Compound weining tablet detection with uv-visible spectrophotometer and detection method

Abstract

The application relates to the field of photometer detection, in particular to a UV-visible spectrophotometer for detecting compound Weining tablets and a detection method. After the standard solution and the chromogenic agent are mixed, the mixture needs to be mixed in a targeted manner to ensure uniform reaction, and different solution amounts and different concentrations of samples need to correspond to different shaking degrees. Low-solution-amount and low-concentration samples need high-frequency small-amplitude shaking, and high-solution-amount and high-concentration samples need low-frequency large-amplitude shaking. The UV-visible spectrophotometer for detecting compound Weining tablets and the detection method can automatically obtain different frequencies and amplitudes of shaking of the cuvettes at different concentration positions, high-frequency small-amplitude shaking is obtained at a low-concentration area, which is beneficial to sufficient mixing of trace samples, low-frequency large-amplitude shaking is obtained at a high-concentration area, which is beneficial to rapid and uniform mixing of high-viscosity samples, the color development reaction of each concentration gradient is more sufficient and uniform, and therefore the linear correlation coefficient of the standard curve and the accuracy of the determination results of the test sample are remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of photometric detection, specifically to a UV-Vis spectrophotometer and detection method for detecting Compound Weining Tablets. Background Technology

[0002] Ultraviolet-visible spectrophotometry, as one of the core technologies for quantitative analysis of drug components, is widely used in the detection of effective components in traditional Chinese medicines such as Compound Weining Tablets due to its advantages of simple operation, high sensitivity, and controllable cost. Especially in the field of polysaccharide content determination, the combination of 3,5-dinitrosalicylic acid colorimetric method and spectrophotometric detection has become a standard analytical procedure in the industry (General Rule 0401). In this detection process, a standard curve needs to be plotted using a series of reference solutions of different concentrations, and then the polysaccharide content is calculated based on the absorbance value of the sample. This process involves the continuous use of multiple cuvettes.

[0003] The following problems exist in the existing technology that have not been well resolved: most existing cuvette holders adopt a single slot design and lack a buffer positioning structure to adapt to the size fluctuations of different batches of cuvettes. After the reference solution and the colorimetric reagent are mixed, they need to be mixed specifically to ensure uniform reaction. In addition, different solution volumes and different concentrations of samples require different shaking degrees. Low solution volumes and low concentrations of samples require high-frequency small-amplitude shaking, while high solution volumes and high concentrations of samples require low-frequency large-amplitude shaking.

[0004] Existing technologies use a uniform frequency and amplitude oscillation method, which cannot achieve differentiated mixing. This can easily lead to excessive oscillation and bubble generation in low-volume, low-concentration samples, or insufficient mixing in high-volume, high-concentration samples, both of which affect the accuracy of absorbance detection. Summary of the Invention

[0005] The purpose of this invention is to provide a UV-Vis spectrophotometer and detection method for detecting Compound Weining Tablets, thereby solving the problems mentioned in the background art. To achieve the above objective, this invention provides the following technical solution: a UV-Vis spectrophotometer for detecting Compound Weining Tablets, comprising a spectrophotometer body, wherein a detection chamber is formed inside the spectrophotometer body, a movable cover is provided at the opening of the spectrophotometer body, a moving assembly is provided in the detection chamber of the spectrophotometer body, the moving assembly includes a translational slide mounted in the detection chamber, the translational slide being movable along the horizontal axis of the spectrophotometer body at the opening of the spectrophotometer body, a linear drive element is provided on the inner wall of the detection chamber at one end away from the opening, and a linear drive element is provided on the inner wall of the detection chamber at the opposite end of the translational slide. Both sides are provided with guide side plates, and the guide side plates are provided with L-shaped limiting guide grooves. The horizontal section of the limiting guide groove extends along the horizontal axis of the photometer body toward the opening. The longitudinal section of the limiting guide groove is located at the opening of the photometer body and extends vertically upward toward the opening. The translation slide is provided with lifting guide grooves corresponding to the limiting guide grooves. A guide slide rod is inserted between the limiting guide grooves and the lifting guide grooves. The guide slide rod is provided with guide rollers that roll with the limiting guide grooves and the lifting guide grooves. A cuvette holder for carrying the test sample is fixedly connected to the guide slide rod.

[0006] Preferably, the cuvette holder has multiple cuvettes for placing the samples to be tested arranged at equal intervals. The cuvette holder has annular slots corresponding to each cuvette. Annular pads are embedded in the annular slots. Elastic cards are provided on the cuvette holder along the axial direction of the annular slots corresponding to the annular slots.

