Application of tussilagone in preparation of anti-radiation injury medicine

Abstract

The invention discloses application of tussilagone in preparation of a medicine for resisting radiation injury, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to resist radiation damage. The application provided by the invention specifically refers to application of tussilagone in preparation of a product for treating or / and relieving body injury caused by radiation. Experiments prove that tussilagone can effectively relieve hyperemia and bleeding and inflammatory cell infiltration of pulmonary alveoli of mice after radiation; the hepatic cord structure damage and hepatic sinus hyperemia in the hepatic tissue are relieved; disorder and vacuolation of a bone marrow structure can be relieved, and recovery of hematopoietic cells in bone marrow is promoted; edema and shedding of intestinal villi can be relieved, and the integrity of the intestinal structure can be partially repaired; and hair follicle structure damage and fiber bundle hyperplasia in skin tissues are improved.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, specifically relating to the application of coltsfoot ketone in the preparation of drugs for preventing radiation damage. Background Technology

[0002] Ionizing radiation injuries are commonly seen in nuclear attacks, nuclear power plant accidents, nuclear terrorism, irradiation device accidents, and radioactive source accidents. They are characterized by severe injuries, rapid progression, difficulty in treatment, and high mortality rates. Radiation is also used in tumor radiotherapy and has a wide range of applications in civilian settings. Ionizing radiation typically causes simultaneous damage to multiple tissues and organs, including the hematopoietic system, gastrointestinal tract, and skin. In addition to focusing on damage to specific sensitive tissues and organs, there is an urgent need to explore and establish treatment techniques and drugs for ionizing radiation injuries affecting multiple tissues and organs.

[0003] Furthermore, the difficulty of treating damage to different tissues and organs caused by ionizing radiation varies. Currently approved drugs for treating ionizing radiation injury in China are mainly estrogen-based, with significant estrogenic side effects. FDA-approved drugs for treating ionizing radiation injury are mainly cytokines, and are only used for the protection or treatment of myelopathic acute radiation sickness. In addition, radioprotective agents, whether already approved or currently undergoing clinical trials, all have shortcomings requiring improvement, including being used only for post-radiation relief, requiring injection, having a narrow protective window, high production costs, being difficult to transport and store, and having significant toxic side effects. Currently, no drugs are approved for the prevention and treatment of multi-organ damage caused by ionizing radiation. In recent years, plant compounds have shown good effects in the prevention and treatment of many diseases. Researchers have discovered that some natural small molecules have protective effects against radiation damage to the hematopoietic system and intestines of rodents. Traditional Chinese medicine and its theories are unique and valuable resources in my country. With its advantages of wide availability and low toxicity, the use of traditional Chinese medicine to prevent and treat damage caused by ionizing radiation has begun to attract widespread attention. Summary of the Invention

[0004] The technical problem to be solved by this invention is how to resist radiation damage. The technical problem to be solved is not limited to the described technical subject matter; other technical subjects not mentioned herein will be clearly understood by those skilled in the art through the following description.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solutions:

[0006] Studies have shown that traditional Chinese medicines with properties of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, nourishing blood and replenishing qi, and nourishing yin and enhancing white blood have varying degrees of anti-radiation effects. Based on the combination of the traditional Chinese medicine theory of "fire toxicity" and the theory of "radiation toxicity," the inventors have made an original screening and discovered natural medicinal small molecule coltsfoot ketone, which has antioxidant, free radical scavenging, and anti-inflammatory effects, and has potential anti-radiation effects.

[0007] This invention provides the application of coltsfoot ketone, specifically its use in the preparation of drugs or products for treating and / or alleviating radiation damage.

[0008] The coltsone mentioned is a compound with CAS number 104012-37-5.

[0009] The structural formula of the coltsfoot ketone is as follows: .

[0010] In the above applications, the radiation is ionizing radiation.

[0011] In the above applications, the ionizing radiation is gamma-ray radiation.

