A hair loss prevention and growth composition, serum and preparation method thereof

Abstract

This invention provides a novel anti-hair loss and hair growth composition, and an essence containing anti-hair loss and hair growth effects, as well as a method for preparing the same. The anti-hair loss and hair growth composition comprises adenosine, acetyl tetrapeptide-3, and Platycladus orientalis leaf extract. The anti-hair loss and hair growth essence comprises the anti-hair loss and hair growth composition, a hair conditioning agent, a solvent, a moisturizer, a solubilizer, a preservative, a fragrance, and water. The anti-hair loss and hair growth composition or essence of this invention can promote hair growth and improve the scalp environment, thereby effectively preventing and treating hair loss problems. Furthermore, the composition or essence of this invention has no or minimal skin irritation.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, specifically to a composition, essence, and preparation method thereof that have the effects of preventing hair loss and promoting hair growth. Background Technology

[0002] With the fast pace of modern life, hair loss has gradually become a focus of social concern. In China, the number of people suffering from hair loss has exceeded 250 million, and the trend is towards affecting younger people. There are various types of hair loss, including androgenetic alopecia, alopecia areata, and telogen effluvium, among which androgenetic alopecia is the most common type.

[0003] As consumers place greater emphasis on personal image and health, and as their understanding of hair loss increases, the market demand for anti-hair loss products continues to rise. People experiencing hair loss are no longer limited to immediate results; they also prioritize safety and long-term effectiveness. When choosing anti-hair loss products, consumers tend to seek those with scientifically validated ingredients and clearly defined mechanisms of action. Furthermore, with growing health awareness, people are more willing to adopt comprehensive measures to address hair loss, including improving lifestyle habits, adjusting diet, and using suitable products.

[0004] Against this backdrop, the hair loss prevention product market presents a broad prospect for development. Consumers' demand for personalized and professional hair loss prevention solutions has driven innovation and diversification in the hair loss prevention product market. Currently, there are many types of hair loss prevention products on the market, including shampoos, hair growth serums, medications, and essences.

[0005] Existing hair loss prevention and hair growth products generally have the following shortcomings: 1. Many products on the market contain chemically synthesized substances, which can damage scalp health or cause adverse reactions with long-term use; 2. Some products have low cure rates and their effects vary from person to person; 3. Some products may cause side effects such as scalp allergies and inflammation; 4. Some chemical drugs may cause users to become dependent, and once they stop using them, the hair loss problem may worsen. Summary of the Invention

[0006] To address the above technical problems, this invention provides, on the one hand, a novel anti-hair loss and hair growth composition, and on the other hand, an essence containing anti-hair loss and hair growth effects and its preparation method. This composition is based on natural plant extracts and, through scientific formulation, aims to provide a safe and effective solution for preventing hair loss and promoting hair growth. The anti-hair loss and hair growth composition or essence of this invention can promote hair growth and improve the scalp environment, thereby effectively preventing and treating hair loss problems. Simultaneously, the composition or essence of this invention has no or minimal skin irritation.

[0007] This invention provides a hair loss prevention and hair growth composition comprising adenosine, acetyl tetrapeptide-3 and arborvitae leaf extract.

[0008] In one embodiment, the composition provided by the present invention is characterized in that the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis extract is (1-4):(1-2):(15-70); preferably, the weight ratio is (1.5-2):1:(16-50); more preferably, the weight ratio is 2:1:50, 4:1:15, 1:1:70 or 1.5:1:16.

[0009] In one embodiment, the composition provided by the present invention further includes a solvent, which is one or a combination of water, ethanol, and propylene glycol.

[0010] In one embodiment, the present invention provides a hair loss prevention and hair growth composition comprising, by weight percentage, 0.2%–0.8% adenosine, 0.1%–0.5% acetyl tetrapeptide-3, 3%–14% Platycladus orientalis leaf extract, and 85.6%–96% solvent; preferably, the content of adenosine is 0.1%, 0.2%, 0.4%, 0.5%, 0.75%, or 0.8%; preferably, the content of acetyl tetrapeptide-3 is 0.1%, 0.2%, 0.4%, or 0.5%; preferably, the content of Platycladus orientalis leaf extract is 5%–14%, specifically 3%, 5%, 8%, 10%, or 14%; preferably, the content of solvent is 85%, 85.6%, 89.4%, 90%, 95%, or 96%.

[0011] On the other hand, the present invention provides an anti-hair loss and hair growth essence, which comprises the above-mentioned anti-hair loss and hair growth composition, hair conditioning agent, solvent, moisturizer, solubilizer, preservative, fragrance and water.

[0012] In one embodiment, the essence provided by the present invention is characterized in that the essence further includes a fragrance, wherein the fragrance is selected from one or more of herbal mint fragrance, ebony rose fragrance, magnolia fragrance, gardenia fragrance, lavender fragrance, verbena fragrance and cherry blossom fragrance; preferably, the fragrance is herbal mint fragrance.

