A traditional Chinese medicine composition for treating scald and a preparation method and application thereof

Abstract

This invention relates to a traditional Chinese medicine composition for treating burns, its preparation method, and its application, belonging to the field of traditional Chinese medicine technology. It addresses the technical problems of existing external traditional Chinese medicine preparations for treating burns, which often contain numerous and complex ingredients, and suffer from incomplete extraction of effective components. The traditional Chinese medicine composition of this invention consists of the following components: 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, 10-20 parts of honeysuckle, 15-25 parts of aloe vera, and 10-30 parts of borneol. This invention is the first to combine dragon's blood, centella asiatica, aloe vera, and honeysuckle in combination, achieving excellent therapeutic effects. Together, they promote skin tissue growth, have anti-inflammatory and anti-infective properties, and inhibit scar hyperplasia. Using modern extraction technology and supported by scientific theory, the utilization rate of dragon's blood, centella asiatica, and aloe vera is greatly improved, promoting skin regeneration and inhibiting scar regeneration.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, specifically to a traditional Chinese medicine composition for treating burns, its preparation method, and its application. Background Technology

[0002] Burns are tissue injuries caused by heat factors such as high-temperature liquids (e.g., boiling water, hot oil), steam, high-temperature solids, or flames, and are common accidental injuries in daily life. In the treatment of burns, topical medication is superior to systemic medication; therefore, external treatment methods are the most common for burns, and topical medication plays a prominent role in burn treatment. Traditional Chinese medicine has rich experience in the external treatment of burns, with the ancient saying, "For sores and ulcers, external treatment is most suitable." Furthermore, traditional Chinese medicine treatment has the advantages of multi-target effects and fewer adverse reactions, making it one of the research hotspots in burn treatment.

[0003] However, current topical Chinese medicine preparations for treating burns are often complex formulas made from multiple herbs, making it difficult to identify specific active ingredients and clarifying their mechanisms of action. Furthermore, some of these medications are based on empirical use, with limited support from modern pharmacological research, and their targets are unclear. In terms of manufacturing processes, existing burn medications are relatively crude, failing to effectively collect the active ingredients. Components ineffective on the burn wound may hinder absorption by the tissues, affecting the drug's efficacy. For example, the burn ointment produced by Xiuzheng Pharmaceutical Group (Hunan) Pharmaceutical Co., Ltd. (approval number Z20020039) is composed of borneol, purple gromwell root, angelica root, angelica dahurica, dragon's blood, and licorice root. It can clear heat and relieve pain, detoxify and reduce swelling, and promote tissue regeneration, primarily used for small-area burns with heat toxicity syndrome, showing significant efficacy. However, this burn ointment has a large number of herbs in its formula, resulting in a complex composition. In terms of preparation, the herbs are directly extracted using sesame oil as a base, leading to incomplete extraction of the active ingredients. Summary of the Invention

[0004] This invention addresses the technical problems of existing topical Chinese medicine preparations for treating burns, which often contain numerous and complex ingredients, and suffer from incomplete extraction of effective components. It provides a Chinese medicine composition for treating burns, its preparation method, and its application. This invention is the first to combine dragon's blood, centella asiatica, aloe vera, and honeysuckle to form a novel Chinese medicine composition with excellent therapeutic effects. Together, they promote skin tissue growth, have anti-inflammatory and anti-infective effects, and inhibit scar hyperplasia. Utilizing modern extraction technology and supported by scientific theory, this invention significantly improves the utilization rate of dragon's blood, centella asiatica, and aloe vera, promoting skin regeneration and inhibiting scar regeneration.

[0005] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows: A traditional Chinese medicine composition for treating burns, comprising the following components by weight: 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, 10-20 parts of honeysuckle, 15-25 parts of aloe vera, and 10-30 parts of borneol. The preparation method of the traditional Chinese medicine composition for treating burns is as follows: The ethanol extracts of dragon's blood, centella asiatica and honeysuckle were extracted by ethanol reflux extraction. Add the aloe vera and the alcohol extract from the previous step to the matrix together; After the matrix cools down, borneol is added to obtain the traditional Chinese medicine composition for treating burns.