[0012] The present invention also provides a drug for preventing radiation damage, wherein the active ingredient of the drug is coltsone.

[0013] In the aforementioned drugs, the radiation is ionizing radiation.

[0014] In the aforementioned drugs, the ionizing radiation is gamma-ray radiation.

[0015] In the aforementioned drugs, the radiation is ionizing radiation.

[0016] In the aforementioned drugs, the ionizing radiation is gamma-ray radiation.

[0017] In the above-mentioned drugs, the test animals are mammals.

[0018] In the aforementioned drugs, the mammals include mice.

[0019] The aforementioned drugs can be administered via gavage or injection.

[0020] The term "treatment" generally refers to a method implemented to achieve a beneficial or desired clinical outcome. Beneficial or desired clinical outcomes include, but are not limited to, reduction of symptoms, lessening of disease severity, reduction of disease extent, stabilization of disease (i.e., cessation of disease progression), delay or slowing of disease progression, improvement or relief of disease status (whether partial or complete remission), whether detectable or undetectable. Furthermore, treatment can also refer to an extension of survival compared to the expected survival of a subject without treatment.

[0021] In some embodiments of the invention, the animal is a mammal. Mammals are animals of the orders Primate, Ceboid, Simoid (monkeys), or Anthropoid (humans and apes).

[0022] In one specific embodiment of the present invention, the animal is a mouse.

[0023] The aforementioned anti-radiation damage drugs have the following functions: A1) can reduce alveolar congestion, hemorrhage, and inflammatory cell infiltration; A2) Relieves damage to the hepatic cord structure and congestion of the hepatic sinusoids in the liver tissue; A3) Reduce bone marrow structural disorder and vacuolation, and promote the recovery of hematopoietic cells in the bone marrow; A4) Reduces edema and shedding of intestinal villi, and repairs the integrity of the intestinal structure; A5) Improves the degree of damage to hair follicle structure and proliferation of fibrous bundles in skin tissue.

[0024] The advantages of this invention are that, through experiments, it has been demonstrated that coltsfoot ketone can effectively alleviate alveolar congestion and hemorrhage and inflammatory cell infiltration in mice after radiation; alleviate hepatic cord structure damage and hepatic sinus congestion in liver tissue; reduce bone marrow structure disorder and vacuolation, and promote the recovery of hematopoietic cells in bone marrow; reduce intestinal villi edema and shedding, and partially repair the integrity of intestinal structure; and improve hair follicle structure damage and fibrous bundle proliferation in skin tissue. Attached Figure Description

[0025] Figure 1 The effect of trogopterone administration on the survival rate of mice subjected to 8 Gy whole-body irradiation (n=10). Orange represents the control group, blue the irradiation group, red the trogopterone group, and yellow-green the soliestradiol group.

[0026] Figure 2 The effect of coltsantone administration on the quality of life of mice subjected to 8 Gy of whole-body radiation (n=10). Orange represents the control group, purple the radiation group, blue the coltsantone group, and black the soliestradiol group.

[0027] Figure 3 Gross view of mice irradiated after administration of coltsoni. From left to right, the first image is the control group, the second image is the irradiated group, the third image is the estradiol group, and the fourth image is the coltsoni group.

[0028] Figure 4 HE staining results and quantitative analysis of bone marrow tissue sections after administration of coltsfoot ketone. From left to right, the first image is the control group, the second image is the radiation group, the third image is the estradiol group, the fourth image is the coltsfoot ketone group, and the fifth image shows the quantitative analysis results of HE staining for each group.

[0029] Figure 5The results of HE staining and quantitative analysis of lung tissue pathological sections after administration of coltsfoot ketone are shown. From left to right, the first image is the control group, the second image is the radiation group, the third image is the estradiol group, the fourth image is the coltsfoot ketone group, and the fifth image shows the quantitative analysis results of HE staining for each group.

