Joint detection kit for multiple virus antigens

Abstract

The invention belongs to the technical field of virus detection, and particularly discloses a multi-virus antigen joint detection kit which comprises a cylindrical kit body, a plurality of slots arranged in a circumferential array are formed in the side wall of the kit body, each slot extends towards the center of the kit body in the radial direction of the kit body, and a detection strip is movably inserted into each slot; an observation opening is formed in the position, corresponding to the detection area of the detection strip, of the top of each insertion groove, a sample adding opening is formed in the center of the kit, and a flow guide channel inclining downwards is communicated between the bottom of the sample adding opening and each insertion groove; a cut-off assembly is movably arranged at the communicating position of the flow guide channel and the inserting groove. According to the multi-virus combined screening device, samples can be accurately distributed to each detection strip, it is guaranteed that the sample introduction amount of each detection strip is uniform and consistent, the accuracy of the detection result is improved, the efficiency of multi-virus combined screening is greatly improved, after detection is completed, the intercepting assembly automatically falls to block an outlet of the flow guide channel, and residual samples are directly absorbed.

Description

Technical Field

[0001] This invention belongs to the field of virus detection technology, specifically relating to a multi-virus antigen combined detection kit. Background Technology

[0002] Rapid and accurate identification of pathogens is crucial in clinical diagnosis and public health surveillance. This is especially true for viruses transmitted through the respiratory, digestive, or bloodstream, whose initial symptoms are often similar, but whose treatment and control strategies differ significantly, such as influenza virus, respiratory syncytial virus, and adenovirus. Traditional etiological diagnosis primarily relies on methods such as virus isolation and culture, nucleic acid detection (e.g., PCR), and single pathogen antigen detection. Virus isolation and culture are time-consuming and require stringent experimental conditions; while nucleic acid detection is highly sensitive, it requires sophisticated instruments and specialized personnel, is costly, and has a relatively long result time, making it difficult to widely implement in primary healthcare institutions or on-site rapid screening. In contrast, antigen detection based on immunological principles, particularly colloidal gold or fluorescence immunochromatography, has become an important tool for point-of-care testing and early screening due to its ease of operation, speed (results typically within 15-30 minutes), lack of specialized equipment, and lower cost.

[0003] Patent CN220171052U discloses a multi-virus antigen combined detection kit, including a base plate, a top cover, and multiple test strips corresponding to different viral antigens. The base plate has multiple placement slots arranged circumferentially around its center. The test strips are placed within these slots. Each test strip includes a backing plate and, sequentially arranged on the backing plate, a sample application pad, a colloidal gold pad, a detection pad, and an absorbent pad. The detection pad has a detection line and a control line. The top cover is placed on the base plate and has multiple observation holes for the detection line and control line corresponding to the test strips. A sample application hole is located in the center of the top cover, with one end of the sample application pad for each test strip placed at the sample application hole. By combining test strips corresponding to different viruses on the same kit, multiple different viral antigens can be detected simultaneously, enabling rapid identification and convenient result viewing for testing personnel, preventing confusion. Although this patent can detect antigens of multiple different viruses on the same kit, its sample well does not have a flow splitting function, and the sample relies entirely on adsorption, which cannot quickly and accurately achieve the effect of adding the same amount of sample to different test strips. In addition, the placement slot is directly connected to the sample well, and the test strip is directly embedded in the placement slot. Sample processing is drawn in from the end of the test strip and also seeps in from the bottom of the test strip, contaminating the entire test strip. Furthermore, the residual sample after the test is completed is inconvenient to handle.

