Method and system for coupling biotransformation function body stress cycle breeding and sewage zero discharge

Abstract

This invention discloses a method and system for coupled biotransformation functional organism stress-induced circular aquaculture and zero wastewater discharge. The method first lays a granular absorbent carrier on a multi-layer aquaculture device to form a carrier layer, then sprays organic wastewater onto it to adsorb and form a moist biofilm. Subsequently, a biotransformation functional organism capable of converting organic matter is introduced into the carrier layer, and suitable humidity is maintained through cyclic spraying, driving the organism to consume pollutants and undergo biotransformation. Finally, the system separates and recovers the transformed biological products and reusable carrier. This invention breaks with the traditional concept that wastewater treatment is a cost center. Through process innovation, driven and realized by a single time-controlled ventilation and humidity control system, the wastewater treatment process itself is transformed into a value-added link that simultaneously produces high-value biological products. This not only solves environmental problems but also creates significant economic benefits, achieving a unity of environmental and economic benefits.

Description

Technical Field

[0001] This invention belongs to the field of environmental protection and resource recycling technology, specifically relating to a method and system for coupled biotransformation functional body stress-recycled aquaculture and zero wastewater discharge, which is particularly suitable for treating high-concentration organic wastewater, such as leachate from kitchen waste and aquaculture wastewater. Background Technology

[0002] The use of bioconversion technology to treat organic wastewater and achieve resource recovery is a current research hotspot. Saprophytic insects, represented by the black soldier fly, are widely used to treat high-concentration organic waste such as kitchen waste, livestock manure, and food processing wastewater due to their broad diet and high conversion efficiency. Existing technologies, such as the Chinese patent with publication number CN104926017B, involve adding powdered absorbent plant materials (such as wheat bran) to wastewater to solidify it into a viscous material for rearing. However, this method suffers from carrier caking, poor air permeability, reliance on ground-based fermentation beds for leachate treatment, and a fragmented process flow with low automation. While the Chinese patent with publication number CN116267803B introduces porous media (such as biochar and ceramsite) and simplifies pretreatment, its core process remains static batch processing, lacking active and precise control over the rearing microenvironment (such as humidity and oxygen), thus limiting treatment efficiency and large-scale application. The biological essence of the black soldier fly larvae is that of an organic matter converter. Its main function is to convert suspended solids and dissolved organic matter (COD) or other solid components in wastewater into insect biomass and insect excrement (solid feces) through ingestion and digestion. In this process, water molecules themselves are not eliminated; they are simply transferred from the free-flowing liquid wastewater and redistributed into the insect tissue water, the water bound to the insect excrement, and the saturated air humidity. The total amount of water input into the system is almost entirely retained within the system. Therefore, normal black soldier fly aeration farming has no significant effect on wastewater reduction.

[0003] Meanwhile, the above methods mainly focus on the reduction of pollutants and the production of basic insect proteins, failing to actively intervene in and improve the intrinsic quality and added value of black soldier fly larvae. Summary of the Invention

[0004] This invention provides a method and system for coupling biotransformation functional body stress-cycled aquaculture with zero wastewater discharge, which solves the problems of insignificant wastewater reduction and low quality of black soldier fly larvae in existing wastewater treatment processes.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows: A method for coupling biotransformation functional organism stress-induced aquaculture with zero wastewater discharge includes the following steps: S1. Carrier pretreatment and loading: The granular water-absorbing carrier material is laid on the breeding layer of the multi-layer three-dimensional aquaculture equipment to form a carrier layer; S2. Wastewater spraying and adsorption: Organic wastewater is sprayed onto the carrier layer, causing it to adsorb and form a moist biofilm layer; S3. Inoculation and transformation: Introduce biotransformation functional organisms that can consume, degrade, or transform organic matter and nutrients in wastewater into the carrier layer; S4. Circulating spraying and bioconversion: During the breeding cycle, spraying is carried out in a circulatory manner to maintain the humidity of the carrier layer, so that the bioconversion functional body consumes organic matter and converts it. S5: Ventilation: A top-to-bottom penetrating ventilation control is implemented for the carrier layer, the ventilation control comprising sequential execution of: During the spraying phase, close the ventilation or maintain a minimum ventilation rate; During the conversion phase after spraying, a micro-ventilation mode is activated, introducing a low-speed airflow from the bottom of the carrier layer. After the transformation is completed, switch to strong ventilation mode and introduce high-speed through airflow from the bottom of the carrier layer to reduce the humidity of the carrier layer and create controllable abiotic stress on the biotransformation functional organism. S6. Product Separation and Recovery: After the breeding cycle is completed, the biotransformation functional products and water-absorbing carrier materials are separated and recovered.

