Compound traditional Chinese medicine preparation for improving musk yield and quality, and preparation and application method thereof

Abstract

This invention discloses a compound traditional Chinese medicine preparation for improving musk yield and quality, as well as its preparation and application methods. It relates to the field of traditional Chinese medicine preparation technology. The compound traditional Chinese medicine preparation, by weight, comprises: 16 parts Epimedium, 7 parts Cuscuta, 11 parts Morinda officinalis, 7 parts Astragalus membranaceus, 14 parts Rehmannia glutinosa, 9 parts Angelica sinensis, 7 parts Codonopsis pilosula, and 4 parts Glycyrrhiza uralensis. Starting 30 days before the musk-secreting season each year, the compound traditional Chinese medicine preparation is mixed into a mixture of succulent and concentrated feed and fed to each male musk deer at a dose of 2g or 4g per day. Results show that feeding 2g of the compound traditional Chinese medicine preparation increases musk yield by 11.20% and muscone content by 48.78%, while feeding 4g increases musk yield by 23.85%. This invention solves the problems of low musk yield, unstable quality, and side effects associated with current techniques, and has the advantages of simple preparation, low cost, and safety and effectiveness.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine preparation technology, and in particular to a compound traditional Chinese medicine preparation for improving the yield and quality of musk, as well as its preparation and application methods. Background Technology

[0002] Musk is a precious traditional Chinese medicine secreted by musk deer. It has the effects of opening the orifices and refreshing the mind, promoting blood circulation and regulating menstruation, reducing swelling and relieving pain. It is the core ingredient of many precious traditional Chinese medicines such as Angong Niuhuang Wan and Musk Heart-Protecting Wan.

[0003] However, musk deer are highly stress-prone animals, exhibiting solitary, territorial, and leaping biological characteristics. Various factors, including the rearing environment, feeding methods, and diet, influence the stress and nutritional physiology of forest musk deer, thereby interfering with their musk secretion process. Currently, the musk yield from captive-bred forest musk deer is significantly lower than that from wild-caught deer, and the quality of musk, indicated by muscone, is also unstable. With the accelerated internationalization of traditional Chinese medicine and the rapid growth in demand for musk-containing Chinese medicinal herbs, the production of captive-bred musk is far from meeting market demand.

[0004] Previous studies have shown that blood and fecal testosterone levels in musk deer are significantly higher during the musk-secreting period than during the non-secreting period, and the male gonads are the physiological organs that initiate musk secretion. Previous studies have attempted to prolong the musk-secreting period and increase the amount of musk secreted by injecting exogenous hormones, but this method caused a strong stress response in the musk deer, negatively impacting their physiological health, and therefore has not been practically adopted.

[0005] Existing traditional Chinese medicine formulas include combinations of ginseng, processed Polygonum multiflorum, Epimedium, and malt, which can restore the musk-secreting function of older male musk deer to some extent. However, they have not improved the physiological activity of male musk deer during their peak musk-secreting period, limiting their application. Another method involves feeding them the flavonoid compound quercetin, which can partially improve musk quality, but has no impact on overall musk production, and the safety of long-term quercetin feeding remains unclear. Furthermore, while simply increasing the amount of high-protein components in the feed, such as soybean meal and mulberry leaves, can increase musk production, excessive protein intake can easily lead to changes in the intestinal flora structure and microecological imbalance in forest musk deer, causing various digestive diseases and even death.

[0006] Therefore, there is an urgent need to develop a safe, effective formulation and application method that can comprehensively improve the properties of musk (including musk yield and quality). Summary of the Invention

[0007] The purpose of this invention is to provide a compound traditional Chinese medicine preparation for improving musk yield and quality, as well as its preparation and application methods. This invention addresses the problems of low musk yield and unstable musk quality in captive forest musk deer, as well as the significant side effects, limited effectiveness, and unclear safety of existing intervention methods. It aims to comprehensively improve the musk performance (including musk yield and quality) of forest musk deer in a safe, economical, and convenient manner.

[0008] To achieve the above objectives, the present invention provides a compound traditional Chinese medicine preparation for improving the yield and quality of musk, comprising, by weight: 16 parts of Epimedium, 7 parts of Cuscuta, 11 parts of Morinda officinalis, 7 parts of Astragalus membranaceus, 14 parts of Rehmannia glutinosa, 9 parts of Angelica sinensis, 7 parts of Codonopsis pilosula, and 4 parts of Glycyrrhiza uralensis.

[0009] This invention provides a method for preparing a compound traditional Chinese medicine preparation for improving the yield and quality of musk, comprising the following steps: Weigh out the following ingredients by weight: Epimedium, Cuscuta, Morinda officinalis, Astragalus membranaceus, Rehmannia glutinosa, Angelica sinensis, Codonopsis pilosula, and Glycyrrhiza uralensis. Grind them into a fine powder and mix them evenly to obtain a compound traditional Chinese medicine preparation that improves the yield and quality of musk from forest musk deer.

[0010] Preferably, the particle size of the fine powder is less than 100 mesh.

