A secondary pressure-holding transfer treatment device and method for microbial culture of deep-sea pressure-holding core samples

Abstract

This application provides a secondary pressure-holding transfer processing device and method for deep-sea pressure-holding core microbial culture. The processing device includes a horizontal pushing mechanism, a dual-valve pressure-holding transfer chamber, a vertical pushing mechanism, a circumferentially cut autoclave, a microbial culture chamber, a first pressure regulating mechanism, and a second pressure regulating mechanism. The first and second pressure regulating mechanisms ensure that the pressure in each cavity is equal. This allows the core to remain under pressure during the transfer process. The first and second control valves are opened. The core in the dual-valve pressure-holding transfer chamber is pushed into the circumferentially cut autoclave by the horizontal pushing mechanism. Inside the circumferentially cut autoclave, the core is cut using the autoclave and the vertical pushing mechanism. The cut core is then further pushed into the microbial culture chamber by the vertical pushing mechanism, achieving secondary pressure-holding transfer and propagation culture.

Description

Technical Field

[0001] This application relates to the field of pressure-sensitive microbial culture technology in high-pressure fields, and in particular to a secondary pressure-holding transfer treatment device and its treatment method suitable for microbial culture in deep-sea pressure-holding core samples. Background Technology

[0002] Deep-sea sediments are believed to contain over 60% of all bacterial biomass on Earth. However, due to technological limitations, research on pressure-sensitive microorganisms in high-pressure underground fields is still in its infancy. The habitat of deep-sea microorganisms, estimated to account for approximately 10% of global biomass, was only recently identified. Understanding the subsea biosphere may help determine the limits of life on Earth and aid in identifying biotechnologically useful organisms. The gradual development and maturation of marine pressure-controlled core sampling technology allows for the acquisition of in-situ pressure-controlled samples from the deep sea or underground wells, providing feasible conditions for obtaining pressure-sensitive microbial samples from deep-sea or high-pressure underground fields. However, currently, both pressure-controlled core samples and samples after pressure-controlled transfer are stored in plastic core tubes with relatively large diameters, which are unfavorable for microbial nutrient solution cultivation. Therefore, developing equipment for secondary pressure-controlled transfer processing of core samples to achieve bioactive culture conditions for pressure-controlled core samples and samples after pressure-controlled transfer without depressurization is of great significance. This invention provides a secondary pressure-controlled transfer processing device suitable for microbial culture in deep-sea pressure-controlled core samples. Summary of the Invention

[0003] This application provides a secondary pressure-holding transfer treatment device and method for deep-sea core microbial culture, which is used for secondary pressure-holding transfer treatment of deep-sea core microbial culture.

[0004] In a first aspect, embodiments of this application provide a secondary pressure-holding transfer processing device suitable for microbial culture in deep-sea pressure-holding core samples, comprising:

[0005] A horizontal pushing mechanism is used to move the core along the horizontal direction;

[0006] The dual-valve pressure-holding transfer chamber includes an outer shell assembly, a first control valve, and a second control valve. One end of the outer shell assembly is connected to a horizontal pushing mechanism. The first control valve and the second control valve are respectively sealed to the outer shell assembly. The first control valve and the second control valve are configured to control the opening or closing of the inner cavity of the outer shell assembly. The first control valve is located on the side of the second control valve facing the horizontal pushing mechanism, and the core is located between the first control valve and the second control valve.

[0007] The circumferential cutting autoclave, connected to the other end of the outer shell assembly, is configured to cut rock cores;

[0008] The vertical pushing mechanism is connected to the top of the ring-cutting autoclave. The vertical pushing mechanism is used to cut and push the core along the vertical direction.

[0009] The microbial culture chamber is connected to the bottom of the circumferential cutting autoclave and is configured to receive the cut core samples.

[0010] The first pressure regulating mechanism is configured to regulate the pressure in the cavity of the horizontal pushing mechanism and the pressure in the cavity of the first control valve in the housing assembly facing the horizontal pushing mechanism.

[0011] The second pressure regulating mechanism is configured to regulate the pressure inside the ring-cut autoclave, the vertical pushing mechanism, the microbial culture chamber, and the pressure inside the second control valve in the outer shell assembly on the side opposite to the horizontal pushing mechanism.

[0012] In some embodiments, the ring-cutting autoclave is provided with a horizontal inner cavity and a vertical inner cavity that are connected at right angles. The horizontal inner cavity is configured to accommodate a portion of the horizontal pushing mechanism, and the vertical inner cavity is configured to accommodate a portion of the vertical pushing mechanism.

[0013] The circumferential cutting autoclave includes a circumferential cutting head, which is located on the side of the horizontal inner cavity facing the vertical inner cavity. The cutting cavity of the circumferential cutting head is connected to both the horizontal and vertical inner cavities, and the diameter of the cutting cavity is smaller than the diameter of the core.

[0014] In some embodiments, the axis of the horizontal cavity is collinear with the axis of the housing assembly;

[0015] The axis of the cutting cavity is collinear with the axis of the circumferential cutting head and the axis of the horizontal inner cavity, respectively.

[0016] In some embodiments, the horizontal inner cavity is provided with an abutment structure, which is connected to the wall of the horizontal inner cavity. The abutment structure is located on the side of the circumferential cutting head facing the horizontal pushing mechanism. The abutment structure is used to abut the core tube to separate the core tube from the core.

[0017] In some embodiments, the circumferential cutting autoclave is provided with a waste chamber, which is located around the circumferential cutting head. The waste chamber is connected to a horizontal inner cavity, and the diameter of the waste chamber is larger than the diameter of the horizontal inner cavity.

[0018] In some embodiments, the vertical pushing mechanism includes a vertical pushing assembly and a pushing cutter head. The vertical pushing assembly is connected to the top of the ring-cutting autoclave, and a portion of the vertical pushing assembly is located within a vertical inner cavity. The pushing cutter head is located within the vertical inner cavity and is connected to the bottom of the vertical pushing assembly.

[0019] The vertical push assembly is configured to drive the push cutter head to move vertically. The push cutter head is configured to cut the core entering the vertical inner cavity from the cutting cavity and drive the core to move vertically downward to the stirring impeller in the microbial culture chamber. The stirring impeller is configured to rotate to cause the core to fall off the push cutter head and stir the core.

[0020] In some embodiments, the circumferential cutting autoclave is provided with an observation window, which is located on the side of the vertical inner cavity away from the horizontal inner cavity, and the observation window is opposite to the circumferential cutting head.

[0021] In some embodiments, the first pressure regulating mechanism includes a first water injection valve and a first air release valve, both of which are connected to the inner cavity of the horizontal pushing mechanism;

[0022] The second pressure regulating mechanism includes a second water injection valve, a first connecting valve, a second connecting valve, a second vent valve, and a third vent valve. The second water injection valve is connected to the vertical inner cavity. The first connecting valve is connected to the inner cavity of the microbial culture chamber and the second connecting valve, respectively. The second connecting valve is connected to the inner cavity of the vertical pushing mechanism. The second vent valve is connected to the inner cavity of the vertical pushing mechanism. The third vent valve is connected to the inner cavity of the ring-cutting autoclave and the first vent valve, respectively.