[0006] Furthermore, preferably, the timing of the release of the biotransformation functional body includes: simultaneous release: the biotransformation functional body is released simultaneously with the laying of the carrier layer; or delayed release: the biotransformation functional body is released after the water-absorbing carrier layer is laid or after the sewage spraying and adsorption in step S2 has formed a stable moist biofilm layer.

[0007] Furthermore, preferably, the strong ventilation mode performs the following functions: rapidly removes moisture to complete wastewater purification, and reduces the humidity of the carrier layer to a predetermined threshold to stimulate the biotransformation function to increase the content of specific active substances in the body.

[0008] Furthermore, preferably, the biotransformation functional organism is one or more of insect larvae, microbial preparations, or suitable plants.

[0009] Furthermore, preferably, the insect larvae are black soldier fly larvae or fly maggot larvae.

[0010] Further, preferably: the absorbent carrier is a solid material with a porous structure, high specific surface area, and good water retention; it includes, but is not limited to: a) Industrial by-products or waste: biochar, coal gasification furnace slag, brewing lees, edible fungi residue, sugarcane bagasse, citric acid fermentation waste residue; b) Agricultural and forestry residues: corn cobs, rice husks, wheat straw, cotton stalks, coconut coir, sawdust, bamboo powder, peanut shells; c) Mining by-products or processed products: bentonite, perlite, vermiculite, zeolite, diatomite; d) Synthetic or recycled materials: polyurethane foam fragments, regenerated cellulose materials, superabsorbent polymers; e) Other natural or modified materials: weathered coal, humic acid matrix materials.

[0011] Furthermore, preferably, the wastewater includes domestic sewage, livestock and poultry manure wastewater, food processing wastewater, industrial organic wastewater, and landfill leachate.

[0012] Further, preferably, the particle size of the granular water-absorbing carrier material is larger than the sieve particle size of the large biotransformation functional body.

[0013] This invention also provides a system for coupled biotransformation functional body stress-cycled aquaculture and zero wastewater discharge, including multiple layers of aquaculture layers with automatic feeding and discharging. Each aquaculture layer is equipped with a spraying mechanism at the top and a wind guide plate at the bottom. The wind guide plate is sealed at one end adjacent to the air outlet side and inclined downward at the other end adjacent to the air inlet side to form a ventilation duct.

[0014] Furthermore, preferably: a water collection trough is provided at the air inlet side end of the air guide plate, the water collection trough is connected to the return water pipe, and the return water pipe is connected to the sewage tank; And / or the aforementioned spraying mechanism is connected to the sewage tank via a water supply mechanism.

[0015] The beneficial effects of this invention are: The method of this invention adsorbs harmful substances and nutrients in wastewater through a carrier, which then transforms them into biotransformation functional products. The carrier is recycled, and no harmful organic matter needs to be treated or discharged as waste, achieving zero waste of pollutants. At the same time, the method of this invention does not rely on complex membrane filtration or deep treatment to produce liquid discharge water. Instead, it uses strong ventilation to force the water in the wastewater from liquid to gas (water vapor) and discharge it from the system. The water-absorbing carrier provides a huge evaporation area, and the vertical airflow efficiently carries moisture. The entire process achieves full gas-phase removal of the input water. In the end, only humid air is discharged from the system, meeting the strict standard of zero liquid discharge.

[0016] The method of this invention introduces strong ventilation-drying as a controllable abiotic stress. This alternating wet and dry stress condition can stimulate the stress defense mechanism of biotransformation functional organisms, resulting in a significant increase in the content of secondary metabolites with high nutritional or medicinal value, such as antimicrobial peptides, functional proteins, specific amino acids, or polyunsaturated fatty acids. This makes the produced biotransformation functional organisms no longer ordinary feed protein, but potential functional feed additives or sources of bioactive substances, thus multiplying their value.

