A method for preparing a photobiological photoreactor

Abstract

The present application relates to the technical field of spirulina culture, and discloses a preparation method of a photobiological photoreactor, which comprises the following steps: arranging a carbon dioxide sensor and an oxygen sensor in a transparent tank to collect a carbon dioxide concentration difference and an oxygen concentration difference in the transparent tank through the carbon dioxide sensor and the oxygen sensor; connecting an edge computing module with the carbon dioxide sensor and the oxygen sensor respectively to calculate a preset carboxylation enzyme carboxy oxygen ratio of spirulina in the transparent tank through the carbon dioxide concentration difference and the oxygen concentration difference; connecting the edge computing module with a growth control module in the transparent tank to configure an instruction generation function of the edge computing module for converting the preset carboxylation enzyme carboxy oxygen ratio into a regulation instruction of the growth control module; and after the transparent tank, the carbon dioxide sensor, the oxygen sensor, the edge computing module, the growth control module and the instruction generation function are deployed, the preparation of the photobiological photoreactor is realized. The present application can meet the real photosynthetic metabolism demand of spirulina.

Description

Technical Field

[0001] This invention relates to a method for preparing a photobioreactor, belonging to the field of spirulina cultivation technology. Background Technology

[0002] In the field of spirulina cultivation, especially in the large-scale production process of spirulina cultivation in photobioreactors, spirulina is sensitive to photosynthetic metabolism and its growth status is easily affected by the environment. This leads to low photosynthetic efficiency and unstable growth rate under processes such as light regulation, carbon source supply, and temperature control. To solve the above problems, the existing technology generally adopts the method of collecting environmental parameters for threshold regulation, that is, monitoring and adjusting the cultivation environment in the photobioreactor to adapt to the growth of spirulina.

[0003] Currently, the methods of controlling environmental parameters have a significant impact on the cultivation effect of spirulina. In existing technologies, technicians typically rely on their cultivation experience to determine the appropriate range of environmental parameters by observing the growth status of spirulina, and then manually set control thresholds. These parameters are then adjusted through multiple trials and errors until the growth status of spirulina improves. In addition, some existing technologies introduce simple environmental parameter linkage control methods to reduce the number of trials. However, the design logic of these existing technologies still focuses on ensuring that environmental parameters reach the preset thresholds, paying more attention to the achievement of environmental parameter standards, and rarely considering the matching degree between parameter control and the actual photosynthetic metabolic state of spirulina. For example, setting a carbon dioxide concentration of 5%, and stopping the adjustment of carbon dioxide once it reaches 5%, may not be the actual requirement of spirulina at that moment. At a certain growth stage, spirulina may actually require 4% carbon dioxide; even if the parameter reaches 5%, it does not meet its true photosynthetic metabolic needs.

[0004] Therefore, existing biophotoreactors cannot meet the actual photosynthetic metabolic needs of spirulina. Summary of the Invention

[0005] This invention provides a method for preparing a photobioreactor, the main purpose of which is to meet the actual photosynthetic metabolic needs of spirulina.

[0006] To achieve the above objectives, the present invention provides a method for preparing a photobiophotoreactor, comprising:

[0007] A carbon dioxide sensor and an oxygen sensor are installed in a transparent tank to collect the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank.

[0008] The edge computing module is connected to the carbon dioxide sensor and the oxygen sensor respectively, so as to calculate the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank by the difference between the carbon dioxide concentration and the difference between the oxygen concentration;

[0009] The edge computing module is connected to the growth control module in the transparent tank to configure the edge computing module to generate instructions that convert the preset carboxylase carboxyl-oxygen ratio into the regulation instructions of the growth control module.

[0010] The growth control module includes a light module, a temperature control component, a water flow drive component, and a carbon dioxide replenishment valve.

[0011] After deploying the transparent tank, the carbon dioxide sensor, the oxygen sensor, the edge computing module, the growth control module, and the instruction generation function, the photobiophotoreactor is prepared.

[0012] Optionally, the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank is calculated using the difference in carbon dioxide concentration and the difference in oxygen concentration, including:

[0013] Query the baseline respiratory rate of the spirulina;

[0014] The preset carboxyl-oxygen ratio of the spirulina is calculated based on the carbon dioxide concentration difference, the oxygen concentration difference, and the basal respiration rate.

[0015] Optionally, query the basal respiratory rate of the spirulina, including:

[0016] The basic respiration rate of Spirulina under standard culture conditions is pre-stored in the edge computing module;

[0017] The basic respiration rate of the spirulina is queried in the edge computing module.

[0018] Optionally, the preset carboxylase carboxyl-oxygen ratio of the spirulina is calculated based on the carbon dioxide concentration difference, the oxygen concentration difference, and the basal respiration rate, including:

[0019] The preset carboxyl-oxygen ratio of the spirulina was calculated using the following formula:

[0020]

[0021] in, This indicates the preset carboxyl-oxygen ratio of the carboxylase. This represents the difference in carbon dioxide concentration. This represents the difference in oxygen concentration. This indicates the basal respiratory rate.

