Cellulose enzymolysis efficient extraction and detection device for qiongzhuea tumidinoda powder

Abstract

The invention belongs to the technical field of extraction and detection, and particularly relates to a high-efficiency extraction and detection device for qiongzhuea tumidinoda powder cellulose enzymolysis, which comprises a detection table and a support frame, the detection table is fixedly connected to the support frame, and one side of the upper end surface of the detection table is provided with a high performance liquid chromatograph. The detection table is also provided with a qiongzhuodon powder pretreatment assembly; the qiongzhuodon powder pretreatment assembly comprises a mixing barrel fixedly connected to one side of the upper end face of the detection table, a barrel cover is rotationally arranged on one side of the upper end of the mixing barrel, a supporting plate is fixedly connected to the upper side of the inner cavity wall of the mixing barrel, and a throwing-out barrel is rotationally arranged in the middle of the supporting plate. The qiongzhuea tumidinoda powder pretreatment assembly is utilized to cut the agglomerated qiongzhuea tumidinoda powder before the qiongzhuea tumidinoda powder is mixed with the alkali liquor, so that the volume of the qiongzhuea tumidinoda powder is reduced, and non-uniform reaction caused by agglomeration of the qiongzhuea tumidinoda powder is avoided.

Description

Technical Field

[0001] This invention belongs to the field of extraction and detection technology, specifically a high-efficiency extraction and detection device for cellulose enzymatic hydrolysis of bamboo powder. Background Technology

[0002] Qiong bamboo powder is a powdery material made from Qiong bamboo shoots or their processing residues through pulverization. Its main components are cellulose, hemicellulose, and lignin. Qiong bamboo powder has a cellulose content as high as 40%. The glucose and cellobiose produced after enzymatic hydrolysis are key platform compounds that can be converted into bioethanol, functional oligosaccharides, and biodegradable materials. An extraction and detection device enables a one-stop, continuous, and real-time conversion from Qiong bamboo powder to quantitative data on sugar components.

[0003] In the existing technology, when performing enzymatic extraction and detection of cellulose from *Qiongzhu* powder, it is necessary to add *Qiongzhu* powder and alkaline solution to a mixing tank, stir and mix them, then perform solid-liquid separation, and finally introduce the liquid into a high-performance liquid chromatograph for analysis and detection.

[0004] However, before mixing the bamboo powder with the alkaline solution, the bamboo powder may clump or agglomerate, making it difficult to mix thoroughly and affecting the efficiency of subsequent analysis and detection. Furthermore, when the bamboo powder is added to the mixing tank, it tends to accumulate in certain areas, requiring a longer stirring time to ensure uniform mixing, thus impacting the mixing efficiency. Summary of the Invention

[0005] To overcome the shortcomings of the prior art and solve at least one of the technical problems mentioned in the background art, the present invention proposes a high-efficiency extraction and detection device for cellulose enzymatic hydrolysis of bamboo powder.

[0006] The technical solution adopted by the present invention to solve its technical problem is: a high-efficiency extraction and detection device for cellulose enzymatic hydrolysis of bamboo powder, including a detection stage and a support frame. The detection stage is fixedly connected to the support frame. A high-performance liquid chromatograph is provided on one side of the upper surface of the detection stage. A bamboo powder pretreatment component is also provided on the detection stage.

[0007] The bamboo powder pretreatment assembly includes a mixing tank fixedly connected to one side of the upper surface of the testing platform. A lid is rotatably mounted on one side of the upper end of the mixing tank. A support plate is fixedly connected to the upper side of the inner wall of the mixing tank. A discharge cylinder is rotatably mounted in the middle of the support plate. Multiple discharge holes are evenly arranged along the circumference of the discharge cylinder. A rotating cylinder is rotatably mounted in the middle of the upper end of the discharge cylinder. Multiple rows of blades are arranged on the outer wall of the rotating cylinder. Multiple rows of blades are arranged along the circumference of the inner wall of the discharge cylinder. A stirring shaft is rotatably mounted on the inner wall of the mixing tank. Multiple blades are fixedly connected to the stirring shaft.

