Experimental device for simulating plant waterlogging stress

By introducing a combination of a turbine and cleaning rotor water circulation system, as well as aeration pipes and nitrogen pipes, into the plant waterlogging stress experimental device, the problems of root exudate accumulation and difficulty in controlling dissolved oxygen caused by stagnant water were solved. This enabled precise control of water circulation and dissolved oxygen, improving the reliability and repeatability of experimental data.

CN122004065APending Publication Date: 2026-05-12HARBIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN UNIV
Filing Date
2026-04-02
Publication Date
2026-05-12

AI Technical Summary

Technical Problem

Existing plant waterlogging stress experimental devices use stagnant water, which leads to the accumulation of root exudates and anaerobic fermentation by microorganisms. This makes it impossible to achieve water circulation and renewal and precise control of dissolved oxygen levels, resulting in uneven experimental conditions and poor data reliability and repeatability.

Method used

A water circulation system including a turbine and a cleaning rotor is adopted, combined with an aeration pipe and a nitrogen pipe, to achieve precise control of water circulation and dissolved oxygen. The turbine and cleaning rotor are driven by a motor to achieve water circulation, and the aeration pipe and nitrogen pipe are used to simulate flood environments with different degrees of oxygen deficiency.

Benefits of technology

It improves the accuracy and repeatability of experimental data, reduces the accumulation of root exudates, ensures smooth water circulation, simulates diverse waterlogging environments, and reduces experimental errors.

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Abstract

The invention discloses an experimental device for simulating plant waterlogging stress, and relates to the technical field of plant experiments.The technical scheme includes that the experimental device comprises an experimental tank body, anti-vortex heads are fixedly connected to the front end and the rear end of the experimental tank body, circulating pipes are fixedly connected to the upper portions of the anti-vortex heads, and turbines are rotatably connected into the circulating pipes located at the front end of the experimental tank body; the upper part of the turbine is fixedly connected with a third gear, the third gear is in meshed connection with a second gear, the front end of the experiment tank body is provided with a first motor, and the output end of the first motor is provided with the second gear. The turbine rotates to drive the water body in the experiment tank body to flow and circulate, the circulation structure continuously updates the water body in the root zone and reduces enrichment of harmful substances, in the circulation process, the vortex-preventing head and the filter screen arranged on the two sides of the experiment tank body can prevent vortex from being generated to affect plants, and meanwhile impurities in the water body are filtered out.
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Description

Technical Field

[0001] This invention relates to the field of plant stress resistance physiology experimental technology, and in particular to an experimental device for simulating waterlogging stress in plants. Background Technology

[0002] Plant waterlogging stress is an important direction in plant stress resistance physiology research. To conduct plant waterlogging stress experiments, it is necessary to stably and accurately reproduce the natural flood environment, so as to provide standardized experimental conditions for plant waterlogging tolerance identification, physiological response mechanism exploration and stress-resistant variety screening. At the same time, the experimental device should be able to accurately control the flooding depth, duration and degree of hypoxia, simulate different stress intensities such as field waterlogging and total flooding, and be suitable for potted and small plant cultivation, so as to meet the needs of long-term continuous control experiments and multi-dimensional index measurement.

[0003] Currently, most existing plant waterlogging stress experimental devices use still water to simulate waterlogging environments. Still water easily leads to the accumulation of plant root exudates and anaerobic fermentation by microorganisms, resulting in root blackening and rotting. Furthermore, existing devices cannot achieve water circulation and renewal, and it is difficult to accurately control the dissolved oxygen level in the water, making it impossible to simulate waterlogging environments with different degrees of oxygen deficiency. This results in uneven experimental conditions, large environmental errors, and poor reliability and repeatability of experimental data, failing to meet the precision requirements for plant waterlogging stress mechanism research and waterlogging-tolerant variety evaluation.

