Process for the production of high-nutritional double-protein pet food

By employing targeted fermentation enzymatic hydrolysis, isoelectric point co-precipitation, and microencapsulation technologies, combined with a seven-stage twin-screw high-moisture extrusion molding and low-temperature drying process, the problems of removing anti-nutritional factors, amino acid imbalance, and texture in plant protein pet foods have been solved, achieving the production of pet foods with high nutrient retention and excellent texture.

CN122460604APending Publication Date: 2026-07-28HAINAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HAINAN UNIV
Filing Date
2026-05-11
Publication Date
2026-07-28

AI Technical Summary

Technical Problem

Existing plant-based protein pet foods have significant shortcomings in terms of anti-nutritional factor removal, amino acid composition imbalance, nutrient protection, and texture, resulting in low protein digestibility, high nutrient loss, and poor texture, making it difficult to meet the nutritional needs of pets, especially cats.

Method used

Plant protein raw materials are processed using directional fermentation and enzymatic hydrolysis technology. Dual proteins are prepared by isoelectric point co-precipitation. Microcapsules are then encapsulated using a zein-pectin-liposome composite wall material. Combined with a seven-segment twin-screw high-moisture extrusion molding and low-temperature drying process, a meat-like fiber structure is formed. Heat-sensitive nutrient microcapsules are then sprayed on to achieve efficient amino acid complementarity and nutrient retention.

Benefits of technology

It significantly improved protein digestibility, amino acid complementarity, nutrient retention and texture properties, with protein digestibility increasing to 93.2%, PDCAAS reaching 0.98, nutrient retention reaching 90.1%, and texture recovery rate increasing to 89%.

✦ Generated by Eureka AI based on patent content.

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Abstract

This invention relates to the field of pet food processing technology, specifically a processing method for high-nutrient dual-protein pet food, including: raw material pretreatment: processing plant protein raw materials using directional fermentation enzymatic hydrolysis technology to obtain a protein solution; isoelectric point co-precipitation to prepare dual proteins: adjusting the pH of the protein solution to the isoelectric point, and centrifuging to obtain a dual-protein complex; microencapsulation treatment: using a zein-pectin-liposome composite wall material to microencapsulate fat-soluble nutrients to obtain microencapsulated nutrients; seven-segment twin-screw high-moisture extrusion molding: mixing the dual-protein complex, microencapsulated nutrients, and other ingredients, and extruding under high moisture conditions using a seven-segment twin-screw extruder to obtain extruded material; microcapsule spraying reinforcement, drying and freshness locking, and packaging. This invention achieves a comprehensive improvement in protein quality, nutrient retention rate, texture, palatability, and digestibility in pet food.
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Description

Technical Field

[0001] This invention relates to the field of pet food processing technology, specifically a processing method for high-nutrition, dual-protein pet food. Background Technology

[0002] In recent years, with the accelerating trend of pet ownership in households, the pet food market has shown a clear trend of consumption upgrading. Plant-based pet food has gained increasing market attention due to its advantages such as being environmentally friendly and sustainable, and avoiding meat allergens. However, compared with the rapidly developing market demand, existing plant protein pet foods still have significant shortcomings in key technological aspects, which seriously restricts further improvement in product quality and market acceptance.

[0003] In terms of raw material pretreatment, the anti-nutritional factors commonly found in plant proteins are the primary technical challenge. Phytic acid and trypsin inhibitors, present in commonly used plant proteins such as soybeans and wheat, can chelate with minerals, directly affecting protein digestibility and absorption. Traditional removal methods often employ high-temperature heat treatment or strong acid / alkali chemical treatment. These methods not only lead to excessive protein denaturation and decreased functional properties but may also produce unpleasant flavors and pose a risk of chemical residues, which does not align with current consumer trends emphasizing clean labeling.

