Functional cosmetic composition containing stem cell culture solution and method for preparing the same

Abstract

The present application provides a functional cosmetic composition containing stem cell culture solution and a preparation method thereof, wherein the functional cosmetic composition contains, based on 100 parts by weight of stem cell culture solution from one source selected from the group consisting of human adipose-derived stem cells, bone marrow, umbilical cord blood, peripheral blood, and adipose tissue base, 30 to 40 parts by weight of purified water, 1 to 5 parts by weight of glycolic acid, 3 to 7 parts by weight of trehalose, 5 to 20 parts by weight of serine, 1 to 3 parts by weight of d-panthenol, 1 to 5 parts by weight of organic sulfur, 1 to 5 parts by weight of methionine liquid, 0.3 to 1 parts by weight of hemp seed oil, and 0.1 to 0.5 parts by weight of aromatic oil.

Description

Technical Field

[0001] This invention relates to functional cosmetic compositions containing stem cell culture medium and methods for manufacturing the same, and more specifically to functional cosmetic compositions including stem cell culture mediums that provide excellent anti-aging, wrinkle-improving, moisturizing, skin-tone-improving, and elasticity-improving effects. Background Technology

[0002] Recently, stem cell technology has been opening a new chapter in the treatment of intractable diseases. Previously, organ transplantation and gene therapy were proposed to treat intractable human diseases, but their effective practical application has been slow due to immune rejection, insufficient donor organs, vector development, and insufficient knowledge of disease genes. In response, pluripotent stem cells, capable of forming all organs through proliferation and differentiation, have attracted much attention. It is believed that stem cells can not only fundamentally solve the treatment of most diseases but also fundamentally address organ damage. Many scientists have proposed various possibilities for stem cell application in treating almost all organ regeneration in the human body, as well as intractable diseases such as Parkinson's disease, various cancers, diabetes, and spinal cord injuries.

[0003] These adult stem cells were initially isolated from adult bone marrow (Y. Jiang et al., Nature, 418:412002), and have since been identified in several other adult tissues (CM Verfailie, Trends Cell Biol., 12:502, 2002). In other words, bone marrow is the most well-known source of stem cells, but pluripotent stem cells can also be obtained from adipose tissue, umbilical cord, skin, blood vessels, muscle, and the brain (JGToma et al., Nat. Cell Biol., 3:7782001; M. Sampaolesi et al., Science, 301:4872003; Y. Jiang et al., Exp. Hematol., 30:8962002). An important point for understanding adult stem cells is that they exist in small numbers in every tissue. They remain dormant for many years, without division or proliferation, before being activated by tissue disease or damage. Scholars are studying how to use cell culture to proliferate adult stem cells and induce their differentiation into specific cells that could harm or cause disease in our bodies.

[0004] Moreover, stem cells are cells that can self-replicate and differentiate into various cell types. They can be used in regenerative medicine, tissue engineering, and cell therapy, and recently their usefulness and applications are expanding into plastic and cosmetic surgery.

[0005] Recently, in the cosmetics market, stem cell cosmetics with therapeutic concepts have been developed by combining technologies that utilize growth factors derived from stem cells, proposing a new approach to address various skin problems arising from skin regeneration.

[0006] These traditional documents include Korean Patent Publication No. 10-2010-0036389. This previously disclosed patent reveals that the culture medium obtained from culturing stem cells has a significant effect on improving skin wrinkles or delaying aging.

[0007] In addition, Republic of Korea Patent No. 10-1764614 also manufactured human body fluid containing extracellular matrix components—extracellular matrix simulated water, and released a cosmetic composition for improving the skin barrier containing extracellular matrix components.

[0008] However, these previous studies only contain stem cell culture medium as an active ingredient, which has the effects of preventing aging and improving wrinkles, but does not have functions such as moisturizing or improving skin tone. In addition, the characteristic odor of stem cell culture medium is unpleasant to use.

