A medicine bacteriostatic detection device and method

Abstract

The application discloses a kind of drug bacteriostatic detection device and detection method, more particularly to biological medicine detection technical field, including detection box and hinged in the visual sealing door of detection box front side, further include: rotary tray, rotation is installed in the bottom of the detection box inner chamber, for carrying multiple detection well plate.The drug bacteriostatic detection device and detection method of the application, by integrating rotary tray, environmental regulation module, sample adding module, optical detection module, data processing module and central controller, constructs a kind of full automatic drug bacteriostatic detection platform.The device can automatically complete the distribution of bacterial solution and drug solution, the accurate regulation of culture environment, the real-time detection of multiple well plate turbidity or fluorescence intensity and the automatic analysis and processing of data, simplifies operation process, reduces the error caused by manual intervention, improves detection efficiency and the objectivity of result, especially suitable for the bacteriostatic detection scene of multiple samples, high throughput.

Description

Technical Field

[0001] This invention relates to the field of biomedical detection technology, and in particular to a drug antibacterial detection device and detection method. Background Technology

[0002] Drug antimicrobial susceptibility testing, also known as antimicrobial drug sensitivity testing, is a crucial method for assessing the inhibitory effect of drugs on microorganisms. The results are of significant reference value for new drug development, rational drug use in clinical practice, and food safety monitoring. Currently, commonly used drug antimicrobial testing methods in the industry mainly include agar diffusion methods such as the paper disc diffusion method, dilution methods such as the broth dilution method, and the E-test method. However, traditional testing techniques have certain limitations in practical operation:

[0003] First, while the conventional agar diffusion method is simple to operate, the detection cycle is relatively long, typically requiring 24 to 48 hours to observe results. Furthermore, measuring the diameter of the inhibition zone largely depends on manual operation, leading to strong subjectivity and poor repeatability. Although some automated analytical devices incorporate image acquisition and processing technology, they are often structurally complex and costly, and their control over the temperature and pH of the culture environment is not precise enough, making the test results susceptible to fluctuations in the external environment, thus requiring improved stability.

[0004] Secondly, existing technologies often face challenges in handling trace or multiple sample detections, including cumbersome operation, high reagent consumption, and difficulty in achieving real-time dynamic monitoring. For example, while traditional microdilution methods can quantitatively determine the minimum inhibitory concentration (MIC), the manual dispensing and gradient dilution process is not only time-consuming but also prone to introducing operational errors. For some microorganisms with specific growth conditions, such as anaerobic bacteria or strains requiring specific pH environments, conventional detection devices struggle to provide a stable and suitable culture environment, limiting their application scope.

[0005] Finally, with the development of biotechnology and optoelectronic detection technology, new detection methods based on mass spectrometry, microfluidic chips, or nanoprobes have emerged in recent years. Although these methods have improved detection sensitivity or speed in some aspects, most of them are highly dependent on equipment, have complex sample pretreatment, or have high detection costs, making them difficult to popularize and promote in ordinary laboratories or primary healthcare institutions. Summary of the Invention

[0006] The main objective of this invention is to provide a drug antibacterial detection device and detection method, which can effectively solve the problems of strong subjectivity, poor repeatability, inaccurate control of the culture environment, and difficulty in achieving high-throughput real-time dynamic monitoring of multiple samples caused by the reliance on manual operation in the detection process in the prior art.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] A drug antibacterial detection device includes a detection chamber and a visually sealed door hinged to the front of the detection chamber, and further includes:

[0009] A rotating tray is rotatably mounted at the bottom of the inner cavity of the detection box to support multiple detection plates.

[0010] An environmental control module, located inside the testing chamber, is used to monitor and adjust the temperature, humidity, and pH value inside the testing chamber in real time.

[0011] The sample dispensing module, installed inside the detection chamber, is used to automatically dispense bacterial solution, drug solution, and culture medium into the detection well plate.

[0012] An optical detection module, located inside the detection chamber, is used to detect the turbidity or fluorescence intensity of the bacterial solution in each detection well plate in real time.

[0013] The data processing module is electrically connected to the optical detection module and is used to receive and analyze the detection data.

[0014] The central controller is electrically connected to the environmental control module, the sample addition module, the optical detection module, and the data processing module, respectively.

[0015] Preferably, the top of the rotating tray is provided with a plurality of positioning rods arranged in a circular array, and the detection plate is detachably mounted on the positioning rods.

