A method for promoting mist propagation rooting of pinus sylvestris var. sylvestris

Abstract

The application provides a method for promoting mist culture rooting of Pinus squamaeformis, and relates to the technical field of Pinus squamaeformis cutting propagation. The method for promoting mist culture rooting of Pinus squamaeformis comprises the following steps: soaking Pinus squamaeformis cuttings in a citric acid solution in an ice water bath, disinfecting the cuttings, and treating the cuttings with a rooting agent; and then performing mist culture on the cuttings by adding a nutrient agent containing calcium nitrate, potassium nitrate, magnesium sulfate, copper sulfate, indole butyric acid, sodium phytate and lauryl alcohol polyether-4. The method overcomes the shortcomings of the prior art, effectively promotes rapid rooting of Pinus squamaeformis branches, guarantees the growth of root systems, provides a fast and convenient method for stable seedling raising of Pinus squamaeformis, and guarantees artificial breeding of Pinus squamaeformis.

Description

Technical Field

[0001] This invention relates to the field of Pinus sylvestris cutting propagation technology, specifically to a method for promoting root growth of Pinus sylvestris in fog culture. Background Technology

[0002] Simao pine is one of the pine trees unique to Yunnan and grows rapidly. It has excellent material quality and a wide range of uses, especially its long fiber wood, which is suitable for producing high-grade pulp and paper products. It has extremely important economic and ecological value. Therefore, in order to meet the development of the market economy, Simao pine is currently mainly planted in artificial forests.

[0003] The propagation methods of Pinus kesiya are mainly divided into sexual reproduction and asexual reproduction. Sexual reproduction is generally done by seed planting, but offspring propagated by seed are prone to phenotypic segregation, and the offspring cannot fully inherit the superior traits of the parents. Asexual reproduction is mainly done by cuttings, which can effectively ensure that the seedlings inherit the superior traits of the woody plant. However, Pinus kesiya is a species that is difficult to root by cuttings. Traditional substrate cuttings have a low overall rooting rate and require about 60-70 days to root, which is a long overall rooting time. Fog culture is a soilless seedling cultivation method in which nutrient solution is sprayed directly onto the roots in the form of a mist, which improves nutrient absorption efficiency and may promote the growth of cuttings. By adjusting the composition of the nutrient solution, the rooting of cuttings can be promoted to a certain extent. At the same time, fog culture can effectively save space. Using fog culture for Pinus kesiya seedling cultivation is an important research direction at present. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a method for promoting the rooting of Pinus simonii through mist culture. This method effectively promotes the rapid rooting of Pinus simonii branches and ensures root growth, providing a quick and convenient method for stable seedling cultivation of Pinus simonii and guaranteeing the artificial propagation of Pinus simonii.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A method for promoting root growth of Pinus simonii in fog, the method comprising the following steps: S1. Cut tender shoots of Pinus simonii and use them as branches to be processed. S2. Soak the lower 1-2cm of the branch in an ice water bath with a 5%-7.5% citric acid solution, then let it dry and cut off the lower part that was soaked in the citric acid solution, keeping the cut clean. This will give you pre-treated branch A for later use. S3. Soak the lower end of the pretreated branch A in disinfectant, rinse with clean water, soak in rooting agent, and then air dry to obtain pretreated branch B for later use. S4. Prepare the nutrient solution according to the following formula: calcium nitrate: 400-500 mg / L, potassium nitrate: 300-320 mg / L, magnesium sulfate: 120-200 mg / L, copper sulfate: 1-1.6 mg / L, indolebutyric acid: 50-100 mg / L, sodium phytate: 20-40 mg / L, lauryl ether-4: 8-16 mg / L, with the remainder being water; S5. Insert the lower end of the pretreated branch B into the hole of the opaque seedling tray, and add nutrients to the atomizer. Turn on the atomizer for 10-12 minutes every 4-6 hours to perform atomization treatment on the lower end of the pretreated branch B.

[0006] Preferably, the length of the spikelet in step S1 is 15-18 cm.

[0007] Preferably, in step S1, after cutting the scion, the pine needles at the lower 3-5cm of the scion are removed to obtain the branch to be treated.

[0008] Preferably, the lower end cut in step S2 is a flat cut.

[0009] Preferably, the ice-water bath immersion time in step S2 is 20-30 minutes.

[0010] Preferably, in step S3, the disinfectant is a 500-800 times diluted carbendazim solution, the disinfectant soaking time is 10-15 minutes, and the soaking position is 1-2 cm below the bottom of the branch.