[0006] Preferably, the traditional Chinese medicine composition for treating burns comprises, by weight, the following components: 30-40 parts of dragon's blood, 30-35 parts of centella asiatica, 10-15 parts of honeysuckle, 15-20 parts of aloe vera, and 25-30 parts of borneol.

[0007] More preferably, the traditional Chinese medicine composition for treating burns comprises, by weight, the following components: 40 parts of dragon's blood, 30 parts of centella asiatica, 10 parts of honeysuckle, 15 parts of aloe vera, and 25 parts of borneol.

[0008] Preferably, the matrix is ​​one or more of petrolatum, sesame oil, and lanolin.

[0009] A method for preparing a traditional Chinese medicine composition for treating burns includes the following steps: Take 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, and 10-20 parts of honeysuckle. Add 70%-90% ethanol to extract for 1.0-2.0 hours, according to 6-8 times the total weight of dragon's blood, centella asiatica, and honeysuckle. Filter to remove residue, collect the decoction, concentrate the decoction at 70-90℃ until there is no alcohol taste, dry the concentrate under reduced pressure at 60-70℃, and pulverize it into fine powder. Grind 15-25 parts of aloe vera into a fine powder, and add it to the base along with the dry ointment powder obtained in the previous step, while stirring. Once the matrix has cooled to 55°C, add 10-30 parts of borneol while stirring until it is completely dissolved, thus obtaining the traditional Chinese medicine composition for treating burns.

[0010] Preferably, the preparation method of the traditional Chinese medicine composition for treating burns includes the following steps: Take 40 parts of dragon's blood, 30 parts of centella asiatica, and 10 parts of honeysuckle. Add 70% ethanol to extract for 1.0 h at 8 times the total weight of dragon's blood, centella asiatica, and honeysuckle. Filter to remove residue, collect the decoction, concentrate the decoction at 90℃ until there is no alcohol taste, dry the concentrate under reduced pressure at 70℃, and pulverize it into fine powder. Grind 15 parts of aloe vera into a fine powder and add it to the base along with the dry ointment powder obtained in the previous step, stirring as you add it. Once the matrix has cooled to 55°C, add 25 parts of borneol while stirring until it is completely dissolved to obtain the traditional Chinese medicine composition for treating burns.

[0011] The application of a traditional Chinese medicine composition of the present invention in the preparation of anti-inflammatory or analgesic drugs.

[0012] The application of a traditional Chinese medicine composition of the present invention in the preparation of a scald treatment drug.

[0013] The beneficial effects of this invention are: The herbal composition of this invention consists of dragon's blood, centella asiatica, aloe vera, honeysuckle, and borneol. In this formula, dragon's blood is the principal herb, primarily promoting blood circulation and tissue regeneration; centella asiatica, which promotes blood circulation and removes blood stasis, and assists dragon's blood in enhancing its tissue regeneration and wound-healing effects, serves as the assistant herb; honeysuckle, which clears heat and detoxifies, and has antibacterial and anti-inflammatory properties, serves as the adjuvant herb; and aloe vera, which guides the herbs to their respective meridians and harmonizes the other herbs, serves as the guiding herb, enhancing the overall efficacy of the formula. This formula focuses on "promoting blood circulation and tissue regeneration, clearing heat and detoxifying," while also considering antibacterial, anti-inflammatory, and repair-promoting effects. It aligns with the traditional Chinese medicine principle of "removing necrotic tissue and promoting new tissue growth" in burn treatment. Furthermore, combined with modern pharmacological research, it has good clinical practical value, solving problems such as incomplete efficacy and complex composition, and providing a more effective herbal composition for burns.

[0014] This invention utilizes modern extraction technology and is supported by scientific theory. Based on the principles of removing necrotic tissue and promoting tissue regeneration, invigorating blood circulation and removing blood stasis, and reducing inflammation and inhibiting bacteria, it improves the drug and develops a traditional Chinese medicine composition for treating burns through pharmacodynamic comparison. This invention greatly increases the utilization rate of dragon's blood, centella asiatica and aloe vera, promotes skin regeneration and inhibits scar regeneration.

