Highly sensitive raman probe for cadmium ion based on nucleic acid aptamer modification
By employing a cadmium ion Raman probe modified with nucleic acid aptamers and utilizing a dual covalent binding mechanism of thiol-gold bonds and amide bonds, high sensitivity and specificity of cadmium ion detection are achieved. This solves the problems of complex operation, insufficient sensitivity, and susceptibility to interference in existing technologies, and is suitable for rapid detection of environmental water samples, food samples, and biological fluids.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUIZHOU ACADEMY OF TESTING & ANALYSIS
- Filing Date
- 2025-12-03
- Publication Date
- 2026-07-17
AI Technical Summary
Existing cadmium ion detection technologies are complex to operate, have poor specificity and insufficient sensitivity, cannot achieve accurate detection of low concentrations of cadmium ions, and are easily affected by interference from coexisting ions.
A highly sensitive cadmium ion Raman probe based on nucleic acid aptamer modification is used. It is stably bound to noble metal nanoparticles through thiol-gold bonds and covalently coupled to Raman reporter molecules through amide bonds, thereby achieving specific recognition and conformational change of cadmium ions and triggering changes in Raman signal intensity.
It achieves highly sensitive detection of cadmium ions with a detection limit as low as 0.05 nM, high specificity, minimal interference from coexisting ions, good stability, adaptability to different pH environments, and suitability for rapid on-site detection.
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Figure CN122409613A_ABST