Fluid loading module, system, method, and genetic sequencing device

Abstract

A fluid loading module, fluid loading system, fluid loading method, and gene sequencing apparatus are disclosed for loading fluid onto a solid-phase slide. The solid-phase slide includes multiple openings. The fluid loading system includes a manifold block and a valve device. The manifold block has a first flow channel and is provided with a first fluid inlet, a second fluid inlet, a first flow channel outlet, and multiple slide interfaces. The first fluid inlet and the first flow channel outlet are both connected to the first flow channel. Each slide interface has a first hole, and the first hole of each slide interface is aligned with an opening of the solid-phase slide. The valve device includes multiple first valves. Each first valve is connected to the first flow channel and the first hole of a slide interface, allowing a first fluid inlet to connect to a corresponding slide interface through a first valve. A second fluid inlet connects to another slide interface.

Description

Fluid loading module, system, method and gene sequencing device Technical Field

[0001] The present application relates to the field of biological sample loading, and in particular to a fluid loading module, a fluid loading system having the fluid loading module, a fluid loading method applied to the fluid loading system, and a gene sequencing device. Background Art

[0002] The primary goal of liquid spreading techniques on solid surfaces is to evenly distribute the liquid across the surface of the solid phase carrier. For carriers with open spaces, spreading techniques include spraying, atomization deposition, coating, and dipping. The uniformity and purity of these techniques depend primarily on the external environment. For carriers with enclosed spaces, particularly channel-type carriers with inlets and outlets, external forces hinder access to the interior of the channels. Therefore, the performance of the fluidic module supporting these inlets and outlets becomes the dominant factor in liquid spreading.

[0003] In related technologies, the fluidics module includes a manifold plate that cooperates with a solid-phase carrier to load liquid onto the carrier to achieve uniform liquid distribution within the flow channels within the carrier. However, the openings in the manifold plate are generally used for only one-way liquid inlet or outlet, resulting in a single liquid distribution method and limited flexibility.

[0004] Summary of the Invention

[0005] In view of this, it is necessary to propose a fluid loading module, a fluid loading system, a fluid loading method and a gene sequencing device.

[0006] In a first aspect, the present application provides a fluid loading module for loading fluid onto a solid phase carrier. The solid phase carrier includes a plurality of openings. The fluid loading system includes a manifold block and a valve device. A first flow channel is provided in the manifold block, and a first fluid inlet, a second fluid inlet, a first flow channel outlet and a plurality of carrier interfaces are provided on the manifold block. The first fluid inlet and the second fluid inlet are both used to connect to a first fluid source. The first fluid inlet and the first flow channel outlet are both connected to the first flow channel. The carrier interface is provided with a first hole, and the first hole of each carrier interface is used to dock with one of the openings of the solid phase carrier. The valve device includes a plurality of first valves, each of the first valves being connected to the first flow channel and the first hole of a carrier interface. Each of the first valves is used to connect or disconnect the first flow channel and the first hole, so that the first fluid inlet can be connected to the corresponding carrier interface through a first valve. The second fluid inlet is connected to another carrier interface.

[0007] A second aspect of the present application provides a fluid loading system, comprising the above-mentioned fluid loading module and a first fluid source. The first fluid source is used to store at least one first fluid, and the first fluid source is connected to the first fluid inlet and the second fluid inlet of the fluid loading module.

[0008] A third aspect of the present application provides a gene sequencing device, comprising the above-mentioned fluid loading module or fluid loading system.

[0009] The fourth aspect of the present application provides a fluid loading method, which is applied to the above-mentioned fluid loading module. The fluid loading method includes: installing the solid phase carrier on the manifold block, the solid phase carrier including multiple openings, so that the first hole of each carrier interface is sealed and docked with one of the openings of the solid phase carrier; connecting the first fluid inlet and the second fluid inlet to a first fluid source respectively, the first fluid source storing at least one first fluid; controlling the first valve corresponding to the first fluid inlet to be turned on, and introducing the first fluid to the connected carrier interface through the first fluid inlet, so that the first fluid enters one of the openings and flows out from at least one other opening; introducing the first fluid to the connected carrier interface through the second fluid inlet, so that the first fluid enters another of the openings and flows out from at least one other opening.

[0010] In the present application, at least one wafer interface of the manifold block can be used not only as an inlet but also as an outlet. The loading direction and loading sequence of the fluid can be made flexible by the control of the valve device, thereby achieving efficient fluid loading and fluid replacement and avoiding cross contamination between different fluids. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] FIG1A is a schematic diagram of the structure of a flow cell slide provided in one embodiment of the present application.

[0012] FIG1B is a schematic diagram of the structure of a flow cell slide provided in another embodiment of the present application.

[0013] FIG1C is a schematic diagram of the structure of a flow cell slide provided in another embodiment of the present application.

[0014] FIG2 is a schematic structural diagram of a fluid loading system provided in one embodiment of the present application.

[0015] FIG3 is a module architecture diagram of the fluid loading system shown in FIG2 .

[0016] FIG. 4 is a schematic structural diagram of a manifold block of the fluid loading system shown in FIG. 2 .

[0017] FIG. 5 is a diagram showing the internal structure of the manifold block shown in FIG. 4 .

[0018] 6 is a schematic diagram illustrating the flow cell slide shown in FIG. 1A , FIG. 1B , or FIG. 1C after being placed on the manifold block shown in FIG. 5 .

[0019] 7A is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG. 6 in the first working mode.

[0020] 7B is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG. 6 in the second operating mode.

[0021] 7C is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG. 6 in the third operating mode.

[0022] 7D is a schematic diagram illustrating the fluid flow within the manifold block and flow cell slide shown in FIG6 in a fourth operating mode.

[0023] 7E is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG6 in the fifth working mode.

[0024] 7F is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG. 6 in the sixth operating mode.

[0025] 7G is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG6 in the seventh working mode.

[0026] 7H is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG6 in the eighth operating mode.

[0027] FIG7I is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG6 in the ninth working mode.

[0028] 7J is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG6 in the tenth working mode.

[0029] FIG7K is a schematic diagram of the fluid flow direction in the manifold block and flow cell slide shown in FIG6 in the eleventh working mode.

[0030] FIG8 is a flow chart of a fluid loading method provided in one embodiment of the present application.

[0031] FIG9 is a module architecture diagram of a gene sequencing device provided in one embodiment of the present application.