[0023] In some embodiments, the circumferential cutting autoclave is provided with a third control valve, which is disposed in the vertical inner cavity and located at the lower part of the vertical inner cavity; the second water injection valve is located above the third control valve;

[0024] The second pressure regulating mechanism also includes a third water injection valve, which is connected to the vertical inner cavity and is located below the third control valve.

[0025] The circumferential cutting autoclave is equipped with a first pressure relief valve, which is connected to the inner cavity of the circumferential cutting autoclave and is located below the third control valve.

[0026] The vertical pushing mechanism is equipped with a second pressure relief valve, which is connected to the first connecting valve and the second connecting valve respectively.

[0027] The microbial culture chamber includes a fourth control valve, which controls whether the inner cavity of the microbial culture chamber is connected to or not connected to the vertical inner cavity.

[0028] In a second aspect, embodiments of this application provide a secondary pressure-holding transfer treatment method suitable for microbial culture of deep-sea pressure-holding cores, which is used in the secondary pressure-holding transfer treatment device suitable for microbial culture of deep-sea pressure-holding cores provided in the first aspect above;

[0029] The methods include:

[0030] The pressure in the inner cavity of the horizontal pushing mechanism is made equal to the pressure in the inner cavity of the first control valve in the housing assembly facing the horizontal pushing mechanism by the first pressure regulating mechanism.

[0031] The pressure inside the ring-cut autoclave, vertical push mechanism, and microbial culture chamber is equalized by the second pressure regulating mechanism to the pressure inside the chamber on the side of the outer shell assembly opposite to the horizontal push mechanism.

[0032] Open the first control valve and the second control valve;

[0033] The horizontal pushing mechanism propels the core along the horizontal direction to the circumferential cutting autoclave, where the autoclave cuts the core.

[0034] The vertical pushing mechanism cuts the core sample after it has been cut by the high-pressure autoclave, and then pushes the core sample into the microbial culture chamber.

[0035] This application provides a secondary pressure-holding transfer processing device and method for deep-sea pressure-holding core microbial culture. The processing device includes a horizontal pushing mechanism, a dual-valve pressure-holding transfer chamber, a vertical pushing mechanism, a circumferentially cut autoclave, a microbial culture chamber, a first pressure regulating mechanism, and a second pressure regulating mechanism. The first pressure regulating mechanism ensures that the pressure inside the horizontal pushing mechanism is equal to the pressure inside the chamber of the outer shell assembly facing the first control valve. The second pressure regulating mechanism ensures that the pressure inside the circumferentially cut autoclave, the vertical pushing mechanism, and the microbial culture chamber is equal to the pressure inside the outer shell assembly facing away from the horizontal pushing mechanism from the second control valve. This ensures that the core remains under pressure during the transfer process. The first and second control valves are opened. The horizontal pushing mechanism pushes the core from the dual-valve pressure-holding transfer chamber into the circumferentially cut autoclave. Inside the circumferentially cut autoclave, the core is cut using the autoclave and the vertical pushing mechanism. The cut core is then further fed into the microbial culture chamber by the vertical pushing mechanism, achieving secondary pressure-holding transfer and propagation culture. Attached Figure Description

[0036] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application.

[0037] Figure 1 A schematic diagram of the secondary pressure-holding transfer processing device for microbial culture of deep-sea pressure-holding cores provided in this application;

[0038] Figure 2 Exploded view of the secondary pressure-holding transfer treatment device for microbial culture of deep-sea pressure-holding cores provided in this application;

[0039] Figure 3 for Figure 2 Cross-sectional view along the AA direction;

[0040] Figure 4 for Figure 2 Cross-sectional view along the BB direction;

[0041] Figure 5A schematic diagram showing the open state of the dual-valve pressure-holding transfer chamber in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea pressure-holding cores provided in this application;

[0042] Figure 6 A schematic diagram of the state of the core tube separated by the high-pressure autoclave in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea cores provided in this application;

[0043] Figure 7 A schematic diagram of the state of cutting the core in a high-pressure autoclave in a secondary pressure-holding transfer treatment device for microbial culture of deep-sea cores provided in this application;

[0044] Figure 8 A schematic diagram of the vertical pushing mechanism cutting the core in the secondary pressure-holding and transfer treatment device for microbial culture of deep-sea cores provided in this application;

[0045] Figure 9 A schematic diagram of the state of the vertical pushing mechanism driving the core movement in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea cores provided in this application;

[0046] Figure 10 for Figure 2 Cross-sectional view along the CC direction;

[0047] Figure 11 for Figure 2 Cross-sectional view along the DD direction;

[0048] Figure 12 A schematic diagram of the secondary pressure-holding transfer treatment method for microbial culture of deep-sea pressure-holding cores provided in this application;

[0049] Figure 13 A schematic diagram of the installation process of the secondary pressure-holding transfer treatment device for microbial culture of deep-sea pressure-holding cores provided in this application.

[0050] Explanation of reference numerals in the attached figures:

[0051] 100 - Horizontal pushing mechanism; 110 - First motor; 120 - First screw; 130 - First push nut; 140 - First high-pressure resistant pipe body; 150 - First sleeve; 160 - Push rod; 170 - First retaining ring installation and sealing joint; 180 - First retaining ring; 190 - High-pressure pipe installation and sealing joint; 1100 - First sealing joint;

[0052] 200 - Dual-valve pressure-holding transfer chamber; 210 - Outer shell assembly; 211 - First pressure-holding transfer chamber sealing connection joint; 212 - Second pressure-holding transfer sealing connection joint; 213 - Connecting pipe; 220 - First control valve; 230 - Second control valve;

[0053] 300-Circumferential cutting autoclave; 310-Horizontal inner cavity; 320-Vertical inner cavity; 330-Circumferential cutting head; 331-Cutting cavity; 340-Abutment structure; 350-Waste chamber; 360-Observation window; 370-High-pressure chamber body; 380-Flange sealing end cover; 390-Flange end cover; 3100-Third sealing connection joint; 3110-Third control valve; 3120-First pressure relief valve;

[0054] 400 - Vertical pushing mechanism; 410 - Vertical pushing assembly; 411 - Second sealing end cap; 412 - Second sealing connection joint; 413 - Connecting flange; 414 - Second retaining ring; 415 - Second motor; 416 - Second screw; 417 - Second push nut; 418 - Second high-pressure resistant pipe body; 419 - Second sleeve; 420 - Pushing cutter head; 430 - Second pressure relief valve;

[0055] 500 - Microbial culture chamber; 510 - Microbial culture chamber body; 520 - Fourth control valve; 530 - Agitator impeller; 540 - Third motor;

[0056] 600 - First pressure regulating mechanism; 610 - First water injection valve; 620 - First air vent valve;

[0057] 700 - Second pressure regulating mechanism; 710 - Second water injection valve; 720 - First connecting valve; 730 - Second connecting valve; 740 - Second vent valve; 750 - Third vent valve; 760 - High-pressure hose; 770 - Third water injection valve;

[0058] 800-core;

[0059] 900-core tube.

