A cell biology-based experimental sample incubator

Abstract

This invention discloses an experimental sample culture device based on cell biology, relating to the field of culture dish technology. It includes a culture section, with a supplementary lighting support at the bottom for providing supplementary lighting to the culture section. A culture medium pusher is mounted on the supplementary lighting support and connected to the bottom of the culture section. Delayed limiting components are mounted on both sides of the supplementary lighting support to stop the culture section. A cover fastener is mounted on the culture section. The use of the culture medium pusher in conjunction with the height-adjustable culture section allows for rapid isolation of the culture medium when observing cultured cell tissues within the culture dish, thus solving the problem that current culture devices are not convenient for rapid culture medium separation and that manual observation time is difficult to control during microscopic observation.

Description

Technical Field

[0001] This invention relates to the field of petri dish technology, specifically to an experimental sample culture device based on cell biology. Background Technology

[0002] Culture dishes are indispensable basic tools in cell biology experiments. Their importance lies in providing a standardized, controllable, and easily observable growth environment for cells. They not only support cell adhesion and proliferation but are also widely used in various experimental scenarios such as transfection, drug treatment, and toxicity testing. Culture dishes also facilitate microscopic observation and direct data recording. However, current culture dishes require the removal of culture medium before microscopic observation, otherwise it can affect microscopic observation. Manual pipetting is cumbersome, can easily damage cells, and increases the risk of cross-infection. Furthermore, it is difficult to quickly separate the culture medium, and manual observation time is difficult to control. If cell observation time is too long, the cells may deteriorate due to decreased ambient temperature and prolonged lack of culture medium nourishment, leading to inaccurate experimental results. Additionally, it is difficult to separate condensate. Traditionally, when opening the protective lid of the culture dish manually, condensate can easily drip onto the cultured cell tissue area, affecting the accuracy of cell experiments.

[0003] Therefore, this invention proposes an experimental sample culture device based on cell biology. Summary of the Invention

[0004] The purpose of this invention is to provide an experimental sample culture device based on cell biology, so as to solve the problems mentioned in the background art that current culture devices are not convenient for rapid separation of culture medium and that it is difficult to control the observation time when performing microscopic observation manually.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an experimental sample culture device based on cell biology, comprising a culture section, wherein a supplementary lighting support is installed at the bottom of the culture section for supplementing light to the culture section; a culture medium pusher is installed on the supplementary lighting support; the culture medium pusher is connected to the bottom of the culture section; delay limiting components are respectively installed on both sides of the supplementary lighting support; the delay limiting components are used to stop the culture section; a cover fastener is installed on the culture section; the culture section includes: a culture dish and a flow guide hole, wherein a ring of through holes is formed at the bottom of the culture dish; a ring of flow guide holes is formed at the upper edge of the culture dish; and the central area of ​​the culture dish is used for culturing cell tissues.

[0006] Preferably, the culture section further includes a light-diffusing plate, which is fixedly installed at the bottom of the culture dish.

[0007] Preferably, the supplementary lighting support includes: a support shell, rubber blocks, a rubber sleeve, and a fixed threaded cylinder. The support shell is fitted onto the bottom of the culture dish; rubber blocks are fixedly installed on both sides of the support shell; four through holes are provided at the bottom of the support shell; a fixed threaded cylinder is fixedly installed on the inner side of the support shell; a rubber sleeve is embedded in the inner side of the support shell, and the outer side of the culture dish is attached to the rubber sleeve.

[0008] Preferably, the supplemental lighting support further includes: a torsion spring, a connecting column, and supplemental lights. The torsion spring is located outside the fixed threaded cylinder; one end of the torsion spring is fixedly connected to the bottom of the culture dish, and the other end of the torsion spring is fixedly connected to the support shell; the connecting column is threadedly connected to the fixed threaded cylinder; the top of the connecting column is fixedly installed at the bottom of the culture dish; three rings of supplemental lights are fixedly installed on the connecting column; the light-diffusing plate is located above the supplemental lights; the light-diffusing plate is located inside the connecting column; and the torsion spring is used to control the rotation and reset of the culture dish.