[0015] The traditional Chinese medicine composition of the present invention not only has few ingredients, but also has significant functions in promoting skin tissue growth, anti-inflammatory and anti-infective effects, and inhibiting scar hyperplasia, especially in the acute and post-prognosis treatment of burns. Detailed Implementation

[0016] The inventive concept of this invention is as follows: In existing scald ointment formulas, dragon's blood is used as an adjuvant in extremely small amounts. However, recent studies have shown that dragon's blood has remarkable efficacy in promoting the growth and healing of scalded skin tissue. Research has shown that high, medium, and low doses of dragon's blood gel all promote healing in scalded mouse skin, with statistically significant differences compared to the control group. LingliGao, through collecting clinical data, concluded that dragon's blood capsules have a very good effect on promoting wound healing and cell regeneration, while also alleviating the patient's inflammatory response, sufficiently demonstrating that dragon's blood has excellent tissue-regenerating, wound-healing, and anti-inflammatory effects in clinical application. Furthermore, dragon's blood also plays an important role in reducing pigmentation caused by scalds. Liu Pingping et al., through biochemical and cellular experiments, detected the whitening, antioxidant, and anti-aging effects of dragon's blood in the skincare field. The results showed that dragon's blood has good whitening, antioxidant, and anti-aging effects, and has the potential to become a raw material for functional cosmetics. Therefore, this invention aims to increase the amount of dragon's blood in the existing technical solution, thereby increasing its overall proportion in the formula. In the treatment of scars caused by burns, topical application of traditional Chinese medicine with blood-activating and stasis-removing properties is the primary approach. Existing technologies heavily utilize Centella asiatica, a commonly used herb in the cosmetics industry. Centella asiatica is cold in nature, bitter and pungent in taste, and possesses effects such as clearing heat and dampness, reducing swelling and detoxifying. Modern medical research has revealed the complex pharmacological effects of active ingredients in Centella asiatica, including asiaticoside, hydroxyasiaticoside, and asiaticoside, in the field of skin diseases, especially in the healing of skin scars and wounds. Aloe vera has antibacterial and anti-inflammatory effects, promotes wound tissue regeneration, and enhances immunity. It can not only reduce wound pain and the rate of post-healing scar formation, but also has a therapeutic effect comparable to 3M artificial skin at a lower price, making it more suitable for daily use. Secondly, in existing burn ointment formulas, the medicinal components for relieving skin wound infection caused by burns are not emphasized. Therefore, this invention aims to add the use of honeysuckle, which has antibacterial effects, and Centella asiatica, which has anti-inflammatory effects, based on existing technologies. Modern pharmacological studies have shown that honeysuckle, as a traditional Chinese medicine for "clearing heat and detoxifying" and a classic food-medicine homology resource, possesses bioactivities such as anti-inflammatory, antibacterial, antioxidant, and immune-enhancing effects, thereby alleviating symptoms of inflammation and infection caused by burns and improving local immunity. In terms of processing, the effective components of dragon's blood, centella asiatica, and honeysuckle for treating burn wounds are all ethanol extracts, providing ample theoretical support for process optimization. Studies by Liu Huihui et al. have shown that the ethanol extract of dragon's blood promotes wound healing and has significant clinical application value. Lei Fang's ethanol extraction of centella asiatica demonstrated that the ethanol extract of its active components has the best effect, and a spray device for treating burns was prepared. The ethanol extract of honeysuckle and its effective component isoquercitrin reduce skin damage through antioxidant, anti-inflammatory, and anti-apoptotic effects. Therefore, process optimization can reduce interference from unnecessary effective components, greatly increasing efficacy and medication adherence.