[0007] Preferably, the cuvette and the annular pad are detachably connected, and a flexible pad is provided in the middle area of ​​the annular pad corresponding to the bottom of the cuvette.

[0008] Preferably, the bottom of the cuvette holder is provided with an oscillation mixing mechanism on the opposite side of the cuvette. The oscillation mixing mechanism includes a support bracket fixedly connected to the bottom of the cuvette holder. The central area of ​​the support bracket is hollow and has a through groove. The through groove is arranged along the axial direction of a plurality of annular slots. The outer wall of the support bracket is horizontally provided with a sliding track along its length. The central area of ​​the support bracket has continuous protrusions below each annular slot.

[0009] Preferably, the bottom of the detection chamber is fixedly connected to a support seat on the axis of the through groove. The support seat is provided with a longitudinally telescopic adjustment slide. An oscillating base is fixedly connected to the adjustment slide. The oscillating base is horizontally slidably connected to the support bracket. An oscillating block is hinged to the oscillating base in the direction of the through groove. A limiting roller is provided on the oscillating block. The limiting roller is located in the sliding track and can slide with the sliding track. A top block is also fixedly connected to the oscillating block, and the top block is in contact with the flexible gasket at the bottom of the annular pad.

[0010] Preferably, the oscillating block has a notch and a slot, and the oscillating substrate has an elastic limiting piece. When the elastic limiting piece abuts against the notch of the oscillating block, the oscillating block can deflect slightly on the oscillating substrate.

[0011] Preferably, the UV-Vis spectrophotometer and detection method for the compound gastric tablets include the following steps:

[0012] S1: The test solution of Compound Weining Tablets is loaded into multiple cuvettes respectively. The cuvettes are placed into the annular slots of the cuvette holder. The cuvettes are fixed by the clamping action of the annular pad and the elastic card. The flexible pad at the bottom of the annular pad buffers and protects the cuvettes.

[0013] S2: Reverse start linear drive element, cuvette holder falls back along limit guide groove and moves towards detection area, the support bracket of oscillation mixing mechanism moves synchronously with it, the protrusion in sliding track drives limit roller to drive oscillation block to deflect slightly, the top block mixes solutions of different concentrations at high frequency with small amplitude or at low frequency with large amplitude through flexible pad;

[0014] S3: After the cuvette holder reaches the detection area, the slot of the oscillation block engages and locks with the elastic limiting piece, keeping the cuvette in a stable state and starting the ultraviolet-visible spectrophotometric detection.

[0015] S4: After the test is completed, the top block of the oscillating block touches the elastic card to release the locking of the annular pad, and the operator takes out the cuvette to complete the test.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0017] In this invention, the bottom of the cuvette holder is equipped with an oscillating mixing mechanism. By setting continuous protrusions on the support according to the differences in solution concentration, the protrusions in the low concentration area are lower and denser, while the protrusions in the high concentration area are higher and sparser. As the cuvette holder retracts to the detection area, the cuvettes at different concentration positions can automatically obtain vibrations of different frequencies and amplitudes. The low concentration area obtains high-frequency, small-amplitude vibrations, which is conducive to the thorough mixing of trace samples. The high concentration area obtains low-frequency, large-amplitude vibrations, which is conducive to the rapid homogenization of high-viscosity samples. This makes the colorimetric reaction of each concentration gradient more thorough and uniform, thereby significantly improving the linear correlation coefficient of the standard curve and the accuracy of the test sample measurement results.

[0018] In this invention, during the retraction of the cuvette holder, the elastic limiting plate abuts against the notch, and the oscillating block is in a mixing mode, which can transmit vibration normally. When the cuvette holder reaches the detection area, the limiting roller moves along the concave wedge edge, causing the elastic limiting plate to switch into the slot. The oscillating block is firmly limited and the top block is in a tilted state. When the cuvette holder moves outward again after the detection is completed, the tilted top block can automatically contact the elastic card, releasing the locking of the annular pad, making it convenient for the operator to remove the cuvette.