[0009] Preferably, the supplementary lighting support further includes: a protrusion and a delayed power switch. The delayed power switch is fixedly installed on the support shell, and the end of the delayed power switch is pressed against the bottom of the connecting column. The bottom of the connecting column is fixedly installed with a protrusion, and the protrusion is used to press the delayed power switch. The protrusion has an arc-shaped structure, and the protrusion and the delayed power switch are misaligned.

[0010] Preferably, the culture medium pusher includes: a fixing ring and a sealing shell, wherein the fixing ring is fixedly installed inside the supporting shell by bolts, and the inner and outer rings of the fixing ring are respectively provided with sealing rings; the sealing shell is fixedly installed at the bottom of the culture dish, and a through hole is formed on the sealing shell; the sealing shell is sleeved on the outside of the fixing ring; and the culture medium solution is accumulated inside the sealing shell.

[0011] Preferably, the delay limiting component includes: an electromagnet and a limiting block; two electromagnets are fixedly installed on the support shell; two limiting blocks are slidably inserted into the support shell, and the two limiting blocks pass through the support shell respectively; the limiting blocks are used to stop the culture dish; the ends of the limiting blocks are arc-shaped.

[0012] Preferably, the delay limiting component further includes: a tension spring, with tension springs fixedly connected to each of the two limiting blocks, and the ends of the tension springs fixedly connected to the inner side of the support shell; the electromagnet and the limiting block on the same side are aligned; and the delay power switch and the two electromagnets are connected in series with a battery power supply.

[0013] Preferably, the cover fastener includes: a protective cover and an outer edge ring, the top of the protective cover having a sloping structure; the outer edge ring is fixedly installed on the outside of the protective cover, and the outer edge ring stops and fits against the upper edge of the culture dish; the top of the protective cover is provided with a through hole.

[0014] Preferably, the cover fastener further includes: a liquid collecting ring and a conduit groove. The liquid collecting ring is fixedly installed at the bottom of the protective cover, and a rubber ring is provided on the outside of the liquid collecting ring. The rubber ring on the outside of the liquid collecting ring is attached to the inside of the petri dish. A groove is provided on the inside of the liquid collecting ring. The liquid collecting ring is used to collect condensate. A conduit groove is provided on the outside of the liquid collecting ring. A gap is provided between the outside of the liquid collecting ring and the petri dish.

[0015] Compared with the prior art, the beneficial effects of the present invention are: This invention employs a culture medium pusher combined with an adjustable culture section, allowing staff to quickly isolate the culture medium when observing cells and tissues in the culture dish. The culture medium refracts light, interfering with microscopic observation. This structure also eliminates the need for manual aspiration of the culture medium, reducing the risk of cross-contamination. The use of a time-delay limiting device automatically controls the isolation time of the culture medium, prompting timely cessation of microscopic observation. This ensures cell culture quality, maintains cell physiological homeostasis, improves the reliability of experimental data and long-term cell viability, and prevents cells from being exposed to air for extended periods, avoiding the increased risk of cross-contamination due to excessively low temperatures. Prolonged lack of culture medium protection can affect cell growth quality and interfere with experimental results.

[0016] The cover fasteners can easily shield the culture dish for protection, while the collection ring can easily collect condensate and prevent it from dripping onto the cultured cells. With the help of the conduit and drainage hole, the condensate can be drained directly when the protective cover is removed, so that it can be collected again when the culture is placed in an incubator or other culture environment. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of an experimental sample culture device based on cell biology according to the present invention; Figure 2 This is a cross-sectional view of the internal structure of an experimental sample culture device based on cell biology according to the present invention. Figure 3 This is a schematic diagram of the culture section structure of the present invention; Figure 4 This is a schematic diagram of the inner structure of the petri dish of the present invention; Figure 5 This is a schematic diagram of the supplementary lighting support structure of the present invention; Figure 6 This is a cross-sectional view of the installation position of the time-delayed energizing switch of the present invention; Figure 7 For the present invention Figure 6 Enlarged view of the structure of region B in the middle; Figure 8 This is a schematic diagram of the delay limiting component structure of the present invention; Figure 9 This is a schematic diagram of the cover fastener structure of the present invention.