[0004] In terms of nutritional composition, plant proteins generally suffer from an imbalance in amino acid composition. Soy protein lacks sulfur-containing amino acids, and wheat protein is severely deficient in lysine, making it difficult to meet the nutritional needs of pets, especially carnivores such as cats. Traditional physical mixing methods cannot achieve amino acid complementarity at the molecular level, resulting in generally low protein digestibility-corrected amino acid scores (PDCAAS), ranging only from 0.6 to 0.7, far below the 1.0 standard for animal proteins, severely impacting protein utilization efficiency.

[0005] Regarding nutrient protection during processing, fat-soluble vitamins and polyunsaturated fatty acids such as taurine are highly sensitive to conditions such as light, heat, and oxygen. Conventional processing methods can cause significant losses of these heat-sensitive nutrients, with a loss rate exceeding 40%. Existing microencapsulation technologies, due to limitations in wall material selection, typically have an encapsulation efficiency of less than 80%, and these nutrients are easily destroyed in the highly acidic environment of a pet's stomach, leading to a substantial reduction in nutrient bioavailability.

[0006] In terms of product texture, products produced by traditional low-moisture extrusion technology have coarse fiber structures, poor water retention (typically <3 grams of water / gram of dry matter), a hard and brittle texture, and a strong powdery feel. They easily become soft and mushy after rehydration, failing to simulate the juiciness and firmness of real meat. Although high-moisture extrusion technology has been proven to improve texture, precisely controlling process parameters to stably form nanoscale fiber structures and ideal protein networks remains a technical challenge for the industry.

[0007] Based on this, a processing method for high-nutrition dual-protein pet food is proposed. Summary of the Invention

[0008] To address the shortcomings of existing technologies, this invention provides a processing method for high-nutrition dual-protein pet food, achieving a comprehensive improvement in protein quality, nutrient retention rate, texture, palatability, and digestibility.

[0009] To achieve the above objectives, the present invention provides the following technical solution: a processing method for high-nutrition dual-protein pet food, comprising the following steps: (1) Raw material pretreatment: Plant protein raw materials are treated by directional fermentation enzymatic hydrolysis technology to degrade phytic acid and antigenic proteins, generate small molecule active substances, and obtain protein liquid; (2) Preparation of dual proteins by isoelectric point coprecipitation: Adjust the pH of the protein solution to the isoelectric point, and centrifuge after standing to obtain the dual protein complex; (3) Microencapsulation treatment: Fat-soluble nutrients were microencapsulated using a zein-pectin-liposome composite wall material with an encapsulation efficiency of ≥95.8% to obtain microencapsulated nutrients; (4) Seven-segment twin-screw high-moisture extrusion molding: The dual protein complex, microencapsulated nutrients and other ingredients are mixed and extruded under high moisture conditions by a seven-segment twin-screw extruder to form a meat-like fiber structure and obtain the extruded material. (5) Microcapsule spraying reinforcement: Spray a microcapsule suspension containing heat-sensitive nutrients onto the surface of the extruded material to further improve the nutrient retention rate; (6) Drying and packaging: Low-temperature drying process is used to remove excess moisture and then packaged to obtain the finished product.

[0010] Preferably, in step (1), the plant protein raw material is a mixture of soybean protein and wheat protein, and the mass ratio of soybean protein to wheat protein is (1-3):1.

[0011] Preferably, in step (1), the plant protein raw material is a mixture of soybean protein and pea protein, and the mass ratio of soybean protein to pea protein is (1-2):1.

[0012] Preferably, in step (1), the directional fermentation enzymatic hydrolysis technology is carried out at 40-50℃ for 3-5 hours.

[0013] Preferably, in step (2), the isoelectric point pH value is 4.3-4.7, and the protein concentration of the dual protein complex is controlled to be 15-25%.

[0014] Preferably, in step (3), the mass ratio of zein, pectin and liposomes is 3:1:1; the fat-soluble nutrients include taurine, vitamin A and vitamin D.

