Diglyceride oil containing fucoxanthine as well as preparation method and application of diglyceride oil

Abstract

The invention relates to the field of natural product extraction, in particular to fucoxanthine-containing grease as well as a preparation method and application thereof, and the method comprises the following steps: mixing a seaweed raw material with diglyceride oil, and performing extraction operation, so that fucoxanthine is extracted from the seaweed raw material, and the fucoxanthine-containing grease is obtained. Compared with traditional edible oil (such as soybean oil, olive oil and the like), the diglyceride oil is adopted as an extraction medium, the extraction efficiency of fucoxanthine in seaweed is remarkably better, the content of fucoxanthine in the diglyceride oil can be optimally increased by 50% under the same condition, and therefore the yield of target active ingredients is greatly increased, and the application prospect is wide. The method is simple in process route, only relates to physical steps such as mixing, extraction and separation, does not need to use any organic solvent, fundamentally avoids safety and environmental risks caused by solvent residue and volatilization, and meets the requirements of green production.

Description

Technical Field

[0001] This invention relates to the field of natural product extraction, and more particularly to a fucoxanthin-containing diglyceride oil, its preparation method, and its application. Background Technology

[0002] Fucoxanthin, a carotenoid naturally found in brown algae and diatoms, has attracted widespread attention for its bioactivity in recent years. Studies have shown that this pigment not only possesses significant antioxidant properties but also demonstrates potential value in anti-obesity, anti-diabetic, anti-inflammatory, cardiovascular disease prevention, and even tumor intervention. With the rapid development of the health food market, effectively integrating fucoxanthin into everyday foods or health supplements has become an important research direction in the industry. However, the inherent instability, water insolubility, and low bioavailability of fucoxanthin severely limit its application scale in actual production. These inherent characteristics lead to numerous technical bottlenecks in its extraction, storage, and human absorption. Achieving efficient, safe, and economical extraction has become a pressing challenge for the industry.

[0003] Currently, traditional methods for extracting fucoxanthin from seaweed mainly rely on organic solvents such as ethanol, methanol, ethyl acetate, or n-hexane. While these solvent extraction processes can yield fucoxanthin of a certain purity, they are typically time-consuming, energy-intensive, and involve the use of volatile organic compounds, posing safety and environmental risks. Solvent residues may also affect the food safety of the final product, which contradicts the modern food industry's pursuit of green and clean production. Therefore, developing environmentally friendly alternative extraction technologies has become a clear direction for evolution in this field.

[0004] To promote the application of fucoxanthin in food processing, some studies have explored low-cost extraction of fucoxanthin from seaweed using edible oils. Chinese invention patent CN117343030A discloses a method for preparing food-grade seaweed fucoxanthin extract. This method uses conventional edible oils (including flaxseed oil, soybean oil, rapeseed oil, and olive oil) to ultrasonically-assistedly extract fucoxanthin from seaweed, directly obtaining a fucoxanthin extract. The process is simple and environmentally friendly. However, its drawback is the poor extraction efficiency of fucoxanthin using conventional edible oils, resulting in low utilization of fucoxanthin from seaweed.

[0005] This technological bottleneck essentially points to a deeper industry need: finding a novel oil carrier that meets food-grade safety standards while possessing a high extraction capacity for fucoxanthin. While existing edible oil extraction methods have addressed the risk of solvent residue, they haven't fundamentally optimized extraction kinetics and thermodynamic processes, resulting in limited improvements in extraction efficiency and failing to meet the stringent efficiency and economic requirements of large-scale commercial production. Therefore, the industry urgently needs a completely new approach to significantly improve the efficiency of oil extraction methods without introducing chemical risks, thereby paving the way for the development of high-value-added functional oil products. Summary of the Invention

[0006] This application aims to overcome the shortcomings of existing technologies, such as low extraction efficiency, environmentally unfriendly process, and poor economic efficiency when using conventional edible oils to extract fucoxanthin. Therefore, it provides a fucoxanthin-containing diglyceride oil, its preparation method, and its application to overcome the above-mentioned deficiencies.

[0007] To achieve the above-mentioned objectives, the present invention is implemented through the following technical solution: In a first aspect, the present invention provides a method for preparing fucoxanthin-containing diglyceride oil, comprising the following steps: Seaweed raw material is mixed with diglyceride oil and extracted to extract fucoxanthin from the seaweed raw material, thereby obtaining diglyceride oil containing fucoxanthin.