[0004] Patent CN222318961U discloses a multi-virus antigen combined detection kit, comprising a kit with several test strip mounting slots on its outer wall, each slot housing a test strip. A connecting groove is formed on the upper surface of the kit, communicating with the test strip mounting slots. A flow-splitting component, including a flow-splitting cylinder fixed within the connecting groove, is located inside the connecting groove. A connecting needle, penetrating a rubber membrane, ensures that each test strip contacts the sample inside the flow-splitting cylinder, preventing contact failures and improving the kit's detection stability. The multiple test strip mounting slots allow for the installation of multiple test strips, and the connecting needle's penetration through the rubber membrane enables multiplex detection, improving detection efficiency. While this patent uses a needle-based flow-splitting method to distribute the sample evenly to some extent, some sample inevitably remains in the needle when the test strip is removed after testing, potentially spilling and contaminating the environment.

[0005] To address the issues of uncontrollable sample loading, uneven sample distribution, and inconvenient residual sample handling in existing multi-virus antigen combination test kits, it is necessary to improve the structure of the multi-virus antigen combination test kits to solve the current technical problems. Summary of the Invention

[0006] The purpose of this invention is to provide a multi-virus antigen detection kit that can simultaneously detect multiple viruses in a single operation, significantly improving the efficiency of multi-virus joint screening. It utilizes the gravity of the detection solution itself to improve the absorption efficiency of the test strips. At the same time, it can accurately distribute the sample to each test strip, ensuring that the sample introduction volume of each test strip is uniform and consistent, thus improving the accuracy of the test results. After the test is completed, the intercepting component automatically falls down to block the outlet of the guide channel, directly absorbing the residual sample.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: a multi-virus antigen detection kit, comprising a cylindrical kit, wherein the side wall of the kit is provided with a plurality of slots arranged in a circumferential array, each slot extending radially toward the center of the kit, and a detection strip being movably inserted into each slot; Each slot has an observation port at its top corresponding to the detection area of ​​the test strip, and the reagent kit has a sample dispensing port at its center. The bottom of the sample dispensing port is connected to each slot by a downwardly sloping flow channel. A flow-blocking component is movably provided at the connection position between the flow guide channel and the slot.

[0008] By adopting the above technical solution The first step involves inserting the test strips corresponding to the specific viral antigens into the slots distributed in the circumferential array on the side wall of the reagent kit, according to the type of virus to be detected, until they are against the outlet end of the flow channel. At this point, the flow interception component is pushed upward by the test strips, completing the assembly of the detection module. The multi-slot design with a circumferential array allows for the simultaneous assembly of test strips for multiple different viral antigens, enabling simultaneous detection of multiple viruses in a single operation, significantly improving the efficiency of multi-virus joint screening.

[0009] The second step involves adding the biological sample to be tested (such as serum, throat swab eluate, etc.) to the sample dispensing port in the center of the kit. The sample flows to the corresponding test strip under its own weight and through the downward-sloping flow channel, where it undergoes a specific antigen-antibody reaction. The centralized sample dispensing port, combined with the multi-directional tilting flow channel, allows for sample distribution across multiple test strips with a single dispensing operation. This not only avoids the cumbersome process of multiple sample dispensing required in traditional multi-test methods but also reduces operational errors. The tilting flow channel design utilizes the gravity of the test solution to improve the aspiration efficiency of the test strips. Simultaneously, it ensures precise sample distribution to each test strip, guaranteeing a uniform sample volume across all strips and improving the accuracy of the test results.

[0010] The third step involves observing the color development results of each test strip through the observation port corresponding to the detection area of ​​the test strip at the top of each slot, thus achieving simultaneous detection of multiple viral antigens. Each test strip has an independent observation port, allowing for individual reading of the detection results for each viral antigen, avoiding visual confusion between results from different test strips, and facilitating rapid identification and recording.

[0011] After the test is completed, if you need to remove the test strip, simply pull it out. After the test strip is pulled out, the interception component will automatically fall down to block the outlet of the guide channel and directly absorb the remaining sample.

[0012] To better realize the present invention, the diameter of the inlet end of the slot gradually decreases from the outside to the inside.

[0013] To better realize the present invention, the diameter of the observation port gradually decreases from top to bottom.