[0013] In one embodiment, the essence provided by the present invention is characterized in that the hair conditioning agent is one or more of the following: Rehmannia glutinosa root extract, Polygonum multiflorum extract, ginger extract, Centella asiatica extract, red clover flower extract, ginseng extract, licorice extract, Ligustrum lucidum extract, tea extract, and Swertia japonica extract; preferably, the hair conditioning agent is one or more of the following: Polygonum multiflorum extract, ginger extract, Centella asiatica extract, and red clover flower extract; more preferably, the hair conditioning agent is a combination of the following: Polygonum multiflorum extract, ginger extract, Centella asiatica extract, and red clover flower extract.

[0014] In one embodiment, the essence provided by the present invention is characterized in that the hair conditioning agent is a combination of Polygonum multiflorum extract, ginger extract, Centella asiatica extract and red clover flower extract, in a weight ratio of (2-6.6):(1-4.5):(1-4.5):(1-3); preferably, the weight ratio is 3:1:4.5:2.

[0015] In one embodiment, the essence provided by the present invention is characterized in that, by weight percentage, it comprises 2.65%–21.2% of an anti-hair loss and hair growth composition, 5%–18.6% of a hair conditioning agent, 30%–40% of a solvent, 3%–10% of a moisturizer, 1%–2% of a solubilizer, 0.1%–1% of a preservative, 0.01%–0.1% of a fragrance, and 33.25%–51.8% of water; preferably, the essence comprises… The hair loss prevention and hair growth promotion composition comprises 5.3% to 10.6%; more preferably, the essence comprises 9.25% to 10.6% of the hair loss prevention and hair growth promotion composition; preferably, the essence comprises 9.25% or 10.6% of the hair loss prevention and hair growth promotion composition; preferably, the water content in the essence is 33%, 33.25%, 35%, 40%, 43.85%, 45%, 45.2%, 49.15%, 50%, 51.8%, or 52%.

[0016] In one embodiment, the essence provided by the present invention is characterized in that the solvent is one or a combination of ethanol, isopropanol, n-butanol or pentanol; preferably, the solvent is ethanol.

[0017] In one embodiment, the essence provided by the present invention is characterized in that the moisturizer is one or a combination of propylene glycol, butylene glycol, glycerin, xylitol, sorbitol or hyaluronic acid; preferably, the moisturizer is one or a combination of propylene glycol or butylene glycol; more preferably, the moisturizer is a combination of propylene glycol and butylene glycol.

[0018] In one embodiment, the essence provided by the present invention is characterized in that the moisturizer is a combination of propylene glycol and butylene glycol, wherein the weight ratio of propylene glycol to butylene glycol is (0.1-90):1; further, the weight ratio of propylene glycol to butylene glycol is 2.5:1.

[0019] In one embodiment, the essence provided by the present invention is characterized in that the solubilizer is one or a combination of ethoxydiethylene glycol, sodium dodecyl sulfate, polyethylene glycol, polyacrylate or polysiloxane; preferably, the solubilizer is ethoxydiethylene glycol.

[0020] In one embodiment, the essence provided by the present invention is characterized in that the preservative is one or more of phenoxyethanol, ethylhexylglycerin, benzyl alcohol, sodium benzoate, chlorobutanol, benzalkonium chloride or benzalkonium bromide; preferably, the preservative is a combination of phenoxyethanol and ethylhexylglycerin, wherein the weight ratio of phenoxyethanol to ethylhexylglycerin is 9:1.

[0021] In one embodiment, the essence provided by the present invention, by weight percentage, comprises 5.3%–21.2% of an anti-hair loss and hair growth composition, 2%–6.6% of Polygonum multiflorum extract, 1%–4.5% of ginger extract, 1%–4.5% of Centella asiatica extract, 1%–3% of Trifolium repens flower extract, 30%–40% of ethanol, 3%–10% of propylene glycol and butylene glycol, 1%–2% of ethoxydiethylene glycol, 0.1%–1% of phenoxyethanol and ethylhexylglycerin, and 0.01%–0% of herbal peppermint fragrance. 0.1%, water 33.25% to 51.8%; further, the weight ratio of propylene glycol to butylene glycol is (0.1 to 90): 1, and the weight ratio of phenoxyethanol to ethylhexylglycerin is 9: 1; the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis extract in the anti-hair loss and hair growth composition is (1.5 to 2): 1: (16 to 50); preferably, the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis extract in the anti-hair loss and hair growth composition is 2:1:50 or 1.5:1:16.

