A processing method of a conchoidal sea cucumber small peptide and application thereof in organic water-soluble fertilizer
By using compound enzymatic hydrolysis and membrane separation technology, the cooking liquid of sea centipede is converted into highly active small peptides, which are then combined with potassium humate and seaweed extract to construct a synergistic network. This solves the problems of poor component synergy and insufficient flavor regulation in water-soluble fertilizers, and achieves simultaneous improvement in crop yield and flavor quality.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- LIAONING YURUN AGRI DEV CO LTD
- Filing Date
- 2026-04-28
- Publication Date
- 2026-05-29
AI Technical Summary
Existing water-soluble fertilizers suffer from poor component synergy, insufficient small peptide activity, and lack of flavor regulation effects, making it difficult to meet the comprehensive needs of promoting growth, increasing yield, and improving quality. Furthermore, the by-products of sea centipede processing are not fully utilized.
A composite enzymatic hydrolysis technique was used to convert the cooking liquid of sea centipede into highly active small peptides with molecular weights of 3kDa-10kDa. These peptides were then combined with potassium humate and seaweed extract to construct a signal-carrier-nutrient synergistic network, which activated plant physiological responses and optimized crop growth and flavor compound synthesis.
It has enabled the high-value utilization of waste liquid from the processing of sea centipedes, increased crop yield and the content of flavor substances in fruits, promoted the healthy growth of crops and the systematic optimization of flavor quality, and achieved an efficiency improvement of 30%-60%.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of agricultural fertilizer technology, and in particular to a method for processing small peptides from sea centipedes and their application in organic water-soluble fertilizers. Background Technology
[0002] With the development of green agriculture, organic water-soluble fertilizers have become an important alternative to traditional chemical fertilizers due to their advantages such as easy nutrient absorption and environmental friendliness. Currently, water-soluble fertilizers often use humic acid, seaweed extracts, and animal and plant-derived peptides as core components, and achieve the dual functions of nutrient supply and crop growth regulation through compound formulation.
[0003] Aquatic product processing byproducts are rich in high-quality protein, making them ideal raw materials for preparing bioactive small peptides. However, the cooking liquid from sea centipede processing is often discarded, resulting in resource waste and environmental pressure. Current technologies have not fully explored its application value in water-soluble fertilizers. Meanwhile, conventional water-soluble fertilizers suffer from poor component synergy, insufficient small peptide activity, and a lack of flavor control effects, often relying on single nutrient supplies and failing to meet the comprehensive needs of "promoting growth, increasing yield, and improving quality."
[0004] Furthermore, the preparation of small peptides often employs a single enzymatic hydrolysis process, resulting in uneven molecular weight distribution of the products. Moreover, the existing compound systems lack a clear synergistic mechanism among the components, leading to unstable fertilizer efficacy. Therefore, developing an organic water-soluble fertilizer that uses sea centipede byproducts as raw materials, employs compound enzymatic hydrolysis to prepare active small peptides, and exhibits synergistic effects among its components is of great significance for resource utilization and improving agricultural quality and efficiency. Summary of the Invention
[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a method for processing small peptides from sea centipedes and their application in organic water-soluble fertilizers.
[0006] To achieve the above objectives, the present invention adopts the following technical solution: This invention first proposes a method for processing small peptides from sea centipedes, comprising the following steps: S1. Raw material pretreatment: Collect the cooking liquid produced by processing sea centipedes, centrifuge or filter to remove solid impurities, and obtain a clear protein solution; S2. Complex enzymatic hydrolysis: Adjust the pH of the clarified protein solution to 6.0-7.0 and the temperature to 45℃-55℃, then add a complex protease to carry out the enzymatic hydrolysis reaction for 2h-6h to obtain the enzymatic hydrolysate; The complex protease is composed of flavor protease and animal protease in a mass ratio of 1.5-2.5:1, with a total addition of 500U-800U per gram of raw protein. Single proteases have limited cleavage sites. By combining flavor proteases with animal proteases, a more comprehensive and in-depth "pruning" of the centipede protein can be achieved, resulting in the enrichment of specific amino acid sequences and peptides with molecular weights primarily between 3kDa and 10kDa. Peptides in this molecular weight range possess both good membrane permeability and biological activity. Flavor proteases can expose terminal umami amino acids, such as glutamic acid, while animal proteases produce more core peptide sequences with physiological regulatory functions.