[0008] Preferably, the mixing tank is provided with a discharge port at the bottom, the discharge port is connected to a discharge pipe, a filter box is fixedly connected to one side of the upper surface of the detection platform, one end of the discharge pipe is connected to one side of the filter box, and one side of the filter box is connected to a high performance liquid chromatograph through a connecting pipe.

[0009] Preferably, a pH detection device is fixedly connected to one side of the outer wall of the mixing tank, the detection end of the pH detection device penetrates through the mixing tank and extends into its inner cavity, and a motor is fixedly connected to one side of the outer wall of the mixing tank, the output end of the motor being fixedly connected to one end of the stirring shaft.

[0010] Preferably, a gear ring is fixedly sleeved on one side of the outer wall of the ejector cylinder, and a gear is rotatably mounted on one side of the support plate. The gear meshes with the tooth blocks on the outer ring of the gear ring. A motor is fixedly connected to one side of the support plate, and the output end of the motor is fixedly connected to the gear.

[0011] Preferably, a cover plate is rotatably provided at the middle of the lower end of the discharge cylinder, the cover plate is in contact with the outer wall of the discharge cylinder, and a feeding cover is provided at the feeding port at the upper end of the discharge cylinder.

[0012] Preferably, a motor is fixedly connected to the middle of the lower end face of the discharge cylinder, and the output end of the motor is fixedly connected to one end of the cover plate.

[0013] Preferably, a second motor is fixedly connected to the middle of the upper end face of the throwing cylinder, and the output end of the second motor is fixedly connected to the middle of the upper end of the rotating cylinder.

[0014] Preferably, it also includes an integrated cutting and unblocking component;

[0015] The integrated cutting and unblocking assembly includes two connecting plates that are slidably connected to the bottom of the inner cavity of the rotating cylinder. Multiple unblocking columns are fixedly connected to one side of the connecting plates, and the unblocking columns are slidably connected to the rotating cylinder.

[0016] Preferably, the lower end of the connecting plate has two threaded rods, both ends of which are rotatably mounted at the bottom of the rotating cylinder.

[0017] Preferably, a motor is fixedly connected to one side of the bottom of the inner cavity of the rotating cylinder, and the output ends of the motor are fixedly connected to the ends of the threaded rods on both sides respectively.

[0018] The beneficial effects of this invention are as follows:

[0019] 1. The present invention discloses a high-efficiency cellulose enzymatic hydrolysis extraction and detection device for *Bamboo shoots* powder. Utilizing a *Bamboo shoots* powder pretreatment component, it can cut up clumps of *Bamboo shoots* powder before mixing with the alkaline solution, thereby reducing the volume of the powder and preventing uneven reaction caused by clumping. Furthermore, centrifugal force can be used to eject the *Bamboo shoots* powder through the discharge port, ensuring uniform dispersion in the mixing tank until the discharge tank is empty. Because the *Bamboo shoots* powder is uniformly dispersed in all areas of the mixing tank, only a short stirring time is needed to achieve uniform mixing with the alkaline solution, thus improving mixing efficiency.

[0020] 2. The present invention discloses a high-efficiency cellulose enzymatic hydrolysis extraction and detection device for *Bamboo stalks* powder. This device utilizes an integrated cutting and unblocking assembly. A rotating drum rotates intermittently, stopping whenever a row of unblocking columns aligns with a discharge hole. At this point, a connecting plate slides at the bottom of the rotating drum's inner cavity, allowing multiple unblocking columns to pass through the corresponding discharge holes. This pushes out any *Bamboo stalks* powder blocking the discharge holes, thus preventing blockages from affecting the subsequent passage of *Bamboo stalks* powder. Attached Figure Description

[0021] The invention will now be further described with reference to the accompanying drawings.

[0022] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0023] Figure 2 This is a schematic diagram of the three-dimensional structure of the mixing tank;

[0024] Figure 3 yes Figure 2 Enlarged view of a portion of point A in the middle;

[0025] Figure 4 This is a schematic diagram of the three-dimensional structure of the rotating cylinder;

[0026] Figure 5 yes Figure 4 Enlarged view of a section at point B in the middle;

[0027] Figure 6 This is a schematic diagram of the three-dimensional structure at the unblocking column;

[0028] Figure 7 This is a schematic diagram of the three-dimensional structure of the blade.

