A ginseng forest frog oil composition for reducing the dryness of ginseng and application thereof

Abstract

This invention relates to the field of traditional Chinese medicine composition technology, and in particular to a ginseng-frog oil composition for reducing the dryness of ginseng and its application. The frog oil in the composition provided by this invention can significantly reduce the dryness caused by ginseng alone, specifically by reducing the levels of serum inflammatory factors (IL-6, TNF-α), malondialdehyde (MDA), and cortisol (CORT), increasing superoxide dismutase (SOD) activity, reducing saliva secretion, and decreasing animal thermoresponsiveness. This effectively solves the technical pain points of significant dryness caused by ginseng alone and its limited long-term application, achieving a "nourishing without drying" effect. The composition provided by this invention has high safety and reproducibility, consists of only two natural components, requires no additional excipients, and can be widely used in the preparation of mild tonic health products, pharmaceuticals, and functional foods, possessing good application prospects and promotional value.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine composition technology, and in particular to a ginseng frog oil composition for reducing the dryness of ginseng and its application. Background Technology

[0002] Ginseng, a traditional and precious Chinese medicinal herb, is sweet, slightly bitter, and slightly warm in nature. It belongs to the spleen, lung, heart, and kidney meridians and has the effects of greatly replenishing vital energy, tonifying the spleen and lungs, promoting body fluid production and nourishing blood, and calming the mind and improving intelligence. Modern pharmacological research shows that ginseng is rich in active ingredients such as ginsenosides, which can regulate metabolism and enhance immune function, making it a commonly used tonic in clinical practice and daily health maintenance. However, due to its slightly warm nature, ginseng, when used alone or in excessive doses, can easily trigger a "dryness" reaction, specifically manifested as increased inflammatory response, aggravated oxidative stress, and fluid depletion. In severe cases, it can lead to symptoms related to "ginseng abuse syndrome," such as elevated blood pressure, nosebleeds, and mental agitation, greatly limiting its long-term application and promotion. This is also the core technical challenge facing ginseng as a tonic.

[0003] In existing technologies, methods to reduce the dryness of ginseng mainly include combining ginseng with yin-nourishing herbs such as Ophiopogon japonicus and Anemarrhena asphodeloides, or using herbs like snow pear and honey for moistening. These methods either require the addition of multiple excipients, increasing the complexity and cost of the preparation process, or they can only alleviate some of the dryness and fail to maintain the tonic effects of ginseng itself, thus exhibiting significant limitations. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a ginseng-frog oil composition for reducing the dryness of ginseng and its application. The frog oil in the composition provided by this invention can significantly reduce the dryness caused by ginseng alone, effectively solving the technical pain points of significant dryness and limited long-term application of ginseng alone, achieving a "nourishing without drying" effect.

[0005] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a ginseng and frog oil composition for reducing the dryness of ginseng, comprising ginseng and frog oil, wherein the ginseng is dried ginseng and / or ginseng water extract; and the mass ratio of the ginseng to the frog oil is 1300~1500:330~380 based on the mass of the dried ginseng and the dried frog oil.

[0006] Preferably, the mass ratio of ginseng to frog oil is 1390~1400:350~360.

[0007] Preferably, the mass ratio of ginseng to frog oil is 1395:352.

[0008] Preferably, the ginseng includes artificially cultivated ginseng that has been grown for 3 to 5 years; the forest frog oil includes dried oviducts of the Northeast Forest Frog.

[0009] The present invention provides the application of the ginseng frog oil composition described above in the preparation of drugs having one or more of the following effects, including: reducing the dryness of ginseng, anti-fatigue, anti-cold and hypoxia tolerance.

[0010] Preferably, the reduction of ginseng's dryness includes one or more of the following: reducing serum inflammatory factor levels, reducing serum malondialdehyde levels, reducing serum cortisol levels, increasing superoxide dismutase activity, increasing saliva secretion, increasing total body water content, and lowering body temperature.

[0011] Preferably, the inflammatory factors include IL-6 and / or TNF-α.

[0012] Preferably, the dosage form of the drug includes powder, capsule, oral liquid, granules or tablets.

[0013] This invention provides the application of the ginseng frog oil composition described above in the preparation of health products that relieve physical fatigue and / or improve hypoxia tolerance.

