Heart-spleen coordination regulating composition based on chinese yam composite peptide, preparation method and application thereof in improving heart-spleen dysfunction

Abstract

This invention relates to the field of biomedical technology, specifically to a heart-spleen synergistic regulatory composition based on Chinese yam complex peptides, its preparation method, and its application in improving heart-spleen dysfunction. The method involves first gelatinizing Chinese yam, then sequentially performing primary endopeptidation with papain and secondary fine exopeptidation with flavor protease, followed by separation via a 1000 Da ultrafiltration membrane to directionally prepare a Chinese yam complex peptide with a peptide fraction of at least 90% having a molecular weight less than 1000 Da. This complex peptide is then combined with extracts of ginseng, lotus seed, longan pulp, and tangerine peel in a specific ratio. The yam peptides obtained by this invention can self-assemble with macromolecular hydrophobic components such as ginsenosides in the system to form microscopic peptide-saponin complexes, acting as biological delivery carriers and significantly improving the transmembrane absorption rate of macromolecular active ingredients in individuals with weak spleen and stomach. Simultaneously, the two-step enzymatic hydrolysis process achieves the simultaneous retention of highly active small molecules and precise debittering.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and more specifically, to a heart-spleen synergistic regulatory composition based on Chinese yam complex peptides, its preparation method, and its application in improving heart-spleen dysfunction. Background Technology

[0002] Traditional Chinese medicine theory holds that the heart governs blood, and the spleen controls blood; the heart governs the mind, and the spleen governs digestion and transformation. Prolonged mental stress, dietary imbalances, or recovery from a serious illness can easily lead to deficiency of both the heart and spleen, or dysfunction of both, clinically manifesting as palpitations, insomnia, poor appetite, loose stools, and fatigue. For these symptoms, traditional Chinese medicine often uses classic formulas such as "Gui Pi Tang" with modifications, frequently employing medicinal and edible herbs such as ginseng, yam, longan (dried longan pulp), and dried tangerine peel for conditioning.

[0003] However, existing products or solutions for treating heart and spleen imbalances have the following significant technical shortcomings: First, traditional compound prescriptions often involve decocting or extracting the original medicinal materials with water or alcohol. The active ingredients have large molecular weights, making them difficult for those with weak spleen and stomach to absorb, easily leading to adverse reactions due to insufficient nourishment. For example, patent publication number CN116350725B discloses a traditional Chinese medicine composition for treating insomnia due to deficiency of both heart and spleen, containing more than ten kinds of traditional Chinese medicine, including ginseng, yam, and jujube seed. Although this existing technology follows the traditional Chinese medicine logic of tonifying the heart and spleen, its preparation process still mainly relies on conventional decoction and concentration. Because the extract is enriched with large molecules such as polysaccharides, crude proteins, and crude saponins, it is not only difficult for patients with heart and spleen disorders whose spleen and stomach functions are already extremely weak, but it may even increase the burden on the gastrointestinal tract, resulting in extremely low bioavailability of core active ingredients such as ginsenosides, making it difficult to exert the intended targeted conditioning effect.

[0004] Secondly, existing yam extraction or peptide preparation processes are simplistic and lack precise nutritional intervention designs targeting the heart and spleen synergy and absorption-enhancing carriers. To improve yam absorption, some existing technologies have attempted to prepare yam peptides using enzymatic methods. For example, patent publication number CN101348819B discloses a method for extracting yam protein complexes using cellulase, hemicellulase, and protease. However, this type of patent technology mainly focuses on the crude extraction of yam crude protein or large-molecule peptides for basic nutritional supplementation; its enzymatic hydrolysis process does not target the selective retention of small-molecule active peptides (e.g., <1000Da). More importantly, existing technologies generally treat yam peptides as a single nutrient, failing to recognize and utilize the enormous potential of small-molecule yam peptides as biomolecule carriers to promote the transmembrane absorption of other poorly soluble active ingredients (such as ginsenosides).