[0014] To better realize the present invention, the bottom of the observation port near the sample application port is a smooth transition surface.

[0015] To better realize the present invention, the sample dispensing port is a funnel-shaped structure.

[0016] To better realize the present invention, a cover is detachably embedded at the top of the sample dispensing port, and a handle is fixedly connected to the center of the cover.

[0017] To better realize the present invention, a cover is fixedly embedded at the top of the sample dispensing port. The cover includes a first fixing ring and a second fixing ring concentrically disposed inside the first fixing ring. The first fixing ring is fixedly embedded at the top of the sample dispensing port, and a transparent observation window is fixedly connected between the first fixing ring and the second fixing ring. A sealing plug is detachably embedded in the second fixing ring. A handle is fixedly connected to the top of the sealing plug, and a sample funnel is fixedly connected to the bottom of the second fixing ring.

[0018] To better realize the present invention, a conical protrusion is provided at the center of the bottom surface of the sample dispensing port, and the inlets of the plurality of flow guiding channels are arranged in a circumferential array around the conical protrusion, and the outlet of the sample dispensing funnel is located directly above the conical protrusion.

[0019] To better realize the present invention, the flow interception component includes a wedge-shaped flow interception block, and the top and bottom of the slot are provided with first limiting grooves for the flow interception block to move; The flow-blocking block has an upward-opening limiting hole, and a reset spring is provided in the limiting hole. The two ends of the reset spring are fixedly connected to the flow-blocking block and the reagent kit, respectively.

[0020] To better realize the present invention, the intercepting block is fixedly connected to absorbent cotton near the flow guiding channel, and the top and bottom of the slot are provided with second limiting grooves for the absorbent cotton to move.

[0021] Beneficial effects: 1. High efficiency of multi-slot testing: The multi-slot design with a circular array can simultaneously assemble multiple test strips with different viral antigens, and can complete the simultaneous detection of multiple viruses in a single operation, which greatly improves the efficiency of multi-virus joint screening.

[0022] 2. Highly convenient to operate: The centrally located sample dispensing port, combined with the multi-directional inclined flow channel, allows for sample distribution of multiple test strips with just one sample dispensing. This not only avoids the tedious operation of traditional multi-test requiring multiple sample dispensing, but also reduces operational errors.

[0023] 3. Precise and controllable detection: The inclined flow channel design utilizes the gravity of the detection liquid itself to improve the absorption efficiency of the detection strip. At the same time, it can accurately distribute the sample to each detection strip, ensuring that the sample introduction volume of each detection strip is uniform and consistent, thereby improving the accuracy of the detection results.

[0024] 4. Clear result observation: Each test strip corresponds to an independent observation port, allowing for individual reading of the test results for each viral antigen, avoiding visual confusion between results from different test strips, and facilitating rapid identification and recording. Attached Figure Description

[0025] Figure 1This is a top view of the detection state in Embodiment 1 of the present invention; Figure 2 For the present invention Figure 1 AA cross-section diagram; Figure 3 This is a top view of Embodiment 1 of the present invention in the undetected state; Figure 4 For the present invention Figure 2 BB cross-section diagram; Figure 5 This is a three-dimensional structural diagram of Embodiment 2 of the present invention in the undetected state; Figure 6 This is a three-dimensional structural diagram of the device under the detection state in Embodiment 2 of the present invention; Figure 7 This is a top view of Embodiment 2 of the present invention in the undetected state; Figure 8 For the present invention Figure 7 CC cross-section diagram; Figure 9 This is a top view of the detection state in Embodiment 2 of the present invention; Figure 10 For the present invention Figure 9 DD cross-section diagram.