[0030] Figure 6 The results of HE staining and quantitative analysis of liver tissue sections after administration of coltsfoot ketone are shown. From left to right, the first image is the control group, the second image is the radiation group, the third image is the estradiol group, the fourth image is the coltsfoot ketone group, and the fifth image shows the quantitative analysis results of HE staining for each group.

[0031] Figure 7 HE staining results and quantitative analysis of intestinal tissue sections after administration of coltsfoot ketone. From left to right, the first image is the control group, the second image is the radiation group, the third image is the estradiol group, the fourth image is the coltsfoot ketone group, and the fifth image shows the quantitative analysis results of HE staining for each group.

[0032] Figure 8 HE staining results and quantitative analysis of skin tissue sections after administration of coltsfoot ketone. From left to right, the first image is the control group, the second image is the radiation group, the third image is the estradiol group, the fourth image is the coltsfoot ketone group, and the fifth image shows the quantitative analysis results of HE staining for each group.

[0033] Figure 9 Immunofluorescence staining results and quantitative analysis of key factors in hematopoietic repair and homeostasis maintenance in the bone marrow microenvironment after tsune administration. The first column from top to bottom shows the results of LEPR. + Cxcl12 + Percentage of CAR cells in mouse bone marrow (10X global view image), second column is lepr + Cxcl12 + Percentage of CAR cells in mouse bone marrow (20X magnified image). From left to right, the first column is the control group, the second column is the radiation group, the third column is the estradiol group, and the fourth column is the coltsfoot group.

[0034] Figure 10 for Figure 9 Quantitative analysis results for each group. Detailed Implementation

[0035] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.

[0036] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available.

[0037] The following examples used GraphPad Prism 8 statistical software to process the data. The experimental results are expressed as mean ± standard deviation. One-way ANOVA was used, and P < 0.05 was considered satisfactory. () indicates a significant difference.

[0038] Example 1: Validation of the survival rate of mice after radiation exposure by a single oral gavage administration of tsampaine. 1. Experimental Methods Coltsone source: purchased from Shanghai EFEBIO Biotechnology Co., Ltd., product number E002541.

[0039] Preparation method of coltsone solution: Mix 15% Cremophor EL (GLPBIO, GC30024) with 85% physiological saline (0.9% NaCl, sterile) (Servicebio, G4702) by shaking to prepare a solvent for subsequent use. Dissolve 12.6 mg of coltsone in 3 ml of the solvent and sonicate at 37°C for 30 minutes to aid dissolution, thereby obtaining a coltsone solution with a coltsone content of 12.6 mg / mL.

[0040] Preparation method of nystatin solution: Dissolve 0.5% sodium carboxymethyl cellulose (Solarbio, IS9000) in physiological saline solution, shake to mix, and prepare a solvent for subsequent use. Dissolve 1 mg of nystatin in 2 ml of the solvent, and sonicate at 37°C for 10 minutes to aid dissolution, and obtain nystatin solution with a content of 0.5 mg / mL.

[0041] Coltsfoot ketone is a compound with CAS number 104012-37-5, and its structural formula is as follows: .

[0042] The experimental groups are as follows: (1) Animal housing: C57BL / 6J mice, 8 weeks old, weighing 23-25g, male, were housed at the Animal Center of the Academy of Military Medical Sciences (SPF grade) after purchase. The housing temperature was 22±2℃, and the humidity was 55±5%. The bedding was sterilized by gamma rays and changed twice a week. There were 12 hours of light and 12 hours of darkness per day, with 10 mice per cage, fed standard gamma-ray sterilized feed and acidified water. All mice were housed in the animal room for one week after purchase to acclimatize to the environment before the experiment was conducted.

[0043] (2) Mouse grouping: 40 mice were divided into 4 groups of 10 each (control group, radiation group, coltsfoot ketone group, and nystatin group).

