dissolved. The surfactant is then added to the solution, and the mixture is stirred until homogeneous to prepare the compound growth regulator. The compound growth regulator is sprayed once three days before and once one day after the first harvest of ratooning rice, at a dosage of 30-50 L / mu. After spraying the compound growth regulator on ratooning rice, the number of effective panicles and the grain filling rate in the field are increased by promoting axillary bud germination, ultimately increasing the yield of ratooning rice in the ratooning season. Its technical effect is significantly better than applying zeatin nucleoside or potassium dihydrogen phosphate alone. Furthermore, the combination of zeatin nucleoside and potassium dihydrogen phosphate synergistically increases the yield of ratooning rice, solving the technical problems of insufficient panicle number and low yield in the field during the ratooning season, and has good application value. Detailed Implementation

[0009] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0010] It should be noted that the following detailed descriptions are exemplary and intended to provide further illustration of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0011] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments of the present invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, and / or combinations thereof.

[0012] In the following examples, unless otherwise specified, all reagents and consumables were purchased from conventional reagent manufacturers in the art; unless otherwise specified, the experimental methods and techniques used are conventional methods and techniques in the art.

[0013] Example 1: 2 mg of zeatin nucleoside was dissolved in a 1 mol / L sodium hydroxide solution, and then water was added until the concentration of zeatin nucleoside was 0.05 mg / L. Potassium dihydrogen phosphate was then added to the solution at a concentration of 2 g / L, followed by Tween 80 at a concentration of 1 g / L. The mixture was stirred and dispersed evenly to obtain the composite growth regulator.

[0014] Example 2: 2 mg of zeatin nucleoside was dissolved in a 1 mol / L sodium hydroxide solution, and then water was added until the concentration of zeatin nucleoside was 0.1 mg / L. Potassium dihydrogen phosphate was then added to the solution at a concentration of 3 g / L, and Tween 80 at a concentration of 2 g / L was added. After stirring and dispersing evenly, the composite growth regulator was obtained.

[0015] Example 3: 2 mg of zeatin nucleoside was dissolved in a 1 mol / L sodium hydroxide solution, and then water was added until the concentration of zeatin nucleoside was 0.02 mg / L. Potassium dihydrogen phosphate was then added to the solution at a concentration of 1 g / L, and Tween 80 was added at a concentration of 0.5 g / L. After stirring and dispersing evenly, the composite growth regulator was obtained.

[0016] Comparative Example 1: 2 mg of zeatin nucleoside was dissolved in a 1 mol / L sodium hydroxide solution, and then water was added until the concentration of zeatin nucleoside was 0.05 mg / L. Tween 80 was then added to the solution at a concentration of 1 g / L. After stirring and dispersing evenly, a composite growth regulator was obtained.

[0017] Comparative Example 2: Take 40 L of water, add 80 g of potassium dihydrogen phosphate, stir until dissolved, then add 40 g of Tween 80, stir and disperse evenly to obtain a composite growth regulator.

[0018] Comparative Example 3: The blank control group was replaced with an equal amount of water instead of the compound growth regulator in Example 1.

[0019] Application examples The following field plot comparison tests were conducted on the compound growth regulators obtained in Examples 1-3 and Comparative Examples 1-3. Each case of compound growth regulator was set up with 3 plots for replication. Experimental site and field: Shihou Village, Jinjiang Town, Shanggao County, Jiangxi Province (114.90) 0 E, 28.22 0N). The experimental field was divided into 18 plots, arranged in a randomized block design, with each plot having an area of ​​70m². 2 (10m*7m), each plot is separated by field ridges and covered with plastic film to prevent water and fertilizer from mixing. To improve the applicability of this plan, mechanized harvesting is used in the first season for each plot.

[0020] The ratooning rice variety tested was Weiliangyou 8612.

[0021] Trial period: March 23, 2025 - October 28, 2025.