[0004] To address the aforementioned technical problems, this invention proposes an experimental device for simulating waterlogging stress in plants. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of existing plant waterlogging stress experimental devices, which use stagnant water, leading to the accumulation of root exudates and anaerobic fermentation by microorganisms, resulting in poor experimental results. Furthermore, these devices cannot achieve water circulation and renewal, and it is difficult to precisely control the dissolved oxygen content in the water. This invention provides an experimental device for simulating plant waterlogging stress. This device can achieve water circulation and renewal, while precisely controlling the dissolved oxygen content in the water, simulating waterlogging environments with different degrees of oxygen deficiency, and improving the accuracy and repeatability of experimental data.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: An experimental apparatus simulating waterlogging stress in plants includes an experimental tank. Anti-vortex heads are fixedly connected to both the front and rear ends of the experimental tank. A circulation pipe is fixedly connected to the upper part of each anti-vortex head. A turbine is rotatably connected inside the circulation pipe at the front end of the experimental tank. A third gear is fixedly connected to the upper part of the turbine, and the third gear meshes with a second gear. A first motor is fixedly installed at the front end of the experimental tank. The output end of the first motor is fixedly connected to the second gear. A reducer is connected to the end of the second gear away from the first motor. A transmission assembly is provided at the output end of the reducer. A cleaning rod, rotatably connected to the anti-vortex head, is fixedly connected to the lower part of the transmission assembly. A cleaning head, which fits against the outer surface of the anti-vortex head, is fixedly connected to the upper part of the cleaning rod. The first motor drives the turbine to rotate through the second and third gears, realizing water circulation within the experimental tank. Simultaneously, the reducer drives the cleaning rod to rotate through the transmission assembly, achieving automatic cleaning of the anti-vortex head and ensuring smooth water circulation.

[0007] The technical solution of the present invention also includes the following preferred structural features: 1. The transmission assembly includes a first transmission wheel fixedly connected to the output end of the reducer. The first transmission wheel is connected to a second transmission wheel via a transmission belt. The second transmission wheel is fixedly connected to the upper part of the cleaning rotating rod. Through the transmission cooperation of the first transmission wheel, the transmission belt, and the second transmission wheel, the power of the reducer is stably transmitted to the cleaning rotating rod.

[0008] 2. A filter screen is fixedly connected to the outside of the water inlet end of the anti-vortex head. The filter screen is used to filter impurities in the water entering the circulation pipe to prevent impurities from entering the turbine and affecting its normal operation.

[0009] 3. Aeration pipes and nitrogen pipes are fixedly connected to the inner walls on both sides of the experimental tank. The output ends of the aeration pipes and nitrogen pipes are connected to the air inlet head, which is fixedly connected to the inner wall of the experimental tank. The aeration pipes are used to aerate and supply oxygen to the water, and the nitrogen pipes are used to introduce nitrogen into the water to create an oxygen-deficient environment. The air inlet head provides a channel for the gas to enter the experimental tank.

[0010] 4. A regulating valve is provided at the connection between the air inlet head and the aeration pipe and the nitrogen pipe. A guide shell is fixedly connected to one end of the air inlet head inside the experimental tank. The regulating valve is used to regulate the gas flow and flow rate of the aeration pipe and the nitrogen pipe. The guide shell is used to guide the airflow entering the experimental tank so that the gas is evenly distributed in the water.

[0011] 5. A second motor is fixedly installed on the inner wall of the regulating valve. The output end of the second motor is fixedly connected to a first gear that is rotatably connected to the inner wall of the regulating valve. Both sides of the first gear are meshed with transmission racks that are slidably connected to the inner wall of the regulating valve. A connecting rod is fixedly connected to the end of the transmission rack away from the first gear. A movable baffle that is slidably connected to the inner wall of the regulating valve is fixedly connected to the end of the connecting rod away from the transmission rack. The movable baffle is adapted to the outlet of the aeration pipe and the nitrogen pipe.

[0012] 6. The second motor drives the connecting rod and the movable baffle to slide through the meshing of the first gear and the transmission rack, thereby achieving precise control of the gas flow rate of the aeration pipe and the nitrogen pipe, and thus realizing the gradient adjustment of the dissolved oxygen content in the water.