[0005] In addition, traditional plant enzymatic hydrolysis processes often involve severe exposure of hydrophobic amino acids, resulting in products with a strong bitter taste, which severely limits their application in special medical foods and oral nutritional solutions. Summary of the Invention

[0006] The purpose of this invention is to provide a cardiospleen-spleen synergistic regulatory composition based on Chinese yam complex peptides, its preparation method, and its application in improving cardiospleen dysfunction, so as to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides a method for preparing a cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides, comprising the following steps: S1. Material pretreatment and gelatinization: Take Chinese yam, wash, peel, dry and crush it, add purified water, mix at a material-to-liquid mass-to-volume ratio of 1:10 to 1:15 (g / mL), gelatinize at 85 to 95℃ for 30 to 40 minutes to obtain yam gelatinized liquid. S2. Primary enzymatic hydrolysis: Adjust the temperature of the yam gelatinization solution to 55-60℃ and the pH to 6.5-7.0. Add papain, the amount of which is 0.3%-0.5% of the dry weight of the substrate. Incubate for 1.5-2.5 hours for enzymatic hydrolysis, and then heat to 90℃ for 10 minutes to inactivate the enzyme to obtain the primary enzymatic hydrolysate. S3, Secondary precise enzymatic hydrolysis: Cool the primary enzymatic hydrolysate to 50-55℃, adjust the pH to 6.0-6.5, add flavor protease, the amount of flavor protease added is 0.1%-0.2% of the dry weight of the substrate, continue to incubate for 2-3 hours, then heat to 90℃ to inactivate the enzyme for 10 minutes to obtain the two-step enzymatic hydrolysate; S4. Retention and Purification: The two-step enzymatic hydrolysate is separated by ultrafiltration through an ultrafiltration membrane with a molecular weight cutoff of 1000 Da. The dialysate is collected, concentrated, and dried to obtain Chinese yam compound peptide powder, in which the mass proportion of peptides with a molecular weight <1000 Da is not less than 90%. S5. Composition Compatibility: Mix the Chinese yam complex peptide with ginseng extract, lotus seed extract, longan pulp extract and tangerine peel extract in a set ratio until homogeneous.

[0008] Furthermore, the present invention also provides a cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides prepared by the above preparation method, wherein the effective components, by weight, include: 30-50 parts of Chinese yam complex peptide, 5-15 parts of ginseng extract, 10-20 parts of lotus seed extract, 10-20 parts of longan pulp extract, and 5-10 parts of tangerine peel extract.

[0009] As a preferred embodiment of the present invention, the Chinese yam complex peptide can self-assemble with ginsenosides in ginseng extract in an aqueous system to form a peptide-saponin complex.

[0010] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention relates to a heart-spleen synergistic regulatory composition based on Chinese yam complex peptides, its preparation method, and its application in improving heart-spleen dysfunction. In a compound system, the Chinese yam complex peptides with a molecular weight <1000 Da, prepared by the dual enzymatic hydrolysis and ultrafiltration process of this invention, not only possess spleen-strengthening effects but also act as a carrier for biomolecules. This specific segment of the small molecule peptide can form nanoscale complexes with highly hydrophobic, large-molecule ginsenosides in ginseng extract through intermolecular forces, significantly increasing the apparent solubility of ginsenosides and achieving active targeted transport via the peptide transporter protein (PepT1) of intestinal epithelial cells. This effectively solves the problem of low conversion rate of macromolecular pharmacological components in individuals with weak spleen and stomach.

[0011] Secondly, traditional plant polypeptide enzymatic hydrolysis processes often produce a severe bitter taste due to the exposure of a large number of hydrophobic amino acid residues. This invention addresses this by controlling a dual-enzymatic hydrolysis process. In the first stage, papain is used for endoplasty to increase polypeptide yield. In the second stage, a flavor protease is introduced under controlled temperature and pH conditions. The exonuclease system not only further cleaves the polypeptide into an easily absorbed window below 1000 Da, but also targets and removes the bitter, hydrophobic amino acid residues at the peptide chain ends. This improves the sensory quality of the product without the need for additional flavoring agents, facilitating its industrial application in medical foods and solid beverages.