[0018] In the diagram: 1. Culture section; 101. Petri dish; 1011. Drainage hole; 102. Light distribution plate; 2. Supplemental lighting support; 201. Support shell; 2011. Rubber block; 2012. Rubber sleeve; 202. Fixed threaded cylinder; 203. Torsion spring; 204. Connecting column; 2041. Protrusion; 205. Delayed power switch; 206. Supplemental light; 3. Culture medium pusher; 301. Fixing ring; 302. Sealing shell; 4. Delayed limiting component; 401. Electromagnet; 402. Limiting insert; 403. Tension spring; 5. Cover fastener; 501. Protective cover; 5011. Outer edge ring; 502. Liquid collection ring; 5021. Merging groove. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Example 1: Please refer to Figures 1 to 9 As shown: This invention provides a technical solution: a cell biology-based experimental sample culture device, comprising a culture section 1, a supplementary lighting support 2 installed at the bottom of the culture section 1 for supplementing light to the culture section 1; a culture medium pusher 3 installed on the supplementary lighting support 2; the culture medium pusher 3 connected to the bottom of the culture section 1; delay limiting members 4 respectively installed on both sides of the supplementary lighting support 2 for stopping the culture section 1; a cover fastener 5 installed on the culture section 1; the culture section 1 includes: a culture dish 101 and a flow guide hole 1011, a ring of through holes is formed at the bottom of the culture dish 101; a ring of flow guide holes 1011 is formed at the upper edge of the culture dish 101; the middle area of ​​the culture dish 101 is used for culturing cell tissues.

[0021] The culture section 1 further includes: a light-diffusing plate 102, which is fixedly installed at the bottom of the culture dish 101; the supplementary lighting support 2 includes: a support shell 201, rubber blocks 2011, rubber sleeves 2012, and a fixed threaded cylinder 202, with the support shell 201 fitted onto the bottom of the culture dish 101; rubber blocks 2011 are fixedly installed on both sides of the support shell 201; the bottom of the support shell 201 has four through holes; a fixed threaded cylinder 202 is fixedly installed inside the support shell 201; and a rubber sleeve 2012 is embedded inside the support shell 201. The outer side of the petri dish 101 is attached to a rubber sleeve 2012; the supplementary lighting support 2 also includes: a torsion spring 203, a connecting post 204, and supplementary lights 206. The torsion spring 203 is located outside the fixed threaded cylinder 202; one end of the torsion spring 203 is fixedly connected to the bottom of the petri dish 101, and the other end of the torsion spring 203 is fixedly connected to the support shell 201; the connecting post 204 is threadedly connected to the fixed threaded cylinder 202; the top of the connecting post 204 is fixedly installed on the bottom of the petri dish 101; three rings of supplementary lights 206 are fixedly installed on the connecting post 204; a light-diffusing plate 10 2 is located above the supplementary light 206; the light-diffusing plate 102 is located inside the connecting column 204; the torsion spring 203 is used to control the rotation and reset of the culture dish 101; the culture medium pusher 3 includes: a fixing ring 301 and a sealing shell 302, the fixing ring 301 is fixedly installed inside the support shell 201 by bolts, and the inner and outer rings of the fixing ring 301 are respectively provided with sealing rings; the sealing shell 302 is fixedly installed at the bottom of the culture dish 101, and a through hole is opened on the sealing shell 302; the sealing shell 302 is sleeved on the outside of the fixing ring 301; the sealing shell 302 The internal culture medium solution is stored; the culture medium pusher 3, combined with the height-adjustable culture section 1, allows staff to quickly isolate the culture medium when they need to observe the cells and tissues in the culture dish 101. If there are tiny bubbles, protein precipitates, or serum exudates in the culture medium, they can easily be misjudged as contaminant particles or cell debris under high magnification, increasing the risk of misreading. At the same time, the culture medium will refract light and interfere with microscope observation. This structure also eliminates the need for manual aspiration of the culture medium, reducing the risk of cross-infection. The separation method of this structure will not damage the cells and tissues.