[0013] The present invention has the following beneficial effects: 1. This invention achieves an integrated design of water circulation and automatic filter cleaning by linking the first motor with the turbine and the cleaning rod. This not only refreshes the water in the root zone to reduce the accumulation of harmful substances, but also avoids filter clogging, ensuring smooth water circulation and improving the long-term operational stability of the device. 2. This invention uses an aeration pipe and a nitrogen pipe in conjunction with an electric regulating valve to achieve gradient control of dissolved oxygen in water, which can simulate different flood environments with saturated dissolved oxygen, meet diverse experimental needs, and improve the reliability of experimental data. 3. The anti-vortex head and guide shell design of the present invention respectively avoids the vortex disturbance of water circulation and the root erosion caused by gas introduction, ensuring the stability of the plant growth environment and reducing experimental errors. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of an experimental device for simulating waterlogging stress in plants, as proposed in this invention. Figure 2 This is a top view of the overall structure of the device in this invention; Figure 3 This is a schematic diagram of the air intake head connection relationship in this invention; Figure 4 This is a schematic diagram of the internal structure of the regulating valve in this invention; Figure 5 This is a schematic diagram of the internal structure of the circulation pipe in this invention; Figure 6 This is a schematic diagram of the anti-vortex head connection relationship in this invention.

[0015] In the diagram: 1. Experimental tank; 2. Anti-vortex head; 3. Circulation pipe; 4. First motor; 5. Guide shell; 6. Air inlet; 7. Regulating valve; 8. Aeration pipe; 9. Nitrogen pipe; 10. Filter screen; 11. Second motor; 12. First gear; 13. Transmission rack; 14. Connecting rod; 15. Movable baffle; 16. Turbine; 17. Second gear; 18. Third gear; 19. Reducer; 20. First transmission wheel; 21. Transmission belt; 22. Second transmission wheel; 23. Cleaning rod; 24. Cleaning head. Detailed Implementation

[0016] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] Example 1 like Figures 1-6 As shown, an experimental apparatus simulating waterlogging stress in plants includes an experimental tank 1. Anti-vortex heads 2 are fixedly connected to both the front and rear ends of the experimental tank 1. A circulation pipe 3 is fixedly connected to the upper part of each anti-vortex head 2. A turbine 16 is rotatably connected inside the circulation pipe 3 located at the front end of the experimental tank 1. A third gear 18 is fixedly connected to the upper part of the turbine 16, and the third gear 18 meshes with a second gear 17. A first motor 4 is fixedly installed at the front end of the experimental tank 1. The output end of the first motor 4 meshes with the second gear 17. The gear 17 is fixedly connected, and the end of the second gear 17 away from the first motor 4 is connected to a reducer 19. The output end of the reducer 19 is fixedly connected to a first transmission wheel 20. The first transmission wheel 20 is connected to the second transmission wheel 22 via a transmission belt 21. The second transmission wheel 22 is fixedly connected to the upper part of the cleaning rod 23. The cleaning rod 23 is rotatably connected to the anti-vortex head 2, and the upper part of the cleaning rod 23 is fixedly connected to a cleaning head 24. A filter screen 10 is fixedly connected to the outer side of the water inlet end of the anti-vortex head 2.

[0018] During the experiment, the first motor 4 is started to drive the second gear 17 to rotate. The rotation of the second gear 17 drives the turbine 16 to rotate, and the rotation of the turbine 16 causes the water inside the experimental tank 1 to circulate. During the water circulation, the second gear 17 drives the transmission component to rotate, which in turn drives the cleaning rod 23 to rotate. This allows the surface of the anti-vortex head 2 to be cleaned simultaneously during the water circulation, effectively ensuring the water circulation effect. The first motor 4 drives the second gear 17, the third gear 18, and the turbine 16 to achieve water circulation in the experimental tank 1. At the same time, the reducer 19 and the transmission component drive the cleaning rod 23 to move, achieving automatic cleaning of the anti-vortex head 2.

[0019] The transmission assembly includes a first transmission wheel 20 located at the output end of the reducer 19. The first transmission wheel 20 is connected to a transmission belt 21, and the transmission belt 21 is connected to a second transmission wheel 22. The second transmission wheel 22 is fixedly connected to a cleaning rod 23, which is rotatably connected to the anti-vortex head 2. The first transmission wheel 20, the transmission belt 21, and the second transmission wheel 22 form a transmission structure that transmits the power of the reducer 19 to the cleaning rod 23, enabling the cleaning rod 23 to rotate stably. A filter screen 10 is fixedly connected to the outside of the anti-vortex head 2. The filter screen 10 filters the water that enters the circulation pipe 3 during water circulation, reducing the entry of impurities and ensuring the normal operation of the turbine 16.

