Methods and compositions for donor DNA molecules

By using circular single-stranded DNA (cssDNA) donor molecules to form a double-stranded DNA region with substrate complementary oligonucleotides (SCO), and then combining with endogenous DNA polymerase for second-strand synthesis, the risks of immune sensing and integration of dsDNA donor molecules in the cell nucleus are resolved, enabling safe and efficient genome insertion.

CN122374459APending Publication Date: 2026-07-10THE GENERAL HOSPITAL CORP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE GENERAL HOSPITAL CORP
Filing Date
2024-10-11
Publication Date
2026-07-10

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Abstract

We describe compositions of DNA donor molecules compatible with various gene insertion methods, offering key advantages in delivery, nuclear localization, and immune evasion. The modified DNA donor molecules are predominantly single-stranded but include short regions of double-stranded DNA to reconstruct enzyme binding sites, and can be combined with other methods to enhance donor recruitment to the cell nucleus.
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