[0007] S3. Inactivation and separation: Heat the enzyme hydrolysate to 80℃-90℃ and maintain for 10min-20min to inactivate the enzyme, then perform solid-liquid separation to obtain the clear peptide hydrolysate; S4. Membrane filtration purification: The small peptide enzymatic hydrolysate is filtered sequentially through a microfiltration membrane and an ultrafiltration membrane, and the permeate is collected; The microfiltration membrane has a pore size of 0.05 μm-0.2 μm, and the ultrafiltration membrane has a molecular weight cutoff of 3 kDa-10 kDa. S5. Vacuum concentration: The purified permeate is concentrated at low temperature under vacuum at 50℃-65℃ and a vacuum degree of -0.07MPa to -0.09MPa to obtain a concentrated solution of sea centipede small peptides.
[0008] Preferably, the concentrate contains ≥60% small molecule peptides with a molecular weight of 3kDa-10kDa.
[0009] The present invention also provides an organic water-soluble fertilizer, comprising the following components in parts by weight: Concentrated small peptides from centipede (on a dry basis): 2-8 parts; Potassium humate: 5-10 parts; Seaweed extract powder: 1-3 parts; Nitrogen source, phosphorus source and potassium source: 10-20 parts; Micronutrients: 2-5 parts; By adding the above components and diluting with agricultural water, an organic water-soluble fertilizer with a total solids content of 20%-35% is obtained.
[0010] The compound system constructs a four-in-one synergistic network of "signal-carrier-nutrient-microecology" with sea centipede small peptides as the core: Enriched 3kDa-10kDa small peptides can act as ligand analogs, binding to receptors on the plant cell membrane surface, such as receptor kinases (RLKs), activating signaling pathways such as MAPK; initiating the expression of downstream defense response genes (such as PR proteins) and quality metabolism-related genes (such as sugar transporters and volatile ester synthase genes); at the same time, the defense / growth signals activated by the small peptides interact with the pathways regulated by endogenous hormone analogs in seaweed extracts, such as auxin, cytokinin, and betaine, synergistically optimizing the allocation of "growth-defense-metabolism" resources in crops, enabling plants to direct more resources toward the synthesis and accumulation of fruit flavor substances (sugars, acids, and aromas) while growing healthily.
[0011] Humic acid macromolecules possess abundant functional groups such as carboxyl and phenolic hydroxyl groups, which can spontaneously bind with small peptides from sea centipedes through hydrogen bonds, ionic bonds, and hydrophobic interactions to form a "humic acid-small peptide" organic complex. This complex slows down the rapid degradation of small peptides by microorganisms in the soil, achieving a sustained release of active signals. Simultaneously, it protects the small peptides from immediate fixation by soil colloids, improving their mobility and bioavailability in the rhizosphere. Potassium humate itself can improve soil structure, and the potassium ions it carries also aid in the transport and enlargement of fruit sugars, creating a favorable physicochemical microenvironment for the small peptides and nutrients to function effectively.
[0012] Centipede peptides and humic acid are high-quality, easily metabolized organic carbon and nitrogen sources, but their molecular structures are selective for microorganisms. They preferentially stimulate the proliferation of beneficial microorganisms such as phosphate-solubilizing bacteria, nitrogen-fixing bacteria, and biocontrol bacteria, while inhibiting soil-borne pathogens, thereby creating a healthier and more active rhizosphere microbiome. The metabolic activities of these beneficial microorganisms further activate soil nutrients and produce secondary metabolites such as vitamins, amino acids, and plant hormones, which complement and synergize with the directly applied peptides and seaweed extracts, continuously promoting crop growth and health from the rhizosphere ecological level.
[0013] Trace elements (such as Zn, Fe, and Mn) in amino acid / sugar alcohol chelates are inherently highly stable. Sea centipede peptides and humic acid can also act as natural chelating agents, forming organic complexes with these metal ions to prevent their fixation in the soil and promoting their absorption through potential peptide transport channels.
[0014] Preferably, the nitrogen source is selected from one or two of urea and potassium nitrate; the phosphorus source is ammonium dihydrogen phosphate; and the potassium source is potassium dihydrogen phosphate, and the three are prepared in a mass ratio of 1:1:1.