[0022] Optionally, the edge computing module is connected to the carbon dioxide sensor and the oxygen sensor respectively, including:

[0023] The output lines of the carbon dioxide and oxygen sensors are crimped to the analog input terminals of the edge computing module to complete the physical connection.

[0024] Optionally, carbon dioxide and oxygen sensors are installed inside the transparent tank, including:

[0025] Through holes are made in the tank wall to insert carbon dioxide sensors and oxygen sensors respectively.

[0026] Optionally, the transparent tank is insulated with graphene and foam.

[0027] Optionally, the carbon dioxide concentration difference and oxygen concentration difference in the transparent tank are collected by the carbon dioxide sensor and the oxygen sensor, including:

[0028] The carbon dioxide sensor simultaneously collects the concentrations of carbon dioxide in the top gas phase and the bottom liquid phase.

[0029] The carbon dioxide concentration difference is obtained by subtracting the carbon dioxide concentration in the bottom liquid phase from the top gas phase concentration.

[0030] The oxygen sensor simultaneously collects the oxygen concentration in the top gas phase and the oxygen concentration in the bottom liquid phase.

[0031] The oxygen concentration difference is obtained by subtracting the oxygen concentration in the liquid phase from the oxygen concentration in the top gas phase.

[0032] Optionally, connecting the edge computing module to the growth control module in the transparent tank includes:

[0033] Connect the output terminals of the edge computing module to the corresponding control ports of the illumination module, temperature control component, water flow drive component, and carbon dioxide replenishment valve using shielded cables, so as to connect the edge computing module to the growth control module in the transparent tank.

[0034] Optionally, the edge computing module is configured with an instruction generation function that converts the preset carboxylase carboxyl-oxygen ratio into a regulation instruction for the growth control module, including:

[0035] The edge computing module incorporates a preset optimal range of carboxyl-oxygen ratio for spirulina growth.

[0036] Configure the threshold comparison logic of the edge computing module, wherein the threshold comparison logic includes:

[0037] When the preset carboxylase carboxyl-oxygen ratio is lower than the preset optimal range of carboxylase carboxyl-oxygen ratio, a regulatory instruction to inhibit photorespiration and promote carboxylation reaction is generated; when the preset carboxylase carboxyl-oxygen ratio is higher than the preset optimal range of carboxylase carboxyl-oxygen ratio, a regulatory instruction to optimize carbon source supply and improve oxygen release efficiency is generated.

[0038] Establish an instruction output protocol for the edge computing module so that the generated control instructions match the communication interface of the growth control module, and then complete the configuration of the instruction generation function.

[0039] Optionally, the fabrication of a photobiophotoreactor includes:

[0040] The transparent tank, carbon dioxide sensor, oxygen sensor, edge computing module, growth control module and instruction generation function are assembled into a special test fixture to introduce standard air into the transparent tank.

[0041] Under standard air conditions, check whether the concentration deviation between the carbon dioxide sensor and the oxygen sensor is within the standard range;

[0042] When the concentration deviation between the carbon dioxide sensor and the oxygen sensor is within the standard range, a water pressure holding test is performed on the transparent tank to confirm that there is no leakage in the transparent tank before the photobiophotoreactor is prepared.

[0043] Compared to the problems described in the background art, this embodiment of the invention monitors the activity of a preset carboxylase by installing a carbon dioxide sensor and an oxygen sensor in a transparent tank. Since directly monitoring the activity of the preset carboxylase is difficult, this indirect monitoring method can be used. Furthermore, this embodiment uses graphene and foam insulation to maintain a stable temperature inside the transparent tank. Further, this embodiment uses the carbon dioxide sensor and the oxygen sensor to collect the difference in carbon dioxide concentration and the difference in oxygen concentration within the transparent tank, and calculates the subsequent activity of the preset carboxylase based on the concentration changes. This embodiment connects an edge computing module to the carbon dioxide sensor and the oxygen sensor respectively to... The concentration data is transmitted to the edge computing module, which performs the specific calculations. Further, in this embodiment, the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent container is calculated using the difference between the carbon dioxide and oxygen concentrations. This preset carboxylase carboxyl-oxygen ratio represents the activity of the preset carboxylase, thereby quantifying the actual photosynthetic metabolic requirements of the preset carboxylase. Furthermore, this embodiment configures the edge computing module to convert the preset carboxylase carboxyl-oxygen ratio into a control command generation function for the growth control module. This allows the preset carboxylase carboxyl-oxygen ratio to reflect the activity of the preset carboxylase, and the activity of the preset carboxylase is used to control various environmental parameters, thereby ensuring that the subsequently prepared biophotoreactor meets the actual photosynthetic metabolic requirements of spirulina. Therefore, this invention can meet the actual photosynthetic metabolic requirements of spirulina. Attached Figure Description

[0044] Figure 1 A schematic flowchart of a method for preparing a photobiophotoreactor according to an embodiment of the present invention;

[0045] Figure 2 This is a schematic diagram of a conventional threshold control method for a method of preparing a photobioreactor according to an embodiment of the present invention;

[0046] Figure 3 A schematic diagram of the Ethio culture medium used in a method for preparing a photobioreactor according to an embodiment of the present invention;

[0047] Figure 4 This is an explosive assembly diagram of a photobioreactor prepared according to an embodiment of the present invention.

