Polypeptide and application thereof in preparation of anti-wrinkle product

Abstract

The invention discloses a polypeptide and application thereof in preparation of anti-wrinkle products, and belongs to the technical field of polypeptides. The amino acid sequence of the polypeptide is ELDDLAER. The polypeptide can be used in the field of related cosmetics by promoting fibroblast proliferation and promoting fibroblast to secrete collagen, and meanwhile, the polypeptide can also serve as a fibroblast proliferation accelerant to promote in-vitro culture of fibroblast, so that more raw materials are provided for scientific research and application of the fibroblast and the experimental field thereof. The method has good practical application value, not only lays a foundation for research and resource development of urechis unicinctus products, but also provides a new thought for development and utilization of marine biological resources in the future.

Description

Technical Field

[0001] This invention belongs to the field of polypeptide technology, specifically relating to a polypeptide and its application in the preparation of anti-wrinkle products. Background Technology

[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] With rising living standards and an aging population, the market demand for anti-aging skincare products has increased significantly, especially for firming and anti-wrinkle products targeting skin laxity and wrinkles. The core mechanism of skin aging lies in the degeneration of the dermal structure, including a decrease in the number of fibroblasts, a decline in collagen synthesis, and the degradation of the elastic fiber network, leading to thinner skin and weakened barrier function. While current anti-wrinkle ingredients are diverse (such as glycolic acid, growth factors, and antioxidants), most suffer from limitations such as poor permeability, unstable activity, or single target. For example, traditional antioxidants like vitamin C are easily inactivated, while collagen supplements are difficult to absorb transdermally due to their large molecular weight, thus limiting their effectiveness. Therefore, developing novel active ingredients with multi-target effects, high stability, and good transdermal permeability has become an urgent industry need.

[0004] Marine biological resources, due to their unique biological activity and environmental adaptability, have become a new direction for the development of anti-wrinkle ingredients in recent years. The body wall of the sea cucumber (Echinochloa monocyclicis) is rich in collagen, polyunsaturated fatty acids, and bioactive peptides. Studies have shown that its extracts have the potential to strengthen the body, lower blood lipids, and promote tissue repair. However, current technology for the application of sea cucumbers in the skincare field is still lacking. The extraction, purification, and efficacy verification of its active ingredients face significant challenges. For example, active substances in sea cucumbers (such as peptides) are easily deactivated by environmental factors (temperature, pH). Therefore, isolating and extracting products with firming and anti-wrinkle properties from sea cucumbers is of great significance for the development of a new generation of anti-wrinkle skincare products. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a polypeptide and its application in the preparation of anti-wrinkle products. The polypeptide provided by the present invention can promote the proliferation of fibroblasts and their secretion of collagen, thus having the effects of wrinkle removal and delaying aging.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows:

[0007] In a first aspect, the present invention provides a polypeptide having the amino acid sequence shown in SEQ ID NO: 1.

[0008] A second aspect of the present invention provides a method for preparing the polypeptide described in the first aspect, comprising obtaining the polypeptide by peptide sequence coupling.

[0009] Preferably, the coupling includes sequential coupling or segmented coupling, and is prepared by solid-phase synthesis and / or liquid-phase synthesis.

[0010] A third aspect of the present invention provides the use of the polypeptide described in the first aspect in at least one of the following aspects; a1) Prepare products that promote fibroblast proliferation; a2) Prepare products that promote collagen secretion by fibroblasts; a3) Prepare anti-wrinkle products; a4) Prepare products that delay skin aging.

[0011] In some embodiments of the present invention, the fibroblasts include 3T3 fibroblasts.

[0012] In some embodiments of the present invention, the wrinkle-removing product is a skin care product, including any one or more of the following: toner, lotion, cream, gel, aerosol, soap, facial mask, essential oil, serum, facial cleanser, makeup remover, and hand sanitizer.

[0013] In some embodiments of the present invention, the content of the polypeptide in the product is 10-100 μg / mL.

[0014] In some embodiments of the present invention, the content of the polypeptide in the wrinkle-reducing product is 1-1 mg / mL.

[0015] A fourth aspect of the present invention provides a skin care product comprising the polypeptide described in the first aspect.

[0016] In some embodiments of the present invention, the concentration of the peptide in the skin care product is 1-1 mg / mL.

[0017] In some embodiments of the present invention, the skin care products include any one or more of the following: toner, lotion, cream, gel, aerosol, soap, face mask, essential oil, serum, facial cleanser, makeup remover, and hand sanitizer.

