Application of morin

Abstract

The invention discloses application of morin, and relates to the technical field of bacterial quorum sensing inhibition. The invention discloses application of morin in preparation of a medicine for reducing enterotoxigenic escherichia coli quorum sensing. Quorum sensing of enterotoxigenic escherichia coli can be reduced by using 5.0 mg / mL of Morrin, so that secretion of virulence factors of the enterotoxigenic escherichia coli is reduced. In the Escherichia coli treated by the Morin, the Morin has no obvious inhibition effect on the growth of the Escherichia coli, and the abundance of an autoinducer 2 (AI-2) and a quorum sensing related gene is obviously reduced, so that the toxicity and the adhesion capability of the Escherichia coli are reduced. The method for reducing enterotoxigenic Escherichia coli quorum sensing provided by the invention is beneficial to replacing traditional antibiotic in-vivo treatment and assists in resisting bacterial infection.

Description

Technical Field

[0001] This invention relates to the field of bacterial quorum sensing inhibition technology, and more specifically to the application of morin in the preparation of drugs that reduce quorum sensing of enterotoxigenic Escherichia coli. Background Technology

[0002] Based on their pathogenicity, *Escherichia coli* can be classified into enterotoxigenic *E. coli* (ETEC), enterohemorrhagic *E. coli* (EHEC), enteropathogenic *E. coli* (EPEC), enteroadhesive *E. coli* (EAEC), enteroinvasive *E. coli* (EIEC), and diffusely adherent *E. coli* (DAEC). ETEC is particularly prone to infecting young animals, causing diarrhea and even death, and is a common zoonotic pathogen with a high mortality rate. Quorum sensing (QS) is a cell-to-cell communication mechanism based on chemical signaling molecules within microbial populations. Its core lies in the synthesis, release, and sensing of signaling molecules such as quorum sensing molecules (QSM), which regulate population gene expression and functional behavior according to population density. Morin (3,5,7,2',4'-pentahydroxyflavone) is a natural active substance isolated from plants, belonging to the flavonoid class of compounds. It is a secondary metabolite of phenols in plants and is widely distributed in nature. Morin has good antioxidant and anti-inflammatory effects.

[0003] Currently, there has been some progress in the study of the pathogenic mechanism of enterotoxigenic Escherichia coli, but the inhibitory function of linphedra in enterotoxigenic Escherichia coli has not yet been reported. Summary of the Invention

[0004] In view of this, the present invention provides the application of linalool in the preparation of drugs that reduce quorum sensing of enterotoxigenic Escherichia coli.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] Application of linalool in the preparation of drugs that reduce quorum sensing of enterotoxin-producing Escherichia coli.

[0007] Furthermore, the linalool is used to reduce the concentration of self-inducer-2 in enterotoxigenic Escherichia coli. Alternatively, reduce the abundance of genes related to quorum sensing in enterotoxin-producing Escherichia coli. Or reduce the virulence of enterotoxin-producing Escherichia coli. Or reduce the inhibitory and adhesion ability of enterotoxigenic Escherichia coli to IPEC-J2 activity.

[0008] A composition for reducing quorum sensing of enterotoxigenic Escherichia coli, the composition comprising linalool, a medically acceptable carrier.

[0009] Furthermore, the concentration of morin in the composition is 1.0~10.0 mg / mL.

[0010] Furthermore, the concentration of morin in the composition is 5.0 mg / mL.

[0011] As can be seen from the above technical solution, compared with the prior art, the beneficial effects of the present invention are as follows: In this invention, linalool does not significantly inhibit the growth of enterotoxigenic Escherichia coli, but it significantly inhibits quorum sensing. Furthermore, by reducing quorum sensing, it decreases the secretion of virulence and adhesion factors, showing great potential for application in practical defense. The method for reducing quorum sensing of enterotoxigenic Escherichia coli provided by this invention is beneficial for controlling bacterial infections and meets the requirements of green environmental protection.

