Stereoscopic fluorescence tomography device

Abstract

The invention relates to a three-dimensional fluorescence tomography imaging device, which comprises a shooting part, an image processing part, a plurality of exciting light emitting parts, a tomography light emitting part, a first reflecting mirror, a beam splitter, a second reflecting mirror, a multi-wavelength light source part and a wavelength adjustable light filter, the image processing unit extracts a first separated image corresponding to the first fluorescent developer and a second separated image corresponding to the second fluorescent developer from each of the fluorescent images, and calculates depths corresponding to each of the first separated image and the second separated image based on depth information in the optical coherence tomography image. And the first separated images and the second separated images are combined according to the depths of the first separated images and the second separated images, so that a three-dimensional fluorescent tomographic image corresponding to the first fluorescent developer and a three-dimensional fluorescent tomographic image corresponding to the second fluorescent developer of the shot object are formed respectively.

Description

Technical Field

[0001] This invention relates to a stereofluorescence tomography device, specifically to a stereofluorescence tomography device capable of simultaneously acquiring fluorescence images and optical coherence tomography images of the same subject to form a stereofluorescence tomography image. Background Technology

[0002] With the development of medical technology, numerous medical imaging techniques have emerged, including fluorescence imaging, optical coherence tomography (OCT), CT, MRI, and ultrasound. Different medical imaging techniques have their own advantages and specific applications. Therefore, to gain a comprehensive understanding of a patient's condition, doctors typically employ multiple medical imaging techniques to conduct a thorough examination of the affected area, making a comprehensive judgment on the condition and obtaining an accurate diagnosis.

[0003] Fluorescence imaging utilizes the autofluorescent substances present in the human body or pre-injected fluorescent contrast agents to clearly display tissues, organs, lesions, etc., that are invisible through other medical imaging techniques. Typically, the images obtained through fluorescence imaging are planar images. To obtain three-dimensional fluorescence images, the sample needs to be scanned layer by layer, which is cumbersome, time-consuming, and cannot be completed quickly or in real time.

[0004] Therefore, there is an urgent need for a device that can obtain stereofluorescence tomography images in real time. Summary of the Invention

[0005] Technical issues

[0006] One object of the present invention is to provide a stereofluorescence tomography device that can automatically adjust the field of view of multiple excitation beams according to the distance from the subject being photographed, so that the field of view of the multiple excitation beams overlaps on the subject being photographed and completely covers the subject being photographed.

[0007] One object of the present invention is to provide a stereofluorescence tomography device that can simultaneously obtain fluorescence images and optical coherence tomography images, and combine the fluorescence images into stereofluorescence tomography images based on the depth information in the optical coherence tomography images.

[0008] One object of the present invention is to provide a stereofluorescence tomography device that can simultaneously obtain fluorescence images and optical coherence tomography images, and combine fluorescence images corresponding to different types of autofluorescent substances and / or fluorescent developers into stereofluorescence tomography images based on depth information in the optical coherence tomography images.

[0009] The technical problems that this invention aims to solve are not limited to those described above. Those skilled in the art can understand other technical problems that this invention aims to solve based on the description in this specification.

[0010] Technical solution

[0011] This invention provides a stereofluorescence tomography imaging device, comprising: an imaging unit for imaging a subject to obtain a fluorescence image and an optical coherence tomography image; an image processing unit for processing the image captured by the imaging unit to obtain a stereofluorescence tomography image; a plurality of excitation light emitting units arranged in a ring on an arc having a first diameter centered on the principal optical axis of the imaging unit, and emitting excitation light beams with a field of view angle toward the subject to excite fluorescence in the subject; a multi-wavelength light source unit connected to the plurality of excitation light emitting units for providing an excitation light source for forming the excitation light beams to the plurality of excitation light emitting units; a ranging unit disposed on the plane of the arc for measuring the distance between the plurality of excitation light emitting units and the subject; and a diameter adjustment unit combined with the plurality of excitation light emitting units to adjust the first diameter according to the distance, so that the field of view of the plurality of excitation light beams forms an overlapping area at the distance that completely covers the subject.

[0012] As one implementation, the diameter adjustment unit can adjust the first diameter according to the spacing distance so that the field of view of the plurality of excitation beams forms the maximum overlap area at the spacing distance.

[0013] As one implementation, a distance adjustment unit may be further included, which is used to adjust the spacing distance so that the diameter adjustment unit adjusts the first diameter according to the adjusted spacing distance, so that the field of view of the plurality of excitation light beams forms an overlapping area at the spacing distance that completely covers the subject being photographed.

[0014] As one implementation, the distance adjustment unit can adjust the spacing distance according to the size of the object being photographed, and the diameter adjustment unit can adjust the first diameter according to the adjusted spacing distance, so that the field of view of the plurality of excitation light beams forms a maximum overlap area at the spacing distance that is consistent with the size of the object being photographed.

[0015] As one implementation, it may further include a plurality of first angle adjustment parts, which are disposed on the diameter adjustment part and combined with the plurality of excitation light emitting parts for adjusting the angle at which the plurality of excitation light emitting parts tilt toward one side of the main optical axis, so as to adjust the overlapping area.

[0016] As one implementation, a wavelength-tunable filter may be further included, which is disposed in front of the field of view of the imaging unit for filtering light incident on the imaging unit.

[0017] As one implementation, it may further include: a tomographic light emitting unit connected to the multi-wavelength light source unit for emitting a tomographic light beam to perform optical coherence tomography on the subject; a first reflector for reflecting the tomographic light beam onto the main optical axis; a beam splitter disposed on the main optical axis for splitting the tomographic light beam reflected by the first reflector into a first beam directed toward the subject along the main optical axis and a transmitted second beam; and a second reflector for reflecting the second beam back to the beam splitter. The multi-wavelength light source unit can provide excitation light sources of different wavelengths to the plurality of excitation light emitting units, so that the plurality of excitation light emitting units emit excitation light beams of different wavelengths to excite fluorescence at different depths of the subject. At the same time, it provides tomographic light sources for forming the tomographic light beam to the tomographic light emitting unit. The wavelength-tunable filter can filter according to the different wavelengths of the excitation light sources and the tomographic light sources, so that the imaging unit can simultaneously obtain fluorescence images of different depths of the subject and optical coherence tomographic images of the entire subject.

[0018] As one implementation, when the subject being photographed contains a self-fluorescent substance or a fluorescent developer, the image processing unit can directly calculate the depth corresponding to each fluorescent image based on the depth information in the optical coherence tomography image, and combine each fluorescent image according to its depth to form a three-dimensional fluorescence tomography image of the subject being photographed.

[0019] As one implementation, when the subject being photographed contains different types of autofluorescent substances and / or fluorescent developers, the image processing unit can extract separate images corresponding to the different types of autofluorescent substances and / or fluorescent developers from each fluorescent image, calculate the depth corresponding to each separate image based on the depth information in the optical coherence tomography image, and combine the separate images corresponding to the different types of autofluorescent substances and / or fluorescent developers according to their depths to form a stereofluorescent tomography image of the subject being photographed corresponding to the different types of autofluorescent substances and / or fluorescent developers.

[0020] As one implementation, a display unit may be further included, which receives and displays the stereofluorescence tomography image in real time.

[0021] As one implementation method, the stereofluorescence layer image can be continuously formed.

[0022] As one implementation, it may further include: a second angle adjustment unit disposed on the diameter adjustment unit and combined with the tomographic light emitting unit for adjusting the angle of the tomographic light emitting unit; and / or a third angle adjustment unit combined with the first reflector for adjusting the angle of the first reflector.

[0023] As one embodiment, the tomographic light emitting section may be disposed on the arc.

[0024] In one embodiment, the diameter adjustment part can be a first adjustment device, which may include: a fixing part, which is in the shape of a ring; a telescopic part, which is formed of a piezoelectric material, is in the shape of a ring, is fixed to the inner circumferential surface of the fixing part, and has a plurality of through holes formed on an arc concentric with the fixing part and having the first diameter; and a power supply part, which is connected to the telescopic part and is used to apply current to the telescopic part to deform the telescopic part and change the first diameter.

[0025] In one implementation, the first adjustment device may be configured to be concentric with the main optical axis.

[0026] In one embodiment, the plurality of excitation light emitting portions may be arranged in each of the through holes in a manner that is tilted toward one side of the main optical axis.

[0027] As one embodiment, the ranging unit may be configured in at least one of the telescopic part and the fixed part.

[0028] As one implementation, the first adjustment device may be further configured with a visible light source, which is used to illuminate the subject with visible light to provide illumination.

[0029] As one implementation, a plurality of second adjustment devices may be further included. The plurality of second adjustment devices may be disposed on the telescopic part and respectively coupled one-to-one with the plurality of light-emitting parts. The plurality of second adjustment devices are formed of piezoelectric material and are connected to the power supply part to adjust the tilt angle of the plurality of light-emitting parts.

[0030] This invention provides a stereofluorescence tomography imaging device, comprising: an imaging unit for capturing images of a subject to obtain fluorescence images and optical coherence tomography images; an image processing unit for processing the images captured by the imaging unit to obtain stereofluorescence tomography images; a plurality of excitation light emitting units for emitting excitation light beams with a field of view angle toward the subject to excite fluorescence in the subject; a tomography light emitting unit for emitting tomography light beams to perform optical coherence tomography on the subject; a first reflecting mirror for reflecting the tomography light beams onto the principal optical axis of the imaging unit; a beam splitter disposed on the principal optical axis for splitting the tomography light beams reflected by the first reflecting mirror into a first beam directed toward the subject along the principal optical axis and a transmitted second beam; a second reflecting mirror for reflecting the second beams back to the beam splitter; and a multi-wavelength light source unit connected to the plurality of excitation light emitting units and the tomography light emitting units for transmitting fluorescence to the subject. The plurality of excitation light emitting units respectively provide excitation light sources of different wavelengths, so that the plurality of excitation light emitting units respectively emit excitation light beams of different wavelengths to excite fluorescence at different depths of the subject being photographed. At the same time, a tomographic light source for forming the tomographic light beam is provided to the tomographic light emitting unit; and a wavelength-tunable filter is disposed in front of the field of view of the imaging unit to filter the light incident on the imaging unit according to the different wavelengths of the excitation light source and the tomographic light source, so that the imaging unit simultaneously obtains fluorescence images of the subject at different depths based on the first fluorescent developer and an optical coherence tomographic image of the subject as a whole. The image processing unit can calculate the depth corresponding to each fluorescence image according to the depth information in the optical coherence tomographic image and combine the fluorescence images according to their depths to form a stereofluorescent tomographic image of the subject corresponding to the first fluorescent developer.