[0009] Existing technical documents

[0010] Patent documents

[0011] Patent Document 1: Republic of Korea Patent No. 10-1764614 Summary of the Invention

[0012] The problem that the invention aims to solve

[0013] The present invention aims to solve the aforementioned problems and its purpose is to provide a functional cosmetic composition, including a culture medium for stem cells, which can have good effects in preventing aging, improving wrinkles, moisturizing, improving skin tone, and improving elasticity.

[0014] The problems to be solved by the present invention are not limited to those mentioned above, and other problems not mentioned can be clearly understood by those skilled in the art from the following description.

[0015] means for solving problems

[0016] To address the aforementioned problems, the functional cosmetic composition containing stem cell culture medium and its preparation method according to the present invention are as follows: 100 parts by weight of stem cell culture medium from one source selected from a group consisting of human adipose stem cells, bone marrow, umbilical cord blood, peripheral blood, and adipose tissue; 30 to 40 parts by weight of purified water; 1 to 5 parts by weight of prickly ash; 3 to 7 parts by weight of thiamethoxam; 1 to 5 parts by weight of serine; 1 to 5 parts by weight of serine; 0.3 to 1 part by weight of ham seed oil; and 0.1 to 0.5 parts by weight of essential oil. The stem cell culture medium contains peptides and proteins, PDGF (deformation growth factor), and basic... The ham seed oil comprises FGF (basic fibroblast growth factor), KGF (keratinocyte growth factor), TGF-β1 (including deformable growth factor), HGF (hepatocyte growth factor), VEGF (vascular endothelial growth factor), collagen, folate, and SOD (antioxidant enzyme). It also includes CBD (cannabidiol) extracted from ham root or stem, cypress extract, 0.05 to 0.1% by weight of ethanol, and arginine lactone lactone lactone lactone containing 0. Part 1, the cypress extract contains 10 stages of white debris from a pulverizing process and a washing liquid by weight, a stage of heating in water to 50 degrees Celsius, a stage of removing the heated cypress from the water, a stage of sealing the water-removed cypress in a plastic or plastic bucket to form moisture, a stage of reheating the water-removed cypress to form moisture, a stage of mixing the heated cypress water and the reheated cypress water, and is manufactured by distilling the mixed water. The essential oil includes a culture medium of stem cells, of which at least one is derived from lavender, tea tree, geranium, and mazu blue.

[0017] In cosmetic compositions prepared using the culture medium of the aforementioned stem cells, a composite solution of extracts from peppermint and oregano mixed in a 1:1 weight ratio may be added.

[0018] Pumpkin seed powder can be added to a prepared cosmetic composition using the stem cell culture medium containing the composite solution.

[0019] The pumpkin seed powder may include the following steps: washing the pumpkin seeds; drying the washed pumpkin seeds on a dry towel for 5 hours; soaking the dried pumpkin seeds in Nipah palm flower stem extract for 16 to 24 hours; drying the pumpkin seeds soaked in Nipah palm flower stem extract at room temperature for 7 to 10 hours; pulverizing the dried pumpkin seeds into 15 to 30 μm particles; and containing stem cell culture medium.

[0020] The Nipah palm flower stem extract may include a stem cell culture medium, i.e., a stage of hot water extraction using cut Nipah palm flower stems as an aqueous solvent and a stage of concentrated extraction of the extract.

[0021] Invention Effects

[0022] Similarly, this invention has good anti-aging, wrinkle-improving, moisturizing, skin-improving, and elasticity-improving effects.

[0023] In addition, it contains cypress extract, essential oil, etc., which can prevent users from having an aversion to the unique odor of stem cell culture medium.

[0024] In addition, it contains pumpkin seed powder, which has an exfoliating effect.

[0025] The effects of the present invention are not limited to those mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the following description. Detailed Implementation

[0026] The terminology used in this list takes into account the functionality of the invention and selects commonly used terms that are currently widely adopted. However, this may vary depending on the intent of those skilled in the art, precedents, the emergence of new technologies, etc. Furthermore, in some cases, there are terms arbitrarily chosen by the applicant; in such cases, their meanings will be described in detail in the description of the corresponding invention. Therefore, the terms used in this invention should not be mere names, but should be defined according to their meanings and the entirety of the invention.