[0060] The accompanying drawings illustrate specific embodiments of this application, which will be described in more detail below. These drawings and descriptions are not intended to limit the scope of the concept in any way, but rather to illustrate the concept of this application to those skilled in the art through reference to particular embodiments. Detailed Implementation

[0061] Exemplary embodiments will now be described in detail, examples of which are illustrated in the accompanying drawings. When the following description relates to the drawings, unless otherwise indicated, the same numbers in different drawings denote the same or similar elements. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this application. Rather, they are merely examples of apparatuses and methods consistent with some aspects of this application as detailed in the appended claims.

[0062] It should be noted that the brief descriptions of terms in this application are only for the convenience of understanding the embodiments described below, and are not intended to limit the embodiments of this application. Unless otherwise stated, these terms should be understood in their ordinary and common meaning.

[0063] Furthermore, the terms “comprising” and “having”, and any variations thereof, are intended to cover but not exclusively include, for example, a product or device that includes a series of components is not necessarily limited to those that are explicitly listed, but may include other components that are not explicitly listed or that are inherent to such product or device.

[0064] In the description of this application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0065] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0066] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0067] In the secondary pressure-holding and transfer processing technology for deep-sea core microbial culture, it is necessary to collect deep-sea high-pressure cores under pressure and transfer them to the ship's laboratory, and then cut and transfer them to the high-pressure storage chamber for microbial culture.

[0068] Based on this, this application provides a secondary pressure-holding transfer processing device and method suitable for deep-sea pressure-holding core microbial culture. The processing device includes a horizontal pushing mechanism, a dual-valve pressure-holding transfer chamber, a vertical pushing mechanism, a circumferentially cut autoclave, a microbial culture chamber, a first pressure regulating mechanism, and a second pressure regulating mechanism. The first and second pressure regulating mechanisms ensure that the pressure in each cavity is equal. This allows the core to remain under pressure during the transfer process. The first and second control valves are opened. The core in the dual-valve pressure-holding transfer chamber is pushed into the circumferentially cut autoclave by the horizontal pushing mechanism. Inside the circumferentially cut autoclave, the core is cut using the autoclave and the vertical pushing mechanism. The cut core is then further pushed into the microbial culture chamber by the vertical pushing mechanism, achieving secondary pressure-holding transfer and propagation culture.

[0069] Figure 1 A schematic diagram of the secondary pressure-holding transfer processing device for microbial culture of deep-sea pressure-holding cores provided in this application. Figure 2 An exploded view of the secondary pressure-holding transfer treatment device for microbial culture of deep-sea pressure-holding cores provided in this application.

[0070] See Figure 1 and Figure 2 As shown in the figure, this application provides a secondary pressure-holding transfer processing device suitable for microbial culture of deep-sea pressure-holding core samples.

[0071] The device includes a horizontal pushing mechanism 100, which is used to push the core 800 to move in the horizontal direction.

[0072] The device includes a dual-valve pressure-holding transfer chamber 200. The dual-valve pressure-holding transfer chamber 200 is used to hold the core sample 800. The method by which the core sample 800 enters the dual-valve pressure-holding transfer chamber 200 can be the same as the entry method in related technologies, and will not be described in detail here.

[0073] Specifically, the dual-valve pressure-holding transfer chamber 200 includes an outer shell assembly 210, a first control valve 220, and a second control valve 230.

[0074] The axial direction of the outer casing assembly 210 is aligned with the horizontal direction, and one end of the outer casing assembly 210 along the horizontal direction is connected to the horizontal pushing mechanism 100. Specifically, the outer casing assembly 210 and the horizontal pushing mechanism 100 can be detachably connected by threads or the like, facilitating installation and disassembly. A sealing design is also employed to facilitate the formation of a high-pressure sealing device. The horizontal pushing mechanism 100 is coaxially arranged with the dual-valve pressure-holding transfer chamber 200.

[0075] The first control valve 220 and the second control valve 230 are respectively sealed to the housing assembly 210. The sealing method can be the same as in related technologies. The first control valve 220 and the second control valve 230 can be located inside the housing assembly 210, and are configured to control the opening or closing of the interior cavity of the housing assembly 210. Specifically, the first control valve 220 and the second control valve 230 can be ball valves or gate valves, etc.

[0076] The first control valve 220 is located on the side of the second control valve 230 facing the horizontal pushing mechanism 100, and the core 800 is located within the outer casing assembly 210 between the first control valve 220 and the second control valve 230. When the first control valve 220 is opened, the inner cavity of the outer casing assembly 210 containing the core 800 communicates with the inner cavity of the horizontal pushing mechanism 100. When the second control valve 230 is opened, the inner cavity of the outer casing assembly 210 containing the core 800 communicates with the inner cavity of the circumferential cutting autoclave 300.

[0077] The apparatus includes a circumferential cutting autoclave 300, which is connected to the other end of the outer casing assembly 210 along its extension direction. The circumferential cutting autoclave 300 is configured to cut core 800. Alternatively, the circumferential cutting autoclave 300 is configured to separate the core tube and cut the core 800. Specifically, the outer casing assembly 210 and the circumferential cutting autoclave 300 can be detachably connected by threads or the like, facilitating installation and disassembly. A sealing design is also employed to facilitate the formation of a high-pressure sealed device.

[0078] It should be noted that when only the core 800 is between the first control valve 220 and the second control valve 230, the circumferential cutting autoclave 300 is configured to cut the core 800. When the core 800 and the core tube 900 wrapped around the outer wall of the core 800 are included between the first control valve 220 and the second control valve 230, the circumferential cutting autoclave 300 is configured to separate the core tube 900 and cut the core 800.

[0079] The device includes a vertical pushing mechanism 400, which is connected to the top of the ring-cutting autoclave 300. The vertical pushing mechanism 400 is used to cut and push the core 800 to move in the vertical direction.

[0080] It should be noted that the vertical pushing mechanism 400 can further cut the core 800 after it has been cut by the ring-cutting autoclave 300, and push the further cut core 800 into the microbial culture chamber.

[0081] The apparatus includes a microbial culture chamber 500, which is connected to the bottom of a ring-cut autoclave 300. The microbial culture chamber 500 is configured to receive the cut core 800.

[0082] The device includes a first pressure regulating mechanism 600, which is configured to regulate the pressure inside the horizontal pushing mechanism 100 and the pressure inside the first control valve 220 in the housing assembly 210 on the side facing the horizontal pushing mechanism 100. In this way, when the first control valve 220 is opened, it is less likely to affect the ambient pressure of the core 800.

[0083] The device includes a second pressure regulating mechanism 700, which is configured to regulate the pressure inside the ring-cutting autoclave 300, the vertical pushing mechanism 400, and the microbial culture chamber 500, as well as the pressure inside the cavity of the second control valve 230 in the outer shell assembly 210 on the side opposite to the horizontal pushing mechanism 100. In this way, when the second control valve 230 is opened, it does not easily affect the environmental pressure of the core 800.