[0015] Preferably, the trace elements are at least three of the elements selected from calcium, magnesium, iron, zinc, boron, manganese, and copper, and are added in the form of amino acid chelates, sugar alcohol chelates, or EDTA chelates.
[0016] Preferably, the organic water-soluble fertilizer has a pH value of 5.5-6.5, and the total content of endogenous hormone analogs in the seaweed extract powder is ≥0.5%.
[0017] This invention also proposes a method for preparing the aforementioned organic water-soluble fertilizer, comprising the following steps: A. Dissolve potassium humate, seaweed extract powder, nitrogen source, phosphorus source, potassium source and trace elements in a portion of water, and stir until completely dissolved to form a base solution; B. Under the stirring conditions of 25℃-35℃ and 150r / min-250r / min, slowly add the concentrated sea centipede peptide solution to the base solution and continue stirring for 30min-60min until the mixture is uniform. C. Add the remaining water and adjust the pH of the mixture to 5.5-6.5; D. After homogenization by a homogenizer, impurities are removed by filtration to obtain organic water-soluble fertilizer.
[0018] Preferably, the concentrated sea centipede peptide solution serves as a functional peptide source and signaling molecule, synergistically regulating crop growth and rhizosphere microecological regulation with humic acid and seaweed extract.
[0019] This invention also proposes that the aforementioned organic water-soluble fertilizer, when used in the cultivation of fruit and vegetable crops, can increase the content of flavor substances and yield of fruits and vegetables.
[0020] Compared with the prior art, the beneficial effects of the present invention are: 1. Existing technologies often simply treat or discard the cooking liquid of sea centipedes. This invention innovatively utilizes "flavor / animal compound enzyme-directed enzymatic hydrolysis" combined with "microfiltration-ultrafiltration membrane precise fractionation" technology to convert the proteins into highly active small peptides with molecular weights of 3kDa-10kDa and enrich them to over 60%. This invention not only transforms waste liquid into core raw materials with clear biostimulatory functions, solving environmental and cost issues, but also ensures the stability and controllability of product activity through standardized preparation, laying a material foundation for subsequent in-depth applications that surpasses traditional crude extracts.
[0021] 2. Traditional organic water-soluble fertilizers are mostly simple mixtures of materials with weak synergistic effects. This invention scientifically combines small peptides, potassium humate, and seaweed extract, revealing a three-level synergistic mechanism of "signal-carrier-nutrient": small peptides act as initiating signals to activate plant physiological responses; humic acid acts as an intelligent carrier to achieve slow release of small peptides and root environment improvement; and the active substances in seaweed cross-enhance stress resistance and growth pathways. The three work synergistically to construct a synergistic network of "1+1+1>3," resulting in a 30%-60% increase in key indicators such as growth promotion and stress resistance in experiments, achieving a qualitative leap from "nutrient supplementation" to "system regulation."
[0022] 3. Existing fertilizers struggle to balance yield and flavor quality. This invention utilizes small peptides to precisely regulate secondary metabolism, synergistically converting photosynthetic products into flavor compounds such as sugars, acids, and esters through the efficient and targeted transformation of photosynthetic products. Field trials have demonstrated that this product, while ensuring increased yield, can enhance the total content of volatile aroma compounds in fruits like strawberries by over 45%, and significantly optimize the sugar-acid ratio.
[0023] In summary, this invention achieves high-value and standardized utilization of wastewater from the processing of sea centipedes. Through compound enzymatic hydrolysis and membrane separation technology, it transforms the wastewater into a core raw material rich in specific active peptides. The developed organic water-soluble fertilizer innovatively constructs a synergistic network with this peptide as the signaling center, working in conjunction with humic acid, seaweed extract, and nutrients, overcoming the limitations of simple mixing in traditional fertilizers. The final product, while increasing crop yield, systematically optimizes the sugar-acid ratio and volatile aroma content of fruits, providing an effective technical solution to address the bottleneck of "increased yield without improved quality" in agriculture. Detailed Implementation
[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with existing known technologies. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.
[0025] Example 1: An organic water-soluble fertilizer, comprising the following components by weight: Concentrated small peptide solution of centipede on a dry basis: 2 kg; Potassium humate: 10 kg; Seaweed extract powder: 1kg; Nitrogen, phosphorus, and potassium sources: 20 kg; 0.1 kg of EDTA-Ca, 0.1 kg of EDTA-Mg, 0.03 kg of EDTA-Fe, 0.04 kg of EDTA-Zn, 0.01 kg of sodium octaborate tetrahydrate, 0.02 kg of EDTA-Mn, and 0.005 kg of EDTA-Cu; Total solids content: 35%.