[0019] In this invention, when the cuvette is inserted into the annular slot through the annular pad, the elastic card can firmly clamp the annular pad, keeping the cuvette stable during the test. At the same time, the flexible pad at the bottom of the annular pad can flexibly contact the top block, which not only ensures the effective transmission of shaking force, but also avoids damage to the cuvette caused by rigid impact. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the three-dimensional structure of the photometer body in this invention;

[0021] Figure 2 This is a schematic diagram of the unfolded movable cover plate on the photometer body in this invention;

[0022] Figure 3 This is a top view of the photometer body in this invention;

[0023] Figure 4 This is a three-dimensional structural diagram of the moving component and cuvette holder inside the detection chamber in this invention;

[0024] Figure 5 This is a partial three-dimensional structural unfolded view of the mobile component in this invention;

[0025] Figure 6 This is a three-dimensional structural diagram of the moving component and cuvette holder in this invention;

[0026] Figure 7 This is a top view of the three-dimensional structure of the movable component and cuvette holder in this invention;

[0027] Figure 8 This is a schematic diagram showing the cuvette holder and cuvettes and other components in this invention.

[0028] Figure 9 This is a schematic diagram showing the disassembled cuvette and annular pad in this invention;

[0029] Figure 10 This is a top view of the cuvette holder and the oscillating mixing mechanism in this invention;

[0030] Figure 11 This is a side view of the cuvette holder and the oscillating mixing mechanism in this invention;

[0031] Figure 12 This is a diagram showing the state of the oscillation lever in the retracted state of the cuvette holder in this invention.

[0032] Figure 13 This is a diagram showing the state of the oscillation lever when the cuvette holder is removed in this invention.

[0033] Figure 14 This is a schematic diagram of the oscillating block and elastic limiting plate in this invention.

[0034] In the diagram: 1. Photometer body; 11. Detection chamber; 12. Movable cover; 2. Moving assembly; 21. Translation slide; 211. Lifting guide groove; 22. Linear drive element; 23. Guide side plate; 231. Limiting guide groove; 24. Guide slide rod; 25. Guide roller; 3. Cuvette holder; 31. Cuvette; 32. Annular bayonet; 33. Annular pad; 34. Elastic card; 35. Flexible pad; 4. Oscillating mixing mechanism; 41. Bearing bracket; 42. Through groove; 43. Sliding track; 431. Protrusion; 432. Protruding wedge edge; 433. Recessed wedge edge; 5. Bearing seat; 51. Adjusting slide; 52. Oscillating base plate; 53. Oscillating lever; 54. Limiting roller; 55. Top block; 56. Notch; 57. Slot; 58. Elastic limiting piece. Detailed Implementation

[0035] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] Example

[0037] Please see Figures 1 to 14The present invention provides a technical solution: a UV-Vis spectrophotometer for detecting compound gastric tablets, including a spectrophotometer body 1, a detection chamber 11 is opened inside the spectrophotometer body 1, and a movable cover 12 is provided at the opening of the spectrophotometer body 1. The operator opens the movable cover 12 to expose the opening of the detection chamber 11, thereby placing the sample for detection. A moving component 2 is provided in the detection chamber 11 of the spectrophotometer body 1.

[0038] Specifically, the moving component 2 includes a translation slide 21 installed in the detection chamber 11. The translation slide 21 can move along the horizontal axis of the photometer body 1 at the opening of the photometer body 1. A linear drive element 22 is provided on the inner wall of the detection chamber 11 at the end away from the opening. The linear drive element 22 is preferably an electric cylinder or an electric push rod. The telescopic end of the linear drive element 22 is fixedly connected to the outer wall of the translation slide 21 to drive the translation slide 21 to slide horizontally in the detection chamber 11.

[0039] Furthermore, on the inner wall of the detection chamber 11, guide side plates 23 are provided on both sides of the translation slide 21. The guide side plates 23 are provided with L-shaped limiting guide grooves 231. The horizontal section of the limiting guide groove 231 extends towards the opening along the horizontal axis of the photometer body 1. The longitudinal section of the limiting guide groove 231 is located at the opening of the photometer body 1 and extends vertically upward towards the opening. The translation slide 21 is provided with lifting guide grooves 211 corresponding to the limiting guide grooves 231. A guide slide rod 24 is inserted between the limiting guide grooves 231 and the lifting guide grooves 211. The guide slide rod 24 is provided with guide rollers 25 that roll with the limiting guide grooves 231 and the lifting guide grooves 211. A cuvette holder 3 for carrying the test sample is fixedly connected to the guide slide rod 24.