[0027] Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Terms as defined in commonly used dictionaries should be interpreted as having the same meaning in the context of the relevant art and should not be construed as having an ideal or overly formal meaning unless explicitly defined in this application.

[0028] The numerical range includes the values ​​defined within the range stated. All maximum numerical limits given in this listing include all lower numerical limits, as explicitly stated. All minimum numerical limits given in this listing include all higher numerical limits, as explicitly stated. All numerical limits given in this listing will include all better numerical ranges within a larger numerical range, as explicitly stated.

[0029] The embodiments of the present invention are described in detail below, but it is self-evident that the present invention is not limited to the following embodiments.

[0030] According to the present invention, the functional cosmetic composition comprising stem cell culture medium is based on human adipose stem cells, comprising 100 parts by weight of stem cell culture medium selected from a group consisting of bone marrow, umbilical cord blood, peripheral blood, and adipose tissue basal layer, 30 to 40 parts by weight of purified water, 1 to 5 parts by weight of prickly ash, 3 to 7 parts by weight of Trehalose, 5 to 20 parts by weight of serine, 1 to 5 parts by weight of gossypol ester, and 1 to 5 parts by weight of sulfur urea. It also contains 0.3 to 1 part by weight of essential oil and 0.1 to 0.5 parts by weight of other essential oils.

[0031] The stem cell culture medium consists of peptides and proteins, which may include PDGF (deformation growth factor), Basic FGF (basic fibroblast growth factor), KGF (keratinocyte growth factor), TGF-β1 (deformation growth factor), HGF (hepatocyte growth factor), VEGF (vascular endothelial growth factor), collagen, pentazocine, and SOD (superoxide dismutase).

[0032] Ham seed oil may include CBD (cannabidiol) and cypress extract extracted from ham root or stem, 0.05 to 0.1 part by weight of ethanol, and 0.10 to 0.1 part by weight of arginine.

[0033] The extraction of cypress extract can be divided into several stages: pulverizing cypress, washing the pulverized cypress in water at 10 to 20 degrees Celsius, heating the washed cypress in water at 50 degrees Celsius, removing the heated cypress from the water, sealing the cypress removed from the water in a plastic bucket or plastic bucket to form moisture, reheating the cypress that has formed moisture, mixing the heated cypress with heated cypress water, mixing the cypress with heated water, and mixing the cypress with heated water.

[0034] At this time, there may be sores on the cypress tree, so it is best to seal it in a plastic bucket.

[0035] At this point, the plastic bucket can be modified into a regular plastic bucket. Although the plastic bucket itself isn't urbanized, a containment board for holding cypress can be placed inside. The containment board might be made of metal to prevent the cypress from absorbing moisture. Furthermore, a cloth can be prepared at the top of the containment board to wrap the cypress. This is because the moisture from the cypress is absorbed by the cloth, allowing extracts of the cypress to be squeezed out. Additionally, elastic components can be inserted inside the metal. These elastic components can absorb external impacts from the outside of the plastic bucket, preventing the cypress held on the containment board from falling off due to such impacts.

[0036] The essential oil is likely to be at least one of lavender, tea tree, geranium, and majolica.

[0037] Functional cosmetic compositions, including those using stem cell culture medium, can incorporate a composite solution of extracts of peppermint and oregano in a 1:1 weight ratio. In this case, the peppermint and oregano may be present in quantities of 0.5 to 2 parts by weight.

[0038] Next, by using stem cell culture medium with added compound solution, more pumpkin seed powder can be added to the manufactured cosmetic composition.

[0039] At this stage, the production of pumpkin seed powder can be divided into several steps: washing the pumpkin seeds, drying the washed pumpkin seeds on a dry towel for 5 hours, soaking the dried pumpkin seeds in Nipah coconut flower stem extract for 16 to 24 hours, drying the pumpkin seeds soaked in Nipah coconut flower stem extract at room temperature for 7 to 10 hours, and pulverizing the dried pumpkin seeds to a particle size of 15 to 30 μm. The pumpkin seed powder can be produced in a concentration of 0.5 to 1 g. Alternatively, pumpkin seed powder can be replaced by pumpkin seed extract.