[0016] Preferably, the environmental control module includes: a pH sensor, a humidity sensor, a temperature sensor, a heating element, a humidifying element, and an acid-base adjustment unit. The pH sensor, humidity sensor, and temperature sensor are all installed on the outer wall of the detection chamber, and their probes penetrate into the inner cavity of the detection chamber for real-time acquisition of environmental parameters. The heating element and humidifying element are installed on the outside of the detection chamber, and their output ends penetrate into the inner cavity of the detection chamber for adjusting temperature and humidity. The acid-base adjustment unit is installed on the outer wall of the detection chamber for adjusting pH value. The pH sensor, humidity sensor, temperature sensor, heating element, humidifying element, and acid-base adjustment unit are all electrically connected to the central controller.

[0017] Preferably, the acid-base adjustment unit includes an acid storage bottle, an alkali storage bottle, a micro-liquid pump, and a three-way valve. The two input ends of the three-way valve are respectively connected to the acid storage bottle and the alkali storage bottle, and the output end of the three-way valve is connected to the input end of the micro-liquid pump. The output end of the micro-liquid pump extends through the inner cavity of the detection chamber and is connected to an atomizing nozzle.

[0018] Preferably, the sample loading module includes a storage tank, a micro-injection pump, a multi-channel pipette tip, and a three-dimensional moving mechanism. The storage tank is installed inside the detection box. The input end of the micro-injection pump is connected to the storage tank. The multi-channel pipette tip is connected to the output end of the micro-injection pump through an infusion tube. The three-dimensional moving mechanism is installed inside the detection box and is used to drive the multi-channel pipette tip to move in the horizontal, vertical, and forward / backward directions.

[0019] Preferably, the three-dimensional moving mechanism includes an X-axis electric slide rail, a Z-axis electric slide rail, and a Y-axis electric push rod. The Z-axis electric slide rail is slidably mounted on the X-axis electric slide rail, and the Y-axis electric push rod is slidably mounted on the Z-axis electric slide rail. The output end of the Y-axis electric push rod is connected to a pipette tip mounting block, and the multi-channel pipette tip is detachably mounted on the pipette tip mounting block.

[0020] Preferably, the optical detection module includes a light source sensor and a photoelectric sensor. The light source sensor is installed at the top of the detection box, and the photoelectric sensor is installed at the bottom or side of the detection box. Both the light source sensor and the photoelectric sensor are electrically connected to the central controller. The light source sensor is a tunable multi-wavelength light source, and the photoelectric sensor is a CCD image sensor or a photomultiplier tube.

[0021] Preferably, the rotating tray is driven by a stepper motor, which is electrically connected to the central controller and is used to drive the rotating tray to rotate at a preset angle and time interval. The data processing module is electrically connected to the central controller and is used to generate microbial growth curves and inhibition rate curves, and automatically determine the minimum inhibitory concentration.

[0022] A method for detecting drug antibacterial activity based on the above-mentioned device includes the following steps:

[0023] Step S1: Load the microbial solution to be tested and the drug solution of different concentrations into different well positions of the detection plate, and align the detection plate on the positioning rod on the top of the rotating tray.

[0024] Step S2: Set the culture parameters through the central controller, start the environmental control module, and adjust the temperature, humidity and pH value in the detection chamber to the preset range.

[0025] Step S3: Start the rotating tray to rotate at a preset time interval, so that each detection aperture plate passes through the detection area of ​​the optical detection module in sequence, and the optical detection module collects the optical signals of each aperture in real time.

[0026] Step S4: The data processing module plots the microbial growth curves at various drug concentrations based on the collected optical signals, and calculates the antibacterial rate at different time points based on the growth curves.

[0027] Step S5: The data processing module automatically determines the minimum inhibitory concentration of the drug against the microorganism to be tested based on the trend of the antibacterial rate.

[0028] Step S6: The data processing module compares the detection results with the preset quality control standards. If the results exceed the quality control range, an alarm will be automatically triggered and a re-detection will be prompted.

