Extraction process of active components of pinellia ternata

Abstract

This application discloses an extraction process for active components of Pinellia ternata, comprising the following steps: (1) preparing a Pinellia ternata suspension with a material-to-liquid ratio of 1:(15~20) g / mL; (2) using a needle-plate electrode structure and a high-frequency pulse power supply to transport the Pinellia ternata suspension to the electrode area and apply high-voltage pulse treatment; (3) concentrating the treated extract under reduced pressure to obtain the active extract. The extraction process of this application solves the problems of high extraction cost, low extraction efficiency, serious loss of active components, and low industrial production efficiency of the prior art. The extraction process of this application shortens the extraction cycle and significantly improves the extraction efficiency of active components.

Description

Technical Field

[0001] This application belongs to the field of traditional Chinese medicine extraction, and specifically relates to an extraction process for active components of Pinellia ternata. Background Technology

[0002] Traditional Chinese medicine decoction techniques suffer from several shortcomings, affecting the quality stability and clinical efficacy of herbal decoctions. For example, the standardization of process parameters is severely lacking; the water-to-material ratio relies on empirical methods, resulting in actual variations of up to ±30%, leading to fluctuations in the extraction rate of active ingredients. Temperature control depends on subjective judgment of the "high heat - low heat" transition, failing to accurately identify false boiling or achieve the optimal extraction temperature for different components. Deviations in time parameters lead to differences in the content of active components. Secondly, extraction kinetics are inefficient and component degradation is severe. Static soaking relies on passive diffusion, which suffers from triple mass transfer barriers, including intracellular diffusion, interfacial mass transfer, and osmotic pressure inhibition. Continuous heating in an open system leads to the loss of volatile components and the degradation of thermally unstable components. Furthermore, precise methods for controlling toxic components are lacking. Calcium oxalate crystals and alkaloids are affected by factors such as uneven temperature fields and dynamic pH changes, resulting in large fluctuations in detoxification efficiency, and it is impossible to monitor the residual amount of toxic components or the progress of the detoxification reaction in real time.

[0003] Traditional extraction methods for Pinellia ternata result in the near-complete degradation of amino acids and unintended reactions between multiple components, such as the precipitation of tannins and alkaloids. Furthermore, existing industrial production methods are poorly adaptable, special processing methods hinder continuous production, large-capacity decoction leads to decreased mass and heat transfer efficiency, and the open system has low thermal energy utilization. Summary of the Invention

[0004] Objective of the Invention: To address the problems existing in the prior art, this application provides an extraction process for the active components of Pinellia ternata, solving the problems of low extraction efficiency, loss of active components, and low industrial production efficiency in the prior art. The extraction process of this application shortens the extraction cycle and significantly improves the extraction efficiency of active ingredients.

[0005] Technical solution: This application provides a process for extracting active components from Pinellia ternata, including the following steps: (1) Prepare a Pinellia ternata suspension with a material-to-liquid ratio of 1:(15~20)g / mL; (2) Using a needle plate electrode structure and a high-frequency pulse power supply, the Pinellia ternata suspension is transported to the electrode area and subjected to high-voltage pulse treatment; (3) The processed extract is concentrated under reduced pressure to obtain the active extract.

[0006] In some embodiments, the spacing between the needle plate electrodes is 8mm to 10mm, and the needle plate electrodes are made of titanium-plated platinum.

[0007] In some embodiments, the flow rate of the Pinellia ternata suspension is 200 mL / min to 300 mL / min.

[0008] In some embodiments, a 2.5 to 3.5 kV high-voltage pulse is applied to the needle plate electrode.

[0009] In some embodiments, the pulse processing time is 8 to 12 seconds.

[0010] In some embodiments, the pulse processing temperature is 25°C to 40°C.

[0011] In some embodiments, a 100-150 MPa instantaneous shock wave is applied during the treatment of the Pinellia ternata suspension.

[0012] In some embodiments, the pulse width of the pulse applied during the treatment of the Pinellia ternata suspension is 10-20 mm.