[0044] (3) 24 hours before irradiation, the coltsone group was given 200 μl of coltsone solution per animal by gavage, and the solenoid group was given 200 μl of solenoid estradiol per animal by gavage.

[0045] (4) Before irradiation, mice were placed in separate spaces to restrict their free movement.

[0046] (5) The box containing the mice was placed 2.5 meters away from the gamma-ray radiation source to receive irradiation, with a total dose of 8.0 Gy. The irradiated mice were immediately returned to the cages in the SPF barrier environment to move around and eat freely.

[0047] (6) Observe and record the survival status and weight changes of the mice daily. Survival rate = number of surviving mice in each group / total number of mice in each group × 100%.

[0048] 2. Experimental Results C57BL / 6J mice were administered different concentrations of coltsone and norethisterone via gavage 24 hours prior to irradiation, followed by whole-body irradiation with 8.0 Gy at a dose rate of 64.22 R / min. Changes in the mice's condition, body weight, and survival time were observed over 30 days. Results showed that... Figure 1 As shown, all mice in the radiation group died within 20 days after being irradiated with 8.0 Gy. Mice in the drug administration group and the positive drug control group (nystatin) were in very good condition after irradiation. The survival rate of mice in the coltsfoot ketone administration group and the positive drug control group (nystatin) was 60%.

[0049] Weight results as follows Figure 2 As shown, from day 25 to day 30, the body weight of mice in the coltsfoot ketone group was basically the same as that of mice in the control group (no statistical difference), and was much higher than that of mice in the nystatin group.

[0050] The appearance of the mouse is as follows Figure 3 As shown, it was clearly observed that after administration of coltsfoot ketone, the symptoms of ruffled fur and curled-up body in mice were significantly reduced compared to the radiation group. Therefore, coltsfoot ketone can effectively improve the overall survival of radiation-damaged animals.

[0051] Example 2: HE staining of multi-tissue repair in whole-body radiation target organs of mice. 1. Experimental Methods (1) Animal housing: C57BL / 6J mice, 8 weeks old, weighing 23-25g, male, were housed at the Animal Center of the Academy of Military Medical Sciences (SPF grade) after purchase. The housing temperature was 22±2℃, and the humidity was 55±5%. The bedding was sterilized by gamma rays and changed twice a week. There were 12 hours of light and 12 hours of darkness per day, with 10 mice per cage, fed standard gamma-ray sterilized feed and acidified water. All mice were housed in the animal room for one week after purchase to acclimatize to the environment before the experiment was conducted.

[0052] (2) Mouse grouping: 40 mice were divided into 4 groups of 10 each (control group, radiation group, coltsone group, and nystatin group).

[0053] (3) 24 hours before irradiation, the coltsone group was given coltsone solution 30 mg / kg by gavage (30 mg / kg is calculated based on coltsone), and the nystatin group was given nystatin 5 mg / kg by gavage (5 mg / kg is calculated based on nystatin).

[0054] (4) Before irradiation, mice were placed in separate spaces to restrict their free movement.

[0055] (5) The box containing the mice was placed 2.5 meters away from the gamma-ray radiation source to receive irradiation, with a total dose of 8.0 Gy. The irradiated mice were immediately returned to the cages in the SPF barrier environment to move around and eat freely.

[0056] (6) On the fifth day after radiation, the mice were sacrificed and their lungs, intestines, liver, bones, skin and other tissues were removed and fixed with 4% paraformaldehyde for 24 hours.

[0057] (7) Paraffin sample sectioning: Use a microtome to cut the paraffin sample into 4 μm thin slices, place them in a water spreader at 42℃, and after the thin slices are flattened, pick them up with a glass slide, shake off the water droplets on the glass slide, and place them on a 60℃ drying machine to bake for 40 min.