[0008] As described in the background section, although the use of edible oils to replace organic solvents for extraction has made progress in terms of safety and environmental protection, for example, methods using conventional edible oils such as flaxseed oil and olive oil have been disclosed, practice has shown that the extraction efficiency of these oils for fucoxanthin is not ideal, and the valuable active ingredients in seaweed have not been fully utilized, which restricts the economic feasibility and application potential of this technology in the field of functional foods.

[0009] The applicant discovered that the key to solving this bottleneck lies in finding a food-grade oil carrier with better affinity for fucoxanthin, thereby achieving a substantial breakthrough in extraction efficiency based on green processes.

[0010] Based on insights into the aforementioned problems, this invention creatively employs diglyceride oil as a novel extraction medium. Specifically, diglyceride oil, as a safe food ingredient, has a molecular structure fundamentally different from conventional triglyceride edible oils. This structural characteristic may give it unique advantages in polarity, interfacial properties, and interaction with fucoxanthin molecules. The core of this application's technical solution lies in directly mixing seaweed raw materials, such as wakame, kelp, and sea lettuce, with diglyceride oil, and extracting it through physical methods such as stirring, ultrasound, or oscillation. Finally, a fucoxanthin-rich oil product is obtained through separation methods such as centrifugation. The key innovation of this solution lies not in the complexity of the extraction equipment or process, but in the fundamental replacement of the extraction medium itself. Its technical path is simple, easy to implement, and fully complies with the requirements of food safety production.

[0011] Experimental results show that, under essentially the same process conditions, the fucoxanthin content in oils extracted using diglyceride oil is significantly higher than that of traditional edible oils. This means that more target active substances can be extracted from the same amount of seaweed raw material, directly improving the utilization rate of raw materials and the economic efficiency of the entire production process. This significant improvement in extraction efficiency is not achieved by increasing energy consumption, extending working hours, or introducing complex steps, but rather through the optimization of the medium itself, demonstrating the high efficiency and ingenuity of the solution.

[0012] In summary, the technical solution of this invention, by introducing a novel, functionally specific extraction medium—diglyceride oil—fundamentally alters the properties of the extraction system, thereby achieving unexpected technical effects. This opens up new possibilities for developing high-value-added functional oil products containing fucoxanthin, possessing strong industrial application value and a high degree of innovation.

[0013] Preferably, the seaweed raw material is selected from at least one of wakame seaweed, kelp, or Sargassum seaweed.

[0014] Preferably, the mass ratio of the diglyceride oil to the seaweed raw material is (30:15) to (30:1.5).

[0015] Preferably, the extraction operation includes at least one of stirring, sonication, or oscillation.

[0016] Preferably, the extraction operation is carried out under heating conditions, with the heating temperature being 40°C to 70°C.

[0017] Preferably, the extraction operation takes 0.5 to 3 hours.

[0018] Preferably, the diglyceride oil contains 40% to 90% diglycerides.

[0019] Secondly, the present invention also provides an oil containing fucoxanthin. The oil contains diglyceride oil and fucoxanthin; wherein... The fucoxanthin was extracted from seaweed raw material using the method described above.

[0020] Preferably, the fucoxanthin content in the oil is between 40 ppm and 223 ppm.

[0021] Thirdly, the present invention also provides the application of the fucoxanthin-containing oil in food, health products or pharmaceuticals.

[0022] Therefore, this application has the following beneficial effects: First, by using diglyceride oil as the extraction medium, it showed significantly better extraction efficiency for fucoxanthin in seaweed compared to traditional edible oils (such as rapeseed oil, soybean oil, olive oil, etc.). Under the same conditions, the fucoxanthin content in diglyceride oil can be increased by up to 50%, thereby greatly improving the yield of the target active ingredient. Secondly, this method has a simple process route, involving only physical steps such as mixing, extraction and separation, without the use of any organic solvents, fundamentally avoiding the safety and environmental risks caused by solvent residues and volatilization, and meeting the requirements of green production; Furthermore, this method can make fuller use of seaweed raw materials, reduce the raw material cost per unit product, and has good economic benefits; Finally, the obtained product is fucoxanthin directly enriched in edible oils, which can be used as a functional ingredient in food or health product systems without complicated subsequent processing. The product form is novel and easy to use. Attached Figure Description

[0023] Figure 1 Comparison of the effects of different treatment methods on the extraction of fucoxanthin in Example 1.

