A composition with uric acid-lowering effect, its preparation method and application

Abstract

This invention discloses a composition with uric acid-lowering effects, its preparation method, and its applications, relating to the field of biomedical technology. The composition comprises the following components in parts by weight: 30-50 parts of miracle fruit leaf extract, 10-20 parts of miracle fruit fruit extract, and 5-10 parts of miracle fruit seed ultrafine powder. By combining the miracle fruit leaf extract, miracle fruit extract, and miracle fruit seed ultrafine powder, a multi-target synergistic uric acid-lowering effect is achieved. This invention fully utilizes the resources of the entire miracle fruit plant, resulting in a natural, safe, and stable formula. It overcomes the shortcomings of traditional chemical drugs, such as significant side effects, limited natural product availability, and poor efficacy, making it suitable for long-term use and demonstrating significant advantages in the preparation of safe and effective uric acid-lowering drugs.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and in particular to a composition with uric acid-lowering effect, its preparation method and application. Background Technology

[0002] Hyperuricemia is a metabolic disease caused by disordered purine metabolism, leading to excessive uric acid production or reduced excretion. It is a significant contributing factor to various metabolic diseases, including gout, kidney damage, and cardiovascular disease. Currently used clinical drugs such as allopurinol and febuxostat are xanthine oxidase inhibitors. While their efficacy is definite, long-term use may cause side effects such as skin rashes and liver damage. Therefore, developing safe, effective, and long-term suitable natural uric acid-lowering products has significant market value.

[0003] Miracle fruit (Synsepalum dulcificum) is a tropical economic crop, known for the taste-altering glycoproteins in its pulp. However, current technologies mostly focus on the taste-altering properties of the pulp, neglecting the application value of its leaves and seeds in metabolic regulation. Recent studies have found that miracle fruit leaf extracts are rich in flavonoids (such as quercetin-3-O-α-L-rhamnoside and hyperoside), exhibiting strong xanthine oxidase inhibitory activity. The seeds have extremely high total phenolic content and strong antioxidant properties; the pulp is rich in anthocyanins and other pigments. However, current technologies have the following shortcomings: 1. Single utilization: Most methods only use a part of the miracle fruit (fruit or leaves), resulting in low efficacy; 2. Low extraction rate: Conventional alcohol or water extraction methods have limited dissolution of bound flavonoids and phenolic substances within the cell wall; 3. Unstable components: The active ingredients in miracle fruit (such as flavonoids and anthocyanins) are easily oxidized and degraded during extraction and storage, affecting efficacy. Summary of the Invention

[0004] The purpose of this invention is to provide a composition with uric acid-lowering effect, its preparation method, and its application, thereby solving the problems existing in the prior art. The composition provided by this invention has a synergistic uric acid-lowering effect and has significant advantages in the preparation of safe and efficient uric acid-lowering drugs.

[0005] To achieve the above objectives, the present invention provides the following solution: The present invention provides a composition having a uric acid-lowering effect, comprising the following components in parts by weight: 30-50 parts of miracle fruit leaf extract, 10-20 parts of miracle fruit fruit extract, and 5-10 parts of miracle fruit seed ultrafine powder.

[0006] Preferably, the composition comprises the following components in parts by weight: 40 parts of miracle fruit leaf extract, 15 parts of miracle fruit fruit extract, and 8 parts of miracle fruit seed ultrafine powder.

[0007] Furthermore, the composition further comprises the following components in parts by weight: 20-30 parts chicory root powder, 10-20 parts inulin, and 2-5 parts mogroside.

[0008] Preferably, the composition comprises the following components in parts by weight: 40 parts of miracle fruit leaf extract, 15 parts of miracle fruit fruit extract, 8 parts of miracle fruit seed ultrafine powder, 25 parts of chicory root powder, 15 parts of inulin, and 4 parts of mogroside.