[0011] Preferably, in step S3, the rooting agent is a 100 mg / L indoleacetic acid solution, the rooting agent soaking time is 1-2 hours, and the soaking position is 1-2 cm from the lower end of the branch.

[0012] Preferably, in step S5, when the lower end of the pretreated branch B is inserted into the hole of the opaque seedling tray, the lower end extends 1-2 cm below the hole.

[0013] Preferably, during the aeroponic treatment in step S5, the temperature is controlled at 15-28℃ and the humidity is above 80%.

[0014] This invention provides a method for promoting root growth of Pinus simonii in fog culture, which has the following advantages compared with the prior art: This invention employs a citric acid ice water bath treatment on Pinus sylvestris branches, which effectively promotes rooting of Pinus sylvestris branches in aeroponics, shortens the rooting time, and further enhances the rooting rate and number of roots by using calcium nitrate, potassium nitrate, magnesium sulfate, copper sulfate, indolebutyric acid, sodium phytate, and lauryl alcohol polyether-4 as nutrients in aeroponics. This comprehensively ensures the efficiency and effectiveness of aeroponics, achieving soilless and inorganic rooting and seedling cultivation, which can meet the needs of large-scale factory seedling production and significantly save seedling cultivation space. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the collected Pinus simaoides panicles in an embodiment of the present invention; Figure 2 This is a schematic diagram of the aeroponic treatment of Pinus simonii spikelets in an embodiment of the present invention; Figure 3 This is a schematic diagram of root growth of the aeroponic seedlings in experimental group 1 7 days after rooting in an embodiment of the present invention; Figure 4 This is a schematic diagram of root growth of the aeroponic seedlings in experimental group 2 7 days after rooting in an embodiment of the present invention; Figure 5 This is a schematic diagram of root growth of the aeroponic seedlings in experimental group 4 7 days after rooting in an embodiment of the present invention. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] Example: Simao pine mist cultivation 1. Collect tender stalks from the *Pinus simaoensis* scion orchard (retain 15-18cm, remove the lower 3-5cm of pine needles, such as...). Figure 1 (As shown), the branches to be processed are ready for use; 2. Soak the lower 1-2cm of the branch in a 6% citric acid solution in an ice water bath for 25 minutes. After that, take out the branch and let it dry. Cut off the lower part that was soaked in the citric acid solution with a flat cut. Then soak the lower 1-2cm in a 600-fold carbendazim solution for 15 minutes. Rinse it with clean water. Then soak the lower 1-2cm in a 100mg / L indoleacetic acid solution for 1.5 hours. After soaking, take out the branch and let it dry for later use. 3. Insert the lower end of the dried branch into the hole of the opaque seedling tray, extending 1-2 cm below the hole. Place the seedling tray in the center of the open-top culture box, connecting the lower end of the culture box to the atomizer. Cover the upper end with a thin film, making holes at both ends of the film (e.g., ...). Figure 2 As shown in the figure, atomization culture was carried out at a temperature of 20±2℃. During the culture period, the humidity was controlled above 80%. The nutrient solution was loaded into the atomizer, and the atomizer was turned on for 10 minutes every 5 hours.

[0018] Referring to the aeroponic method described above, different experimental groups were set up by selecting different nutrient formulations according to Table 1 below.

[0019]

[0020] Comparative Example 1: Simao pine mist cultivation The cultivation method is basically the same as in Example 1 above, except that the 6% citric acid solution ice water bath soaking treatment for 25 min in step 2 is replaced by the 6% citric acid solution room temperature soaking treatment for 25 min, and the nutrient solution is the same as that in experimental group 1.

[0021] Comparative Example 2: The cultivation method is basically the same as in Example 1 above, except for the differences in steps 1 and 2. Step 2 is as follows: 1. Collect tender scions from the Pinus simaoensis scion orchard (retain 13-16cm, remove pine needles from the bottom 1-3cm), and prepare the branches for later processing; 2. Soak the lower 1-2cm of the branch in a 6% citric acid solution in an ice water bath for 25 minutes. Then remove the branch and let it dry. Next, soak the lower 1-2cm in a 600-fold carbendazim solution for 15 minutes. Rinse it with clean water and then soak the lower 1-2cm in a 100mg / L indoleacetic acid solution for 1.5 hours. After soaking, remove the branch and let it dry for later use. Furthermore, the choice of nutrient solution was the same as that in experimental group 1.