[0040] The Nipah palm flower stem extract can be prepared into an extract obtained by hot water extraction and concentration using cut Nipah palm flower stems as an aqueous solvent.

[0041] Furthermore, the extract of Nipah coconut flower stems may be 5 to 10 grams.

[0042] In addition, Nipah coconut flower stem extract is characterized by containing Nipah coconut flower stem extract as its active ingredient.

[0043] At this point, the extract of Nipah palm flower stem refers to hot water extraction or ultrasonic extraction using Nipah palm flower stem as a solvent.

[0044] In addition, the powder from the flower stalks of the Nipah coconut can also be used as an active ingredient.

[0045] Among them, the Nipah palm (NIPA FRUTICANS WURMB) is native to Southeast Asia, including India and Malaysia, and Australia, growing in wetlands such as mangrove forests. Its roots split into multiple segments underground, and its leaves cluster above ground, displaying a glossy green color. Furthermore, it produces both male and female flowers per plant, which emerge from the leaf axils above ground, typically on flower stalks between October and June of the following year.

[0046] The flower stems of these Nipa coconuts are collected and extracted for use. The reason for using the flower stems in particular is that, compared to any other part of the Nipa coconut, the flower stems contain a large amount of antioxidants such as polyphenols and selenium, as well as various nutrients.

[0047] The compositional analysis and detection results of Nipah Palm are shown in Tables 1 and 2. The flower stems of Nipah Palm not only contain polyphenols, flavonoids, saponins, etc., but also contain a large amount of aspartic acid, theaflavins, histidine and other effective components.

[0048] Table 1

[0049] Component analysis results of Nipah coconut flower stem

[0050] Inspection items unit Inspection results Inspection items unit Inspection results Moisture ％ 20.54 selenium μg / kg 154.57 histones ％ 11.65 manganese mg / kg 82.58 Crude fat ％ 0.73 Salt mg / kg 18344.65 Click count ％ 7.95 sulfur mg / kg 3221.83 carbohydrate ％ 59.15 sodium mg / kg 6832.66 Calories kcal / 100g 289.77 saponins mg / kg 110161.07 calcium mg / kg 3009.62 Total polyphenols Brix 173443.90 phosphorus mg / kg 2589.65 Total flavonoids mg / kg 616.24 iron mg / kg 119.57 Total sugar ％ 11.84 Potassium mg / kg 26211.57 fructose ％ 1.57 magnesium mg / kg 4670.47 glucose ％ 1.11 copper mg / kg 0.26 sucrose ％ 1.19 Zinc mg / kg 14.13

[0051] Table 2

[0052] Component analysis results of Nipah coconut flower stems

[0053] Inspection items unit Inspection results Aspartame mg / kg 269.01 Treonin mg / kg 30.22 Selin mg / kg 30.59 glutamic acid mg / kg 171.29 proline mg / kg 0 glycine mg / kg 7.98 Methionine mg / kg 25.69 Bahrain mg / kg 49.06 Isolusin mg / kg 39.30 Lu Xin mg / kg 49.73 Tyrosine mg / kg 0 Benmaraning mg / kg 14.70 Histidine mg / kg 13.44 Lysine mg / kg 16.26 Arginine mg / kg 302.53 Sistine mg / kg 30.19 Methionine mg / kg 32.46 Tryptophan mg / kg 0

[0054] Referring to the table above, extracts from the stems of the Nipah palm can provide antioxidant and antibacterial benefits, helping to moisturize and keep the skin clean. Additionally, its anti-inflammatory properties can alleviate skin inflammation. Furthermore, adding Nipah palm stem powder can help exfoliate and keep the skin clean.

[0055] In addition, the functional cosmetic composition containing the stem cell culture medium of the present invention may include, as an active ingredient, 0.1 to 5 parts by weight of seaweed extract.

[0056] Furthermore, Asparagopsis taxiformis is a type of red algae distributed worldwide in tropical and temperate seas. Extracts from this algae possess excellent antibacterial activity, alleviating various problems and improving skin tone. Additionally, when used with stem cell culture medium, it exhibits even better effects in improving wrinkles and elasticity.