[0012] Using 5.0 mg / mL Morin can reduce quorum sensing in enterotoxigenic Escherichia coli, thereby reducing the secretion of its virulence factors. In Morin-treated E. coli, Morin had no significant inhibitory effect on E. coli growth, but significantly reduced the abundance of its autoinducer-2 (AI-2) and quorum sensing-related genes, thus reducing its virulence and adhesion ability. The method for reducing quorum sensing in enterotoxigenic E. coli provided by this invention is beneficial as an alternative to traditional in vivo antibiotic therapy, helping to combat bacterial infections. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0014] Figure 1 The image shows the antibacterial effect of linphelodes extract on enterotoxin-producing Escherichia coli. Figure 2 The effect of morin on the concentration of autoinducer-2 in enterotoxin-producing Escherichia coli and the abundance of quorum sensing-related genes is shown in the figure. Figure 3 The graph shows how linpogonin reduces the toxicity of ETEC and decreases the inhibitory effect of ETEC on IPEC-J2 activity. Figure 4 A graph showing the ability of morin to reduce the adhesion of ETEC to IPEC-J2 activity. Detailed Implementation

[0015] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0016] Example 1 Phellinus linteus reduces quorum sensing in enterotoxin-producing Escherichia coli Oxford cup inhibition zone assay was performed on enterotoxigenic Escherichia coli using antibiotics and five concentrations of morin (0.0 mg / mL, 2.5 mg / mL, 5.0 mg / mL, 10.0 mg / mL, and 20.0 mg / mL). Enterotoxigenic Escherichia coli was cultured with the five concentrations of morin in LB broth at 37°C, and bacterial count was determined by dilution and plating every 2 hours. ETEC cultured with 5.0 mg / mL Morin was collected after reaching the logarithmic growth phase for subsequent analysis. The concentration of quorum sensing autoinducer-2 was detected by ELISA. Changes in the abundance of quorum sensing-related genes, virulence factor-related genes, and adhesion factor-related genes in enterotoxigenic Escherichia coli were detected by qPCR. 1×10⁶ cells were treated with 5.0 mg / mL Morin. 6 After 4 hours of ETEC treatment with CFU / mL, the supernatant was collected and used to treat adherent IPEC-J2 cells for 4 hours. Cell viability was then assessed using MTT assay to evaluate ETEC toxicity. 1×10⁶ cells were treated with 5.0 mg / mL Morin. 6 After CFU / mL mRFP1-ETEC for 4 h, IPEC-J2 cells were treated for 4 h, and then ETEC adhesion ability was observed after staining with rabbit anti-antigen.

[0017] The results are as follows Figure 1 As shown, linalool had no significant antibacterial effect against enterotoxin-producing Escherichia coli, and did not significantly inhibit bacterial growth; for example Figure 2 As shown, linalool significantly reduced the concentration of autoinducer-2 and the abundance of quorum sensing-related genes in enterotoxigenic Escherichia coli; Figure 3 and 4 As shown, 5.0 mg / mL Morin reduces the toxicity of ETEC and decreases its inhibitory and adhesive abilities against IPEC-J2. In summary, 5.0 mg / mL Morin reduces the toxicity and adhesive abilities of enterotoxigenic Escherichia coli by decreasing quorum sensing.

[0018] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. Application of linalool in reducing quorum sensing in enterotoxin-producing Escherichia coli.

2. The application according to claim 1, characterized in that, The linpheline is used to reduce the concentration of autoinducer-2 in enterotoxigenic Escherichia coli. Alternatively, reduce the abundance of genes related to quorum sensing in enterotoxin-producing Escherichia coli. Or reduce the virulence of enterotoxin-producing Escherichia coli. Or reduce the inhibitory and adhesion ability of enterotoxigenic Escherichia coli to IPEC-J2 activity.

3. A composition for reducing quorum sensing of enterotoxigenic Escherichia coli, characterized in that, The composition includes linalool, a medically acceptable carrier.

4. The composition according to claim 3, characterized in that, The concentration of morin in the composition is 1.0~10.0 mg / mL.

5. The composition according to claim 4, characterized in that, The concentration of morin in the composition is 5.0 mg / mL.

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