[0018] The beneficial effects of this invention are as follows: The amino acid sequence of the polypeptide provided by this invention is ELDDLAER. This polypeptide can be used in related cosmetic fields by promoting fibroblast proliferation and collagen secretion from fibroblasts. Simultaneously, the polypeptide of this invention can also serve as a fibroblast proliferation promoter to advance the in vitro culture of fibroblasts, thereby providing more raw materials for scientific research applications in fibroblasts and related experimental fields. This invention has significant practical application value, laying the foundation for the research and resource development of sea cucumber products and providing new ideas for the future development and utilization of marine biological resources. Attached Figure Description

[0019] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0020] Figure 1 Liquid chromatography of the polypeptide provided in Example 1 of the present invention.

[0021] Figure 2 This is the mass spectrum of the polypeptide provided in Example 1 of the present invention.

[0022] Figure 3 The results show the detection of the effect of the polypeptide obtained in Example 1 of this invention on the proliferation of fibroblasts.

[0023] Figure 4 The results show the detection of the effect of the polypeptide obtained in Example 1 of this invention on promoting collagen secretion by fibroblasts. Detailed Implementation

[0024] This invention discloses polypeptides and their application in the preparation of anti-wrinkle products. Those skilled in the art can refer to the content of this document and appropriately modify the process parameters to achieve the desired results. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention. The methods and applications of this invention have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.

[0025] The first technical problem to be solved by the present invention is to provide a polypeptide, namely a polypeptide with anti-wrinkle effect extracted from sea cucumber.

[0026] To solve the above problems, the first aspect of the present invention provides a technical solution: a polypeptide having the amino acid sequence shown in SEQ ID NO: 1.

[0027] The amino acid sequence of the polypeptide is ELDDLAER (SEQ ID NO: 1).

[0028] This invention provides a sea cucumber polypeptide with activity promoting the proliferation of 3T3 fibroblasts. This polypeptide can be used in related cosmetic fields by promoting the proliferation of 3T3 fibroblasts. Simultaneously, it can also serve as a fibroblast proliferation promoter to advance the in vitro culture of fibroblasts, thus providing more raw materials for scientific research and applications in fibroblasts and related experimental fields. This invention has significant practical application value, laying the foundation for the research and resource development of sea cucumber products and providing new ideas for the future development and utilization of marine biological resources.

[0029] In a second aspect, the present invention provides a method for preparing the aforementioned polypeptide, comprising obtaining the polypeptide by peptide sequence coupling.

[0030] In this invention, the coupling includes sequential coupling or segmental coupling, and is prepared using solid-phase synthesis and / or liquid-phase synthesis. As a feasible example, the peptides in this invention are prepared using a fully automated continuous microwave peptide analyzer or manual solid-phase synthesis.

[0031] A third aspect of the present invention provides the use of the said polypeptide in at least one of the following aspects; a1) Prepare products that promote fibroblast proliferation; a2) Prepare products that promote collagen secretion by fibroblasts; a3) Prepare anti-wrinkle products; a4) Prepare products that delay skin aging.

[0032] In the research and screening of anti-wrinkle cosmetic ingredients, fibroblasts have become an indispensable core in vitro model, and their application is based on profound biological rationale. Fibroblasts are core functional cells residing in the dermis of the skin, responsible for synthesizing core extracellular matrix components such as collagen, elastin, and hyaluronic acid. These substances collectively constitute the skin's "scaffold" and "reservoir," forming the material basis for maintaining skin plumpness, firmness, and elasticity. Therefore, the formation of wrinkles is essentially closely related to the decline of fibroblast function and the degradation of the synthesized matrix. Based on this principle, using fibroblasts for screening allows for direct assessment of the potential of ingredients to reverse key aspects of skin aging from the source.

[0033] Specifically, this method primarily utilizes a standardized in vitro cell experimental procedure. First, human fibroblast cell lines are cultured, and "photoaging" cell models, including those induced by ultraviolet radiation or oxidative stress, are established to simulate the physiological environment that leads to wrinkles. Then, candidate ingredients to be screened are applied to the cells at different concentrations, and their efficacy is precisely evaluated using a series of biochemical and molecular biological indicators. These key indicators mainly include: 1. Cell viability and proliferation testing to ensure the safety of the ingredients and their ability to promote the growth of healthy cells; II. Assessment of extracellular matrix synthesis capacity: This is the most crucial criterion for wrinkle-reducing efficacy. It directly demonstrates whether the ingredients can rebuild the skin's supporting structure by quantitatively detecting the content of substances such as type I collagen secreted by cells, or by detecting whether the mRNA expression level of related genes is upregulated.

