Human body temperature responsive microcapsules, methods of making and using the same

Abstract

This invention relates to the field of colloid and interface technology, specifically to a human body temperature-responsive microcapsule, its preparation method, and its applications. The method uses food-grade polyglycerol ricinoleate (PGPR) as a single emulsifying stabilizer and leverages the solid-liquid phase transition property of lard near human body temperature to prepare a microcapsule emulsion with a human body temperature-responsive release function for the loaded ingredient through a two-step process. This invention enables the microcapsules to release functional loaded ingredients in response to human body temperature, and has significant potential applications in food encapsulation, drug sustained release, and cosmetics.

Description

Technical Field

[0001] This invention relates to the field of colloid and interface chemistry, and in particular to a human body temperature-responsive microcapsule, its preparation method, and its application. Background Technology

[0002] Temperature-responsive microcapsules loaded with functional ingredients have significant market demand in food encapsulation, drug sustained release, and cosmetics. A double emulsion is an emulsion in which one emulsion is dispersed in another continuous phase, resulting in emulsion droplets containing even smaller droplets. Double emulsions are mainly classified into two types: water-in-oil-in-water (W1 / O / W2) and oil-in-water-in-oil (O1 / W / O2). The multiphase system and two-layer interfacial membrane characteristics of double emulsions make them valuable for drug delivery. For example, they can simultaneously transport water-soluble and lipid-soluble drugs, or drugs with a certain solubility in both phases. For drugs that are easily destroyed by the gastrointestinal environment, such as vitamins, probiotics, and coenzyme Q10, they can be encapsulated in one phase to improve drug stability and efficacy. The unique two-membrane three-phase structure of double emulsions (two oil-water interfacial layers, two aqueous phases and one oil phase, or two oil phases and one aqueous phase) is the main method for preparing temperature-responsive release microcapsules loaded with functional components.

[0003] Traditional methods for preparing dual emulsions often employ two small-molecule surfactants with different hydrophilic-lipophilic balance (HLB) values ​​as emulsifiers. This not only requires emulsifier screening and involves numerous steps, but also results in emulsions with a wide droplet size distribution and poor stability. To overcome the shortcomings of dual emulsifiers, existing technologies have been improved. For example, patent document CN120678725A discloses a method for preparing oil-in-water-in-oil (O1 / W / O2) dual emulsions using food-grade polyglycerol ricinoleate (PGPR) as a single emulsion stabilizer through a one-step shear-stirring process.

[0004] However, the inventors of this application have discovered that the above-mentioned one-step method with a single emulsifier has structural limitations when expanding to temperature-controlled release W1 / O / W2 dual emulsions. At the same time, it is extremely difficult to obtain a primary W / O emulsion with uniform, complete and stable internal aqueous phase droplets for phase change oils that solidify at room temperature. As a result, it is ultimately impossible to form W1 / O / W2 microcapsules with regular structure, high encapsulation rate and controllable release behavior. Summary of the Invention

[0005] In view of this, the purpose of this invention is to propose a human body temperature-responsive microcapsule, its preparation method and application, aiming to solve the problem in the prior art that it is difficult to prepare W1 / O / W2 type microcapsules with regular structure, high encapsulation rate and human body temperature-responsive release function by using PGPR as a single emulsifier and combining it with phase change oils that are easy to solidify at room temperature through a one-step process.

[0006] To achieve the above objectives, the present invention provides a human body temperature-responsive microcapsule, wherein the microcapsule is of type W1 / O / W2, wherein... Internal aqueous phase (W1): Contains releasable functional components; Oil phase (O): Coated outside the inner aqueous phase (W1), the oil phase (O) has solid-liquid phase transition characteristics at 25℃~40℃, which enables the microcapsules to release the functional components in the inner aqueous phase (W1) when the oil phase (O) undergoes a phase transition. External aqueous phase (W2): Coated outside the intermediate oil phase (O); The W1 / O / W2 system also includes an emulsifier for stabilizing the W1 / O interface and the O / W2 interface.

[0007] Meanwhile, a method for preparing human body temperature-responsive microcapsules is provided, comprising the following steps: S1. Mix the oil phase (O) and the internal aqueous phase (W1) at a volume ratio of 1:4~5, and then add the emulsifier; S2. The oil phase (O), the internal aqueous phase (W1), and the emulsifier are subjected to shearing and stirring for 1-30 minutes to obtain a W1 / O emulsion; S3. After adding the external aqueous phase (W2) and emulsifier to the W1 / O emulsion in sequence, the shearing and stirring operation is performed to obtain microcapsules.