[0084] It should be noted that the internal channels of the horizontal pushing mechanism 100, the dual-valve pressure-holding transfer chamber 200, the vertical pushing mechanism 400, the ring-cutting autoclave 300, and the microbial culture chamber 500 are interconnected to form a high-pressure sealing device. The pressure inside the device can be balanced through the first pressure regulating mechanism 600 and the second pressure regulating mechanism 700.

[0085] For ease of installation and disassembly, the horizontal pushing mechanism 100, the dual-valve pressure-holding transfer chamber 200, the vertical pushing mechanism 400, the ring-cutting autoclave 300, and the microbial culture chamber 500 are detachably connected.

[0086] It is understood that the secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture provided in this application embodiment includes a horizontal pushing mechanism 100, a dual-valve pressure-holding transfer chamber 200, a vertical pushing mechanism 400, a ring-cutting autoclave 300, a microbial culture chamber 500, a first pressure regulating mechanism 600, and a second pressure regulating mechanism 700. The first pressure regulating mechanism 600 ensures that the pressure inside the horizontal pushing mechanism 100 is equal to the pressure inside the chamber of the first control valve 220 in the outer shell assembly 210 facing the horizontal pushing mechanism 100. The second pressure regulating mechanism 700 ensures that the pressure inside the ring-cutting autoclave 300, the vertical pushing mechanism 400, and the microbial culture chamber 500 is equal to the pressure inside the chamber of the second control valve 230 in the outer shell assembly 210 facing away from the horizontal pushing mechanism 100. In this way, the core 800 remains under pressure during the transfer process. The first control valve 220 and the second control valve 230 are then opened. The core 800 is pushed from the dual-valve pressure-holding transfer chamber 200 into the circumferential cutting autoclave 300 via the horizontal pushing mechanism 100. Inside the circumferential cutting autoclave 300, the core 800 is cut using the circumferential cutting autoclave 300 and the vertical pushing mechanism 400. The cut core 800 is then further sent into the microbial culture chamber 500 via the vertical pushing mechanism 400, achieving secondary pressure-holding transfer and propagation cultivation.

[0087] The specific structure of the horizontal pushing mechanism 100 is described below.

[0088] Figure 3 for Figure 2 Cross-sectional view along the AA direction. Figure 4 for Figure 2 Cross-sectional view along the BB direction.

[0089] See Figures 1 to 4 As shown, in some embodiments, the horizontal pushing mechanism 100 includes a first motor 110, a first screw 120, a first push nut 130, a first high-pressure resistant tube 140, a first sleeve 150, and a push rod 160.

[0090] In this design, the drive shaft of the first motor 110 is connected to the first screw 120, which is inserted into the first high-pressure resistant tube 140. A first push nut 130 is fitted onto the first screw 120, and rotation of the first screw 120 causes the first push nut 130 to move horizontally. A first sleeve 150 is mounted on the first screw 120, with one end connected to the first push nut 130 and the other end connected to a push rod 160. A gap exists between the inner wall of the first sleeve 150 and the outer wall of the first screw 120. The first motor 110 can be a servo motor.

[0091] When the horizontal pushing mechanism 100 is running, the first motor 110 drives the first screw 120 to rotate, and the first screw 120 drives the first push nut 130 to move horizontally. The first push nut 130 drives the first sleeve 150 to move horizontally. The first sleeve 150 drives the push rod 160 to move horizontally. The push rod 160 is used to push the core 800 to move horizontally.

[0092] Understandably, by setting the first sleeve 150, with a gap between the inner wall of the first sleeve 150 and the outer wall of the first screw 120, the first push nut 130 drives the first sleeve 150 to move horizontally. The first sleeve 150 then drives the push rod 160 to move horizontally. The push rod 160 is used to push the core 800 to move horizontally. In this way, the length of the first push nut 130 can be reduced, thus reducing transmission resistance.

[0093] The horizontal pushing mechanism 100 includes a first sealing end cap, a first sealing element, and a first bearing.

[0094] The first sealing end cap is located at the end of the first high-pressure resistant tube 140 facing the first motor 110. The first sealing end cap is connected to the first high-pressure resistant tube 140 and is coaxially arranged with the first high-pressure resistant tube 140. The first screw 120 is partially located inside the first sealing end cap, and a first seal and a first bearing are provided between the outer wall of the first screw 120 and the inner wall of the first sealing end cap.

[0095] The horizontal pushing mechanism 100 includes a first retaining ring mounting and connecting sealing joint 170, a first retaining ring 180, a high-pressure pipe mounting and sealing connecting joint 190, and a first sealing connecting joint 1100.

[0096] The first retaining ring 180 is located at the end of the first high-pressure resistant tube 140 opposite to the first motor 110, and the first retaining ring 180 is coaxially arranged with the first high-pressure resistant tube 140. The first retaining ring 180 is mounted on the sealing joint 170 and the first high-pressure resistant tube 140, thereby connecting the first retaining ring 180 with the first high-pressure resistant tube 140.

[0097] The high-pressure pipe mounting and sealing connection joint 190 is located at the end of the first retaining ring 180 mounting and sealing connection joint 170 that is away from the first motor 110. The high-pressure pipe mounting and sealing connection joint 190 and the first retaining ring 180 mounting and sealing connection joint 170 are connected and are coaxially arranged.

[0098] The first sealing connection joint 1100 is located at the end of the high-pressure pipe mounting sealing connection joint 190 opposite to the first motor 110. The high-pressure pipe mounting sealing connection joint 190 and the first sealing connection joint 1100 are connected and coaxially arranged. The first sealing connection joint 1100 is used to connect with the dual-valve pressure-holding transfer chamber 200.

[0099] The specific structure of the dual-valve pressure-holding transfer chamber 200 is described below.

[0100] Figure 5 A schematic diagram showing the open state of the dual-valve pressure-holding transfer chamber in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea pressure-holding cores provided in this application.

[0101] See Figure 1 , Figure 2 and Figure 5 As shown, the outer shell assembly 210 of the dual-valve pressure-holding transfer chamber 200 includes a first pressure-holding transfer chamber sealing connection joint 211, a second pressure-holding transfer sealing connection joint 212, and a connecting pipe 213.

[0102] The first control valve 220 and the second control valve 230 are located between the first pressure-holding transfer chamber sealing connection joint 211 and the second pressure-holding transfer sealing connection joint 212. The first control valve 220, the second control valve 230, the first pressure-holding transfer chamber sealing connection joint 211 and the second pressure-holding transfer sealing connection joint 212 are coaxially arranged.

[0103] The connecting pipe 213 is located between the first control valve 220 and the second control valve 230. The connecting pipe 213 is coaxially arranged with the first control valve 220 and the second control valve 230 respectively. The core 800 and the core tube 900 can be located inside the connecting pipe 213.

[0104] The specific structure of the ring-cutting high-pressure autoclave 300 is described below.

[0105] Figure 6 A schematic diagram of the state of the core tube separated by the high-pressure autoclave in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea cores provided in this application. Figure 7 A schematic diagram of the state of cutting the core in a high-pressure autoclave in a secondary pressure-holding transfer treatment device for microbial culture of deep-sea cores provided in this application.