[0022] In one embodiment, the essence provided by the present invention is characterized in that, by weight percentage, it comprises 9.25%–10.6% of an anti-hair loss and hair growth composition, 3% of Polygonum multiflorum extract, 1% of ginger extract, 4.5% of Centella asiatica extract, 2% of red clover flower extract, 30% of ethanol, 3.5% of propylene glycol and butylene glycol, 1% of ethoxydiethylene glycol, 0.5% of phenoxyethanol and ethylhexylglycerin, 0.05% of herbal peppermint fragrance, and 43.85% of water; further, the propylene glycol and butylene glycol... The weight ratio of the diol is 2.5:1, and the weight ratio of phenoxyethanol to ethylhexylglycerin is 9:1; the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis leaf extract in the anti-hair loss and hair growth composition is (1.5-2):1:(16-50); preferably, the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis leaf extract in the anti-hair loss and hair growth composition is 2:1:50 or 1.25:1:16; preferably, the content of the anti-hair loss and hair growth composition is 9.25% or 10.6%.

[0023] In some embodiments, the "preservative" of the present invention is selected from one or more combinations of phenoxyethanol, ethylhexylglycerin, benzyl alcohol, sodium benzoate, chlorobutanol, benzalkonium chloride, and benzalkonium bromide, wherein the content of the preservative is 0.1% to 1%; preferably, the content of the preservative is 0.1%, 0.2%, 0.3%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%.

[0024] In some embodiments, the "hair conditioning agent" of the present invention is selected from extracts of traditional Chinese medicines that can be used for hair loss, hair growth, and hair development. The hair conditioning agent is one or a combination of multiple selected from Rehmannia glutinosa root extract, Polygonum multiflorum extract, ginger extract, Centella asiatica extract, Trifolium repens flower extract, ginseng extract, licorice extract, Ligustrum lucidum extract, tea extract, or Swertia japonica extract. Preferably, the hair conditioning agent is one or a combination of Polygonum multiflorum extract, ginger extract, Centella asiatica extract, or Trifolium repens flower extract. More preferably, the conditioning agent is Polygonum multiflorum extract, ginger extract, Centella asiatica extract, and Trifolium repens flower extract. The content of the "hair conditioning agent" is 5% to 18.6%, preferably 5%, 10%, 12%, 15%, or 10.5%; more preferably 10.5%.

[0025] In some embodiments, the "moisturizer" of the present invention is one or a combination of propylene glycol, butylene glycol, glycerin, xylitol, sorbitol, or hyaluronic acid; preferably, the moisturizer is one or a combination of propylene glycol and butylene glycol; more preferably, the moisturizer is a combination of propylene glycol and butylene glycol; the amount of the "moisturizer" is 3% to 10%, preferably 3%, 3.5%, 5%, 6%, 7%, 8%, or 10%; more preferably 3.5%.

[0026] In some embodiments, the "solvent" of the present invention is one or a combination of ethoxydiethylene glycol, sodium dodecyl sulfate, polyethylene glycol, polyacrylate, or polysiloxane; preferably, the solubilizer is ethoxydiethylene glycol. The amount of the "solvent" is 1% to 2%, preferably 1%, 1.5%, or 2%.

[0027] In some embodiments, the "solvent" in the serum of the present invention is one or a combination of ethanol, isopropanol, n-butanol, or pentanol; preferably, the solvent is ethanol. The "solvent" content in the serum is 28% to 65%; preferably 30% to 40%, more preferably 30%, 35%, or 40%.

[0028] This invention also provides a method for preparing an essence, comprising the following steps:

[0029] (1) Weigh purified water into a homogenizer and heat it to 65±5℃. Add adenosine and stir until completely dissolved. Then cool it down to 30±5℃ and add solubilizer and preservative. Homogenize for 3-5 minutes to obtain the basic solution.

[0030] (2) Add the fragrance to the solvent, stir evenly at room temperature, then add it to the base solution and homogenize for 5-10 minutes;

[0031] (3) Add the moisturizer, arborvitae leaf extract, acetyl tetrapeptide-3 and hair conditioning agent to the solution in step (2) in sequence, homogenize at room temperature for 5 to 10 minutes to obtain the anti-hair loss and hair growth essence.

[0032] The measurement data in this invention are subject to a certain degree of error; generally, ±10% is within a reasonable error range. The error may vary depending on the context in which it is used, but this variation shall not exceed ±10%, and may be ±9%, ±8%, ±7%, ±6%, ±5%, ±4%, ±3%, ±2%, or ±1%, preferably ±5%. For example, some embodiments provide pharmaceutical compositions containing adenosine, acetyl tetrapeptide-3, arborvitae extract, humectants, solubilizers, hair conditioning agents, antioxidants, fragrances, etc., which may vary to some extent due to the presence of error, typically within ±10%. Attached image description:

[0033] Figure 1 Results of skin irritation test on rabbits Detailed implementation method:

[0034] The present invention will be explained below with reference to embodiments. Those skilled in the art will understand that the following embodiments are for illustrative purposes only and should not be considered as limiting the scope of the invention. Where specific techniques or conditions are not specified in the embodiments, they are performed according to the techniques or conditions described in the literature or according to the product instructions. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products. Specifically, the Platycladus orientalis leaf extract was purchased from Beijing Dongfang Miaosen Biotechnology Co., Ltd.; ginseng root extract, Polygonum multiflorum extract, ginger extract, Centella asiatica extract, and Trifolium repens flower extract were all purchased from Guangzhou Ganeng Biotechnology Co., Ltd.; and Arctium lappa extract was purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd. Experiment 1: The effect of the specific composition of the anti-hair loss and hair growth composition on its anti-hair loss and hair growth effect.