[0031] In one embodiment, the image processing unit can stack the individual fluorescence images according to their depths, and based on each fluorescence image, use interpolation to form a filling image corresponding to the depths between and / or outside the depths corresponding to the individual fluorescence images, so as to form the stereoscopic fluorescence tomographic image of the entire photographed object.

[0032] As one implementation method, the stereofluorescence layer image can be continuously formed.

[0033] In one embodiment, the plurality of excitation light emitting units can be arranged in a ring on an arc having a first diameter centered on the main optical axis of the imaging unit, and emit the excitation light beam toward the object being photographed in a manner tilted toward one side of the main optical axis.

[0034] As one implementation, it may further include: a ranging unit disposed on the plane of the arc for measuring the distance between the plurality of excitation light emitting units and the subject being photographed; and a diameter adjustment unit combined with the plurality of excitation light emitting units to adjust the first diameter according to the distance between them, so that the field of view of the plurality of excitation light beams forms an overlapping area at the distance between them that completely covers the subject being photographed.

[0035] As one implementation, the diameter adjustment unit can adjust the first diameter according to the spacing distance so that the field of view of the plurality of excitation beams forms the maximum overlap area at the spacing distance.

[0036] As one implementation, a distance adjustment unit may be further included, which is used to adjust the spacing distance so that the diameter adjustment unit adjusts the first diameter according to the adjusted spacing distance, so that the field of view of the plurality of excitation light beams forms an overlapping area at the spacing distance that completely covers the subject being photographed.

[0037] As one implementation, the distance adjustment unit can adjust the spacing distance according to the size of the object being photographed, and the diameter adjustment unit can adjust the first diameter according to the adjusted spacing distance, so that the field of view of the plurality of excitation light beams forms a maximum overlap area at the spacing distance that is consistent with the size of the object being photographed.

[0038] As one implementation, it may further include a plurality of first angle adjustment parts, which may be disposed on the diameter adjustment part and combined with the plurality of excitation light emitting parts for adjusting the angle at which the plurality of excitation light emitting parts tilt toward one side of the main optical axis, so as to adjust the overlapping area.

[0039] As one implementation, a display unit may be further included, which receives and displays the stereofluorescence tomography image in real time.

[0040] As one implementation, it may further include: a second angle adjustment unit disposed on the diameter adjustment unit and combined with the tomographic light emitting unit for adjusting the angle of the tomographic light emitting unit; and / or a third angle adjustment unit combined with the first reflector for adjusting the angle of the first reflector.

[0041] As one embodiment, the tomographic light emitting section may be disposed on the arc.

[0042] This invention provides a stereofluorescence tomography imaging device, comprising: an imaging unit for capturing images of a subject to obtain fluorescence images and optical coherence tomography images; an image processing unit for processing the images captured by the imaging unit to obtain stereofluorescence tomography images; a plurality of excitation light emitting units for emitting excitation light beams with a field of view to the subject to excite fluorescence in the subject; a tomography light emitting unit for emitting tomography light beams to perform optical coherence tomography on the subject; a first reflector for reflecting the tomography light beams onto the principal optical axis of the imaging unit; a beam splitter disposed on the principal optical axis for splitting the tomography light beams reflected by the first reflector into a first beam directed toward the subject along the principal optical axis and a transmitted second beam; a second reflector for reflecting the second beam back to the beam splitter; and a multi-wavelength light source unit connected to the plurality of excitation light emitting units and the tomography light emitting units for providing excitation light sources of different wavelengths to the plurality of excitation light emitting units, so that the plurality of excitation light emitting units emit excitation light beams of different wavelengths to excite fluorescence in the subject to excite fluorescence in the subject. Fluorescence is excited at different depths of the subject being photographed. Simultaneously, a tomographic light source is provided to the tomographic light emitting unit to form the tomographic light beam. A wavelength-tunable filter is disposed in front of the field of view of the photographing unit. Based on the different wavelengths of the excitation light source and the tomographic light source, the light incident on the photographing unit is filtered, so that the photographing unit simultaneously obtains fluorescence images of the subject corresponding to different depths of the first and second fluorescent developers, as well as an optical coherence tomographic image of the entire subject. The image processing unit extracts a first separate image corresponding to the first fluorescent developer and a second separate image corresponding to the second fluorescent developer from each fluorescence image. Based on the depth information in the optical coherence tomographic image, it calculates the depths corresponding to each of the first and second separate images, and combines each of the first and second separate images according to their depths to form a stereoscopic fluorescence tomographic image of the subject corresponding to the first fluorescent developer and a stereoscopic fluorescence tomographic image corresponding to the second fluorescent developer.

[0043] In one embodiment, the image processing unit can stack each of the first separated images and the second separated images according to their depths, and based on each of the first separated images and the second separated images, it can form a first filling image corresponding to the depth between and / or other depths corresponding to the depths of each of the first separated images and a second filling image corresponding to the depth between and / or other depths corresponding to the depths of each of the second separated images by interpolation, thereby forming a stereofluorescence tomographic image of the entire photographed object corresponding to the first fluorescent developer and a stereofluorescence tomographic image corresponding to the second fluorescent developer.

[0044] In one embodiment, the image processing unit can associate the first separated images and / or the first filled images of the same depth with each of the first separated images and each of the first filled images.

[0045] As one implementation method, the stereofluorescence layer image can be continuously formed.

[0046] In one embodiment, the plurality of excitation light emitting units can be arranged in a ring on an arc having a first diameter centered on the main optical axis of the imaging unit, and emit the excitation light beam toward the object being photographed in a manner tilted toward one side of the main optical axis.

[0047] As one implementation, it may further include: a ranging unit disposed on the plane of the arc for measuring the distance between the plurality of excitation light emitting units and the subject being photographed; and a diameter adjustment unit combined with the plurality of excitation light emitting units to adjust the first diameter according to the distance between them, so that the field of view of the plurality of excitation light beams forms an overlapping area at the distance between them that completely covers the subject being photographed.

[0048] As one implementation, the diameter adjustment unit can adjust the first diameter according to the spacing distance so that the field of view of the plurality of excitation beams forms the maximum overlap area at the spacing distance.

[0049] As one implementation, a distance adjustment unit may be further included, which is used to adjust the spacing distance so that the diameter adjustment unit adjusts the first diameter according to the adjusted spacing distance, so that the field of view of the plurality of excitation light beams forms an overlapping area at the spacing distance that completely covers the subject being photographed.

[0050] As one implementation, the distance adjustment unit can adjust the spacing distance according to the size of the object being photographed, and the diameter adjustment unit can adjust the first diameter according to the adjusted spacing distance, so that the field of view of the plurality of excitation light beams forms a maximum overlap area at the spacing distance that is consistent with the size of the object being photographed.

[0051] As one implementation, it may further include a plurality of first angle adjustment parts, which are disposed on the diameter adjustment part and combined with the plurality of excitation light emitting parts for adjusting the angle at which the plurality of excitation light emitting parts tilt toward one side of the main optical axis, so as to adjust the overlapping area.

[0052] As one implementation, a display unit may be further included, which receives and displays the stereofluorescence tomography image in real time.

[0053] As one implementation, it may further include: a second angle adjustment unit disposed on the diameter adjustment unit and combined with the tomographic light emitting unit for adjusting the angle of the tomographic light emitting unit; and / or a third angle adjustment unit combined with the first reflector for adjusting the angle of the first reflector.

[0054] As one embodiment, the tomographic light emitting section may be disposed on the arc.

[0055] Beneficial effects

[0056] The stereofluorescence tomography apparatus of the present invention can automatically adjust the field of view of multiple excitation beams according to the distance from the subject being photographed, so that the field of view of multiple excitation beams overlaps on the subject being photographed and completely covers the subject being photographed.

[0057] The stereofluorescence tomography device of the present invention can simultaneously obtain fluorescence images and optical coherence tomography images, and combine the fluorescence images into stereofluorescence tomography images based on the depth information in the optical coherence tomography images.

[0058] The stereofluorescence tomography device of the present invention can simultaneously obtain fluorescence images and optical coherence tomography images, and combine fluorescence images corresponding to different types of autofluorescent substances and / or fluorescent developers into stereofluorescence tomography images based on the depth information in the optical coherence tomography images.

[0059] The beneficial effects that can be achieved by the present invention are not limited to those described above. Those skilled in the art can understand other beneficial effects that the present invention can achieve based on the description of the present invention. Attached Figure Description

[0060] Figure 1 This is a schematic diagram of a stereofluorescence tomography apparatus according to one embodiment of the present invention.

[0061] Figure 2 This is a schematic diagram of a stereofluorescence tomography apparatus according to another embodiment of the present invention.

[0062] Figure 3 This is a bottom view of a plurality of excitation light emitting units according to one embodiment of the present invention.

[0063] Figure 4 This is a schematic diagram of the overlapping configuration of multiple excitation beams according to one embodiment of the present invention.

[0064] Figure 5 yes Figure 2 An enlarged view of part A in the image.

[0065] Figure 6 This is a bottom view of the first adjustment device according to one embodiment of the present invention.

[0066] Figure 7 This is a front sectional view of the first adjusting device according to one embodiment of the present invention.

[0067] Figure 8 This is a schematic diagram of a stereofluorescence tomography apparatus according to another embodiment of the present invention.

[0068] Figure 9 yes Figure 8 An enlarged view of part B in the image.

[0069] Figure 10 This is a bottom view of the light-emitting part according to one embodiment of the present invention.

[0070] Figure 11 This is a schematic diagram of fluorescence imaging according to one embodiment of the present invention.