[0040] When different solutions to be tested need to be placed, the operator manually opens the movable cover 12 to expose the opening of the photometer body 1. Then, the linear drive element 22 is activated, and its telescopic end pushes the translation slide 21 to move horizontally from the detection area of ​​the detection chamber 11 toward the opening. During this process, the guide rod 24 is restricted by the limiting guide groove 231 on the guide side plate 23 and the lifting guide groove 211 on the translation slide 21. The guide rod 24 moves horizontally synchronously with the translation slide 21 with the cooperation of the guide roller 25. When it moves to directly below the opening, the guide rod 24 enters the longitudinal section of the limiting guide groove 231. The translation slide 21 continues to move, and the guide rod 24 moves longitudinally toward the opening along the longitudinal section of the limiting guide groove 231 and the lifting guide groove 211. This lifts the cuvette holder 3 fixed on the guide rod 24 and moves it outside the opening of the photometer body 1, making it convenient for the operator to place the solution to be tested into the corresponding cuvette 31.

[0041] In this embodiment, a plurality of cuvettes 31 for placing the sample to be tested are evenly distributed inside the cuvette holder 3. The cuvette holder 3 has annular slots 32 corresponding to each cuvette 31. Annular pads 33 are embedded in the annular slots 32. Elastic cards 34 are provided in the cuvette holder 3 along the axial direction of the annular slots 32 corresponding to the annular slots 32. The outer circumference of the annular pads 33 and the inner wall of the annular slots 32 are locked in a locked state by the elastic cards 34 when fully embedded, so that the cuvettes 31 remain stable during the test.

[0042] The cuvette 31 and the annular pad 33 are detachably connected. A flexible pad 35 is provided in the middle area of ​​the annular pad 33 corresponding to the bottom of the cuvette 31. The flexible pad 35 is preferably made of silicone or rubber and has elastic deformation capability.

[0043] When the operator places the cuvette 31 containing the solution into the corresponding annular slot 32, its annular pad 33 can align with the corresponding annular slot 32. After the annular pad 33 is fully aligned with the annular slot 32, the annular pad 33 can be limited by the elastic card 34, thereby enabling the cuvette 31 to be detected stably during the detection process and avoiding shaking during the detection process.

[0044] In this embodiment, the bottom of the cuvette holder 3 is provided with an oscillation mixing mechanism 4 on the opposite side of the cuvette 31, which is used to mix the solution during the process of transporting it to the detection area.

[0045] Specifically, the oscillation mixing mechanism 4 includes a support bracket 41 fixedly connected to the bottom of the cuvette holder 3. The central area of ​​the support bracket 41 is hollow and has a through groove 42. The through groove 42 is arranged along the axial direction of several annular slots 32. The outer wall of the support bracket 41 is horizontally provided with a sliding track 43 along its length. The central area of ​​the support bracket 41 is provided with continuous protrusions 431 below each annular slot 32. The distribution of the protrusions 431 is differentiated according to the liquid concentration in the cuvette 31. In the low concentration area, the protrusions 431 are lower and more densely distributed to generate high-frequency small-amplitude shaking. In the high concentration area, the protrusions 431 are higher and more sparsely distributed to generate low-frequency large-amplitude vibration.

[0046] The bottom of the detection chamber 11 is fixedly connected to a support seat 5 on the axis of the through groove 42. The support seat 5 is provided with a longitudinally telescopic adjustment slide 51. The adjustment slide 51 is fixedly connected to an oscillating base plate 52. The oscillating base plate 52 is horizontally slidably connected to the support bracket 41. The oscillating base plate 52 is hinged to an oscillating block 53 in the direction of the through groove 42. The oscillating block 53 is provided with a limiting roller 54. The limiting roller 54 is located in the sliding rail 43 and can slide with the sliding rail 43. The oscillating block 53 is also fixedly connected to a top block 55, and the top block 55 is in contact with the flexible pad 35 at the bottom of the annular pad 33.

[0047] The oscillating block 53 has a notch 56 and a slot 57. The oscillating substrate 52 is provided with an elastic limiting piece 58. When the elastic limiting piece 58 abuts against the notch 56 of the oscillating block 53, the oscillating block 53 can deflect slightly on the oscillating substrate 52. When the elastic limiting piece 58 abuts against the slot 57 of the oscillating block 53, the oscillating block 53 is limited and fixed. The two ends of the sliding track 43 are respectively provided with a protruding wedge edge 432 and a recessed wedge edge 433.