[0035] This invention studies the ratio of adenosine (A), acetyl tetrapeptide-3 (B), and arborvitae leaf extract (C) in an anti-hair loss and hair growth composition. The specific formulation is as follows:

[0036] Table 1. Formulas for Anti-Hair Loss and Hair Growth Compositions

[0037]

[0038] The preparation process for each example group, based on a 2kg batch, is as follows:

[0039] Example 1: Weigh 1.988 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.008 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.004 kg of acetyl tetrapeptide-3. Continue stirring at room temperature until completely dissolved and mixed evenly.

[0040] Example 2: Weigh 1.792 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.008 kg of adenosine and stir until completely dissolved. Then, cool the mixture to 30±5℃ and add 0.2 kg of Platycladus orientalis leaf extract. Continue stirring at room temperature until the mixture is homogeneous.

[0041] Example 3: Weigh 1.796 kg of purified water into a homogenizer, add 0.004 kg of acetyl tetrapeptide-3 and stir at room temperature until completely dissolved, then add 0.2 kg of Platycladus orientalis leaf extract and continue stirring at room temperature until evenly mixed.

[0042] Example 4: Weigh 1.788 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.008 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.004 kg of acetyl tetrapeptide-3. Continue stirring at room temperature until completely dissolved. Then, add 0.2 kg of Platycladus orientalis leaf extract and continue stirring at room temperature until the mixture is homogeneous.

[0043] Example 5: Weigh 1.776 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.008 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.016 kg of acetyl tetrapeptide-3. Continue stirring at room temperature until completely dissolved. Then, add 0.2 kg of Platycladus orientalis leaf extract and continue stirring at room temperature until the mixture is homogeneous.

[0044] Example 6: Weigh 1.92 kg of purified water and heat it in a homogenizer to a temperature of 65 ± 5 °C. Add 0.016 kg of adenosine and stir until completely dissolved. Then, cool the water to 30 ± 5 °C and add 0.004 kg of acetyl tetrapeptide-3. Continue stirring at room temperature until completely dissolved. Then, add 0.06 kg of Platycladus orientalis leaf extract and continue stirring at room temperature until the mixture is homogeneous.

[0045] Example 7: Weigh 1.712 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.004 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.004 kg of acetyl tetrapeptide-3. Continue stirring at room temperature until completely dissolved. Then, add 0.28 kg of Platycladus orientalis leaf extract and continue stirring at room temperature until the mixture is homogeneous.

[0046] Comparative Example 1: Weigh 1.939 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.02 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.001 kg of acetyl tetrapeptide-3. Continue stirring at room temperature until completely dissolved. Then, add 0.04 kg of Platycladus orientalis leaf extract and continue stirring at room temperature until the mixture is homogeneous.

[0047] The above composition was used to statistically analyze the hair growth and weight on the backs of mice according to the following test method, and the results are recorded in Table 3.

[0048] (1) Mouse back hair growth test

[0049] C57BL / 6J mice, aged 6-7 weeks and weighing 19-25g, were used, with an equal number of males and females. After the animal acclimatization period, the animals were anesthetized, and then the hair on the area to be shaved, parallel to the back, was removed from each mouse using a razor. After shaving, depilatory cream was applied to remove the hair, creating an observation area for hair removal. After hair removal, the depilatory cream on the back was wiped off with a water-soaked wipe, and then the area and surrounding hair were washed again with sterilized purified water. The day of hair removal was defined as (D0). Any remaining hair was removed, and it was confirmed that the mouse's hair growth was in the resting phase (the skin was pink and undamaged).

[0050] Mice were randomly assigned to two groups based on their skin condition or body weight after hair removal: a normal group (with saline solution) and experimental groups. The day after grouping, each sample was evenly applied to the hair-removed area on the back of each group of mice. The dosage for each sample group was 5 ml / kg, while the normal group received the same volume of saline solution. The medication was administered once daily for 12 consecutive days.

[0051] ① Mouse hair growth score

[0052] On day 12 of the experiment, the mice were euthanized by carbon dioxide overdose, and their backs were photographed to evaluate hair growth in the hair-removed areas.