[0071] Figure Labels

[0072] 10: Stereo-fluorescence tomography device

[0073] 1: Filming Department

[0074] 11: Main optical axis

[0075] 2: Light-emitting part

[0076] 21: Excitation light emitting section

[0077] 211: Excitation beam

[0078] 212: Overlapping region

[0079] 213: Overlapping Space

[0080] 214: Maximum overlap region

[0081] 22: Tomographic light emitting section

[0082] 221: Tomographic beam

[0083] 222: First Beam

[0084] 223: Second Beam

[0085] 224: Irradiation area

[0086] 3: Multi-wavelength light source section

[0087] 4: Wavelength-tunable filter

[0088] 5: Distance measuring unit

[0089] 6: Diameter Adjustment Section

[0090] 61: First regulating device

[0091] 611: Fixing part

[0092] 612: Telescopic section

[0093] 613: Through hole

[0094] 614: Visible light source

[0095] 62: Second regulating device

[0096] 71: First reflecting mirror

[0097] 72: Beam splitter

[0098] 73: Second reflecting mirror

[0099] 81: First Angle Adjustment Section

[0100] 82: Second Angle Adjustment Section

[0101] 83: Third Angle Adjustment Section

[0102] S: Subject of the photograph

[0103] D1: First diameter

[0104] D2: Maintain distance

[0105] d1: Depth 1

[0106] dn: the nth depth

[0107] E1: First excitation beam

[0108] En: The nth excitation beam

[0109] F1: First fluorescence

[0110] Fn: nth fluorescence

[0111] T1: Time 1

[0112] Tn: Time n

[0113] t1: The first time period

[0114] tn: the nth time interval

[0115] λ1: First wavelength

[0116] λn: the nth wavelength Detailed Implementation

[0117] The present invention will now be described in detail through various embodiments, but these are merely examples and the present invention is not limited thereto.

[0118] Furthermore, several terms are used in this invention, defined in a manner that provides a detailed description of various embodiments of the invention. These terms are not limited to their meanings as commonly understood by those skilled in the art, nor are the inventions limited thereto. Specifically, the terms "first," "second," etc., are used only to distinguish different elements and do not constitute a ranking of the elements; the term "wavelength" refers to a band of light with a specific wavelength as its center and a defined range; the term "excitation light" refers to light with a wavelength capable of emitting fluorescence; the term "tomography light" refers to light with a wavelength capable of being used for optical coherence tomography; the term "coherent light" refers to light generated by interference at a beam splitter; and the term "stereochromatographic tomographic image" refers to a stereoscopic image formed by stacking fluorescence images and optical coherence tomographic images at different depths along the depth direction.

[0119] Furthermore, the proportions and dimensions of the various components have been altered or even exaggerated in the accompanying drawings to enable those skilled in the art to understand the various components in this invention. The actual dimensional relationships of the structure of this invention are not limited thereto.

[0120] The stereofluorescence tomography apparatus of the present invention will now be described in detail with reference to the accompanying drawings.

[0121] Figure 1 This is a schematic diagram of a stereofluorescence tomography apparatus according to one embodiment of the present invention.

[0122] Reference Figure 1 The stereofluorescence tomography apparatus 10 of the present invention may include an imaging unit 1, a light-emitting unit 2, a multi-wavelength light source unit 3, and a wavelength-tunable filter 4.

[0123] The imaging unit 1 is used to photograph the subject S to obtain a fluorescence image and an optical coherence tomography (OCT) image of the subject S. The imaging unit 1 may include existing medical imaging cameras (e.g., 3CCD, 3CMOS, 3InGaAs image sensors, etc.), as long as they can obtain fluorescence and OCT images; the present invention is not limited thereto. Furthermore, the subject S may be a patient's body or other biological samples. When the subject S is capable of autofluorescence, it is not necessary to inject a fluorescent contrast agent; otherwise, a fluorescent contrast agent can be pre-injected for fluorescence development. On the other hand, in Figure 1 In this invention, the main optical axis 11 of the imaging unit 1 is oriented vertically downwards, meaning the imaging unit 1 captures images from top to bottom. However, the invention is not limited to this; the imaging unit 1 can be configured to capture images in any direction depending on the actual situation. For example, when it is necessary to capture the front of a human body lying flat on the testing table or the upper surface of a biological sample placed on the experimental table, the imaging unit 1 can be configured as follows: Figure 1As shown, the camera takes pictures from top to bottom; when it is necessary to take pictures of the side of a person lying flat on the testing table or the front, back and side of a person standing, the camera 1 can take pictures in the horizontal direction; when it is necessary to take pictures of the oblique side of a person lying flat on the testing table, the camera 1 can take pictures from the oblique top at an inclined angle.

[0124] The light-emitting unit 2 is used to emit excitation light and tomographic light to the object being photographed S, so as to excite fluorescence in the object being photographed S and perform optical coherence tomography on the object being photographed S, thereby enabling the imaging unit 1 to obtain a fluorescence image and an optical coherence tomographic image. Specifically, the light-emitting unit 2 may include a plurality of excitation light emitting units 21 for emitting excitation light beams 211 and tomographic light emitting units 22 for emitting tomographic light beams 221.

[0125] The multi-wavelength light source unit 3 can be connected to the plurality of light-emitting units 2. Specifically, the multi-wavelength light source unit 3 can be connected to the plurality of excitation light emitting units 21 to provide the plurality of excitation light emitting units 21 with an excitation light source for forming the excitation light beam 211, and can be connected to the tomographic light emitting unit 22 to provide the tomographic light emitting unit 22 with a tomographic light source for forming the tomographic light beam 221.

[0126] The wavelength-tunable filter 4 can be disposed in front of the field of view of the imaging unit 1 to filter the light incident on the imaging unit 1, so that the imaging unit 1 can simultaneously obtain a fluorescence image and an optical coherence tomography image to construct a stereoscopic fluorescence tomography image. For example, the wavelength-tunable filter 4 can adjust the transmitted wavelength as needed to simultaneously transmit fluorescence used to form the fluorescence image and coherent light used to form the optical coherence tomography image, and can filter out background light to improve the signal-to-noise ratio of the fluorescence image and the optical coherence tomography image, thereby improving the imaging effect.

[0127] Although not shown, the stereofluorescence tomography apparatus 10 of the present invention may include an image processing unit. The image processing unit processes the image captured by the imaging unit 1 to obtain a stereofluorescence tomography image. The image processing unit may use image processing devices commonly used in the art, such as computers and processors; the present invention is not limited thereto.

[0128] Figure 2 This is a schematic diagram of a stereofluorescence tomography apparatus according to another embodiment of the present invention. Figure 3 This is a bottom view of a plurality of excitation light emitting units according to one embodiment of the present invention.

[0129] Reference Figure 2 and Figure 3The plurality of excitation light emitting units 21 can be arranged in a ring on an arc with the main optical axis 11 of the imaging unit 1 as the center and having a first diameter D1, so as to avoid blocking the field of view of the imaging unit 1. At the same time, it is ensured that the distance between the plurality of excitation light emitting units 21 and the subject S is the same, so that the illumination effect of each excitation light emitting unit 21 on the subject S is more uniform.

[0130] Furthermore, the plurality of excitation light emitting units 21 can emit excitation light beams 211 towards the object S in a tilted manner toward one side of the main optical axis 11, so that the plurality of excitation light beams 211 converge on the main optical axis 11, thereby forming a point in the field of view of the imaging unit 1 that is jointly illuminated by the plurality of excitation light beams 211. Furthermore, the plurality of excitation light emitting units 21 can emit excitation light beams 211 with a field of view angle (i.e., diffusion angle), so that each excitation light beam 211 has a predetermined field of view, thereby forming an area in the field of view of the imaging unit 1 that is jointly illuminated by the plurality of excitation light beams 211, that is, an overlapping area 212 formed by the overlapping fields of view of the plurality of excitation light beams 211, the overlapping area 212 having a predetermined shape and size. When the object S falls into the overlapping area 212, each of the plurality of excitation light beams 211 can illuminate the object S, therefore, the imaging unit 1 can obtain a fluorescent image composed of the fluorescence excited by one or more of the plurality of excitation light beams 211 on the object S.

[0131] The field of view (FOV) of the excitation beam 211 can be adjusted or set according to actual needs, and the present invention is not limited thereto. As one embodiment, the FAV of the excitation beam 211 can be formed based on the numerical aperture (NA) of the optical fiber. For example, the multi-wavelength light source 3 can be connected to multiple optical fibers and the excitation light source can be provided outward through these multiple optical fibers. In this case, the excitation light emitting unit 21 can be the end of the optical fiber that is not connected to the multi-wavelength light source 3. Based on the numerical aperture, the beam emitted from the end of the optical fiber has a diffusion angle (i.e., FAV). That is, in this way, the multiple excitation light emitting units 21 can emit an excitation beam 211 with a FAV (i.e., diffusion angle). Furthermore, to make the excitation beam 211 more uniform, a diffuser can be further provided on the excitation light emitting unit 21, and other optical devices can also be provided to adjust other optical properties of the excitation beam 211, but the present invention is not limited thereto.

[0132] Figure 4 This is a schematic diagram of the overlapping configuration of multiple excitation beams according to one embodiment of the present invention.

[0133] Reference Figure 4 (a) and Figure 4 In (b), the plurality of excitation beams 211 overlap in space to form an overlapping space 213. Within the overlapping space 213, the field of view of the plurality of excitation beams 211 includes the overlapping region 212. Furthermore, the overlapping space 213 may have an approximately double-conical shape. Therefore, when the object S falls from above into the overlapping space 213, as the distance between the object S and the plurality of excitation beam emitters 21 gradually increases, the size of the overlapping region 212 gradually increases from zero and then gradually decreases back to zero. That is, a maximum overlapping region 214 exists, and the maximum overlapping region 214 is located on the common base of the upper and lower cones of the double cone shape. The distance between the maximum overlapping region 214 and the plurality of excitation beam emitters 21 can be called the "working distance".

[0134] On the other hand, the overlapping space 213 and the maximum overlapping region 214 change according to the first diameter D1. Specifically, in comparison... Figure 4 (a) and Figure 4 In (b), the smaller the first diameter D1, the smaller the distance between the overlapping space 213 and the plurality of excitation light emitting units 21, the smaller the size of the overlapping space 213, the smaller the distance between the maximum overlapping region 214 and the plurality of excitation light emitting units 21 (i.e., the working distance), and the smaller the size of the maximum overlapping region 214; the larger the first diameter D1, the larger the distance between the overlapping space 213 and the plurality of excitation light emitting units 21, the larger the size of the overlapping space 213, the larger the distance between the maximum overlapping region 214 and the plurality of excitation light emitting units 21, and the larger the size of the maximum overlapping region 214.