[0026] The nitrogen source is urea; the phosphorus source is ammonium dihydrogen phosphate; and the potassium source is potassium dihydrogen phosphate, which are mixed in a mass ratio of 1:1:1.
[0027] The preparation method of organic water-soluble fertilizer includes the following steps: A. Dissolve potassium humate, seaweed extract powder, nitrogen source, phosphorus source, potassium source and trace elements in 50L of water and stir until completely dissolved to form a base solution; B. Under stirring conditions of 30℃ and 200r / min, slowly add the concentrated sea centipede peptide solution to the base solution and continue stirring for 45min until the mixture is uniform. C. Add the remaining water and adjust the pH of the mixture to 6.0; D. After homogenization by a homogenizer, impurities are removed by filtration to obtain organic water-soluble fertilizer.
[0028] The processing method for sea centipede small peptides includes the following steps: S1. Raw material pretreatment: Collect the cooking liquid produced by processing sea centipedes, centrifuge or filter to remove solid impurities, and obtain a clear protein solution; S2. Complex enzymatic hydrolysis: Adjust the pH of the clarified protein solution to 6.0 and the temperature to 50℃, then add a complex protease and carry out the enzymatic hydrolysis reaction for 4 hours to obtain the enzymatic hydrolysate; The complex protease is composed of flavor protease and animal protease in a mass ratio of 1.5:1, with a total addition of 800U per gram of raw protein. S3. Inactivation and separation: The enzyme hydrolysate is heated to 80℃ and maintained for 20 min to inactivate the enzyme, and then solid-liquid separation is performed to obtain the clear peptide hydrolysate. S4. Membrane filtration purification: The small peptide enzymatic hydrolysate is filtered sequentially through a microfiltration membrane and an ultrafiltration membrane, and the permeate is collected; The microfiltration membrane has a pore size of 0.2 μm, and the ultrafiltration membrane has a molecular weight cutoff of 3 kDa-10 kDa. S5. Vacuum concentration: The purified permeate is concentrated at 60°C and a vacuum degree of -0.08MPa to obtain a concentrated solution of centipede small peptides.
[0029] The concentrate contains ≥60% small molecule peptides with a molecular weight of 3kDa-10kDa.
[0030] Example 2: An organic water-soluble fertilizer, comprising the following components by weight: Concentrated small peptide solution of centipede on a dry basis: 5 kg; Potassium humate: 7.5 kg; Seaweed extract powder: 2kg; Nitrogen, phosphorus, and potassium sources: 15 kg; 0.15 kg of EDTA-Ca, 0.075 kg of EDTA-Mg, 0.045 kg of EDTA-Fe, 0.03 kg of EDTA-Zn, 0.015 kg of sodium octaborate tetrahydrate, 0.015 kg of EDTA-Mn, and 0.0075 kg of EDTA-Cu; Total solids content: 28%.
[0031] The nitrogen source is potassium nitrate; the phosphorus source is ammonium dihydrogen phosphate; and the potassium source is potassium dihydrogen phosphate, which are mixed in a mass ratio of 1:1:1.
[0032] The preparation method of organic water-soluble fertilizer includes the following steps: A. Dissolve potassium humate, seaweed extract powder, nitrogen source, phosphorus source, potassium source and trace elements in 50L of water and stir until completely dissolved to form a base solution; B. Under stirring conditions of 30℃ and 200r / min, slowly add the concentrated sea centipede peptide solution to the base solution and continue stirring for 45min until the mixture is uniform. C. Add the remaining water and adjust the pH of the mixture to 6.0; D. After homogenization by a homogenizer, impurities are removed by filtration to obtain organic water-soluble fertilizer.