[0053] Table 2 Scoring criteria for mouse hair growth

[0054] score Meaning 0 points less than 20% growth 1 point Greater than 20% and less than 40% 2 points Growth rate greater than 40% but less than 60% 3 points Growth rate greater than 40% but less than 60% 4 points 60% to 80% growth 5 points 80%–100% growth

[0055] ② Mouse hair weight

[0056] After photographing the mice, the hair in the shaved area on the back of each mouse was shaved off with a razor, and the average value was calculated by weighing the shaved area using a 0.1 oz electronic balance.

[0057] Table 3. Hair growth results in mice of each example group.

[0058]

[0059]

[0060] As shown in Table 3, ① compared with the saline group, the hair growth score and hair weight of mice in each example group increased, indicating that the composition based on adenosine, acetyl tetrapeptide-3 and Platycladus orientalis leaf extract has the effect of promoting hair growth; ② when adenosine, acetyl tetrapeptide-3 and Platycladus orientalis leaf extract are used together, the hair growth effect is better than that of adenosine + acetyl tetrapeptide-3, adenosine + Platycladus orientalis leaf extract, and acetyl tetrapeptide-3 + Platycladus orientalis leaf extract; ③ when adenosine, acetyl tetrapeptide-3 and Platycladus orientalis leaf extract are used together, the ratio of (1-4):1:(15-70) can show the optimal effect of promoting hair growth. That is, in the anti-hair loss and hair growth composition containing these three components, the ratio of adenosine and Platycladus orientalis leaf extract within a certain range will not affect the effect of promoting hair growth, but increasing the ratio of acetyl tetrapeptide-3 will reduce the effect of promoting hair growth; ④ compared with Comparative Example 1, the anti-hair loss and hair growth composition of the present invention can show a better effect of promoting hair growth. Therefore, it was determined that when adenosine, acetyl tetrapeptide-3 and arborvitae leaf extract were in a specific ratio range (1-4):1:(15-70), they exhibited a superior effect in promoting hair growth.

[0061] Experiment 2: The effect of the amount of anti-hair loss and hair growth composition added to the anti-hair loss and hair growth essence on the hair growth effect.

[0062] The weight ratio of the anti-hair loss and hair growth composition was studied using only the weight ratio of the other components as a single variable. Simultaneously, a comparative example was selected using a hair growth-promoting composition comprising adenosine, acetyl tetrapeptide-3, tripeptide-1 copper, arborvitae leaf extract, ginseng root extract, and burdock extract, with a ratio of adenosine:acetyl tetrapeptide-3:tripeptide-1 copper:arborvitae leaf extract: ginseng root extract: burdock extract of 1:0.05:0.05:2:2:2. The specific anti-hair loss and hair growth essence used in the experiment comprised the components in the following weight ratios.

[0063] Table 4. Prescription for Anti-Hair Loss and Hair Growth Essence

[0064]

[0065] Example 8: Preparation process in batches of 10 kg:

[0066] (1) Weigh 5.18 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.01 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.1 kg of ethoxydiethylene glycol, 0.05 kg of phenoxyethanol and ethylhexylglycerol. Homogenize for 3-5 min to obtain the basic solution.

[0067] (2) Add 0.005 kg of herbal peppermint essence to 3 kg of ethanol, stir evenly at room temperature, then add to the base solution and homogenize for 5-10 min;

[0068] (3) Then add 0.25 kg of propylene glycol, 0.1 kg of butylene glycol, 0.25 kg of arborvitae leaf extract, 0.005 kg of acetyl tetrapeptide-3, 0.3 kg of Polygonum multiflorum extract, 0.1 kg of ginger extract, 0.45 kg of Centella asiatica extract, and 0.2 kg of red clover flower extract to the solution in step (2) in sequence, and homogenize at room temperature for 5-10 minutes to obtain the anti-hair loss and hair growth essence.

[0069] Example 9: Preparation process in batches of 10 kg:

[0070] (1) Weigh 4.915 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.02 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.1 kg of ethoxydiethylene glycol, 0.05 kg of phenoxyethanol and ethylhexylglycerol. Homogenize for 3-5 min to obtain the basic solution.

[0071] (2) Add 0.005 kg of herbal peppermint essence to 3 kg of ethanol, stir evenly at room temperature, then add to the base solution and homogenize for 5-10 min;

[0072] (3) Then add 0.25kg of propylene glycol, 0.1kg of butylene glycol, 0.5kg of arborvitae leaf extract, 0.01kg of acetyl tetrapeptide-3, 0.3kg of Polygonum multiflorum extract, 0.1kg of ginger extract, 0.45kg of Centella asiatica extract and 0.2kg of red clover flower extract to the solution in step (2) in sequence, and homogenize at room temperature for 5-10 minutes to obtain the anti-hair loss and hair growth essence.

[0073] Example 10: Preparation process in batches of 10 kg:

[0074] (1) Weigh 4.385 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.04 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.1 kg of ethoxydiethylene glycol, 0.05 kg of phenoxyethanol and ethylhexylglycerol. Homogenize for 3-5 min to obtain the basic solution.