[0029] In the diagram: 1. Detection platform; 2. High-performance liquid chromatograph; 3. Mixing tank; 4. Tank lid; 5. pH detection device; 6. Motor 1; 7. Filter box; 8. Discharge pipe; 9. Connecting pipe; 10. Discharge port; 11. Support plate; 12. Discharge cylinder; 13. Feed cover; 14. Motor 2; 15. Rotating cylinder; 16. Discharge hole; 17. Gear ring; 18. Gear; 19. Motor 3; 20. Blade 1; 21. Cover plate; 22. Motor 4; 23. Blade 2; 24. Motor 5; 25. Threaded rod; 26. Connecting plate; 27. Unblocking column; 28. Stirring shaft; 29. ​​Support frame; 30. Blade. Detailed Implementation

[0030] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] Please refer to Figures 1-7 The present invention provides a technical solution: a high-efficiency extraction and detection device for cellulose enzymatic hydrolysis of bamboo powder, including a detection stage 1 and a support frame 29. The detection stage 1 is fixedly connected to the support frame 29. A high-performance liquid chromatograph 2 is provided on one side of the upper end face of the detection stage 1. A bamboo powder pretreatment component is also provided on the detection stage 1.

[0032] The pretreatment component for bamboo powder includes a mixing tank 3 fixedly connected to one side of the upper surface of the testing platform 1. A lid 4 is rotatably mounted on one side of the upper end of the mixing tank 3. A support plate 11 is fixedly connected to the upper side of the inner wall of the mixing tank 3. A discharge cylinder 12 is rotatably mounted in the middle of the support plate 11. Multiple discharge holes 16 are evenly arranged along the circumference of the discharge cylinder 12. A rotating cylinder 15 is rotatably mounted in the middle of the upper end of the inner cavity of the discharge cylinder 12. Multiple rows of blades 23 are arranged on the outer wall of the rotating cylinder 15. Multiple rows of blades 20 are arranged along the circumference of the inner wall of the discharge cylinder 12. A stirring shaft 28 is rotatably mounted on the inner wall of the mixing tank 3. Multiple blades 30 are fixedly connected to the stirring shaft 28.

[0033] In this embodiment, as Figures 2-4 , Figure 7 As shown, the bottom of the mixing tank 3 is provided with a discharge port 10, and the discharge port 10 is connected to a discharge pipe 8. A filter box 7 is fixedly connected to one side of the upper end face of the detection platform 1. One end of the discharge pipe 8 is connected to one side of the filter box 7, and one side of the filter box 7 is connected to the high performance liquid chromatograph 2 through a connecting pipe 9.

[0034] A pH detection device 5 is fixedly connected to one side of the outer wall of the mixing tank 3. The detection end of the pH detection device 5 passes through the mixing tank 3 and extends into its inner cavity. A motor 6 is fixedly connected to one side of the outer wall of the mixing tank 3. The output end of the motor 6 is fixedly connected to one end of the stirring shaft 28.

[0035] A gear ring 17 is fixedly sleeved on one side of the outer wall of the ejector cylinder 12, and a gear 18 is rotatably mounted on one side of the support plate 11. The gear 18 meshes with the tooth blocks on the outer ring of the gear ring 17. A motor 3 19 is fixedly connected to one side of the support plate 11, and the output end of the motor 3 19 is fixedly connected to the gear 18.

[0036] A cover plate 21 is rotatably installed at the lower middle part of the discharge cylinder 12. The cover plate 21 is in contact with the outer wall of the discharge cylinder 12. A feeding cover 13 is installed at the feeding port at the upper end of the discharge cylinder 12.

[0037] A motor 22 is fixedly connected to the middle of the lower end face of the discharge tube 12, and the output end of the motor 22 is fixedly connected to one end of the cover plate 21.