[0135] That is, there is a direct proportional relationship between the first diameter D1, the distance between the overlapping space 213 and the plurality of excitation light emitting units 21, the size of the overlapping space 213, the distance between the maximum overlapping region 214 and the plurality of excitation light emitting units 21, and the size of the maximum overlapping region 214. This relationship can be calculated by conventional geometric methods in combination with specific parameters such as the angle at which the plurality of excitation light emitting units 21 tilt toward the main optical axis 11 and the field of view angle, which will not be elaborated here.

[0136] The stereofluorescence tomography apparatus 10 of the present invention can precisely adjust the size and position of the overlapping region 212 so that the overlapping region 212 completely covers the object being photographed, thereby enabling the imaging unit 1 to obtain a fluorescence image of the entire surface of the object being photographed S in one go.

[0137] Specifically, refer again Figure 2 The stereofluorescence tomography apparatus 10 of the present invention may further include a ranging unit 5. The ranging unit 5 may be disposed on the plane of the arc and is used to measure the distance D2 between the plurality of excitation light emitting units 21 and the object being photographed S. The ranging unit 5 may use a rangefinder commonly used in the art, for example, a rangefinder based on photoelectric or acoustic waves, but the present invention is not limited thereto.

[0138] Figure 5 yes Figure 2 An enlarged view of part A in the image.

[0139] Reference Figure 5 The stereofluorescence tomography apparatus 10 of the present invention may further include a diameter adjustment unit 6. The diameter adjustment unit 6 is combined with the plurality of excitation light emitting units 21 to adjust the first diameter D1 according to the separation distance D2 measured by the ranging unit 5, so that the field of view of the plurality of excitation light beams 211 forms an overlapping region 212 at the separation distance D2 that completely covers the object S being photographed. Thus, the excitation light beams 211 can completely illuminate the entire surface of the object S at once, thereby simultaneously performing fluorescence imaging on the entire surface of the object S.

[0140] Specifically, when the separation distance D2 measured by the ranging unit 5 is less than the distance between the overlapping space 213 formed under the current first diameter D1 and the plurality of excitation light emitting units 21, the diameter adjustment unit 6 can reduce the first diameter D1 to reduce the distance between the overlapping space 213 and the plurality of excitation light emitting units 21, thereby causing the subject S to fall into the overlapping space 213, so that the overlapping area 212 accurately falls on the subject S.

[0141] When the separation distance D2 measured by the ranging unit 5 is greater than the distance between the overlapping space 213 formed under the current first diameter D1 and the plurality of excitation light emitting units 21, the diameter adjustment unit 6 can increase the first diameter D1 to increase the distance between the overlapping space 213 and the plurality of excitation light emitting units 21, so that the subject S falls into the overlapping space 213, so that the overlapping area 212 falls precisely on the subject S.

[0142] Furthermore, the diameter adjustment unit 6 can adjust the first diameter D1 so that the entire subject S falls into the overlapping space 213, so that the overlapping area 212 completely covers the subject S, thereby forming an overlapping area 212 that completely covers the subject S at the distance D2 between the plurality of excitation light beams 211.

[0143] Preferably, the diameter adjustment unit 6 can adjust the first diameter D1 according to the spacing distance D2 so that the field of view of the plurality of excitation light beams 211 forms the maximum overlap area 214 at the spacing distance D2 (that is, the working distance is adjusted to be the same as the spacing distance D2), thereby facilitating the user to appropriately adjust the spacing distance D2 according to the actual formed maximum overlap area 214 to form a maximum overlap area 214 consistent with the size of the shooting object S, thereby maximizing the utilization rate of the plurality of excitation light beams 211.

[0144] In one embodiment, the diameter adjustment unit 6 can use a drive mechanism commonly used in the art, such as a moving mechanism composed of one or more of a controller, motor, gear, rack, guide wheel, guide rail, etc., but the present invention is not limited thereto. In a preferred embodiment, the diameter adjustment unit 6 can be the first adjustment device described later.

[0145] Figure 6 This is a bottom view of the first adjustment device according to one embodiment of the present invention. Figure 7 This is a front sectional view of the first adjusting device according to one embodiment of the present invention.

[0146] Reference Figure 6 and Figure 7 The first adjusting device 61 may include: a fixing part 611, which is in the shape of an annular disc; a telescopic part 612, which is formed of piezoelectric material, is in the shape of an annular disc, and is fixed to the inner circumferential surface of the fixing part 611, and has a plurality of through holes 613 formed on an arc concentric with it and having the first diameter D1; and a power supply part (not shown), which is connected to the telescopic part 612 and is used to apply current to the telescopic part 612 to deform the telescopic part 612 so as to change the first diameter D1.

[0147] At this time, the first adjustment device 61 can be configured to be concentric with the main optical axis 11, the plurality of excitation light emitting parts 21 can be respectively arranged in each of the through holes 613 in a manner inclined toward one side of the main optical axis 11, and the ranging part 5 can be arranged in at least one of the telescopic part 612 and the fixed part 611 to measure the distance D2 between the plurality of excitation light emitting parts 21 and the shooting object S.

[0148] When it is necessary to photograph the object S, the power supply unit calculates the target diameter based on the measurement result of the ranging unit 5 (i.e., the separation distance D2), and then applies a corresponding current to the telescopic unit 612 according to the target diameter to adjust the first diameter D1 to the target diameter. This causes the fields of view of the plurality of excitation light beams 211 to overlap at the object S and form an overlapping area 212 that completely covers the object S. The target diameter is a value or range of the first diameter D1, which allows the fields of view of the plurality of excitation light beams 211 to form an overlapping area 212 that completely covers the object S at the separation distance D2.

[0149] Additionally, the first adjustment device 61 may be further configured with a visible light source 614, which is used to illuminate the object S with visible light to provide illumination, thereby facilitating user operation. The visible light source 614 may be, for example, as shown below. Figure 6 The annular LED light source shown is configured between the fixed part 611 and the telescopic part 612. However, the present invention is not limited to this. The shape, number, size, power, brightness, etc. of the visible light source 614 can be selected and / or adjusted according to actual needs, as long as it can provide sufficient illumination.

[0150] On the other hand, the stereofluorescence tomography apparatus 10 of the present invention may further include a distance adjustment unit (not shown). The distance adjustment unit is used to adjust the separation distance D2 so that the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms an overlapping area 212 at the separation distance D2 that completely covers the object S being photographed.

[0151] The distance adjustment unit can use a drive mechanism commonly used in the art. For example, it can use a moving mechanism composed of one or more of a controller, motor, gear, rack, guide wheel, guide rail, etc. The present invention is not limited thereto.

[0152] Furthermore, the distance adjustment unit can adjust the spacing distance D2 according to the size of the object being photographed S, and the diameter adjustment unit 6 can adjust the first diameter D1 according to the adjusted spacing distance D2, so that the field of view of the plurality of excitation light beams 211 forms a maximum overlap area 214 at the spacing distance D2 that is consistent with the size of the object being photographed S. The size of the object being photographed S can be manually input by the user or calculated using a visual detection method, and the present invention is not limited thereto.

[0153] As a specific implementation, firstly, the target distance can be calculated based on the size of the object being photographed S. The target distance can be a value of the separation distance D2. When the separation distance D2 is adjusted to the target distance, the fields of view of the plurality of excitation light beams 211 can form a maximum overlap area 214 at the separation distance D2 that is consistent with the size of the object being photographed S. The target distance can be calculated using a separate controller or computer, etc., and the present invention is not limited thereto.

[0154] Subsequently, the distance adjustment unit can adjust the separation distance D2 to the target distance, and the diameter adjustment unit 6 can adjust the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms the maximum overlap area 214 at the separation distance D2 (that is, the working distance is adjusted to be the same as the adjusted separation distance D2).

[0155] Therefore, by coordinating the diameter adjustment unit 6 and the distance adjustment unit, at least one of the first diameter D1 and the separation distance D2 can be appropriately adjusted, thereby maximizing the illumination accuracy of the excitation light beam 211 on the photographed object S and the utilization rate of the excitation light beam 211.

[0156] The field of view (Field of View) angles of the plurality of excitation beams 211 may differ from one another. For example, the plurality of excitation light emitting units 21 may be formed with different Field of View angles, or there may be slight differences in the Field of View angles of the excitation beams 211 emitted by different excitation light emitting units 21 due to unavoidable errors or tolerances in the manufacturing process of each of the plurality of excitation light emitting units 211. For another example, when the excitation light emitting unit 21 is the end of an optical fiber as described above, since different wavelengths of light have different numerical apertures in the same optical fiber, there may be slight differences in the Field of View angles of excitation beams 211 of different wavelengths.

[0157] This difference may affect the overlap rate of the plurality of excitation beams 211, that is, it may affect the sharpness and accuracy of the outline boundary of the overlapping region 212. The requirements for the sharpness and accuracy of the outline boundary of the overlapping region 212 vary depending on the size of the object being photographed S. Specifically, the smaller the size of the object being photographed S, the smaller the size of the overlapping region 212 formed accordingly, and the greater the impact caused by the misalignment of the outline boundary of the overlapping region 212. Therefore, the requirements for the sharpness and accuracy of the outline boundary of the overlapping region 212 are higher.

[0158] The stereofluorescence tomography apparatus 10 of the present invention can precisely adjust the contour boundary of the overlapping region 212 to ensure the clarity and accuracy of the contour boundary of the overlapping region 212.

[0159] Specifically, refer again Figure 5 The stereofluorescence tomography device 10 of the present invention may further include a plurality of first angle adjustment units 81.

[0160] The plurality of first angle adjustment parts 81 may be disposed on the diameter adjustment part 6 and combined with the plurality of excitation light emitting parts 21 for adjusting the angle of inclination of the plurality of excitation light emitting parts 21 toward the main optical axis 11, so as to adjust the overlapping area 212, specifically, to adjust the contour boundary of the overlapping area 212.