[0033] The processing method for sea centipede small peptides includes the following steps: S1. Raw material pretreatment: Collect the cooking liquid produced by processing sea centipedes, centrifuge or filter to remove solid impurities, and obtain a clear protein solution; S2. Complex enzymatic hydrolysis: Adjust the pH of the clarified protein solution to 6.0 and the temperature to 50℃, then add a complex protease and carry out the enzymatic hydrolysis reaction for 4 hours to obtain the enzymatic hydrolysate; The complex protease is composed of flavor protease and animal protease in a mass ratio of 2:1, with a total addition of 600U per gram of raw protein. S3. Inactivation and separation: The enzyme hydrolysate is heated to 80℃ and maintained for 20 min to inactivate the enzyme, and then solid-liquid separation is performed to obtain the clear peptide hydrolysate. S4. Membrane filtration purification: The small peptide enzymatic hydrolysate is filtered sequentially through a microfiltration membrane and an ultrafiltration membrane, and the permeate is collected; The microfiltration membrane has a pore size of 0.2 μm, and the ultrafiltration membrane has a molecular weight cutoff of 3 kDa-10 kDa. S5. Vacuum concentration: The purified permeate is concentrated at 60°C and a vacuum degree of -0.08MPa to obtain a concentrated solution of centipede small peptides.
[0034] The concentrate contains ≥60% small molecule peptides with a molecular weight of 3kDa-10kDa.
[0035] Example 3: An organic water-soluble fertilizer, comprising the following components by weight: Concentrated sea centipede small peptide solution (dry basis): 8 kg; Potassium humate: 5 kg; Seaweed extract powder: 3kg; Nitrogen, phosphorus, and potassium sources: 10 kg; 0.2 kg of EDTA-Ca, 0.05 kg of EDTA-Mg, 0.06 kg of EDTA-Fe, 0.02 kg of EDTA-Zn, 0.02 kg of sodium octaborate tetrahydrate, 0.01 kg of EDTA-Mn, and 0.01 kg of EDTA-Cu; Total solids content: 20%.
[0036] The nitrogen source is urea; the phosphorus source is ammonium dihydrogen phosphate; and the potassium source is potassium dihydrogen phosphate, which are mixed in a mass ratio of 1:1:1.
[0037] The preparation method of organic water-soluble fertilizer includes the following steps: A. Dissolve potassium humate, seaweed extract powder, nitrogen source, phosphorus source, potassium source and trace elements in 50L, stir until completely dissolved to form the base solution; B. Under stirring conditions of 30℃ and 200r / min, slowly add the concentrated sea centipede peptide solution to the base solution and continue stirring for 45min until the mixture is uniform. C. Add the remaining water and adjust the pH of the mixture to 6.0; D. After homogenization by a homogenizer, impurities are removed by filtration to obtain organic water-soluble fertilizer.
[0038] The processing method for sea centipede small peptides includes the following steps: S1. Raw material pretreatment: Collect the cooking liquid produced by processing sea centipedes, centrifuge or filter to remove solid impurities, and obtain a clear protein solution; S2. Complex enzymatic hydrolysis: Adjust the pH of the clarified protein solution to 6.0 and the temperature to 50℃, then add a complex protease and carry out the enzymatic hydrolysis reaction for 4 hours to obtain the enzymatic hydrolysate; The complex protease is composed of flavor protease and animal protease in a mass ratio of 2.5:1, with a total addition of 500U per gram of raw protein. S3. Inactivation and separation: The enzyme hydrolysate is heated to 80℃ and maintained for 20 min to inactivate the enzyme, and then solid-liquid separation is performed to obtain the clear peptide hydrolysate. S4. Membrane filtration purification: The small peptide enzymatic hydrolysate is filtered sequentially through a microfiltration membrane and an ultrafiltration membrane, and the permeate is collected; The microfiltration membrane has a pore size of 0.2 μm, and the ultrafiltration membrane has a molecular weight cutoff of 3 kDa-10 kDa. S5. Vacuum concentration: The purified permeate is concentrated at 60°C and a vacuum degree of -0.08MPa to obtain a concentrated solution of centipede small peptides.
[0039] The concentrate contains ≥60% small molecule peptides with a molecular weight of 3kDa-10kDa.
[0040] The following comparison model was also set: Comparative Example 1: Based on Example 2, the difference is that the concentrated sea centipede peptide solution was removed and its dry basis ratio was replaced with an equal mass of potassium humate, while the rest was the same as in Example 2.
[0041] Comparative Example 2: Based on Example 2, the difference is that the centipede peptide concentrate was replaced with a centipede peptide concentrate with the same dry basis content prepared by enzymatic hydrolysis using only flavor protease (addition amount 600U / g raw material protein), and the rest was the same as in Example 2.