[0008] Finally, an application of human body temperature-responsive microcapsules is provided, for use in food packaging, drug sustained release, or cosmetics.

[0009] The beneficial effects of this invention are as follows: This invention uses food-grade PGPR as the sole emulsifying stabilizer, utilizing the solid-liquid phase transition property of oil phases such as lard near human body temperature to physically isolate the degradation effects of external light and oxygen on the functionally loaded active ingredients inside the microcapsules, and to improve the stability of the microcapsules. A two-step process is used to prepare a microcapsule emulsion that releases functionally loaded ingredients in response to human body temperature. This invention enables microcapsules to release functionally loaded ingredients in response to human body temperature, and has enormous potential applications in food encapsulation, drug sustained release, cosmetics, and other fields.

[0010] This invention provides a two-step method for preparing human body temperature-responsive microcapsules. Through a two-step process, an emulsifier, an aqueous phase, and an oil phase are sheared and stirred to obtain a W1 / O / W2 type dual emulsion with a dispersion droplet size of 1-100 μm. This invention effectively improves upon the cumbersome process of traditional two-step methods that use two emulsifiers with different hydrophilic and lipophilic properties to separately stabilize the two oil-water interfaces within and outside the dual emulsion. It develops a method that uses only a single emulsifier to simultaneously stabilize both the inner and outer interfaces of the dual emulsion, achieving the goal of releasing the prepared microcapsules in response to human body temperature. This provides a new strategy for a dual emulsion stabilization system that is simple in process and uses inexpensive raw materials. Attached Figure Description

[0011] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only for this invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0012] Figure 1 A flowchart illustrating the preparation process of the dual emulsion microcapsules prepared according to an embodiment of the present invention; Figure 2 Digital photograph of the dual emulsion microcapsules prepared in an embodiment of the present invention; Figure 3 A fluorescence microscopy diagram of the dual emulsion microcapsules prepared according to an embodiment of the present invention; In this phase, red represents the oil phase and black represents the water phase. Figure 4 The droplet size distribution diagram of the dual emulsion microcapsules prepared in the embodiments of the present invention; Figure 5 This is a diagram showing the effect of the dual emulsion microcapsules prepared in this embodiment of the invention releasing the loaded components in response to near human body temperature; Figure 6 Optical microscope image and fluorescence microscope image of the dual emulsion microcapsule prepared in Comparative Example 1 of the present invention; In the fluorescence microscope image, red represents the oil phase and black represents the aqueous phase; Figure 7 This is an optical microscope image of the dual emulsion microcapsules prepared in Comparative Example 2 of the present invention; Figure 8 This is a fluorescence microscope image of the dual emulsion microcapsules prepared in Comparative Example 3 of the present invention; In this process, black represents the oil phase and green represents the water phase. Detailed Implementation

[0013] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments.

[0014] Existing methods for preparing dual emulsions mostly employ two small-molecule surfactants with different HLB values ​​as emulsifiers. This not only requires emulsifier screening and involves numerous steps, but also results in emulsions with wide droplet size distributions and poor stability. Furthermore, methods for preparing O1 / W / O2 dual emulsions using a single emulsifier face technical barriers in terms of structure and composition. These methods cannot be directly applied to W1 / O / W dual emulsions, or even if applied, the resulting W1 / O / W dual emulsions will ultimately fail to form well-structured microcapsules with high encapsulation efficiency and controllable release behavior.

[0015] To address the aforementioned problems, this invention provides a human body temperature-responsive microcapsule, wherein the microcapsule is of type W1 / O / W2, wherein... Internal aqueous phase (W1): Contains releasable functional components; Oil phase (O): Coated outside the inner aqueous phase (W1), the oil phase (O) has solid-liquid phase transition characteristics at 25℃~40℃, which enables the microcapsules to release the functional components in the inner aqueous phase (W1) when the oil phase (O) undergoes a phase transition. External aqueous phase (W2): Coated outside the intermediate oil phase (O); The W1 / O / W2 system also includes an emulsifier for stabilizing the W1 / O interface and the O / W2 interface.

[0016] Preferably, the emulsifier is a monoemulsifier.

[0017] Preferably, the monoemulsifier is food-grade PGPR.

[0018] Preferably, the oil phase (O) is at least one of lard, coconut oil, palm oil, or lauric acid.