[0026] In the diagram: 1. Reagent kit; 2. Slot; 3. Detection strip; 4. Observation port; 41. Smooth transition surface; 5. Sample dispensing port; 6. Flow guide channel; 7. Flow interception assembly; 71. Flow interception block; 72. First limiting groove; 73. Limiting hole; 74. Reset spring; 75. Absorbent cotton; 76. Second limiting groove; 8. Cover; 81. First fixing ring; 82. Second fixing ring; 83. Transparent observation window; 84. Sealing plug; 9. Handle; 10. Conical protrusion. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Example like Figure 1 - Figure 4 As shown, a multi-virus antigen detection kit includes a cylindrical kit 1. The side wall of the kit 1 is provided with a plurality of slots 2 arranged in a circumferential array. Each slot 2 extends radially toward the center of the kit 1. A test strip 3 can be movably inserted into each slot 2. Each slot 2 has an observation port 4 at the top corresponding to the detection area of ​​the detection strip 3. The center of the reagent kit 1 has a sample dispensing port 5. The bottom of the sample dispensing port 5 is connected to each slot 2 by a downwardly sloping guide channel 6. A flow-blocking component 7 is movably provided at the connection position between the flow channel 6 and the slot 2.

[0029] The working principle of this invention can be summarized as follows: Step 1, test strip 3 assembly: According to the type of virus to be detected, insert the test strip 3 corresponding to the specific viral antigen into the slots 2 distributed in the circumferential array on the side wall of the reagent kit 1 until it abuts against the outlet end of the flow channel 6. At this time, the interception component 7 is pushed upward by the test strip 3, completing the assembly of the detection module.

[0030] The second step is sample loading: The biological sample to be tested (such as serum, pharyngeal swab eluate, etc.) is added to the loading port 5 in the center of the kit 1. The sample flows to the corresponding test strip 3 by its own gravity and the downward tilting flow channel 6, and undergoes an antigen-antibody specific reaction with the test strip 3.

[0031] The third step is result reading: After the reaction is completed, the color development results of each detection strip 3 are observed through the observation port 4 of the detection area corresponding to the detection strip 3 at the top of each slot 2, so as to realize the simultaneous detection of multiple viral antigens.

[0032] After the test is completed, if it is necessary to remove the test strip 3, simply pull out the test strip 3. After the test strip 3 is pulled out, the interception component 7 will automatically fall down to block the outlet of the guide channel 6 and directly absorb the residual sample.

[0033] Preferably, the diameter of the inlet end of slot 2 gradually decreases from the outside to the inside. The outwardly expanding inlet end forms a "guide structure," which reduces the alignment accuracy requirements when inserting the detection strip 3, allowing the detection strip 3 to be quickly and smoothly introduced into slot 2. Especially when assembling different detection strips 3 in batches, it significantly reduces the insertion time and operational difficulty, improving assembly efficiency. Precise positioning ensures accurate detection alignment. After the inner diameter of the inlet is reduced, it can better match the size of the detection strip 3, limiting the radial displacement of the detection strip 3. This ensures that the detection area of ​​the detection strip 3 is precisely aligned with the observation port 4, and the reaction area is precisely connected to the outlet of the guide channel 6, avoiding observation deviations or insufficient sample contact caused by the displacement of the detection strip 3.

[0034] Preferably, the diameter of the observation port 4 gradually decreases from top to bottom. By designing the observation port 4 as an outward-expanding structure, it is beneficial to provide a wider field of view, making it easier for testing personnel to quickly, clearly, and accurately read the test results.

[0035] Preferably, the bottom of the observation port 4 near the sample application port 5 is a smooth transition surface 41. This design helps protect the integrity of the test strip 3. When the test strip 3 is inserted along the slot 2, the smooth transition surface 41 replaces the sharp edges and corners, which can avoid scratching and abrasion of the test strip 3 (especially its fragile reaction / detection area), ensuring the functional integrity of the test strip 3 and preventing physical damage from affecting the test results.

