Preparation method and application of a glutamic-pyruvic transaminase biosensor

By utilizing the CuCo/C nanocage structure and the carbon nanotube network formed by high-temperature pyrolysis, the problem of high cost and insufficient sensitivity of traditional alanine aminotransferase detection equipment is solved, achieving real-time detection with high sensitivity and accuracy, suitable for bedside or home applications.

CN122150352APending Publication Date: 2026-06-05NANJING TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING TECH UNIV
Filing Date
2026-01-29
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Existing alanine aminotransferase (ALT) detection equipment is expensive, complex to operate, and lacks real-time capability. Traditional electrochemical sensors are not sensitive enough to meet the real-time dynamic monitoring needs of bedside or home use.

Method used

Using CuCo/C nanocage structures as electrode materials, a highly efficient three-dimensional conductive network was constructed by controlling the micro-nano structure design, combining polyvinylpyrrolidone shell protection and high-temperature pyrolysis to form a carbon nanotube network, thereby enhancing electron transfer capability. Furthermore, the electrocatalytic decomposition capability of Cu and Co bimetallic active sites was utilized to prepare a biosensor for alanine aminotransferase.

Benefits of technology

It achieves highly sensitive and accurate detection of alanine aminotransferase, with a wide linear range, simple preparation process, low cost, and is suitable for large-scale production, meeting the needs of real-time detection.

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Abstract

The application belongs to the technical field of biomedical detection and electrochemical sensor, and relates to a preparation method and application of a glutamate-pyruvate transaminase biosensor. A soluble metal salt containing cobalt cyanide ions is added into deionized water; copper salt and a complexing agent are uniformly dispersed with deionized water, and after mixing, a complexing reaction is carried out, and then filtration is carried out; the obtained solid is washed and dried, and then is uniformly mixed with polyvinylpyrrolidone, centrifuged, calcined, and CuCo / C nanoparticles are obtained, which are fixed on a gold electrode to obtain a working electrode. Glutamate oxidase, L-alanine and alpha-ketoglutaric acid are uniformly mixed with chitosan to obtain an enzyme solution, and a glutamate-pyruvate transaminase sensor is obtained by dropwise addition. The glutamate-pyruvate transaminase biosensor prepared by the application has a wide linear range and good sensitivity.
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