[0032] Description of main component symbols

[0033] Flow cell slide 1

[0034] First opening 1a

[0035] Second opening 1b

[0036] The third opening 1c

[0037] Fourth opening 1d

[0038] First side 1A

[0039] Second side 1B

[0040] Third side 1C

[0041] Fourth side 1D

[0042] first trench 1e

[0043] Second trench 1f

[0044] Support platform 10

[0045] Fixing device 11

[0046] Manifold block 20

[0047] First flow channel 20a

[0048] Section 1 20a1

[0049] Paragraph 2, 20a2

[0050] Second flow channel 20b

[0051] First fluid inlet 21, 21A, 21B, 21C

[0052] Second fluid inlet 22

[0053] The third fluid inlet 23

[0054] First fluid outlet 24

[0055] Second fluid outlet 25

[0056] First connection port 26

[0057] Second connection port 27

[0058] Slide interface 28

[0059] First hole 28a

[0060] Second hole 28b

[0061] Seal 29

[0062] The third hole 29a

[0063] Fourth hole 29b

[0064] Slide mounting table 30

[0065] Valve device 40

[0066] First valve 41

[0067] Second valve 42

[0068] Processor 43

[0069] First temperature control module 50

[0070] Second temperature control module 60

[0071] Fluid loading module 100

[0072] Fluid storage module 101

[0073] First fluid source 101a

[0074] Second fluid source 101b

[0075] First power source 101c

[0076] Waste liquid collection module 102

[0077] First waste liquid device 102a

[0078] Second waste liquid device 102b

[0079] Second power source 102c

[0080] Fluid loading system 200

[0081] Gene sequencing device 300

[0082] Fluid loading module 100

[0083] Base 1001

[0084] Steps S1-S4

[0085] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0086] The following will clearly and completely describe the technical solutions in the embodiments of the present application in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0087] It should be noted that when a component is referred to as being "fixed to" or "mounted on" another component, it may be directly on the other component or there may be a central component. When a component is referred to as being "disposed on" another component, it may be directly on the other component or there may be a central component. As used herein, the term "and / or" includes all and any combinations of one or more of the relevant listed items.

[0088] It should be noted that, although a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in an order different from that in the flowchart. The method disclosed in the embodiments of the present application includes one or more steps or actions for implementing the method. The method steps and / or actions may be interchangeable with each other without departing from the scope of the claims. Unless a specific order of steps or actions is specified, the order and / or use of specific steps and / or actions may be modified without departing from the scope of the claims.

[0089] Please refer to Figure 1A. One embodiment of the present application provides a flow cell carrier 1. The flow cell carrier 1 has a flow channel (not shown) inside. The flow cell carrier 1 includes a substrate 1001 and a cover plate (not shown) covering the surface of the substrate 1001, and the flow channel is arranged between the cover plate and the substrate 1001. At least two openings are provided on the substrate 1001, and the fluid can flow into or out of the flow cell carrier 1 through each opening. Except for the above-mentioned openings, the rest of the flow cell carrier 1 is isolated from the atmosphere. Among them, at least one opening can serve as an inlet for the inflow of fluid, and can also serve as an outlet for the outflow of fluid. Therefore, the inlet and outlet for the fluid to be loaded into the flow cell carrier 1 are variable, and the fluid loading direction and loading order are variable.

[0090] In some embodiments, the flow cell slide 1 is roughly square, including a first side 1A, a second side 1B, a third side 1C and a fourth side 1D connected in sequence, and the flow cell slide 1 has a square flow channel inside. Four openings are provided at the four corners of the substrate 1001. For ease of description, the four openings are named as the first opening 1a, the second opening 1b, the third opening 1c and the fourth opening 1d. The first opening 1a is located at the corner between the first side 1A and the fourth side 1D, the second opening 1b is located at the corner between the second side 1B and the third side 1C, the third opening 1c is located at the corner between the first side 1A and the second side 1B, and the fourth opening 1d is located at the corner between the third side 1C and the fourth side 1D. The first opening 1a is adjacent to the third opening 1c, and the line connecting the first opening 1a and the second opening 1b coincides with a diagonal line of the flow cell slide 1, and the line connecting the third opening 1c and the fourth opening 1d coincides with another diagonal line of the flow cell slide 1. The fluid can flow through the flow cell slide 1 in a single-input and single-output manner, a single-input and multiple-output manner, multiple-input and single-output manner, or multiple-input and multiple-output manners. For example, the fluid can flow into the flow cell slide 1 through the first opening 1a and then flow out through the second opening 1b, thereby achieving a single-input and single-output manner; the fluid can also flow into the flow cell slide 1 through the first opening 1a and then flow out through the second opening 1b, the third opening 1c, and the fourth opening 1d, thereby achieving a single-input and multiple-output manner; the fluid can also flow into the flow cell slide 1 through the first opening 1a and the second opening 1b and then flow out through the third opening 1c and the fourth opening 1d, thereby achieving multiple-input and multiple-output manners. In some embodiments, the gap between the substrate 1001 and the cover sheet can be set to 20 microns to 200 microns. Either the length or width of the flow cell slide 1 is 3 mm to 200 mm.

[0091] Referring to FIG. 1B , in some other embodiments, a first groove 1e is defined between the first opening 1a and the third opening 1c. The first groove 1e serves to drain liquid during the spreading process of the flow cell slide 1, thereby improving the uniformity of the spread. The first groove 1e can be elongated and can have a depth of 0.1 mm to 2 mm.

[0092] Referring to FIG. 1C , in some further embodiments, a second groove 1f may be defined between the second opening 1b and the fourth opening 1d. The second groove 1f serves to drain liquid during the spreading process of the flow cell slide 1, thereby improving the uniformity of the spread. The second groove 1f may be elongated and have a depth of 0.1 mm to 2 mm.

[0093] Referring to Figures 2 and 3, one embodiment of the present application further provides a fluid loading system 200. The fluid loading system 200 includes a fluid storage module 101, a fluid loading module 100, and a waste liquid collection module 102. The fluid loading module 100 is used to mount a flow cell slide 1 or other solid phase slide. The fluid storage module 101 is connected upstream of the fluid loading module 100, and is used to provide fluid to the fluid loading module 100 and the flow cell slide 1 mounted on the fluid loading module 100. The waste liquid collection module 102 is connected downstream of the fluid loading module 100, and is used to collect waste liquid flowing out of the fluid loading module 100 and the flow cell slide 1 mounted on the fluid loading module 100.

[0094] The fluid loading module 100 includes a support platform 10, a manifold block 20 provided on the support platform 10, a slide mounting platform 30 provided on the manifold block 20, and a valve device 40 connected to the manifold block 20. The slide mounting platform 30 can be provided in the middle of the manifold block 20 for mounting the flow cell slide 1. The manifold block 20 is in communication with the fluid storage module 101, and the manifold block 20 is used to load the fluid stored in the fluid storage module 101 into the manifold block 20 or the flow channel of the flow cell slide 1. In some embodiments, the slide mounting platform 30 can use vacuum adsorption to adsorb the flow cell slide 1 onto the slide mounting platform 30. A fixing device 11 can be provided on the support platform 10, and the manifold block 20 can be fixed to the support platform 10 by the fixing device 11. The fixing device 11 can fix the manifold block 20 by mechanical fastening methods such as clamping with a block, locking with a screw, etc.