[0042] Comparative Example 3: Based on Example 2, the difference is that the seaweed extract powder is removed and replaced with an equal mass of water, while the rest is the same as in Example 2.
[0043] Comparative Example 4: Based on Example 2, the difference is that the sea centipede peptide concentrate was replaced with commercially available ordinary fish peptide concentrate with an equal dry basis content, and the rest is the same as Example 2.
[0044] Comparative Example 5: Water was used as a blank control.
[0045] Performance testing: A pot experiment was conducted. Tomato varieties with uniform growth were selected as the test crop and sown in seedling trays of uniform size. When the seedlings grew to 4 leaves and 1 heart, healthy and uniform seedlings were selected and transplanted into plastic pots (25cm in diameter and 20cm in height) containing an equal amount of mixed substrate nutrient soil, with 1 seedling per pot.
[0046] Each experimental group was repeated 10 times, for a total of 80 pots. During the cultivation period, the temperature was controlled at 25℃-28℃ during the day and 15℃-18℃ at night, and the relative humidity was maintained at 60%-70%. Natural light was supplemented with artificial light to maintain a photocycle of 12h light / 12h darkness.
[0047] After the seedlings have recovered from transplanting, fertilization treatment begins. Each fertilizer sample is diluted 1000 times with deionized water and then slowly poured along the pot wall. 200 mL is applied to each pot each time. The blank control group is treated with an equal amount of water. Fertilization is carried out 3 times during the entire growth period, with an interval of 15 days between each application.
[0048] Sixty days after the first fertilization, the height of the plant from the substrate surface to the growing point was measured using a measuring tape. The stem diameter at the third node above the base of the plant was measured using a digital caliper. The relative chlorophyll content (SPAD value) of the fully expanded functional leaves at the top of the plant was measured using a portable chlorophyll meter. Each indicator was measured three times per plant, and the average value was taken. After the fruit entered the ripening stage, the number of fruits with a diameter greater than 2 cm on each plant was counted as the fruit set per plant. All ripe fruits were harvested, and the weight of each fruit was measured using a 0.1g precision electronic balance to calculate the total yield per plant.
[0049] After harvesting, gas chromatography-mass spectrometry (GC-MS) was used. The fruits were placed in sealed bottles, and volatiles in the headspace were adsorbed through a fiber optic tube. The samples were then directly injected, and key aroma compounds such as aldehydes, alcohols, esters, and terpenes were identified through mass spectrometry library matching. After juicing, sucrose, fructose, and glucose (sweetness) were detected by high-performance liquid chromatography (HPLC); citric acid, malic acid, and tartaric acid (acidity) were detected. The sugar-acid ratio was calculated, and the results are shown below. Table 1. Statistics on plant height and yield of experimental groups
[0050] Table 2. Analysis of flavor compounds and sugar-acid ratio in the experimental group
[0051] Data Analysis: From the perspective of growth indicators, the overall performance of the example groups was significantly better than that of the comparative and blank controls. Among them, Example 2 (5% dry peptide, enzyme ratio 2:1) was the best, with a plant height of 86.3 cm, stem diameter of 10.5 mm, and chlorophyll SPAD value of 55.8, which were 47.3%, 61.5%, and 70.1% higher than the blank control, respectively, and 32.4%, 45.8%, and 44.9% higher than that of Comparative Example 1 (without small peptides). This indicates that the small peptides from *Scolopendra subspinipes* are the core of activating plant metabolic pathways and promoting nutrient absorption, and that the activity of small peptides prepared by the compound enzymatic hydrolysis process is better than that of single enzymatic hydrolysis (the indicators of Comparative Example 2 were 11.0%-20.6% lower than those of Example 2).
[0052] In terms of yield indicators, the total yield per plant in Example 2 was 3562.6g, an increase of 246.5% compared to the blank control, and an increase of 129.0%, 85.0%, and 47.5% compared to comparative examples 1, 3, and 4, respectively. The simultaneous increase in the number of fruits set and the weight of individual fruits confirms the implementation of the signal-nutrient coupling and rhizosphere microecological synergistic mechanism. The combination of small peptides with seaweed extract and humic acid can precisely regulate reproductive growth.