[0024] Figure 2 Comparison of the extraction effects of fucoxanthin at different stirring speeds in Example 2.

[0025] Figure 3 Comparison of the extraction effects of fucoxanthin at different heating temperatures in Example 3.

[0026] Figure 4 Comparison of the extraction effects of fucoxanthin at different stirring times in Example 4.

[0027] Figure 5 Example 5: Comparison of the effects of different concentrations of diglyceride oil on the extraction of fucoxanthin.

[0028] Figure 6 Comparison of the extraction effects of fucoxanthin with different solid-liquid ratios in Example 6.

[0029] Figure 7 Comparison of the extraction effects of fucoxanthin from different seaweed raw materials in Example 7. Detailed Implementation

[0030] The present invention will be further described below with reference to specific embodiments. Those skilled in the art will be able to implement the present invention based on these descriptions. Furthermore, the embodiments of the present invention described below are generally only some, not all, of the embodiments of the present invention. Therefore, all other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort should fall within the scope of protection of the present invention.

[0031] Example 1 The purpose of Example 1 is to compare the effects of different oils and extraction methods.

[0032] The specific procedures are as follows: Weigh 3g of wakame powder and 30g of oil (olive oil, soybean oil, rapeseed oil, or diglyceride oil) into sample bottles. Extract fucoxanthin using three methods: ultrasonication (50℃, 1.5h), shaking (200r / min, 50℃, 1.5h), and stirring (300r / min, 50℃, 1.5h). After extraction, centrifuge (4000r / min, 5min) and collect the supernatant oil sample. Detect the fucoxanthin content in the oil using high-performance liquid chromatography with an ultraviolet detector. Specific experimental results are shown in Table 1 and... Figure 1 .

[0033] Table 1. Effects of different oils and processing methods on the extraction of fucoxanthin .

[0034] Example 2 The purpose of Example 2 is to compare the extraction effects of different stirring speeds.

[0035] The specific operation is as follows: Weigh 3g of wakame powder and 30g of oil (rapeseed oil and diglyceride oil) into a sample bottle. Keep the heating temperature at 50℃ and the stirring time constant for 1.5h. Extract the oil at different stirring speeds (100r / min, 150r / min, 200r / min, 250r / min, 300r / min). After extraction, centrifuge (4000r / min, 5min) and collect the upper oil sample. Detect the fucoxanthin content in the oil using high performance liquid chromatography-ultraviolet detector. The specific experimental results are shown in Table 2 and below. Figure 2 .

[0036] Table 2. Effects of different stirring speeds on the extraction of fucoxanthin .

[0037] Example 3 The purpose of Example 3 is to compare the extraction effects at different heating temperatures.

[0038] The specific operation is as follows: Weigh 3g of wakame powder and 30g of oil into a sample bottle. Keep the stirring speed constant at 300r / min and the stirring time constant at 1.5h. Extract at different heating temperatures (30℃, 40℃, 50℃, 60℃, 70℃). After extraction, centrifuge (4000r / min, 5min) and collect the upper oil sample. Detect the fucoxanthin content in the oil using high performance liquid chromatography-ultraviolet detector. The specific experimental results are shown in Table 3 and below. Figure 3 .

[0039] Table 3. Effects of different heating temperatures on the extraction of fucoxanthin .

[0040] Example 4 The purpose of Example 4 is to compare the extraction effects of different stirring times.

[0041] The specific operation is as follows: Weigh 3g of wakame powder and 30g of oil into a sample bottle. Keep the stirring speed at 300r / min and the heating temperature at 50℃ constant. Extract at different stirring times (0.5h, 1.0h, 1.5h, 2.0h, 2.5h, 3.0h). After extraction, centrifuge (4000r / min, 5min) and take the upper oil sample. Use high performance liquid chromatography-ultraviolet detector to detect the content of fucoxanthin in the oil. The specific experimental results are shown in Table 4 and below. Figure 4 After continuous stirring for 3 hours, the fucoxanthin content in the diglyceride oil increased by 50.36% compared to traditional edible oil.

[0042] Table 4. Effects of different stirring times on the extraction of fucoxanthin .

[0043] Example 5 The purpose of Example 5 is to compare the extraction effects of diglyceride oils of different concentrations.