[0095] In some embodiments, the fluid loading system 200 may further include a first temperature control module 50 and a second temperature control module 60. The first temperature control module 50 may be located below the slide mounting platform 30 and is used to maintain a suitable temperature for the fluid within the flow cell slide 1 when needed. The fluid storage module 101 may also be connected to the second temperature control module 60, which is used to maintain a suitable temperature for the fluid within the fluid storage module 101 when needed, thereby enabling preheating, cooling, or pre-reaction of the fluid within the fluid storage module 101. The aforementioned pre-reaction refers to the conversion of a fluid (e.g., a reagent) into another reagent after temperature incubation and reaction. Thus, the fluid storage module 101 can deliver a fluid at a specific temperature to the fluid loading module 100 and the flow cell slide 1 mounted on the fluid loading module 100, or deliver a specific fluid obtained after a pre-reaction to the fluid loading module 100 and the flow cell slide 1 mounted on the fluid loading module 100. The first temperature control module 50 and the second temperature control module 60 may be a TEC (thermal cooler) or other temperature-controllable device.

[0096] Referring to Figures 4 to 6 , a first flow channel 20a and a second flow channel 20b are defined within the manifold block 20. In some embodiments, the first flow channel 20a may be located outside the second flow channel 20b. As shown in Figure 6 , the first flow channel 20a extends sequentially along the fourth side 1D, the third side 1C, and the second side 1B. The second flow channel 20b extends sequentially along the fourth side 1D, the first side 1A, and the second side 1B.

[0097] The manifold block 20 is also provided with at least one first fluid inlet 21, a second fluid inlet 22, and a third fluid inlet 23. Both the first fluid inlet 21 and the third fluid inlet 23 are connected to the first flow channel 20a. Thus, the fluid stored in the fluid storage module 101 can enter the first flow channel 20a within the manifold block 20 through the first fluid inlet 21 or the third fluid inlet 23. The manifold block 20 is also provided with a first fluid outlet 24 connected to the first flow channel 20a and a second fluid outlet 25 connected to the second flow channel 20b. Thus, the fluid in the first flow channel 20a can flow out of the manifold block 20 through the first fluid outlet 24, and the fluid in the second flow channel 20b can flow out of the manifold block 20 through the second fluid outlet 25.

[0098] The manifold block 20 is also provided with a first connection port 26 and a plurality of second connection ports 27. The valve device 40 includes a plurality of first valves 41 and a second valve 42. Each first valve 41 is connected to the first flow channel 20 via the second connection port 27. The second valve 42 is connected to the first flow channel 20a via the first connection port 26, and is used to control the connection or disconnection of the first flow channel 20a itself, thereby guiding the flow direction of the fluid. In some embodiments, the plurality of first valves 41 can be in a one-to-one correspondence with the plurality of openings of the flow cell slide 1. As shown in Figures 5 and 6, taking the flow cell slide 1 shown in Figure 1A, Figure 1B or Figure 1C as an example, the number of first valves 41 is four, corresponding to the four openings of the flow cell slide 1 respectively. Further, in some embodiments, the second valve 42 is arranged on the first flow channel 20a, and with the second valve 42 as a reference, the first flow channel 20a includes a first section 20a1 located before the second valve 42 and a second section 20a2 located after the second valve 42. One first valve 41 is connected to the first section 20a1, and the remaining first valves 41 are connected to the second section 20a2. The first valve 41 and the second valve 42 can be two-way valves, which are controlled on and off by a processor 43. The processor 43 can be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or any conventional processor. It is understood that the first valve 41 and the second valve 42 can also be other types of valves, and this application is not limited thereto.

[0099] The manifold block 20 is also provided with a plurality of slide interfaces 28, and a plurality of slide interfaces 28 are respectively provided with a plurality of openings of the flow cell slide 1, and a plurality of slide interfaces 28 and a plurality of openings of the flow cell slide 1 are in a one-to-one correspondence relationship. Taking the flow cell slide 1 shown in Figure 1A, Figure 1B or Figure 1C as an example, the manifold block 20 is provided with four slide interfaces 28, which respectively correspond to the four openings of the flow cell slide 1. Each first valve 41 is also connected to a slide interface 28 through the first connecting port 26. The first valve 41 is used to control the connection or disconnection between the first flow channel 20a and the slide interface 28, thereby guiding the flow direction of the fluid. As shown in Figures 5 and 6, wherein, a first hole 28a and a second hole 28b are offered in each slide interface 28, the first hole 28a is communicated with the first flow channel 20a by the first valve 41, and the second hole 28b is communicated with the second flow channel 20b. Each slide interface 28 is docked with an opening of the flow cell slide 1 through the first hole 28a. Therefore, when the first valve 41 is turned on, the fluid in the first flow channel 20a can enter the first hole 28a of the slide interface 28 through the first valve 41, and then enter the opening of the flow cell slide 1. Among them, the first fluid inlet 21 is connected to the slide interface 28 connected to the first opening 1a, and the second fluid inlet 22 is connected to the slide interface 28 connected to the second opening 1b. In the present application, when the fluid flows into the flow cell slide 1 through the first opening 1a, it can flow out through the second opening 1b. At this time, the first opening 1a serves as the inlet of the flow cell slide 1, and the second opening 1b serves as the outlet of the flow cell slide 1; when the fluid flows into the flow cell slide 1 through the second opening 1b, it can flow out through the first opening 1a. At this time, the second opening 1b serves as the inlet of the flow cell slide 1, and the first opening 1a serves as the outlet of the flow cell slide 1. Therefore, at least one opening of the flow cell slide 1 can be used not only as an inlet but also as an outlet, so that the fluid loading direction and loading order are variable. In some embodiments, a sealing member 29 may be provided in each slide interface 28, and a third hole 29a and a fourth hole 29b may be provided on the sealing member 29. The third hole 29a is docked with the first hole 28a and the opening of the flow cell slide 1, respectively, and the fourth hole 29b is docked with the second hole 28b. When viewed from the thickness direction of the flow cell slide 1, the first hole 28a and the third hole 29a may overlap, and the second hole 28b and the fourth hole 29b may overlap. In some embodiments, the sealing member 29 is an annular sealing ring adapted to the slide interface 28.

[0100] During use, the manifold block 20 can be used to install or remove the flow cell slide 1 as needed. For example, when spreading liquid on the flow cell slide 1, the flow cell slide 1 needs to be installed on the manifold block 20; during cleaning, one scenario is to keep the flow cell slide 1 on the manifold block 20. In this case, while cleaning the flow channel in the manifold block 20, the openings of the flow cell slide 1 can also be cleaned; another scenario is to remove the flow cell slide 1 from the manifold block 20, or loosen the flow cell slide 1 so that it is in a non-sealed connection with the manifold block 20 (in this case, the flow cell slide 1 can serve as a cover to avoid splashing during the cleaning process). In this case, only the flow channel in the manifold block 20 or the seal 29 on the manifold block 20 is cleaned. Among them, by cleaning the flow channel in the manifold block 20, the openings of the flow cell slide 1, and the seal 29 on the manifold block 20, the fluid residue in the flow channel, opening, and seal 29 can be removed, thereby preventing the residual fluid from affecting the next spreading of liquid.