[0053] In terms of flavor quality, Example 2 showed a total aroma content of 428.9 μg / kg and a sugar-acid ratio of 9.84 (within the optimal flavor range), representing a 61.4% increase in aroma content and an 86.0% increase in sugar-acid ratio compared to Comparative Example 3 (without seaweed extract), demonstrating the synergistic effect of flavor precursor peptides and seaweed factors. After replacement with ordinary fish peptides (Comparative Example 4), the flavor indicators significantly decreased, proving the differentiated advantages of the sea centipede peptides.
[0054] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.
Claims
1. A method for processing small peptides from sea centipedes, characterized in that, Includes the following steps: S1. Raw material pretreatment: Collect the cooking liquid produced by processing sea centipedes, centrifuge or filter to remove solid impurities, and obtain a clear protein solution; S2. Complex enzymatic hydrolysis: Adjust the pH of the clarified protein solution to 6.0-7.0 and the temperature to 45℃-55℃, then add a complex protease to carry out the enzymatic hydrolysis reaction for 2h-6h to obtain the enzymatic hydrolysate; The complex protease is composed of flavor protease and animal protease in a mass ratio of 1.5-2.5:1, with a total addition of 500U-800U per gram of raw protein. S3. Inactivation and separation: Heat the enzyme hydrolysate to 80℃-90℃ and maintain for 10min-20min to inactivate the enzyme, then perform solid-liquid separation to obtain the clear peptide hydrolysate; S4. Membrane filtration purification: The small peptide enzymatic hydrolysate is filtered sequentially through a microfiltration membrane and an ultrafiltration membrane, and the permeate is collected; The microfiltration membrane has a pore size of 0.05 μm-0.2 μm, and the ultrafiltration membrane has a molecular weight cutoff of 3 kDa-10 kDa. S5. Vacuum concentration: The purified permeate is concentrated at low temperature under vacuum conditions of 5℃ (0-65℃) and vacuum degree of -0.07MPa to -0.09MPa to obtain a concentrated solution of sea centipede small peptides.
2. The method for processing sea centipede small peptides according to claim 1, characterized in that, The concentrate contains ≥60% small molecule peptides with a molecular weight of 3kDa-10kDa.
3. An organic water-soluble fertilizer, characterized in that, Includes the following components in parts by weight: Concentrated small peptides from centipede (on a dry basis): 2-8 parts; Potassium humate: 5-10 parts; Seaweed extract powder: 1-3 parts; Nitrogen source, phosphorus source and potassium source: 10-20 parts; Micronutrients: 2-5 parts; By adding the above components and diluting with agricultural water, an organic water-soluble fertilizer with a total solids content of 20%-35% is obtained.
4. The organic water-soluble fertilizer according to claim 3, characterized in that, The nitrogen source is selected from one or two of urea and potassium nitrate; the phosphorus source is ammonium dihydrogen phosphate; and the potassium source is potassium dihydrogen phosphate. The three are prepared in a mass ratio of 1:1:
1.
5. The organic water-soluble fertilizer according to claim 3, characterized in that, The aforementioned trace elements are at least three of the elements calcium, magnesium, iron, zinc, boron, manganese, and copper, and are added in the form of amino acid chelates, sugar alcohol chelates, or EDTA chelates.
6. The organic water-soluble fertilizer according to claim 3, characterized in that, The organic water-soluble fertilizer has a pH value of 5.5-6.5, and the total content of endogenous hormone analogs in the seaweed extract powder is ≥0.5%.
7. A method for preparing the organic water-soluble fertilizer according to any one of claims 3-6, characterized in that, Includes the following steps: A. Dissolve potassium humate, seaweed extract powder, nitrogen source, phosphorus source, potassium source and trace elements in a portion of water, and stir until completely dissolved to form a base solution; B. Under the stirring conditions of 25℃-35℃ and 150r / min-250r / min, slowly add the concentrated sea centipede peptide solution to the base solution and continue stirring for 30min-60min until the mixture is uniform. C. Add the remaining water and adjust the pH of the mixture to 5.5-6.5; D. After homogenization by a homogenizer, impurities are removed by filtration to obtain organic water-soluble fertilizer.
8. The application of the organic water-soluble fertilizer according to any one of claims 3-6, characterized in that, The concentrated sea centipede peptide solution, as a functional peptide source and signaling molecule, works synergistically with humic acid and seaweed extract to regulate crop growth and rhizosphere microecology.