[0048] Figure 5 A schematic diagram of a photobioreactor provided in an embodiment of the present invention;

[0049] Figure 6This is a multi-view engineering schematic diagram of a photobioreactor prepared according to an embodiment of the present invention.

[0050] Figure 7 A functional block diagram of a photobiophotoreactor preparation system provided in an embodiment of the present invention;

[0051] Figure 8 A schematic diagram of a computer device for a method of preparing a photobioreactor according to an embodiment of the present invention;

[0052] The objectives, features, and advantages of this invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0053] It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0054] This application provides a method for preparing a photobiophotoreactor. The execution subject of this method includes, but is not limited to, at least one electronic device configured to execute the method provided in this application, such as a server or a terminal. In other words, the method for preparing the photobiophotoreactor can be executed by software or hardware installed on a terminal device or a server device. The server includes, but is not limited to, a single server, a server cluster, a cloud server, or a cloud server cluster.

[0055] Reference Figure 1 The diagram shown is a flowchart illustrating a method for preparing a photobioreactor according to an embodiment of the present invention. In this embodiment, the method for preparing the photobioreactor includes:

[0056] Reference Figure 2 The diagram shown illustrates a conventional threshold control method for a photobioreactor preparation method according to an embodiment of the present invention. Figure 2 This diagram shows the threshold management interface of a photobioreactor in existing technology. It typically reflects the design logic of traditional environmental parameter regulation, which takes achieving a fixed parameter range as the core regulation goal and presets environmental parameters such as temperature, light, pH, dissolved oxygen, carbon dioxide, and turbidity to fixed numerical threshold ranges.

[0057] S1. A carbon dioxide sensor and an oxygen sensor are installed in a transparent tank to collect the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank through the carbon dioxide sensor and the oxygen sensor.

[0058] This invention provides an embodiment of the invention that uses carbon dioxide and oxygen sensors installed in a transparent container to monitor the activity of a preset carboxylase. Since it is difficult to directly monitor the activity of the preset carboxylase, this indirect monitoring method can be used to monitor the activity of the preset carboxylase.

[0059] The oxygen sensor primarily reflects the oxygen concentration in the gas by detecting the oxygen content, while the carbon dioxide sensor calculates the carbon dioxide concentration in the gas by detecting the absorption characteristics of carbon dioxide to specific infrared light.

[0060] In one embodiment of the present invention, the step of setting a carbon dioxide sensor and an oxygen sensor in a transparent tank includes: opening through holes in the tank wall to insert the carbon dioxide sensor and the oxygen sensor respectively.

[0061] Furthermore, in this embodiment of the invention, graphene and foam insulation are used to maintain a stable temperature inside the transparent tank.

[0062] In one embodiment of the present invention, the transparent tank is insulated with graphene and foam.

[0063] Furthermore, in this embodiment of the invention, the carbon dioxide sensor and the oxygen sensor collect the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank, so as to calculate the activity of the subsequent preset carboxylase by means of the concentration change.

[0064] In one embodiment of the present invention, the step of collecting the carbon dioxide concentration difference and oxygen concentration difference in the transparent tank through the carbon dioxide sensor and the oxygen sensor includes: simultaneously collecting the top gas phase carbon dioxide concentration and the bottom liquid phase carbon dioxide concentration through the carbon dioxide sensor; subtracting the bottom liquid phase carbon dioxide concentration from the top gas phase carbon dioxide concentration to obtain the carbon dioxide concentration difference; simultaneously collecting the top gas phase oxygen concentration and the bottom liquid phase oxygen concentration through the oxygen sensor; subtracting the bottom liquid phase oxygen concentration from the top gas phase oxygen concentration to obtain the oxygen concentration difference.

[0065] It should be noted that the carbon dioxide sensor and oxygen sensor mentioned above are respectively equipped with gas phase detection type and liquid phase detection type.

[0066] S2. Connect the edge computing module to the carbon dioxide sensor and the oxygen sensor respectively, so as to calculate the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank through the difference in carbon dioxide concentration and the difference in oxygen concentration.

[0067] In this embodiment of the invention, the edge computing module is connected to the carbon dioxide sensor and the oxygen sensor respectively, so that the collected concentration data is transmitted to the edge computing module, which then performs the specific calculations.

[0068] The edge computing module is an embedded computing control unit deployed locally on the photobioreactor to collect and process various sensor data and perform calculations such as the carboxy-oxygen ratio.

[0069] In one embodiment of the present invention, connecting the edge computing module to the carbon dioxide sensor and the oxygen sensor respectively includes: crimping the output lines of the carbon dioxide sensor and the oxygen sensor to the analog input terminal of the edge computing module to complete the physical connection.