[0034] Through this systematic screening system, researchers can efficiently, rapidly, and cost-effectively identify "winners" from a vast pool of candidates that effectively activate fibroblasts and promote collagen regeneration. The discovery of star ingredients such as retinol, certain peptides, and plant extracts is a testament to this approach. In conclusion, fibroblasts, as a bridge connecting chemical components and skin biological functions, offer a scientific and reliable preliminary basis for screening anti-wrinkle cosmetic ingredients due to their high targeting capabilities and clear mechanisms, greatly promoting product innovation and iteration in the anti-aging field.

[0035] Sea cucumbers are rich in various polypeptides. This invention intends to use 3T3 fibroblasts to study and confirm the wrinkle-reducing ability of the polypeptides described in the first aspect of this invention.

[0036] In this invention, the wrinkle-removing products include, but are not limited to, skin care products, including any one or more of the following: toner, lotion, cream, gel, aerosol, soap, face mask, essential oil, serum, facial cleanser, makeup remover, and hand sanitizer.

[0037] In this invention, the content of polypeptide in the product is 10-100 μg / mL.

[0038] In this invention, the content of polypeptide in the wrinkle-reducing product is 1-1 mg / mL.

[0039] In a fourth aspect, the present invention provides a skin care product comprising the polypeptide described in the first aspect.

[0040] In this invention, the concentration of the peptide in the skincare product is 1-1 mg / mL. In a specific embodiment, the concentration of the peptide in the skincare product as described above is 2 mg / mL or 6 mg / mL.

[0041] In this invention, the skin care product also includes cosmetic-acceptable excipients.

[0042] The excipients include, but are not limited to, solvents (water, ethanol, propylene glycol, butylene glycol), emulsifiers (glyceryl stearate, polysorbate, sodium lauryl sulfate), thickeners (carbomer, xanthan gum, hydroxyethyl cellulose), humectants (glycerin, hyaluronic acid, panthenol), preservatives (phenoxyethanol, parabens, chlorphenesin), antioxidants (vitamin E, tocopheryl acetate, tea polyphenols), fragrances (synthetic fragrances, natural plant essential oils), and pigments (iron oxides, CI series dyes, pearlescent pigments). pH adjusters (triethanolamine, citric acid, sodium hydroxide), film-forming agents (polyvinyl alcohol, acrylate copolymers), soothing agents (allantoin, bisabolol, dipotassium glycyrrhizate), sunscreens (titanium dioxide, zinc oxide, ethylhexyl methoxycinnamate), antistatic agents (quaternary ammonium salts, polyquaternary ammonium salts), propellants (propane, butane, dimethyl ether, used in aerosols), adsorbents (talc, kaolin, silica), defoamers (silicone compounds, polyethers), and excipients (petrolatum, paraffin wax, beeswax).

[0043] In this invention, the skin care products include, but are not limited to, any one or more of the following: toner, lotion, cream, gel, aerosol, soap, face mask, essential oil, serum, facial cleanser, makeup remover, and hand sanitizer.

[0044] In this invention, the lotion includes, but is not limited to, moisturizing lotion, oil-controlling lotion, soothing lotion, sunscreen lotion, makeup primer, repairing lotion, refreshing lotion, or restorative lotion.

[0045] In this invention, the creams and ointments include, but are not limited to, face creams, night creams, day creams, moisturizing creams, repairing creams, essence creams, anti-aging creams, sunscreens, isolation creams, BB creams, CC creams, foundation creams, concealers, contouring creams, highlighting creams, blush creams, lipsticks, lip balms, eye creams, neck creams, hand creams, body creams, body lotions, massage creams, acne creams, anti-allergy creams, and aftershave creams.

[0046] This invention provides a sea cucumber polypeptide with activity promoting the proliferation of 3T3 fibroblasts and its applications. This invention extracts a polypeptide with anti-wrinkle effects from sea cucumbers, which can be used in related cosmetic fields by promoting the proliferation of 3T3 fibroblasts. Simultaneously, the polypeptide of this invention can also be used as a fibroblast proliferation promoter to promote the in vitro culture of fibroblasts, thereby providing more raw materials for scientific research applications in fibroblasts and related experimental fields. This invention has good practical application value, which not only lays the foundation for the research and resource development of sea cucumber products, but also provides new ideas for the future development and utilization of marine biological resources.