[0161] In one embodiment, the plurality of first angle adjustment units 81 may use a rotating mechanism commonly used in the art, for example, a rotating mechanism composed of one or more of a controller, motor, shaft, gear, etc., and the present invention is not limited thereto. In a preferred embodiment, the plurality of first angle adjustment units 81 may be a plurality of second adjustment devices described later.

[0162] Refer again Figure 7 The plurality of second adjustment devices 62 may be formed of piezoelectric material and may be disposed on the telescopic portion 612 of the first adjustment device 61, and respectively coupled one-to-one with the plurality of excitation light emitting portions 21, and connected to the power supply. When it is necessary to adjust the contour boundary of the overlapping region 212, current can be applied to the second adjustment device 62 coupled with the excitation light emitting portion 21 to be adjusted through the power supply, so that the second adjustment device 62 deforms, thereby adjusting the angle at which the excitation light emitting portion 21 tilts towards the main optical axis 11, and finally adjusting the position and angle of overlap between the excitation light beam 211 emitted by the excitation light emitting portion 21 and other excitation light beams 211. Thus, the contour boundary of the overlapping region 212 can be finely adjusted.

[0163] Figure 8 This is a schematic diagram of a stereofluorescence tomography apparatus according to another embodiment of the present invention.

[0164] Reference Figure 8The stereofluorescence tomography apparatus 10 of the present invention may further include: a tomography light emitting unit 22, connected to the multi-wavelength light source unit 3, for emitting a tomography light beam 221 to perform optical coherence tomography on the photographed object S; a first reflecting mirror 71 for reflecting the tomography light beam 221 onto the principal optical axis 11; a beam splitter 72, disposed on the principal optical axis 11, for splitting the tomography light beam 221 reflected by the first reflecting mirror 71 into a first beam 222 directed along the principal optical axis 11 toward the photographed object S and a transmitted second beam 223; and a second reflecting mirror 73 for reflecting the second beam 223 back to the beam splitter 72. Thus, the stereofluorescence tomography apparatus 10 of the present invention can perform optical coherence tomography on the photographed object, thereby obtaining an optical coherence tomography image having depth information of the entire photographed object S.

[0165] Figure 9 yes Figure 8 An enlarged view of part B in the image.

[0166] Reference Figure 9 The stereofluorescence tomography imaging device 10 of the present invention may further include: a second angle adjustment unit 82, disposed on the diameter adjustment unit 6 and combined with the tomographic light emitting unit 22, for adjusting the angle of the tomographic light emitting unit 22; and / or a third angle adjustment unit 83, combined with the first reflector 71, for adjusting the angle of the first reflector 71. Through the second angle adjustment unit 82 and / or the third angle adjustment unit 83, the optical path of the tomographic light beam 221 can be precisely adjusted so that the first beam 222 accurately illuminates the entire surface of the object S being photographed, thereby obtaining the optical coherence tomographic image. The second angle adjustment unit 82 and the third angle adjustment unit 83 may use the same rotating mechanism commonly used in the art as the first angle adjustment unit 81. For example, a rotating mechanism composed of one or more of a controller, motor, shaft, gear, etc., may be used, or it may be formed of a piezoelectric material; the present invention is not limited thereto.

[0167] Figure 10 This is a bottom view of the light-emitting part according to one embodiment of the present invention.

[0168] Reference Figure 10 The tomographic light emitting unit 22 can be disposed on the arc. Furthermore, the tomographic light emitting unit 22 can be arranged together with the plurality of excitation light emitting units 21 in a ring on an arc having a first diameter D1 centered on the main optical axis 11 of the imaging unit 1, so as to avoid obstructing the field of view of the imaging unit 1.

[0169] Furthermore, when the diameter adjustment unit 6 is the first adjustment device 61, the tomographic light emitting unit 22 can be disposed in one of the plurality of through holes 613. In this case, the second angle adjustment unit 82 can use the second adjustment device 62. Therefore, not only can the structure of the stereofluorescence tomography imaging device of the present invention be made more compact to improve space utilization, but the overall number and types of parts constituting the stereofluorescence tomography imaging device of the present invention can also be reduced, thus improving assembly efficiency and maintenance convenience.

[0170] Although not shown, the stereofluorescence tomography imaging apparatus 10 of the present invention may further include a display unit. The display unit can receive and display the stereofluorescence tomography images in real time. The display unit can use display devices commonly used in the art, such as monitors and projectors; the present invention is not limited thereto.

[0171] The hardware structure of the stereofluorescence tomography apparatus 10 of the present invention and the operation of overlapping the fields of view of the plurality of excitation beams 211 have been described in detail above. Hereinafter, the operation of obtaining a fluorescence image by the stereofluorescence tomography apparatus 10 of the present invention and the operation of forming the stereofluorescence tomography image by combining the optical coherence tomography image will be described in detail.

[0172] The stereofluorescence tomography device 10 of the present invention can simultaneously obtain fluorescence images at different depths of the photographed object S and optical coherence tomography images of the photographed object S as a whole. Based on the depth information in the optical coherence tomography images, the depth of each fluorescence image is calculated, and the fluorescence images are combined according to their depths to form the stereofluorescence tomography image.

[0173] Specifically, the multi-wavelength light source unit 3 can provide excitation light sources of different wavelengths to the plurality of excitation light emitting units 21, so that the plurality of excitation light emitting units 21 emit excitation light beams 211 of different wavelengths respectively, thereby exciting fluorescence at different depths of the photographed object S. At the same time, the multi-wavelength light source unit 3 can also provide tomographic light emitting units 22 with tomographic light sources for forming the tomographic light beam 221, so as to perform optical coherence tomography on the photographed object.

[0174] Correspondingly, the wavelength-tunable filter 4 can filter according to the excitation light source and the tomographic light source of different wavelengths, so that the imaging unit 1 can simultaneously obtain fluorescence images of different depths of the subject S and optical coherence tomographic images of the subject S as a whole.

[0175] Figure 11 This is a schematic diagram of fluorescence imaging according to one embodiment of the present invention.

[0176] Reference Figure 11 In (a), the multi-wavelength light source unit 3 can, for example, provide different wavelengths of excitation light sources to n excitation light emitting units 21, that is, from the first excitation light emitting unit to the nth excitation light emitting unit, namely, excitation light sources with wavelengths from the first wavelength λ1 to the nth wavelength λn. Here, n is a natural number greater than 1.

[0177] Specifically, the multi-wavelength light source 3 can sequentially provide the first excitation light emitting unit to the nth excitation light emitting unit with an excitation light source of the first wavelength λ1 to the nth excitation light emitting unit respectively, so that the first excitation light emitting unit to the nth excitation light emitting unit sequentially emits the first excitation light beam E1 of the first wavelength λ1 to the nth excitation light beam En of the nth wavelength λn respectively, thereby sequentially exciting the first fluorescence F1 to the nth fluorescence Fn of the object being photographed S from the first depth d1 to the nth depth dn respectively.

[0178] Correspondingly, the wavelength-tunable filter 4 can transmit the first excitation light source to the nth excitation light source provided by the multi-wavelength light source unit 3, respectively transmitting the first fluorescence F1 to the nth fluorescence Fn, so that the imaging unit 1 sequentially obtains the first fluorescence image of the first depth d1 composed of the first fluorescence F1 to the nth fluorescence image of the nth depth dn composed of the nth fluorescence Fn.

[0179] Preferably, the operation of the multi-wavelength light source 3 providing the excitation light source of different wavelengths can be performed according to a specific time, and the operation of the wavelength-tunable filter 4 filtering the light incident on the imaging unit 1 can also be performed according to the specific time, so that the multi-wavelength light source 3 and the wavelength-tunable filter 4 operate synchronously, thereby enabling the imaging unit 1 to accurately obtain fluorescence images of different depths for the subject S.

[0180] Specifically, refer to Figure 11 In (b), the multi-wavelength light source unit 3 may, for example, provide the first excitation light source with wavelength λ1 to the nth excitation light source with wavelength λn to the first excitation light emitting unit to the nth excitation light emitting unit in the first time period t1 to the nth excitation light emitting unit in the nth time period tn.

[0181] Correspondingly, the wavelength-tunable filter 4 can transmit the first fluorescence F1 to the nth fluorescence Fn during the first time period t1 to the nth time period tn, respectively, from the first time period T1 to the nth time period Tn.

[0182] The time difference between each adjacent time point from the first time point T1 to the nth time point Tn may be the same or different. The (n-1)th time period tn-1 may be less than or equal to the time difference between the (n-1)th time point Tn-1 and the nth time point Tn. The first time period t1 to the nth time period tn may be the same or different from each other. However, each time period from the first time period t1 to the nth time period tn should be within a predetermined time period. The predetermined time period should be appropriately selected based on factors such as computing power and resolution. The total duration from the first time point T1 to the nth time point Tn should be within the predetermined duration. The predetermined duration is sufficient to enable the stereofluorescence tomography imaging device 10 of the present invention to construct and display the stereofluorescence tomography image in real time.

[0183] When the imaging unit 1 obtains the first to the nth fluorescence images of the subject S, the image processing unit can calculate the depth corresponding to each fluorescence image based on the depth information in the simultaneously obtained optical coherence tomography images, stack the fluorescence images according to their depths, and then, based on each fluorescence image, form a filling image corresponding to the depths between and / or outside the depths corresponding to each fluorescence image through interpolation, thereby forming the stereoscopic fluorescence tomography image of the entire subject S. Therefore, the stereoscopic fluorescence tomography imaging device 10 of the present invention can quickly obtain a stereoscopic fluorescence tomography image without scanning the subject S layer by layer.

[0184] The above description assumes that the subject S contains only one type of autofluorescent substance or fluorescent developer. However, in practice, it may be necessary to obtain stereofluorescent tomographic images of different parts of the subject S that require different fluorescent developers. In such cases, it is necessary to inject multiple fluorescent developers into the subject S.

[0185] The following is a detailed description of the operation for obtaining a stereofluorescent tomographic image when the subject S contains multiple autofluorescent substances or fluorescent developers, but content that is repeated above will not be repeated.