[0070] Furthermore, in this embodiment of the invention, the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent container is calculated by the difference between the carbon dioxide concentration and the difference between the oxygen concentration, so as to represent the activity of the preset carboxylase by the preset carboxylase carboxyl-oxygen ratio, thereby quantifying the actual photosynthetic metabolic requirements of the preset carboxylase.

[0071] Specifically, the preset carboxylase refers to ribulose-1,5-bisphosphate carboxylase, also known as Rubisco. Rubisco is a bifunctional enzyme that catalyzes both carboxylation and oxygenation reactions. When Rubisco combines with carbon dioxide, it forms an oxygenated product that effectively reduces photorespiration, increases photosynthetic efficiency, and rapidly promotes the growth and development of spirulina. However, when Rubisco combines with oxygen, it forms an oxygenated product that causes photorespiration, leading to carbon loss and thus halting the growth of spirulina.

[0072] In one embodiment of the present invention, the step of calculating the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank by means of the difference in carbon dioxide concentration and the difference in oxygen concentration includes: querying the basic respiration rate of the spirulina; and calculating the preset carboxylase carboxyl-oxygen ratio of the spirulina based on the difference in carbon dioxide concentration, the difference in oxygen concentration, and the basic respiration rate.

[0073] The basal respiration rate is the baseline value of the difference in carbon dioxide and oxygen concentration between the gas and liquid phases caused by basal respiration of Spirulina under standard culture conditions and in a non-photosynthetic state.

[0074] In another embodiment of the present invention, querying the basic respiration rate of the spirulina includes: pre-storing the basic respiration rate of the spirulina under standard culture conditions in an edge computing module; and querying the basic respiration rate of the spirulina in the edge computing module.

[0075] The standard culture environment refers to the standardized culture conditions required for calibrating the basic respiration rate of Spirulina. Specifically, it includes: pre-cleaning and sterilizing the photobioreactor; using Ethiocarbazin medium as the nutrient solution; controlling the pH of the nutrient solution at approximately 9-11; setting the culture temperature at 33-35℃; providing complete shading to block photosynthesis; using an aeration pump to ensure the algal solution flows at a speed of 4-6 cm to guarantee uniform contact with nutrients and discharge of metabolic products; maintaining a carbon dioxide concentration of 1200-1300 ppm and a dissolved oxygen concentration of 8.4-8.5 Mg / L; and ensuring the algal solution's optical density (OD value) is 0.1 at inoculation. Furthermore, it avoids environmental conditions where the dissolved oxygen concentration exceeds the carbon dioxide concentration, preventing the Rubisco enzyme from binding with oxygen. It should be noted that the specific parameters in these standard culture environments are suitable parameters determined by previous experiments and will not be elaborated upon here.

[0076] Reference Figure 3 The image shown is a schematic diagram of the Ethio culture medium used in a method for preparing a photobioreactor according to an embodiment of the present invention. Figure 3 The document contains a formula for Zaluk medium, which clearly lists the specific amounts of each reagent used when preparing 1L and 50L of medium. It can be used to guide the preparation of medium for Spirulina culture.

[0077] In another embodiment of the present invention, the step of calculating the preset carboxylase carboxyl-oxygen ratio of the spirulina based on the carbon dioxide concentration difference, the oxygen concentration difference, and the basal respiration rate includes: calculating the preset carboxylase carboxyl-oxygen ratio of the spirulina using the following formula:

[0078]

[0079] in, This indicates the preset carboxyl-oxygen ratio of the carboxylase. This represents the difference in carbon dioxide concentration. This represents the difference in oxygen concentration. This indicates the basal respiratory rate.

[0080] It should be noted that the preset carboxyl-oxygen ratio of carboxylase is essentially the ratio of the rate at which the enzyme catalyzes the carboxylation reaction to the rate at which it catalyzes the oxygenation reaction.

[0081] S3. Connect the edge computing module to the growth control module in the transparent tank to configure the edge computing module to generate instructions that convert the preset carboxylase carboxyl-oxygen ratio into the regulation instructions of the growth control module.

[0082] In one embodiment of the present invention, connecting the edge computing module to the growth control module in the transparent tank includes: connecting the output terminals of the edge computing module to the corresponding control ports of the illumination module, temperature control component, water flow drive component, and carbon dioxide replenishment valve respectively using shielded wires, so as to connect the edge computing module to the growth control module in the transparent tank.

[0083] Furthermore, in this embodiment of the invention, the edge computing module is configured to convert the preset carboxylase carboxyl-oxygen ratio into a control instruction of the growth control module. This allows the preset carboxylase carboxyl-oxygen ratio to reflect the activity of the preset carboxylase. By observing the activity of the preset carboxylase, various environmental parameters can be controlled, thereby enabling the subsequently prepared biophotoreactor to meet the actual photosynthetic metabolic needs of spirulina.