[0019] Meanwhile, this invention provides a method for preparing the microcapsules, comprising the following steps: S1. Mix the oil phase (O) and the internal aqueous phase (W1) at a volume ratio of 1:4~5, and then add the emulsifier; S2. The oil phase (O), the internal aqueous phase (W1), and the emulsifier are subjected to shearing and stirring for 1-30 minutes to obtain a W1 / O emulsion; S3. After adding the external aqueous phase (W2) and emulsifier to the W1 / O emulsion in sequence, the shearing and stirring operation is performed to obtain microcapsules.

[0020] Preferably, the shearing speed of the shearing and stirring is 4000~15000 r / min.

[0021] Preferably, the mass-to-volume ratio of the emulsifier to the oil phase (O) is 1~2g:100mL.

[0022] Preferably, the internal aqueous phase (W1) contains dissolved water-soluble functional components, including at least one of vitamin B1, vitamin C, probiotics, and alkaline solution.

[0023] Preferably, the mass ratio of the water-soluble functional component to the internal aqueous phase (W1) is 0.1~2:100.

[0024] Finally, an application of human body temperature-responsive microcapsules is provided, for use in food packaging, drug sustained release, or cosmetics.

[0025] Using PGPR as the sole emulsifier demonstrates that PGPR not only stabilizes the O1 / W / O2 system, but also, through process design, can be used as a single emulsifier to construct highly stable W1 / O / W2 type dual emulsions.

[0026] The two-step method in this application is not a traditional two-step method, but a functionally oriented innovation that combines traditional methods with temperature-responsive release requirements. The first step, the shear-stirring unit, is primarily designed for high-melting-point, easily crystallizing oil phases to obtain high-quality W1 / O primary emulsions. The second step, the shear-stirring unit, aims to form W1 / O / W2 microcapsules with regular structures, high encapsulation rates, and controllable release behavior. The entire process is closely integrated with the selected oil phase having a specific phase transition temperature range and the temperature-responsive release function, forming an inseparable whole.

[0027] The prepared microcapsules are solid at room temperature, exhibiting good stability and facilitating storage and transportation. When the temperature reaches near human body temperature, they can rapidly and controllably release the encapsulated active ingredients. This solid-state storage-liquid-release characteristic offers irreplaceable advantages in protecting sensitive components such as probiotics and vitamins, as well as in achieving targeted drug release and timed release of food flavors.

[0028] In one embodiment, the method for preparing human body temperature-responsive microcapsules provided in Embodiment 1 of the present invention, such as... Figure 1 As shown, it includes the following steps: S1. Mix the oil phase (O) and the internal aqueous phase (W1) at a volume ratio of 1:4~5, and then add the emulsifier.

[0029] Specifically, 4 mL of 50°C lard and 1 mL of 0.1 mol / L sodium hydroxide are added to an emulsification container at a volume ratio of 1:5, and then 0.08 g of polyglycerol ricinoleate (PGPR) emulsifier is added to the emulsification container.

[0030] S2. The oil phase (O), the internal aqueous phase (W1), and the emulsifier are sheared and stirred for 1 to 30 minutes to obtain a W1 / O emulsion.

[0031] Specifically, the three substances in the S1 emulsification container are sheared and stirred for 2 minutes at a high speed of 10,000 r / min at 50°C to obtain a W1 / O emulsion containing dispersed droplets.

[0032] S3. After adding the external aqueous phase (W2) and emulsifier to the W1 / O emulsion in sequence, the shearing and stirring operation is performed to obtain microcapsules.

[0033] Specifically, 2 mL of the W1 / O emulsion was used as the oil phase in the second step, and then added together with 4 mL of water and 0.10 g of PGPR into an emulsification container. Under conditions of 50°C, the three substances in the emulsification container were sheared and stirred at a high speed of 6000 r / min for 2 min to prepare a W1 / O / W2 microcapsule emulsion with human body temperature responsive release function.

[0034] like Figure 2 As shown, the prepared microcapsule emulsion is milky white. The emulsion does not break down after being stored at room temperature for 3 months, indicating that the prepared emulsion has good stability. The emulsion does not flow when inverted at room temperature, indicating that the internal oil phase lard crystallizes and solidifies.

[0035] like Figure 3 As shown in the image, a fluorescence microscope image of the double emulsion is displayed. The oil phase is stained red with a fluorescent dye. The microscopic image clearly shows that there are black oil phase areas inside and outside the droplets formed by the red oil phase, which directly proves that the obtained emulsion is a W1 / O / W2 type double emulsion. The droplets are independent of each other in the oil phase and there is no aggregation phenomenon, indicating that the emulsifier plays a good stabilizing role.