[0044] 3g of wakame powder and 30g of oil were weighed into a sample bottle. The stirring speed was kept constant at 300 rpm, the heating temperature at 50℃, and the stirring time at 1.5 h. Extraction was performed using oils with different concentrations of diglycerides (40%, 50%, 60%, 70%, 80%, and 90% diglyceride content). After extraction, the oil was centrifuged (4000 rpm for 5 min) and the supernatant oil sample was collected. The content of fucoxanthin in the oil was detected using high-performance liquid chromatography with a UV detector. Specific experimental results are shown in Table 5. Figure 5 .

[0045] Table 5. Effects of different concentrations of diglyceride oil on fucoxanthin extraction .

[0046] Example 6 The purpose of Example 6 is to compare the extraction effects of different ratios of wakame powder to oil.

[0047] Different ratios of wakame powder and diglyceride oil were weighed into sample bottles (the ratio of wakame powder to diglyceride oil was 15:30, 12:30, 9:30, 6:30, 3:30, and 1.5:30). The stirring speed was kept constant at 300 r / min, the heating temperature at 50℃, and the stirring time at 1.5 h. Extraction was performed using the same concentration of diglyceride oil (60%). After extraction, the supernatant oil sample was collected by centrifugation (4000 r / min, 5 min). The content of fucoxanthin in the oil was detected using high-performance liquid chromatography with a UV detector. Specific experimental results are shown in Table 6. Figure 6 .

[0048] Table 6. Effects of different solid-liquid ratios on the extraction of fucoxanthin .

[0049] Example 7 The purpose of Example 7 is to compare the extraction effects of different seaweed raw materials.

[0050] 3g of different seaweed raw materials and 30g of oil were weighed into sample bottles. The stirring speed was kept constant at 300 rpm, the heating temperature at 50℃, and the stirring time at 1.5 h. After extraction, the samples were centrifuged (4000 rpm for 5 min) and the supernatant oil sample was collected. The content of fucoxanthin in the oil was detected using high-performance liquid chromatography with a UV detector. Specific experimental results are shown in Table 7. Figure 7 .

[0051] Table 7. Extraction effect of fucoxanthin from different seaweed raw materials .

[0052] The above experiments demonstrate that this invention innovatively uses diglyceride oil to replace traditional edible oil, extracting fucoxanthin from seaweed raw materials, thereby effectively increasing the fucoxanthin content in the oil. This invention is simple to operate, environmentally friendly, and has excellent extraction results. Compared with traditional edible oil, under the same conditions, the fucoxanthin content in diglyceride oil can be increased by up to 50%.

[0053] The specific embodiments described herein are merely illustrative of the spirit of the invention. Those skilled in the art to which this invention pertains may make various modifications or additions to the described specific embodiments or use similar methods to substitute them, without departing from the spirit of the invention or exceeding the scope defined by the appended claims.

Claims

1. A method for preparing fucoxanthin-containing diglyceride oil, characterized in that, Includes the following steps: Seaweed raw material is mixed with diglyceride oil and extracted to extract fucoxanthin from the seaweed raw material, thereby obtaining diglyceride oil containing fucoxanthin.

2. The method as described in claim 1, characterized in that, The seaweed raw material is selected from at least one of wakame seaweed, kelp, or Sargassum seaweed.

3. The method as described in claim 1, characterized in that, The mass ratio of the diglyceride oil to the seaweed raw material is (30:15) to (30:1.5).

4. The method as described in claim 1, characterized in that, The extraction operation includes at least one of stirring, sonication, or oscillation.

5. The method as described in claim 1 or 4, characterized in that, The extraction operation is carried out under heating conditions, with a heating temperature of 40°C to 70°C.

6. The method as described in claim 1, characterized in that, The extraction process takes 0.5 to 3 hours.

7. The method as described in claim 1, characterized in that, The diglyceride oil contains 40% to 90% diglycerides.

8. A diglyceride oil containing fucoxanthin, characterized in that, The oil contains diglyceride oil and fucoxanthin; wherein... The fucoxanthin is extracted from seaweed raw material by the method described in any one of claims 1-7.

9. The fucoxanthin-containing diglyceride oil as described in claim 8, characterized in that, The fucoxanthin content in the oil is between 40 ppm and 223 ppm.

10. The use of the fucoxanthin-containing diglyceride oil as described in claim 8 or 9 in food, health products or pharmaceuticals.

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