[0020] In this embodiment, the experimental tank 1 is adapted for the cultivation of potted plants and small plants. During the experiment, the plants are planted inside the experimental tank 1. By adding water to the experimental tank 1 at different heights, the simulation control of different degrees of waterlogging stress is achieved. The first motor 4 is started, and its output end drives the second gear 17 to rotate. The second gear 17 drives the third gear 18 to rotate through meshing, which in turn drives the turbine 16 to rotate in the circulation pipe 3. The rotation of the turbine 16 drives the water in the experimental tank 1 to form a circulating flow, realizing the continuous renewal of the water in the root zone, reducing the accumulation of harmful substances such as root exudates and anaerobic metabolites, ensuring that the stress source of the plants in the experiment is only the waterlogging environment, and improving the authenticity of the experimental results.

[0021] During water circulation, the anti-vortex head 2 effectively prevents the formation of vortices at the circulation inlet, avoiding vortex disturbance to plant roots and affecting experimental results. The filter screen 10 filters the water entering the circulation pipe 3, reducing impurities in the water from entering the turbine 16 and ensuring the normal operation of the turbine 16. At the same time, when the second gear 17 rotates, it drives the reducer 19 to rotate synchronously. The output end of the reducer 19 drives the first transmission wheel 20 to rotate. The first transmission wheel 20 drives the second transmission wheel 22 to rotate through the transmission belt 21, which in turn drives the cleaning rod 23 to rotate around the anti-vortex head 2. The cleaning head 24 on the cleaning rod 23 rotates synchronously with it, cleaning the surface of the filter screen 10, effectively preventing impurities from accumulating on the surface of the filter screen 10 and ensuring the passage of water circulation.

[0022] Example 2 like Figures 1-6 As shown, aeration pipes 8 and nitrogen pipes 9 are fixedly connected to the inner walls of both sides of the experimental tank 1. The output ends of aeration pipes 8 and nitrogen pipes 9 are connected to air inlets 6. Air inlets 6 are fixedly connected to the inner wall of the experimental tank 1, and regulating valves 7 are provided at the connection points between air inlets 6 and aeration pipes 8 and nitrogen pipes 9. A guide shell 5 is fixedly connected to one end of air inlets 6 inside the experimental tank 1. A second motor 11 is fixedly installed on the inner wall of the regulating valve 7. A first gear 12 is fixedly connected to the output end of the second motor 11. A transmission rack 13 is meshed on both sides of the first gear 12. The end of the transmission rack 13 away from the first gear 12 is fixedly connected to a movable baffle 15 through a connecting rod 14. The transmission rack 13 and the movable baffle 15 are slidably connected to the inner wall of the regulating valve 7, and the movable baffle 15 is adapted to the discharge ports of aeration pipes 8 and nitrogen pipes 9.

[0023] In this embodiment, air is introduced into the water in the experimental tank 1 through the aeration pipe 8, which can increase the dissolved oxygen content of the water and simulate the oxygen-rich environment of mild flooding; nitrogen is introduced into the water through the nitrogen pipe 9, which can expel the dissolved oxygen in the water and reduce the dissolved oxygen content of the water, simulating the hypoxic environment of moderate and severe flooding. The opening of the aeration pipe 8 or the nitrogen pipe 9 can be controlled separately according to the experimental requirements, so as to realize the gradient control and dynamic change simulation of the dissolved oxygen content of the water and meet the experimental requirements of flood stress of different hypoxia levels.

[0024] During gas introduction, the guide shell 5 at the front end of the air inlet 6 guides the airflow, allowing the gas to diffuse evenly in the water and preventing the gas from directly scouring the plant roots and causing mechanical damage. The second motor 11 is started, and its output end drives the first gear 12 to rotate. The first gear 12 drives the transmission racks 13 on both sides to slide along the inner wall of the regulating valve 7 through meshing. The transmission racks 13 drive the movable baffle 15 to slide synchronously through the connecting rod 14. By adjusting the overlapping area of ​​the movable baffle 15 with the outlet of the aeration pipe 8 and the nitrogen pipe 9, the gas flow rate can be precisely controlled, realizing the fine regulation of dissolved oxygen in the water. The adjustment accuracy is high, effectively improving the controllability of experimental conditions.