[0015] Preferably, in step (4), the total moisture content of the material under high moisture conditions is 55-65%, the temperature ranges of the first to seventh zones of the seven-segment twin-screw extruder are 75-85℃, 95-105℃, 115-125℃, 125-135℃, 120-130℃, 110-120℃, and 90-100℃, respectively, and the screw speed is 180-220 rpm.

[0016] Preferably, in step (6), the drying temperature of the low-temperature drying process is 55-65℃, and the moisture content of the finished product after drying is ≤10%.

[0017] This invention provides a processing method for high-nutrition dual-protein pet food, which has the following advantages compared with the prior art: 1. This invention employs directional fermentation enzymatic hydrolysis pretreatment, leaving no chemical residues, and increases protein digestibility from the traditional 70.3% to a maximum of 93.2%; 2. This invention utilizes isoelectric point coprecipitation to achieve molecular-level amino acid complementarity, achieving a PDCAAS of 0.98, which is more than 30% higher than that of traditional plant proteins; 3. The microcapsule encapsulation efficiency of this invention reaches up to 96.2%, and the nutrient retention rate reaches 90.1%; 4. This invention utilizes high-moisture extrusion to form a meat-like fiber structure, increasing water retention by 60% and texture recovery rate from 65% to a maximum of 89%; 5. The overall process of this invention is highly efficient and controllable, and it combines high nutrition with high palatability. Attached Figure Description

[0018] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 This is a process flow diagram of Embodiment 2 of the present invention; Figure 2 This is a process equipment flow chart in Embodiment 2 of the present invention. Detailed Implementation

[0019] The following embodiments are provided to illustrate the implementation of this application in detail, so that the process of how this application uses technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0020] Example 1 The processing method for high-nutrient dual-protein pet food includes the following steps: (1) Raw material pretreatment: Soy protein and wheat protein were mixed at a mass ratio of 2:1 and enzymatic hydrolysis was carried out using directional fermentation (composed of Bacillus licheniformis alkaline protease and Aspergillus oryzae flavor protease at a mass ratio of 2:1, with the amount accounting for 2% of the total weight of the substrate protein). The mixture was enzymatically hydrolyzed at 45℃ for 4 hours to degrade phytic acid and antigen protein, and a protein solution was obtained. (2) Preparation of dual proteins by isoelectric point coprecipitation: The pH of the protein solution was adjusted to 4.5 with acid (food grade hydrochloric acid, 1 mol / L), centrifuged and the precipitate was collected to obtain the dual protein complex, and the protein concentration was controlled to be 20%; (3) Microencapsulation treatment: The fat-soluble nutrients: taurine, vitamin A and vitamin D mixture (0.1g: 5000IU: 200IU) were microencapsulated using a zein-pectin-liposome composite wall material (3:1:1, w / w / w). The encapsulation efficiency was 96.2%. The fat-soluble nutrients were mixed with the composite wall material (1:6, w / w) to obtain microencapsulated nutrients. (4) Seven-stage twin-screw high-moisture extrusion molding: The dual-protein complex, microencapsulated nutrients and other ingredients are mixed in the following mass ratios: dual-protein complex 62.5%, microencapsulated nutrients 1.25%, starch 22.5% (cassava starch: rice flour = 1:1), oil 10% (chicken oil: soybean oil = 3:1), salt and minerals 2%, and other functional additives 1.75% (cellulose powder 1.0%, lecithin 0.5%, rosemary extract 0.25%). The total moisture content of the material is adjusted to 60%, and it is fed into a seven-stage twin-screw extruder. The temperature ranges of zones one to seven are 80℃, 100℃, 120℃, 130℃, 125℃, 115℃, and 95℃, respectively. The screw speed is 220 rpm. High-moisture shear-stretch coupling extrusion is performed to obtain the extruded material. (5) Microcapsule spraying reinforcement: Spray a suspension of heat-sensitive nutrient microcapsules onto the surface of the extruded material; the suspension uses the same corn gliadin-pectin-liposome (3:1:1) composite wall material as in step (3), which encapsulates highly heat-sensitive components such as vitamin C, B vitamins, probiotics, and taurine. Spray evenly at ≤35℃, with a spraying amount of 2.0% of the weight of the extruded material. After spraying, immediately proceed to the low-temperature drying process. (6) Drying and packaging: Dry at 60℃ with low-temperature airflow until the moisture content is ≤10%, and then package after cooling.

