Sealant for milk allergen component-specific ige antibody test strip and use thereof

Abstract

The application discloses a blocking agent for milk allergen component-specific IgE antibody test strips and application, and belongs to the technical field of immune detection and analysis. The blocking agent uses soybean protein isolate and fish gelatin as the blocking agent in antigen-antibody reaction of immunoserological technology, so as to reduce false negative results caused by using conventional blocking agents, casein and bovine serum albumin, and thus makes the detection result more accurate.

Claims

1. A blocking agent for a milk allergen component-specific IgE antibody test strip, characterized in that, The sealing agent includes soy protein isolate and fish gelatin.

2. The sealing agent as described in claim 1, characterized in that, The concentration of soy protein isolate is 2.0~10g / L, and the concentration of fish gelatin is 0.5~5g / L.

3. The sealing agent as described in claim 1, characterized in that, The blocking agent does not contain casein and / or bovine serum albumin.

4. The sealing agent as described in claim 1, characterized in that, The blocking agent also includes stabilizers, surfactants, and buffer solutions.

5. The sealing agent as described in claim 4, characterized in that, The concentration of the stabilizer is 10-20 g / L, the volume ratio concentration of the surfactant is 0.05%-2%, and the pH of the buffer solution is 6.0-8.

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6. The sealing agent as described in claim 4, characterized in that, The stabilizer is selected from at least one of glucose, fructose, galactose, ribose, mannose, xylose, arabinose, sucrose, maltose, lactose, and trehalose.

7. The sealing agent as described in claim 4, characterized in that, The surfactant is selected from at least one of Tween-20, Tween-21, Tween-40, Tween-60, Tween-61, Tween-80, Tween-81, Tween-85 and Triton X-100.

8. The sealing agent as described in claim 4, characterized in that, The buffer solution is selected from at least one of PBS buffer and Tris-HCl buffer.

9. The sealing agent as described in claim 1, characterized in that, The milk allergen components include at least one of Bos d 4, Bos d 5, Bos d 6, Bos d 8, Bos d 9, Bos d 10, Bos d 11 and Bos d 12.

10. The use of the blocking agent according to any one of claims 1 to 9 in the preparation of milk allergen component-specific IgE antibody test strips.

11. A test strip for detecting milk allergen-specific IgE antibodies, the test strip comprising a detection pad and a sample application pad or labeling pad overlapping the detection pad, characterized in that, The sample pad or marking pad contains the sealing agent as described in any one of claims 1 to 9.

12. The test strip as described in claim 11, characterized in that, The test strip comprises a sample pad, a marking pad, a detection pad, and an absorption pad that are sequentially overlapped.

13. A test card for detecting specific IgE antibodies against milk allergen components, characterized in that, A test strip as described in any one of claims 11 to 12 is disposed between the card cover and the card holder.

14. A kit for detecting specific IgE antibodies against milk allergen components, characterized in that, The kit comprises the test strips as described in any one of claims 11 to 12.

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