[0014] Preferably, the dosage form of the health product includes powder, capsule, oral liquid, granules or tablets.

[0015] Beneficial effects: This invention provides a ginseng-frog oil composition for reducing the dryness of ginseng, comprising ginseng and frog oil, wherein the ginseng is dried ginseng and / or ginseng water extract; the mass ratio of ginseng to frog oil is 1300-1500:330-380 based on the mass of dried ginseng and dried frog oil. The frog oil in the composition provided by this invention can significantly reduce the dryness caused by ginseng alone, specifically by reducing the levels of inflammatory factors (IL-6, TNF-α), malondialdehyde (MDA), and cortisol (CORT) in serum, increasing superoxide dismutase (SOD) activity, reducing saliva secretion, and decreasing animal thermoresponsiveness. This effectively solves the technical pain points of significant dryness and limited long-term application of ginseng alone, achieving a "nourishing without drying" effect. The composition provided by this invention has high safety and reproducibility, consists of only two natural components, requires no additional excipients, and can be widely used in the preparation of mild tonic health products, pharmaceuticals, and functional foods, possessing good application prospects and promotional value. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0017] Figure 1 This is the result of an infrared thermal imaging scan; Figure 2 The results of the observation of the oral cavity status of rats; Figure 3 3D response surface plot of human ginseng and frog oil formulation on the cold resistance-hypoxia tolerance comprehensive score of mice; Figure 4 Contour plot of the combined cold resistance and hypoxia tolerance scores of mice with human ginseng and frog oil. Detailed Implementation

[0018] This invention provides a ginseng and frog oil composition for reducing the dryness of ginseng, comprising ginseng and frog oil, wherein the ginseng is dried ginseng and / or ginseng water extract; and the mass ratio of the ginseng to the frog oil is 1300~1500:330~380 based on the mass of the dried ginseng and the dried frog oil.

[0019] In one embodiment, the mass ratio of ginseng to frog oil is 1390~1400:350~360. In another embodiment, the mass ratio of ginseng to frog oil is 1395:352.

[0020] In one embodiment, the ginseng includes artificially cultivated ginseng that has been grown for 3-5 years, with stable ginsenoside content, definite efficacy, and relatively mild drying properties; the frog oil includes dried oviducts of the Northeast Forest Frog. In one embodiment, the frog oil is frog oil powder with a particle size of less than 80-120 mesh.

[0021] As one embodiment, the preparation method of the ginseng water extract includes: taking dried ginseng, washing it with purified water, draining it, cutting it into 1-2 cm slices, adding 8 times the amount of purified water, letting it stand for 2 hours, then decocting it for 1.5 hours, and filtering it; adding 6 times the amount of purified water to the remaining residue and continuing to decoct it for 2 hours, repeating this process twice; combining all the decoctions, drying them, making them into powder, passing them through an 80-120 mesh sieve, and taking the sieve-passing material as the ginseng water extract.

[0022] The composition provided by this invention represents the optimal ratio of ginseng and frog oil. The combination of these two ingredients exhibits a significant synergistic effect in combating fatigue, cold, and hypoxia. Its overall anti-stress effect is superior to that of single ingredients or other combinations. This invention overcomes the technical shortcomings of existing technologies, such as the significant drying effect of ginseng when used alone, the limited long-term application, and the numerous excipients, complex processes, and poor efficacy of existing methods for reducing dryness. It effectively reduces the drying effect of ginseng while fully preserving its tonic properties, perfectly aligning with the traditional Chinese medicine principle of "counteracting opposites to counteract," achieving a "tonifying without drying" effect. No additional excipients are required, significantly improving the safety and applicability of ginseng. This composition consists only of two natural components, ginseng and frog oil, without any chemical additives. It is highly safe, has no obvious toxic side effects, and is highly reproducible. It can be widely used in the preparation of health products, pharmaceuticals, and functional foods related to anti-fatigue, cold, and hypoxia resistance, possessing excellent application prospects and promotional value.

[0023] The preparation method of the composition provided by the present invention is simple, requiring only the ginseng and frog oil to be crushed separately and mixed in proportion, without the need for complicated processing.