[0058] (8) Dewaxing steps: Xylene I, 8 min → Xylene II, 8 min → Xylene III, 8 min → Anhydrous ethanol 4 min → Anhydrous ethanol 4 min → 90% ethanol solution 4 min → 85% ethanol solution 4 min → 80% ethanol solution 4 min → Wash with tap water 4-5 times.

[0059] (9) Hematoxylin staining of cell nuclei: After dewaxing and rinsing with water, place the slices in hematoxylin for 5 min, rinse with water until there is no color in the water, let stand in water for 15 min, and then turn blue.

[0060] (10) Dehydration → Eosin staining of cytoplasm: 60% alcohol solution, 1 min → 70% alcohol solution, 1 min → 80% alcohol solution, 1 min → 85% alcohol solution, 1 min → 90% alcohol solution, 1 min → 95% alcohol solution, 1 min → Eosin staining, 8 s → 80% alcohol solution, 30 s → 85% alcohol solution, 1 min → 90% alcohol solution, 1 min → 95% alcohol solution, 1 min → Anhydrous ethanol, 4 min → Xylene, 4 min.

[0061] (11) After mounting with neutral resin, observe under a light microscope.

[0062] 2. Results Analysis like Figure 4 As shown, tsunemenone administration can alleviate bone marrow structural disorder and vacuolation, and promote the recovery of hematopoietic cells in the bone marrow; Figure 5 As shown, tsuneidone administration can alleviate alveolar congestion and hemorrhage, as well as inflammatory cell infiltration; Figure 6 As shown, tsuneidone administration can alleviate the damage to the hepatic cord structure and congestion of the hepatic sinusoids in liver tissue; such as Figure 7 As shown, tsunefelone administration can alleviate intestinal villus edema and shedding, and partially repair the integrity of the intestinal structure; Figure 8 As shown, administration of coltsfoot ketone can alleviate and improve the damage to hair follicle structure and the proliferation of fiber bundles in skin tissue. Figures 4-8 The scores are judged according to the scoring system in Tables 1-4 below.

[0063] Table 1. Simplified Lung Injury Pathological Scoring System (Commonly Used in Clinical Practice)

[0064] References: Matute-Bello G, Downey G, Moore BB, et al. An official AmericanThoracic Society workshop report: features and measurements of experimentalacute lung injury in animals. Am J Respir Cell Mol Biol. 2011;44(5):725-738.doi:10.1165 / rcmb.2009-0210ST. Table 2. General Semi-Quantitative Scoring System for Acute Liver Injury

[0065] References: Suzuki S, Toledo-Pereyra LH, Rodriguez FJ, Cejalvo D. Neutrophilinfiltration as an important factor in liver ischemia and reperfusion injury. Modulating effects of FK506 and cyclosporine. Transplantation. 1993;55(6):1265-1272. doi:10.1097 / 00007890-199306000-00011. Table 3. General Semi-Quantitative Scoring System for Intestinal Mucosal Injury

[0066] References: Chiu CJ, McArdle AH, Brown R, Scott HJ, Gurd FN. Intestinal mucosallesion in low-flow states. I. A morphological, hemodynamic, and metabolicreappraisal. Arch Surg. 1970;101(4):478-483. doi:10.1001 / archsurg.1970.01340280030009. Table 4. General Semi-Quantitative Scoring System for Skin Injuries

[0067] References: Singer AJ, Clark RA. Cutaneous wound healing. N Engl J Med. 1999;341(10):738-746. doi:10.1056 / NEJM199909023411006. Example 3: Immunofluorescence staining of bone marrow hematopoietic repair in mice 1. Experimental Methods (1) Animal housing: C57BL / 6J mice, 8 weeks old, weighing 23-25g, male, were housed at the Animal Center of the Academy of Military Medical Sciences (SPF grade) after purchase. The housing temperature was 22±2℃, and the humidity was 55±5%. The bedding was sterilized by gamma rays and changed twice a week. There were 12 hours of light and 12 hours of darkness per day, with 10 mice per cage, fed standard gamma-ray sterilized feed and acidified water. All mice were housed in the animal room for one week after purchase to acclimatize to the environment before the experiment was conducted.