[0075] (2) Add 0.005 kg of herbal peppermint essence to 3 kg of ethanol, stir evenly at room temperature, then add to the base solution and homogenize for 5-10 min;

[0076] (3) Then add 0.25 kg of propylene glycol, 0.1 kg of butylene glycol, 1 kg of arborvitae leaf extract, 0.02 kg of acetyl tetrapeptide-3, 0.3 kg of Polygonum multiflorum extract, 0.1 kg of ginger extract, 0.45 kg of Centella asiatica extract, and 0.2 kg of red clover flower extract to the solution in step (2) in sequence, and homogenize at room temperature for 5 to 10 minutes to obtain the anti-hair loss and hair growth essence.

[0077] Example 11: Preparation process in batches of 10 kg:

[0078] (1) Weigh 3.325 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.08 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.1 kg of ethoxydiethylene glycol, 0.05 kg of phenoxyethanol and ethylhexylglycerol. Homogenize for 3-5 min to obtain the basic solution.

[0079] (2) Add 0.005 kg of herbal peppermint essence to 3 kg of ethanol, stir evenly at room temperature, then add to the base solution and homogenize for 5-10 min;

[0080] (3) Then add 0.25kg of propylene glycol, 0.1kg of butylene glycol, 2kg of arborvitae leaf extract, 0.04kg of acetyl tetrapeptide-3, 0.3kg of Polygonum multiflorum extract, 0.1kg of ginger extract, 0.45kg of Centella asiatica extract, and 0.2kg of red clover flower extract to the solution in step (2) in sequence, and homogenize at room temperature for 5-10 minutes to obtain the anti-hair loss and hair growth essence.

[0081] Example 12: Preparation process in 10kg batches:

[0082] (1) Weigh 4.52 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.075 kg of adenosine and stir until completely dissolved. Then, cool the water to 30±5℃ and add 0.1 kg of ethoxydiethylene glycol, 0.05 kg of phenoxyethanol and ethylhexylglycerol. Homogenize for 3-5 min to obtain the basic solution.

[0083] (2) Add 0.005 kg of herbal peppermint essence to 3 kg of ethanol, stir evenly at room temperature, then add to the base solution and homogenize for 5-10 min;

[0084] (3) Then add 0.25kg of propylene glycol, 0.1kg of butylene glycol, 0.8kg of arborvitae leaf extract, 0.05kg of acetyl tetrapeptide-3, 0.3kg of Polygonum multiflorum extract, 0.1kg of ginger extract, 0.45kg of Centella asiatica extract and 0.2kg of red clover flower extract to the solution in step (2) in sequence, and homogenize at room temperature for 5-10 minutes to obtain the anti-hair loss and hair growth essence.

[0085] Comparative Example 2: Preparation process in 10kg batches:

[0086] (1) Weigh 4.735 kg of purified water and heat it in a homogenizer to a temperature of 65±5℃. Add 0.1 kg of adenosine and stir until completely dissolved. Then cool the water to 30±5℃ and add 0.1 kg of ethoxydiethylene glycol, 0.05 kg of phenoxyethanol and ethylhexylglycerol. Homogenize for 3-5 min to obtain the basic solution.

[0087] (2) Add 0.005 kg of herbal peppermint essence to 3 kg of ethanol, stir evenly at room temperature, then add to the base solution and homogenize for 5-10 min;

[0088] (3) Then add 0.25kg of propylene glycol, 0.1kg of butylene glycol, 0.2kg of arborvitae leaf extract, 0.005kg of acetyl tetrapeptide-3, 0.005kg of tripeptide-1 ketone, 0.2kg of ginseng root extract, 0.2kg of burdock extract, 0.3kg of Polygonum multiflorum extract, 0.1kg of ginger extract, 0.45kg of Centella asiatica extract, and 0.2kg of red clover flower extract to the solution in step (2) in sequence, and homogenize at room temperature for 5-10 minutes to obtain the anti-hair loss and hair growth essence.

[0089] Mouse back hair growth test

[0090] Referring to the mouse back hair growth test method described in Experiment 1, the mouse back hair growth and average hair weight of Examples 8-12 and Comparative Example 2 were statistically analyzed, as shown in Table 8.

[0091] Human efficacy trial for preventing hair loss and promoting hair growth

[0092] Sixty patients aged 20-60 years with hair loss were recruited, with an equal number of men and women. The anti-hair loss and hair growth essences of Examples 8-12 and Comparative Example 2 were used as test drugs. Patients applied 1-2 ml of the test drug evenly to the affected area twice daily (once in the morning and once in the evening) for 16 weeks. The overall effective rate (including markedly effective and effective) of the clinical treatment in the 60 patients is shown in Table 8.