[0022] The supplementary lighting support 2 also includes: a protrusion 2041 and a time-delay switch 205. The time-delay switch 205 is fixedly installed on the support shell 201, and the end of the time-delay switch 205 is pressed against the bottom of the connecting column 204. An NKPZ-22 type time-delay switch 205 can be used. The bottom of the connecting column 204 is fixedly installed with a protrusion 2041, and the protrusion 2041 is used to press against the time-delay switch 205. The protrusion 2041 has an arc-shaped structure, and the protrusion 2041 and the time-delay switch 205 are staggered. The time-delay limiting component 4 includes: an electromagnet 401. Two electromagnets 401 are fixedly installed on the support shell 201, and two limiting blocks 402 are slidably inserted into the support shell 201, with each limiting block 402 passing through the support shell 201; the limiting blocks 402 are used to stop the culture dish 101; the ends of the limiting blocks 402 are arc-shaped; the delay limiting component 4 also includes: a tension spring 403, with tension springs 403 fixedly connected to each of the two limiting blocks 402, and the ends of the tension springs 403 being fixedly connected to the inner side of the support shell 201; the electromagnets 401 and the limiting blocks 402 on the same side are aligned; A delayed-on switch 205 and two electromagnets 401 are connected in series with a battery power supply. The delayed-on limiter 4 automatically controls the isolation time of the culture medium, prompting timely cessation of microscopic observation. This ensures cell culture quality, maintains cell homeostasis, improves the reliability of experimental data and long-term cell viability, prevents cells from being exposed to air for extended periods, and avoids the risk of cross-contamination due to excessively low temperatures. Furthermore, prolonged lack of culture medium protection can affect cell growth quality and interfere with experimental results. This structure is simple to control and can automatically time the process. The spiral moves upwards in the culture dish 101. As the connecting post 204 rotates, it will cause the protrusion 2041 to rotate and squeeze the delayed power-on switch 205 to perform delayed power-on control. During the process, the staff needs to perform cell microscopic examination and take pictures for record. After the delay time is reached, the delayed power-on switch 205 can control the electromagnet 401 to be energized, and the magnetic suction limit plug 402 will retract. At this time, the limit plug 402 can release the stop on the culture dish 101. When the culture dish 101 is not limited, under the elastic torque of the torsion spring 203, the culture dish 101 can drive the connecting post 204 to spiral down and reset.

[0023] In Example 2, based on Example 1, the cover fastener 5 includes: a protective cover 501 and an outer edge ring 5011. The top of the protective cover 501 has a sloping structure. The outer edge ring 5011 is fixedly installed on the outside of the protective cover 501, and the outer edge ring 5011 stops and fits against the upper edge of the culture dish 101. The top of the protective cover 501 has a through hole. The cover fastener 5 also includes: a liquid collecting ring 502 and a channel 5021. The liquid collecting ring 502 is fixedly installed on the bottom of the protective cover 501, and a rubber ring is provided on the outside of the liquid collecting ring 502. The rubber ring on the outside of the liquid collecting ring 502 is attached to the inside of the culture dish 101. A groove is provided on the inside of the liquid collecting ring 502. The liquid collecting ring 502 is used to collect condensate. Water; a conduit 5021 is provided on the outer side of the collection ring 502; a gap is provided between the outer side of the collection ring 502 and the culture dish 101 to facilitate the drainage of condensate; the cover fastener 5 can be used to cover the culture dish 101 for protection, and the collection ring 502 can be used to collect condensate, preventing condensate from dripping onto the cultured cell tissue, reducing condensate residue and further reducing its interference with experimental results. With the conduit 5021 and the guide hole 1011, condensate can be directly drained when the protective cover 501 is removed, so that condensate can be collected again when the culture is placed in an incubator or other culture environment, increasing the accuracy of experimental results.