[0021] Example 2 The processing method for high-nutrient dual-protein pet food includes the following steps: (1) Raw material pretreatment: Soy protein and wheat protein were mixed at a mass ratio of 1:1 and enzymatic hydrolysis was carried out using directional fermentation (composed of Bacillus licheniformis alkaline protease and Aspergillus oryzae flavor protease at a mass ratio of 2:1, with the amount accounting for 4% of the total weight of the substrate protein). The mixture was enzymatically hydrolyzed at 40℃ for 5 hours to degrade phytic acid and antigenic protein, and protein solution was obtained. (2) Preparation of dual proteins by isoelectric point coprecipitation: The pH of the protein solution was adjusted to 4.3 with acid (food grade hydrochloric acid, 2 mol / L), centrifuged and the precipitate was collected to obtain the dual protein complex, and the protein concentration was controlled to be 15%; (3) Microencapsulation treatment: The fat-soluble nutrients: taurine, vitamin A and vitamin D mixture (0.3g: 10000IU: 400IU) were microencapsulated using a zein-pectin-liposome composite wall material (3:1:1, w / w / w). The encapsulation efficiency was 95.8%. The fat-soluble nutrients were mixed with the composite wall material (1:6, w / w) to obtain microencapsulated nutrients. (4) Seven-stage twin-screw high-moisture extrusion molding: The dual-protein complex, microencapsulated nutrients and other ingredients are mixed in the following mass ratios: dual-protein complex 62.5%, microencapsulated nutrients 1.25%, starch 22.5% (cassava starch: rice flour = 1:1), oil 10% (chicken oil: soybean oil = 3:1), salt and minerals 2%, and other functional additives 1.75% (cellulose powder 1.0%, lecithin 0.5%, rosemary extract 0.25%). The total moisture content of the material is adjusted to 55%, and it is fed into a seven-stage twin-screw extruder. The temperature ranges of zones one to seven are 75℃, 95℃, 115℃, 125℃, 120℃, 110℃ and 90℃, respectively, and the screw speed is 180 rpm. High-moisture shear-stretch coupling extrusion is performed to obtain the extruded material. (5) Microcapsule spraying reinforcement: Spray a suspension of heat-sensitive nutrient microcapsules onto the surface of the extruded material; the suspension uses the same zein-pectin-liposome (3:1:1) composite wall material as in step (3), which encapsulates highly heat-sensitive components such as vitamin C, B vitamins, probiotics, and taurine. Spray evenly at ≤35℃, with a spraying amount of 2.5% of the weight of the extruded material. After spraying, immediately proceed to the low-temperature drying process. (6) Drying and packaging: Dry at 55℃ with low-temperature airflow until the moisture content is ≤10%, and then package after cooling.