[0036] Preferably, the sample dispensing port 5 has a funnel-shaped structure. Designing the sample dispensing port 5 as a funnel reduces the difficulty of sample dispensing. The larger opening area of ​​the funnel allows the sample to slide down the slope into the bottom of the port even if it is slightly off-center during dispensing, eliminating the need for precise alignment and significantly reducing the risk of spillage for non-professionals, thus improving ease of operation. It also concentrates and guides the sample, reducing residue. The narrowing bottom of the funnel quickly gathers the sample to the inlet of the flow channel 6, preventing sample residue on the inner wall of the dispensing port 5 and ensuring sufficient sample entry into the subsequent flow path, improving sample utilization and detection sufficiency. Furthermore, it prevents splashing and contamination; the wide opening and narrow bottom of the funnel buffers the liquid impact during dispensing, reducing sample splashing.

[0037] Preferably, a cover 8 is detachably embedded in the top of the sample inlet 5, and a handle 9 is fixedly connected to the center of the cover 8. Before testing, the cover 8 seals the sample inlet 5 to prevent contamination and ensure the accuracy of the test results. When a sample needs to be added, the cover 8 is opened using the handle 9 to add the sample. After testing, the cover 8 is closed again to prevent residual sample from leaking out.

[0038] Preferably, the flow-blocking assembly 7 includes a wedge-shaped flow-blocking block 71, and the top and bottom of the slot 2 are provided with first limiting grooves 72 for the flow-blocking block 71 to move; the flow-blocking block 71 is provided with an upward-opening limiting hole 73, and a return spring 74 is provided in the limiting hole 73, with both ends of the return spring 74 fixedly connected to the flow-blocking block 71 and the reagent kit 1 respectively. The wedge-shaped flow-blocking block 71 can be pushed upward when the detection strip 3 is pushed in, and under the elastic force of the return spring 74, it can also push the detection strip 3 to form a certain fixing effect. When the detection is completed and the detection strip 3 is pulled out, under the elastic force of the return spring 74, the flow-blocking block 71 moves downward, blocking the slot 2 and preventing the liquid in the guide channel 6 from flowing out.

[0039] Preferably, the intercepting block 71 is fixedly connected to an absorbent cotton 75 near the guide channel 6, and the top and bottom of the slot 2 are provided with second limiting grooves 76 for the absorbent cotton 75 to move. To improve the intercepting effect of the intercepting block 71, an absorbent cotton 75 near the guide channel 6 is fixed on the intercepting block 71. When the test strip 3 is pulled out after the test is completed, the intercepting block 71 moves down under the elastic force of the return spring 74, blocking the slot 2. The absorbent cotton 75 blocks the guide channel 6, absorbing all the sample remaining in the guide channel 6, avoiding residue in the sample inlet 5, and more effectively solving the problem of residual sample.

[0040] Example 2 like Figure 5 - Figure 10 As shown, a multi-virus antigen detection kit differs from Example 1 in that: a cover 8 is fixedly embedded at the top of the sample dispensing port 5. The cover 8 includes a first fixing ring 81 and a second fixing ring 82 concentrically disposed inside the first fixing ring 81. The first fixing ring 81 is fixedly embedded at the top of the sample dispensing port 5, and a transparent observation window 83 is fixedly connected between the first fixing ring 81 and the second fixing ring 82. A sealing plug 84 is detachably embedded inside the second fixing ring 82. A handle 9 is fixedly connected to the top of the sealing plug 84, and a sample dispensing funnel is fixedly connected to the bottom of the second fixing ring 82. A conical protrusion 10 is provided at the center of the bottom surface of the sample dispensing port 5. The inlets of multiple flow channels 6 are arranged in a circumferential array around the conical protrusion 10, and the outlet of the sample dispensing funnel is located directly above the conical protrusion 10.