[0068] (2) Mouse grouping: 40 mice were divided into 4 groups of 10 each (control group, radiation group, coltsone group, and nystatin group).

[0069] (3) 24 hours before irradiation, the coltsone group was given coltsone 30 mg / kg by gavage (30 mg / kg is calculated as coltsone), and the nystatin group was given nystatin 5 mg / kg by gavage (5 mg / kg is calculated as nystatin).

[0070] (4) Before irradiation, mice were placed in separate spaces to restrict their free movement.

[0071] (5) The box containing the mice was placed 2.5 meters away from the gamma-ray radiation source to receive irradiation, with a total dose of 8.0 Gy. The irradiated mice were immediately returned to the cages in the SPF barrier environment to move around and eat freely.

[0072] (6) On the fifth day after radiation, the mice were sacrificed and their lungs, intestines, liver, bones, skin and other tissues were removed and fixed with 4% paraformaldehyde for 24 hours.

[0073] (7) Paraffin sample sectioning: Use a microtome to cut the paraffin sample into 4 μm thin slices, place them in a water spreader at 42℃, and after the thin slices are flattened, pick them up with a glass slide, shake off the water droplets on the glass slide, and place them on a 60℃ drying machine to bake for 40 min. (8) Dewaxing steps: Xylene I, 8 min → Xylene II, 8 min → Xylene III, 8 min → Anhydrous ethanol 4 min → Anhydrous ethanol 4 min → 90% ethanol solution 4 min → 85% ethanol solution 4 min → 80% ethanol solution 4 min → Wash with tap water 4-5 times.

[0074] (9) Perforation: Take an appropriate amount of perforation solution and drop it onto the tissue, ensuring that it covers the tissue. Incubate at room temperature for 30 min and then discard it (shake gently). Place the slide in PBS and wash it 3 times, 5 min each time, on a shaker. (10) Antigen retrieval: Dilute the citric acid retrieval solution with pH 6.0 at a ratio of 1:10 (deionized water), immerse the slides in 400 uL of the diluted citric acid antigen retrieval solution, preheat the water bath to 95℃ and heat for 15 min, cool to room temperature after heating, place the slides in PBS and wash 3 times, 5 min each time, on a shaker. (11) Fluorescence quenching: The tissue area can be circled on the slide in advance with a histochemical pen, and the fluorescence quenching agent can be dropped onto the tissue of the slide and blocked at room temperature for 1 h; (12) Incubation with primary antibody: Discard the goat serum on the tissue, add the pre-prepared primary antibody (1:200 dilution) to the tissue, incubate overnight at 4°C (at least 8 h), take it out the next day and warm it to room temperature, place the slide in PBS and wash 3 times, 5 min each time, on a shaker; Primary antibody used: Cxcl12 (proteintech, 17402-1-AP). (13) Incubation of secondary antibody: Discard the residual PBS on the tissue, add the pre-prepared fluorescent secondary antibody (1:300 dilution) to the tissue, fully cover the tissue, incubate at room temperature in the dark for 1 h, wash with PBS 3 times, 5 min / time, and perform secondary antibody on a shaker: Alexa Fluor48 labeled goat anti-rabbit IgG (Servicebio, GB25303). (14) Repeat steps 11-13, using primary antibody: Anti-Leptin Receptor (Abcam, AB5593) and secondary antibody: Cy3-labeled goat anti-rabbit IgG (Servicebio, GB21303); (15) Fluorescence quenching: Add fluorescence quencher solution B to the slide, cover the tissue, incubate at room temperature in the dark for 5 min, and wash with PBS for 5 min; (16) Counterstaining cell nuclei with DAPI: Dilute DAPI at 1:1000, add it to the tissue, and incubate for 15-20 min; (17) The sealing procedure is the same as described above.