[0017] Based on the aforementioned scientific theories, the purpose of this invention is to provide a traditional Chinese medicine composition with clear efficacy for the treatment of acute and prognostic burns. By weight, it comprises the following components: 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, 10-20 parts of honeysuckle, 15-25 parts of aloe vera, and 10-30 parts of borneol. This traditional Chinese medicine composition of the invention possesses significant functions in promoting skin tissue growth, anti-inflammatory and anti-infective properties, and inhibiting scar hyperplasia. This formula uses dragon's blood as the principal ingredient, primarily for promoting blood circulation and tissue regeneration; centella asiatica, which promotes blood circulation and removes blood stasis, and enhances the tissue regeneration and wound healing effects of dragon's blood, serves as the assistant ingredient; honeysuckle, which clears heat and detoxifies, and has antibacterial and anti-inflammatory properties, serves as the adjuvant ingredient; and aloe vera, which guides the other ingredients and harmonizes their effects, serves as the guiding ingredient, enhancing the overall efficacy of the formula. This formula focuses on "promoting blood circulation and tissue regeneration, clearing heat and detoxifying," while also considering antibacterial, anti-inflammatory, and repair-promoting effects. It aligns with the traditional Chinese medicine principle of "removing necrotic tissue and promoting new tissue growth" in burn treatment. Furthermore, it incorporates modern pharmacological research, demonstrating significant clinical value and addressing issues such as incomplete efficacy and complex composition, providing a more effective traditional Chinese medicine composition for burns.

[0018] This invention provides a traditional Chinese medicine composition for treating burns, comprising the following components by weight: 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, 10-20 parts of honeysuckle, 15-25 parts of aloe vera, and 10-30 parts of borneol. The preparation method of the traditional Chinese medicine composition for treating burns is as follows: alcohol extracts of dragon's blood, centella asiatica, and honeysuckle are extracted using an ethanol reflux extraction method; aloe vera and the alcohol extracts from the previous step are added to a matrix; after the matrix cools down, borneol is added to obtain the traditional Chinese medicine composition for treating burns.

[0019] In this invention, preferably, the traditional Chinese medicine composition for treating burns is composed of the following components by weight: 30-40 parts of dragon's blood, 30-35 parts of centella asiatica, 10-15 parts of honeysuckle, 15-20 parts of aloe vera, and 25-30 parts of borneol.

[0020] In this invention, more preferably, the traditional Chinese medicine composition for treating burns is composed of the following components by weight: 40 parts of dragon's blood, 30 parts of centella asiatica, 10 parts of honeysuckle, 15 parts of aloe vera, and 25 parts of borneol.

[0021] In this invention, preferably, the matrix is ​​one or more of petrolatum, sesame oil, and lanolin.

[0022] This invention also provides a method for preparing a traditional Chinese medicine composition for treating burns, comprising the following steps: Take 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, and 10-20 parts of honeysuckle. Add 70%-90% ethanol to extract for 1.0-2.0 hours, according to 6-8 times the total weight of dragon's blood, centella asiatica, and honeysuckle. Filter to remove residue, collect the decoction, concentrate the decoction at 70-90℃ until there is no alcohol taste, dry the concentrate under reduced pressure at 60-70℃, and pulverize it into fine powder. Grind 15-25 parts of aloe vera into a fine powder, and add it to the base along with the dry ointment powder obtained in the previous step, while stirring. Once the matrix has cooled to 55°C, add 10-30 parts of borneol while stirring until it is completely dissolved, thus obtaining the traditional Chinese medicine composition for treating burns.

[0023] In this invention, preferably, the preparation method of the traditional Chinese medicine composition for treating burns includes the following steps: Take 40 parts of dragon's blood, 30 parts of centella asiatica, and 10 parts of honeysuckle. Add 70% ethanol to extract for 1.0 h at 8 times the total weight of dragon's blood, centella asiatica, and honeysuckle. Filter to remove residue, collect the decoction, concentrate the decoction at 90℃ until there is no alcohol taste, dry the concentrate under reduced pressure at 70℃, and pulverize it into fine powder. Grind 15 parts of aloe vera into a fine powder and add it to the base along with the dry ointment powder obtained in the previous step, stirring as you add it. Once the matrix has cooled to 55°C, add 25 parts of borneol while stirring until it is completely dissolved to obtain the traditional Chinese medicine composition for treating burns.