[0106] See Figure 2 , Figures 5 to 7 As shown, in some embodiments, the circumferential cutting autoclave 300 is provided with a horizontal inner cavity 310 and a vertical inner cavity 320 that are connected at right angles. The horizontal inner cavity 310 is configured to accommodate a portion of the horizontal pushing mechanism 100, and the vertical inner cavity 320 is configured to accommodate a portion of the vertical pushing mechanism 400. Specifically, the horizontal inner cavity 310 and the horizontal pushing mechanism 100 are coaxially arranged.

[0107] The circumferential cutting autoclave 300 includes a circumferential cutting head 330. The circumferential cutting head 330 is located on the side of the horizontal inner cavity 310 facing the vertical inner cavity 320. The cutting cavity 331 of the circumferential cutting head 330 is connected to both the horizontal inner cavity 310 and the vertical inner cavity 320. The diameter of the cutting cavity 331 is smaller than the diameter of the core 800.

[0108] The horizontal pushing mechanism 100 pushes the core 800 horizontally. When the core 800 enters the cutting cavity 331, a portion of its outer periphery is cut. The central region of the core 800 then enters the vertical inner cavity 320 after passing through the cutting cavity 331. In this way, the core 800 can be cut to obtain its central region.

[0109] In some embodiments, the axis of the horizontal inner cavity 310 is collinear with the axis of the outer housing assembly 210. The axis of the cutting cavity 331 is collinear with both the axis of the circumferential cutting head 330 and the axis of the horizontal inner cavity 310. This allows for the cutting of the core 800 and the acquisition of its center.

[0110] In some embodiments, the horizontal inner cavity 310 is provided with an abutment structure 340, which is connected to the wall of the horizontal inner cavity 310. The abutment structure 340 is located on the side of the ring cutting head 330 facing the horizontal pushing mechanism 100. The abutment structure 340 is used to abut against the core tube 900 so as to separate the core tube 900 and the core 800.

[0111] It should be noted that at the position of the abutment structure 340, the diameter of the horizontal inner cavity 310 becomes smaller. The horizontal pushing mechanism 100 pushes horizontally, and the core tube 900, abutting against the abutment structure 340, cannot continue to move horizontally. The horizontal pushing mechanism 100 pushes the core 800 to continue moving horizontally, thereby separating the core 800 from the core tube 900. The horizontal pushing mechanism 100 pushes the core 800 into the cutting cavity 331, and then into the vertical inner cavity 320. The abutment structure 340 can be a right-angled step.

[0112] In some embodiments, the circumferential cutting autoclave 300 is provided with a waste chamber 350, which is located around the circumferential cutting head 330. The waste chamber 350 is connected to the horizontal inner cavity 310, and the diameter of the waste chamber 350 is larger than the diameter of the horizontal inner cavity 310. The waste chamber 350 can accommodate the cut core 800.

[0113] In some embodiments, the circumferential cutting autoclave 300 is provided with an observation window 360, which is located on the side of the vertical inner cavity 320 away from the horizontal inner cavity 310, and is opposite to the circumferential cutting head 330. The observation window 360 is used to observe the cutting process of the core 800.

[0114] Specifically, the high-pressure autoclave 300 includes a high-pressure chamber body 370. The high-pressure chamber body 370 is provided with an observation hole, which covers an observation glass, thus forming an observation window 360. The observation glass is connected to the high-pressure chamber body 370. The observation glass can be sapphire.

[0115] The high-pressure chamber body 370 is provided with a vertical inner cavity 320 and a partial horizontal inner cavity 310.

[0116] The circumferential cutting autoclave 300 includes a flange sealing end cover 380, which is located on the side of the high-pressure chamber body 370 facing the horizontal pushing mechanism 100. The flange sealing end cover 380 is coaxially arranged with the horizontal inner cavity 310 of the high-pressure chamber body 370. The flange sealing end cover 380 is provided with a portion of the horizontal inner cavity 310.

[0117] The circumferential cutting autoclave 300 includes a flange end cap 390 and a third sealing connection joint 3100. The third sealing connection joint 3100 is located on the side of the flange sealing end cap 380 facing the horizontal pushing mechanism 100. The third sealing connection joint 3100 is coaxially arranged with the horizontal inner cavity 310 of the flange sealing end cap 380. The flange sealing end cap 380 has a portion of the horizontal inner cavity 310. The flange end cap 390 is used to connect the third sealing connection joint 3100 and the flange sealing end cap 380.

[0118] In some embodiments, the ring-cutting autoclave 300 is provided with a third control valve 3110, which is disposed in the vertical inner cavity 320 and located at the lower part of the vertical inner cavity 320.

[0119] The third control valve 3110 can be a ball valve or a gate valve, etc. The third control valve 3110 is used to control whether the inner cavity of the circumferential cutting autoclave 300 is connected to or disconnected from the external environment. The third control valve 3110 is also used to control whether the inner cavity of the circumferential cutting autoclave 300 is connected to or disconnected from the inner cavity of the microbial culture chamber 500.

[0120] The specific structure of the vertical push mechanism 400 is described below.

[0121] Figure 8 A schematic diagram of the vertical pushing mechanism cutting the core in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea cores provided in this application. Figure 9 A schematic diagram showing the state of the vertical pushing mechanism driving the core movement in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea cores provided in this application. Figure 10 for Figure 2 Cross-sectional view along the CC direction. Figure 11 for Figure 2 Cross-sectional view along the DD direction.

[0122] See Figure 2 , Figures 8 to 11 As shown, in some embodiments, the vertical pushing mechanism 400 includes a vertical pushing assembly 410 and a pushing cutter head 420. The vertical pushing assembly 410 is connected to the top of the ring-cutting autoclave 300, and a portion of the vertical pushing assembly 410 is located within the vertical inner cavity 320. The pushing cutter head 420 is located within the vertical inner cavity 320 and is connected to the bottom of the vertical pushing assembly 410.

[0123] The vertical pushing assembly 410 is configured to drive the pushing cutter head 420 to move vertically. The pushing cutter head 420 is configured to cut the core 800 entering the vertical inner cavity 320 from the cutting cavity 331 and drive the core 800 to move downwards vertically. Specifically, it can move downwards to the stirring impeller 530 inside the microbial culture chamber 500. The stirring impeller 530 is configured to rotate to dislodge the core from the pushing cutter head 420 and stir the core.

[0124] In some embodiments, the vertical push assembly 410 includes a second motor 415, a second screw 416, a second push nut 417, a second high-pressure resistant tube 418, and a second sleeve 419.

[0125] In this design, the drive shaft of the second motor 415 is connected to the second screw 416, which is inserted into the second high-pressure resistant tube 418. A second push nut 417 is fitted onto the second screw 416, and the rotation of the second screw 416 causes the second push nut 417 to move vertically. A second sleeve 419 is fitted onto the second screw 416, with one end connected to the first push nut 130 and the other end connected to the push cutter head 420. There is a gap between the inner wall of the first sleeve 150 and the outer wall of the first screw 120.

[0126] The second motor 415 can be a servo motor.