[0101] Specifically, when spreading the liquid on the flow cell slide 1, the flow cell slide 1 is placed on the slide mounting table 30, so that the openings of the flow cell slide 1 are aligned one by one with the seal 29, and then the seal 29 is pressed. At this time, the third hole 29a and the fourth hole 29b of the seal 29 are isolated but connected to the opening of the flow cell slide 1 (for example, the flow cell slide 1 can be pressed against the seal 29 by the pressing and fixing device 11, thereby achieving a sealed connection between the flow cell slide 1, the seal 29 and the slide interface 28). Thus, each opening of the flow cell slide 1 can be connected to the first flow channel 20a through the third hole 29a of the seal 29, the first hole 28a of the slide interface 28 and the first valve 41, so that the fluid in the first flow channel 20a (such as a reagent or a cleaning solution) can flow into the flow cell slide 1 through a certain opening, then flow through the flow channel in the flow cell slide 1, and then flow out of the flow cell slide 1 and the first flow channel 20a through other openings. When the flow cell carrier 1 is removed from the fluid loading module 100 (alternatively, the fixing device 11 can be loosened to switch the flow cell carrier 1 and the seal 29 to a non-sealed connection state), since the third hole 29a of the seal 29 is connected to the first flow channel 20a through the first hole 28a of the carrier interface 28 and the first valve 41, and the fourth hole 29b of the seal 29 is connected to the third hole 29a and is connected to the second flow channel 20b through the second hole 28b of the carrier interface 28, the fluid in the first flow channel 20a (such as cleaning fluid) can also flow into the second flow channel 20b after passing through the seal 29. By guiding the cleaning fluid to flow into the second flow channel 20b through the seal 29, the residual fluid on the seal 29 can be discharged.

[0102] As shown in Figures 3 and 7A, in some embodiments, the fluid storage module 101 includes a first fluid source 101a and a second fluid source 101b, each of which is independent of the other. The first fluid source 101a is used to store a first fluid (e.g., a reagent), and the second fluid source 101b is used to store a second fluid (e.g., a cleaning solution). The first fluid inlet 21 and the second fluid inlet 22 are both connected to the first fluid source 101a, allowing the first fluid stored in the first fluid source 101a to flow into the first fluid inlet 21 and the second fluid inlet 22. The third fluid inlet 23 is connected to the second fluid source 101b, allowing the second fluid stored in the second fluid source 101b to flow into the third fluid inlet 23. Therefore, the first flow channel 20a is a common flow channel, which is used to flow the first and second fluids at different time periods. However, the second flow channel 20b is only used to flow the second fluid. Furthermore, in some embodiments, the third fluid inlet 23 can also be connected to the first fluid source 101a, so that the third fluid inlet 23 can serve as a common fluid inlet, allowing the first and second fluids to flow in at different time periods. The first fluid source 101a and the second fluid source 101b can be containers suitable for storing and containing fluids, respectively, and this application does not impose any restrictions. The first fluid source 101a and the second fluid source 101b can be independently connected to a first power source 101c, which is used to provide power for the first fluid in the first fluid source 101a or the second fluid in the second fluid source 101b to enter the corresponding fluid inlet. The first power source 101c can be various types of pumps for driving fluid movement, such as a syringe pump, a plunger pump, a diaphragm pump, a gear pump, and a peristaltic pump.

[0103] As shown in FIG5 , in some embodiments, there are multiple first fluid inlets 21. In this embodiment, there are three first fluid inlets 21. For ease of description, the three first fluid inlets 21 are respectively a first fluid inlet 21A, a first fluid inlet 21B, and a first fluid inlet 21C. The first section 20a1 of the first flow channel 20a, facing away from the second section 20a2, is connected to the first fluid inlet 21A and the third fluid inlet 23, respectively. The first fluid inlet 21B and the first fluid inlet 21C are both located in the first section 20a1 of the first flow channel 20a, with the first fluid inlet 21C located between the first fluid inlet 21A and the first fluid inlet 21B. The first fluid inlet 21A, the first fluid inlet 21B, the first fluid inlet 21C, the second fluid inlet 22, and the third fluid inlet 23 are each configured to allow different types of first fluid to flow in. Correspondingly, the first fluid source 101a includes multiple channels (not shown), and the first fluid inlet 21A, the first fluid inlet 21B, the first fluid inlet 21C, the second fluid inlet 22 and the third fluid inlet 23 are respectively connected to different channels of the first fluid source 101a.

[0104] Therefore, different types of first fluids can enter the manifold block 20 through different fluid inlets, respectively, thereby achieving separation of different types of first fluids and avoiding cross-contamination problems. For example, the first fluid stored in a certain channel of the first fluid source 101a can flow into the first fluid inlet 21, and then enter the first opening 1a of the flow cell slide 1 through the first valve 41, the corresponding slide interface 28, and the seal 29; the first fluid stored in another channel of the first fluid source 101a can flow into the second fluid inlet 22, and then enter the second opening 1b of the flow cell slide 1 through the first valve 41, the corresponding slide interface 28, and the seal 29. By setting the fluid path between the first fluid inlet 21 and the connected slide interface 28 and the fluid path between the second fluid inlet 22 and the connected slide interface 28 to not overlap, that is, the first fluid flowing in through the second fluid inlet 22 is staggered with the paths of other first fluids before entering the flow cell slide 1, thereby avoiding cross-contamination problems between different reagents. In actual operation, key sensitive reagents can be selected and directed into the flow cell slide 1 using different fluid inlets of the manifold block 20, thereby avoiding the risk of quality degradation due to cross-contamination. As shown in Figures 5 and 6, in some embodiments, the first fluid inlet 21A and the third fluid inlet 23 can be located near the first side 1A, while the first fluid inlet 21B and the first fluid inlet 21C can be located near the fourth side 1D. The first fluid inlet 21 and the third fluid inlet 23 both pass through the slide interface 28 connected to the first opening 1a and connected to the first valve 41. The second fluid inlet 22 is connected between the first valve 41 corresponding to the second opening 1b and the slide interface 28 connected to the second opening 1b, so that the fluid path between the second fluid inlet 22 and the connected slide interface 28 does not pass through the first valve 41. Therefore, the first fluid can directly enter the slide interface 28 connected to the second opening 1b without passing through the first valve 41, and then enter the second opening 1b. The second fluid inlet 22 can be located near the second side 1B or the third side 1C. Figure 5 shows the second fluid inlet 22 located near the third side 1C.