[0022] The experimental procedure was as follows: The first crop of ratooning rice was harvested on August 8th. Spraying experiments were conducted 3 days before and 1 day after the first crop harvest, with spraying performed after 5 PM on those days. A small sprayer was used to evenly spray the prepared compound growth regulator onto each plot. The spraying rate for each plot was calculated based on the average application rate per acre (40 L / acre). After spraying, water and fertilizer management and pest and disease control for the ratooning season were the same as for conventional planting, and remained consistent across all experimental plots. The ratooning season was harvested on October 28th. On that day, a 5m² area was randomly selected from each plot. 2 In the yield measurement area, the actual yield was calculated after manual harvesting and threshing; at the same time, 10 representative rice plants were selected along the diagonal of each plot to investigate the factors that constitute the yield.

[0023] Test method: Grain setting rate = number of filled grains / total number of grains (filled grains + empty grains).

[0024] The experimental results are shown in Table 1.

[0025] Table 1. Yield and composition of ratooning rice under different treatment groups during the ratoon season.

[0026] As shown in Table 1, compared with Comparative Examples 1-3, the treatment groups of Examples 1-3 significantly increased the number of panicles per unit area, the seed setting rate, and the actual yield during the ratooning season. Compared with Control Example 3 (blank control), the actual yield increase rate during the ratooning season in the treatment group of Example 1 was 12.78%, while the actual yield increase rates during the ratooning season in the treatment groups of Comparative Examples 1 and 2 were 3.62% and 1.7%, respectively. The yield increase rate during the ratooning season in the treatment group of Example 1 was greater than the sum of the yield increase rates of Comparative Examples 1 and 2. This invention verifies that zeatin nucleoside and potassium dihydrogen phosphate can synergistically increase the yield during the ratooning season, and their effect is significantly better than applying zeatin nucleoside or potassium dihydrogen phosphate alone. The present invention uses zeatin nucleoside and potassium dihydrogen phosphate as a compound growth regulator for ratooning rice, which can promote axillary bud germination, increase the number of effective panicles and the seed setting rate in the field during the ratooning season, and ultimately increase the yield of ratooning rice during the ratooning season. This invention can solve the technical problems of insufficient number of panicles and low yield in the field during the ratooning season of ratooning rice.

[0027] Therefore, those skilled in the art will recognize that although embodiments of the present invention have been shown and described in detail herein, many other variations or modifications conforming to the principles of the present invention can be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the invention. Therefore, the scope of the present invention should be understood and recognized as covering all such other variations or modifications.

Claims

1. A compound growth regulator that promotes seedling emergence and strong panicle growth in ratooning rice during the ratooning season, characterized in that, It includes the following components: zeatin nucleoside, potassium dihydrogen phosphate, surfactant, and alkaline solution.

2. The composite growth regulator according to claim 1, characterized in that, The mass ratio of zeatin nucleoside to potassium dihydrogen phosphate is 1~2 mg: 50~80 g.

3. The composite growth regulator according to claim 1, characterized in that, The mass ratio of zeatin nucleoside to potassium dihydrogen phosphate is 1.6 mg: 64 g.

4. The composite growth regulator according to claim 1, characterized in that, The preparation method of the composite growth regulator includes the following steps: dissolving zeatin nucleoside in an alkaline solution with a concentration of 0.5~2 mol / L, adding an appropriate amount of water to the target concentration, then adding potassium dihydrogen phosphate to dissolve, and then adding a surfactant to the solution. After stirring evenly, the composite growth regulator solution is obtained.

5. The composite growth regulator according to claim 4, characterized in that, The concentration of zeatin nucleoside in the composite growth regulator solution is 0.02~0.1 mg / L.

6. The composite growth regulator according to claim 4, characterized in that, The concentration of potassium dihydrogen phosphate in the composite growth regulator solution is 1~3 g / L.

7. The composite growth regulator according to claims 1-6, characterized in that, The alkaline solution is a sodium hydroxide solution with a concentration of 0.8 to 1.5 mol / L.

8. The composite growth regulator according to claims 1-6, characterized in that, The surfactant is Tween, and its concentration in the composite growth regulator solution is 0.5-2 g / L.

9. The application method of the compound growth regulator for promoting seedling growth and panicle development in ratooning rice according to claims 1-8, characterized in that, The compound growth regulator was sprayed once three days before the first harvest of ratooning rice and once one day after the first harvest.

10. The application method according to claim 9, characterized in that, The dosage of the compound growth regulator is 30-50 L / mu.

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