[0024] The composition provided by this invention can be widely used in the preparation of mild tonic health products, pharmaceuticals and functional foods. It is suitable for people with weak constitutions who need to take ginseng for a long time but are worried about dryness reactions, as well as people working in cold environments, people adapting to high-altitude environments, and people with weak constitutions. It meets the tonic needs of modern people and has good application prospects and promotion value.

[0025] Based on the above advantages, the present invention provides the application of the ginseng frog oil composition described in the above technical solution in the preparation of drugs with one or more of the following effects, including: reducing the dryness of ginseng, anti-fatigue, anti-cold and hypoxia tolerance.

[0026] As one implementation method, reducing the dryness of ginseng includes one or more of the following: reducing serum inflammatory factor levels, reducing serum malondialdehyde levels, reducing serum cortisol levels, increasing superoxide dismutase activity, increasing saliva secretion, increasing total body water content, and lowering body temperature. As one implementation method, the inflammatory factors include IL-6 and / or TNF-α.

[0027] As one implementation, the dosage form of the drug includes powder, capsule, oral liquid, granules or tablets.

[0028] Based on the above advantages, the present invention provides the application of the ginseng frog oil composition described in the above technical solution in the preparation of health products that relieve physical fatigue and / or improve hypoxia tolerance.

[0029] As one implementation method, the dosage form of the health product includes powder, capsule, oral liquid, granules or tablets.

[0030] To further illustrate the present invention, the following detailed description, in conjunction with embodiments and accompanying drawings, provides a ginseng frog oil composition for reducing the dryness of ginseng and its application, but these descriptions should not be construed as limiting the scope of protection of the present invention.

[0031] Preparation Example Preparation of ginseng water extract powder: Select 5-year-old artificially cultivated ginseng, wash it with purified water, drain it, cut it into 1-2cm slices, add 8 times the weight of purified water, let it stand for 2 hours, then decoct for 1.5 hours and filter it; add 6 times the weight of purified water to the remaining residue and continue to decoct for 2 hours, repeating twice; combine all the decoctions, dry them, make them into powder, pass them through an 80-mesh sieve, and collect the sieve residue for later use.

[0032] Preparation of frog oil powder: Take frog oil, freeze dry at low temperature, pulverize, pass through an 80-mesh sieve, and collect the sieve residue for later use.

[0033] Preparation of Ophiopogon japonicus water extract powder: Wash Ophiopogon japonicus with purified water, drain, add 8 times the mass of purified water, let stand for 2 hours, decoct for 1.5 hours, and filter; add 6 times the mass of purified water to the remaining residue and continue to decoct for 2 hours, repeat twice; combine all decoctions, dry, make into powder, pass through an 80-mesh sieve, and collect the sieve residue for later use.

[0034] Example 1 A ginseng and frog oil composition, comprising ginseng powder and frog oil powder, wherein the mass ratio of ginseng powder to frog oil powder is 1395:352.

[0035] Ingredients: Ginseng (five-year-old artificially cultivated), frog oil (dried oviduct of Northeast forest frog).

[0036] Preparation method: Five-year-old artificially cultivated ginseng was washed, dried, pulverized, and passed through an 80-mesh sieve to obtain ginseng powder. The frog oil was washed, dried, pulverized, and passed through an 80-mesh sieve to prepare frog oil powder. Weigh out ginseng powder and frog oil powder according to the specified ratio, mix them evenly, and you will get the ginseng and frog oil composition.

[0037] This powder is convenient to take; it can be dissolved in water and taken directly, and it does not cause significant dryness.

[0038] Example 2 The powdered composition prepared in Example 1 was mixed with starch as a filler, and then filled using a capsule filling machine to form capsules. Each capsule contains 139.5 mg of ginseng and 35.2 mg of frog oil. These capsules are convenient to take and carry, avoid the irritation of the gastrointestinal tract caused by the drying properties of ginseng, and are suitable for long-term use.

[0039] Example 3 Take 139.5 g of ginseng and 35.2 g of frog oil, add 10 times their weight of purified water, heat to 80℃ and extract twice, each extraction time 1.5 hours. Combine the two extracts, filter and concentrate, add sweetener for flavoring, fill into 10 ml / vial, sterilize to obtain the oral liquid composition. Each 10 ml contains the equivalent of 139.5 mg of ginseng and 35.2 mg of frog oil in crude form. This oral liquid has a mild taste and no obvious dryness, suitable for all types of people, especially those with difficulty swallowing.