[0105] In some embodiments, the waste liquid collection module 102 includes a first waste liquid device 102a and a second waste liquid device 102b that are independent of each other. The first waste liquid device 102a is connected to the first fluid outlet 24 and is used to collect waste liquid flowing out of the first fluid outlet 24. The second waste liquid device 102b is connected to the second fluid outlet 25 and is used to collect waste liquid flowing out of the second fluid outlet 25. The first waste liquid device 102a and the second waste liquid device 102b can be independently connected to a second power source 102c, which is used to provide power for the waste liquid to flow into the first waste liquid device 102a and the second waste liquid device 102b. The first waste liquid device 102a and the second waste liquid device 102b can be containers suitable for collecting and holding fluids, respectively, and this application is not limited thereto. The second power source 102c can be various types of pumps for driving fluid movement, such as a syringe pump, a plunger pump, a diaphragm pump, a gear pump, and a peristaltic pump.

[0106] Taking the application to the flow cell slide 1 shown in Figures 1A to 1C as an example, the working principle of the reagent loading system of the present application is described. Among them, the processor 43 controls the on-off of each first valve 41 and the second valve 42, so as to achieve the connectivity between the fluid storage module 101, the fluid loading module 100, the flow cell slide 1 and the waste liquid collection module 102, and then guide the flow direction of the fluid. Therefore, the fluid loading module 100 can be switched to different working modes, and the switching method is highly flexible. In the present embodiment, the fluid loading module 100 can switch between the first working mode to the eleventh working mode. The arrows in Figures 7A to 7K show the flow direction of the fluid under each working mode.

[0107] First working mode:

[0108] Referring to Figure 7A , the second valve 42 is controlled to open to connect the first section 20a1 and the second section 20a2 of the first flow channel 20a, while all first valves 41 are closed. Thus, under the action of the first power source 101c, the first fluid (e.g., a reagent) within the first fluid source 101a can enter the first section 20a1 of the first flow channel 20a through at least one first fluid inlet 21 and / or the third fluid inlet 23, then enter the second section 20a2 of the first flow channel 20a through the second valve 42, then exit the manifold block 20 through the first fluid outlet 24, and finally enter the first waste device 102a. Similarly, under the action of the first power source 101c, the second fluid (e.g., a cleaning solution) within the second fluid source 101b can enter the first section 20a1 of the first flow channel 20a through the third fluid inlet 23, then enter the second section 20a2 of the first flow channel 20a through the second valve 42, then exit the manifold block 20 through the first fluid outlet 24, and finally enter the first waste device 102a.

[0109] In the first working mode, the first fluid in the first fluid source 101a or the second fluid in the second fluid source 101b is guided to be discharged directly to the first waste liquid device 102a without passing through the flow cell slide 1, thereby achieving fluid guidance and discharge.

[0110] Second working mode:

[0111] 7B , the first valve 41 connected to the second opening 1b is controlled to open, while the second valve 42 and the other first valves 41 are closed. Thus, under the action of the first power source 101c, the first fluid (e.g., a reagent) in the first fluid source 101a can enter the second section 20a2 of the first flow channel 20a through the second fluid inlet 22 and the first valve 41 connected to the second opening 1b, and then exit the manifold block 20 through the first fluid outlet 24 and finally enter the first waste device 102a.

[0112] In the second working mode, the first fluid in the first fluid source 101a is guided to be discharged directly to the first waste liquid device 102a without passing through the flow cell slide 1, thereby achieving the guidance and discharge of the fluid.

[0113] The third working mode:

[0114] Referring to Figure 7C , with the first opening 1a serving as the inlet of the flow cell slide 1, the entire flow channel within the flow cell slide 1 is evenly spread with liquid, thereby achieving incubation or cleaning of the flow cell slide 1. Specifically, the first valve 41 connected to the first opening 1a is controlled to open, and the first valves 41 connected to the second opening 1b, the third opening 1c, and / or the fourth opening 1d are controlled to open. Simultaneously, the second valve 42 is closed to disconnect the first section 20a1 and the second section 20a2 of the first flow channel 20a. In this manner, the first fluid (e.g., a reagent) within the first fluid source 101a can enter the first section 20a1 of the first flow channel 20a through at least one first fluid inlet 21 and / or the third fluid inlet 23 under the action of the power source 101c, then enter the flow cell slide 1 through the first valve 41 connected to the first opening 1a, and then enter the second section 20a2 of the first flow channel 20a through other openings of the flow cell slide 1. Finally, the fluid is discharged from the manifold block 20 through the first fluid outlet 24, and finally enters the first waste liquid device 102a. Under the action of the first power source 101c, the second fluid (e.g., cleaning fluid) in the second fluid source 101b can also enter the first section 20a1 of the first flow channel 20a through the third fluid inlet 23, then enter the flow cell slide 1 through the first valve 41 connected to the first opening 1a, and then enter the second section 20a2 of the first flow channel 20a through other openings of the flow cell slide 1. It then exits the manifold block 20 through the first fluid outlet 24 and finally enters the first waste device 102a. The figure shows the fluid flow direction when all first valves 41 connected to the four openings are open.

[0115] In the third working mode, by guiding the first fluid in the first fluid source 101a or the second fluid in the second fluid source 101b to the flow cell carrier 1, uniform liquid spreading of the entire flow channel in the flow cell carrier 1 is achieved, and the residual fluid after liquid spreading is finally discharged into the first waste liquid device 102a to achieve classified treatment of waste liquid.

[0116] Fourth working mode:

[0117] Referring to Figure 7D , the flow cell slide 1 is cleaned using the second opening 1b as the inlet. Specifically, the first valve 41 connected to the first opening 1a, the third opening 1c, and / or the fourth opening 1d is controlled to open. It should be understood that when the first valve 41 connected to the first opening 1a is opened, the second valve 42 is simultaneously controlled to open to connect the first section 20a1 and the second section 20a2 of the first flow channel 20a. In this way, the first fluid (e.g., a reagent) within the first fluid source 101a can enter the second opening 1b of the flow cell slide 1 directly through the second fluid inlet 22 under the action of the first power source 101c, then enter the first flow channel 20a through the other openings of the flow cell slide 1, and then exit the manifold block 20 through the first fluid outlet 24, finally entering the first waste liquid device 102a.

[0118] In the fourth working mode, the first fluid in the first fluid source 101a is guided to the flow cell slide 1, thereby achieving incubation of the flow cell slide 1, and the waste liquid is finally discharged into the first waste liquid device 102a, thereby achieving classified treatment of the waste liquid. Among them, under the premise that the flow cell slide 1 and the rear end flow channel are filled with fluid, no partition is required between the fluid storage module 101 and the flow cell slide 1, so that the pre-discharge of the fluid can be achieved. Moreover, the first fluid can directly enter the second opening 1b of the flow cell slide 1 without passing through the first valve 41 or other reversing components (such as solenoid valves, rotary valves, etc.), avoiding the situation where the first valve 41 or other reversing components may apply a large shear force to the first fluid, thereby damaging certain sensitive components of the first fluid, thereby reducing the damage to the first fluid by external forces.