[0084] In one embodiment of the present invention, the instruction generation function of configuring the edge computing module to convert the preset carboxylase carboxy-oxygen ratio into a regulation instruction of the growth control module includes: embedding a preset optimal range of carboxylase carboxy-oxygen ratio corresponding to spirulina growth in the edge computing module; configuring a threshold comparison logic of the edge computing module, wherein the threshold comparison logic includes: generating a regulation instruction to inhibit photorespiration and promote carboxylation reaction when the preset carboxylase carboxy-oxygen ratio is lower than the preset optimal range of carboxylase carboxy-oxygen ratio, and generating a regulation instruction to optimize carbon source supply and improve oxygen release efficiency when the preset carboxylase carboxy-oxygen ratio is higher than the preset optimal range of carboxylase carboxy-oxygen ratio; and establishing an instruction output protocol of the edge computing module so that the generated regulation instruction matches the communication interface of the growth control module, thereby completing the configuration of the instruction generation function.

[0085] The instruction output protocol is a communication rule that enables the control instructions generated by the edge computing module to be received by the growth control module. The instruction generation function is actually the function of converting the preset carboxylase carboxyl-oxygen ratio into instructions.

[0086] It should be noted that the preset optimal range of carboxylase carboxyl-oxygen ratio is a numerical range calculated by Spirulina in a standard culture environment with added light. This means that the aforementioned standard culture environment is without light, while the culture here is to enable Spirulina to grow efficiently. Therefore, additional light is added here, while other parameters remain unchanged. The suitable light intensity for Spirulina growth is generally maintained at 20-400 lux.

[0087] Optionally, when the preset carboxylase carboxy-oxygen ratio is lower than the preset optimal range for carboxylase carboxy-oxygen ratio, it means that the preset carboxylase carboxy-oxygen ratio is lower than the lower limit of the preset optimal range for carboxylase carboxy-oxygen ratio. Further, the process of generating the regulatory command to inhibit photorespiration and promote carboxylation mainly includes increasing the carbon dioxide input, accelerating the algal liquid flow rate through an aeration pump to reduce the dissolved oxygen concentration in the liquid phase, moving the light intensity closer to a fixed range, and moving the temperature closer to a fixed range. Each adjustment is made according to the smallest unit. For example, if the smallest unit of these types of parameters is... 1. When increasing the carbon dioxide input, increase the carbon dioxide input by 1 unit. When accelerating the algal liquid flow rate through the aeration pump to reduce the dissolved oxygen concentration in the liquid phase, increase the flow rate by 1 unit. When moving the light intensity closer to a fixed range, if the standard light range is 20-400 lux, and the midpoint of 20-400 lux is B, and the current light intensity is greater than B, then decrease the current light intensity by 1 unit to move closer to B; otherwise, increase it by 1 unit to move closer to B. The principle of moving the temperature closer to a fixed range is similar to that of moving the light intensity closer to a fixed range, and will not be elaborated on here.

[0088] Optionally, when the preset carboxylase carboxy-oxygen ratio is higher than the preset optimal range for carboxylase carboxy-oxygen ratio, it means that the preset carboxylase carboxy-oxygen ratio is higher than the upper limit of the preset optimal range for carboxylase carboxy-oxygen ratio. Further, the process of generating the control command to optimize carbon source supply and improve oxygen release efficiency mainly includes reducing excessive carbon dioxide input, further accelerating the algal liquid flow rate through an aeration pump to improve oxygen release efficiency, maintaining light intensity within a fixed range, and maintaining temperature within a fixed range. Each adjustment is made according to the smallest unit. For example, if these types of parameters... The smallest unit is 1. Therefore, reducing the amount of carbon dioxide input means reducing the amount of carbon dioxide input by 1 unit. To further increase the flow rate of algal solution and thus improve oxygen release efficiency by using an aeration pump, the flow rate is increased by 1 unit. When maintaining the light intensity within a fixed range, if the standard light intensity range is 20-400 lux, and the midpoint of 20-400 lux is B, when the current light intensity deviates from B, it is adjusted by 1 unit each time in the direction of B to maintain stability within the range. The principle of maintaining the temperature within a fixed range is similar to that of maintaining the light intensity within a fixed range, and will not be elaborated on here.

[0089] S4. The growth control module includes a light module, a temperature control component, a water flow drive component, and a carbon dioxide replenishment valve.

[0090] In this embodiment of the invention, the light module is a dedicated lighting component that provides light intensity in the range of 20-400 lux for the photosynthetic growth of spirulina. The temperature control component is a regulating device that can maintain the spirulina cultivation temperature in the range of 33-35℃. The core achieves temperature stability through a graphene heating film and a foam insulation structure. The water flow driving component is a power component that can control the flow of algal solution at a speed of 4-6 cm / s, such as an air pump. By driving the flow of algal solution, it ensures that it receives light from all directions and realizes gas emission. The carbon dioxide replenishment valve is a valve component that regulates the amount of carbon dioxide input in the spirulina cultivation system, and can increase or decrease the carbon dioxide supply as needed.

[0091] S5. After deploying the transparent tank, the carbon dioxide sensor, the oxygen sensor, the edge computing module, the growth control module, and the instruction generation function, the photobiophotoreactor is prepared.