[0040] Example 4 1. Experimental Design 1.1 Experimental Animals: SD rats, weighing 180-220g, with half males and half females, were randomly divided into experimental group, ginseng-only group, frog oil-only group, blank control group, and ginseng + Ophiopogon japonicus control group, with 10 rats in each group. All rats were acclimatized for 3 days, with free access to water and food. The rearing environment was controlled at a temperature of 22±2℃, humidity of 50±5%, and a light cycle of 12h / 12h to ensure consistent animal condition and reduce experimental error.

[0041] 1.2 Experimental grouping and administration method: Experimental group: Ginseng 1395 mg / kg + frog oil 352 mg / kg was administered. The prepared ginseng water extract powder and frog oil powder were mixed with distilled water in a certain proportion and administered by gavage once a day. The dosage was 10 mL / kg body weight each time. That is, based on the original drug (crude drug), 10 mL contains 1395 mg of ginseng and 352 mg of frog oil. The same applies to the next group. Ginseng monotherapy group: Ginseng water extract powder was administered by gavage at a dose of 1395 mg / kg once daily, with each dose being 10 mL / kg body weight, to verify the dryness reaction of ginseng monotherapy. Frog oil monotherapy group: Frog oil powder was administered by gavage at a dose of 352 mg / kg once daily, with each dose being 10 mL / kg body weight, to verify that frog oil itself is non-drying and has the potential to moisturize. Blank control group: Administered an equal volume of physiological saline once daily at a dose of 10 mL / kg body weight as a blank control. Ginseng + Ophiopogon japonicus control group: Ginseng water extract powder 1395 mg / kg + Ophiopogon japonicus powder 352 mg / kg were administered by gavage once a day, with each dose being 10 mL / kg body weight, to compare the beneficial effects of the dryness-reducing and efficacy-enhancing experimental group.

[0042] 1.3 Administration Cycle: Continuous gavage for 30 days simulates the long-term application of ginseng, better reflecting the drying properties of ginseng and the moisturizing effect of frog oil, thus meeting practical application needs. During the administration period, the general condition of the rats was closely observed, including their mental state, food and water intake, and coat condition, and dryness-related behaviors were recorded in detail.

[0043] 1.4 Sample Collection and Indicator Detection: After drug administration, a comprehensive score was obtained using a dryness rating scale (Table 1). In the final experiment, rats were fasted for 12 hours, and blood samples were collected. Serum was separated after centrifugation at 3000 r / min for 10 min. Enzyme-linked immunosorbent assay (ELISA) was used to detect dryness-related indicators in the serum, specifically including inflammatory factors (IL-6, TNF-α), oxidative stress indicators (MDA, SOD), and HPA axis-related indicators (cortisol CORT). Saliva secretion in rats was measured using the pilocarpine stimulation method. Body fluid composition was analyzed using a body composition analyzer. Infrared thermal imaging was used to detect changes in rat body temperature and the distribution of body heat. ImageJ image processing software was used to calculate the heat percentage. The results are shown in Table 1. Figure 1 .

[0044] Table 1 Comprehensive Scoring Table for Dryness Performance

[0045] 1.5 Statistical Methods: SPSS 26.0 software was used for statistical analysis. Measurement data are expressed as mean ± standard deviation (x ± s). One-way ANOVA was used for comparisons between groups. P A value <0.05 indicates a statistically significant difference, which conforms to the standard practice of statistical analysis of biological experimental data.

[0046] 2. Experimental Results Table 2. Results of serum dryness index detection in different groups

[0047] Note: Compared with the blank control group, the levels of IL-6, TNF-α, MDA, and CORT in the ginseng monotherapy group were significantly increased. P <0.05%, SOD activity was significantly reduced ( P <0.05), and the rats exhibited typical signs of dryness such as increased water intake, dry fur, and hyperactivity, confirming that ginseng alone has a significant dryness-inducing effect; compared with the ginseng alone group, the experimental group showed significant improvement in all the above-mentioned dryness-related indicators (#). P The value was <0.05), and there was no significant difference compared with the blank control group and the frog oil-only group. The rats showed no obvious signs of dryness, confirming that the combination of frog oil and ginseng can effectively reduce the dryness of ginseng.