[0047] The polypeptide provided by this invention is the preferred solution after various attempts. In the preliminary experiment, various polypeptides were tried to be extracted and separated, but the results were not as good as those of the polypeptide of this invention.

[0048] The test materials used in this invention are all commercially available products. It should be understood that, in the various embodiments of this application, the sequence numbers of the above processes do not imply a sequential order of execution. Some or all steps may be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0049] To enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with reference to specific embodiments.

[0050] Example 1 A polypeptide with fibroblast proliferation-promoting activity was synthesized using the FMOC method. The amino acid sequence of the polypeptide is ELDDLAER (SEQ ID NO.1).

[0051] like Figure 1 As shown, the synthesized peptide was subjected to liquid chromatography purity testing, and the obtained peptide was free of impurities.

[0052] like Figure 2 As shown in the mass spectrum, the peptide was successfully synthesized.

[0053] Example 2 The activity of the polypeptide obtained in Example 1 was tested.

[0054] 1. Experimental materials and equipment 1.1 Experimental Materials DMEM (Dulbecco's Modified Eagle Medium): Gibco, USA.

[0055] Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, USA).

[0056] Thiazole blue (MTT) (Sigma-Aldrich, USA).

[0057] 3T3 mouse embryonic fibroblasts: Shanghai Cell Bank, Chinese Academy of Sciences.

[0058] 1.2 Experimental Apparatus Cell culture plates (96-well, 24-well, 6-well): Nestlé.

[0059] NEO150 constant temperature water bath (Heidolph, Germany).

[0060] High-speed refrigerated centrifuge (Shanghai Lishen Scientific Instruments Co., Ltd.)

[0061] Electronic balance (Ohaus Instruments Co., Ltd.)

[0062] Analytical balance (Sartorius GmbH, Germany).

[0063] Microporous filter membrane (0.45 μm) (Sartorius GmbH, Germany).

[0064] MCO-15AC cell culture incubator (SANYO Corporation, Japan).

[0065] Bio-tek Multifunctional Microplate Reader (Bio-tek Instruments, Inc., USA).

[0066] Pipettes (2.5 μL, 10 μL, 100 μL, 200 μL, 1000 μL): eppendorf, Germany.

[0067] Blood cell counting chamber: Shanghai Qiujing Biochemical Reagent Instrument Co., Ltd.

[0068] Microplate reader: Sunrise, Tecan, Austria.

[0069] Cleanroom workbench: Ha Donglian, Beijing Donglian Haer Instrument Manufacturing Co., Ltd.

[0070] 1.3 Main reagents and preparation methods (1) 10% DMEM: Take 90 mL of basal culture medium and 10 mL of fetal bovine serum, mix them under aseptic conditions, add 1 mL of penicillin antibody and 5 mL of HEPES, dispense into containers, and store at 4°C for later use.

[0071] (2) Phosphate-buffered saline (PBS, pH=7.0): According to the preparation method of phosphate buffer as specified in the Chinese Pharmacopoeia, take 0.68 g of potassium dihydrogen phosphate, add 29.1 mL of 0.1 mol / L sodium hydroxide solution, and dilute with water to 100 mL to obtain phosphate buffer (pH 7.0).

[0072] (3) Preparation of MTT: Weigh 100 mg of MTT powder and dissolve it in 20 mL of PBS (pH 7.2). Dissolve the solution by sonication. After complete dissolution, filter the solution through a 0.22 μm disposable filter membrane to obtain a 5 mg / mL MTT staining solution. Aliquot the solution into 5 mL sterile EP tubes and store at -20°C in the dark for later use.

[0073] 2. Experimental Methods - Polypeptide-based assay to promote fibroblast proliferation 2.1 Cell resuscitation (1) Quickly remove the cell cryopreservation tube from liquid nitrogen and then rapidly immerse it in a 37°C water bath to thaw. Shake it continuously and remove it as soon as it thaws. Note that the tube opening should be placed above the water surface. The entire thawing process should be controlled within 1 minute to prevent excessive cell damage.

[0074] (2) Open the cell cryopreservation tube in a clean bench and transfer the frozen cell suspension to a 10 mL sterile centrifuge tube, and add 5 mL of DMEM medium containing 10% FBS.