[0011] The preparation method of the compound traditional Chinese medicine preparation of the present invention is simple, requiring only grinding and mixing.

[0012] The present invention also provides a method for applying a compound traditional Chinese medicine preparation for improving musk yield and quality, wherein the above-mentioned compound traditional Chinese medicine preparation for improving musk yield and quality is added as a feed additive to the daily diet of forest musk deer.

[0013] Preferably, the musk deer is a healthy adult male musk deer.

[0014] Preferably, the feeding begins 30 days before the musk deer's annual musk secretion period and continues for 30 days.

[0015] Preferably, the feeding dosage of the compound traditional Chinese medicine preparation is 2g or 4g per male musk deer per day.

[0016] This invention involves feeding the musk deer only trace amounts, without affecting their consumption of other foods. Since musk deer prefer bitter tastes and plants rich in secondary metabolites, and the eight traditional Chinese medicines used in this invention are rich in various plant secondary metabolites, they can promote food consumption. Furthermore, the compound traditional Chinese medicine preparation in this invention has low levels of crude protein and crude fat, and will not have a negative impact on the intestinal flora and physiological health of the musk deer.

[0017] Preferably, the addition method is to mix the compound traditional Chinese medicine preparation into the mixed feed of succulent feed and concentrated feed and stir evenly; the mass ratio of succulent feed to concentrated feed is 40:3.

[0018] Preferably, the succulent feed includes carrots, radishes, and pumpkins; the concentrated feed includes corn, wheat bran, and soybeans.

[0019] Preferably, the mass ratio of carrots, white radishes and pumpkins is 1:2:3; the mass ratio of corn, bran and soybeans is 6:3:1.

[0020] The application method of this invention only requires mixing the compound Chinese medicine preparation into the mixed feed of daily succulent feed and concentrated feed of the musk deer and stirring evenly. It does not require changing the existing feeding and management process, and is suitable for various captive musk deer farms, with good prospects for promotion.

[0021] In summary, the compound traditional Chinese medicine preparation for improving musk yield and quality, as well as its preparation and application method, provided by this invention have the following beneficial effects compared to traditional techniques: (1) The compound Chinese medicine preparation in this invention contains eight kinds of Chinese herbs, including Epimedium, Cuscuta, Morinda officinalis, Astragalus membranaceus, Rehmannia glutinosa, Angelica sinensis, Codonopsis pilosula and Glycyrrhiza uralensis. After grinding and compatibility, a small amount is added to the succulent feed of the musk deer. The musk deer can eat and live normally without affecting the captive musk deer. Moreover, the compound Chinese medicine used is mainly composed of herbs that invigorate yang and tonify the kidneys, supplemented by herbs that regulate qi and replenish blood. Glycyrrhiza uralensis is used to harmonize the herbs, which is intended to strengthen the body and nourish the source. The formula is comprehensive and inexpensive, and can regulate the physiological health level of the musk deer in multiple ways. At the same time, the added Chinese medicine additives are rich in a variety of nutrients and effective active ingredients, which can improve the production performance of animals, especially in promoting the reproductive function of animals. (2) The compound Chinese medicine preparation in this invention significantly improves both the yield and quality of musk. After being fed with the compound Chinese medicine preparation of this invention, the fresh weight of captive forest musk not only increases significantly, but also the content of muscone in musk increases significantly. (3) The compound Chinese medicine preparation in this invention regulates the activity of male musk gonads through multiple pathways of the hypothalamus-pituitary-gonadal axis, thereby increasing the musk secretion of forest musk deer and improving the immunity and stress level of forest musk deer, without having any adverse effects on forest musk deer.

[0022] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0023] Figure 1 A comparison chart of musk fresh weight yield of male musk deer in different groups (control group, 2g group and 4g group) of the compound traditional Chinese medicine preparation in feeding Example 1 of the present invention; Figure 2 This is a comparison chart of musk ketone content in male musk deer in different groups (control group, 2g group and 4g group) of the compound traditional Chinese medicine preparation in Example 1 of the present invention. Detailed Implementation

[0024] The technical method of the present invention will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specifically stated, the relative arrangement of steps, numerical expressions, and values ​​in these embodiments do not limit the scope of this application.

[0025] The following description of at least one exemplary embodiment is merely illustrative and is not intended to limit the scope of this application or its application or use.

[0026] In all the examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values.

[0027] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0028] The specific embodiments provided by this invention are as follows: Example 1 A method for preparing a compound traditional Chinese medicine preparation to improve the yield and quality of musk includes the following steps: Weigh out 16 parts of Epimedium, 7 parts of Cuscuta, 11 parts of Morinda officinalis, 7 parts of Astragalus membranaceus, 14 parts of Rehmannia glutinosa, 9 parts of Angelica sinensis, 7 parts of Codonopsis pilosula, and 4 parts of Glycyrrhiza uralensis according to the dry weight ratio. Grind them into 100-mesh fine powder using a high-speed grinder. Place them in a mixer and stir until evenly mixed to obtain a compound Chinese medicine preparation. Seal and store for later use.