[0024] The present invention also provides an application of the traditional Chinese medicine composition of the present invention in the preparation of anti-inflammatory or analgesic drugs.

[0025] The present invention also provides an application of the traditional Chinese medicine composition of the present invention in the preparation of a medicine for treating burns.

[0026] The technical solution of the present invention will be specifically described below through embodiments. Obviously, the described embodiments are not all the embodiments.

[0027] Example 1: Weigh out 30g of dragon's blood, 30g of centella asiatica, and 10g of honeysuckle. Add 6 times the total weight of dragon's blood, centella asiatica, and honeysuckle to reflux with 70% ethanol for 1.0h. Filter to remove residue and collect the decoction. Concentrate at 80℃ until there is no alcohol taste. Dry the concentrate under reduced pressure at 60℃ and pulverize it into a fine powder. Pulverize 20g of aloe vera into a fine powder and slowly pour it and the concentrate into 300g of completely melted petroleum jelly while stirring until completely mixed. When the oil temperature drops to 55℃, add 20g of borneol until completely dissolved to obtain the traditional Chinese medicine composition for treating burns in Example 1.

[0028] Example 2: Weigh 20g of dragon's blood, 40g of centella asiatica, and 15g of honeysuckle. Add 8 times the total weight of the three herbs to 90% ethanol and reflux for 2.0h. Filter to remove residue and collect the decoction. Concentrate at 90℃ until no alcohol odor remains. Dry the concentrate under reduced pressure at 60℃ and pulverize into a fine powder. Pulverize 15g of aloe vera into a fine powder and add it together with the dry ointment powder obtained in the previous step to 300g of sesame oil, stirring constantly until fully combined. When the oil temperature drops to 55℃, add 15g of borneol while stirring until completely dissolved to obtain the traditional Chinese medicine composition for treating burns described in Example 2.

[0029] Example 3: Weigh out 40g of dragon's blood, 20g of centella asiatica, and 20g of honeysuckle. Add 8 times the total weight of the three herbs to 80% ethanol and reflux for 1.5 hours. Filter to remove residue and collect the decoction. Concentrate at 90℃ until no alcohol odor remains. Dry the concentrate under reduced pressure at 65℃ and pulverize into a fine powder. Pulverize 15g of aloe vera into a fine powder and add it together with the dry ointment powder obtained in the previous step to 300g of lanolin while stirring until completely mixed. When the oil temperature drops to 55℃, add 10g of borneol while stirring until completely dissolved to obtain the traditional Chinese medicine composition for treating burns described in Example 3.

[0030] Example 4: Weigh 25g of dragon's blood, 35g of centella asiatica, and 16g of honeysuckle. Add 6 times the total weight of the three herbs to 90% ethanol and reflux for 2.0h. Filter to remove residue and collect the decoction. Concentrate at 70℃ until no alcohol odor remains. Dry the concentrate under reduced pressure at 70℃ and pulverize into a fine powder. Pulverize 15g of aloe vera into a fine powder and add it together with the dry ointment powder obtained in the previous step to 300g of sesame oil while stirring. When the oil temperature drops to 55℃, add 30g of borneol while stirring until completely dissolved. Dispense into sterile sprayers to obtain the traditional Chinese medicine composition for treating burns described in Example 4.

[0031] Example 5: Dragon's blood 40g, Centella asiatica 30g, honeysuckle 10g, add 8 times the total weight of dragon's blood, centella asiatica, and honeysuckle to reflux with 70% ethanol for 1.0h, filter to remove residue, collect the decoction; concentrate at 90℃ until no alcohol odor remains; dry the concentrate under reduced pressure at 70℃ and pulverize into a fine powder. Aloe vera 15g is pulverized into a fine powder and added together with the dry ointment powder obtained in the previous step to 300g of sesame oil while stirring; when the oil temperature drops to 55℃, add 25g of borneol while stirring until completely dissolved, filter, and obtain the traditional Chinese medicine composition for treating burns in Example 5.

[0032] Comparative Example 1: The burn ointment is manufactured by Xiuzheng Pharmaceutical Group (Hunan) Pharmaceutical Co., Ltd.