[0036] The schematic diagram of the prepared W1 / O / W2 type dual emulsion and the schematic diagram of the simulated fluorescence microscope clearly show the two-film three-cavity structure of the prepared dual emulsion, namely the innermost black inner aqueous phase, the middle red oil phase, and the outermost black outer aqueous phase.

[0037] like Figure 4 The figure shows the particle size distribution of the prepared emulsion droplets. The average particle size of the emulsion droplets is about 17 μm, indicating that the PGPR emulsification and dispersion effect is very good.

[0038] like Figure 5 As shown in (a), the prepared microcapsule emulsion is solid at room temperature and can be inverted at the bottom of a bottle or hung on a vertical carrier plate; Figure 5As shown in (b), the solid microcapsule emulsion can be scraped into a phenolphthalein solution at room temperature; as Figure 5 As shown in (c), at room temperature, sodium hydroxide in the aqueous phase inside the microcapsule emulsion is confined inside and cannot be released into the phenolphthalein solution; Figure 5 As shown in (d), when the temperature rises to around 37°C (human body temperature), the solid lard in the middle layer of the microcapsule melts and releases sodium hydroxide from the internal aqueous phase. The sodium hydroxide reacts with the phenolphthalein solution to produce a color change, turning the transparent phenolphthalein solution red. This demonstrates that the prepared microcapsule can respond to the human body temperature by releasing the sodium hydroxide solution loaded in the internal aqueous phase.

[0039] In one embodiment, the method for preparing a human body temperature-responsive microcapsule provided in Embodiment 2 of the present invention includes the following steps: S1. Add 4 mL of the oil phase (O) obtained by mixing 50°C lard and coconut oil (volume ratio 1:1) and 1 mL of the water phase (W1) containing 1 g / 100 g vitamin C to the emulsification container at a volume ratio of 1:4. Then add 0.08 g of emulsifier PGPR to the emulsification container.

[0040] S2. The three substances in the emulsification container of S1 are sheared and stirred at 12000 r / min at 50°C for 15 min to obtain a milky white W1 / O emulsion. S3. Take 2 mL of the W1 / O emulsion obtained in step S2 as the oil phase, add 4 mL of deionized water and 0.10 g of PGPR, mix to form the second emulsion system, and shear and stir at 8000 r / min for 20 min at 50°C to obtain the W1 / O / W2 microcapsule emulsion with human body temperature responsive release performance.

[0041] The resulting microcapsule emulsion was a milky white semi-solid at room temperature, with a particle size distribution ranging from 10 to 30 μm and an average particle size of approximately 18 μm. Stability testing showed no significant stratification or demulsification after 60 days at room temperature. Upon heating to 37°C, the internal lard and coconut oil layers melted, and vitamin C was rapidly released, verifying that this microcapsule system exhibits temperature-responsive release characteristics.

[0042] In one embodiment, the method for preparing a human body temperature-responsive microcapsule provided in Embodiment 3 of the present invention includes the following steps: S1. Take 4 mL of 50°C palm oil phase (O) and mix it with 1 mL of a solution containing 1×10⁻⁶ ppm. 8 The aqueous phase (W1) of CFU / mL lactic acid bacteria was added to the emulsification container at a volume ratio of 1:5, and then 0.10g of emulsifier PGPR was added to the emulsification container. S2. The three substances in the emulsification container of S1 are sheared and stirred at 12000 r / min for 10 min at 50°C to form a stable W1 / O emulsion. S3. Take 2 mL of the W1 / O emulsion obtained in step S2 as the oil phase, add 4 mL of deionized water and 0.08 g of PGPR to form the second step system, and shear and stir at 6000 r / min for 15 min at 45°C to obtain the W1 / O / W2 dual emulsion type microcapsule emulsion.

[0043] The resulting microcapsule emulsion was milky white with an average particle size of about 20 μm. When heated to 37°C, the palm oil melted, releasing the internal probiotic aqueous phase, indicating that the system can effectively protect probiotics and achieve temperature-responsive release.