[0025] Although embodiments of the present invention have been shown and described, those skilled in the art will be able to make various changes, modifications, substitutions and variations to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An experimental apparatus for simulating waterlogging stress in plants, comprising an experimental tank (1), characterized in that, Anti-vortex heads (2) are fixedly connected to both the front and rear ends of the experimental tank (1). A circulation pipe (3) is fixedly connected to the upper part of the anti-vortex head (2). A turbine (16) is rotatably connected inside the circulation pipe (3) located at the front end of the experimental tank (1). A third gear (18) is fixedly connected to the upper part of the turbine (16). The third gear (18) meshes with the second gear (17). A first motor (4) is provided at the front end of the experimental tank (1). The output end of the first motor (4) is connected to the second gear (17). The second gear (17) is fixedly connected to a reducer (19) on one end away from the first motor (4). The output end of the reducer (19) is provided with a transmission assembly. The lower part of the transmission assembly is fixedly connected to a cleaning rod (23). The upper part of the cleaning rod (23) is fixedly connected to a cleaning head (24). The cleaning rod (23) is rotatably connected to the anti-vortex head (2). The cleaning head (24) is a flexible cleaning brush. Its bristles are closely attached to the surface of the filter screen (10) on the outside of the anti-vortex head (2).

2. The experimental apparatus for simulating waterlogging stress in plants according to claim 1, characterized in that, The transmission assembly includes a first transmission wheel (20) fixedly connected to the output end of the reducer (19), the first transmission wheel (20) being connected to a second transmission wheel (22) via a transmission belt (21), and the second transmission wheel (22) being fixedly connected to the upper part of the cleaning rod (23).

3. The experimental apparatus for simulating waterlogging stress in plants according to claim 1, characterized in that, A filter screen (10) is fixedly connected to the outside of the water inlet end of the anti-vortex head (2).

4. The experimental apparatus for simulating waterlogging stress in plants according to claim 1, characterized in that, Aeration pipes (8) and nitrogen pipes (9) are fixedly connected to the inner walls of both sides of the experimental tank (1). Several sets of aeration pipes (8) and nitrogen pipes (9) are fixedly connected to the inner walls of both sides of the experimental tank (1). Each set of aeration pipes (8) and nitrogen pipes (9) is provided with an air inlet (6) on the side close to the experimental tank (1). The air inlet (6) is fixedly connected to the inner wall of the experimental tank (1). The output ends of the aeration pipes (8) and nitrogen pipes (9) are connected to the air inlet (6).

5. The experimental apparatus for simulating waterlogging stress in plants according to claim 4, characterized in that, A regulating valve (7) is provided at the connection between the air inlet (6) and the aeration pipe (8) and the nitrogen pipe (9). A guide shell (5) is fixedly connected to one end of the air inlet (6) inside the experimental tank (1).

6. The experimental apparatus for simulating waterlogging stress in plants according to claim 5, characterized in that, A second motor (11) is fixedly installed on the inner wall of the regulating valve (7). The output end of the second motor (11) is fixedly connected to a first gear (12). The first gear (12) is rotatably connected to the inner wall of the regulating valve (7) through a bearing.

7. The experimental apparatus for simulating waterlogging stress in plants according to claim 6, characterized in that, Both sides of the first gear (12) are meshed with a transmission rack (13), the transmission rack (13) is slidably connected to the inner wall of the regulating valve (7), and a connecting rod (14) is fixedly connected to the end of the transmission rack (13) away from the first gear (12).

8. The experimental apparatus for simulating waterlogging stress in plants according to claim 7, characterized in that, A movable baffle (15) is fixedly connected to one end of the connecting rod (14) away from the transmission rack (13). The movable baffle (15) is slidably connected to the inner wall of the regulating valve (7), and the movable baffle (15) is adapted to the outlet of the aeration pipe (8) and the nitrogen pipe (9).