[0119] Fifth working mode:

[0120] Referring to Figure 7E , the second valve 42 and all first valves 41 are controlled to open. Thus, under the action of the first power source 101c, the second fluid (e.g., cleaning fluid) in the second fluid source 101b can enter the first segment 20a1 of the first flow channel 20a through the third fluid inlet 23, then flow through the second segment 20a2 of the first flow channel 20a through the second valve 42, and then exit the manifold block 20 through the first fluid outlet 24, ultimately entering the first waste device 102a. Simultaneously, after entering the first segment 20a1 of the first flow channel 20a through the third fluid inlet 23, the second fluid in the second fluid source 101b can also enter the flow cell slide 1 through the first valve 41 connected to the first opening 1a, then enter the second segment 20a2 of the first flow channel 20a through other openings of the flow cell slide 1, then exit the manifold block 20 through the first fluid outlet 24, and ultimately enter the first waste device 102a.

[0121] In the fifth working mode, the entire manifold block 20 is cleaned by guiding the second fluid in the second fluid source 101b to the first flow channel 20a and the flow cell slide 1, and the waste liquid is finally discharged into the first waste liquid device 102a to achieve classified treatment of the waste liquid.

[0122] Sixth working mode:

[0123] Referring to Figure 7F , the flow cell carrier 1 is removed from the carrier mounting platform 30 of the fluid loading module 100, or the flow cell carrier 1 is loosened, resulting in an unsealed connection between the flow cell carrier 1 and the manifold block 20. The first valve 41 connected to the first opening 1a is controlled to open, while the second valve 42 and the other first valves 41 are closed. Thus, under the action of the first power source 101c, the second fluid (e.g., cleaning fluid) in the second fluid source 101b enters the first section 20a1 of the first flow channel 20a through the third fluid inlet 23. It then passes through the first valve 41 connected to the first opening 1a, enters the first hole 28a of the carrier interface 28, and is pushed out of the third hole 29a of the seal 29. It then enters the second flow channel 20b through the fourth hole 29b of the seal 29, exits the manifold block 20 through the second fluid outlet 25, and finally enters the second waste device 102b. Similarly, after opening the second valve 42 , the first valve 41 connected to the second opening 1 b , the third opening 1 c or the fourth opening 1 d can be opened separately to clean the corresponding sealing member 29 .

[0124] In the sixth working mode, the second fluid in the second fluid source 101b is guided to the sealing member 29, thereby cleaning the entire sealing member 29, and the waste liquid is finally discharged into the second waste liquid device 102b, thereby achieving classified treatment of the waste liquid.

[0125] Seventh working mode:

[0126] Referring to Figure 7G , the second valve 42 and all the first valves 41 are controlled to be closed. Thus, the first fluid (e.g., a reagent) in a channel of the first fluid source 101a enters the first section 20a1 of the first flow channel 20a through a first fluid inlet 21 under the action of the first power source 101c, and then returns to another channel of the first fluid source 101a through another first fluid inlet 21, thereby mixing with the different types of first fluid in the channel.

[0127] In the seventh working mode, the first fluid in a certain channel of the first fluid source 101 a is guided back to another channel of the first fluid source 101 a , thereby achieving mixing of different types of first fluids.

[0128] Eighth working mode:

[0129] Referring to Figure 7H , the second valve 42 and all first valves 41 are controlled to be closed. Thus, the second fluid (e.g., cleaning fluid) from the second fluid source 101b, under the action of the first power source 101c, enters the first section 20a1 of the first flow channel 20a through the third fluid inlet 23, and then flows into the first fluid source 101a through the first fluid inlet 21, thereby cleaning the first fluid source 101a.

[0130] In the eighth working mode, the first fluid source 101 a is cleaned by guiding the second fluid of the second fluid source 101 b to the first fluid source 101 a .

[0131] Ninth working mode:

[0132] 7I , the first valve 41 connected to the first opening 1a is controlled to be open, while the second valve 42 and the other first valves 41 are controlled to be closed. Thus, under the action of the first power source 101c, the first fluid (e.g., a reagent) in the first fluid source 101a can enter the first section 20a1 of the first flow channel 20a through at least one first fluid inlet 21, then enter the flow cell slide 1 through the first valve 41 connected to the first opening 1a, then flow out of the flow cell slide 1 through the first valve 41 connected to the second opening 1b, and finally return to the first fluid source 101a through the second fluid inlet 22.

[0133] In the ninth working mode, the first fluid in the first fluid source 101a is guided to the flow cell slide 1, and the first fluid in the flow cell is guided back to the first fluid source 101a.

[0134] Tenth working mode:

[0135] 7J , the first valve 41 connected to the first opening 1a is controlled to be open, while the second valve 42 and other first valves 41 are controlled to be closed. Thus, the first fluid (e.g., a reagent) in the first fluid source 101a can enter the second opening 1b of the flow cell slide 1 through the second fluid inlet 22 under the action of the first power source 101c, then flow out of the flow cell slide 1 through the first valve 41 connected to the first opening 1a, and finally return to the first fluid source 101a through at least one first fluid inlet 21.

[0136] In the tenth working mode, the first fluid in the first fluid source 101a is guided to the flow cell slide 1, and the first fluid in the flow cell is guided back to the first fluid source 101a.

[0137] Eleventh working mode:

[0138] Referring to FIG. 7K , the flow cell slide 1 is removed from the slide mounting platform 30 of the fluid loading module 100, or the flow cell slide 1 is loosened to disengage the flow cell slide 1 from the manifold block 20. The first valve 41 connected to the first opening 1a is controlled to open, while the second valve 42 and other first valves 41 are controlled to close. Thus, under the action of the first power source 101c, the first fluid (e.g., a reagent) in the first fluid source 101a can enter the first section 20a1 of the first flow channel 20a through at least one first fluid inlet 21, then enter the first hole 28a of the slide interface 28 through the first valve 41 connected to the first opening 1a, and be pushed out of the third hole 29a of the seal 29. Then, the fluid enters the second flow channel 20b through the fourth hole 29b of the seal 29, and then exits the manifold block 20 through the second fluid outlet 25, finally entering the second waste device 102b. At this time, the area from the first fluid source 101a to the first opening 1a of the flow cell slide 1 (ie, upstream of the first opening 1a) is filled with the high-purity first fluid.