[0029] Preferably, in step S3, the detection frequency of the optical detection module is automatically adjusted according to the growth rate of the microorganisms: the detection frequency is lower during the lag phase and the early stage of the logarithmic growth phase; and the detection frequency is automatically increased during the middle and late stages of the logarithmic growth phase.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. This invention provides a drug antibacterial detection device and method. By integrating a rotating tray, an environmental control module, a sample dispensing module, an optical detection module, a data processing module, and a central controller, a fully automated drug antibacterial detection platform is constructed. This device can automatically complete the distribution of bacterial and drug solutions, precise control of the culture environment, real-time detection of turbidity or fluorescence intensity in multi-well plates, and automatic data analysis and processing. It simplifies the operation process, reduces errors caused by manual intervention, and improves detection efficiency and the objectivity of results, making it particularly suitable for multi-sample, high-throughput antibacterial detection scenarios.

[0032] 2. This invention provides a drug antibacterial detection device and method. By incorporating an environmental control module including a pH sensor, humidity sensor, temperature sensor, heating element, humidification element, and acid-base adjustment unit, it achieves real-time monitoring and closed-loop feedback control of temperature, humidity, and pH within the detection chamber. In particular, the acid-base adjustment unit employs a structure consisting of an acid storage bottle, an alkali storage bottle, a three-way valve, a micro-pump, and an atomizing nozzle. This allows for precise pH adjustment within the chamber via atomization, providing a highly stable and controllable culture environment for microbial growth, effectively ensuring the accuracy and repeatability of the detection results.

[0033] 3. This invention provides a drug antibacterial detection device and method. Through the stepping rotation of a rotating tray in conjunction with an optical detection module, dynamic alternating detection of multiple detection wells is achieved. Combined with a data processing module, it automatically plots microbial growth curves, calculates the antibacterial rate, and determines the minimum inhibitory concentration, thus improving the intelligence level of the detection. Simultaneously, the detection frequency of the optical detection module can be automatically adjusted according to the microbial growth stage, increasing the sampling density during the logarithmic growth phase. This not only optimizes data acquisition efficiency but also more accurately captures the growth dynamics of microorganisms, providing more detailed data support for evaluating the antibacterial effect. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0035] Figure 2 This is a schematic diagram of the detection orifice plate structure of the present invention;

[0036] Figure 3 This is a schematic diagram of the environmental control module structure of the present invention;

[0037] Figure 4 This is a schematic diagram of the sample addition module structure of the present invention;

[0038] Figure 5 This is a schematic diagram of the three-dimensional moving mechanism structure of the present invention;

[0039] Figure 6 This is a schematic diagram of the acid-base adjustment unit and optical detection module of the present invention.

[0040] In the diagram: 1. Detection box; 2. Rotating tray; 21. Positioning rod; 22. Detection orifice plate; 3. Visual sealing door; 4. Central controller; 5. Environmental control module; 51. pH sensor; 52. Humidity sensor; 53. Temperature sensor; 54. Heating element; 55. Humidifying element; 56. Acid-base adjustment unit; 561. Acid storage bottle; 562. Alkali storage bottle; 563. Three-way tube; 564. Micro-volume pump; 565. Atomizing nozzle; 6. Sample dispensing module; 61. Storage tank; 62. Micro-injection pump; 63. Infusion tubing; 64. Multi-channel pipette tip; 65. Three-dimensional moving mechanism; 651. X-axis electric slide rail; 652. Z-axis electric slide rail; 653. Y-axis electric push rod; 654. Pipette tip mounting block; 7. Optical detection module; 71. Light source sensor; 72. Photoelectric sensor; 8. Data processing module. Detailed Implementation

[0041] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0042] Example 1

[0043] like Figures 1 to 6 As shown, a drug antibacterial detection device includes a detection chamber 1 and a visual sealing door 3 hinged to the front of the detection chamber 1. The detection chamber 1 is made of stainless steel, which has good sealing performance and corrosion resistance. The visual sealing door 3 allows operators to observe the internal experimental process without opening the chamber door.

[0044] A rotating tray 2 is rotatably mounted at the bottom of the inner cavity of the inspection box 1 to support multiple inspection plates 22. Multiple positioning rods 21 arranged in a circular array are provided on the top of the rotating tray 2, and the inspection plates 22 are detachably mounted on the positioning rods 21. The rotating tray 2 is driven by a stepper motor, which is electrically connected to a central controller 4, to drive the rotating tray 2 to rotate at preset angles and time intervals, thereby enabling the sequential inspection of the multiple inspection plates 22.