[0048] Table 3. Results of detection of total body fluid water content, saliva secretion, body temperature, and heat distribution in different groups.

[0049] Note: Compared with the blank control group, the total water content and saliva secretion in the ginseng-only group were significantly reduced. P <0.05); body temperature, total calorie percentage, and comprehensive score of dryness symptoms were significantly increased ( P <0.05), and the rats exhibited typical signs of dryness such as increased water intake and dry fur, confirming that ginseng alone has a significant drying effect; compared with the ginseng alone group, the experimental group showed improvement in all the above-mentioned dryness-related indicators (#). P The value was <0.05, and there was no significant difference compared with the blank control group and the frog oil-only group, confirming that frog oil and ginseng can effectively reduce the dryness of ginseng.

[0050] The results of oral cavity condition observation are as follows Figure 2 .

[0051] The results showed that, compared with the blank control group, the serum levels of inflammatory factors IL-6 and TNF-α in rats treated with ginseng alone were significantly increased, the level of oxidative stress marker MDA was significantly increased, the activity of SOD was significantly decreased, and the level of HPA axis-related marker cortisol (CORT) was significantly increased. P <0.01), and the rats exhibited typical signs of dryness such as increased water intake, dry fur, and hyperactivity, confirming that ginseng alone has significant dryness-causing properties, which is also a major technical challenge in the application of ginseng; compared with the ginseng-only group, the experimental group showed significant improvement in all the above-mentioned dryness-related indicators. P <0.01, and there was no significant difference compared with the blank control group and the frog oil-only group ( P >0.05), and the rats showed no obvious signs of dryness, fully demonstrating that frog oil can significantly alleviate the dryness of ginseng. The combination of the two achieves the technical effect of "nourishing without causing dryness". Compared with existing techniques for reducing the dryness of ginseng (such as the combination of ginseng and Ophiopogon japonicus), the dryness-reducing effect of this invention is more obvious, and the combination is simple and the preparation is convenient.

[0052] Example 5 1. Experimental Design 1.1 Experimental Animals: Kunming mice, weighing 18-22g, with half males and half females, were randomly divided into experimental group, ginseng-only group, frog oil-only group, blank control group, and ginseng-ophiopogon japonicus control group, with 20 mice in each group. All mice were acclimatized for 3 days, with free access to water and food. The rearing environment was controlled at a temperature of 22±2℃, humidity of 50±5%, and a light cycle of 12h / 12h to ensure consistent animal condition and reduce experimental error.

[0053] 1.2 Experimental grouping and administration method: Experimental group: Ginseng 1395 mg / kg + frog oil 352 mg / kg were administered by gavage. The ginseng water extract powder and frog oil powder were mixed in proportion using distilled water and administered once daily. The dosage was 0.2 mL / 10g body weight (20 mL / kg body weight). That is, based on the original drug (crude drug), 20 mL contained 1395 mg of ginseng and 352 mg of frog oil. The same principle applies to the next group. Ginseng monotherapy group: Ginseng powder was administered by gavage at a dose of 1395 mg / kg once daily, with each dose being 0.2 mL / 10 g body weight; Frog oil monotherapy group: Frog oil powder was administered by gavage at a dose of 352 mg / kg once daily, with each dose being 0.2 mL / 10 g body weight; Blank control group: Administered an equal volume of physiological saline once daily, with each dose being 0.2 mL / 10 g body weight.

[0054] The ginseng and ophiopogon control group was given 1395 mg / kg of ginseng and 352 mg / kg of ophiopogon, once a day, with each dose being 0.2 mL / 10g body weight. 1.3 Dosage cycle: 30 consecutive days of oral administration to simulate the long-term application scenario of ginseng, which better reflects the drying properties of ginseng and the moisturizing effect of frog oil, and meets the actual application needs.