[0186] At this time, the multi-wavelength light source unit 3 can provide excitation light sources of different wavelengths to the plurality of excitation light emitting units 21, so that the plurality of excitation light emitting units 21 emit excitation light beams 211 of different wavelengths respectively. At the same time, the multi-wavelength light source unit 3 can also provide tomographic light emitting units 22 with tomographic light sources for forming the tomographic light beam 221, so as to perform optical coherence tomography on the photographed object.

[0187] Correspondingly, the wavelength-tunable filter 4 can filter according to the different wavelengths of the excitation light source and the tomographic light source, so that the imaging unit 1 can simultaneously obtain fluorescence images of the subject S at different depths corresponding to various autofluorescent substances and / or fluorescent developers, as well as an optical coherence tomographic image of the subject S as a whole.

[0188] Specifically, the multi-wavelength light source unit 3 can sequentially provide p types of excitation light sources to p excitation light emitting units 21 for exciting fluorescence of the first fluorescent developer, so as to excite fluorescence based on the first fluorescent developer at p depths of the photographed object S, thereby obtaining p fluorescent images corresponding to the first fluorescent developer. The multi-wavelength light source unit 3 can also sequentially provide q types of excitation light sources to q excitation light emitting units 21 for exciting fluorescence of the second fluorescent developer, so as to excite fluorescence based on the second fluorescent developer at q depths of the photographed object S, thereby obtaining q fluorescent images corresponding to the second fluorescent developer. There may be overlap between the p types of excitation light sources for exciting fluorescence of the first fluorescent developer and the q types of excitation light sources for exciting fluorescence of the second fluorescent developer; that is, there may be the same excitation light emitting unit 21 between the p excitation light emitting units 21 and the q excitation light emitting units 21. This is determined by the excitation wavelength of the first fluorescent developer and the second fluorescent developer themselves, and the present invention is not limited thereto.

[0189] When the imaging unit 1 obtains a fluorescence image of the subject S corresponding to the first fluorescent developer and a fluorescence image corresponding to the second fluorescent developer, the image processing unit can extract a first separated image corresponding to the first fluorescent developer and a second separated image corresponding to the second fluorescent developer from each fluorescence image. Based on the depth information in the optical coherence tomography image obtained simultaneously, the unit calculates the depth corresponding to each first separated image and the second separated image, and combines each first separated image and the second separated image according to their depths to form a stereofluorescence tomography image of the subject S corresponding to the first fluorescent developer and a stereofluorescence tomography image corresponding to the second fluorescent developer.

[0190] Specifically, the image processing unit can stack each of the first separated images and the second separated images according to their depths, and based on each of the first separated images and the second separated images, it uses interpolation to form a first filling image corresponding to the depths between and / or outside the depths corresponding to each of the first separated images, and a second filling image corresponding to the depths between and / or outside the depths corresponding to each of the second separated images, thereby forming a stereofluorescence tomographic image of the entire photographed object S corresponding to the first fluorescent developer and a stereofluorescence tomographic image corresponding to the second fluorescent developer. Therefore, the stereofluorescence tomographic imaging device 10 of the present invention can quickly obtain stereofluorescence tomographic images corresponding to different fluorescent developers without scanning the photographed object S layer by layer.

[0191] Furthermore, the image processing unit can combine the stereofluorescence tomographic image of the photographed object S corresponding to the first fluorescent developer with the stereofluorescence tomographic image corresponding to the second fluorescent developer. That is, the image processing unit can correlate the first separated images and / or the first filled images of the same depth of each of the first separated images and each of the first filled images to form the stereofluorescence tomographic image of the entire photographed object S. Thus, the stereofluorescence tomographic imaging apparatus 10 of the present invention can quickly obtain stereofluorescence tomographic images of different fluorescent developers located at different locations simultaneously without scanning the photographed object S layer by layer.

[0192] The above provides a detailed description of the operation of the stereofluorescence tomography apparatus 10 of the present invention in forming the stereofluorescence tomography image. The stereofluorescence tomography apparatus 10 can continuously form the stereofluorescence tomography image. Therefore, the stereofluorescence tomography apparatus 10 of the present invention can not only assist doctors in observing a wide range of areas at once, but also allow doctors to observe the current condition of the surgical site (i.e., the object being photographed S) in real time during surgery.

[0193] The stereofluorescence tomography imaging device of the present invention has been described in detail above. However, these are merely multiple embodiments of the present invention, and the present invention is not limited thereto. Those skilled in the art can make various modifications within the scope of the technical analysis of the present invention, and all such modifications fall within the protection scope of the claims of the present invention.

[0194] The present invention can also be implemented in the following ways.

[0195] Project 1-1. A stereofluorescence tomography imaging device 10, characterized in that it comprises: The imaging unit 1 is used to photograph the subject S to obtain fluorescence images and optical coherence tomography images; The image processing unit is used to process the images captured by the imaging unit 1 to obtain stereofluorescence tomography images; Multiple excitation light emitting units 21 are arranged in a ring on an arc having a first diameter D1 centered on the main optical axis 11 of the imaging unit 1, and emit excitation light beams 211 with a field of view angle toward the subject S in a manner tilted toward one side of the main optical axis 11 to excite fluorescence in the subject S. The multi-wavelength light source unit 3 is connected to the plurality of excitation light emitting units 21 and is used to provide the plurality of excitation light emitting units 21 with an excitation light source for forming the excitation light beam 211; The ranging unit 5, disposed on the plane of the arc, is used to measure the distance D2 between the plurality of excitation light emitting units 21 and the object being photographed S; and The diameter adjustment unit 6 is combined with the plurality of excitation light emitting units 21 to adjust the first diameter D1 according to the separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms an overlapping area 212 at the separation distance D2 that completely covers the subject S.

[0196] Project 1-2. The stereofluorescence tomography imaging device 10 according to Project 1-1 is characterized in that, The diameter adjustment unit 6 adjusts the first diameter D1 according to the separation distance D2 so that the field of view of the plurality of excitation light beams 211 forms a maximum overlap area 214 at the separation distance D2.

[0197] Item 1-3. The stereofluorescence tomography imaging device 10 according to Item 1-1 is characterized in that, It further includes a distance adjustment unit, The distance adjustment unit is used to adjust the separation distance D2 so that the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms an overlapping area 212 at the separation distance D2 that completely covers the subject S.

[0198] Items 1-4. The stereofluorescence tomography imaging device 10 according to Items 1-3 is characterized in that, The distance adjustment unit adjusts the separation distance D2 according to the size of the object being photographed S, and the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms a maximum overlap area 214 at the separation distance D2 that is consistent with the size of the object being photographed S.

[0199] Items 1-5. The stereofluorescence tomography imaging device 10 according to Item 1-1 is characterized in that, It further includes multiple first angle adjustment units 81, The plurality of first angle adjustment parts 81 are disposed on the diameter adjustment part 6 and are combined with the plurality of excitation light emitting parts 21 to adjust the angle at which the plurality of excitation light emitting parts 21 tilt toward the main optical axis 11, so as to adjust the overlapping area 212.

[0200] Items 1-6. The stereofluorescence tomography imaging device 10 according to Item 1-1 is characterized in that, Further includes a wavelength-tunable filter 4, The wavelength-tunable filter 4 is positioned in front of the field of view of the imaging unit 1 and is used to filter the light incident on the imaging unit 1.

[0201] Items 1-7. The stereofluorescence tomography imaging apparatus 10 according to Items 1-6, characterized in that it further comprises: The tomographic light emitting unit 22 is connected to the multi-wavelength light source unit 3 and is used to emit a tomographic light beam 221 to perform optical coherence tomography on the photographed object S. The first reflecting mirror 71 is used to reflect the tomographic beam 221 onto the main optical axis 11; Beam splitter 72, disposed on the main optical axis 11, is used to split the tomographic beam 221 reflected by the first reflector 71 into a first beam 222 directed along the main optical axis 11 toward the object S being photographed and a second beam 223 transmitted therethrough; and The second reflector 73 reflects the second beam 223 back to the beam splitter 72. The multi-wavelength light source unit 3 provides excitation light sources of different wavelengths to the plurality of excitation light emitting units 21, so that the plurality of excitation light emitting units 21 emit excitation light beams 211 of different wavelengths to excite fluorescence at different depths of the photographed object S. At the same time, it provides tomographic light emitting units 22 with tomographic light sources for forming the tomographic light beams 221. The wavelength-tunable filter 4 filters according to the excitation light source and the tomographic light source of different wavelengths, so that the imaging unit 1 can simultaneously obtain fluorescence images of different depths of the subject S and optical coherence tomographic images of the subject S as a whole.

[0202] Items 1-8. The stereofluorescence tomography imaging device 10 according to Items 1-7 is characterized in that, When the object being photographed S contains a self-fluorescent substance or a fluorescent developer, the image processing unit directly calculates the depth corresponding to each fluorescent image based on the depth information in the optical coherence tomography image, and combines each fluorescent image according to its depth to form a stereofluorescence tomography image of the object being photographed S.

[0203] Items 1-9. The stereofluorescence tomography imaging device 10 according to Items 1-7 is characterized in that, When the object being photographed S contains different types of autofluorescent substances and / or fluorescent developers, the image processing unit extracts separate images corresponding to the different types of autofluorescent substances and / or fluorescent developers from each fluorescent image, calculates the depth corresponding to each separate image based on the depth information in the optical coherence tomography image, and combines the separate images corresponding to the different types of autofluorescent substances and / or fluorescent developers according to their depths to form a stereofluorescent tomography image of the object being photographed S corresponding to the different types of autofluorescent substances and / or fluorescent developers.

[0204] Items 1-10. The stereofluorescence tomography imaging apparatus 10 according to any one of Items 1-8 and 1-9, characterized in that, It further includes a display unit that receives and displays the stereofluorescence tomography image in real time.

[0205] Items 1-11. The stereofluorescence tomography imaging apparatus 10 according to any one of Items 1-8 and 1-9, characterized in that, The stereofluorescence layer image is continuously formed.