[0013] In some embodiments, the electrode needles in the needle plate electrode structure are made of titanium, and the outer layer of the electrode needles has a platinum layer with a thickness of 1~3 μm.

[0014] In some embodiments, the pulse frequency is 6kHz to 8kHz.

[0015] This method uses an extraction system consisting of 100g of Pinellia ternata crude powder (containing ≥0.05% guanosine and ≥0.3% succinic acid) and 1500-2000mL of deionized water (conductivity ≤5μS / cm). The final extraction rate of guanosine reaches 82-88%, and the extraction rate of succinic acid reaches 90-95%. The entire process takes less than 15 minutes and consumes only 0.8kWh per batch, achieving efficient, low-temperature, and low-energy green extraction of active ingredients from Pinellia ternata.

[0016] Beneficial effects: This application discloses a process for extracting active components of traditional Chinese medicine, including the following steps: (1) preparing a suspension of Pinellia ternata with a material-to-liquid ratio of 1:(15~20)g / mL; (2) using a needle plate electrode structure and a high-frequency pulse power supply to transport the suspension of Pinellia ternata to the electrode area and apply high-voltage pulse treatment; (3) concentrating the treated extract under reduced pressure to obtain an active extract. Compared with existing technologies, this application has the following advantages: In terms of extraction efficiency, the extraction rates of guanosine and succinic acid are increased to 82%~88% and 90%~95%, respectively; in terms of energy conservation and environmental protection, energy consumption is reduced by 73% to 0.8kWh / batch, solvent consumption is reduced by 25%, and zero wastewater discharge is achieved; in terms of product quality, the retention rate of heat-sensitive components is >95%, the destruction rate of calcium oxalate needle crystals is ≥99%, and the batch stability of the product (RSD<3%) is significantly better than that of traditional processes; in terms of process control, parameter accuracy of ±1% is achieved, and a monitoring sensitivity of 0.01mg / mL is provided; in terms of industrial application, the equipment footprint is reduced by 60%, the daily processing capacity is increased by 20 times to 1.2 tons, and the overall production cost is reduced by 42%. These advantages stem from the unique synergistic effect of plasma technology: microsecond-level shock waves (100~150MPa) break cell walls, active free radicals (50~80μmol / L·OH) promote selective dissolution, and the electro-chemical energy conversion efficiency reaches over 85%. This fundamentally solves the industry pain points of traditional processes, such as high-temperature damage, uneven extraction, and excessive energy consumption. Experimental data shows that processing 100kg of raw materials saves 220kWh of electricity (reducing CO2 emissions by 172kg), providing an innovative solution for the modern extraction of traditional Chinese medicine. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a chromatogram of the extracted herbal components in the embodiments of this application; Figure 2 This is a schematic diagram of the liquid phase discharge plasma device in the embodiments of this application; Figure 3 This is a schematic diagram of the electrode needle structure in an embodiment of this application; Figure 4 for Figure 3 A schematic diagram of the structure of part A in the diagram; Reference numerals: 10-Reaction device, 100-Equipment body, 101-Electrode wire, 102-Fixing plate, 103-Electrical control connector, 104-Current probe, 105-Voltage probe, 106-Electrode plate, 110-Electrode needle, 111-Electrode needle body, 112-First coating, 113-Flow channel, 200-First cavity, 20-Airflow device, 21-Flow meter, 300-Second cavity, 30-Detection device, 31-Processor, 40-Pulse voltage generator, 50-Oscilloscope. Detailed Implementation

[0019] It should be noted that the following detailed descriptions are exemplary and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0020] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application. In addition, in the description of this application, the term "comprising" means "including but not limited to". The terms first, second, third, etc. are used only as illustrative purposes and do not impose numerical requirements or establish an order. Various embodiments of this application may exist in the form of a range; it should be understood that the description in the form of a range is only for convenience and conciseness and should not be construed as a hard limitation on the scope of this application; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single values ​​within that range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which applies regardless of the range. Additionally, whenever a numerical range is specified in this document, it means that any referenced number (fraction or integer) within the range is included.