[0139] Then, the flow cell slide 1 is reinstalled or pressed onto the slide mounting platform 30, so that the opening of the flow cell slide 1 is aligned one by one with the seal 29 and the seal 29 is pressed. At this time, the third hole 29a of the seal 29 is connected to the opening of the flow cell slide 1, and the third hole 29a and the fourth hole 29b of the seal 29 are isolated from each other. At this time, the first opening 1a is used as the entrance of the flow cell slide 1, and the entire flow channel in the flow cell slide 1 is evenly spread with liquid, thereby achieving incubation or cleaning of the flow cell slide 1 (refer to the third working mode). Since the upstream of the first opening 1a is filled with a high-purity first fluid, the high-purity first fluid can be guided into the flow cell.

[0140] In the eleventh operating mode, the flow cell slide 1 and the manifold block 20 are first connected in an unsealed state, the desired first fluid is introduced into the first opening 1a of the flow cell slide 1, and then the flow cell slide 1 is reinstalled or compressed to directly introduce the high-purity first fluid into the flow cell slide 1, thereby achieving liquid splatting of the flow cell slide 1. From the beginning to the end of the liquid splatting process, the fluid entering the flow cell slide 1 is always a high-purity fluid of the same composition.

[0141] Referring to FIG8 , one embodiment of the present application further provides a method for fluid loading, which can be applied to the above-mentioned fluid loading module 100 or fluid loading system 200 . The order of the steps in the above-mentioned method can be changed according to different requirements, and some steps can be omitted or combined. The above-mentioned fluid loading method includes the following steps:

[0142] Step S1 : Install the flow cell slide 1 on the manifold block 20 . The flow cell slide 1 includes a plurality of openings, and the first hole 28 a of each slide interface 28 is sealed and docked with an opening of the flow cell slide 1 .

[0143] Step S2: connecting the first fluid inlet 21 and the second fluid inlet 22 to a first fluid source 101 a , wherein the first fluid source 101 a stores at least one first fluid.

[0144] In step S3, the first valve 41 corresponding to the first fluid inlet 21 is controlled to be open, and the first fluid is introduced into the connected slide interface 28 through the first fluid inlet 21, so that the first fluid enters an opening (for example, in the third working mode, the first fluid enters the first opening 1a).

[0145] The first power source 101 c connected to the first fluid source 101 a can operate accordingly, thereby providing power for the first fluid to enter the first fluid inlet 21 .

[0146] In step S4, the first fluid is introduced into the connected slide interface 28 through the second fluid inlet 22, so that the first fluid enters another opening (for example, in the fourth working mode, the first fluid enters the second opening 1b).

[0147] The first power source 101 c connected to the first fluid source 101 a can operate accordingly, thereby providing power for the first fluid to enter the second fluid inlet 22 .

[0148] It will be appreciated that the fluid loading method of the present application is not limited to the steps described above. By controlling the valves and / or the first power source 101c and the second power source 102c, other steps may be executed to achieve different functions of the fluid loading module 100. For details, please refer to the various operating modes listed in Figures 7A to 7K , and a detailed description thereof will not be repeated here.

[0149] The fluid loading system 200 provided in the present application can be used in practical applications in some links in the field of molecular diagnosis or in vitro diagnosis that require liquid spreading and cleaning. For example, it can be applied to nucleic acid sequencing to evenly spread nucleic acid samples or reagents in the flow cell carrier 1. After the liquid spreading is completed, it is used to clean the pipes and seals 29 of the fluid loading module 100, and also to clean the flow channels and openings of the flow cell carrier 1. Referring to Figure 9, the present application also provides a gene sequencing device 300, which includes the fluid loading system 200 or the fluid loading module 100. Through efficient fluid loading, fluid replacement and avoiding cross-contamination between different fluids, the consumption of fluid in the high-throughput sequencing process can be reduced, the volume of the gene sequencing device 300 can also be miniaturized, and the sequencing speed and sequencing cost can be optimized.

[0150] The fluid loading module 100, fluid loading system 200, fluid loading method and gene sequencing device 300 provided by the present application can realize fluid loading of a flow cell slide 1 of large size and small aspect ratio. At least one slide interface 28 of the manifold block 20 can be used not only as an inlet but also as an outlet (that is, at least one opening of the flow cell slide 1 can be used not only as an inlet but also as an outlet), and then through the control of the valve device 40, the loading direction and loading sequence of the fluid are flexible, thereby realizing efficient fluid loading and fluid replacement and avoiding cross contamination between different fluids. Since the present application can realize efficient fluid replacement and avoid cross contamination between different fluids, it can also reduce the consumption of fluid, the volume of the gene sequencing device 300 can be miniaturized, and the sequencing speed and sequencing cost can be optimized.

[0151] Furthermore, the fluid in the fluid storage module 101 can enter the waste liquid collection module 102 or re-enter the fluid storage module 101 via the flow cell carrier 1. The fluid in the fluid storage module 101 can also directly enter the waste liquid collection module 102 or the fluid storage module 101 without passing through the flow cell carrier 1, thereby realizing the classification processing of the fluid (for example, the collection that can be directly discarded directly enters the waste liquid collection module 102, while the reagent that needs to be reprocessed re-enters the fluid storage module 101).

[0152] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A fluid loading module for loading fluid onto a solid-phase slide, the solid-phase slide including a plurality of openings, characterized in that, The fluid loading module includes: a manifold block, in which a first flow channel is provided. The manifold block is provided with a first fluid inlet, a second fluid inlet, a first flow channel outlet, and a plurality of carrier interfaces. The first fluid inlet and the second fluid inlet are both used to communicate with a first fluid source. The first fluid inlet and the first flow channel outlet are both connected to the first flow channel. Each carrier interface is provided with a first hole, and the first hole of each carrier interface is used to dock with an opening of the solid-phase carrier; and a valve device, including a plurality of first valves. Each first valve is connected to the first flow channel and the first hole of a carrier interface. Each first valve is used to connect or disconnect the first flow channel and the first hole, so that the first fluid inlet can communicate with the corresponding carrier interface through a first valve, and the second fluid inlet communicates with another carrier interface.

2. The fluid loading module according to claim 1, characterized in that, The fluid path between the first fluid inlet and the connected carrier interface does not overlap with the fluid path between the second fluid inlet and the connected carrier interface.

3. The fluid loading module according to claim 2, characterized in that, The first fluid inlet and the second fluid inlet are used to communicate with different channels of the first fluid source.

4. The fluid loading module according to claim 2, characterized in that, The fluid path between the second fluid inlet and the connected carrier interface does not pass through the first valve.

5. The fluid loading module according to claim 1, characterized in that, There are a plurality of first fluid inlets, and the plurality of first fluid inlets are used to communicate with different channels of the first fluid source.

6. The fluid loading module according to claim 5, characterized in that, The valve device further includes a second valve, which is connected to the first flow channel and is used to connect or disconnect the first flow channel. The first flow channel includes a first section and a second section on both sides of the second valve, and a plurality of the first fluid inlets are all connected to the first section.

7. The fluid loading module according to claim 1, characterized in that, The manifold block is further provided with a third fluid inlet, which is connected to the first flow channel, and the third fluid inlet is used to communicate with a second fluid source.