[0127] When the vertical pushing assembly 410 is running, the second motor 415 drives the second screw 416 to rotate, and the second screw 416 drives the second push nut 417 to move vertically. The second push nut 417 drives the second sleeve 419 to move horizontally. The second sleeve 419 drives the pushing cutter head 420 to move vertically. The pushing cutter head 420 is used to cut the core 800 and push the core 800 to move vertically.

[0128] Understandably, by setting up a second sleeve 419 with a gap between its inner wall and the outer wall of the second screw 416, the second push nut 417 drives the second sleeve 419 to move vertically. The second sleeve 419 then drives the push cutter head 420 to move vertically. The push cutter head 420 is used to cut the core 800 and push the core 800 to move vertically. This reduces the length of the second push nut 417 and decreases transmission resistance.

[0129] The vertical push assembly 410 includes a second sealing end cap 411, a second seal, and a second bearing.

[0130] The second sealing end cap 411 is located at the end of the second high-pressure resistant tube 418 facing the second motor 415. The second sealing end cap 411 is connected to the second high-pressure resistant tube 418 and is coaxially arranged with it. The second screw 416 is partially located inside the second sealing end cap 411, and a second seal and a second bearing are provided between the outer wall of the second screw 416 and the inner wall of the second sealing end cap 411.

[0131] The vertical push assembly 410 includes a second sealing connection joint 412, a connecting flange 413, and a second retaining ring 414.

[0132] The second sealing connection joint 412 is located at the end of the second high-pressure resistant tube 418 opposite to the second motor 415. The second sealing connection joint 412 is coaxially arranged with and connected to the second high-pressure resistant tube 418. The second sealing connection joint 412 is used to connect to the ring-cutting autoclave 300.

[0133] A connecting flange 413 is fitted onto the outer wall of the second sealing connection joint 412, and the connecting flange 413 is used to connect with the ring-cutting autoclave 300. The connecting flange 413 and the second sealing connection joint 412 are coaxially arranged.

[0134] The second retaining ring 414 is located at the end of the second sealing connection joint 412 opposite to the second motor 415. The second retaining ring 414 and the second sealing connection joint 412 are coaxially arranged. The second retaining ring 414 is sleeved on the second sleeve 419. The second retaining ring 414 is used to connect with the ring-cutting autoclave 300.

[0135] The specific structure of the microbial culture chamber 500 is described below.

[0136] See Figure 2 , Figures 7 to 9 As shown, the microbial culture chamber 500 includes a microbial culture chamber body 510, a fourth control valve 520, a stirring impeller 530, and a third motor 540.

[0137] The fourth control valve 520 is connected to the microbial culture chamber 510, which includes the fourth control valve 520, located at the upper part of the chamber. The fourth control valve 520 controls whether the inner cavity of the microbial culture chamber 510 is connected to or disconnected from the external environment. Alternatively, the fourth control valve 520 controls whether the inner cavity of the microbial culture chamber 510 is connected to or disconnected from the inner cavity of the high-pressure autoclave 300. The fourth control valve 520 can be a ball valve or a gate valve, etc. The inner cavity of the microbial culture chamber 510 can be filled with nutrient solution.

[0138] The stirring impeller 530 and the third motor 540 are located inside the microbial culture chamber 510. The third motor 540 drives the stirring impeller 530 to rotate, thereby stirring the sample and nutrient solution to ensure thorough mixing. The third motor 540 can be a servo motor.

[0139] The specific structure of the first pressure regulating mechanism 600 is described below.

[0140] See Figure 1 and Figure 2 As shown, the first pressure regulating mechanism 600 includes a first water injection valve 610 and a first air release valve 620, both of which are connected to the inner cavity of the horizontal pushing mechanism 100.

[0141] Water is injected into the inner cavity of the horizontal pushing mechanism 100 through the first water injection valve 610, and air is released through the first air release valve 620.

[0142] Specifically, the first water injection valve 610 can be inserted into the first sealing end cap, and the first water injection valve 610 communicates with the inner cavity of the horizontal pushing mechanism 100. The first vent valve 620 can be connected to the high-pressure pipe mounting sealing connection joint 190 through a pipeline. The first vent valve 620 communicates with the inner cavity of the horizontal pushing mechanism 100.

[0143] The specific structure of the second pressure regulating mechanism 700 is described below.

[0144] The second pressure regulating mechanism 700 includes a second water injection valve 710, a first connecting valve 720, a second connecting valve 730, a second exhaust valve 740, and a third exhaust valve 750. The second water injection valve 710 is connected to the vertical inner cavity 320. The first connecting valve 720 is connected to the inner cavity of the microbial culture chamber and the second connecting valve 730. The second connecting valve 730 is connected to the inner cavity of the vertical pushing mechanism 400. The second exhaust valve 740 is connected to the inner cavity of the vertical pushing mechanism 400. The third exhaust valve 750 is connected to the inner cavity of the ring-cutting autoclave and the first exhaust valve 620.

[0145] Specifically, the second water injection valve 710 can be inserted into the circumferential cutting autoclave 300, and the second water injection valve 710 is connected to the vertical inner cavity 320. The first connecting valve 720 can be inserted into the microbial culture chamber 510, and the first connecting valve 720 is connected to the inner cavity of the microbial culture chamber 510. The second connecting valve 730 and the second exhaust valve 740 can be inserted into the second sealing end cap 411. The first connecting valve 720 and the second exhaust valve 740 can be connected through a high-pressure hose 760. The third exhaust valve 750 can be inserted into the autoclave body 370, and the third exhaust valve 750 is connected to the inner cavity of the autoclave body 370. The third exhaust valve 750 and the first exhaust valve 620 can be connected through a pipeline.

[0146] Water is injected through the second water injection valve 710, allowing water to enter the ring-cutting autoclave 300 and the vertical pushing mechanism 400. Water also enters the inner cavity of the microbial culture chamber 500 through the third control valve 3110 and the fourth control valve 520. Gas is discharged through the second exhaust valve 740, and further discharged through the third exhaust valve 750 and the first exhaust valve 620.

[0147] See Figure 1 , Figure 2 and Figure 9 As shown, in some embodiments, to facilitate the removal and replacement of the microbial culture chamber, the circumferential autoclave 300 is provided with a first pressure relief valve 3120, which communicates with the inner cavity of the circumferential autoclave 300 and is located below the third control valve 3110.

[0148] The vertical pushing mechanism 400 is equipped with a second pressure relief valve 430, which is connected to both the first connecting valve 720 and the second connecting valve 730. Specifically, a three-way valve can be used, which is connected to the high-pressure hose 760, the second connecting valve 730, and the second pressure relief valve 430. The high-pressure hose 760 is connected to the first connecting valve 720.

[0149] Figure 12 A schematic diagram of the secondary pressure-holding transfer treatment method for microbial culture of deep-sea pressure-holding cores provided in this application.

[0150] See Figure 12 As shown, this application provides a secondary pressure-holding transfer treatment method for microbial culture of deep-sea pressure-holding cores, which is used in the secondary pressure-holding transfer treatment device for microbial culture of deep-sea pressure-holding cores described in the above embodiments.