[0021] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art described herein. While only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this application. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail. Unless otherwise stated, “%” means percentage based on mass.

[0022] The heat-sensitive active ingredients (such as amino acids and succinic acid) in Pinellia ternata are the key material basis for its medicinal efficacy. Guanosine has neuroprotective, anticonvulsant, and immunomodulatory effects and is an important active ingredient for treating epilepsy and central nervous system diseases, but it is heat-sensitive (easily decomposed above 60°C). Succinic acid, as a representative of organic acids, has anti-inflammatory and antiemetic effects, but it is easily volatilized or oxidized at high temperatures. Pinellia ternata also contains other heat-sensitive components, such as adenosine, which are easily lost during traditional high-temperature decoction or processing, directly affecting its core efficacy in relieving cough, resolving phlegm, and suppressing nausea and vomiting. Furthermore, traditional decoction methods cannot control the extraction efficiency of active ingredients. Secondly, the extraction kinetics are inefficient and the components are severely damaged, including degradation of heat-instantaneous components, almost complete degradation of amino acids in Pinellia ternata, and unexpected reactions between multiple components (precipitation of tannins and alkaloids). Moreover, there is a lack of precise means to control toxic components. In this application, the detection method for active substances can adopt the detection methods provided in the prior art.

[0023] Example 1: To improve the extraction efficiency of active substances from Pinellia ternata, this application provides a structure of liquid-phase discharge plasma. The structure of the liquid-phase discharge plasma of this application is as follows: Figure 2 As shown, the liquid phase discharge plasma device in this embodiment includes a reaction device 10, which includes a device body 100. In some embodiments, the device body 100 has a cylindrical structure and is made of a non-conductive material, such as acrylic. In some specific embodiments, the height of the device body 100 is 20cm and the diameter is 30cm. The volume of the device body 100 can be adjusted as needed. Figure 2 and Figure 3 As shown, the device body 100 is provided with a plurality of electrode needles 110. In some embodiments, the plurality of electrode needles 110 are evenly distributed in a circular shape inside the device body 100 and are fixed by a fixing plate 102.

[0024] In some embodiments, there is a gap between the bottom ends of several electrode needles 110 and the bottom of the device body 100, for gas to be sent from the airflow device 20 to the bottom of the device body 100. The fixing plate 102 and the device body 100 form a first cavity 200, and the first cavity 200 is connected to the air outlet of the airflow device 20. The gas sent from the airflow device 20 is distributed in the first cavity 200. The gas is sent from the gap at the bottom of the electrode needles 110 into the flow channel 113 of the electrode needles 110. The gas is sent directly out from the electrode needles, which can promote plasma generation and explosion, and improve the extraction efficiency of active substances.

[0025] In some embodiments, the bottom of the electrode needle 110 is connected to a pulse voltage generator 40 via an electrode wire 101, and the pulse voltage generator 40 provides AC high-voltage pulse power. The electrode needle 110 serves as the high-voltage electrode of the reaction device 10, connected via the electrode wire 101. An electrode plate 106, a low-voltage electrode, is disposed above the electrode needle 110 and is welded to the inner wall of the device body 100. In some embodiments, the distance between the tip of the electrode needle 110 and the electrode plate 106 is 8mm to 15mm.

[0026] In some embodiments, the top of the electrode plate 106 is connected to an oscilloscope 50, and the voltage probe 105 and current probe 104 are used to detect the waveforms of the voltage and current between the two electrodes and display and store them on the oscilloscope 50.

[0027] In some embodiments, the electrode plate 106, the fixing plate 102, and the side wall of the device body 100 form a second cavity 300, which is connected to the detection device 30. In some specific embodiments, the detection device 30 is a high-performance liquid chromatograph. The processed drug solution is tracked and detected by high-performance liquid chromatography and analyzed by processor 31. The processor 31 can be a computer device dedicated to the detection device 30.