[0092] In one embodiment of the present invention, the preparation of the photobiophotoreactor includes: assembling a transparent tank, a carbon dioxide sensor, an oxygen sensor, an edge computing module, a growth control module, and an instruction generation function into a dedicated testing fixture, and introducing standard air into the transparent tank; checking whether the concentration deviation between the carbon dioxide sensor and the oxygen sensor is within the standard range under the condition of introducing standard air; and performing a water pressure holding test on the transparent tank when the concentration deviation between the carbon dioxide sensor and the oxygen sensor is within the standard range, so that the photobiophotoreactor is prepared after confirming that there is no leakage in the transparent tank.

[0093] Optionally, the transparent tank, all sensors, modules, and command generation functions are assembled into a dedicated testing fixture, and standard air is introduced into the transparent tank. The dedicated testing fixture is a customized fixed support and pipeline connection fixture, which can achieve stable assembly of each component and connection of the gas passage. Introducing standard air into the tank relies on conventional gas delivery and pipeline control technology. The introduced air is atmospheric pressure clean standard air that meets industrial standards, with known conventional concentrations of carbon dioxide and oxygen. Furthermore, under the scenario of introducing standard air, the concentration deviation between the carbon dioxide sensor and the oxygen sensor is checked to see if it is within the standard range. The real-time detection values ​​of the sensor are compared with the standard concentrations of carbon dioxide and oxygen in the air to calculate the deviation between the actual detection values ​​and the standard values, thereby verifying the accuracy of the sensor in measuring gas concentration. Furthermore, a water pressure holding test is performed on the transparent tank: clean water is filled into the transparent tank through a dedicated water inlet and pressurized to the pressure holding pressure specified in the equipment design. After closing all passages, the pressure holding time is maintained for the set duration. The water pressure holding test is a routine method for testing the sealing performance of closed containers such as photobioreactor tanks, and is used to determine whether there are any sealing defects. Finally, when there are no sealing defects, the preparation of the photobioreactor is completed.

[0094] Reference Figure 4 The image shown is an explosive assembly schematic diagram of a photobioreactor prepared according to an embodiment of the present invention. Figure 4 In the middle, from the top aluminum alloy flip-top cover and dustproof mesh, to the cylinder block bracket and light fixture connection bracket in the middle, and the LED lights, then to the core transparent cylinder block and its supporting sensor components and air passages, below are the graphene heating film and foam insulation board, and at the bottom is a base integrating functional modules such as an air pump and a liquid storage tank. Note that the "bottom" here specifically refers to the subsequent components. Figure 5 Photobioreactor in Figure 4 Not shown in the middle, the right side separately displays the assembly structure of the cabinet with control screen and front panel. The aluminum alloy flip-top cover and dustproof net are responsible for the protection and dust prevention of the top of the equipment. The cylinder bracket supports the transparent cylinder and fixes the LED lights to provide customized lighting. The sensor components monitor the gas concentration in the cylinder in real time. The air channel, together with the air pump, ensures the flow of algae liquid and gas exchange. The graphene heating film and insulation board realize temperature control. The functional modules integrated in the base and the control screen on the right constitute the core of the intelligent control of the equipment.

[0095] Reference Figure 5 The image shown is a schematic diagram of a photobioreactor prepared according to an embodiment of the present invention. Figure 5In the diagram, 1 represents the base assembly, 2 represents the container (glassware), 3 represents the dustproof net, 4 represents the top cover assembly, 5 represents the controller display screen, 6 represents the sensor assembly, 9 represents the LED light source assembly, 10 represents the carbon dioxide source, 11 represents the air pump, 12 represents the graphene heating film, 13 represents the heat preservation film, 14 represents the automatic algae collection device, 15 represents the automatic drainage device, 16 represents the automatic water filling device, 17 represents the PC rounded corner frame, and 18 represents algae solution + nutrient solution + algae seed.

[0096] Reference Figure 6 The image shown is a multi-view engineering schematic diagram of a photobioreactor prepared according to an embodiment of the present invention. Figure 6 The diagram includes a top view, a main sectional view, a side view, and a rear view. The top view shows the flat appearance of the top of the equipment and the layout of the small integrated control module on the right. The main sectional view in the middle shows the internal functional areas of the equipment. The left side contains auxiliary modules such as liquid storage tanks, the middle part is the core culture tank and the supporting gas circulation pipeline, and the right side is the operation panel that integrates control buttons and interfaces, which intuitively shows the arrangement of the internal functional components of the equipment. The side view on the right shows the depth structure of the equipment and the vertical stacking relationship of the components. The rear view on the side shows the simple and closed appearance of the back of the equipment.

[0097] like Figure 7 The diagram shown is a functional block diagram of a photobioreactor preparation system according to the present invention.

[0098] The photobiophotoreactor preparation system 700 of this invention can be installed in an electronic device. Depending on the functions it performs, the photobiophotoreactor preparation system includes a concentration acquisition module 701, a carboxyl-oxygen ratio calculation module 702, a function configuration module 703, a growth control module 704, and a reactor preparation module 705. The module described in this invention can also be referred to as a unit, which refers to a series of computer program segments that can be executed by the processor of an electronic device and can perform a fixed function, and are stored in the memory of the electronic device.