[0045] An environmental control module 5 is installed inside the testing chamber 1 to monitor and adjust the temperature, humidity, and pH value inside the testing chamber 1 in real time. The environmental control module 5 includes a pH sensor 51, a humidity sensor 52, a temperature sensor 53, a heating element 54, a humidifying element 55, and an acid-base adjustment unit 56. The pH sensor 51, humidity sensor 52, and temperature sensor 53 are all mounted on the outer wall of the testing chamber 1, with their probes penetrating into the inner cavity of the testing chamber 1, for real-time acquisition of environmental parameters. The heating element 54 and humidifying element 55 are mounted on the outside of the testing chamber 1, with their output terminals penetrating into the inner cavity of the testing chamber 1, for adjusting temperature and humidity. The acid-base adjustment unit 56 is mounted on the outer wall of the testing chamber 1 for adjusting the pH value. The pH sensor 51, humidity sensor 52, temperature sensor 53, heating element 54, humidifying element 55, and acid-base adjustment unit 56 are all electrically connected to the central controller 4.

[0046] Furthermore, such as Figure 3 As shown, the acid-base adjustment unit 56 includes an acid storage bottle 561, an alkali storage bottle 562, a micro-pump 564, and a three-way connector 563. The two input ends of the three-way connector 563 are connected to the acid storage bottle 561 and the alkali storage bottle 562, respectively, and the output end of the three-way connector 563 is connected to the input end of the micro-pump 564. The output end of the micro-pump 564 extends into the inner cavity of the detection chamber 1 and is connected to an atomizing nozzle 565, used to uniformly spray acid or alkali solution to achieve precise adjustment of the pH value within the chamber.

[0047] The detection chamber 1 is also equipped with a sample dispensing module 6, which is used to automatically dispense bacterial solution, drug solution, and culture medium into the detection well plate 22. Figure 4 and Figure 5As shown, the sample loading module 6 includes a storage tank 61, a micro-injection pump 62, a multi-channel pipette tip 64, and a three-dimensional movement mechanism 65. The storage tank 61 is installed inside the detection chamber 1. The input end of the micro-injection pump 62 is connected to the storage tank 61, and the multi-channel pipette tip 64 is connected to the output end of the micro-injection pump 62 via an infusion tube 63. The three-dimensional movement mechanism 65 is installed inside the detection chamber 1 and is used to drive the multi-channel pipette tip 64 to move horizontally, vertically, and forward / backward.

[0048] The three-dimensional movement mechanism 65 includes an X-axis electric slide rail 651, a Z-axis electric slide rail 652, and a Y-axis electric push rod 653. The Z-axis electric slide rail 652 is slidably mounted on the X-axis electric slide rail 651, and the Y-axis electric push rod 653 is slidably mounted on the Z-axis electric slide rail 652. The output end of the Y-axis electric push rod 653 is connected to a pipette tip mounting block 654, and a multi-channel pipette tip 64 is detachably mounted on the pipette tip mounting block 654. Through three-dimensional movement, the sample dispensing module 6 can accurately distribute liquid into the designated well positions of each detection plate 22.

[0049] An optical detection module 7 is also installed inside the detection chamber 1 to detect the turbidity or fluorescence intensity of the bacterial solution in each detection well plate 22 in real time. The optical detection module 7 includes a light source sensor 71 and a photoelectric sensor 72. The light source sensor 71 is installed at the top of the detection chamber 1, and the photoelectric sensor 72 is installed at the bottom or side of the detection chamber 1. The light source sensor 71 is a tunable multi-wavelength light source that can switch wavelengths according to the type of microorganism or detection requirements; the photoelectric sensor 72 is a CCD image sensor or a photomultiplier tube used to receive light signals and convert them into electrical signals. Both the light source sensor 71 and the photoelectric sensor 72 are electrically connected to the central controller 4.

[0050] The data processing module 8 is electrically connected to the optical detection module 7, and is used to receive and analyze detection data, generate microbial growth curves and inhibition rate curves, and automatically determine the minimum inhibitory concentration. The data processing module 8 is also electrically connected to the central controller 4 to achieve data interaction and control coordination.

[0051] Example 2

[0052] The difference between this embodiment and Embodiment 1 is that the light source sensor 71 of the optical detection module 7 adopts a tunable multi-wavelength light source, which can switch the detection wavelength according to different microbial species or detection needs, further improving the sensitivity and specificity of detection. The photoelectric sensor 72 adopts a photomultiplier tube, which is suitable for the detection of weak fluorescence signals and is suitable for experimental scenarios with low concentrations of microorganisms or low expression of fluorescent labels.