[0206] Items 1-12. The stereofluorescence tomography imaging apparatus 10 according to Items 1-7, characterized in that it further comprises: A second angle adjustment unit 82 is disposed on the diameter adjustment unit 6 and is combined with the tomographic light emitting unit 22 for adjusting the angle of the tomographic light emitting unit 22; and / or The third angle adjustment unit 83 is combined with the first reflector 71 and is used to adjust the angle of the first reflector 71.

[0207] Items 1-13. The stereofluorescence tomography imaging device 10 according to Items 1-7 is characterized in that, The tomographic light emitting part 22 is disposed on the arc.

[0208] Item 1-14. The stereofluorescence tomography imaging device 10 according to Item 1-1 is characterized in that, The diameter adjustment section 6 is the first adjustment device 61. The first adjusting device 61 includes: The fixing part 611 is in the shape of a ring disc; The telescopic part 612, formed of piezoelectric material, is annular in shape and fixed to the inner circumferential surface of the fixing part 611. It has multiple through holes 613 formed on an arc concentric with the fixing part 611 and having the first diameter D1. The power supply unit is connected to the telescopic part 612 and is used to apply current to the telescopic part 612 so that the telescopic part 612 deforms to change the first diameter D1.

[0209] Items 1-15. The stereofluorescence tomography imaging device 10 according to Items 1-14 is characterized in that, The first adjustment device 61 is configured to be concentric with the main optical axis 11.

[0210] Items 1-16. The stereofluorescence tomography imaging device 10 according to Items 1-14 is characterized in that, The plurality of excitation light emitting units 21 are respectively arranged in each of the through holes 613 in a manner that is inclined toward one side of the main optical axis 11.

[0211] Items 1-17. The stereofluorescence tomography imaging device 10 according to Items 1-14 is characterized in that, The ranging part 5 is disposed in at least one of the telescopic part 612 and the fixed part 611.

[0212] Items 1-18. The stereofluorescence tomography imaging device 10 according to Items 1-14 is characterized in that, The first adjustment device 61 is further configured with a visible light source 614, which is used to irradiate visible light toward the photographed object S to provide illumination.

[0213] Items 1-19. The stereofluorescence tomography imaging device 10 according to Items 1-14 is characterized in that, It further includes multiple second adjustment devices 62, The plurality of second adjustment devices 62 are disposed on the telescopic part 612 and are respectively coupled to the plurality of light-emitting parts 2 one-to-one. The plurality of second adjustment devices 62 are formed of piezoelectric material and are connected to the power supply unit to adjust the tilt angle of the plurality of light-emitting units 2.

[0214] Project 2-1. A stereofluorescence tomography imaging device 10, characterized in that it comprises: The imaging unit 1 is used to photograph the subject S to obtain fluorescence images and optical coherence tomography images; The image processing unit is used to process the images captured by the imaging unit 1 to obtain stereofluorescence tomography images; Multiple excitation light emitting units 21 are used to emit excitation light beams 211 with a field of view to the photographed object S, so as to excite fluorescence in the photographed object S; The tomographic light emitting unit 22 is used to emit a tomographic light beam 221 to perform optical coherence tomography on the photographed object S; The first reflecting mirror 71 is used to reflect the tomographic light beam 221 onto the main optical axis 11 of the imaging unit 1; Beam splitter 72, disposed on the main optical axis 11, is used to split the tomographic light beam 221 reflected by the first reflector 71 into a first beam 222 directed along the main optical axis 11 toward the photographed object S and a transmitted second beam 223. The second reflector 73 reflects the second beam 223 back to the beam splitter 72; A multi-wavelength light source unit 3, connected to the plurality of excitation light emitting units 21 and the tomographic light emitting unit 22, is used to provide excitation light sources of different wavelengths to the plurality of excitation light emitting units 21, so that the plurality of excitation light emitting units 21 emit excitation light beams 211 of different wavelengths to excite fluorescence at different depths of the photographed object S. Simultaneously, it provides tomographic light sources to the tomographic light emitting unit 22 for forming the tomographic light beam 221; and A wavelength-tunable filter 4 is disposed in front of the field of view of the imaging unit 1. Based on the excitation light source and the tomographic light source of different wavelengths, it filters the light incident on the imaging unit 1, so that the imaging unit 1 simultaneously obtains fluorescence images of the subject S at different depths based on the first fluorescent developer, as well as an optical coherence tomographic image of the subject S as a whole. The image processing unit calculates the depth corresponding to each fluorescence image based on the depth information in the optical coherence tomography image, and combines each fluorescence image according to its depth to form a stereofluorescence tomography image of the photographed object S corresponding to the first fluorescent developer.

[0215] Project 2-2. The stereofluorescence tomography imaging device 10 according to Project 2-1 is characterized in that, The image processing unit stacks the fluorescent images according to their depths, and based on each fluorescent image, it uses interpolation to form a filling image corresponding to the depths between and / or outside the depths corresponding to each fluorescent image, so as to form the stereoscopic fluorescence tomographic image of the entire photographed object S.

[0216] Project 2-3. The stereofluorescence tomography imaging device 10 according to Project 2-1 is characterized in that, The stereofluorescence layer image is continuously formed.

[0217] Project 2-4. The stereofluorescence tomography imaging device 10 according to Project 2-1 is characterized in that, The plurality of excitation light emitting units 21 are arranged in a ring on an arc having a first diameter D1 centered on the main optical axis 11 of the imaging unit 1, and emit the excitation light beam 211 toward the imaging object S in a manner tilted toward one side of the main optical axis 11.

[0218] Item 2-5. The stereofluorescence tomography imaging device 10 according to Item 2-1, characterized in that it further comprises: The ranging unit 5, disposed on the plane of the arc, is used to measure the distance D2 between the plurality of excitation light emitting units 21 and the object being photographed S; and The diameter adjustment unit 6 is combined with the plurality of excitation light emitting units 21 to adjust the first diameter D1 according to the separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms an overlapping area 212 at the separation distance D2 that completely covers the subject S.

[0219] Item 2-6. The stereofluorescence tomography imaging device 10 according to Item 2-5 is characterized in that, The diameter adjustment unit 6 adjusts the first diameter D1 according to the separation distance D2 so that the field of view of the plurality of excitation light beams 211 forms a maximum overlap area 214 at the separation distance D2.

[0220] Item 2-7. The stereofluorescence tomography imaging device 10 according to Item 2-5 is characterized in that, It further includes a distance adjustment unit, The distance adjustment unit is used to adjust the separation distance D2 so that the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms an overlapping area 212 at the separation distance D2 that completely covers the subject S.

[0221] Item 2-8. The stereofluorescence tomography imaging device 10 according to Item 2-7 is characterized in that, The distance adjustment unit adjusts the separation distance D2 according to the size of the object being photographed S, and the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms a maximum overlap area 214 at the separation distance D2 that is consistent with the size of the object being photographed S.

[0222] Item 2-9. The stereofluorescence tomography imaging device 10 according to Item 2-5 is characterized in that, It further includes multiple first angle adjustment units 81, The plurality of first angle adjustment parts 81 are disposed on the diameter adjustment part 6 and are combined with the plurality of excitation light emitting parts 21 to adjust the angle at which the plurality of excitation light emitting parts 21 tilt toward the main optical axis 11, so as to adjust the overlapping area 212.

[0223] Item 2-10. The stereofluorescence tomography imaging device 10 according to Item 2-1 is characterized in that, It further includes a display unit that receives and displays the stereofluorescence tomography image in real time.

[0224] Item 2-11. The stereofluorescence tomography imaging device 10 according to Item 2-5, characterized in that it further comprises: A second angle adjustment unit 82 is disposed on the diameter adjustment unit 6 and is combined with the tomographic light emitting unit 22 for adjusting the angle of the tomographic light emitting unit 22; and / or The third angle adjustment unit 83 is combined with the first reflector 71 and is used to adjust the angle of the first reflector 71.

[0225] Item 2-12. The stereofluorescence tomography imaging device 10 according to Item 2-11 is characterized in that, The tomographic light emitting part 22 is disposed on the arc.

[0226] Project 3-1. A stereofluorescence tomography imaging device 10, characterized in that it comprises: The imaging unit 1 is used to photograph the subject S to obtain fluorescence images and optical coherence tomography images; The image processing unit is used to process the images captured by the imaging unit 1 to obtain stereofluorescence tomography images; Multiple excitation light emitting units 21 are used to emit excitation light beams 211 with a field of view to the photographed object S, so as to excite fluorescence in the photographed object S; The tomographic light emitting unit 22 is used to emit a tomographic light beam 221 to perform optical coherence tomography on the photographed object S; The first reflecting mirror 71 is used to reflect the tomographic light beam 221 onto the main optical axis 11 of the imaging unit 1; Beam splitter 72, disposed on the main optical axis 11, is used to split the tomographic light beam 221 reflected by the first reflector 71 into a first beam 222 directed along the main optical axis 11 toward the photographed object S and a transmitted second beam 223. The second reflector 73 reflects the second beam 223 back to the beam splitter 72; A multi-wavelength light source unit 3, connected to the plurality of excitation light emitting units 21 and the tomographic light emitting unit 22, is used to provide excitation light sources of different wavelengths to the plurality of excitation light emitting units 21, so that the plurality of excitation light emitting units 21 emit excitation light beams 211 of different wavelengths to excite fluorescence at different depths of the photographed object S. Simultaneously, it provides tomographic light sources to the tomographic light emitting unit 22 for forming the tomographic light beam 221; and A wavelength-tunable filter 4 is disposed in front of the field of view of the imaging unit 1. Based on the excitation light source and the tomographic light source of different wavelengths, it filters the light incident on the imaging unit 1, so that the imaging unit 1 simultaneously obtains a fluorescence image of the object S corresponding to different depths of the first and second fluorescent developers, as well as an optical coherence tomographic image of the object S as a whole. The image processing unit extracts a first separation image corresponding to the first fluorescent developer and a second separation image corresponding to the second fluorescent developer from each fluorescent image. It calculates the depth corresponding to each first separation image and the second separation image based on the depth information in the optical coherence tomography image, and combines each first separation image and the second separation image according to their depth to form a stereofluorescence tomography image of the photographed object S corresponding to the first fluorescent developer and a stereofluorescence tomography image corresponding to the second fluorescent developer.