[0009] Furthermore, the preparation method of the miracle fruit leaf extract or the miracle fruit fruit extract includes the following steps: After enzymatic hydrolysis of mirabilis leaves or fruits using a compound enzyme, the enzyme is inactivated to obtain the hydrolysate. Ethanol and ascorbic acid were added to the enzymatic hydrolysate, and the mixture was refluxed and extracted, then filtered to obtain the filtrate. The filtrate is dried to obtain the miracle fruit leaf extract or the miracle fruit fruit extract; The complex enzyme includes cellulase and pectinase.

[0010] Further, the mass ratio of the cellulase to the pectinase is 2:1; and / or The compound enzyme is present in a mass ratio of 0.6-2.5% relative to the leaves or fruit of the miracle fruit.

[0011] Furthermore, the ascorbic acid is present in a mass ratio of 0.1-0.5% relative to the miracle fruit leaf or miracle fruit.

[0012] The present invention also provides a method for preparing the above-mentioned composition, comprising the step of mixing the components uniformly to obtain the composition.

[0013] The present invention also provides the use of the above-described composition in the preparation of uric acid-lowering drugs.

[0014] The present invention also provides a uric acid-lowering drug, wherein the active ingredient comprises the above-described composition.

[0015] The present invention discloses the following technical effects: This invention provides a composition with uric acid-lowering effects, achieved through a combination of miracle fruit leaf extract, miracle fruit fruit extract, and miracle fruit seed ultrafine powder (which may be further supplemented with chicory root powder, inulin, and mogrosides), resulting in multi-target synergistic uric acid-lowering. Compared with single ingredients, the three-component combination index (CI) is 0.410, exhibiting a significant synergistic effect and an IC50 inhibitory effect on xanthine oxidase. 50 The uric acid level was reduced to 28.15 μg / mL, significantly improving the uric acid-lowering effect.

[0016] This invention employs a combined enzymatic hydrolysis and ascorbic acid-protected extraction process, which effectively improves the total flavonoid yield and activity retention rate compared to conventional alcohol extraction, and can effectively solve the problems of low extraction rate and easy oxidation and degradation of components.

[0017] Animal experiments showed that the composition could significantly reduce serum uric acid, liver xanthine oxidase activity and inflammatory factor IL-1β levels in hyperuricemic mice. The high-dose group had uric acid levels close to normal and no obvious liver and kidney damage.

[0018] This invention makes full use of the entire plant resources of Miracle Fruit, with a natural formula, high safety, and stable efficacy. It overcomes the shortcomings of traditional chemical drugs, such as large side effects, single natural product, and poor efficacy. It is suitable for long-term use and has significant advantages in the preparation of safe and effective uric acid-lowering drugs. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 A statistical chart showing the total flavonoid yield of miracle fruit leaf extract under different extraction process conditions. Detailed Implementation

[0021] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0022] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0023] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0024] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0025] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0026] Example 1 Preparation method of miracle fruit leaf extract: (1) Take dried miracle fruit leaves, crush them and pass them through a 40-mesh sieve to obtain miracle fruit leaf powder; (2) Take 1 kg of miracle fruit leaf powder, add 15 g of compound enzyme (the mass ratio of cellulase and pectinase is 2:1), add 8 L of citrate buffer, adjust the pH to 5.0, and enzymatically hydrolyze at 50℃ for 90 minutes; (3) After the enzymatic hydrolysis is completed, the temperature is raised to 80℃ to inactivate the enzyme for 15 minutes, then ethanol is added to make the final ethanol concentration reach 70% (v / v), 3g ascorbic acid is added, and the mixture is refluxed at 70℃ for 2 hours. The filtrate is then collected by filtration. (4) The filtrate was concentrated under reduced pressure at 50°C and spray-dried to obtain the miracle fruit leaf extract.

