Efficient air eDNA collection and extraction method and application
By optimizing the air eDNA collection and extraction process, using glass fiber membranes, PBS buffer elution, and PES membrane enrichment, the problem of insufficient DNA quality in air eDNA collection and extraction was solved, achieving efficient DNA extraction and accurate subsequent analysis.
CN122344572APending Publication Date: 2026-07-07GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Patent Information
- Application Number
- CN202610502774.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-16
- Publication Date
- 2026-07-07
AI Technical Summary
Technical Problem
Existing air eDNA collection and extraction technologies struggle to obtain high-quality DNA, leading to unsatisfactory results in subsequent experiments.
Method used
Sampling was performed using glass fiber membranes, combined with PBS buffer elution, cryopreservation, low-speed centrifugation, and PES membrane enrichment. The sampling and extraction process was optimized, including treatment with proteinase K and sodium dodecyl sulfate to ensure DNA integrity and concentration.
Benefits of technology
It significantly improves the extraction quality and concentration of airborne eDNA, ensuring the accuracy of subsequent experimental analysis. It is applicable to a variety of atmospheric environments and has a wide range of applications.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses an efficient air eDNA collection and extraction method and application. According to the method, a glass fiber filter membrane is used as a sampling filter membrane, a medium-flow particulate matter sampler and a TSP sampling head are used for sampling, dry ice is used for storing and transporting a sample, a PBS buffer solution and low-temperature low-speed centrifugation are used for eluting DNA, a PES filter membrane is used for enriching and precipitating particulate matter, and the PES filter membrane is cut into small blocks to improve the DNA extraction efficiency. The environmental DNA extracted by adopting the scheme of the invention has higher concentration and good integrity, and meanwhile, the DNA extracted by utilizing the method is amplified by adopting universal primers of different species, so that a good amplification result can be obtained, and the subsequent eDNA macro bar code technology is convenient to carry out species diversity analysis.
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