[0017] This invention breaks with the traditional view that wastewater treatment is a cost center. Through process innovation, driven and realized by the same time-controlled ventilation and humidity control system, the wastewater treatment process itself is transformed into a value-added link that simultaneously produces high-value biological products. This not only solves environmental problems but also creates significant economic benefits, achieving a unity of environmental and economic benefits. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the system of the present invention, which combines coupled biotransformation functional body stress-induced cyclic aquaculture with zero wastewater discharge; In the diagram, 1 is the ventilation system, 2 is the return water pipe, 3 is the water collection tank, 4 is the air guide plate, 5 is the spray head, 6 is the spray pipe, 7 is the aquaculture layer, 8 is the sewage tank, and 9 is the water supply system. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are also described.

[0021] A method for coupling biotransformation functional organism stress-induced aquaculture with zero wastewater discharge includes the following steps: S1. Carrier pretreatment and loading: The granular water-absorbing carrier material is laid on the breeding layer of the multi-layer three-dimensional aquaculture equipment to form a carrier layer; S2. Wastewater spraying and adsorption: Organic wastewater is sprayed onto the carrier layer, causing it to adsorb and form a moist biofilm layer; S3. Inoculation and transformation: Introduce biotransformation functional organisms that can consume, degrade, or transform organic matter and nutrients in wastewater into the carrier layer; S4. Circulating spraying and bioconversion: During the breeding cycle, spraying is carried out in a circulatory manner to maintain the humidity of the carrier layer, so that the bioconversion functional body consumes organic matter and converts it. S5: Ventilation: A top-to-bottom penetrating ventilation control is implemented for the carrier layer, the ventilation control comprising sequential execution of: During the spraying phase, close the ventilation or maintain a minimum ventilation rate; During the conversion phase after spraying, a micro-ventilation mode is activated, introducing a low-speed airflow from the bottom of the carrier layer to maintain a basic oxygen supply inside the carrier layer, while maintaining a high level of environmental humidity. The biotransformation functional organisms feed and grow in this stable and humid environment. After the conversion is completed, switch to strong ventilation mode and introduce high-speed through airflow from the bottom of the carrier layer to reduce the humidity of the carrier layer and create controllable abiotic stress on the bioconversion functional body, stimulate the metabolic pathways in the body to change, thereby increasing the accumulation and content of specific active substances in the body, and ultimately improving the quality and value of the bioconversion functional body. At the same time, the airflow quickly removes moisture, completing the treatment and conversion of wastewater. S6. Product Separation and Recovery: After the breeding cycle is completed, the biotransformation functional products and water-absorbing carrier materials are separated and recovered.

[0022] Biotransformers are organisms that can consume, degrade, or transform organic matter and nutrients in wastewater. They generally include one or more of the following: insect larvae, microbial preparations, or suitable plants.

[0023] The insect larvae are black soldier fly larvae and fly maggot larvae. These larvae directly feed on suspended organic matter, colloidal substances, microbial communities, and some soluble substances attached to carriers in sewage through their mouthparts. The enzymes secreted by their digestive tract (protease, lipase, amylase, etc.) and the rich intestinal microbiota decompose complex organic matter into absorbable nutrients and solidify liquid or easily lost pollutants into insect body tissue (biomass rich in protein and fat) and insect sand (structurally stable organic fertilizer).

[0024] Microbial preparations mainly mineralize or transform pollutants into cellular substances, such as bacteria, fungi, and actinomycetes. They are generally loaded onto carriers and then released into the culture layer.

[0025] Suitable plants are generally aquatic plants or moisture-tolerant higher plants. The plant roots secrete sugars, organic acids and other substances, forming a rich microbial zone (rhizosphere microbial community) around the roots. The number and activity of these microorganisms are much higher than those in non-rhizosphere soil. These microorganisms are the main executors of pollutant degradation.

[0026] The aforementioned biotransformation functional organisms can be selected according to different situations. The embodiments of the present invention take the black soldier fly larva as an example to specifically illustrate the solution of the present invention.

[0027] The absorbent carrier is a solid material with a porous structure, high specific surface area, and good water retention capacity; it includes, but is not limited to: a) Industrial by-products or waste: biochar, coal gasification furnace slag, brewing lees, edible fungi residue, sugarcane bagasse, citric acid fermentation waste residue; b) Agricultural and forestry residues: corn cobs, rice husks, wheat straw, cotton stalks, coconut coir, sawdust, bamboo powder, peanut shells; c) Mining by-products or processed products: bentonite, perlite, vermiculite, zeolite, diatomite; d) Synthetic or recycled materials: polyurethane foam fragments, regenerated cellulose materials, superabsorbent polymers; e) Other natural or modified materials: weathered coal, humic acid matrix materials.