[0075] (3) Centrifuge at 800 rpm for 5 min and discard the supernatant.

[0076] (4) Add 1-2 mL of complete culture medium to the centrifuge tube, mix well by blowing up and down to make a cell suspension, transfer it into a 6-well plate, and incubate at 37°C in a 5% CO2 incubator.

[0077] (5) After 24 hours, change the culture medium and continue culturing. When the adherent cells cover 80% of the bottom area of ​​a single well in a six-well plate, passage them.

[0078] 2.2 Cell passage (1) Observe the cell density under a microscope. When the cells cover about 80% of the bottom area of ​​a single well of a six-well plate, passage them.

[0079] (2) In the clean bench, discard the culture medium in the six-well plate.

[0080] (3) Add 2-3 mL of 10% DMEM and mix it by repeatedly blowing and rinsing it at the bottom of the six-well plate with a pipette to disperse it.

[0081] (4) Inoculate the cells at an appropriate cell density into another culture well.

[0082] (5) Record the cell name, passage number, operator's name and passage date, and continue to culture in a 37℃, 5% CO2 incubator.

[0083] 2.3 Study on the activity of artificially isolated and purified sea cucumber peptides against 3T3 fibroblasts by MTT assay (1) Seeding cells: Collect log phase cells, adjust the cell suspension concentration to 50,000 cells / mL, 100 μL per well, and fill the edge wells with sterile PBS.

[0084] (2) Cell culture: 5% CO2, incubated in a 37℃ incubator.

[0085] (3) Drug addition: After 24 h, the peptide was replaced with 100 μL per well at drug concentrations of 0, 10, 20, 40 and 80 µg / mL.

[0086] (4) Color development: After 48 h, add 10 μL of MTT solution to each well and incubate in an incubator for 4-6 h. Terminate the culture, discard the supernatant in the wells, and add 100 μL of DMSO to each well. Shake for 10 min to fully dissolve the crystals.

[0087] (5) Colorimetric: OD value is measured at 570 nm wavelength using an ELISA reader.

[0088] (6) Data processing and analysis.

[0089] The MTT assay was used to investigate the effect of the above-mentioned peptides on promoting fibroblast proliferation. Different concentrations of 0, 10, 20, 40, and 80 µg / mL were set up in the experiment. The results showed that the peptides significantly promoted fibroblast proliferation. Figure 3 As shown, the effect of peptides on promoting fibroblast proliferation generally increased in a dose-dependent manner with increasing concentration. At a concentration of 80 μg / mL, the data in this group showed a high degree of significance compared with the control group. Therefore, peptides have a certain promoting effect on fibroblast proliferation in the low concentration range, and the effect shows an increasing trend.

[0090] 3. Experimental Methods - Experiment on Peptides to Promote Collagen Secretion by Fibroblasts The effect of starfish enzymatic hydrolysate on collagen secretion from 3T3 fibroblasts was detected using a hydroxyproline kit. The experimental procedure is as follows: 3T3 fibroblasts achieved approximately 85% confluence. After trypsin digestion, the cells were collected, resuspended, and their density adjusted to 4 × 10⁻⁶ cells / year. 4 Cells were seeded evenly in 6-well plates at a concentration of 1 / mL. After 12 h of incubation, when the cells were fully adhered, the old culture medium was aspirated, and cell culture medium containing 0.4% FBS was added to synchronize cell growth. After 24 h of incubation, the old culture medium was aspirated, and cell culture medium containing peptide concentrations of 0, 10 ng / mL, 20 μg / mL, 40 μg / mL, and 80 μg / mL were added respectively. After 48 h of incubation, the culture medium from each well was collected, and the effect of the peptide on collagen secretion in 3T3 fibroblasts was detected using a hydroxyproline assay kit.

[0091] See results Figure 4 ,from Figure 4 It can be seen that the peptide has the activity of promoting collagen secretion in the concentration range of 20-80 μg / mL, and the activity is dose-dependent.

[0092] Example 3 A polypeptide anti-wrinkle and repairing toner, the formula of which is shown in Table 1.