8. The fluid loading module according to claim 1, characterized in that, The fluid loading module further includes a seal provided in the carrier interface. The seal is provided with a third hole, and the third hole is used to dock with the first hole and the opening.

9. The fluid loading module according to claim 8, characterized in that, A second flow channel is further provided in the manifold block. The manifold block is provided with a second fluid outlet communicating with the second flow channel. Each carrier interface is further provided with a second hole, and the second hole is used to communicate with the second flow channel. The seal is further provided with a fourth hole, and the fourth hole is docked with the second hole. The fourth hole allows the fluid flowing out of the third hole to enter the second hole.

10. A fluid loading system, characterized in that, including: the fluid loading module according to any one of claims 1 to 9; and a first fluid source, which is used to store at least one first fluid, and the first fluid source communicates with the first fluid inlet and the second fluid inlet in the fluid loading module.

11. The fluid loading system according to claim 10, characterized in that, The first fluid source has a plurality of channels to store different types of the first fluid, and the first fluid inlet and the second fluid inlet communicate with different channels respectively.

12. The fluid loading system according to claim 10, characterized in that, The fluid loading system further includes: a first temperature control module, which is used to provide a temperature for the first fluid in the solid carrier.

13. The fluid loading system according to claim 10, characterized in that, The fluid loading system further includes: a second temperature control module, which is used to provide a temperature for the first fluid in the first fluid source.

14. A gene sequencing device, characterized in that, Comprising the fluid loading module as described in any one of claims 1 to 9, or comprising the fluid loading system as described in any one of claims 10 to 13.

15. A fluid loading method applied to the fluid loading module according to claim 1, characterized in that, The fluid loading method comprises: Mounting the solid-phase carrier on the manifold block, the solid-phase carrier comprising a plurality of openings, and sealingly docking the first hole of each carrier interface with one of the openings of the solid-phase carrier; Connecting the first fluid inlet and the second fluid inlet to a first fluid source respectively, the first fluid source storing at least one first fluid; Controlling the first valve corresponding to the first fluid inlet to be turned on, and introducing the first fluid into the connected carrier interface through the first fluid inlet, so that the first fluid enters one of the openings and flows out from at least one other opening; and Introducing the first fluid into the connected carrier interface through the second fluid inlet, so that the first fluid enters another one of the openings and flows out from at least one other opening.

16. The fluid loading method according to claim 15, wherein, The first fluid inlet and the second fluid inlet are respectively connected to different channels of the first fluid source, and the first fluid introduced through the first fluid inlet is different from the first fluid introduced through the second fluid inlet.

17. The fluid loading method according to claim 16, wherein, The fluid path between the first fluid inlet and the connected carrier interface does not overlap with the fluid path between the second fluid inlet and the connected carrier interface, and the fluid path between the second fluid inlet and the connected carrier interface does not pass through the first valve.

18. The fluid loading method according to claim 15, wherein, There are a plurality of first fluid inlets which are respectively connected to different channels of the first fluid source, and the fluid loading method further comprises: Introducing the first fluid from one of the channels of the first fluid source into the first flow channel through one of the first fluid inlets, so that the first fluid enters another channel of the first fluid source through another one of the first fluid inlets.

19. The fluid loading method according to claim 15, wherein, The fluid loading method further comprises: Controlling the first valve corresponding to the second fluid inlet to be turned on, and introducing the first fluid into the connected carrier interface through the second fluid inlet, so that the first fluid flows to the first fluid outlet after passing through the first valve.

20. The fluid loading method according to claim 15, wherein, The valve device further comprises a second valve connected to the first flow channel, and the fluid loading method further comprises: Controlling the second valve to be turned on; and Introducing the first fluid into the first flow channel through the first fluid inlet, so that the first fluid flows to the first fluid outlet through the second valve.

21. The fluid loading method according to claim 20, wherein, A third fluid inlet is further formed in the manifold block, the third fluid inlet being connected to the first flow channel, and the fluid loading method further comprises: Connecting the third fluid inlet to a second fluid source, the second fluid source storing a second fluid; and Controlling the second valve and each of the first valves to be turned on, and introducing the second fluid into the first flow channel through the third fluid inlet, so that the second fluid enters the solid-phase carrier through the carrier interface connected to at least one of the first valves, or the second fluid flows to the first fluid outlet through the second valve.

22. The fluid loading method according to claim 15, wherein, The manifold block further has a second flow channel, and a second fluid outlet communicating with the second flow channel is defined in the manifold block. Each of the wafer interfaces further has a second hole, and the second hole communicates with the second flow channel. The fluid loading module further includes a seal disposed in the wafer interface. The seal has a third hole docked with the first hole and a fourth hole docked with the second hole. When the solid-phase wafer is installed, the third hole is further docked with the opening.

23. The fluid loading method according to claim 22, wherein, The manifold block further has a third fluid inlet, and the third fluid inlet is connected to the first flow channel. The fluid loading method further includes: Connecting the third fluid inlet to a second fluid source storing a second fluid; Detaching the solid-phase wafer from the manifold block or switching the wafer interface to a non-sealed connection state; and Controlling the first valve corresponding to the first fluid inlet to be turned on, and introducing the second fluid into the first flow channel through the third fluid inlet, so that the second fluid sequentially enters the third hole and the fourth hole through the wafer interface communicated with at least one of the first valves, then enters the second flow channel and flows to the second fluid outlet.

24. The fluid loading method according to claim 22, wherein, The fluid loading method further includes: Detaching the solid-phase wafer from the manifold block or switching the solid-phase wafer to a non-sealed connection state with the manifold block; Controlling the first valve corresponding to the first fluid inlet to be turned on, and introducing the first fluid into the wafer interface communicated with the first fluid inlet, so that the first fluid sequentially enters the third hole and the fourth hole through the wafer interface communicated with the first valve, then enters the second flow channel and flows to the second fluid outlet; Reinstalling the solid-phase wafer to the manifold block and switching the manifold block to a sealed connection state; and Continuing to introduce the first fluid into the wafer interface communicated with the first fluid inlet through the first fluid inlet, so that the first fluid enters one of the openings.

25. The fluid loading method according to claim 15, wherein, The manifold block further has a third fluid inlet, and the third fluid inlet is connected to the first flow channel. The fluid loading method further includes: Connecting the third fluid inlet to a second fluid source storing a second fluid; and Introducing the second fluid into the first flow channel through the third fluid inlet, so that the second fluid enters the first fluid source through the first fluid inlet.

26. The fluid loading method according to claim 15, wherein, The first fluid introduced into the wafer interface communicated with the first fluid inlet further flows to the first fluid source through the second fluid interface.

27. The fluid loading method according to claim 15, wherein The first fluid introduced into the wafer interface communicated with the second fluid inlet further flows to the first fluid source through the first fluid interface.

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