[0028] In some embodiments, the electrode needles 110 in this application are distributed in multiple circumferential layers, such as using 7 to 10 electrode needles 110. In some specific embodiments, the electrode needle 110 includes an electrode needle body 111, and the top of the electrode needle body 111 has a sloping structure. In some embodiments, a first coating 112 is provided on the outer side of the electrode needle body 111, and a flow channel 113 is provided on the inner side of the electrode needle body 111. This structure adopts a needle-plate electrode structure. Gas enters the electrode needle body 111 from the flow channel and exits from the top of the electrode needle body 111, allowing for more complete contact between the air and the liquid medicine. The gas and the pulse current generated by the electrode needle body 111 work together to generate more plasma to destroy the cell tissue of the medicinal material and promote the dissolution of the active ingredients. This tip discharge mode combined with gas infusion will form more plasma channels. The plasma channels contain highly ionized fluids in a high-pressure and high-temperature state. Once the plasma channels are formed, they will diffuse outward. The mechanical inertia of the surrounding water will resist this expansion, leading to the generation of extremely high pressure. The pressure change is determined by the ratio of the heating rate to the difference between the speed of sound and the expansion rate. The heating rate is the time derivative of the dissipated power. The energy stored in the plasma channel is gradually dissipated in the form of thermal radiation and mechanical work; this dissipation process is relatively slow compared to the plasma formation process. In this application, the ionization efficiency is improved through a sloped structure and a flow channel structure integrated with the electrode needle, further enhancing the extraction efficiency of the active ingredients.

[0029] Before the discharge wave, the high pressure generated in the plasma is conducted to the water interface, thus forming a strong compression wave (shock wave). Only under extremely high pressure will the compression wave propagate faster than the propagation speed of the diffused bubble, reaching several times the speed of sound (at a pressure of 6 kbar, the speed of sound is only 1.56 times higher than at normal pressure). The pressure variation range of the shock wave is between 5 and 20 kbar.

[0030] When a shock wave reaches a free surface, under pressureless conditions, the compression wave at the interface immediately transforms into a tension wave (or rarefaction wave) and is reflected back into the liquid medium, simultaneously propelling water into the air. As the rarefaction wave returns from the water, cavitation occurs. The shock wave, rarefaction wave, and bubble expansion maintain the fluid in a highly turbulent mixing state until all pressures reach equilibrium.

[0031] In some specific embodiments, the electrode needle body 111 is made of titanium metal, the diameter d1 of the electrode needle body 111 is 1 mm to 1.5 mm, the first coating 112 is made of platinum metal, the thickness of the first coating 112 is 1 μm to 3 μm, and the diameter d2 of the flow channel 113 is 0.2 mm to 0.4 mm. The titanium-plated platinum electrode of this application is mainly characterized by the following aspects: (1) Excellent corrosion resistance. The platinum coating (1~3μm) makes the corrosion rate of the electrode in a strong oxidizing environment (·OH concentration 50-80μmol / L) <0.001mm / year, and can withstand extreme environments with pH 1~14, adapting to various Chinese medicine extraction systems; (2) Optimized discharge characteristics: The electron work function (5.3eV) on the surface of the platinum coating is significantly lower than that of pure titanium (4.3eV), the discharge initiation voltage is reduced by 15~20%, the plasma luminescence efficiency is increased by 30%, and it has stable electrochemical performance, suppresses the occurrence of side reactions, adapts to high-frequency pulse conditions, and reduces energy consumption; (3) Unique surface effects: It provides more active sites, increases the bubble nucleation density by 5 times, and enhances the turbulence intensity.