[0012] Furthermore, based on the efficient delivery characteristics of the Chinese yam complex peptide in this application, the retention time of tonifying ingredients such as ginseng and longan pulp in the gastrointestinal tract is significantly shortened; at the same time, the tangerine peel extract in the formula can further exert the effect of regulating qi. The two work together to effectively avoid the side effects of greasy and stomach-damaging caused by the accumulation of macromolecular substances in the local microenvironment in traditional tonifying formulas. Attached Figure Description

[0013] Figure 1 This is an overall flowchart of Embodiment 1 of the present invention; Figure 2 This is a schematic diagram of the microscopic self-assembly and transmembrane absorption mechanism of the composition in Experimental Example 3 of the present invention.

[0014] The meanings of the labels in the diagram are as follows: 10. Chinese yam complex peptide; 20. Ginsenosides; 30. Peptide-saponin complex; 40. Intestinal epithelial cells; 41. Brush border; 50. Peptide transporter protein; 60. Blood circulation. Detailed Implementation

[0015] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0016] Example 1: This embodiment of the invention provides a method for preparing a heart-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides, comprising the following steps: S1. Material pretreatment and gelatinization: Weigh 1000g of Chinese yam slices, wash and dry them, then pulverize them through an 80-mesh sieve. Add purified water and mix them at a mass-to-volume ratio of 1:12 (g / mL). Gelatinize at 90℃ for 35 minutes to obtain yam gelatinized liquid.

[0017] S2. Primary enzymatic hydrolysis: Adjust the temperature of the yam gelatinization solution to 58℃, adjust the pH to 6.8 using a 1mol / L NaOH or HCl solution, add papain (enzyme activity ≥800,000 U / g) at a rate of 0.4% of the substrate dry weight, incubate in a constant temperature water bath shaker for 2 hours, then rapidly raise the temperature to 90℃ and maintain it for 10 minutes to inactivate the enzyme, cool, and obtain the primary enzymatic hydrolysate.

[0018] S3, Secondary Precision Enzymatic Hydrolysis: Cool the primary enzymatic hydrolysate to 52℃, adjust the pH to 6.2, add flavor protease (enzyme activity ≥ 30,000 U / g) at a rate of 0.15% of the substrate dry weight, and continue enzymatic hydrolysis for 2.5 hours. Then, heat to 90℃ to inactivate the enzyme for 10 minutes, cool, and centrifuge to remove residue to obtain the two-step enzymatic hydrolysate.

[0019] S4. Retention and purification: The above two-step enzymatic hydrolysate is pumped into an ultrafiltration membrane module with a molecular weight cutoff of 1000 Da for ultrafiltration separation. The ultrafiltration permeate is collected, concentrated under reduced pressure, and then spray-dried (inlet air temperature 160℃, outlet air temperature 85℃) to obtain Chinese yam complex peptide 10.

[0020] S5. Composition Compatibility: Weigh 10,400g of the above-mentioned Chinese yam complex peptide, 100g of ginseng extract (total ginsenoside content ≥80%), 150g of lotus seed extract, 150g of longan pulp extract, and 80g of tangerine peel extract, and mix them evenly in a three-dimensional mixer to obtain the final product.

[0021] Example 2: This embodiment of the invention provides a method for preparing a cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides, comprising the following steps: S1. Material pretreatment and gelatinization: Weigh 1000g of Chinese yam slices, crush them through an 80-mesh sieve, add purified water, and gelatinize at 85℃ for 30 minutes with a material-to-liquid mass-to-volume ratio of 1:10 (g / mL).

[0022] S2. Primary enzymatic hydrolysis: Adjust the temperature to 55℃, adjust the pH to 6.5, add 0.3% of the substrate dry weight of papain, incubate for 1.5 hours, and then inactivate the enzyme at 90℃ for 10 minutes.

[0023] S3, Secondary Precision Enzymatic Hydrolysis: Cool to 50℃, adjust pH to 6.0, add 0.1% of the substrate dry weight of flavor protease, incubate for 2 hours, then inactivate the enzyme at 90℃ for 10 minutes, and centrifuge to remove residue.

