Method for quantitatively detecting antibiotics in soil based on LC-MS / MS

By combining LC-MS/MS with freeze-drying and treatment with specific masking agents, the complexity of detecting multiple antibiotics in soil has been solved, enabling simplified operation, low sample size, and high sensitivity for quantitative detection of multiple categories, applicable to complex matrices such as soil, feces, and sludge.

CN122361669APending Publication Date: 2026-07-10NANJING ADVANCED ACAD OF LIFE & HEALTH
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Patent Information

Application Number
CN202610657659.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-13
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing technologies for detecting antibiotic residues in soil suffer from problems such as cumbersome and complex sample pretreatment procedures, large sample volumes, low detection throughput, limited applicability to a single type of antibiotic, and insufficient method stability, making it difficult to achieve simultaneous and accurate quantitative detection of multiple types of antibiotics.

Method used

A detection method based on LC-MS/MS, combined with freeze-drying, micro-sampling, and liquid chromatography-tandem mass spectrometry, simplifies the pretreatment process and uses specific masking agents and internal standards to correct matrix effects, enabling efficient quantitative detection of multiple classes of antibiotics.

Benefits of technology

It achieves simplified pretreatment operations, low sample volume detection, improved detection sensitivity and accuracy, is suitable for the quantification of various types of antibiotics, reduces the amount of organic solvent used, and is applicable to complex matrices such as soil, animal feces and sludge.

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Abstract

The invention discloses a method for quantitatively detecting antibiotics in soil based on LC-MS / MS. The method comprises the following steps: freeze-drying a soil sample, weighing a trace amount of freeze-dried powder, adding an extracting solution, carrying out vortex and ultrasonic-assisted extraction, centrifuging to take a supernatant, carrying out nitrogen blowing concentration until the supernatant is dried, redissolving and filtering, and carrying out qualitative and quantitative analysis by adopting a high performance liquid chromatography-tandem mass spectrometry method. The method does not need a solid-phase extraction column purification step, the pretreatment operation is simple and efficient, the sample dosage is only 0.1 g, the sensitivity is high, the quantitation limit is low, and an internal standard method is adopted to correct the matrix effect and the linear correlation coefficient R2gt; the adding standard recovery rate is stabilized at 80-120%, and the method is accurate and reliable. The method can be generally used for synchronous or respective quantitative detection of more than twenty kinds of antibiotics such as chloramphenicol, tetracycline, quinolones and macrolides in soil, can be directly expanded and applied to complex environment matrixes such as animal waste and sludge, is low in organic solvent consumption, and is green and environment-friendly.
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