[0027] Experimental Example 1 The enzyme dosage in Example 1 was adjusted to 0, 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, 1.8%, 2.0%, and 2.5%, respectively, while other operations remained the same as in Example 1, resulting in different enzymatic hydrolysates. A conventional alcohol extraction method was used as a control. The conventional alcohol extraction method is as follows: (1) Take dried miracle fruit leaves, crush them and pass them through a 40-mesh sieve to obtain miracle fruit leaf powder; (2) Take 1 kg of miracle fruit leaf powder, add 12 L of 70% (v / v) ethanol aqueous solution, reflux extract at 80℃ for 2 hours, filter and collect the first filtrate. Add 12 L of 70% (v / v) ethanol aqueous solution to the filter residue and reflux extract once more, filter and collect the second filtrate; (3) Combine the two filtrates, concentrate under reduced pressure at 50°C, and spray dry to obtain miracle fruit leaf extract.

[0028] The total flavonoid yield of the enzymatic hydrolysate prepared by the compound enzyme-assisted extraction process under different enzyme dosage conditions was detected; at the same time, the total flavonoid yield of the total filtrate prepared by conventional alcohol extraction at different times was detected. The results are shown in Table 1. The results show that the addition of enzyme can significantly improve the total flavonoid yield; the enzyme dosage range of 0.6%-2.5% has a highly significant synergistic effect.

[0029] Table 1. Effect of compound enzyme dosage on total flavonoid yield Note: Data are expressed as mean ± standard deviation (n=3); the improvement rate compared to conventional alcohol extraction = (yield of this group - optimal yield of conventional alcohol extraction 3.15%) / 3.15% × 100%; significance was determined by one-way ANOVA followed by Dunnett's test, compared with the 0 enzyme dosage control group: P <0.05, P <0.01.

[0030] Experiment Example 2 The enzymatic hydrolysis time in Example 1 was adjusted to 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, and 300 min, respectively, while other operations remained the same as in Example 1, resulting in different enzymatic hydrolysates. A conventional alcohol extraction method (see Experimental Example 1) was used as a control, and the extraction time was adjusted to 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, and 300 min, respectively.

[0031] The total flavonoid yield of reflux extract filtrates prepared using a compound enzyme-assisted extraction process at different enzymatic hydrolysis times was determined; simultaneously, the total flavonoid yield of total filtrates prepared using conventional ethanol extraction at different times was also determined. The results are shown in Table 2 and [Table data missing]. Figure 1 The results showed that, compared with traditional conventional alcohol extraction, the compound enzyme-assisted extraction method could effectively improve the yield of total flavonoids.

[0032] Table 2. Total flavonoid yield (%) of miracle fruit leaves under different extraction processes Note: Data are expressed as mean ± standard deviation (n=3); This indicates that, compared with the conventional alcohol extraction group, the difference was extremely significant at the same time point. P<0.01), and statistical analysis was performed using an independent samples t-test; the difference in total flavonoid yield between the two extraction methods was statistically significant at all non-zero time points; in conventional alcohol extraction, extraction time refers to the total time of two reflux extractions; in compound enzyme-assisted extraction, extraction time refers to the enzymatic hydrolysis time + reflux extraction time.

[0033] Experimental Example 3 The ascorbic acid content in Example 1 was adjusted to 0, 0.1%, 0.2%, 0.4% and 0.5% respectively, and other operations were the same as in Example 1 to prepare different mystery fruit leaf extracts.

[0034] The total flavonoid yield of the enzymatic hydrolysate before reflux extraction and the filtrate after reflux extraction was measured, and the results are shown in Table 3. The results indicate that the addition of ascorbic acid can improve the retention rate of total flavonoids.

[0035] Table 3. Effect of ascorbic acid addition on the retention rate of total flavonoids in the extract. Note: Data are expressed as mean ± standard deviation (n=3); one-way ANOVA was used to compare the total flavonoid yield after 2 hours among the six groups and compared it with the initial total flavonoid yield: P <0.05, P <0.01, P <0.001.

[0036] Comparative Example 1 Miracle fruit leaf extract was prepared using the conventional alcohol extraction method described in Experiment Example 1.

[0037] Comparative Example 2 Same as Example 1, except that the complex enzyme is replaced with cellulase.

[0038] Comparative Example 3 Same as Example 1, except that the complex enzyme is replaced with pectinase.