[0057] In addition, sea jasmine extract can be extracted from sea jasmine using one or more solvents, including water, ethanol, and methanol, using conventional methods.

[0058] Moreover, the functional cosmetic composition containing the stem cell culture medium of the present invention may further include 0.1 to 5 ginkgo leaf extract as an active ingredient.

[0059] Cosmetics containing ginkgo leaf extract can have a soothing effect on the skin. This is because ginkgo leaves are rich in antioxidants, which can reduce inflammation. This can alleviate skin problems or inflammatory conditions.

[0060] The foregoing description of the present invention is for illustrative purposes only. Those skilled in the art will understand that it can be readily modified into other specific forms without altering the technical concept or essential features of the invention. Therefore, the embodiments described above must be understood as exemplary in all aspects, not limiting. For example, each component described in a single form can be implemented separately; similarly, implementations in a combined form are described as separate components.

[0061] The scope of this invention is defined by the following scope of claims, which should be interpreted as including the meaning and scope of the scope of claims and all variations or modifications derived therefrom.

Claims

1. A functional cosmetic composition, wherein, The functional cosmetic composition comprises, based on 100 parts by weight of stem cell culture medium selected from a group consisting of human adipose stem cells, bone marrow, umbilical cord blood, peripheral blood, and adipose tissue basal layer, 30 to 40 parts by weight of purified water, 1 to 5 parts by weight of plowshare, 3 to 7 parts by weight of Trehalose, 5 to 20 parts by weight of serine, 1 to 3 parts by weight of depantenol, 1 to 5 parts by weight of organic sulfur, 1 to 5 parts by weight of liquid methionine, 0.3 to 1 part by weight of hamp seed oil, and 0.1 to 0.5 parts by weight of aromatic oil. The stem cell culture medium contains peptides and proteins, including PDGF (deformation growth factor), Basic FGF (basic fibroblast growth factor), KGF (keratinocyte growth factor), TGF-β1 (deformation growth factor), HGF (hepatocyte growth factor), VEGF (vascular endothelial growth factor), collagen, five thrombins, and antioxidant enzymes. The Hamp seed oil comprises CBD (cannabidiol) and cypress extract extracted from the roots or stems of ham, the raw material for ham extraction, as well as 0.05 to 0.1% by weight of ethanol and 0.10 to 0.1% by weight of arginine. The cypress extract is prepared by the following steps: Crushed cypress; Wash the crushed cypress in water at 10 to 20 degrees Celsius; The cypress leaves to be cleaned are heated in water at 50 degrees Celsius; Remove the heated cypress from the water; The cypress removed from the water is sealed in plastic or a plastic bucket to create moisture. Reheat the cypress leaves that have become damp; Mix heated cypress water with reheated cypress water; and Distilled mixed water, The essential oil is at least one of lavender, tea tree, geranium, and majolica.

2. The functional cosmetic composition according to claim 1, wherein, In a cosmetic composition prepared using the aforementioned stem cell culture medium, a composite solution of extracts from peppermint and oregano mixed in a 1:1 weight ratio is added.

3. The functional cosmetic composition according to claim 2, wherein, Pumpkin seed powder is added to a cosmetic composition prepared using the stem cell culture medium containing the compound solution.

4. The functional cosmetic composition according to claim 3, wherein, The pumpkin seed powder is manufactured through the following steps: Clean the pumpkin seeds; Place the washed pumpkin seeds on a dry towel to dry for 5 hours; Soak dried pumpkin seeds in Nipah coconut flower stem extract for 16 to 24 hours; Pumpkin seeds soaked in Nipah coconut flower stem extract were dried at room temperature for 7 to 10 hours; and Crush the dried pumpkin seeds into 15 to 30 μm particles.

5. The functional cosmetic composition according to claim 4, wherein, The Nipah coconut flower stem extract is manufactured through the following steps: The cut Nipa palm flower stems were extracted using water as a solvent via hot water extraction; and The extracted extract is concentrated.