[0032] Example 2: This application provides a method for continuous room-temperature extraction of active ingredients from Pinellia ternata using liquid-phase discharge plasma based on the method provided in Example 1. The method includes the following steps: First, dried Pinellia ternata tubers (originating from Yingshang County, Anhui Province) are pulverized through a 40-mesh sieve to obtain coarse powder. This powder is then mixed with deionized water at a material-to-liquid ratio of 1:15 g / mL to form a uniform suspension. Using a needle-plate electrode structure (8-10 mm spacing, titanium-plated platinum material) and a high-frequency pulse power supply (6-8 kHz), the mixture is pumped to the electrode area at a flow rate of 250 mL / min via a peristaltic pump. A high-voltage pulse of 2.5-3.5 kV is applied for 10 ± 1 s, during which the temperature is maintained at 25-40℃. This process generates a 100-150 MPa instantaneous shock wave and a turbulent velocity gradient of >500 s⁻¹. The extract is then coarsely filtered through a 100-mesh sieve and concentrated under reduced pressure at 60℃ to 1 / 5 of its original volume. Parameters for Samples 1-19, Comparative Example 1, and Comparative Example 2 are detailed in Table 1.

[0033] Parameter control: In this application, parameters are controlled in the following ways: the high-frequency pulse voltage is adjusted by changing the input voltage; the peristaltic pump is used to control the flow rate of the input raw material; the high-frequency pulse power supply is used to control the high-voltage pulse, adjusting the pulse frequency and pulse width; and the instantaneous shock wave is controlled by controlling the voltage magnitude, pulse frequency, and pulse width parameters.

[0034] Detection method for guanosine: Chromatographic column: Zorbax Eclipse Plus C 18 The column was 250 mm × 4.6 mm, 5 μm; the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution, with gradient elution: 0–25 min, 5%–18% acetonitrile; 25–30 min, 18% acetonitrile; 30–40 min, 18%–34% acetonitrile; 40–43 min, 34%–38% acetonitrile; 43–46 min, 38%–100% acetonitrile; the acquisition time was 46 min, the flow rate was 1.0 mL / min, the detection wavelength was 230 nm, the column temperature was 25 °C, and the injection volume was 10 μL.

[0035] Detection method for succinic acid: C18 column (4.6 mm × 250 mm, 5 μm); using acetonitrile (A) - 0.01% phosphoric acid solution (B) as the mobile phase, gradient elution (0.0~10.0 min, 0.5%~1.0% A; 10.01~15.00 min, 1.0%~1.5% A; 15.01~30.00 min, 1.5%~5.5% A; 30.01~35.00 min, 5.5%~10.0% A; 35.01~45.00 min, 10.0%~15.0% A; 45.01~55.00 min, 15.0%~20.0% A; 55.01~67.00 min, 20.0%~80.0%). A: 67.01~80.00 min, 80.0%~0.5% A): Flow rate 1.0 mL / min; Detection wavelength: 0~4.9 min, 195 nm; 4.91~55 min, 255 nm; Column temperature: 30 °C; Injection volume 15 μL.

[0036] Table 1 Extraction processes with different parameters in this application

[0037] Table 2 Extraction effects of extraction processes with different parameters in this application

[0038] Note: The data in Table 2 are expressed as mean values, and the number of parallel samples is 3.

[0039] As shown in Table 2, this method uses an extraction system consisting of 100g of Pinellia ternata crude powder (containing ≥0.05% guanosine and ≥0.3% succinic acid) and 1500-2000mL of deionized water (conductivity ≤5μS / cm). The final extraction rate of guanosine reaches 82-88%, and the extraction rate of succinic acid reaches 90-95%. The entire process takes less than 15 minutes and consumes only 0.8kWh / batch. This method achieves efficient, low-temperature, and low-consumption green extraction of active ingredients from Pinellia ternata. The electrode wear rate is only 0.12μg / Ah, and after 500 hours of continuous operation, the discharge parameter fluctuation is less than 3%.

[0040] As can be seen from the data of Sample 2 and Comparative Example 1, the titanium-plated platinum electrode of this application has a unique surface effect: nano-sized platinum crystals (50-100nm) provide more active sites, the bubble nucleation density is increased by 5 times (>1000 bubbles / cm²·s), and the turbulence intensity is enhanced by 30% (Reynolds number Re>5000), which can improve the total extraction rate of inosine, guanosine, thymidine and adenosine in Pinellia ternata, and can also improve the extraction efficiency of succinic acid.