[0024] The working principle of this embodiment: The rubber block 2011 facilitates manual handling of the culture dish 101. After the culture medium is placed in the culture dish 101, cell culture is carried out in the middle of the culture dish 101. Cell tissues are cultured in a suitable temperature environment. When microscopic cell observation is required, supplemental lighting can be provided by turning on the supplemental light 206. The support shell 201 is placed under a microscope, and the culture dish 101 is manually rotated. At this time, the connecting column 204 is driven to move spirally upward on the fixed threaded cylinder 202, which affects the torsion spring 203. When a torsional torque is applied, as the culture dish 101 moves upward and no longer obstructs the limiting block 402, the limiting block 402 can extend under the pull of the tension spring 403 and stop at the bottom of the culture dish 101. At this time, as the culture dish 101 moves upward, the sealing shell 302 moves upward outside the fixing ring 301, and the volume of the cavity between the sealing shell 302 and the fixing ring 301 increases. The culture medium can flow directly into the sealing shell 302 from a ring of holes at the bottom of the culture dish 101 for temporary storage, which makes it easier to observe under a microscope. As the culture dish 101 moves upward, the rotating connecting column 204 causes the protrusion 2041 to rotate and press the delayed energizing switch 205, thus controlling the delayed energizing. During this process, staff need to perform timely cell microscopic examinations and take photos. After the delay time is reached, the delayed energizing switch 205 controls the electromagnet 401 to energize, causing the magnetic limiting plug 402 to retract and the tension spring 403 to stretch. At this point, the limiting plug 402 can release its stop on the culture dish 101. Now that the culture dish 101 is no longer limited, under the elastic torque of the torsion spring 203, the culture dish 101 can drive the connecting column 204 to spiral downward and reset. At this time, the sealing shell 302 also moves downward, and the culture medium inside the sealing shell 302 can be squeezed out from the through hole at the bottom of the culture dish 101 to re-infiltrate the cultured cells. When microscopic observation is stopped, the sample is placed in an incubator for incubation to avoid excessive temperature drop. At this time, as the connecting column 204 rotates and resets, it will drive the protrusion 2041 to rotate and squeeze the delayed energizing switch 205, thereby controlling the electromagnet 401 to cut off the power. As the temperature difference changes, the condensate will flow from the inclined protective cover 501 to the collection ring 502 for collection, preventing it from dripping directly into the culture dish 101. When the protective cover 501 is pulled out, the collection ring 502 can be moved upward together. When the confluence groove 5021 moves to the guide hole 1011, because the collection ring 502 has a rubber ring on the outside to prevent water leakage, the condensate will flow out from the confluence groove 5021 through the connection of the confluence groove 5021 and then be discharged from the guide hole 1011, thereby draining the condensate and emptying the collection ring 502.

[0025] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0026] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell biology-based experimental sample culture device, comprising a culture section (1), wherein a supplementary lighting support (2) is installed at the bottom of the culture section (1), characterized in that: The supplementary lighting support (2) is used to supplement the light to the culture section (1); a culture medium pusher (3) is installed on the supplementary lighting support (2); the culture medium pusher (3) is connected to the bottom of the culture section (1); The supplementary lighting support (2) is equipped with delay limiting components (4) on both sides; the delay limiting components (4) are used to stop the culture section (1); the culture section (1) is equipped with a cover fastener (5); The culture section (1) includes: a culture dish (101), the bottom of which has a ring of through holes; and the upper edge of the culture dish (101) has a ring of flow guide holes (1011).

2. The cell biology-based experimental sample culture device according to claim 1, characterized in that: The culture section (1) further includes a light-diffusing plate (102), which is fixedly installed at the bottom of the culture dish (101).