[0022] Example 3 The processing method for high-nutrient dual-protein pet food includes the following steps: (1) Raw material pretreatment: Soy protein and pea protein were mixed at a mass ratio of 1:1 and enzymatic hydrolysis was carried out at 50°C for 3 hours to degrade phytic acid and antigenic protein, and protein solution was obtained. The mixture was prepared by directional fermentation enzymatic hydrolysis technology (composed of Bacillus licheniformis alkaline protease and Aspergillus oryzae flavor protease at a mass ratio of 2:1, with the amount accounting for 6.0% of the total weight of substrate protein). (2) Preparation of dual proteins by isoelectric point coprecipitation: The pH of the protein solution was adjusted to 4.6 with acid (food grade hydrochloric acid, 2 mol / L), centrifuged and the precipitate was collected to obtain the dual protein complex, and the protein concentration was controlled to be 25%; (3) Microencapsulation treatment: The fat-soluble nutrients: taurine, vitamin A and vitamin D mixture (0.4g: 20000IU: 800IU) were microencapsulated using a zein-pectin-liposome composite wall material (3:1:1, w / w / w). The encapsulation efficiency was 96.5%. The fat-soluble nutrients were mixed with the composite wall material (1:6, w / w) to obtain microencapsulated nutrients. (4) Seven-stage twin-screw high-moisture extrusion molding: The dual-protein complex, microencapsulated nutrients and other ingredients are mixed in the following mass ratios: dual-protein complex 62.5%, microencapsulated nutrients 1.25%, starch 22.5% (cassava starch: rice flour = 1:1), oil 10% (chicken oil: soybean oil = 3:1), salt and minerals 2%, and other functional additives 1.75% (cellulose powder 1.0%, lecithin 0.5%, rosemary extract 0.25%). The total moisture content of the material is adjusted to 65%, and it is fed into a seven-stage twin-screw extruder. The temperature ranges of zones one to seven are 85℃, 105℃, 125℃, 135℃, 130℃, 120℃ and 100℃, respectively, and the screw speed is 220 rpm. High-moisture shear-stretch coupling extrusion is performed to obtain the extruded material. (5) Microcapsule spraying reinforcement: Spray a suspension of heat-sensitive nutrient microcapsules onto the surface of the extruded material; the suspension uses the same zein-pectin-liposome (3:1:1) composite wall material as in step (3), which encapsulates highly heat-sensitive components such as vitamin C, B vitamins, probiotics, and taurine. Spray evenly at ≤35℃, with a spraying amount of 2.2% of the weight of the extruded material. After spraying, immediately proceed to the low-temperature drying process. (6) Drying and packaging: Dry at 65℃ with low-temperature airflow until the moisture content is ≤10%, and then package after cooling.

[0023] Example 4 The processing method for high-nutrient dual-protein pet food includes the following steps: (1) Raw material pretreatment: Soy protein and wheat protein were mixed at a mass ratio of 3:1 and enzymatic hydrolysis was carried out using directional fermentation (composed of Bacillus licheniformis alkaline protease and Aspergillus oryzae flavor protease at a mass ratio of 2:1, with the amount accounting for 1% of the total weight of the substrate protein). The mixture was enzymatically hydrolyzed at 48℃ for 3.5h to degrade phytic acid and antigenic protein, and a protein solution was obtained. (2) Preparation of dual proteins by isoelectric point coprecipitation: The pH of the protein solution was adjusted to 4.4 with acid (food grade hydrochloric acid, 1 mol / L), centrifuged and the precipitate was collected to obtain the dual protein complex, and the protein concentration was controlled to be 22%; (3) Microencapsulation treatment: The fat-soluble nutrients: taurine, vitamin A and vitamin D mixture (0.2g: 5000IU: 300IU) were microencapsulated using a zein-pectin-liposome composite wall material (3:1:1, w / w / w). The encapsulation efficiency was 96.0%, and microencapsulated nutrients were obtained. (4) Seven-stage twin-screw high-moisture extrusion molding: The dual-protein complex, microencapsulated nutrients and other ingredients are mixed in the following mass ratios: dual-protein complex 62.5%, microencapsulated nutrients 1.25%, starch 22.5% (cassava starch: rice flour = 1:1), oil 10% (chicken oil: soybean oil = 3:1), salt and minerals 2%, and other functional additives 1.75% (cellulose powder 1.0%, lecithin 0.5%, rosemary extract 0.25%). The total moisture content of the material is adjusted to 58%, and it is fed into a seven-stage twin-screw extruder. The temperature ranges of zones one to seven are 78℃, 98℃, 118℃, 128℃, 123℃, 113℃ and 93℃, respectively, and the screw speed is 190 rpm. High-moisture shear-stretch coupling extrusion is performed to obtain the extruded material. (5) Microcapsule spraying reinforcement: Spray a suspension of heat-sensitive nutrient microcapsules onto the surface of the extruded material; the suspension uses the same corn gliadin-pectin-liposome (3:1:1) composite wall material as in step (3), which encapsulates highly heat-sensitive components such as vitamin C, B vitamins, probiotics, and taurine. Spray evenly at ≤35℃, with a spraying amount of 2.0% of the weight of the extruded material. After spraying, immediately proceed to the low-temperature drying process. (6) Drying and packaging: Dry at 58℃ with low-temperature airflow until the moisture content is ≤10%, and then package after cooling.