[0028] The choice of absorbent carriers for the different materials mentioned above can be made according to specific circumstances. For example, mining by-products or processed products: bentonite, perlite, vermiculite, zeolite, diatomaceous earth; synthetic or recycled materials: polyurethane foam fragments, regenerated cellulose materials, superabsorbent resins; these carriers generally do not contain nutrients, but they have good support and air permeability, and are easy to air dry and remove water.

[0029] Industrial by-products or waste: biochar, coal gasification slag, brewing lees, edible fungi residue, sugarcane bagasse, citric acid fermentation waste; b) Agricultural and forestry residues: corn cobs, rice husks, wheat straw, cotton stalks, coconut coir, sawdust, bamboo powder, peanut shells; other natural or modified materials: weathered coal, humic acid matrix materials. These types of carriers contain certain nutrients, which can improve the efficiency of aquaculture, and these materials are organic matter that can be recycled.

[0030] Wastewater includes domestic sewage, livestock and poultry manure wastewater, food processing wastewater, industrial organic wastewater, and landfill leachate. Before spraying the above-mentioned wastewater, it is pre-treated according to the living habits of organisms, such as filtering, removing heavy metals, oils, etc., to make it suitable for the nutritional needs of biotransformation organisms.

[0031] like Figure 1As shown, a system coupling biotransformation functional body stress-based cyclic aquaculture and zero wastewater discharge includes a multi-layered, automatically feeding and discharging aquaculture layer 7. The number of aquaculture layers can be designed according to the specific wastewater volume to be treated; in this embodiment, there are 5 layers. The aquaculture layer 7 and its accessories are existing technologies, such as the frame, ventilation mechanism 1, and exhaust gas treatment mechanism. For specific details, refer to application numbers: 202510903409.2, invention title: A precision-controlled assembly line-style three-dimensional aquaculture system for insects; application number: 2025109034158, invention title: A zoned continuous aquaculture mechanism and its aquaculture system; and application number: 2025120351263, invention title: A micro-powered, detachable, three-dimensional continuous aquaculture system for insects, and other related technologies.

[0032] Each of the breeding layers 7 is equipped with a spraying mechanism at the top and an air guide plate 4 at the bottom. The end of the air guide plate 4 adjacent to the air outlet side is sealed, and the end adjacent to the air inlet side is inclined downward to form a ventilation duct.

[0033] A water collection trough 3 is provided at the air inlet end of the air guide plate 4. The water collection trough 3 is connected to the return water pipe 2, and the return water pipe 2 is connected to the sewage tank 8. The sewage dripping during the spraying process is collected and reused. The spraying mechanism is connected to the sewage tank 8 through a water supply mechanism 9. The water supply mechanism is generally a water pump and its control system, which is existing technology and will not be described in detail. Appropriate equipment can be selected according to needs. The spraying mechanism generally includes a spray pipe 6 and a spray head 5, which is existing technology and will not be described in detail.

[0034] Experimental Example 1 Using the above system as the breeding equipment, corn cob particles and rice husks are mixed evenly in a volume ratio of 4:6 as a water-absorbing carrier. Before spreading the material, the carrier has a moisture content of 16.5%. Five-day-old black soldier fly larvae are used as the biological transformation functional body. Each breeding layer has a width of 2m, a length of 8m, and a thickness of 15cm. The insect feeding rate is 10%. Spraying is used to reduce the moisture content of the water-absorbing carrier to about 80%. Ventilation is maintained at a wind speed of 0.1-0.2 m / s for 12 hours. Then, the carrier is air-dried at a wind speed of 2-3 m / s to reduce the moisture content to about 50%. The above process is repeated for 8 days. After the breeding is completed, the moisture content of the air-dried carrier is below 50%. Then, the black soldier fly larvae, insect sand, and water-absorbing carrier are screened out.

[0035] The wastewater from a certain winery has the following parameters: pH: 6.05; SS / (mg / L): 3862.5; COD / (mg / L): 12314.6; BOD / (mg / L): 7125.8; NH3-N / (mg / L): 321.4; TP / (mg / L): 35.6.