[0024] S4. Retention and purification: After separation by a 1000 Da ultrafiltration membrane, the permeate was concentrated and spray-dried to obtain Chinese yam complex peptide 10.

[0025] S5. Composition Compatibility: Weigh 10300g of the above-mentioned Chinese yam compound peptide, 50g of ginseng extract, 100g of lotus seed extract, 100g of longan pulp extract, and 50g of tangerine peel extract, and mix them evenly to obtain the final product.

[0026] Example 3: This embodiment of the invention provides a method for preparing a cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides, comprising the following steps: S1. Material pretreatment and gelatinization: Weigh 1000g of Chinese yam slices, crush them through an 80-mesh sieve, add purified water, and gelatinize at 95℃ for 40 minutes with a material-to-liquid mass-to-volume ratio of 1:15 (g / mL).

[0027] S2, Primary enzymatic hydrolysis: Adjust the temperature to 60℃, adjust the pH to 7.0, add 0.5% of the substrate dry weight of papain, incubate for 2.5 hours, and then inactivate the enzyme at 90℃ for 10 minutes.

[0028] S3, Secondary Precision Enzymatic Hydrolysis: Cool to 55℃, adjust pH to 6.5, add 0.2% of the substrate dry weight of flavor protease, incubate for 3 hours, then inactivate the enzyme at 90℃ for 10 minutes, and centrifuge to remove residue.

[0029] S4. Retention and purification: After separation by a 1000 Da ultrafiltration membrane, the permeate was concentrated and spray-dried to obtain Chinese yam complex peptide 10.

[0030] S5. Composition Compatibility: Weigh 10500g of the above-mentioned Chinese yam compound peptide, 150g of ginseng extract, 200g of lotus seed extract, 200g of longan pulp extract, and 100g of tangerine peel extract, and mix them evenly to obtain the final product.

[0031] Comparative Example 1: Single-enzyme digestion comparison process, lacking exonuclease modification.

[0032] Preparation steps S1, S2, S4, and S5 are the same as in Example 1. The secondary precise enzymatic hydrolysis process in S3 is omitted; that is, after primary enzymatic hydrolysis and enzyme inactivation, ultrafiltration and drying are performed directly to obtain the composition.

[0033] Comparative Example 2: Traditional water extraction process.

[0034] Preparation steps: Weigh 1000g of Chinese yam, add 12 times the amount of water, soak for 1 hour, decoct for 1.5 hours, and filter; add 10 times the amount of water to the dregs, decoct for 1 hour, filter, combine the two filtrates, concentrate and spray dry to obtain Chinese yam extract powder (without enzymatic hydrolysis and ultrafiltration stage), take 400g of the powder and mix evenly with an equal amount of ginseng, lotus seed, longan pulp and tangerine peel extract in Example 1 to obtain the composition.

[0035] Comparative Example 3: Non-specific enzyme system replacement process.

[0036] The preparation steps are the same as in Example 1, except that the flavor protease in the secondary precise enzymatic hydrolysis of S3 is replaced with an equal amount of conventional industrial alkaline protease, and the enzymatic hydrolysis is carried out under the optimal conditions of alkaline protease (pH 8.0, temperature 55°C). The remaining steps are completely consistent with the formulation.

[0037] Experimental Example 1: Molecular weight distribution and sensory and physicochemical index evaluation of extracts.

[0038] The molecular weight distribution of the prepared Chinese yam powders was determined using a high-performance liquid chromatography (HPLC) instrument equipped with a TSK gel G2000 SWXL gel chromatography column. Simultaneously, ten professionally trained sensory evaluators scored the bitterness of a 1% aqueous solution of each powder (0 points represented no bitterness, 10 points represented extreme bitterness, and the average value was taken). The test results are shown in Table 1.