[0024] Example 5 The processing method for high-nutrient dual-protein pet food includes the following steps: (1) Raw material pretreatment: Soy protein and pea protein were mixed at a mass ratio of 2:1 and enzymatic hydrolysis was carried out at 52℃ for 3 hours to degrade phytic acid and antigenic protein, and protein solution was obtained. (2) Preparation of dual proteins by isoelectric point coprecipitation: The pH of the protein solution was adjusted to 4.7 with acid (food grade hydrochloric acid, 1 mol / L), centrifuged and the precipitate was collected to obtain the dual protein complex, and the protein concentration was controlled to be 18%; (3) Microencapsulation treatment: The fat-soluble nutrients: taurine, vitamin A and vitamin D mixture (0.1g: 5000IU: 200IU) were microencapsulated using a zein-pectin-liposome composite wall material (3:1:1, w / w / w). The encapsulation efficiency was 95.9%. The fat-soluble nutrients were mixed with the composite wall material (1:6, w / w) to obtain microencapsulated nutrients. (4) Seven-stage twin-screw high-moisture extrusion molding: The dual-protein complex, microencapsulated nutrients and other ingredients are mixed in the following mass ratios: dual-protein complex 62.5%, microencapsulated nutrients 1.25%, starch 22.5% (cassava starch: rice flour = 1:1), oil 10% (chicken oil: soybean oil = 3:1), salt and minerals 2%, and other functional additives 1.75% (cellulose powder 1.0%, lecithin 0.5%, rosemary extract 0.25%). The total moisture content of the material is adjusted to 62%, and it is fed into a seven-stage twin-screw extruder. The temperature ranges of zones one to seven are 82℃, 102℃, 122℃, 132℃, 127℃, 117℃ and 97℃, respectively, and the screw speed is 210 rpm. High-moisture shear-stretch coupling extrusion is performed to obtain the extruded material. (5) Microcapsule spraying reinforcement: Spray a suspension of heat-sensitive nutrient microcapsules onto the surface of the extruded material; the suspension uses the same corn gliadin-pectin-liposome (3:1:1) composite wall material as in step (3), which encapsulates highly heat-sensitive components such as vitamin C, B vitamins, probiotics, and taurine. Spray evenly at ≤35℃, with a spraying amount of 2.0% of the weight of the extruded material. After spraying, immediately proceed to the low-temperature drying process. (6) Drying and packaging: Dry at 62℃ with low-temperature airflow until the moisture content is ≤10%, and then package after cooling.

[0025] Performance testing 1. Traditional product preparation methods (1) Raw material mixing: Mix the plant protein powder (22% soy protein isolate, 18% wheat gluten) and starch source (35% corn starch, 25% rice flour) in proportion; (2) Steam conditioning: The mixture is fed into a conditioner and pregelatinized with high-temperature steam. The moisture content of the material is 25%. (3) Extrusion puffing: Extrusion is carried out using a single / twin screw extruder to gelatinize the starch and form a porous structure. The material is extruded, expanded and cut after being subjected to high temperature (160℃) and high pressure (5.0MPa) in the barrel; (4) Drying and cooling: The cut granules are sent into a dryer and the moisture content is reduced to below 10% at 80°C to extend the shelf life; (5) Vacuum spraying: Oils, heat-sensitive vitamins, etc. are adsorbed onto the surface of particles through vacuum spraying process.