[0093] Table 5 Evaluation Criteria for Human Efficacy Testing

[0094]

[0095] Rabbit skin irritation test

[0096] Eighteen New Zealand rabbits were selected and divided into six groups of three. The backs of all animals were shaved, and 2.5cm x 2.5cm areas were marked on both sides of the spine as experimental observation areas. The essences from Examples 8-12 and Comparative Example 2 were used as samples. 200µl of the test substance was evenly applied to the marked area on the right side, and physiological saline was applied to the opposite side. The dressing was applied and fixed for 4 hours, then washed with warm water. After 1 hour of recovery, observation and photography were performed, and scores were calculated according to the "Technical Guidelines for the Study of Drug Irritation, Allergy, and Hemolysis." The test substance was administered once daily for one week. The skin irritation status of the rabbits after one week is shown in the appendix. Figure 1 The skin irritation intensity score is shown in Table 8.

[0097] Table 6. Scoring Criteria for Skin Irritation Response

[0098]

[0099]

[0100] Table 7. Evaluation Criteria for Skin Irritation Intensity

[0101] Score evaluate 0-0.49 Non-irritating 0.5-2.99 Mild irritation 3.0-5.99 moderate irritation 6.0-8.0 Strong irritant

[0102] Table 8. Statistical analysis of the results of the composition addition amount.

[0103]

[0104] The results showed that: ① Mouse back hair growth test: With the increase of the amount of anti-hair loss and hair growth composition added, the hair growth score and hair weight of mice first increased and then stabilized. When the amount of anti-hair loss and hair growth composition added to the anti-hair loss and hair growth essence was 9.25% to 10.6%, the effect on promoting hair growth was the best. After reaching this dose, when the amount of anti-hair loss and hair growth composition was increased to 21.2%, the changes in hair growth and hair weight were not significant. ② Human efficacy test: When the amount of anti-hair loss and hair growth composition added was 2.65% to 10.6%, the human efficacy rate gradually increased with the increase of the composition addition. The effect was best when the composition addition reached 9.25% to 10.6%. Further increases in the composition addition rate maintained stable human efficacy. ③ Rabbit skin irritation test: The results of the rabbit skin irritation test show that when the amount of the anti-hair loss and hair growth composition added is 2.65% to 10.6%, there is no irritation; when the amount of the anti-hair loss and hair growth composition added is 21.2%, there is mild irritation. ④ Compared with Comparative Example 2, the essence of this invention has a better effect on promoting hair growth and is less irritating. In summary, considering the hair growth effect and the safety of the product, the content of the anti-hair loss and hair growth composition is preferably 9.25% to 10.6%.

[0105] In the description of this specification, the terms "one embodiment," "some embodiments," "example," "specific embodiment," or "some embodiments," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example that include at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, those skilled in the art can combine or combine features of different embodiments or examples described in this specification without contradiction.

Claims

1. A hair loss prevention and hair growth composition comprising adenosine, acetyl tetrapeptide-3 and arborvitae leaf extract, wherein the weight ratio of adenosine:acetyl tetrapeptide-3:arborvitae leaf extract is (1-4):(1-2):(15-70); preferably the weight ratio is (1.5-2):1:(16-50).

2. The composition according to claim 1, further comprising a solvent, said solvent being one or a combination of two of water, ethanol, or propylene glycol.

3. The composition according to claim 2, by weight percentage, comprises 0.2% to 0.8% adenosine, 0.1% to 0.5% acetyl tetrapeptide-3, 3% to 14% Platycladus orientalis leaf extract, and 85.6% to 96% solvent.

4. A hair loss prevention and hair growth essence, comprising the hair loss prevention and hair growth composition of claim 1, a hair conditioning agent, a solvent, a moisturizer, a solubilizer, a preservative, a fragrance, and water.

5. The essence according to claim 4, characterized in that, The essence also includes a fragrance, which is selected from one or more of herbal mint fragrance, ebony rose fragrance, magnolia fragrance, gardenia fragrance, lavender fragrance, verbena fragrance and cherry blossom fragrance; preferably, the fragrance is herbal mint fragrance.

6. The essence according to claim 4, characterized in that, The hair conditioning agent is one or more of the following: Rehmannia glutinosa root extract, Polygonum multiflorum extract, ginger extract, Centella asiatica extract, red clover flower extract, ginseng extract, licorice extract, Ligustrum lucidum extract, tea extract, or Swertia japonica extract; preferably, the hair conditioning agent is one or more of the following: Polygonum multiflorum extract, ginger extract, Centella asiatica extract, or red clover flower extract; more preferably, the hair conditioning agent is a combination of Polygonum multiflorum extract, ginger extract, Centella asiatica extract, and red clover flower extract.

