A method for identifying medicinal rhubarb and tangut rhubarb based on DNA fragment repeatability difference

By using specific primer pairs for PCR amplification and optimizing the DNA extraction process, the problems of low accuracy and efficiency in rhubarb identification methods have been solved, enabling efficient and accurate identification of medicinal rhubarb and Tangut rhubarb, suitable for rapid detection in general laboratories.

CN122168737APending Publication Date: 2026-06-09WUHAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV OF TECH
Filing Date
2026-05-06
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing methods for identifying rhubarb suffer from poor accuracy, low efficiency, and complex operation, making it difficult to meet the industrialization needs for rapid, high-throughput, and accurate identification of Chinese medicinal materials. In particular, they perform poorly in distinguishing between medicinal rhubarb and Tangut rhubarb.

Method used

PCR amplification was performed using specific primer pairs (ITS2F and ITS3R), and samples were treated with 0.1% ascorbic acid solution. The DNA extraction process was optimized, and the integrated design of silica gel and breathable centrifuge tubes simplified the operation steps and reduced equipment and reagent requirements, making it suitable for general molecular biology laboratories.

Benefits of technology

It significantly improves the identification accuracy of medicinal rhubarb and Tangut rhubarb, enhances sample pretreatment efficiency, reduces operational complexity and cost, is applicable to both fresh and processed products, and possesses good versatility and high-throughput detection capabilities.

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Abstract

This invention discloses a method for identifying medicinal rhubarb and tanguticum rhubarb based on differences in DNA fragment repeatability, belonging to the field of traditional Chinese medicine identification technology. The method includes the following steps: collecting three-year-old medicinal rhizome samples of the rhubarb to be tested; extracting total DNA; performing PCR amplification using specific primer pairs; sequencing the amplified products to obtain ITS2 and / or psbA-trnH sequences; comparing the sequenced sequences with a database; and identifying the sample as medicinal rhubarb (Rheum officinale) or tanguticum rhubarb (Rheum tanguticum) based on sequence homology. This invention significantly improves the accuracy and efficiency of identification by optimizing sample pretreatment conditions and improving the PCR amplification procedure, making it suitable for rapid and accurate identification of traditional Chinese medicinal materials.
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