[0038] A second motor 14 is fixedly connected to the middle of the upper end face of the throwing cylinder 12, and the output end of the second motor 14 is fixedly connected to the middle of the upper end of the rotating cylinder 15.

[0039] Specifically, in the existing technology, when detecting the cellulose enzymatic extraction of Qiong bamboo powder, Qiong bamboo powder and alkaline solution need to be added to the mixing tank 3, stirred and mixed, then solid-liquid separation is performed, and then the liquid is introduced into the high performance liquid chromatograph 2 for analysis and detection.

[0040] However, before mixing the bamboo powder with the alkaline solution, the bamboo powder may clump or agglomerate, making it difficult to mix thoroughly and affecting the efficiency of subsequent analysis and detection. Furthermore, when the bamboo powder is added to mixing tank 3, it tends to accumulate locally, requiring a longer stirring time to ensure uniform mixing and further impacting mixing efficiency.

[0041] Therefore, in order to solve the above problems, the working principle of this embodiment is as follows:

[0042] In the initial state, the cover plate 21 covers the multiple discharge holes 16 on the discharge cylinder 12. The barrel cover 4 is opened, and the alkali solution is added to the mixing barrel 3. Then, the feeding cover 13 is opened, and the bamboo powder is added to the discharge cylinder 12. Then, the motor 14 is started, and the rotating cylinder 15 is rotated by the motor 14. With the cooperation of the blade 20 and the blade 23, the bamboo powder is cut, which can disperse the clumps and agglomerates of bamboo powder.

[0043] Then, motor 214 is turned off, and motor 319 drives gear 18 to rotate. Under the action of gear 18 and gear ring 17, the discharge cylinder 12 rotates. When the discharge cylinder 12 reaches a certain speed, motor 422 drives cover plate 21 to rotate, so that cover plate 21 no longer covers discharge hole 16. Then, the bamboo powder inside the discharge cylinder 12 will be thrown out from discharge hole 16 under the action of centrifugal force. Then, the bamboo powder will be evenly dispersed in mixing tank 3 until the discharge cylinder 12 is emptied.

[0044] At this point, the motor 6 drives the stirring shaft 28 to rotate, and the bamboo powder and alkali solution are stirred by the blades 30. Since the bamboo powder is cut before stirring, the clumping of the bamboo powder is reduced to a large extent, avoiding uneven reaction caused by the clumping of bamboo powder. Furthermore, since the bamboo powder is evenly dispersed in various areas of the mixing tank 3, only a short stirring time is needed to make the bamboo powder and alkali solution evenly mixed, which helps to improve the mixing efficiency.

[0045] Furthermore, the pH detection device 5 monitors the pH value in real time and adjusts it to maintain the optimal pretreatment environment. After the reaction is complete, the solid-liquid mixture is discharged into the filter box 7 through the outlet 10 and the outlet pipe 8. The filter box 7 filters the solids in the solid-liquid mixture, allowing the liquid to enter the high-performance liquid chromatograph 2 for detection and analysis.

[0046] In this embodiment, as Figures 4-6 As shown, it also includes an integrated cutting and unblocking component;

[0047] The integrated cutting and unblocking assembly includes two connecting plates 26 that are slidably connected to the bottom of the inner cavity of the rotating cylinder 15. Multiple unblocking columns 27 are fixedly connected to one side of the connecting plates 26, and the unblocking columns 27 are slidably connected to the rotating cylinder 15.

[0048] The lower end of the connecting plate 26 has two threaded rods 25, and both ends of the threaded rods 25 are rotatably mounted at the bottom of the rotating cylinder 15.

[0049] A motor 24 is fixedly connected to one side of the bottom of the inner cavity of the rotating cylinder 15. The output ends of the motor 24 on both sides are fixedly connected to the ends of the threaded rods 25 on both sides.

[0050] Specifically, in the above embodiments, although the discharge hole 16 can be used in conjunction with centrifugal force to evenly disperse the bamboo powder in the mixing tank 3, the bamboo powder can also easily clog the discharge hole 16, which not only affects the addition of bamboo powder, but also affects the subsequent passage of bamboo powder due to the blockage.