[0044] In one embodiment, the method for preparing a human body temperature-responsive microcapsule provided in Embodiment 4 of the present invention includes the following steps: S1. Take 4 mL of 50°C lard mixed with lauric acid (volume ratio 3:1) oil phase (O), 1 mL of 0.1 mol / L vitamin B1 (W1) and 0.09 g PGPR and add them to the emulsification container. S2. The three substances in the emulsification container of S1 are sheared and stirred at 10000 r / min for 20 min at 50°C to obtain W1 / O emulsion containing orange-yellow dispersed droplets. S3. Take 2 mL of W1 / O emulsion as the second oil phase, add 4 mL of deionized water and 0.12 g of PGPR to form the second system. Shear and stir at 7000 r / min for 10 min at 50°C to obtain W1 / O / W2 type microcapsule emulsion with human body temperature response release function.

[0045] The resulting microcapsule emulsion was a lightly uniformly dispersed system with an average particle size of approximately 15 μm. No significant stratification or oil separation was observed after 3 months of storage. When heated to 37°C, the oil phase transitioned from a solid to a liquid state, and vitamin B1 was completely released into the aqueous phase within 10 minutes, indicating that the system exhibits excellent temperature-responsive release performance.

[0046] In one comparative example, Comparative Example 1 of the present invention provides a method for preparing a human body temperature-responsive microcapsule, comprising the following steps: S1. Add 4 mL of the oil phase (O) obtained by mixing 50°C lard and coconut oil (volume ratio 1:1) and 1 mL of the water phase (W1) containing 1 g / 100 g vitamin C to the emulsification container at a volume ratio of 1:4. Then add 0.08 g of emulsifier PGPR to the emulsification container.

[0047] S2. The three substances in the emulsification container of S1 are sheared and stirred at 12000 r / min at 50°C for 15 min to obtain a milky white W1 / O emulsion. S3. Take 2 mL of the W1 / O emulsion obtained in step S2 as the oil phase, add 4 mL of deionized water and 0.12 g of sodium dodecyl sulfate, mix to form the second emulsion system, and shear and stir at 8000 r / min for 20 min at 50°C to obtain the W1 / O / W2 microcapsule emulsion with human body temperature responsive release performance.

[0048] The optical and fluorescence microscopy results of the obtained microcapsule emulsion are as follows: Figure 6 As shown, many small spheres can be clearly seen inside the oil droplet under an optical microscope. Further evidence from a fluorescence microscope confirms that the small spheres inside the red oil droplet are non-fluorescent aqueous phases, verifying the formation of a double emulsion. The droplet size distribution ranges from 20 to 40 μm, with an average size of about 29 μm.

[0049] Compared with Example 2, the microcapsule emulsion obtained in this comparative example has a larger average particle size (29 μm vs 18 μm) and a wider particle size distribution. It also exhibits slight stratification after 60 days of storage at room temperature, indicating that the use of a non-PGPR emulsifier in the second step may have an adverse effect on the long-term stability and dispersion uniformity of the emulsion.

[0050] In one comparative example, Comparative Example 2 of the present invention provides a method for preparing a human body temperature-responsive microcapsule, comprising the following steps: S1. Add 4 mL of the oil phase (O) obtained by mixing 50°C lard and coconut oil (volume ratio 1:1) and 1 mL of the water phase (W1) containing 1 g / 100 g vitamin C to the emulsification container at a volume ratio of 1:4. Then add 0.08 g of sunflower phospholipid emulsifier to the emulsification container.

[0051] S2. The three substances in the emulsification container of S1 are sheared and stirred at 12000 r / min at 50°C for 15 min to obtain a milky white W1 / O emulsion. S3. Take 2 mL of the W1 / O emulsion obtained in step S2 as the oil phase, add 4 mL of deionized water and 0.11 g of Tween80, mix to form the second emulsion system, and shear and stir at 8000 r / min for 20 min at 50°C to obtain the W1 / O / W2 microcapsule emulsion with human body temperature responsive release performance.

[0052] The optical microscope image of the obtained microcapsule emulsion is as follows: Figure 7As shown, many small spheres can be clearly seen inside the oil phase droplets under an optical microscope, verifying the formation of the double emulsion. The droplet size distribution ranges from 30 to 50 μm, with an average size of about 36 μm.

[0053] Comparative Example 2 requires the use of two emulsifiers with different HLB values ​​(sunflower phospholipid and Tween 80), necessitating the screening and matching of emulsifiers, which contradicts the simplified process principle of this invention. The resulting emulsion had an average particle size (36 μm) significantly larger than that of Examples 1-4, and temperature-response release tests showed that the complete release time of vitamin C was approximately 50% longer than in Example 2, indicating slightly poorer release controllability.