[0036] Comparative Example 1 The experiment was basically the same as in Experiment 1, except that after 12 hours of cultivation, the substrate was not air-dried, but sprayed with water to keep the moisture content of the absorbent carrier at around 80%.

[0037] Comparative Example 2 The experiment was basically the same as in Experiment 1, except that after 12 hours of cultivation, the substrate was air-dried to reduce the water-absorbing carrier to about 60%, and then sprayed with the substrate to bring the water-absorbing carrier to about 80% moisture content.

[0038] Data measurement The amount of wastewater consumed, the weight of black soldier fly larvae harvested from each breeding layer 7, and the antimicrobial peptide content of the black soldier fly larvae were calculated. The specific results are shown in Table 1.

[0039] Determination of antimicrobial peptides in black soldier fly larvae: Extraction of antimicrobial peptides from black soldier fly larvae: Black soldier fly larvae dried in different examples were repeatedly rinsed with distilled water and disinfected with 75% ethanol. The surface liquid of the larvae was then absorbed. 10g of black soldier fly larvae were weighed and placed in a mortar. Extraction solution (0.05mol / L pH=5 ammonium acetate buffer, 35μg / mL benzyl sulfonyl fluoride PMSF, 2‰ mercaptoethanol) was added at a weight / volume ratio of 1:5. The mixture was thoroughly ground, and the homogenate was centrifuged at 10000r / min for 30min at 4℃. The supernatant was collected, and this process was repeated twice. The supernatants were combined and then placed in a 100℃ constant temperature water bath for 5min. Afterward, the mixture was centrifuged at 5000r / min (4℃) for 30min to remove denatured proteins. The supernatant without an oil layer was extracted using a sterile syringe and freeze-dried to obtain the crude antimicrobial peptide extract.

[0040] Table 1 Experimental results of different embodiments

[0041] As shown in the table above, within the same time period, the method of the present invention reduces the most wastewater, has the highest wastewater conversion ratio (50.1), and the highest conversion efficiency; the antimicrobial peptide content is 1.12 mg / g, which is more than 10 times that of Comparative Example 1 and more than 3 times that of Comparative Example 2.

[0042] In Comparative Example 1, ventilation was carried out during the breeding process. The amount of wastewater generated through evaporation and insect transformation was relatively small, resulting in the water-absorbing carriers consistently handling high water content. Consequently, the nutrient supply could not be replenished by adding fresh wastewater, leading to a long-term state of nutrient deficiency for the black soldier fly larvae. This negatively impacted the yield and antimicrobial peptide content of the black soldier fly larvae.

[0043] Comparative Example 2 involved air-drying during the breeding process without affecting the growth of black soldier fly larvae. Its wastewater conversion ratio was not much different from that of Experimental Example 1, but its antimicrobial peptides were significantly different from those of Experimental Example 1, being only 33% of those of Experimental Example 1.

[0044] It is evident that the process of this invention can not only effectively treat wastewater, but also effectively increase the antimicrobial peptide content of black soldier fly larvae, thereby significantly enhancing their value.

[0045] During the drying stage, the wastewater on the carrier is transformed by black soldier fly larvae, and there are basically no pollutants. Therefore, the exhaust gas from this stage is mostly water vapor, which can be directly discharged after passing the test.

[0046] Experiment Example 2 A black soldier fly larvae farming experiment was conducted using coal gasification furnace slag, bentonite, and brewing lees as absorbent carriers in a volume ratio of 3:1:6, and wastewater from a certain aquaculture farm as the nutrient source. The specific equipment and process steps were the same as in Example 1.

[0047] The wastewater from a certain winery has the following parameters: pH: 5.69; SS / (mg / L): 12631.2; COD / (mg / L): 20158.6; BOD / (mg / L): 8013.2; NH3-N / (mg / L): 386.7; TP / (mg / L): 41.6.

[0048] Multiple batches of aquaculture trials were conducted. After each aquaculture, the water-absorbing carrier was screened and recovered, and the experimental results were measured. The details are shown in Table 2.

[0049] Table 2 Results of different batches of aquaculture trials

[0050] As shown in Table 2, the antimicrobial peptide content of the four batches of black soldier fly larvae was similar, all around 1.10 mg / g, and the wastewater conversion ratio of each batch was around 48, which was stable.

