An electrostatic driving-based double-enzyme cascade amplification fluorescence sensor, a preparation method and application thereof
By combining Azo-Bodipy 685, CHO, and ACh using electrostatic co-assembly technology, a dual-enzyme cascade amplified fluorescence sensor with a nanoscale reaction microenvironment is formed. This solves the diffusion limitation and steric hindrance effect problems of fluorescence sensors when detecting organophosphorus pesticide residues, and achieves rapid, simple, and highly sensitive detection results.
CN122409595APending Publication Date: 2026-07-17SHENZHEN INSTITUTE FOR DRUG CONTROL (SHENZHEN TESTING CENTER OF MEDICAL DEVICES)
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHENZHEN INSTITUTE FOR DRUG CONTROL (SHENZHEN TESTING CENTER OF MEDICAL DEVICES)
- Filing Date
- 2025-11-14
- Publication Date
- 2026-07-17
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Figure CN122409595A_ABST
Abstract
本发明公开了一种基于静电驱动的双酶级联放大荧光传感器及其制备方法和应用，所述纳米酶荧光传感器由探针Azo‑Bodipy 685、胆碱氧化酶（CHO）和氯化乙酰胆碱（ACh）组成。本发明基于静电吸附原理，提出静电络合共固定化‑酶级联反应协同策略，构建一种新的静电驱动荧光传感器，利用ACh的季铵盐正电荷与CHO表面负电荷的静电作用，在特异性荧光探针Azo‑Bodipy 685表面构建纳米尺度空间限域的酶反应微环境。当有机磷农药抑制乙酰胆碱酯酶（AChE）时，氯化胆碱生成减少导致CHO催化产生的过氧化氢量下降，荧光信号强度减弱，一系列荧光强度的变化可以实现检测AChE活性和测定有机磷农药残留含量。
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