[0051] Therefore, in order to solve the above problems, the working principle of this embodiment is as follows:

[0052] Motor 214 drives the rotating drum 15 to rotate intermittently, stopping whenever a row of unblocking columns 27 aligns with a discharge hole 16. At this time, motor 524 drives the threaded rods 25 on both sides to rotate simultaneously, causing the connecting plate 26 to slide at the bottom of the inner cavity of the rotating drum 15. This allows multiple unblocking columns 27 to pass through the corresponding discharge holes 16, thus pushing out the bamboo powder blocking the discharge holes 16. This prevents the bamboo powder from being blocked due to the discharge holes 16, which would otherwise affect the passage of subsequent bamboo powder.

[0053] Working principle: In the initial state, the cover plate 21 covers the multiple discharge holes 16 on the discharge cylinder 12. Open the barrel cover 4 and add the alkali solution into the mixing barrel 3. Then open the feeding cover 13 and add the bamboo powder into the discharge cylinder 12. Then start the motor 14. The motor 14 drives the rotating cylinder 15 to rotate. With the cooperation of the blade 20 and the blade 23, the bamboo powder is cut, which can disperse the clumps and agglomerates of bamboo powder.

[0054] Then, motor 214 is turned off, and motor 319 drives gear 18 to rotate. Under the action of gear 18 and gear ring 17, the discharge cylinder 12 rotates. When the discharge cylinder 12 reaches a certain speed, motor 422 drives cover plate 21 to rotate, so that cover plate 21 no longer covers discharge hole 16. Then, the bamboo powder inside the discharge cylinder 12 will be thrown out from discharge hole 16 under the action of centrifugal force. Then, the bamboo powder will be evenly dispersed in mixing tank 3 until the discharge cylinder 12 is emptied.

[0055] At this point, the motor 6 drives the stirring shaft 28 to rotate, and the bamboo powder and alkali solution are stirred by the blades 30. Since the bamboo powder is cut before stirring, the clumping of the bamboo powder is reduced to a large extent, avoiding uneven reaction caused by the clumping of bamboo powder. Furthermore, since the bamboo powder is evenly dispersed in various areas of the mixing tank 3, only a short stirring time is needed to make the bamboo powder and alkali solution evenly mixed, which helps to improve the mixing efficiency.

[0056] Furthermore, the pH detection device 5 monitors the pH value in real time and adjusts it to maintain the optimal pretreatment environment. After the reaction is complete, the solid-liquid mixture is discharged into the filter box 7 through the outlet 10 and the outlet pipe 8. The filter box 7 filters the solids in the solid-liquid mixture, allowing the liquid to enter the high-performance liquid chromatograph 2 for detection and analysis.

[0057] Motor 214 drives the rotating drum 15 to rotate intermittently, stopping whenever a row of unblocking columns 27 aligns with a discharge hole 16. At this time, motor 524 drives the threaded rods 25 on both sides to rotate simultaneously, causing the connecting plate 26 to slide at the bottom of the inner cavity of the rotating drum 15. This allows multiple unblocking columns 27 to pass through the corresponding discharge holes 16, thus pushing out the bamboo powder blocking the discharge holes 16. This prevents the bamboo powder from being blocked due to the discharge holes 16, which would otherwise affect the passage of subsequent bamboo powder.