[0099] In this embodiment of the invention, the functions of each module / unit are as follows:

[0100] The concentration acquisition module 701 is used to install a carbon dioxide sensor and an oxygen sensor in the transparent tank, so as to acquire the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank through the carbon dioxide sensor and the oxygen sensor.

[0101] The carboxyl-oxygen ratio calculation module 702 is used to connect the edge computing module to the carbon dioxide sensor and the oxygen sensor respectively, so as to calculate the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank through the carbon dioxide concentration difference and the oxygen concentration difference.

[0102] The function configuration module 703 is used to connect the edge computing module to the growth control module in the transparent tank, so as to configure the edge computing module to generate an instruction that converts the preset carboxylase carboxyl-oxygen ratio into a regulation instruction of the growth control module.

[0103] The growth control module 704 is used in which the growth control module includes a light module, a temperature control component, a water flow drive component, and a carbon dioxide replenishment valve;

[0104] The reactor preparation module 705 is used to prepare a photobiophotoreactor after deploying the transparent tank, the carbon dioxide sensor, the oxygen sensor, the edge computing module, the growth control module, and the instruction generation function.

[0105] In detail, the modules in the photobiophotoreactor preparation system 700 described in this embodiment of the invention employ the same methods as described above during use. Figure 1 The preparation method of the photobiophotoreactor described herein uses the same technical means and can produce the same technical effect, so it will not be repeated here.

[0106] In one embodiment, a computer device is provided, which may be a server or a client, and its internal structure diagram may be as follows: Figure 8 As shown, the computer device includes a processor, memory, network interface, and database connected via a system bus. The processor provides computational and control capabilities. The memory includes non-volatile and / or volatile storage media and internal memory. The non-volatile storage media stores the operating system, computer programs, and database. The internal memory provides an environment for the operation of the operating system and computer programs in the non-volatile storage media. The network interface is used for communication with external clients via a network connection. When the computer program is executed by the processor, it implements the functions or steps of a method for preparing a photobioreactor, either on the server or client side.

[0107] In one embodiment, a computer device is provided, including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the computer program to perform the following steps:

[0108] A carbon dioxide sensor and an oxygen sensor are installed in a transparent tank to collect the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank.

[0109] The edge computing module is connected to the carbon dioxide sensor and the oxygen sensor respectively, so as to calculate the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank by the difference between the carbon dioxide concentration and the difference between the oxygen concentration;

[0110] The edge computing module is connected to the growth control module in the transparent tank to configure the edge computing module to generate instructions that convert the preset carboxylase carboxyl-oxygen ratio into the regulation instructions of the growth control module.

[0111] The growth control module includes a light module, a temperature control component, a water flow drive component, and a carbon dioxide replenishment valve.

[0112] After deploying the transparent tank, the carbon dioxide sensor, the oxygen sensor, the edge computing module, the growth control module, and the instruction generation function, the photobiophotoreactor is prepared.

[0113] In one embodiment, a computer-readable storage medium is provided having a computer program stored thereon, the computer program performing the following steps when executed by a processor:

[0114] A carbon dioxide sensor and an oxygen sensor are installed in a transparent tank to collect the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank.

[0115] The edge computing module is connected to the carbon dioxide sensor and the oxygen sensor respectively, so as to calculate the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank by the difference between the carbon dioxide concentration and the difference between the oxygen concentration;

[0116] The edge computing module is connected to the growth control module in the transparent tank to configure the edge computing module to generate instructions that convert the preset carboxylase carboxyl-oxygen ratio into the regulation instructions of the growth control module.

[0117] The growth control module includes a light module, a temperature control component, a water flow drive component, and a carbon dioxide replenishment valve.

[0118] After deploying the transparent tank, the carbon dioxide sensor, the oxygen sensor, the edge computing module, the growth control module, and the instruction generation function, the photobiophotoreactor is prepared.

[0119] It should be noted that the functions or steps that can be implemented by the computer-readable storage medium or computer device described above can be referred to the relevant descriptions on the server side and client side in the foregoing method embodiments. To avoid repetition, they will not be described one by one here.

[0120] Those skilled in the art will understand that all or part of the processes in the methods of the above embodiments can be implemented by a computer program instructing related hardware. The computer program can be stored in a non-volatile computer-readable storage medium. When executed, the computer program can include the processes of the embodiments of the above methods. Any references to memory, storage, databases, or other media used in the embodiments provided in this application can include non-volatile and / or volatile memory. Non-volatile memory may include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in a variety of forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), dual data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link DRAM (SLDRAM), RAMbus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), etc.

[0121] Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the above-described division of functional units and modules is used as an example. In practical applications, the above functions can be assigned to different functional units and modules as needed, that is, the internal structure of the device can be divided into different functional units or modules to complete all or part of the functions described above.