[0053] Example 3

[0054] In this embodiment, the driving method of the three-dimensional moving mechanism 65 can be a servo motor combined with a lead screw drive to improve positioning accuracy and motion stability. Multiple multi-channel pipette tips 64 can be integrated on the tip mounting block 654 to achieve parallel sample loading of multiple groups, improving detection efficiency.

[0055] The working principle of the antibacterial detection device and detection method for this drug will be explained in detail below.

[0056] like Figure 1-6 As shown, firstly, the microbial culture to be tested and the drug solutions of different concentrations are loaded into different wells of the detection plate 22, and the detection plate 22 is aligned and placed on the positioning rod 21 on the top of the rotating tray 2. The visual sealing door 3 is closed to ensure that the detection chamber 1 is sealed. Next, the culture parameters are set by the central controller 4, and the environmental control module 5 is activated to monitor and adjust the temperature, humidity and pH value inside the detection chamber 1 to the preset range in real time. The heating element 54 and the humidifying element 55 are adjusted according to the feedback from the temperature sensor 53 and the humidity sensor 52. The acid-base adjustment unit 56 sprays acid or alkali solution into the chamber through the micro-liquid pump 564 and the atomizing nozzle 565 according to the feedback from the pH sensor 51 to achieve precise pH control. Then, the rotating tray 2 is started, and the stepper motor drives the rotating tray 2 to rotate at preset time intervals, so that each detection plate 22 passes through the optical... In the detection area of ​​detection module 7, optical detection module 7 collects optical signals from each well in real time, including turbidity or fluorescence intensity, and transmits the signals to data processing module 8. Data processing module 8 plots microbial growth curves at various drug concentrations based on the collected optical signals, and calculates the inhibition rate at different time points based on the growth curves. Based on the trend of the inhibition rate, data processing module 8 automatically determines the minimum inhibitory concentration of the drug for the tested microorganism. The detection frequency of optical detection module 7 can be automatically adjusted according to the growth rate of the microorganism: the detection frequency is lower during the lag phase and the early stage of the logarithmic growth phase; the detection frequency is automatically increased during the middle and late stages of the logarithmic growth phase to capture rapidly changing growth dynamics. Finally, data processing module 8 compares the detection results with preset quality control standards. If the results exceed the quality control range, the system automatically alarms and prompts for retesting to ensure the accuracy and reliability of the detection results.

[0057] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.

Claims

1. A drug antibacterial detection device, comprising a detection chamber (1) and a visual sealing door (3) hinged to the front side of the detection chamber (1), characterized in that: Also includes: A rotating tray (2) is rotatably mounted at the bottom of the inner cavity of the detection box (1) to support multiple detection orifice plates (22). An environmental control module (5) is installed inside the detection box (1) to monitor and adjust the temperature, humidity and pH value inside the detection box (1) in real time. The sample dispensing module (6) is installed inside the detection box (1) and is used to automatically dispense bacterial solution, drug solution and culture medium into the detection well plate (22); An optical detection module (7) is installed inside the detection box (1) and is used to detect the turbidity or fluorescence intensity of the bacterial solution in each detection well plate (22) in real time. The data processing module (8) is electrically connected to the optical detection module (7) and is used to receive and analyze detection data; The central controller (4) is electrically connected to the environmental control module (5), the sample addition module (6), the optical detection module (7), and the data processing module (8), respectively.

2. The drug antibacterial detection device according to claim 1, characterized in that: The rotating tray (2) is provided with a plurality of positioning rods (21) arranged in a ring array on the top, and the detection plate (22) is detachably installed on the positioning rods (21).

3. The drug antibacterial detection device according to claim 1, characterized in that: The environmental control module (5) includes: a pH sensor (51), a humidity sensor (52), a temperature sensor (53), a heating element (54), a humidifying element (55), and an acid-base adjustment unit (56). The pH sensor (51), humidity sensor (52), and temperature sensor (53) are all installed on the outer wall of the detection box (1), and the probes penetrate into the inner cavity of the detection box 1 for real-time collection of environmental parameters. The heating element (54) and humidifying element (55) are installed on the outside of the detection box (1), and the output ends penetrate into the inner cavity of the detection box (1) for adjusting temperature and humidity. The acid-base adjustment unit (56) is installed on the outer wall of the detection box (1) for adjusting pH value. The pH sensor (51), humidity sensor (52), temperature sensor (53), heating element (54), humidifying element (55), and acid-base adjustment unit (56) are all electrically connected to the central controller (4).