[0022] Comparative Example 3: The cultivation method is basically the same as in Example 1 above, except for the differences in steps 1 and 2. Step 2 is as follows: 1. Collect tender scions from the Pinus simaoensis scion orchard (retain 13-16cm, remove pine needles from the bottom 1-3cm), and prepare the branches for later processing; 2. Soak the lower 1-2cm of the branch in a 600-fold carbendazim solution for 15 minutes, rinse with clean water, and then soak the lower 1-2cm in a 100mg / L indoleacetic acid solution for 1.5 hours. After soaking, take out and dry the branch for later use. Following the aeroponic method described above, the rooting rate of each group was observed and recorded at 30, 60, and 90 days after aeroponic treatment (100 cuttings were set in each group). The specific results are shown in Table 2 below:

[0023] As shown in Table 2 above, the reduction of sodium phytate and the addition of lauryl ether-4 in experimental groups 2, 3 and 4 will affect the early rooting rate of aeroponic seedlings to some extent. However, the different citric acid solution soaking methods and post-soaking treatment methods used in comparative examples 1, 2 and 3 have little impact on the final total rooting rate of aeroponic seedlings (90 days), but will significantly reduce the early rooting rate of aeroponic seedlings.

[0024] Observe and record the root length and number of roots of each group of aeroponic seedlings 7 days after rooting (defined as the appearance of white root points at the lower end of the cuttings). (Record 5 seedlings per group and calculate the average of root length and number of roots). The specific results are shown in Table 3 below:

[0025] As shown in Table 3 above, the addition of sodium phytate and lauryl ether-4 to the nutrient solution in experimental group 1 effectively promoted root growth, resulting in the aeroponic seedlings in experimental group 1 having longer root lengths and more roots in a shorter period of time. Figure 3 ), compared to experimental group 2 ( Figure 4 ) and experimental group 4 ( Figure 5 Significant progress has been made.

[0026] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for promoting root growth in *Pinus simonii* via mist cultivation, characterized in that, The method includes the following steps: S1. Cut tender shoots of Pinus simonii and use them as branches to be processed. S2. Soak the lower 1-2cm of the branch in a 5%-7.5% citric acid solution in an ice water bath, then dry it and cut off the lower part that was soaked in the citric acid solution, keeping the cut clean. This will give you pre-treated branch A for later use. The ice water bath soaking time is 20-30 minutes. S3. Soak the lower end of the pretreated branch A in disinfectant, rinse with clean water, soak in rooting agent, and then air dry to obtain pretreated branch B for later use. S4. Prepare the nutrient solution according to the following formula: calcium nitrate: 400-500 mg / L, potassium nitrate: 300-320 mg / L, magnesium sulfate: 120-200 mg / L, copper sulfate: 1-1.6 mg / L, indolebutyric acid: 50-100 mg / L, sodium phytate: 20-40 mg / L, lauryl ether-4: 8-16 mg / L, with the remainder being water; S5. Insert the lower end of the pretreated branch B into the hole of the opaque seedling tray, and add nutrients to the atomizer. Turn on the atomizer for 10-12 minutes every 4-6 hours to perform atomization treatment on the lower end of the pretreated branch B.

2. The method according to claim 1, characterized in that: In step S1, the length of the spikelet is 15-18 cm.

3. The method according to claim 1, characterized in that: In step S1, after cutting the scion, the pine needles at the lower 3-5cm of the scion are removed to obtain the branch to be treated.

4. The method according to claim 1, characterized in that: In step S2, the lower end cut is a flat cut.

5. The method according to claim 1, characterized in that: In step S3, the disinfectant is a 500-800 times diluted carbendazim solution, the disinfectant soaking time is 10-15 minutes, and the soaking position is 1-2 cm from the bottom of the branch.

6. The method according to claim 1, characterized in that: In step S3, the rooting agent is a 100 mg / L indoleacetic acid solution. The rooting agent is soaked for 1-2 hours, and the soaking position is 1-2 cm below the bottom of the branch.

7. The method according to claim 1, characterized in that: In step S5, when the lower end of the pretreated branch B is inserted into the hole of the opaque seedling tray, the lower end extends 1-2 cm below the hole.

8. The method according to claim 1, characterized in that: During the aeroponic treatment in step S5, the temperature is controlled at 15-28℃ and the humidity is above 80%.

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