[0048] When the linear drive element 22 reverses its action, pulling the translation slide 21 and cuvette holder 3 inward to move them into the detection area, the guide slide 24 slides downward under the guidance of the limiting guide groove 231 and the lifting guide groove 211. The cuvette holder 3 moves downward synchronously. At this time, the adjusting slide 51 limits and fixes the oscillating substrate 52 to its initial position. The oscillating substrate 52 and the support bracket 41 then slide relative to each other. In this state, the elastic limiting piece 58 abuts against the notch 56 of the oscillating block 53, and the oscillating block 53 can deflect slightly. The support bracket 41 moves along with the cuvette holder. 3. The movement drives the sliding track 43 to move. The continuous protrusions 431 on the sliding track 43 contact the limiting rollers 54 on the oscillating block 53. Under the restriction of the protrusions 431, the limiting rollers 54 are intermittently lifted, causing the top block 55 to contact the flexible pad 35. Thus, the solution in the cuvette 31 is shaken through the flexible pad 35. Since the distribution density and height of the protrusions 431 correspond to the solution concentration, the low concentration area produces high-frequency small-amplitude shaking, and the high concentration area produces low-frequency large-amplitude vibration, thereby achieving a differentiated mixing effect for solutions of different concentrations.

[0049] When the cuvette holder 3 retracts to the detection area, the limiting roller 54 on the oscillating block 53 moves along the concave wedge edge 433 at the end of the sliding track 43, and the oscillating block 53 deflects on the oscillating substrate 52, causing the elastic limiting piece 58 to disengage from the notch 56 and enter the slot 57, thus firmly limiting the oscillating block 53. At this time, the top block 55 is in a tilted state.

[0050] When the solution is removed after inspection, the linear drive element 22 pushes the cuvette holder 3 outward again. Since the top block 55 is at the highest point, the top of the top block 55 will contact the elastic card 34 at the annular slot 32, thereby releasing the annular pad 33 embedded in the annular slot 32 from the locked state, making it convenient for the operator to remove the cuvette 31 after inspection.

[0051] When the support bracket 41 moves to the opening, the oscillating block 53 moves along the raised wedge edge 432, so that the elastic limiting piece 58 re-enters the groove 56 in the oscillating block 53 and returns to the initial state, which facilitates the shaking treatment when the solution is tested later.

[0052] When using the above photometer to determine the polysaccharide content of Compound Weining Tablets, follow these steps:

[0053] Accurately measure 0 ml, 0.1 ml, 0.2 ml, 0.3 ml, 0.6 ml, and 1.2 ml of the reference solution and place them into 25 ml volumetric flasks respectively, then add water to each to 2.0 ml.

[0054] Add 1.5 ml of 3,5-dinitrosalicylic acid test solution precisely to each sample and shake well.

[0055] Place in a boiling water bath for 5 minutes, remove and immediately cool to room temperature;

[0056] Add water to the mark and shake well;

[0057] Measure the absorbance at a wavelength of 520 nm using the ultraviolet-visible spectrophotometry method (General Rule 0401), with tube 0 as a blank.

[0058] A standard curve was plotted using the concentrations of a series of reference solutions and their corresponding absorbances, and the linear regression equation was calculated.

[0059] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A UV-Vis spectrophotometer for detecting Compound Weining Tablets, characterized in that, include: A photometer body (1) is provided with a detection chamber (11) inside the photometer body (1) and a movable cover plate (12) is provided at the opening of the photometer body (1). The moving component (2) is located in the detection chamber (11) and is used to drive the carrying component to enter and exit the detection chamber (11). A cuvette holder (3) is connected to the moving component (2). The cuvette holder (3) contains a plurality of cuvettes (31). The cuvette holder (3) has annular slots (32) that correspond one-to-one with the cuvettes (31). The oscillation mixing mechanism (4) is located at the bottom of the cuvette holder (3) and is used to mix the solution before detection.

2. The UV-Vis spectrophotometer for detecting Compound Weining Tablets according to claim 1, characterized in that: The moving component (2) includes a translation slide (21), a linear drive element (22), a guide side plate (23), and a guide slide rod (24). The linear drive element (22) is fixed to the inner wall of the detection chamber (11) away from the opening, and its telescopic end is fixedly connected to the translation slide (21); The guide side plate (23) is symmetrically arranged on the inner wall of the detection chamber (11) on both sides of the translation slide (21). The guide side plate (23) has an L-shaped limiting guide groove (231). The translation slide (21) has a lifting guide groove (211). The guide slide rod (24) is inserted between the limiting guide groove (231) and the lifting guide groove (211). The cuvette holder (3) is fixed on the guide slide rod (24).