[0055] 1.4 Sample Collection and Index Detection: After drug administration, 10 mice from each group were subjected to a normobaric closed hypoxia tolerance test. The remaining 10 mice from each group were assessed using a dryness rating scale (Table 1), and an anti-fatigue test was conducted using a water turbine fatigue tester. After the anti-fatigue test, blood samples were collected, and serum was separated after centrifugation at 3000 r / min for 10 min. Enzyme-linked immunosorbent assay (ELISA) was used to detect dryness-related indicators in the serum, specifically including inflammatory factors (IL-6, TNF-α), oxidative stress indicators (MDA, SOD), and HPA axis-related indicators (cortisol CORT).

[0056] 1.5 Statistical methods: SPSS 26.0 software was used for statistical analysis. Quantitative data were expressed as mean ± standard deviation (x ± s). One-way ANOVA was used for comparisons between groups. A p < 0.05 was considered statistically significant, which conforms to the standard practice of statistical analysis of biological experimental data.

[0057] 2. Experimental Results Table 4. Results of serum dryness index detection on the compatibility of ginseng and frog oil.

[0058] Note: Compared with the blank control group, the levels of IL-6, TNF-α, MDA, and CORT in the ginseng monotherapy group were significantly increased. P The concentration of SOD was significantly reduced (P<0.05), and the mice exhibited typical signs of dryness, such as increased water intake, dry fur, and hyperactivity, confirming that ginseng alone has a significant dryness-inducing effect. Compared with the ginseng-only group, the experimental group showed significant improvement in all the above-mentioned dryness-related indicators (#). P The value was <0.05), and there was no significant difference compared with the blank control group and the frog oil-only group. The mice did not show obvious signs of dryness, which confirms that frog oil can effectively reduce the dryness of ginseng.

[0059] Table 5. Test results of fatigue resistance, hypoxia tolerance, and dryness reduction in different groups.

[0060] Note: Compared with the blank control group, the experimental group, the ginseng-only group, and the frog oil-only group all showed significantly increased anti-fatigue and hypoxia tolerance survival time. P <0.05); Compared with the ginseng-only group and the frog oil-only group, the experimental group showed significantly increased anti-fatigue and hypoxia tolerance survival time (#). P <0.05), confirming that ginseng, when combined with frog oil, has a significant synergistic effect in anti-fatigue and hypoxia tolerance.

[0061] 3. Analysis of Experimental Results Experimental results showed that long-term continuous administration of ginseng alone (1395 mg / kg, 30 days) significantly increased the levels of inflammatory factors IL-6 and TNF-α in the serum of mice, significantly increased the level of oxidative stress indicator MDA, significantly decreased SOD activity, and significantly increased the level of HPA axis-related indicator cortisol (CORT). Simultaneously, mice exhibited dryness-related behaviors such as increased water intake, dry hair, and hyperactivity, consistent with existing literature reports on the dryness-related effects of ginseng. This further confirms that ginseng alone has significant dryness-related effects, which is a major technical challenge in the application of ginseng for health maintenance.

[0062] After 30 days of continuous administration of ginseng (1395 mg / kg) combined with frog oil (352 mg / kg), the aforementioned dryness-related indicators in mice significantly decreased, showing no significant difference compared to the blank control group and the frog oil-only group. Furthermore, the mice were in good general condition with no obvious signs of dryness, fully demonstrating that frog oil can significantly alleviate the dryness of ginseng. The combination of the two achieves the technical effect of "nourishing without causing dryness." Compared with existing techniques for reducing ginseng dryness (such as the combination of ginseng and Ophiopogon japonicus), the dryness-reducing effect of this invention is more significant (the average comprehensive dryness index of ginseng and frog oil is 0.7±0.1, while that of ginseng and Ophiopogon japonicus is 1.29±0.2). The combination of ginseng and frog oil reduces the average dryness by approximately 45.74% compared to the combination of ginseng and Ophiopogon japonicus. Moreover, the combination of ginseng and frog oil can enhance the anti-fatigue and hypoxia tolerance of ginseng, achieving the superior effect of reducing dryness and enhancing efficacy. The composition provided by this invention is simple to formulate and easy to prepare, possessing significant innovation.