[0151] The methods include:

[0152] S101, The pressure in the inner cavity of the horizontal pushing mechanism is made equal to the pressure in the inner cavity of the first control valve in the housing assembly facing the horizontal pushing mechanism by the first pressure regulating mechanism.

[0153] S102. The pressure inside the ring-cut autoclave, the vertical pushing mechanism, and the microbial culture chamber is made equal to the pressure inside the second control valve on the side away from the horizontal pushing mechanism in the outer shell assembly through the second pressure regulating mechanism.

[0154] S103, Open the first control valve and the second control valve;

[0155] S104. The horizontal pushing mechanism pushes the core along the horizontal direction to the circumferential cutting autoclave, where the circumferential cutting autoclave cuts the core.

[0156] S105. The vertical pushing mechanism cuts the core after it has been cut by the high-pressure autoclave. The core cut by the vertical pushing mechanism is then pushed into the microbial culture chamber.

[0157] The specific operational steps of the secondary pressure-holding and transfer treatment method for microbial culture of deep-sea pressure-holding cores provided in this application are described below.

[0158] Figure 13 A schematic diagram of the installation process of the secondary pressure-holding transfer treatment device for microbial culture of deep-sea pressure-holding cores provided in this application.

[0159] a. See also Figure 13 As shown, a high-pressure sealed device is formed by connecting the horizontal pushing mechanism 100, the dual-valve pressure-holding transfer chamber 200, the vertical pushing mechanism 400, the ring-cutting autoclave 300, and the microbial culture chamber 500, creating interconnected internal channels. The first control valve 220 and the second control valve 230 of the dual-valve pressure-holding transfer chamber 200 are in a closed, sealed state, and the core 800 and core tube 900 are stored under high pressure within the enclosed space of the dual-valve pressure-holding transfer chamber 200.

[0160] b. Water is injected from the first water injection valve 610 of the horizontal pushing mechanism 100, and the first vent valve 620 vents air. After the air is vented, the first vent valve 620 is closed, and the first water injection valve 610 continues to pressurize and inject water until the pressure in the space connected between the horizontal pushing mechanism 100 and the first control valve 220 of the dual-valve pressure-holding transfer chamber 200 is equal to the pressure in the closed space inside the dual-valve pressure-holding transfer chamber 200.

[0161] c. Connect the first connecting valve 720 and the second connecting valve 730 through the high-pressure hose 760. Open the first connecting valve 720 and the second connecting valve 730 to the open state. Close the second pressure relief valve 430. The third control valve 3110 and the fourth control valve 520 are in the open state. Water is injected from the second water injection valve 710 and air is released from the first air release valve 620 and the second air release valve 740. After the water is full, close the first air release valve 620 and the second air release valve 740. Continue to inject water from the second water injection valve 710 to realize water injection and pressurization of the closed space connected to the dual-valve pressure-holding transfer chamber 200, the vertical pushing mechanism 400, the ring-cutting autoclave 300, and the microbial culture chamber 500 until the internal space pressure is balanced with the pressure of the closed space inside the dual-valve pressure-holding transfer chamber 200. Then close the second water injection valve 710.

[0162] d. Open the first control valve 220 and the second control valve 230 of the dual-valve pressure-holding transfer chamber 200. The push rod 160 in the horizontal pushing mechanism 100 moves forward to push the core 800 and core tube 900 in the dual-valve pressure-holding transfer chamber 200 into the ring-cutting high-pressure autoclave 300. The core tube 900 stops sliding after contacting the abutting structure 340 of the high-pressure chamber body 370.

[0163] e. The drive push rod 160 continues to move forward, pushing the core 800 to overcome the friction of the core tube 900 and continue to slide forward relative to it. Further, the core 800 comes into contact with and is squeezed by the circumferential cutting head 330. The central part of the core 800 is circumferentially cut into the cutting cavity 331 of the circumferential cutting head 330 and continues to move forward. The remaining peripheral part of the core 800 enters the waste chamber 350. Further, the central part of the core 800 moves forward into the vertical inner cavity 320 of the high-pressure chamber body 370 and reaches the designed length range. Then, the vertical pushing component 410 in the vertical pushing mechanism 400 drives... The pusher head 420 moves forward to shear the core 800 in the center of the vertical inner cavity 320 of the high-pressure chamber body 370, forming a sample that can be cultured by microorganisms. The pusher head 420 continues to move forward to send the sample into the stirring impeller 530 of the microbial culture chamber 500. The stirring impeller 530 rotates to stir the sample and fully mix it with the nutrient solution in the microbial culture chamber 500. If more samples need to be pushed into the microbial culture chamber 500, the vertical pushing component 410 in the vertical pushing mechanism 400 retrieves the pusher head 420, and step e is repeated.

[0164] f. In the vertical pushing mechanism 400, the vertical pushing component 410 retracts the pushing head 420, closes the third control valve 3110 and the fourth control valve 520, opens the first pressure relief valve 3120, and after releasing the high-pressure area between the third control valve 3110 and the fourth control valve 520, further closes the second connecting valve 730 and the first connecting valve 720, and opens the second pressure relief valve 430 to release the high-pressure area of ​​the connecting hose between the second connecting valve 730 and the first connecting valve 720. Then, the connection between the microbial culture chamber 500 and the ring-cut autoclave 300 can be disassembled, and the microorganisms in the core 800 can be further pressurized and transferred to other locations.

[0165] g. Further, if it is necessary to store more samples in other identical microbial culture chambers 500, the microbial culture chamber 500 is sealed to the circumferential autoclave 300. The fourth control valve 520 is opened, the first connecting valve 720 is opened, the high-pressure hose 760 connecting the first connecting valve 720 and the second connecting valve 730 is opened, the second pressure relief valve 430 and the first pressure relief valve 3120 are opened, and water is injected from the third water injection valve 770. After venting, the first pressure relief valve 3120 and the second pressure relief valve 430 are closed. The pressure is increased until the internal pressure of the newly connected microbial culture chamber 500 is balanced with the internal pressure of the circumferential autoclave 300. Then, the third control valve 3110 and the fourth control valve 520 are opened, and step e is repeated to achieve repeated sampling. If it is necessary to disassemble the newly connected microbial culture chamber 500, step f can be followed.

[0166] Finally, it should be noted that other embodiments of the invention will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This invention is intended to cover any variations, uses, or adaptations of the invention that follow the general principles of the invention and include common knowledge or customary techniques in the art not disclosed herein, and is not limited to the precise structures described above and shown in the accompanying drawings, and various modifications and changes can be made without departing from its scope. The scope of the invention is limited only by the appended claims.