[0033] Comparative Example 2: Weigh out 30g of Centella asiatica and 10g of honeysuckle, grind them into coarse powder, add them together to sesame oil, mix well, soak for 24 hours, then slowly raise the temperature to 120℃ and keep it warm for 35 minutes. Filter while hot. When the filtrate cools down to 80℃, add 40g of fine powder of dragon's blood and 20g of aloe vera while stirring. When the oil temperature drops to about 55℃, add 20g of borneol while stirring until it is completely dissolved. This gives you the product of Comparative Example 2.

[0034] Pharmacodynamic experiments: 1. Studies on anti-inflammatory and analgesic effects: 1.1 Experimental Methods: One hundred and ninety-two SPF-grade KM mice were used and divided into three batches of 64 mice each. Each batch of mice was randomly divided into a model group, a control group 1, a control group 2, and groups 1-5, with eight mice in each group. Twenty-four hours before the experiment, the mice were depilated on their abdomens with an 8% sodium sulfide aqueous solution to a diameter of 3 cm. 0.2 g of the corresponding drug was applied to the depilated area twice a day for three days (groups 1-5 were treated with the traditional Chinese medicine composition prepared in Examples 1-5, and control groups 1 and 2 were treated with the product of control groups 1 and 2, respectively). The model group was not treated. After the drug administration was completed, the three batches of mice underwent xylene ear swelling test, acetic acid writhing test, and hot plate test to observe the pain threshold.

[0035] 1.2 Effects on ear swelling, number of writhing episodes, and pain threshold in mice after burns.

[0036] Compared with the model group, the ear swelling and number of writhing movements in each treatment group were significantly reduced (P < 0.001). Compared with Comparative Example 1, the ear swelling and number of writhing movements in Examples 1-5 were significantly reduced (P < 0.05). At 0 h post-scalding, the pain threshold in each treatment group changed compared with the model group, but there was no significant difference. At 1 h and 7 h post-scalding, the pain threshold in the treatment groups was significantly increased compared with the model group (P < 0.001). Compared with Comparative Example 1, the pain threshold in Examples 1-5 was significantly increased (P < 0.05). Among the five examples, only Example 5 was superior to the comparative examples. The results are shown in Tables 1 and 2.

[0037] Table 1 Comparison of ear swelling and number of acetic acid-induced writhing episodes in mice of different groups ( (n=8) Note: Compared with the model group, △△△ P < 0.001, △△ P < 0.01; compared with Comparative Example 1, <0.05; compared with the two control groups, # P < 0.05 Table 2 Comparison of pain thresholds in mice of different groups ( (n=8) Note: Compared with the model group, △△△ P < 0.001, △△ P < 0.01, △ P < 0.05; compared with Comparative Example 1, <0.05; compared with the two control groups, # P < 0.05.

[0038] 2. Burn effect test: 2.1 Experimental Methods: Two hundred and fifty-six SD rats, weighing 180–230 g, were used. One day prior to the back scalding experiment, the rats were shaved with 10% barium sulfide and anesthetized intraperitoneally with 1% sodium pentobarbital (40 mg / kg). A 3 cm diameter iron rod was placed in boiling water for 15 minutes, then quickly dried with gauze. One end was then pressed tightly against the rat's back skin for 7 seconds, creating two scalding wounds on each rat's back. Immediately afterward, 5 mL of lactated Ringer's solution was injected intraperitoneally to combat shock. Thirty minutes after the scalding, the scalded rats were housed individually and randomly divided into eight groups: a model group, control group 1, control group 2, and groups 1–5 (Examples 1–5), with 32 rats in each group. They were kept in an environment with a room temperature of 25°C and 50% humidity, with free access to food and water. In each treatment group, 0.5g / rat of the corresponding drug was applied to the burn site (groups 1-5 were treated with the traditional Chinese medicine composition prepared in Examples 1-5, and Comparative Examples 1 and 2 were treated with the product of Comparative Examples 1 and 2, respectively), once daily. The treatment site was bandaged with sterile gauze for 21 consecutive days. At 5, 10, and 15 days post-burn, 8 rats from each group were taken, and the longitudinal and transverse lengths of the wound were measured using calipers. The area of ​​the scab was calculated using the formula for the area of ​​an ellipse (S=π×a×b, where π is pi, and a and b are half the longitudinal and transverse lengths of the wound, respectively). The time for scab removal and healing of the burn wound in each group was observed and recorded. At 1 and 2 days post-burn, 8 rats from each group were taken, and serum TNF-α and IL-1 levels were measured using ELISA. At 1, 2, and 7 days post-burn, 8 rats from each group were taken, and serum SOD and MDA expression levels were measured using ELISA.