[0063] Example 6 1. Experimental Design 1.1 Experimental Animals: Kunming mice, weighing 18-22g, with half males and half females, were randomly divided into an experimental group, a ginseng-only group, a frog oil-only group, and a blank control group, with 20 mice in each group. All mice were acclimatized for 3 days, with free access to water and food. The rearing environment was controlled at a temperature of 22±2℃, humidity of 50±5%, and a light cycle of 12h / 12h to ensure consistent animal condition and reduce experimental error.

[0064] 1.2 The administration method for the experimental groups was the same as in Example 5.

[0065] 1.3 Administration cycle: Administer by gavage for 7 consecutive days, in accordance with the conventional usage cycle of cold-resistant and hypoxia-resistant products, to ensure full efficacy.

[0066] 1.4 Experimental Methods: (1) Cold resistance experiment: After the administration of the drug, the mice were placed in a low temperature environment of -18℃ and the survival time (min) of the mice was recorded. The survival time was used as the core indicator for evaluating the cold resistance effect. (2) Hypoxia tolerance test: After the administration of the drug, the mice were placed in a sealed wide-mouth bottle (one mouse per bottle) and the survival time (s) of the mice was recorded. The survival time was used as the core indicator for evaluating the hypoxia tolerance effect.

[0067] 1.5 Statistical methods: SPSS 26.0 software was used for statistical analysis. Quantitative data were expressed as mean ± standard deviation (x ± s). One-way ANOVA was used for comparisons between groups. A p < 0.05 was considered statistically significant, which conforms to the standard practice of statistical analysis of biological experimental data.

[0068] 2. Experimental Results Table 6. Results of cold resistance survival time and hypoxia tolerance survival time in different groups.

[0069] Note: Compared with the blank control group, the experimental group, the ginseng-only group, and the frog oil-only group all showed significantly increased cold resistance and hypoxia tolerance survival time. P <0.05); Compared with the ginseng-only group and the frog oil-only group, the experimental group showed significantly increased cold resistance and hypoxia tolerance survival time (# P <0.05), confirming that ginseng, when combined with frog oil, has a significant synergistic effect in resisting cold and hypoxia.

[0070] 3. Analysis of Experimental Results The experimental results showed that the survival time of mice in the experimental group against cold and the survival time against hypoxia were significantly longer than those in the ginseng-only group, the frog oil-only group, and the blank control group. P <0.05). Among them, the survival time of the experimental group against cold was increased by 21.06% compared with the ginseng-only group and by 14.02% compared with the frog oil-only group; the survival time against hypoxia was increased by 14.55% compared with the ginseng-only group and by 20.24% compared with the frog oil-only group, which fully confirms that in the optimal ratio composition determined by the present invention, ginseng and frog oil have a significant synergistic effect in resisting cold and hypoxia.

[0071] Pharmacological mechanism analysis shows that ginseng can enhance the body's resistance to cold and hypoxia by regulating metabolism and improving microcirculation; frog oil can help enhance the body's resistance to cold and hypoxia by supplementing the body's required nutrients and enhancing physiological functions. When the two are combined in the optimal ratio of this invention, their advantages complement each other, resulting in a synergistic effect.

[0072] Example 7 1. Experimental Design: Following the method in Example 6, only experimental groups were set up, with two factors (ginseng dosage A and frog oil dosage B), each factor having three levels, for a total of nine groups of compatibility experiments. The mice were given the corresponding dosage of ginseng and frog oil compound, and the cold resistance survival time (min) and hypoxia tolerance survival time (s) of mice in each group were measured. Six replicate samples were set up for each group to ensure the reliability of the experimental data.

[0073] Table 7 Experimental Groups and Raw Data

[0074] 2. Data Standardization and Comprehensive Scoring: The range standardization method (0-1 interval) was used to eliminate the dimensional differences between the two indicators of cold resistance and hypoxia tolerance. The formula is as follows: di = (Xi - Xmin) / (Xmax - Xmin); Where Xi is the mean of each group of indicators, Xmin is the minimum value of the indicator, and Xmax is the maximum value of the indicator.

[0075] The overall score Y = 0.5d1 + 0.5d2 is calculated with each factor weighted at 50% (d1 is the standardized value for cold resistance, and d2 is the standardized value for hypoxia tolerance).