[0093] Table 1. Formula of Peptide Anti-Wrinkle Repairing Toner raw material Dosage The polypeptide obtained in Example 1 0.2 g Trehalose 2.5 g Hyaluronic acid powder 0.3 g glycerin 2.5 g Jema Bp 0.2 g Rose hydrosol To 100 mL The preparation method for this polypeptide anti-wrinkle and repairing toner is as follows: After mixing the polypeptide (prepared in Example 1), trehalose, and hyaluronic acid powder, add 80 mL of rose hydrosol and stir well. Let stand for 12 h until the above components are completely dissolved. Add glycerin and Germa Bp, and then add rose hydrosol to 100 mL.

[0094] Example 4 A polypeptide wrinkle-reducing and repairing lotion, the formula of which is shown in Table 2.

[0095] Table 2. Formula of Peptide Anti-wrinkle and Repairing Lotion raw material Dosage 1% Hyaluronic Acid 15 mL Biological polysaccharide gum 6 mL Trehalose 2.5 g Grapeseed oil 10 mL Jema Bp 0.3 g The polypeptide obtained in Example 1 0.6 g emulsifier 2 g essence 1 drop purified water Add to 100 mL The preparation method for this polypeptide anti-wrinkle and repair lotion is as follows: Accurately weigh the 1% hyaluronic acid solution, bio-polysaccharide gum, trehalose, and peptides according to the above formula and place them in a beaker. Add 40 mL of purified water and stir well. Then add grape seed oil, Germa BP, and flavoring according to the formula, and add double-distilled water to 100 mL. Stir well, then add emulsifier and stir vigorously until a primary emulsion is formed. Finally, emulsify using a tissue homogenizer until a homogeneous and stable emulsion is formed. Example 5 User experience evaluation of the polypeptide anti-wrinkle and repairing toner in Example 3 For the peptide-based wrinkle-reducing and repairing toner prepared in Example 3, 2 mL of the toner was evenly applied to the face of volunteers each time. The volunteers were aged 30 to 50 years, including 75 male and 75 female volunteers. All volunteers had fine lines on their faces, with noticeable wrinkles in some areas. A toner without added peptides was used as a control. The toner was used continuously for 28 days. After 28 days, the user experience and skin texture were evaluated.

[0096] Evaluation criteria: User experience was assessed through volunteers' self-reported experiences; Skin texture was assessed through visual observation and a skin texture test. Effective results were: noticeably finer skin texture, a significant reduction in fine lines (visually observed), and shallower deep lines. Normal results were: slightly shallower skin texture and a visual reduction in wrinkles compared to before the test, but the reduction was not significant. Ineffective results were: no change in skin texture compared to before the test.

[0097] The results are shown in Table 3.

[0098] Table 3. Trial Table of Peptide Anti-Wrinkle Repairing Toner

[0099] As shown in the table above, after 28 days of use, most people reported positive results with the peptide anti-wrinkle and repair toner, which showed significant anti-wrinkle and repair effects, especially in terms of wrinkle reduction.

[0100] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A polypeptide, characterized in that, It has the amino acid sequence shown in SEQ ID NO:

1.

2. A method for preparing the polypeptide according to claim 1, characterized in that, This includes obtaining the polypeptide by coupling according to the peptide sequence; Preferably, the coupling includes sequential coupling or segmented coupling, and is prepared by solid-phase synthesis and / or liquid-phase synthesis.

3. The use of the polypeptide of claim 1 in at least one of the following aspects; a1) Prepare products that promote fibroblast proliferation; a2) Prepare products that promote collagen secretion by fibroblasts; a3) Prepare anti-wrinkle products; a4) Prepare products that delay skin aging.

4. The application as described in claim 3, characterized in that, The fibroblasts include 3T3 fibroblasts.

5. The application as described in claim 3, characterized in that, The wrinkle-reducing products are skincare products, including any one or more of the following: toner, lotion, cream, gel, aerosol, soap, mask, essential oil, serum, facial cleanser, makeup remover, and hand sanitizer.

6. The application as described in claim 3, characterized in that, The product contains 10-100 μg / mL of polypeptides.

7. The application as described in claim 3, characterized in that, The wrinkle-reducing product contains 1-1 mg / mL of polypeptides.

8. A skincare product, characterized in that, It includes the polypeptide described in claim 1.

9. The skincare product as described in claim 8, characterized in that, The concentration of peptides in the skin care product is 1-1 mg / mL.

10. The skincare product as described in claim 8, characterized in that, The skincare products include any one or more of the following: toner, lotion, cream, gel, aerosol, soap, face mask, essential oil, serum, facial cleanser, makeup remover, and hand sanitizer.

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