[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for coupling biotransformation functional body stress-induced cyclic aquaculture with zero wastewater discharge, characterized in that, Includes the following steps: S1. Carrier pretreatment and loading: The granular water-absorbing carrier material is laid on the breeding layer of the multi-layer three-dimensional aquaculture equipment to form a carrier layer; S2. Wastewater spraying and adsorption: Organic wastewater is sprayed onto the carrier layer, causing it to adsorb and form a moist biofilm layer; S3. Inoculation and transformation: Introduce biotransformation functional organisms that can consume, degrade, or transform organic matter and nutrients in wastewater into the carrier layer; S4. Circulating spraying and bioconversion: During the breeding cycle, spraying is carried out in a circulatory manner to maintain the humidity of the carrier layer, so that the bioconversion functional body consumes organic matter and converts it. S5: Ventilation: A top-to-bottom penetrating ventilation control is implemented for the carrier layer, the ventilation control comprising sequential execution of: During the spraying phase, close the ventilation or maintain a minimum ventilation rate; During the conversion phase after spraying, a micro-ventilation mode is activated, introducing a low-speed airflow from the bottom of the carrier layer. After the transformation is completed, switch to strong ventilation mode and introduce high-speed through airflow from the bottom of the carrier layer to reduce the humidity of the carrier layer and create controllable abiotic stress on the biotransformation functional organism. S6. Product Separation and Recovery: After the breeding cycle is completed, the biotransformation functional products and water-absorbing carrier materials are separated and recovered.

2. The method according to claim 1, characterized in that: The timing of the release of the biotransformation functional body includes: simultaneous release: the biotransformation functional body is released simultaneously with the laying of the carrier layer; or delayed release: the biotransformation functional body is released after the water-absorbing carrier layer is laid or after the sewage spraying and adsorption in step S2 has formed a stable moist biofilm layer.

3. The method according to claim 1, characterized in that: The strong ventilation mode performs the following functions: rapidly removes moisture to complete wastewater purification, and reduces the humidity of the carrier layer to a predetermined threshold to stimulate the biotransformation function to increase the content of specific active substances in the body.

4. The method according to any one of claims 1-3, characterized in that: The biotransformation functional organism is one or more of insect larvae, microbial preparations, or suitable plants.

5. The method according to claim 4, characterized in that: The insect larvae mentioned are black soldier fly larvae or fly maggot larvae.

6. The method according to any one of claims 1-3, characterized in that, The absorbent carrier is a solid material with a porous structure, high specific surface area, and good water retention; it includes, but is not limited to: a) Industrial by-products or waste: biochar, coal gasification furnace slag, brewing lees, edible fungi residue, sugarcane bagasse, citric acid fermentation waste residue; b) Agricultural and forestry residues: corn cobs, rice husks, wheat straw, cotton stalks, coconut coir, sawdust, bamboo powder, peanut shells; c) Mining by-products or processed products: bentonite, perlite, vermiculite, zeolite, diatomite; d) Synthetic or recycled materials: polyurethane foam fragments, regenerated cellulose materials, superabsorbent polymers; e) Other natural or modified materials: weathered coal, humic acid matrix materials.

7. The method according to any one of claims 1-3, characterized in that: The wastewater includes domestic sewage, livestock and poultry manure wastewater, food processing wastewater, industrial organic wastewater, and landfill leachate.

8. The method according to any one of claims 1-3, characterized in that: The particle size of the granular water-absorbing carrier material is larger than the sieve particle size of the large biotransformation functional body.

9. A system for coupling biotransformation functional body stress-induced cyclic aquaculture with zero wastewater discharge, characterized in that: The aquaculture layer includes multiple layers with automatic feeding and discharging. Each layer is equipped with a spraying mechanism at the top and an air guide plate at the bottom. The end of the air guide plate adjacent to the air outlet is sealed, and the end adjacent to the air inlet is inclined downward to form a ventilation duct.

10. The system according to claim 1, characterized in that: The air guide plate is provided with a water collection trough at the air inlet side end, the water collection trough is connected to the return water pipe, and the return water pipe is connected to the sewage tank. And / or the aforementioned spraying mechanism is connected to the sewage tank via a water supply mechanism.

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