3. The ultraviolet-visible spectrophotometer for detecting Compound Weining Tablets according to claim 2, characterized in that: The moving component (2) also includes a guide roller (25), which is disposed on the guide slide (24) and rolls in cooperation with the limiting guide groove (231) and the lifting guide groove (211); The limiting guide groove (231) includes a horizontal section and a vertical section. The horizontal section extends along the horizontal axis of the photometer body (1), and the vertical section extends vertically upward toward the opening.

4. The ultraviolet-visible spectrophotometer for detecting Compound Weining Tablets according to claim 1, characterized in that: An annular pad (33) and an elastic card (34) are also provided at the annular slot (32). The annular pad (33) is embedded in the annular slot (32), and the elastic card (34) is located on the cuvette holder (3) corresponding to the annular slot (32). The annular pad (33) and the annular slot (32) are clamped together by the elastic card (34). The cuvette (31) is detachably connected to the annular pad (33). The annular pad (33) has a flexible pad (35) in the middle. The flexible pad (35) is made of silicone or rubber and is provided at the bottom of the cuvette (31).

5. The ultraviolet-visible spectrophotometer for detecting Compound Weining Tablets according to claim 1, characterized in that: The oscillation mixing mechanism (4) includes a support bracket (41), an oscillation substrate (52), and an oscillation lever (53). The support bracket (41) is fixed to the bottom of the cuvette bracket (3), and it has a through groove (42) and a sliding track (43). The sliding track (43) has a continuous protrusion (431). The bottom of the detection chamber (11) is fixed with a support seat (5), the support seat (5) is provided with an adjustment slide (51), and the oscillation base plate (52) is fixed on the adjustment slide (51) and slidably connected to the support bracket (41).

6. The ultraviolet-visible spectrophotometer for detecting Compound Weining Tablets according to claim 5, characterized in that: The oscillating block (53) is hinged to the oscillating substrate (52) and is provided with a limiting roller (54) and a top block (55). The limiting roller (54) cooperates with the sliding track (43), the top block (55) contacts the annular pad (33), and contacts the elastic card (34) to release the locking when the cuvette holder (3) moves out of the opening.

7. The ultraviolet-visible spectrophotometer for detecting Compound Weining Tablets according to claim 6, characterized in that: The protrusions (431) are distributed according to the different liquid concentrations in the cuvette (31); The protrusions (431) in the low concentration area are lower and more densely distributed, while the protrusions (431) in the high concentration area are higher and more sparsely distributed.

8. The ultraviolet-visible spectrophotometer for detecting Compound Weining Tablets according to claim 7, characterized in that: The oscillating block (53) has a notch (56) and a slot (57), and the oscillating substrate (52) has an elastic limiting piece (58); The sliding track (43) has a raised wedge edge (432) and a recessed wedge edge (433) at both ends. The raised wedge edge (432) and the recessed wedge edge (433) cooperate with the limiting roller (54) to drive the elastic limiting piece (58) to selectively abut against the notch (56) or the slot (57).

9. A detection method using a UV-Vis spectrophotometer for detecting Compound Weining Tablets, comprising using the UV-Vis spectrophotometer for detecting Compound Weining Tablets as described in any one of claims 1-8, characterized in that, Includes the following steps: S1: The compound gastric tablet solution to be tested is loaded into multiple cuvettes (31). The cuvettes (31) are placed into the annular slots (32) of the cuvette holder (3). The cuvettes (31) are fixed by the clamping of the annular pad (33) and the elastic card (34). The flexible pad (35) at the bottom of the annular pad (33) buffers and protects the cuvettes (31). S2: Reverse start linear drive element (22), cuvette holder (3) falls back along limit guide groove (231) and moves towards detection area, the support bracket (41) of oscillation mixing mechanism (4) moves synchronously with it, the protrusion (431) in sliding track (43) drives limit roller (54) to drive oscillation block (53) to deflect slightly, top block (55) mixes solutions of different concentrations at high frequency with small amplitude or at low frequency with large amplitude through flexible pad (35); S3: After the cuvette holder (3) reaches the detection area, the slot (57) of the oscillating block (53) engages and locks with the elastic limiting piece (58), the cuvette (31) remains stable, and the ultraviolet-visible spectrophotometry is started; S4: After the test is completed, the top block (55) of the oscillating block (53) touches the elastic card (34) to release the locking of the annular pad (33), and the operator takes out the cuvette (31) to complete the test.

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