Claims

1. A secondary pressure-holding transfer processing device suitable for microbial culture of deep-sea pressure-holding core samples, characterized in that, include: A horizontal pushing mechanism (100) is used to push the core along the horizontal direction; A dual-valve pressure-holding transfer chamber (200) includes an outer shell assembly (210), a first control valve (220), and a second control valve (230). One end of the outer shell assembly (210) in the horizontal direction is connected to the horizontal pushing mechanism (100). The first control valve (220) and the second control valve (230) are respectively sealed to the outer shell assembly (210). The first control valve (220) and the second control valve (230) are configured to control the opening or closing of the inner cavity of the outer shell assembly (210). The first control valve (220) is located on the side of the second control valve (230) facing the horizontal pushing mechanism (100). The core is located inside the outer shell assembly (210) between the first control valve (220) and the second control valve (230). A circumferential cutting autoclave (300) is connected to the other end of the outer shell assembly (210), and the circumferential cutting autoclave (300) is configured to cut the core. A vertical pushing mechanism (400) is connected to the top of the ring-cutting autoclave (300), and the vertical pushing mechanism (400) is used to cut and push the core to move in the vertical direction; A microbial culture chamber (500) is connected to the bottom of the ring-cut autoclave (300), and the microbial culture chamber (500) is configured to receive the cut core. The first pressure regulating mechanism (600) is configured to regulate the pressure of the inner cavity of the horizontal pushing mechanism (100) and the pressure of the inner cavity of the first control valve (220) in the housing assembly (210) on the side facing the horizontal pushing mechanism (100); The second pressure regulating mechanism (700) is configured to regulate the pressure of the inner cavity of the ring-cut autoclave (300), the vertical pushing mechanism (400), the microbial culture chamber (500), and the pressure of the inner cavity of the second control valve (230) in the outer shell assembly (210) on the side opposite to the horizontal pushing mechanism (100).

2. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to claim 1, characterized in that, The ring-cutting autoclave (300) is provided with a horizontal inner cavity (310) and a vertical inner cavity (320) that are connected at right angles. The horizontal inner cavity (310) is configured to accommodate a horizontal pushing mechanism (100), and the vertical inner cavity (320) is configured to accommodate a vertical pushing mechanism (400). The circumferential cutting autoclave (300) includes a circumferential cutting head (330), which is disposed on the side of the horizontal inner cavity (310) facing the vertical inner cavity (320). The cutting cavity (331) of the circumferential cutting head (330) is connected to the horizontal inner cavity (310) and the vertical inner cavity (320) respectively. The diameter of the cutting cavity (331) is smaller than the diameter of the core.

3. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to claim 2, characterized in that, The axis of the horizontal inner cavity (310) is collinear with the axis of the outer shell assembly (210); The axis of the cutting cavity (331) is collinear with the axis of the circumferential cutting head (330) and the axis of the horizontal inner cavity (310), respectively.

4. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to claim 2, characterized in that, The horizontal inner cavity (310) is provided with an abutment structure (340), which is connected to the wall of the horizontal inner cavity (310). The abutment structure (340) is located on the side of the ring cutting head (330) facing the horizontal pushing mechanism (100). The abutment structure (340) is used to abut against the core tube to separate the core tube from the core.

5. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to claim 2, characterized in that, The high-pressure autoclave (300) is provided with a waste chamber (350), which is located around the periphery of the circumferential cutting head (330). The waste chamber (350) is connected to the horizontal inner cavity (310), and the diameter of the waste chamber (350) is larger than the diameter of the horizontal inner cavity (310).

6. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to claim 2, characterized in that, The vertical pushing mechanism (400) includes a vertical pushing assembly (410) and a pushing cutter head (420). The vertical pushing assembly (410) is connected to the top of the ring-cutting autoclave (300). The vertical pushing assembly (410) is partially located within the vertical inner cavity (320). The pushing cutter head (420) is located within the vertical inner cavity (320) and is connected to the bottom of the vertical pushing assembly (410). The vertical pushing component (410) is configured to drive the pushing cutter head (420) to move in a vertical direction. The pushing cutter head (420) is configured to cut the core entering the vertical inner cavity (320) from the cutting cavity (331) and drive the core to move downward in a vertical direction to the stirring impeller (530) in the microbial culture chamber (500). The stirring impeller (530) is configured to cause the core to fall off the pushing cutter head (420) and stir the core by rotating.

7. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to claim 2, characterized in that, The circumferential cutting autoclave (300) is provided with an observation window (360), which is located on the side of the vertical inner cavity (320) away from the horizontal inner cavity (310) and is opposite to the circumferential cutting head (330).

8. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to any one of claims 2 to 7, characterized in that, The first pressure regulating mechanism (600) includes a first water injection valve (610) and a first air release valve (620), both of which are connected to the inner cavity of the horizontal pushing mechanism (100). The second pressure regulating mechanism (700) includes a second water injection valve (710), a first connecting valve (720), a second connecting valve (730), a second exhaust valve (740), and a third exhaust valve (750). The second water injection valve (710) is connected to the vertical inner cavity (320). The first connecting valve (720) is connected to the inner cavity of the microbial culture chamber (500) and the second connecting valve (730). The second connecting valve (730) is connected to the inner cavity of the vertical pushing mechanism (400). The second exhaust valve (740) is connected to the inner cavity of the vertical pushing mechanism (400). The third exhaust valve (750) is connected to the inner cavity of the ring-cutting autoclave (300) and the first exhaust valve (620).

9. The secondary pressure-holding transfer processing device for deep-sea pressure-holding core microbial culture according to claim 8, characterized in that, The high-pressure reactor (300) is equipped with a third control valve (3110), which is located in the vertical inner cavity (320) and at the lower part of the vertical inner cavity (320); the second water injection valve (710) is located above the third control valve (3110); The second pressure regulating mechanism (700) also includes a third water injection valve (770), which is connected to the vertical inner cavity (320) and is located below the third control valve (3110); The circumferential cutting high-pressure vessel (300) is equipped with a first pressure relief valve (3120), which is connected to the inner cavity of the circumferential cutting high-pressure vessel (300). The first pressure relief valve (3120) is located below the third control valve (3110). The vertical pushing mechanism (400) is provided with a second pressure relief valve (430), which is connected to the first connecting valve (720) and the second connecting valve (730) respectively; The microbial culture chamber (500) includes a fourth control valve (520), which controls whether the inner cavity of the microbial culture chamber (500) is connected to or not connected to the vertical inner cavity (320).

10. A secondary pressure-holding transfer treatment method suitable for microbial culture of deep-sea pressure-holding core samples, characterized in that, The secondary pressure-holding transfer processing device for microbial culture of deep-sea pressure-holding cores as described in any one of claims 1 to 9; The methods include: The pressure in the inner cavity of the horizontal pushing mechanism is made equal to the pressure in the inner cavity of the first control valve in the housing assembly facing the horizontal pushing mechanism by the first pressure regulating mechanism. The pressure inside the ring-cut autoclave, the vertical pushing mechanism, and the microbial culture chamber is equalized by the pressure inside the chamber of the outer shell assembly on the side of the second control valve away from the horizontal pushing mechanism through the second pressure regulating mechanism. Open the first control valve and the second control valve; The horizontal pushing mechanism pushes the core along the horizontal direction to the circumferential cutting autoclave, where the circumferential cutting autoclave cuts the core. The vertical pushing mechanism cuts the core sample after it has been cut by the circumferential autoclave, and the core sample cut by the vertical pushing mechanism is pushed into the microbial culture chamber by the vertical pushing mechanism.

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