[0122] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0123] Finally, it should be noted that in the above embodiments, each embodiment can be combined with each other or independent. Deleting any one of them will not affect the technical implementation of other embodiments. The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a photobiophotoreactor, characterized in that, The method includes: A carbon dioxide sensor and an oxygen sensor are installed in a transparent tank to collect the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank. The edge computing module is connected to the carbon dioxide sensor and the oxygen sensor respectively, so as to calculate the preset carboxylase carboxyl-oxygen ratio of spirulina in the transparent tank by the difference between the carbon dioxide concentration and the difference between the oxygen concentration; The edge computing module is connected to the growth control module in the transparent tank to configure the edge computing module to generate instructions that convert the preset carboxylase carboxyl-oxygen ratio into the regulation instructions of the growth control module. The growth control module includes a light module, a temperature control component, a water flow drive component, and a carbon dioxide replenishment valve. After deploying the transparent tank, the carbon dioxide sensor, the oxygen sensor, the edge computing module, the growth control module, and the instruction generation function, the photobiophotoreactor is prepared.

2. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, The preset carboxyl-oxygen ratio of spirulina in the transparent tank is calculated by using the difference in carbon dioxide concentration and the difference in oxygen concentration, including: Query the basal respiratory rate of the spirulina; The preset carboxyl-oxygen ratio of the spirulina is calculated based on the carbon dioxide concentration difference, the oxygen concentration difference, and the basal respiration rate.

3. The method for preparing a photobiophotoreactor as described in claim 2, characterized in that, The basal respiratory rate of the spirulina was queried, including: The basic respiration rate of Spirulina under standard culture conditions is pre-stored in the edge computing module; The basic respiration rate of the spirulina is queried in the edge computing module.

4. The method for preparing a photobiophotoreactor as described in claim 2, characterized in that, Based on the carbon dioxide concentration difference, the oxygen concentration difference, and the basal respiration rate, the preset carboxylase carboxyl-oxygen ratio of the spirulina is calculated, including: The preset carboxyl-oxygen ratio of the spirulina was calculated using the following formula: in, This indicates the preset carboxyl-oxygen ratio of the carboxylase. This represents the difference in carbon dioxide concentration. This represents the difference in oxygen concentration. This indicates the basal respiratory rate.

5. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, Connecting the edge computing module to the carbon dioxide sensor and the oxygen sensor respectively includes: The output lines of the carbon dioxide sensor and oxygen sensor are crimped to the analog input terminals of the edge computing module to complete the physical connection.

6. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, Carbon dioxide and oxygen sensors are installed inside a transparent tank, including: Through holes are made in the tank wall to insert carbon dioxide sensors and oxygen sensors respectively.

7. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, The transparent tank is insulated with graphene and foam.

8. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, The carbon dioxide sensor and the oxygen sensor collect the difference in carbon dioxide concentration and the difference in oxygen concentration in the transparent tank, including: The carbon dioxide sensor simultaneously collects the concentrations of carbon dioxide in the top gas phase and the bottom liquid phase. The carbon dioxide concentration difference is obtained by subtracting the carbon dioxide concentration in the bottom liquid phase from the top gas phase concentration. The oxygen sensor simultaneously collects the oxygen concentration in the top gas phase and the oxygen concentration in the bottom liquid phase. The oxygen concentration difference is obtained by subtracting the oxygen concentration in the liquid phase from the oxygen concentration in the top gas phase.

9. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, Connecting the edge computing module to the growth control module in the transparent tank includes: Connect the output terminals of the edge computing module to the corresponding control ports of the illumination module, temperature control component, water flow drive component, and carbon dioxide replenishment valve using shielded cables, so as to connect the edge computing module to the growth control module in the transparent tank.

10. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, The edge computing module is configured to generate an instruction function that converts the preset carboxylase carboxyl-oxygen ratio into a regulation instruction for the growth control module, including: The edge computing module incorporates a preset optimal range of carboxyl-oxygen ratio for spirulina growth. Configure the threshold comparison logic of the edge computing module, wherein the threshold comparison logic includes: When the preset carboxylase carboxyl-oxygen ratio is lower than the preset optimal range of carboxylase carboxyl-oxygen ratio, a regulatory instruction to inhibit photorespiration and promote carboxylation reaction is generated; when the preset carboxylase carboxyl-oxygen ratio is higher than the preset optimal range of carboxylase carboxyl-oxygen ratio, a regulatory instruction to optimize carbon source supply and improve oxygen release efficiency is generated. Establish an instruction output protocol for the edge computing module so that the generated control instructions match the communication interface of the growth control module, and then complete the configuration of the instruction generation function.

11. The method for preparing a photobiophotoreactor as described in claim 1, characterized in that, The fabrication of photobiophotoreactors includes: The transparent tank, carbon dioxide sensor, oxygen sensor, edge computing module, growth control module and instruction generation function are assembled into a special test fixture to introduce standard air into the transparent tank. Under standard air conditions, check whether the concentration deviation between the carbon dioxide sensor and the oxygen sensor is within the standard range; When the concentration deviation between the carbon dioxide sensor and the oxygen sensor is within the standard range, a water pressure holding test is performed on the transparent tank to confirm that there is no leakage in the transparent tank before the photobiophotoreactor is prepared.

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