[0041] This embodiment utilizes a sample dispensing funnel and a conical protrusion 10 to ensure uniform sample distribution. The outlet of the sample dispensing funnel is aligned directly above the conical protrusion 10, guiding the sample precisely onto the conical protrusion 10. The sample is then dispersed through the inclined surface of the conical protrusion 10 to the inlet of the surrounding circular array of guide channels 6, preventing localized sample accumulation at the bottom of the dispensing port 5 and ensuring that multiple guide channels 6 can uniformly acquire the sample, guaranteeing consistent sample supply to each detection strip 3. A transparent observation window 83 facilitates direct monitoring of the sample volume and flow status within the dispensing port, allowing for real-time confirmation of proper dispensing while reducing the risk of sample contamination from external exposure. The design of a sealing plug 84 and a handle 9 balances sealing and ease of operation. The sealing plug 84 is detachably embedded in the second fixing ring 82, sealing the dispensing port 5 after dispensing to prevent sample evaporation or contamination from external impurities. The top handle 9 reduces the difficulty of assembling and disassembling the sealing plug 84, making operation more effortless and efficient.

[0042] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A multi-virus antigen detection kit, characterized in that, The kit includes a cylindrical reagent kit (1), the sidewall of which is provided with a plurality of slots (2) arranged in a circumferential array, each slot (2) extending radially toward the center of the reagent kit (1), and a test strip (3) can be movably inserted into each slot (2). Each slot (2) has an observation port (4) at the top corresponding to the detection area of ​​the detection strip (3). The reagent kit (1) has a sample dispensing port (5) at the center. The bottom of the sample dispensing port (5) is connected to each slot (2) by a downwardly sloping guide channel (6). A flow-blocking component (7) is movably provided at the connection position between the flow channel (6) and the slot (2).

2. The multi-virus antigen detection kit according to claim 1, characterized in that, The diameter of the inlet end of the slot (2) gradually decreases from the outside to the inside.

3. The multi-virus antigen detection kit according to claim 1, characterized in that, The diameter of the observation port (4) gradually decreases from top to bottom.

4. The multi-virus antigen detection kit according to claim 1, characterized in that, The bottom of the observation port (4) near the sample dispensing port (5) is a smooth transition surface (41).

5. A multi-virus antigen detection kit according to claim 1, characterized in that, The sample inlet (5) has a funnel-shaped structure.

6. The multi-virus antigen detection kit according to claim 1, characterized in that, The top of the sample inlet (5) is detachably fitted with a cover (8), and a handle (9) is fixedly connected to the center of the cover (8).

7. A multi-virus antigen detection kit according to claim 1, characterized in that, The top of the sample inlet (5) is fixedly fitted with a cover (8). The cover (8) includes a first fixing ring (81) and a second fixing ring (82) concentrically arranged inside the first fixing ring (81). The first fixing ring (81) is fixedly fitted to the top of the sample inlet (5). A transparent observation window (83) is fixedly connected between the first fixing ring (81) and the second fixing ring (82). A sealing plug (84) is detachably embedded in the second fixing ring (82). A handle (9) is fixedly connected to the top of the sealing plug (84), and a sample funnel is fixedly connected to the bottom of the second fixing ring (82).

8. A multi-virus antigen detection kit according to claim 7, characterized in that, The bottom center of the sample inlet (5) is provided with a conical protrusion (10), and the inlets of the multiple flow channels (6) are arranged in a circular array around the conical protrusion (10). The outlet of the sample funnel is located directly above the conical protrusion (10).

9. A multi-virus antigen detection kit according to claim 1, characterized in that, The flow-blocking assembly (7) includes a wedge-shaped flow-blocking block (71), and the top and bottom of the slot (2) are provided with first limiting grooves (72) for the flow-blocking block (71) to move. The intercepting block (71) is provided with an upward-opening limiting hole (73), and a reset spring (74) is provided in the limiting hole (73). The two ends of the reset spring (74) are respectively fixedly connected to the intercepting block (71) and the reagent kit (1).

10. A multi-virus antigen detection kit according to claim 9, characterized in that, The intercepting block (71) is fixedly connected to the absorbent cotton (75) near the flow channel (6), and the top and bottom of the slot (2) are provided with a second limiting groove (76) for the absorbent cotton (75) to move.

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