[0076] 3. Mathematical Model Fitting: Using ginseng dosage A and frog oil dosage B as independent variables, and the comprehensive score Y as the dependent variable, a bivariate quadratic regression model is established: Ŷ = b0 + b1A + b2B + b11A2 + b22B2 + b12AB. The regression equation is obtained by fitting the model using the least squares method. Ŷ=0.639-0.081A+0.018B-0.349A2-0.015B2-0.178AB.

[0077] 4. Optimal Formulation Determination: A comprehensive evaluation was conducted using both cold-resistance survival time and hypoxia tolerance survival time as dual indicators. After range standardization, a comprehensive score was calculated with equal weights. A bivariate quadratic polynomial regression model was used to fit the equation, yielding a comprehensive score regression equation. The partial derivative of the model was calculated and set to zero, resulting in the optimal solution for the coding level: A = 0.517 and B = -2.475. This translates to actual dosages of 1390–1400 mg / kg for ginseng and 350–354 mg / kg for frog oil. The preferred formulation is 1395 mg / kg ginseng + 352 mg / kg frog oil. This formulation exhibits the strongest synergistic effect in cold resistance and hypoxia tolerance, resulting in the highest comprehensive score.

[0078] The 3D response surface plot of the effect of ginseng and frog oil on the combined cold resistance and hypoxia tolerance score of mice is shown in the figure. Figure 3 The contour plot of the effect of ginseng and frog oil on the combined cold resistance and hypoxia tolerance scores of mice is shown below. Figure 4 .

[0079] Depend on Figure 3 It can be seen that the 3D response surface has a single-peak convex shape, with obvious peak regions within the experimental dose range and no obvious saddle points, indicating that the combination of ginseng and frog oil has a significant dose-synergistic effect and optimal range of action on the comprehensive anti-stress ability of mice; the peak region corresponds to ginseng 1390~1400 mg / kg and frog oil 350~354 mg / kg, which is consistent with the optimal ratio determined in this invention, confirming the scientificity and reliability of the ratio optimization.

[0080] Depend on Figure 4 As can be seen, the contour lines are distributed in an elliptical shape, with the central point (ginseng 1395 mg / kg, frog oil 352 mg / kg) being the area with the highest comprehensive score. The density of the surrounding contour lines gradually decreases, indicating that the optimal ratio area is concentrated and has good stability. This further verifies the scientific nature of the optimal ratio of the present invention and provides intuitive experimental support for the inventiveness of the patent.

[0081] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A ginseng frog oil composition for reducing the dryness of ginseng, characterized in that, It includes ginseng and frog oil, wherein the ginseng is dried ginseng and / or ginseng water extract; and the mass ratio of the ginseng and frog oil is 1300~1500:330~380 based on the mass of the dried ginseng and the dried frog oil.

2. The ginseng frog oil composition according to claim 1, characterized in that, The mass ratio of ginseng to frog oil is 1390~1400:350~360.

3. The ginseng frog oil composition according to claim 2, characterized in that, The mass ratio of ginseng to frog oil is 1395:

352.

4. The ginseng frog oil composition according to any one of claims 1 to 3, characterized in that, The ginseng mentioned includes artificially cultivated ginseng that has been grown for 3 to 5 years; the forest frog oil includes dried oviducts of the Northeast Forest Frog.

5. The use of the ginseng frog oil composition according to any one of claims 1 to 4 in the preparation of a medicament having one or more of the following effects, comprising: It reduces the dryness of ginseng, combats fatigue, resists cold, and improves tolerance to hypoxia.

6. The application according to claim 5, characterized in that, The reduction of ginseng's dryness includes one or more of the following: reducing serum inflammatory factor levels, reducing serum malondialdehyde levels, reducing serum cortisol levels, increasing superoxide dismutase activity, increasing saliva secretion, increasing total body water content, and lowering body temperature.

7. The application according to claim 6, characterized in that, The inflammatory factors include IL-6 and / or TNF-α.

8. The application according to claim 5, characterized in that, The dosage forms of the drug include powder, capsules, oral liquid, granules or tablets.

9. The use of the ginseng frog oil composition according to any one of claims 1 to 4 in the preparation of health products that relieve physical fatigue and / or improve hypoxia tolerance.

10. The application according to claim 9, characterized in that, The dosage forms of the health products include powder, capsules, oral liquid, granules or tablets.

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