[0039] Table 1: Molecular weight distribution and bitterness score results of each example and comparative example

[0040] As shown in Table 1, Examples 1-3 of this invention consistently achieved the goal of having a small molecule peptide ratio of <1000Da greater than 90% and extremely low bitterness. Comparative Example 2 (traditional water extraction) mainly consisted of large-molecule polysaccharides and proteins, and the molecular weight was not effectively degraded. Comparative Example 1 used only papain (an endopeptide), which, although it cleaved long-chain proteins, resulted in the exposure of a large amount of bitter, hydrophobic amino acids, producing an extremely strong bitter taste, and the degradation depth was insufficient. Comparative Example 3 replaced it with an alkaline protease, but due to the lack of an exopeptide system, not only did the small molecule peptide retention rate not reach the level of this invention, but it also had a strong metallic bitter taste. This application introduces a flavor protease and controls it within a specific temperature range and pH. Its rich exopeptide system successfully targeted and cleaved the terminal bitter amino acids, while simultaneously finely trimming the peptide chain to the optimal absorption window.

[0041] Experimental Example 2: Verification of the solubilizing properties of microscopic self-assembled systems Take 10g of each of the compositions prepared in Examples 1-3 and Comparative Example 2, disperse them in 100mL of simulated gastric fluid at 37℃, stir magnetically for 30min, and then let stand. Measure the transmittance of the supernatant at 600nm using a UV spectrophotometer, and observe and record the sedimentation at the bottom. The test results are shown in Table 2.

[0042] Table 2: Solubility characteristics of each composition in simulated gastric fluid

[0043] As shown in Table 2, in Comparative Example 2 (traditional Chinese medicine formulation), the core macromolecular components, such as ginsenoside 20, have extremely strong hydrophobic cores, which easily aggregate and form insoluble precipitates in the gastric juice environment, resulting in a transmittance of only 45.2%. In contrast, the transmittance of Examples 1-3 of this invention is all above 90%, achieving clarification and solubilization of the system. The small molecule peptides of Dioscorea opposita prepared in this invention are rich in amphiphilic fragments, which can self-assemble with hydrophobic macromolecules such as ginsenoside 20 in an aqueous system through hydrogen bonding and hydrophobic interactions to form stable nanoscale peptide-saponin micelle complexes. This carrier structure effectively masks the hydrophobicity of the active ingredients, significantly improving the apparent solubility of the macromolecular active substances.

[0044] Experimental Example 3: Caco-2 Small Intestinal Epithelial Cell Transmembrane Transport Assay A human colon cancer intestinal wall cell (Caco-2) monoclonal cell in vitro absorption model was constructed. Cells were cultured in Transwell chambers, and after the transmembrane resistance stabilized, equal concentrations of ginseng total saponin standard solution (blank control group) and solutions containing ginseng saponin 20 (example and comparative solutions) were added to the top side (AP side). After 2 hours of incubation, the solution from the basal side (BL side) was collected, and the apparent permeability coefficient (Papp) of the core ginseng component (calculated as ginseng saponin Rg1) was determined by HPLC-MS. The test results are shown in Table 3.

[0045] Table 3: Apparent permeability coefficient (Papp) of ginsenoside Rg1 in each group

[0046] As shown in Table 3, the transmembrane transport data at the cellular level indicate that ginsenoside 20 alone (blank control) and the traditional water-extracted compound (comparative example 2) are limited by their large molecular weight and poor permeability, and cannot effectively penetrate the small intestinal epithelial cell barrier; their Papp values ​​are both below 2.0 × 10⁻⁶. -6 cm / s, which is considered a poorly absorbed substance. However, the Papp values ​​in Examples 1-3 of this invention all showed an order-of-magnitude increase. Combined with... Figure 2As shown, the Chinese yam complex peptide 10 prepared in this invention acts as a delivery carrier and self-assembles with ginsenoside 20 to form a stable peptide-saponin complex 30. When it comes into contact with the brush border 41 of intestinal epithelial cells 40, the peptide transport protein 50 carries the internal saponin components into the bloodstream through an active transport system and enters the blood circulation 60. This solves the technical defect of traditional Chinese medicine heart and spleen conditioning formulas in people with weak spleen and stomach who cannot tolerate tonics, and achieves targeted and efficient heart and spleen synergistic intervention.