[0058] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cellulase hydrolysis efficient extraction detection device for Qianzhu powder, comprising a detection table (1) and a support frame (29), the detection table (1) is fixedly connected to the support frame (29), characterized in that: The upper end face of the detection platform (1) is provided with a high performance liquid chromatograph (2), and the detection platform (1) is further provided with a qin bamboo powder pretreatment assembly; The qin bamboo powder pretreatment assembly comprises a mixing barrel (3) fixedly connected to one side of the upper end face of the detection platform (1), a barrel cover (4) rotatably arranged on one side of the upper end of the mixing barrel (3), a support plate (11) fixedly connected to the upper side of the inner cavity wall of the mixing barrel (3), a throwing-out cylinder (12) rotatably arranged in the middle of the support plate (11), a plurality of rows of discharge holes (16) uniformly arranged on the circumference of the throwing-out cylinder (12), a rotating cylinder (15) rotatably arranged on the upper end of the inner cavity of the throwing-out cylinder (12), a plurality of rows of blade two (23) arranged on the outer wall of the rotating cylinder (15), a plurality of rows of blade one (20) arranged on the inner cavity wall of the throwing-out cylinder (12), and a stirring shaft (28) rotatably arranged on the inner cavity wall of the mixing barrel (3), wherein a plurality of blades (30) are fixedly connected to the stirring shaft (28). 2.The cellulase hydrolysis efficient extraction and detection device for bamboo powder according to claim 1, characterized in that: The bottom of the mixing barrel (3) is provided with a discharge port (10), the discharge port (10) is communicated with a discharge pipe (8), one side of the upper end face of the detection platform (1) is fixedly connected with a filter box (7), one end of the discharge pipe (8) is communicated with one side of the filter box (7), and one side of the filter box (7) is communicated with the high performance liquid chromatograph (2) through a connecting pipe (9). 3.The cellulase hydrolysis efficient extraction and detection device for bamboo powder according to claim 1, characterized in that: The outer wall of the mixing barrel (3) is fixedly connected with a PH detection device (5), the detection end of the PH detection device (5) penetrates through the mixing barrel (3) and extends into the inner cavity thereof, the outer wall of the mixing barrel (3) is fixedly connected with a motor one (6), and one end of the stirring shaft (28) is fixedly connected with the output end of the motor one (6).

4. The cellulase hydrolysis efficient extraction detection device for Qinzhu powder according to claim 1, characterized in that: The outer wall of the throwing-out cylinder (12) is fixedly sleeved with a gear ring (17), one side of the support plate (11) is rotatably provided with a gear wheel (18), the gear wheel (18) and the gear block of the outer ring of the gear ring (17) are intermeshed, one side of the support plate (11) is fixedly connected with a motor three (19), and the output end of the motor three (19) is fixedly connected with the gear wheel (18).

5. The cellulase hydrolysis efficient extraction detection device for Qinzhu powder according to claim 1, characterized in that: The lower end of the throwing-out cylinder (12) is rotatably provided with a cover plate (21), the cover plate (21) is attached to the outer wall of the throwing-out cylinder (12), and the upper end of the throwing-out cylinder (12) is provided with a feeding cover (13). 6.The cellulase hydrolysis efficient extraction and detection device for Qianzhu powder according to claim 5, characterized in that: The lower end of the throwing-out cylinder (12) is rotatably provided with a motor four (22), and one end of the cover plate (21) is fixedly connected with the output end of the motor four (22). 7.The cellulase hydrolysis efficient extraction and detection device for Qinzhu powder according to claim 1, characterized in that: The upper end of the throwing-out cylinder (12) is rotatably provided with a motor two (14), and the upper end of the rotating cylinder (15) is fixedly connected with the output end of the motor two (14). 8.The cellulase hydrolysis efficient extraction and detection device for Qinzhu powder according to claim 1, characterized in that: It also comprises a cutting integrated dredging assembly; The cutting integrated dredging assembly comprises two connecting plates (26) slidably connected to the inner cavity bottom of the rotating cylinder (15), a plurality of dredging columns (27) fixedly connected to one side of the connecting plate (26), and the dredging columns (27) are slidably connected with the rotating cylinder (15). 9.The cellulase hydrolysis efficient extraction detection device for Qinzhu powder according to claim 8, characterized in that: The lower end of the connecting plate (26) is screwed with two threaded rods (25), and the two ends of the threaded rods (25) are rotatably arranged at the bottom of the rotating cylinder (15). 10.The cellulase hydrolysis efficient extraction detection device for Qinzhu powder according to claim 9, characterized in that: The bottom side of the inner cavity of the rotating cylinder (15) is fixedly connected to a motor five (24), and the output ends of the motor five (24) on both sides are fixedly connected to the ends of the threaded rods (25) on both sides respectively.

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