4. The drug antibacterial detection device according to claim 3, characterized in that: The acid-base adjustment unit (56) includes an acid storage bottle (561), an alkali storage bottle (562), a micro-liquid pump (564), and a three-way pipe (563). The two input ends of the three-way pipe (563) are connected to the acid storage bottle (561) and the alkali storage bottle (562) respectively. The output end of the three-way pipe (563) is connected to the input end of the micro-liquid pump (564). The output end of the micro-liquid pump (564) extends through the inner cavity of the detection box (1) and is connected to an atomizing nozzle (565).

5. The drug antibacterial detection device according to claim 1, characterized in that: The sample loading module (6) includes a storage tank (61), a micro-injection pump (62), a multi-channel pipette tip (64), and a three-dimensional moving mechanism (65). The storage tank (61) is installed inside the detection box (1). The input end of the micro-injection pump (62) is connected to the storage tank (61). The multi-channel pipette tip (64) is connected to the output end of the micro-injection pump (62) through an infusion tube (63). The three-dimensional moving mechanism (65) is installed inside the detection box (1) and is used to drive the multi-channel pipette tip (64) to move in the horizontal, vertical, and forward and backward directions.

6. The drug antibacterial detection device according to claim 5, characterized in that: The three-dimensional moving mechanism (65) includes an X-axis electric slide rail (651), a Z-axis electric slide rail (652), and a Y-axis electric push rod (653). The Z-axis electric slide rail (652) is slidably mounted on the X-axis electric slide rail (651), and the Y-axis electric push rod (653) is slidably mounted on the Z-axis electric slide rail (652). The output end of the Y-axis electric push rod (653) is connected to a pipette tip mounting block (654), and the multi-channel pipette tip (64) is detachably mounted on the pipette tip mounting block (654).

7. The drug antibacterial detection device according to claim 1, characterized in that: The optical detection module (7) includes a light source sensor (71) and a photoelectric sensor (72). The light source sensor (71) is installed at the top inside the detection box (1), and the photoelectric sensor (72) is installed at the bottom or side inside the detection box (1). Both the light source sensor (71) and the photoelectric sensor (72) are electrically connected to the central controller (4). The light source sensor (71) is a tunable multi-wavelength light source, and the photoelectric sensor (72) is a CCD image sensor or a photomultiplier tube.

8. The drug antibacterial detection device according to claim 1, characterized in that: The rotating tray (2) is driven by a stepper motor, which is electrically connected to the central controller (4) and is used to drive the rotating tray (2) to rotate at a preset angle and time interval. The data processing module (8) is electrically connected to the central controller (4) and is used to generate microbial growth curves and antibacterial rate curves, and automatically determine the minimum antibacterial concentration.

9. A method for detecting drug antibacterial activity based on the above-mentioned device, characterized in that: Includes the following steps: Step S1: Load the microbial solution to be tested and the drug solution of different concentrations into different well positions of the detection plate (22), and align the detection plate (22) with the positioning rod (21) on the top of the rotating tray (2); Step S2: Set the culture parameters through the central controller (4), start the environmental control module (5), and adjust the temperature, humidity and pH value in the detection chamber (1) to the preset range; Step S3: Start the rotating tray (2) to rotate at a preset time interval, so that each detection hole plate (22) passes through the detection area of ​​the optical detection module (7) in sequence, and the optical detection module (7) collects the optical signal of each hole position in real time; Step S4: The data processing module (8) plots the microbial growth curves at each drug concentration based on the collected optical signals, and calculates the antibacterial rate at different time points based on the growth curves; Step S5: The data processing module (8) automatically determines the minimum inhibitory concentration of the drug against the microorganism to be tested based on the trend of the antibacterial rate change; Step S6: The data processing module (8) compares the detection results with the preset quality control standards. If the results exceed the quality control range, an alarm will be automatically triggered and a re-detection will be prompted.

10. The drug antibacterial detection device and detection method according to claim 9, characterized in that: In step S3, the detection frequency of the optical detection module (7) is automatically adjusted according to the growth rate of the microorganism: the detection frequency is lower during the sluggish growth phase and the early stage of the logarithmic growth phase; and the detection frequency is automatically increased during the middle and late stages of the logarithmic growth phase.

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