[0047] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a heart and spleen coordinating composition based on a complex peptide of Chinese yam, characterized in that, Includes the following steps: S1. Material pretreatment and gelatinization: Take Chinese yam, wash, peel, dry and crush it, add purified water, mix at a material-to-liquid mass-to-volume ratio of 1:10 to 1:15 (g / mL), gelatinize at 85 to 95℃ for 30 to 40 minutes to obtain yam gelatinized liquid. S2. Primary enzymatic hydrolysis: Adjust the temperature of the yam gelatinization solution to 55-60℃ and the pH to 6.5-7.

0. Add papain, the amount of which is 0.3%-0.5% of the dry weight of the substrate. Incubate for 1.5-2.5 hours for enzymatic hydrolysis, and then heat to 90℃ for 10 minutes to inactivate the enzyme to obtain the primary enzymatic hydrolysate. S3, Secondary precise enzymatic hydrolysis: Cool the primary enzymatic hydrolysate to 50-55℃, adjust the pH to 6.0-6.5, add flavor protease, the amount of flavor protease added is 0.1%-0.2% of the dry weight of the substrate, continue to incubate for 2-3 hours, then heat to 90℃ to inactivate the enzyme for 10 minutes to obtain the two-step enzymatic hydrolysate; S4. Retention and purification: The two-step enzymatic hydrolysate was separated by ultrafiltration through an ultrafiltration membrane with a molecular weight cutoff of 1000 Da. The dialysate was collected, concentrated, and dried to obtain the Chinese yam complex peptide (10), wherein the peptides with a molecular weight <1000 Da accounted for no less than 90% of the total mass. S5. Composition Compatibility: Mix the Chinese yam complex peptide (10) with ginseng extract, lotus seed extract, longan pulp extract and tangerine peel extract in proportion to obtain the final product.

2. The preparation method of the cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 1, characterized in that, In step S1, the mixture is prepared at a mass-to-volume ratio of 1:12 (g / mL) and gelatinized at a constant temperature of 90°C for 35 minutes.

3. The method for preparing the cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 1, characterized in that, In step S2, the temperature of the yam gelatinization solution is adjusted to 58°C, the pH is adjusted to 6.8, and the amount of papain added is 0.4% of the dry weight of the substrate. The mixture is then incubated for 2 hours for enzymatic hydrolysis.

4. The preparation method of the cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 1, characterized in that, In step S3, the primary enzymatic hydrolysate is cooled to 52°C, the pH is adjusted to 6.2, and the amount of flavor protease added is 0.15% of the dry weight of the substrate. The enzymatic hydrolysis is then continued for 2.5 hours.

5. A heart-spleen synergistic regulatory composition based on Chinese yam complex peptides, prepared by the method of preparing the heart-spleen synergistic regulatory composition based on Chinese yam complex peptides according to any one of claims 1-4.

6. The cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 5, characterized in that, By weight, it includes: 30-50 parts of Chinese yam compound peptide (10), 5-15 parts of ginseng extract, 10-20 parts of lotus seed extract, 10-20 parts of longan pulp extract, and 5-10 parts of tangerine peel extract.

7. The cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 6, characterized in that, By weight, it includes: 40 parts of Chinese yam compound peptide (10), 10 parts of ginseng extract, 15 parts of lotus seed extract, 15 parts of longan pulp extract, and 8 parts of tangerine peel extract.

8. The cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 5, characterized in that, The Chinese yam complex peptide (10) self-assembles with ginsenosides (20) in ginseng extract in an aqueous system to form a peptide-saponin complex (30).

9. The use of the cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 5 in the preparation of products for improving cardiospleen dysfunction.

10. The application of the cardiospleen-spleen synergistic regulatory composition based on Dioscorea opposita complex peptides according to claim 9 in the preparation of products for improving cardiospleen dysfunction, characterized in that, The products include special medical foods, health foods, or solid beverages.

Images