[0039] The total flavonoid yields of Example 1 and Comparative Examples 1-3 were statistically analyzed, and the results are shown in Table 4. The results show that the method of the present invention can effectively improve the total flavonoid yield.

[0040] Table 4 Comparison of optimal conditions and total flavonoid yield for different extraction processes Note: Data are expressed as mean ± standard deviation (n=3); compared with conventional alcohol extraction. P <0.05, P <0.001; One-way ANOVA was used to compare the overall differences in total flavonoid yield among the four groups.

[0041] Example 2 Preparation method of miracle fruit extract: (1) Take dried miracle fruit, remove the seeds, crush it through a 40-mesh sieve to obtain miracle fruit powder; (2) Take 1 kg of miracle fruit powder, add 15 g of compound enzyme (the mass ratio of cellulase and pectinase is 2:1), add 8 L of citrate buffer, adjust the pH to 5.0, and enzymatically hydrolyze at 50℃ for 90 minutes; (3) After the enzymatic hydrolysis is completed, the temperature is raised to 80℃ to inactivate the enzyme for 15 minutes, then ethanol is added to make the final ethanol concentration reach 70% (v / v), 3g ascorbic acid is added, and the mixture is refluxed at 70℃ for 2 hours. The filtrate is then collected by filtration. (4) The filtrate was concentrated under reduced pressure at 50°C and spray-dried to obtain miracle fruit extract.

[0042] Example 3 Low-temperature preparation of miracle fruit seed ultrafine powder: Take 200g of dried mystery fruit seeds, coarsely crush them, and then put them into a low-temperature ultrafine pulverizer. Pulverize them at -5℃ until the particle size D90 < 20 micrometers to obtain mystery fruit seed ultrafine powder.

[0043] Example 4 Preparation of a composition with uric acid-lowering effect: (1) Weigh the following raw materials by weight: 40 parts of miracle fruit leaf extract prepared in Example 1, 15 parts of miracle fruit fruit extract prepared in Example 2, 8 parts of miracle fruit seed ultrafine powder prepared in Example 3, 25 parts of chicory root powder, 15 parts of inulin and 4 parts of mogroside.

[0044] (2) Put the above raw materials into a three-dimensional mixer and mix for 30 minutes to ensure they are fully mixed. Then, pass the mixture through a 60-mesh sieve. Pack the sieve material into bags of 5g each to obtain the finished product.

[0045] Example 5 Preparation of a composition with uric acid-lowering effect: (1) Weigh the following raw materials by weight: 30 parts of miracle fruit leaf extract prepared in Example 1, 20 parts of miracle fruit fruit extract prepared in Example 2, 5 parts of miracle fruit seed ultrafine powder prepared in Example 3, 30 parts of chicory root powder, 10 parts of inulin and 5 parts of mogroside.

[0046] (2) Put the above raw materials into a three-dimensional mixer and mix for 30 minutes to ensure they are fully mixed. Then, pass the mixture through a 60-mesh sieve. Pack the sieve material into bags of 5g each to obtain the finished product.

[0047] Example 6 Preparation of a composition with uric acid-lowering effect: (1) Weigh the following raw materials by weight: 50 parts of miracle fruit leaf extract prepared in Example 1, 10 parts of miracle fruit fruit extract prepared in Example 2, 10 parts of miracle fruit seed ultrafine powder prepared in Example 3, 20 parts of chicory root powder, 20 parts of inulin and 2 parts of mogroside.

[0048] (2) Put the above raw materials into a three-dimensional mixer and mix for 30 minutes to ensure they are fully mixed. Then, pass the mixture through a 60-mesh sieve. Pack the sieve material into bags of 5g each to obtain the finished product.

[0049] Example 7 1. Experimental Materials (1) Xanthine oxidase (XO): purchased from Sigma-Aldrich, with a specific activity ≥0.4 U / mg, prepared to 0.1 U / mL with 0.1 M phosphate buffer (pH 7.5).

[0050] (2) Xanthine: purchased from Sigma-Aldrich, purity ≥99%, prepared into 0.5 mM using 0.1 M phosphate buffer (pH 7.5).

