Packaging cell line for efficient propagation of third generation rabies virus and its preparation and use

CN122104595APending Publication Date: 2026-05-29LINGANG LAB
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LINGANG LAB
Filing Date
2024-11-27
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

The lack of cell lines for the efficient production of third-generation rabies virus (RVΔL) in current technologies limits its application in neuroscience research, especially due to its high toxicity and low titer.

Method used

An engineered BHK cell line was constructed, whose genome integrates a foreign expression cassette that co-expresses B19L and a reporter gene. Cells that efficiently proliferate third-generation rabies virus were obtained by flow cytometry sorting. The specific steps included vector transfection and fluorescence intensity screening to form the BHK-B19L-EGFP-1-2 cell line.

Benefits of technology

It has achieved efficient production of low-toxicity third-generation rabies virus with a stable viral titer above 1E6 iu/mL, suitable for neuronal inverse labeling and calcium imaging studies, and has efficient proliferation and stable viral production capabilities.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present application relates to a packaging cell line for efficiently propagating third-generation rabies virus and its preparation method and application. Specifically, the present application provides an engineered cell line for efficiently propagating third-generation rabies virus, wherein an exogenous expression cassette expressing B19L and a fluorescent reporter gene is integrated into the genome of the cell, which can be used for packaging and producing third-generation rabies virus with L protein deletion. The cell line of the present application can be used for efficiently propagating third-generation rabies virus, and the obtained third-generation rabies virus has low toxicity, high titer and persistent neuron labeling ability, and is particularly suitable for neuron-related functional studies.
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