[0075] 2. Results Analysis Immunofluorescence staining results of key factors in hematopoietic repair and homeostasis maintenance in the bone marrow microenvironment after tsuneidone administration are as follows: Figure 9 and 10 As shown, the results indicate that, compared with the radiation group, after coltsfoot ketone intervention, the expression of key markers in CAR cells, which are involved in hematopoietic repair and homeostasis maintenance, was significantly increased, indicating that CAR cells have recovered to some extent. This suggests that coltsfoot ketone can promote the repair of the bone marrow microenvironment after radiation and accelerate the reconstruction of the body's hematopoietic function.

[0076] Example 4: Assessment of chronic hepatotoxicity and nephrotoxicity of coltsfoot 1. Experimental Methods (1) Animal housing: C57BL / 6J mice, 8 weeks old, weighing 23-25g, male, were housed at the Animal Center of the Academy of Military Medical Sciences (SPF grade) after purchase. The housing temperature was 22±2℃, and the humidity was 55±5%. The bedding was sterilized by gamma rays and changed twice a week. There were 12 hours of light and 12 hours of darkness per day, with 10 mice per cage, fed standard gamma-ray sterilized feed and acidified water. All mice were housed in the animal room for one week after purchase to acclimatize to the environment before the experiment was conducted.

[0077] (2) Mouse grouping: 30 mice were divided into 3 groups of 10 each (control group, solvent group, and drug administration group) (solvent group, 0.2 ml gavage each time) and drug administration group (the content of coltsfoot ketone in the gavage solution was 12.6 mg / ml, 0.2 ml gavage).

[0078] (3) The solvent and coltsfoot ketone group were administered once.

[0079] (4) 40 days after administration, blood was collected from the posterior orbital venous plexus and placed in an EDTA anticoagulant tube. The tube was shaken well and used for blood biochemical analysis.

[0080] 2. Results Analysis As shown in Table 5, coltsfoot ketone, which plays a therapeutic role, did not significantly induce an increase in liver and kidney damage indicators, thus ruling out liver and kidney damage caused by coltsfoot ketone within a certain dosage range.

[0081] Table 5. Effects of coltsone on the liver and kidneys

[0082] Effects of coltsone on liver and kidney function in mice (± s, n=10).

[0083] Note: ALT: alanine aminotransferase; AST: aspartate aminotransferase; ALP: alkaline phosphatase; UREA: urea; CRE: creatinine; P<0.05 compared with the control group at the same time point.

[0084] The present invention has been described in detail above. Those skilled in the art will recognize that the invention can be practiced in a wide range of ways with equivalent parameters, concentrations, and conditions without departing from its spirit and scope, and without requiring unnecessary experiments. While specific embodiments have been provided, it should be understood that further modifications can be made to the invention. In summary, according to the principles of the invention, this application is intended to include any changes, uses, or improvements to the invention, including changes made using conventional techniques known in the art that depart from the scope disclosed herein.

Claims

1. The application of coltsfoot ketone, characterized in that, The application refers to the use of coltsfoot ketone in the preparation of drugs or products for treating and / or alleviating radiation damage.

2. The application according to claim 1, characterized in that, The radiation mentioned is ionizing radiation.

3. The application according to claim 2, characterized in that, The ionizing radiation is gamma-ray radiation.

4. A drug for protecting against radiation damage, characterized in that, The active ingredient of the anti-radiation damage drug is coltsfoot ketone.

5. The drug according to claim 4, characterized in that, The radiation mentioned is ionizing radiation.

6. The drug according to claim 5, characterized in that, The ionizing radiation is gamma-ray radiation.

7. The drug according to claim 6, characterized in that, The test animals for the drug are mammals.

8. The medicament according to claim 7, characterized in that, The mammals mentioned include mice.

9. The drug according to claim 4, characterized in that, The medication can be administered via gavage or injection.

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