[0051] (3) Allopurinol (positive control): purity ≥98%, purchased from Sigma-Aldrich, dissolved in DMSO and serially diluted.

[0052] (4) Sample to be tested: Miracle fruit leaf extract prepared in Example 1; Miracle fruit extract prepared in Example 2; Miracle fruit seed ultrafine powder prepared in Example 3; Composition group: The miracle fruit leaf extract obtained in Example 1, the miracle fruit fruit extract obtained in Example 2, and the miracle fruit seed ultrafine powder obtained in Example 3 are mixed evenly at a mass ratio of 40:15:8.

[0053] (5) Blank control: 0.1 M phosphate buffer (pH 7.5) (containing 0.1% DMSO).

[0054] 2. Experimental Methods (1) Sample gradient dilution Each sample was serially diluted with DMSO, and the final concentrations in the reaction system were set as follows: 200, 100, 50, 25, 12.5, 6.25, 3.125, and 0 μg / mL.

[0055] The final concentrations of allopurinol were set as follows: 10, 5, 2.5, 1.25, 0.625, 0.3125, 0.15625, and 0 μmol / L.

[0056] Three replicates were set for each concentration.

[0057] (2) Reaction system (total volume 200 μL / well), see Table 5 Table 5 Components and dosages of the reaction system (3) Detection and Calculation Mix PBS (0.1 M, pH 7.5), the sample solution, and XO enzyme solution, and pre-incubate at 37°C for 10 minutes; then add xanthine substrate, mix well, and react at 37°C for 30 minutes; immediately after the reaction, measure the absorbance (OD) at 295 nm using a microplate reader. 295 Background control: Contains only PBS and the sample solution, without enzymes or substrates.

[0058] Inhibition rate calculation formula: Inhibition rate (%) = (OD) 空白对照 -OD 样品 ) / (OD 空白对照 -OD 背景 ) × 100%; Plotting the logarithm of sample concentration (log C) on the x-axis and the inhibition rate on the y-axis, a nonlinear regression (GraphPad Prism 8.0, four-parameter logistic model) was used to fit the dose-response curve, and the IC was calculated. 50 .

[0059] Each sample was tested independently and repeated 3 times. The results are expressed as mean ± standard deviation.

[0060] (4) Synergistic effect evaluation method (Chou-Talalay joint index method) For a three-component combination, the combination index CI is calculated when a certain effect level (e.g., 50% inhibition rate) is reached: ; in: D A,comb The actual concentration at which the miraculous fruit leaf extract component in the composition achieves 50% inhibition (i.e., the IC50 concentration of the composition). 50 × Mass fraction of miracle fruit leaf extract in the combination); D A,alone IC50 when miracle fruit leaf extract alone achieves 50% inhibition 50 ; D B,combThe actual concentration at which the miracle fruit extract component in the composition achieves 50% inhibition (i.e., the IC50 concentration of the composition). 50 × Mass fraction of miracle fruit extract in the composition); D B,alone IC50 when miracle fruit extract alone achieves 50% inhibition 50 ; D C,comb The actual concentration of the miracle fruit seed ultrafine powder component in the composition when it reaches 50% inhibition (i.e., the composition IC50). 50 × Mass fraction of miracle fruit seed ultrafine powder in the composition); D C,alone When miracle fruit seed ultrafine powder alone achieves 50% inhibition of IC50 50 .

[0061] CI value criteria: CI < 0.7: significant synergistic effect; 0.7 ≤ CI < 0.9: moderate synergistic effect; 0.9 ≤ CI ≤ 1.1: additive effect; CI > 1.1: antagonistic effect.

[0062] 3. Experimental Results: IC50 of each sample group for XO 50 See Table 6. The synergy index (CI) calculation results are shown in Table 7. The CI of the composition group is 0.410 < 0.7, which is considered to be a significant synergistic effect.

[0063] Table 6 IC50 of xanthine oxidase in each group of samples 50 value Note: Allopurinol IC50 50 It has extremely low levels of harmful substances, but it has side effects such as liver toxicity. The advantage of this invention lies in its natural safety.