[0227] Project 3-2. The stereofluorescence tomography imaging device 10 according to Project 3-1 is characterized in that, The image processing unit stacks each of the first separated images and the second separated images according to their depths, and based on each of the first separated images and the second separated images, it uses interpolation to form a first filling image corresponding to the depth between and / or other depths of each of the first separated images and a second filling image corresponding to the depth between and / or other depths of each of the second separated images, thereby forming a stereofluorescence tomographic image of the entire photographed object S corresponding to the first fluorescent developer and a stereofluorescence tomographic image corresponding to the second fluorescent developer.

[0228] Project 3-3. The stereofluorescence tomography imaging device 10 according to Project 3-2 is characterized in that, The image processing unit associates the first separated images and / or the first filled images of the same depth with each of the first separated images and each of the first filled images.

[0229] Project 3-4. The stereofluorescence tomography imaging device 10 according to Project 3-1 is characterized in that, The stereofluorescence layer image is continuously formed.

[0230] Project 3-5. The stereofluorescence tomography imaging device 10 according to Project 3-1 is characterized in that, The plurality of excitation light emitting units 21 are arranged in a ring on an arc having a first diameter D1 centered on the main optical axis 11 of the imaging unit 1, and emit the excitation light beam 211 toward the imaging object S in a manner tilted toward one side of the main optical axis 11.

[0231] Item 3-6. The stereofluorescence tomography imaging device 10 according to Item 3-1, characterized in that it further comprises: The ranging unit 5, disposed on the plane of the arc, is used to measure the distance D2 between the plurality of excitation light emitting units 21 and the object being photographed S; and The diameter adjustment unit 6 is combined with the plurality of excitation light emitting units 21 to adjust the first diameter D1 according to the separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms an overlapping area 212 at the separation distance D2 that completely covers the subject S.

[0232] Item 3-7. The stereofluorescence tomography imaging device 10 according to Item 3-6 is characterized in that, The diameter adjustment unit 6 adjusts the first diameter D1 according to the separation distance D2 so that the field of view of the plurality of excitation light beams 211 forms a maximum overlap area 214 at the separation distance D2.

[0233] Item 3-8. The stereofluorescence tomography imaging device 10 according to Item 3-6 is characterized in that, It further includes a distance adjustment unit, The distance adjustment unit is used to adjust the separation distance D2 so that the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms an overlapping area 212 at the separation distance D2 that completely covers the subject S.

[0234] Item 3-9. The stereofluorescence tomography imaging device 10 according to Item 3-8 is characterized in that, The distance adjustment unit adjusts the separation distance D2 according to the size of the object being photographed S, and the diameter adjustment unit 6 adjusts the first diameter D1 according to the adjusted separation distance D2, so that the field of view of the plurality of excitation light beams 211 forms a maximum overlap area 214 at the separation distance D2 that is consistent with the size of the object being photographed S.

[0235] Item 3-10. The stereofluorescence tomography imaging device 10 according to Item 3-6 is characterized in that, It further includes multiple first angle adjustment units 81, The plurality of first angle adjustment parts 81 are disposed on the diameter adjustment part 6 and are combined with the plurality of excitation light emitting parts 21 to adjust the angle at which the plurality of excitation light emitting parts 21 tilt toward the main optical axis 11, so as to adjust the overlapping area 212.

[0236] Item 3-11. The stereofluorescence tomography imaging device 10 according to Item 3-1 is characterized in that, It further includes a display unit that receives and displays the stereofluorescence tomography image in real time.

[0237] Item 3-12. The stereofluorescence tomography imaging device 10 according to Item 3-6, characterized in that it further comprises: A second angle adjustment unit 82 is disposed on the diameter adjustment unit 6 and is combined with the tomographic light emitting unit 22 for adjusting the angle of the tomographic light emitting unit 22; and / or The third angle adjustment unit 83 is combined with the first reflector 71 and is used to adjust the angle of the first reflector 71.

[0238] Item 3-13. The stereofluorescence tomography imaging device 10 according to Item 3-12 is characterized in that, The tomographic light emitting part 22 is disposed on the arc.

Claims

1. A stereofluorescence tomography imaging device (10), characterized in that, include: The imaging unit (1) is used to photograph the subject (S) to obtain fluorescence images and optical coherence tomography images; The image processing unit is used to process the images captured by the imaging unit (1) to obtain stereofluorescence tomography images; Multiple excitation light emitting units (21) are used to emit excitation light beams (211) with a field of view to the photographed object (S) to excite fluorescence in the photographed object (S); The tomographic light emitting unit (22) is used to emit a tomographic light beam (221) to perform optical coherence tomography on the photographed object (S); The first reflecting mirror (71) is used to reflect the tomographic light beam (221) onto the main optical axis (11) of the imaging unit (1); A beam splitter (72) is disposed on the main optical axis (11) to split the tomographic beam (221) reflected by the first reflector (71) into a first beam (222) directed toward the photographed object (S) along the main optical axis (11) and a second beam (223) transmitted. The second reflector (73) reflects the second beam (223) back to the beam splitter (72); The multi-wavelength light source unit (3) is connected to the plurality of excitation light emitting units (21) and the tomographic light emitting unit (22), and is used to provide excitation light sources of different wavelengths to the plurality of excitation light emitting units (21) respectively, so that the plurality of excitation light emitting units (21) sequentially emit excitation light beams (211) of different wavelengths within a predetermined time period, so as to sequentially excite fluorescence at multiple different depths of the photographed object (S), and at the same time, provides the tomographic light emitting unit (22) with a tomographic light source for forming the tomographic light beam (221); as well as A wavelength-tunable filter (4) is disposed in front of the field of view of the imaging unit (1). Based on the excitation light source and the tomographic light source of different wavelengths, the filter targets the imaging unit (1) so that the imaging unit (1) simultaneously obtains a fluorescence image of the subject (S) corresponding to different depths of the first and second fluorescent developers, as well as an optical coherence tomographic image of the subject (S) as a whole. The image processing unit extracts a first separation image corresponding to the first fluorescent developer and a second separation image corresponding to the second fluorescent developer from each fluorescent image. It calculates the depth corresponding to each first separation image and the second separation image based on the depth information in the optical coherence tomography image, and combines each first separation image and the second separation image according to their depth to form a stereofluorescence tomography image of the photographed object (S) corresponding to the first fluorescent developer and a stereofluorescence tomography image corresponding to the second fluorescent developer.

2. The stereofluorescence tomography imaging device (10) according to claim 1, characterized in that, The image processing unit stacks each of the first separated images and the second separated images according to their depths, and based on each of the first separated images and the second separated images, it uses interpolation to form a first filling image corresponding to the depth between and / or other depths of each of the first separated images and a second filling image corresponding to the depth between and / or other depths of each of the second separated images, thereby forming a stereofluorescence tomographic image of the entire photographed object (S) corresponding to the first fluorescent developer and a stereofluorescence tomographic image corresponding to the second fluorescent developer.

3. The stereofluorescence tomography imaging device (10) according to claim 2, characterized in that, The image processing unit associates the first separated images and / or the first filled images of the same depth with each of the first separated images and each of the first filled images.

4. The stereofluorescence tomography imaging device (10) according to claim 1, characterized in that, The stereofluorescence layer image is continuously formed.

5. The stereofluorescence tomography imaging device (10) according to claim 1, characterized in that, The plurality of excitation light emitting units (21) are arranged in a ring on an arc having a first diameter (D1) centered on the main optical axis (11) of the shooting unit (1), and emit the excitation light beam (211) toward the shooting object (S) in a manner tilted toward one side of the main optical axis (11).

6. The stereofluorescence tomography imaging device (10) according to claim 1, characterized in that, Further includes: The ranging unit (5), disposed on the plane of the arc, is used to measure the distance (D2) between the plurality of excitation light emitting units (21) and the object being photographed (S); and The diameter adjustment unit (6) is combined with the plurality of excitation light emitting units (21) to adjust the first diameter (D1) according to the separation distance (D2) so that the field of view of the plurality of excitation light beams (211) forms an overlapping area (212) that completely covers the photographed object (S) at the separation distance (D2).

7. The stereofluorescence tomography imaging device (10) according to claim 6, characterized in that, The diameter adjustment unit (6) adjusts the first diameter (D1) according to the separation distance (D2) so that the field of view of the plurality of excitation light beams (211) forms the maximum overlap area (214) at the separation distance (D2).

8. The stereofluorescence tomography imaging device (10) according to claim 6, characterized in that, It further includes a distance adjustment unit, The distance adjustment unit is used to adjust the separation distance (D2) so that the diameter adjustment unit (6) adjusts the first diameter (D1) according to the adjusted separation distance (D2) so that the field of view of the plurality of excitation light beams (211) forms an overlapping area (212) that covers the entire subject (S) at the separation distance (D2).

9. The stereofluorescence tomography imaging device (10) according to claim 8, characterized in that, The distance adjustment unit adjusts the separation distance (D2) according to the size of the subject (S), and the diameter adjustment unit (6) adjusts the first diameter (D1) according to the adjusted separation distance (D2) so that the field of view of the plurality of excitation light beams (211) forms a maximum overlap area (214) at the separation distance (D2) that is consistent with the size of the subject (S).

10. The stereofluorescence tomography imaging device (10) according to claim 6, characterized in that, It further includes multiple first angle adjustment units (81). The plurality of first angle adjustment parts (81) are disposed on the diameter adjustment part (6) and combined with the plurality of excitation light emitting parts (21) to adjust the angle of the plurality of excitation light emitting parts (21) tilting toward the main optical axis (11) to adjust the overlapping area (212).

11. The stereofluorescence tomography imaging device (10) according to claim 1, characterized in that, It further includes a display unit that receives and displays the stereofluorescence tomography image in real time.

12. The stereofluorescence tomography imaging device (10) according to claim 6, characterized in that, Further includes: A second angle adjustment section (82) is disposed on the diameter adjustment section (6) and combined with the tomographic light emitting section (22) for adjusting the angle of the tomographic light emitting section (22); and / or The third angle adjustment unit (83) is combined with the first reflector (71) to adjust the angle of the first reflector (71).

13. The stereofluorescence tomography imaging device (10) according to claim 12, characterized in that, The tomographic light emitting part (22) is disposed on the arc.

Images