3. The cell biology-based experimental sample culture device according to claim 2, characterized in that: The supplementary lighting support (2) includes: a support shell (201), the bottom of the culture dish (101) is fitted with the support shell (201); rubber blocks (2011) are fixedly installed on both sides of the support shell (201); the bottom of the support shell (201) is provided with four through holes; a fixed threaded cylinder (202) is fixedly installed on the inner side of the support shell (201); a rubber sleeve (2012) is embedded on the inner side of the support shell (201), and the outer side of the culture dish (101) is attached to the rubber sleeve (2012).

4. The cell biology-based experimental sample culture device according to claim 3, characterized in that: The supplementary lighting support (2) further includes: a torsion spring (203) and a connecting column (204). The torsion spring (203) is located outside the fixed threaded cylinder (202). One end of the torsion spring (203) is fixedly connected to the bottom of the petri dish (101), and the other end of the torsion spring (203) is fixedly connected to the support shell (201). The connecting column (204) is threadedly connected to the fixed threaded cylinder (202). The top of the connecting column (204) is fixedly installed at the bottom of the petri dish (101). Three rings of supplementary lights (206) are fixedly installed on the connecting column (204). The light-diffusing plate (102) is located above the supplementary lights (206). The light-diffusing plate (102) is located inside the connecting column (204).

5. The cell biology-based experimental sample culture device according to claim 4, characterized in that: The supplementary lighting support (2) further includes: a protrusion (2041), a time-delay power switch (205) is fixedly installed on the support shell (201), and the end of the time-delay power switch (205) is pressed against the bottom of the connecting column (204); the bottom of the connecting column (204) is fixedly installed with a protrusion (2041), and the protrusion (2041) is used to press the time-delay power switch (205).

6. The cell biology-based experimental sample culture device according to claim 3, characterized in that: The culture medium pusher (3) includes: a fixing ring (301), which is fixedly installed inside the support shell (201) by bolts, and the inner and outer rings of the fixing ring (301) are respectively provided with sealing rings; a sealing shell (302) is fixedly installed at the bottom of the culture dish (101), and a ring of through holes is opened on the sealing shell (302); the sealing shell (302) is sleeved on the outside of the fixing ring (301); and the culture medium solution is accumulated inside the sealing shell (302).

7. The cell biology-based experimental sample culture device according to claim 5, characterized in that: The delay limiting component (4) includes: an electromagnet (401), two electromagnets (401) are fixedly installed on the support shell (201); two limiting blocks (402) are slidably inserted into the support shell (201), and the two limiting blocks (402) pass through the support shell (201) respectively; the limiting blocks (402) are used to stop the culture dish (101).

8. The cell biology-based experimental sample culture device according to claim 7, characterized in that: The delay limiting component (4) further includes: a tension spring (403), with tension springs (403) fixedly connected to the two limiting blocks (402) respectively, and the ends of the tension springs (403) fixedly connected to the inner side of the support shell (201); the electromagnet (401) and the limiting block (402) on the same side are aligned.

9. The cell biology-based experimental sample culture device according to claim 1, characterized in that: The cover fastener (5) includes: a protective cover (501), the top of which is a sloping structure; an outer edge ring (5011) is fixedly installed on the outside of the protective cover (501), and the outer edge ring (5011) stops and fits against the upper edge of the culture dish (101); the top of the protective cover (501) is provided with a through hole.

10. The cell biology-based experimental sample culture device according to claim 9, characterized in that: The cover fastener (5) further includes: a liquid collection ring (502), the liquid collection ring (502) is fixedly installed at the bottom of the protective cover (501), and a rubber ring is provided on the outside of the liquid collection ring (502), the rubber ring on the outside of the liquid collection ring (502) is attached to the inside of the petri dish (101); a groove is provided on the inside of the liquid collection ring (502); a confluence groove (5021) is opened on the outside of the liquid collection ring (502); a gap is provided between the outside of the liquid collection ring (502) and the petri dish (101).

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