A near-infrared fluorescent probe, its preparation method and application

By introducing alkyne functional groups into heptamethine dye derivatives and using click chemistry bridging, near-infrared fluorescent probes were prepared, solving the challenges of tumor-targeted design and tumor heterogeneity diagnosis using heptamethine dyes. This approach integrates high-sensitivity tumor imaging and in-depth proteomics analysis, providing a powerful research and diagnostic platform.

CN122079855APending Publication Date: 2026-05-26JILIN UNIV FIRST HOSPITAL
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Patent Information

Application Number
CN202610525361.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-20
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing heptamethrin-based near-infrared fluorescent dyes, such as IR-780, lack systematic mechanistic studies on tumor targeting, which limits their rational design and optimization. They cannot simultaneously achieve high-sensitivity tumor imaging and in-depth proteomics analysis, and are prone to missed detections and false negatives in heterogeneous tumor environments.

Method used

By introducing alkynyl functional groups into heptamethine dye derivatives, click chemistry bridging was used to facilitate the enrichment and identification of covalently targeted proteins, and near-infrared fluorescent probes were prepared. Combined with click chemistry reaction and efficient covalent linkage with azide labeling, high-sensitivity tumor imaging and in-depth proteomics analysis were achieved.

Benefits of technology

It integrates highly sensitive tumor imaging and deep proteomics analysis, overcoming the diagnostic instability caused by unknown targets and tumor heterogeneity in existing technologies, and providing a powerful research and diagnostic platform.

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Abstract

This invention relates to the field of molecular probe technology, and more particularly to a near-infrared fluorescent probe, its preparation method, and its applications. The near-infrared fluorescent probe provided by this invention has the structure shown in Formula I. Through click chemical bridging, it enables convenient enrichment and identification of covalently targeted proteins, thereby simultaneously achieving high-sensitivity tumor imaging and in-depth proteomics analysis. The near-infrared fluorescent probe provided by this invention not only retains and optimizes the high-contrast tumor imaging capabilities of IR-780, but also serves as a highly efficient chemical proteomics tool, systematically revealing its target map and binding mechanism. Ultimately, it forms a platform technology integrating bright pan-tumor labeling and functional proteomics analysis, providing a solution to overcome tumor heterogeneity and advance the rational design of probes.
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Citation Information

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