[0054] In one comparative example, Comparative Example 3 of the present invention provides a method for preparing an oil-in-water-in-oil (O / W / O) dual emulsion using a single emulsifier in a one-step process. The specific preparation steps are as follows: S1. Add dodecane and water to the emulsion bottle at a volume ratio of 3.2 mL: 2.8 mL; S2. Add 0.06g of polyglycerol ricinoleate (PGPR) emulsifier to the emulsification bottle from step S1; S3. The three substances in the emulsion bottle in step S2 are processed in one step under high-speed homogenization shearing conditions of 8000 r / min and shearing and stirring for 6 min to obtain an emulsion containing dispersed droplets; that is, an O / W / O dual emulsion is formed.

[0055] like Figure 8 As shown in the image, a fluorescence microscope image of the double emulsion is displayed. The aqueous phase is stained green with a fluorescent dye. The microscopic image clearly shows black oil phase regions inside and outside the droplets formed by the green aqueous phase, which directly proves that the obtained emulsion is an oil-in-water-in-oil double emulsion. However, this method is only applicable to organic alkane systems such as dodecane. Moreover, when extending to temperature-controlled release W1 / O / W2 double emulsions, there are not only structural limitations, but also great difficulty in obtaining a primary W / O emulsion with uniform, complete, and stable internal aqueous phase droplets for phase change oils that solidify at room temperature. This results in the inability to form W1 / O / W2 microcapsules with regular structure, high encapsulation rate, and controllable release behavior.

[0056] Comparative Example 3 confirms that the one-step PGPR single emulsifier method disclosed in prior art CN120678725A is only applicable to O1 / W / O1 type systems and low-melting-point oil phases (such as dodecane). When directly applied to the W1 / O / W2 type system described in this invention and using phase change greases (such as lard), a stable primary W / O emulsion cannot be obtained, and thus, it is completely impossible to form well-defined microcapsules with temperature-responsive release function. This highlights that the "two-step" process designed for specific systems in this invention is indispensable and not obvious.

[0057] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention (including the claims) is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in the details for the sake of brevity.

[0058] This invention is intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. A human body temperature-responsive microcapsule, characterized in that, The microcapsules are of type W1 / O / W2, wherein, Internal aqueous phase (W1): Contains releasable functional components; Oil phase (O): Coated outside the inner aqueous phase (W1), the oil phase (O) has solid-liquid phase transition characteristics at 25℃~40℃, which enables the microcapsules to release the functional components in the inner aqueous phase (W1) when the oil phase (O) undergoes a phase transition. External aqueous phase (W2): Coated outside the intermediate oil phase (O); The W1 / O / W2 system also includes an emulsifier for stabilizing the W1 / O interface and the O / W2 interface.

2. The microcapsule according to claim 1, characterized in that, The emulsifier is a monoemulsifier.

3. The microcapsule according to claim 2, characterized in that, The monoemulsifier is food-grade PGPR.

4. The microcapsule according to claim 1, characterized in that, The oil phase (O) is at least one of lard, coconut oil, palm oil or lauric acid.

5. A method for preparing a human body temperature-responsive microcapsule, characterized in that, Includes the following steps: S1. Mix the oil phase (O) and the internal aqueous phase (W1) at a volume ratio of 1:4~5, and then add the emulsifier; S2. The oil phase (O), the internal aqueous phase (W1), and the emulsifier are subjected to shearing and stirring for 1-30 minutes to obtain a W1 / O emulsion; S3. After adding the external aqueous phase (W2) and emulsifier to the W1 / O emulsion in sequence, the shearing and stirring operation is performed to obtain microcapsules.

6. The preparation method according to claim 5, characterized in that, The shearing speed of the shearing and stirring is 4000~15000 r / min.

7. The preparation method according to claim 5, characterized in that, The mass-to-volume ratio of the emulsifier to the oil phase (O) is 1~2g:100mL.

8. The preparation method according to claim 5, characterized in that, The internal aqueous phase (W1) contains dissolved water-soluble functional components, including at least one of vitamin B1, vitamin C, and probiotics.

9. The preparation method according to claim 5 or 8, characterized in that, The mass ratio of the water-soluble functional component to the internal aqueous phase (W1) is 0.1~2:

100.

10. The application of the human body temperature-responsive microcapsule as described in claim 1, characterized in that, It can be used in food packaging, drug release formulations, or cosmetics.

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