[0026] Traditional products do not incorporate conventional production processes that employ new technologies such as "directional fermentation," "isoelectric point co-precipitation," and "microencapsulation."

[0027] 2. The products in Examples 1-5 and the traditional products were tested, and the specific test results are shown in Table 1. The test standards are shown in Table 2.

[0028] Table 1 Performance Comparison

[0029] Table 2 Test Standards

[0030] As can be seen from Table 1, the dual-protein pet food prepared by this invention is significantly superior to traditional processes in terms of key nutritional indicators and physical texture, and can meet the needs of pets for high-quality food.

[0031] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A processing method for high-nutrition, dual-protein pet food, characterized in that, Includes the following steps, (1) Raw material pretreatment: Plant protein raw materials are treated by directional fermentation enzymatic hydrolysis technology to degrade phytic acid and antigenic proteins, generate small molecule active substances, and obtain protein liquid; (2) Preparation of dual proteins by isoelectric point coprecipitation: Adjust the pH of the protein solution to the isoelectric point, and centrifuge after standing to obtain the dual protein complex; (3) Microencapsulation treatment: Fat-soluble nutrients were microencapsulated using a zein-pectin-liposome composite wall material to obtain microencapsulated nutrients; (4) Seven-segment twin-screw high-moisture extrusion molding: The dual protein complex, microencapsulated nutrients and other ingredients are mixed and extruded under high moisture conditions by a seven-segment twin-screw extruder to obtain the extruded material; (5) Microcapsule spraying reinforcement: Spraying a microcapsule suspension containing heat-sensitive nutrients onto the surface of the extruded material; (6) Drying and packaging: Low-temperature drying process is used to remove excess moisture and then packaged to obtain the finished product.

2. The processing method of the high-nutrition dual-protein pet food according to claim 1, characterized in that, In step (1), the plant protein raw material is a mixture of soybean protein and wheat protein, and the mass ratio of soybean protein to wheat protein is (1-3):

1.

3. The processing method of the high-nutrition dual-protein pet food according to claim 2, characterized in that, In step (1), the plant protein raw material is a mixture of soybean protein and pea protein, and the mass ratio of soybean protein to pea protein is (1-2):

1.

4. The processing method of the high-nutrition dual-protein pet food according to claim 1, characterized in that, In step (1), the directional fermentation enzymatic hydrolysis technology is carried out at 40-50℃ for 3-5 hours.

5. The processing method of the high-nutrition dual-protein pet food according to claim 2 or 3, characterized in that, In step (2), the isoelectric point pH value is 4.3-4.7, and the protein concentration of the dual protein complex is controlled at 15-25%.

6. The processing method of the high-nutrition dual-protein pet food according to claim 1, characterized in that, In step (3), the mass ratio of zein, pectin and liposomes is 3:1:1; the fat-soluble nutrients include taurine, vitamin A and vitamin D.

7. The processing method of the high-nutrition dual-protein pet food according to claim 1, characterized in that, In step (4), the total moisture content of the material under high moisture conditions is 55-65%, the temperature ranges of the first to seventh zones of the seven-segment twin-screw extruder are 75-85℃, 95-105℃, 115-125℃, 125-135℃, 120-130℃, 110-120℃, and 90-100℃, respectively, and the screw speed is 180-220 rpm.

8. The processing method of the high-nutrition dual-protein pet food according to claim 1, characterized in that, In step (6), the drying temperature of the low-temperature drying process is 55-65℃, and the moisture content of the finished product after drying is ≤10%.