[0041] As can be seen from the data of Sample 2 and Comparative Example 2 of this application, the method of introducing gas through the flow channel 113 in this application can improve the extraction efficiency of guanosine active substances and succinic acid by increasing plasma generation and the bursting ability of bubble formation.

[0042] Since the pulse frequency, high voltage pulse voltage, and pulse width all affect the instantaneous shock wave in this application, the pulse frequency is controlled at 6kHz~8kHz, the high voltage pulse voltage is controlled at 2.5~3.5kV, and the pulse width is controlled at 10~20㎲, which can control the instantaneous shock wave at 100~150MPa and improve the extraction efficiency of active substances.

[0043] The data from Sample 2, Samples 18 and 19 show that the air intake affects the turbulent velocity gradient, which in turn affects the extraction efficiency through the resulting turbulent velocity gradient. Within the limits defined in this application, the extraction efficiency of guanosine and succinic acid is improved compared to the extraction efficiency without air intake. However, due to the stability of the active substances, controlling the air intake efficiency at 1.5 L / min results in better extraction efficiency than other samples. This may be because an excessively high turbulent velocity gradient can easily damage the active ingredients, while an excessively low turbulent velocity gradient cannot achieve the desired extraction efficiency.

[0044] Comparative Example 3: This application extracts guanosine active components from Pinellia ternata using a water extraction method. The specific implementation method is as follows: Take 10 g of medicinal powder (passed through a No. 1 sieve), add 10 times the amount of water and soak for 30 minutes (i.e., 100 mL of water), then decoct for 50 minutes. After decoction, filter while hot to remove the residue, then add 8 times the amount of water (80 mL) and decoct the residue again for 40 minutes. Similarly, filter while hot and combine the two filtrates. The efficiency is compared with that of the sample extracted from Sample 2 of this application. Figure 1 The data shows that the fingerprint spectrum of the active substances extracted in this application is the same as that of the decoction. However, the extraction method of this application not only improves the extraction rate of guanosine active components, but also achieves better extraction results in 8-12 seconds while the decoction usually takes 4-12 hours.

[0045] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0046] The above provides a detailed description of the extraction process of active components of Pinellia ternata provided in the embodiments of this application. Specific examples have been used to illustrate the principles and implementation methods of this application. The description of the above embodiments is only for the purpose of helping to understand the method and core ideas of this application. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this application. Therefore, the content of this specification should not be construed as a limitation of this application.

Claims

1. A process for extracting active components from Pinellia ternata, characterized in that, Includes the following steps: (1) Prepare a Pinellia ternata suspension with a material-to-liquid ratio of 1:(15~20)g / mL; (2) Using a needle plate electrode structure and a high-frequency pulse power supply, the Pinellia ternata suspension is transported to the electrode area and subjected to high-voltage pulse treatment; (3) The processed extract is concentrated under reduced pressure to obtain the active extract.

2. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, The spacing between the needle plate electrodes is 8mm to 10mm, and the needle plate electrodes are made of titanium-plated platinum.

3. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, The flow rate of the Pinellia ternata suspension is 200 mL / min to 300 mL / min.

4. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, A 2.5–3.5 kV high-voltage pulse is applied to the needle plate electrode.

5. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, The pulse processing time is 8 to 12 seconds.

6. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, The pulse processing temperature is 25℃~40℃.

7. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, A 100-150 MPa instantaneous shock wave is applied during the treatment of the Pinellia ternata suspension.

8. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, The pulse width of the pulse applied during the treatment of the Pinellia ternata suspension is 10~20 mm.

9. The extraction process of Pinellia ternata active components according to claim 2, characterized in that, The electrode needles in the needle plate electrode structure are made of titanium, and the outer layer of the electrode needles has a platinum layer with a thickness of 1~3μm.

10. The extraction process of Pinellia ternata active components according to claim 1, characterized in that, The pulse frequency is 6kHz to 8kHz.

Images