[0039] 2.2 Effects on scab formation area and scab removal time of scalded wounds in rats: On day 5 post-scald, there was no statistically significant difference in the scab area of ​​the scald wounds among the groups. Compared with the model group, the scab area of ​​the wounds in each medication group gradually decreased over time, and the scab area and healing time on days 10 and 15 post-treatment were significantly shortened (P<0.001). Compared with control group 1, the scab area and healing time of the wounds in Examples 1-5 were significantly reduced (P<0.05). Among the five examples, only Example 5 was superior to the control groups; specific results are shown in Table 3.

[0040] Table 3. Effects on scab formation area and scab removal time of scalded wounds in rats ( (n=8) Note: Compared with the model group, △△△ P < 0.001, △△ P < 0.01; compared with Comparative Example 1, <0.05; compared with the two control groups, # P < 0.05.

[0041] 2.2 Effects on serum MDA content and SOD activity in scalded rats: On day 1 post-scalding, compared with the model group, the serum MDA content of scalded rats in each treatment group was reduced and the SOD activity was increased, but the differences were not statistically significant. As time progressed, the serum MDA content in each treatment group gradually decreased, while the serum SOD activity gradually increased, reaching a peak on day 7. On day 2 post-scalding, compared with the model group, the MDA content in each treatment group was significantly reduced (P < 0.001), and the serum SOD activity was significantly increased (P < 0.001). Compared with Comparative Example 1, the serum MDA content in Examples 1-5 was significantly reduced, and the SOD activity was increased (P < 0.05). On day 7 post-scalding, compared with the model group, the serum MDA content in each treatment group further decreased (P < 0.001), and the serum SOD activity further increased (P < 0.001). Compared with groups 1-5, the serum MDA content in group 1 was significantly lower and the SOD activity was significantly increased (P<0.05). Among the 5 groups, only group 5 was better than the comparative groups. The specific results are shown in Tables 4 and 5.

[0042] Table 4. Effects of serum MDA levels on scalded rats at different time points ( (n=8)

[0043] Note: Compared with the model group, △△△ P < 0.001; compared with Comparative Example 1, <0.05; compared with the two control groups, # P < 0.05.

[0044] Table 5. Effects of serum SOD activity on scalded rats at different time points ( (n=8)

[0045] Note: Compared with the model group, △△△ P < 0.001; compared with Comparative Example 1, <0.05; compared with the two control groups, ## P < 0.01,# P < 0.05.

[0046] 2.3 Effects of compound burn ointment on serum TNF-α and IL-1 levels in scalded rats: On days 1 and 2 after the burn, the serum levels of TNF-α and IL-1 in rats in each treatment group were significantly lower than those in the model group (P<0.001, P<0.01). Compared with control group 1, the serum levels of TNF-α and IL-1 in rats in Examples 1-5 were significantly lower (P<0.01, P<0.001). Among the five groups, only Example 5 was better than the control groups (see Tables 6 and 7).