7. The essence according to claim 6, characterized in that, The hair conditioning agent is a combination of Polygonum multiflorum extract, ginger extract, Centella asiatica extract, and Trifolium repens flower extract, in a weight ratio of (2–6.6):(1–4.5):(1–4.5): (1~3); preferably, the weight ratio is 3:1:4.5:

2.

8. The essence according to claim 4, characterized in that, By weight percentage, it comprises 2.65%–21.2% of an anti-hair loss and hair growth composition, 5%–18.6% of a hair conditioning agent, 30%–40% of a solvent, 3%–10% of a moisturizer, 1%–2% of a solubilizer, 0.1%–1% of a preservative, 0.01%–0.1% of a fragrance, and 33.25%–51.8% of water; preferably, the serum comprises 5.3%–10.6% of the anti-hair loss and hair growth composition; more preferably, the serum comprises 9.25%–10.6% of the anti-hair loss and hair growth composition.

9. The essence according to claim 4, characterized in that, The solvent is one or a combination of ethanol, isopropanol, n-butanol or pentanol; preferably, the solvent is ethanol.

10. The essence according to claim 4, characterized in that, The moisturizer is one or a combination of propylene glycol, butylene glycol, glycerin, xylitol, sorbitol or hyaluronic acid; preferably, the moisturizer is one or a combination of propylene glycol or butylene glycol; more preferably, the moisturizer is a combination of propylene glycol and butylene glycol.

11. The serum according to claim 10, characterized in that, The humectant is a combination of propylene glycol and butylene glycol, wherein the weight ratio of propylene glycol to butylene glycol is (0.1-90):1; further, the weight ratio of propylene glycol to butylene glycol is 2.5:

1.

12. The essence according to claim 4, characterized in that, The solubilizer is one or a combination of ethoxydiethylene glycol, sodium dodecyl sulfate, polyethylene glycol, polyacrylate, or polysiloxane; preferably, the solubilizer is ethoxydiethylene glycol.

13. The essence according to claim 4, characterized in that, The preservative is one or more of phenoxyethanol, ethylhexylglycerin, benzyl alcohol, sodium benzoate, chlorobutanol, benzalkonium chloride, or benzalkonium bromide; preferably, the preservative is a combination of phenoxyethanol and ethylhexylglycerin, wherein the weight ratio of phenoxyethanol to ethylhexylglycerin is 9:

1.

14. The serum according to claims 4-13, characterized in that, By weight percentage, it contains 5.3%–21.2% anti-hair loss and hair growth composition, 2%–6.6% Polygonum multiflorum extract, 1%–4.5% ginger extract, 1%–4.5% Centella asiatica extract, 1%–3% red clover flower extract, 30%–40% ethanol, 3%–10% propylene glycol and butylene glycol, 1%–2% ethoxydiethylene glycol, 0.1%–1% phenoxyethanol and ethylhexylglycerin, 0.01%–0.1% herbal peppermint fragrance, and 33.25% water. ~51.8%; further, the weight ratio of propylene glycol to butylene glycol is (0.1~90):1, and the weight ratio of phenoxyethanol to ethylhexylglycerin is 9:1; the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis extract in the anti-hair loss and hair growth composition is (1.5~2):1:(16~50); preferably, the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis extract in the anti-hair loss and hair growth composition is 2:1:50 or 1.5:1:

16.

15. The serum according to claim 14, characterized in that, The hair loss prevention and hair growth composition comprises, by weight percentage: 9.25%–10.6% of Polygonum multiflorum extract, 3% of ginger extract, 1% of centella asiatica extract, 4.5% of red clover flower extract, 2% of ethanol, 30% of propylene glycol and butylene glycol, 1% of ethoxydiethylene glycol, 0.5% of phenoxyethanol and ethylhexylglycerin, 0.05% of herbal peppermint fragrance, and 43.85%–45.2% of water; further, the weight ratio of propylene glycol to butylene glycol is 2.5:1, and the weight ratio of phenoxyethanol to ethylhexylglycerin is 9:1; the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis leaf extract in the hair loss prevention and hair growth composition is (1.5–2):1:(16–50); preferably, the weight ratio of adenosine:acetyl tetrapeptide-3: Platycladus orientalis leaf extract in the hair loss prevention and hair growth composition is 2:1:50 or 1.5:1:

16.

16. A method for preparing the serum according to claims 4-15, comprising the following steps: (1) Weigh purified water into a homogenizer and heat it to 65±5℃. Add adenosine and stir until completely dissolved. Then cool it down to 30±5℃ and add solubilizer and preservative. Homogenize for 3-5 minutes to obtain the basic solution. (2) Add the fragrance to the solvent, stir evenly at room temperature, then add it to the base solution and homogenize for 5-10 minutes; (3) Add the moisturizer, arborvitae leaf extract, acetyl tetrapeptide-3 and hair conditioning agent to the solution in step (2) in sequence, homogenize at room temperature for 5 to 10 minutes to obtain the anti-hair loss and hair growth essence.

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