Iron porphyrin hydrogen-bonded organic framework nanosenzyme and preparation method and application thereof

By introducing a tetracycline nucleic acid aptamer into an iron porphyrin hydrogen-bonded organic framework nanozyme, Apt-Au/FeHOF was constructed, which solved the problem of poor selectivity in existing nanozyme detection technologies and achieved highly selective and rapid colorimetric detection of tetracycline.

CN122238633APending Publication Date: 2026-06-19HEBEI PROVINCIAL INST OF PROD QUALITY SUPERVISION & INSPECTION
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEBEI PROVINCIAL INST OF PROD QUALITY SUPERVISION & INSPECTION
Filing Date
2026-03-26
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing nanozyme detection technologies are not highly selective for tetracycline antibiotics, making rapid and convenient detection difficult. Furthermore, they can interfere with structurally similar substances such as oxytetracycline and chlortetracycline.

Method used

By introducing a tetracycline nucleic acid aptamer into an iron porphyrin hydrogen-bonded organic framework nanozyme and combining it with gold nanoparticles to form a porous framework material through hydrogen bond self-assembly, a highly selective detection of tetracycline was achieved.

Benefits of technology

It achieves rapid detection of tetracycline with high sensitivity and selectivity, and can catalyze the colorimetric development of the substrate in the presence of H2O2. The tetracycline content is detected by colorimetry, with good linearity and selectivity, and is suitable for rapid and visual detection.

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Abstract

This invention relates to the field of tetracycline detection technology, specifically disclosing an iron porphyrin hydrogen-bonded organic framework nanozyme, its preparation method, and its applications. The iron porphyrin hydrogen-bonded organic framework nanozyme comprises an iron porphyrin hydrogen-bonded organic framework, gold nanoparticles, and a tetracycline nucleic acid aptamer. The gold nanoparticles are non-covalently loaded onto the surface and pores of the iron porphyrin hydrogen-bonded organic framework, and the tetracycline nucleic acid aptamer and gold nanoparticles are connected by coordination covalent bonds. The iron porphyrin hydrogen-bonded organic framework nanozyme exhibits peroxide-like nanozyme activity, catalyzing the oxidation of the chromogenic substrate TMB in the presence of H₂O₂ to detect tetracycline content. By introducing the hydrogen-bonded organic framework and the tetracycline nucleic acid aptamer, the detection specificity is improved, achieving highly sensitive, highly selective, and rapid detection of tetracycline. The colorimetric sensor constructed based on the iron porphyrin hydrogen-bonded organic framework nanozyme provides a new method for rapid and visual detection of tetracycline in feed.
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