[0064] Table 7. Compositions of the present invention in IC 50 Level of Coordination Index (CI) Calculation Example 8 Animal efficacy studies: Experimental animals were SPF-grade male Kunming mice, weighing approximately 18–22 g. These mice were randomly divided into six groups of 10 mice each: a normal control group, a model group, a positive control group (allopurinol 10 mg / kg), the low-dose group of this invention, the medium-dose group of this invention, and the high-dose group of this invention. Under normal diet and water conditions, except for the normal control group, the other groups received intraperitoneal injections of the uricase inhibitor potassium oxonate at a dose of 250 mg / kg for 8 consecutive days to establish a hyperuricemia model. Two hours after each modeling, the normal control group and the model group were given an equal volume of physiological saline, the positive control group was given allopurinol, and the other groups were administered the composition prepared in Example 4 by gavage at the corresponding doses (see Table 8) for 7 consecutive days. Changes in serum uric acid levels in each group were observed, and the results are shown in Table 8.

[0065] The results showed that the composition with uric acid-lowering effect prepared by the present invention could effectively reduce serum uric acid levels in mice, inhibit liver XO activity, and reduce IL-1β levels, without significant liver and kidney damage.

[0066] Table 8. Effects of the composition of the present invention on serum biochemical indicators and hepatic xanthine oxidase in hyperuricemia model mice. ±SD, n=10) Note: Low, medium, and high doses are calculated as 5 times, 10 times, and 20 times the human dose, respectively; compared with the normal control group: ## P <0.01, ### P <0.001 (indicating successful modeling); Comparison with the model group: P <0.05, P <0.01, P <0.001 (indicating significant therapeutic effect).

[0067] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A composition having a uric acid-lowering effect, characterized in that, It includes the following components by weight: 30-50 parts miracle fruit leaf extract, 10-20 parts miracle fruit fruit extract, and 5-10 parts miracle fruit seed ultrafine powder.

2. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: 40 parts of miracle fruit leaf extract, 15 parts of miracle fruit fruit extract, and 8 parts of miracle fruit seed ultrafine powder.

3. The composition according to claim 1, characterized in that, The composition further includes the following components in parts by weight: 20-30 parts chicory root powder, 10-20 parts inulin, and 2-5 parts mogroside.

4. The composition according to claim 3, characterized in that, The composition comprises the following components in parts by weight: 40 parts of miracle fruit leaf extract, 15 parts of miracle fruit fruit extract, 8 parts of miracle fruit seed ultrafine powder, 25 parts of chicory root powder, 15 parts of inulin, and 4 parts of mogroside.

5. The composition according to any one of claims 1-4, characterized in that, The preparation method of the miracle fruit leaf extract or the miracle fruit fruit extract includes the following steps: After enzymatic hydrolysis of mirabilis leaves or fruits using a compound enzyme, the enzyme is inactivated to obtain the hydrolysate. Ethanol and ascorbic acid were added to the enzymatic hydrolysate, and the mixture was refluxed and extracted, then filtered to obtain the filtrate. The filtrate is dried to obtain the miracle fruit leaf extract or the miracle fruit fruit extract; The complex enzyme includes cellulase and pectinase.

6. The composition according to claim 5, characterized in that, The mass ratio of the cellulase to the pectinase is 2:1; and / or The compound enzyme is present in a mass ratio of 0.6-2.5% relative to the leaves or fruit of the miracle fruit.

7. The composition according to claim 5, characterized in that, The ascorbic acid is present in a mass ratio of 0.1-0.5% relative to the leaves or fruit of the miracle fruit.

8. A method for preparing the composition according to any one of claims 1-7, characterized in that, The process includes the step of mixing the components evenly to obtain the composition.

9. The use of the composition according to any one of claims 1-7 in the preparation of a uric acid-lowering drug.

10. A uric acid-lowering drug, characterized in that, The active ingredient includes the composition according to any one of claims 1-7.

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