[0047] Table 6. Effects of TNF-α levels in serum of scalded rats at different time points ( (n=8)

[0048] Note: Compared with the model group, △△△ P < 0.001; compared with Comparative Example 1, <0.01, <0.05, compared with the control group 2, # P < 0.05 Table 7. Effects of serum IL-1 activity at different time points in scalded rats ( (n=8)

[0049] Note: Compared with the model group, △△△ P < 0.001; compared with Comparative Example 1, <0.01, <0.05; compared with the two control groups, # P < 0.05 Based on the above data, it was found that Example 5 of the present invention significantly reduced ear swelling and writhing frequency in mice compared to other example groups, inhibited inflammatory response, and increased pain threshold; both scab formation time and scab area were reduced, showing a good protective effect on burn wounds. Simultaneously, it effectively reduced the MDA content in rat serum while increasing SOD activity, indicating its ability to resist post-burn oxidative stress. After burns, Example 5 significantly reduced the levels of TNF-α and IL-1 in serum, indicating its ability to inhibit the release of inflammatory factors TNF-α and IL-1, reduce local inflammatory response after trauma, and promote wound healing. Among the five example groups, Example 5 showed the best efficacy, superior to the comparative groups.

[0050] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A traditional Chinese medicine composition for treating burns, characterized in that, By weight, it consists of the following components: 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, 10-20 parts of honeysuckle, 15-25 parts of aloe vera, and 10-30 parts of borneol. The preparation method of the traditional Chinese medicine composition for treating burns is as follows: The ethanol extracts of dragon's blood, centella asiatica and honeysuckle were extracted by ethanol reflux extraction. Add the aloe vera and the alcohol extract from the previous step to the matrix together; After the matrix cools down, borneol is added to obtain the traditional Chinese medicine composition for treating burns.

2. The traditional Chinese medicine composition for treating burns according to claim 1, characterized in that, By weight, it consists of the following components: 30-40 parts of dragon's blood, 30-35 parts of centella asiatica, 10-15 parts of honeysuckle, 15-20 parts of aloe vera, and 25-30 parts of borneol.

3. The traditional Chinese medicine composition for treating burns according to claim 1, characterized in that, By weight, it consists of the following components: 40 parts dragon's blood, 30 parts centella asiatica, 10 parts honeysuckle, 15 parts aloe vera, and 25 parts borneol.

4. The traditional Chinese medicine composition for treating burns according to claim 1, characterized in that, The matrix is ​​one or more of petrolatum, sesame oil, and lanolin.

5. A method for preparing a traditional Chinese medicine composition for treating burns, characterized in that, Includes the following steps: Take 20-40 parts of dragon's blood, 20-40 parts of centella asiatica, and 10-20 parts of honeysuckle. Add 70%-90% ethanol and reflux for 1.0-2.0 h, according to 6-8 times the total weight of dragon's blood, centella asiatica, and honeysuckle. Filter to remove residue, collect the decoction, concentrate the decoction at 70-90℃ until there is no alcohol taste, dry the concentrate under reduced pressure at 60-70℃, and pulverize it into fine powder. Grind 15-25 parts of aloe vera into a fine powder, and add it to the base along with the dry ointment powder obtained in the previous step, while stirring. Once the matrix has cooled to 55°C, add 10-30 parts of borneol while stirring until it is completely dissolved, thus obtaining the traditional Chinese medicine composition for treating burns.

6. The method for preparing the traditional Chinese medicine composition for treating burns according to claim 5, characterized in that, The preparation method of the traditional Chinese medicine composition for treating burns includes the following steps: Take 40 parts of dragon's blood, 30 parts of centella asiatica, and 10 parts of honeysuckle. Add 70% ethanol to extract for 1.0 h at 8 times the total weight of dragon's blood, centella asiatica, and honeysuckle. Filter to remove residue, collect the decoction, concentrate the decoction at 90℃ until there is no alcohol taste, dry the concentrate under reduced pressure at 70℃, and pulverize it into fine powder. Grind 15 parts of aloe vera into a fine powder and add it to the base along with the dry ointment powder obtained in the previous step, stirring as you add it. Once the matrix has cooled to 55°C, add 25 parts of borneol while stirring until it is completely dissolved to obtain the traditional Chinese medicine composition for treating burns.

7. The use of a traditional Chinese medicine composition according to any one of claims 1-4 in the preparation of anti-inflammatory or analgesic drugs.

8. The use of the traditional Chinese medicine composition according to any one of claims 1-4 in the preparation of a scald treatment drug.

Images