[0029] Animal feeding experiments Experimental animals: Forty healthy adult male musk deer were selected as experimental subjects and randomly divided into four groups of 10 each. There were no significant differences in baseline conditions (age, weight, and previous musk production) among the groups.

[0030] Experimental grouping and treatment: The experiment was divided into a control group (0g group), a 2g group, a 4g group and a 6g group.

[0031] In addition to feeding basic forage, the control group (0g group) was fed only mixed feed daily. The 2g group received 2g / animal of the compound traditional Chinese medicine preparation from Example 1 of this invention added to their mixed feed daily. The 4g group received 4g / animal of the compound traditional Chinese medicine preparation from Example 1 of this invention added to their mixed feed daily. The 6g group received 6g / animal of the compound traditional Chinese medicine preparation from Example 1 of this invention added to their mixed feed daily.

[0032] The mixed feed consisted of a mixture of succulent feed and concentrated feed for the musk deer (the mass ratio of succulent feed to concentrated feed was 40:3). The succulent feed included carrots, white radishes, and pumpkins in a mass ratio of 1:2:3, while the concentrated feed included corn, wheat bran, and soybeans in a mass ratio of 6:3:1. The weighed compound traditional Chinese medicine preparations (groups 2g, 4g, and 6g) were added to the mixed feed, stirred thoroughly, and fed to the musk deer.

[0033] Feeding method: Begin feeding 30 days before the musk deer's musk secretion period (May-July) and continue feeding for 30 days, feeding once every morning. During the feeding period, feed the musk deer a mixture of dried or fresh leaves and warm water as usual.

[0034] Observation records: During the feeding period, the feeding behavior, mental state, and fecal characteristics of each group of musk deer were observed daily. The 6g group began to refuse food or reduce food intake from days 5 to 7, possibly due to the odor of the Chinese herbal preparation affecting normal feeding. This group was not included in the subsequent statistical analysis of musk production and muscone content. The other groups had normal feeding and no diseases or abnormal behaviors were observed.

[0035] Musk Sample Collection: Musk is a secretion from the musk glands within the musk sac of musk deer. Musk secretion in forest musk deer begins and ends between May and July. Initially, the secreted musk is a pale yellow, oily liquid. This initial musk gradually matures within the musk sac through microbial action, turning into a reddish-brown oily liquid or semi-solid by August. By October, it becomes mature musk, appearing as a solid brown or dark brown musk containing a small amount of granular material, and remains in this state until the following year's re-secretion. Therefore, in February of the following year, musk was collected from male musk deer in both the control and experimental groups (2g and 4g groups) using standard artificial live musk extraction methods. The fresh weight was recorded immediately after collection. Simultaneously, a suitable amount of musk sample was taken from each deer, placed in a cryovial, and quickly stored under dry ice for subsequent muscone content analysis.

[0036] Muskone content detection Musk sample pretreatment: To prevent high temperatures from affecting the volatile components in musk, lyophilization was used to dry the musk. The musk sample was pre-frozen overnight at -80℃, then the sample tray was placed in a pre-freezing rack and freeze-dried for 48 hours. After removal, it was placed in a desiccator and weighed again. This process was repeated until the difference between two weighings did not exceed 2 mg, indicating complete drying.

[0037] Weigh an appropriate amount of freeze-dried and completely dehydrated musk sample and add it to a sterile centrifuge tube. Add dichloromethane. Vortex thoroughly to dissolve the musk. Filter the dichloromethane extract through a 0.22 μm filter into a dedicated sample vial, ready for instrumental analysis.

[0038] The conditions for the instrumental analysis were as follows: column: HP-5 (30m×0.32mm×0.25μm); temperature program: initial temperature 70℃, hold for 1 min, increase to 280℃ at 40℃ / min, hold for 3 min; injection port temperature: 250℃; carrier gas: nitrogen, flow rate 1.0mL / min; split injection: split ratio 30:1; detector: FID, detector temperature: 250℃.

[0039] The formula for calculating muscone content is as follows: ; Where W is the content of muscone in the musk sample, in mg / kg; C is the concentration of muscone in the musk sample test solution, in mg / L; C0 is the concentration of muscone in the control group, in mg / L; V is the final volume, in mL; and N is the dilution factor. m The mass of the musk sample is expressed in grams.

[0040] Statistical analysis of test results Musk yield results: Adjusted for fresh weight based on a 35% moisture content, the average musk yield was 13.06 ± 1.59 g in the control group (0 g group), 14.52 ± 0.55 g in the 2 g group (an increase of 11.20% compared to the control group), and 16.17 ± 0.68 g in the 4 g group (an increase of 23.85% compared to the control group). Details are as follows... Figure 1 As shown, Figure 1 middle This indicates a significant difference. The difference is considered highly significant. Statistical analysis shows that the 2g group had a significant difference from the control group, and the 4g group had a highly significant difference from the control group. That is, the 2g group significantly increased the musk production of male musk deer, and the 4g group significantly increased the musk production of male musk deer.

[0041] Results of muscone content: The average muscone content in the control group (0g group) was 2.15±0.74%, the average muscone content in the 2g group was 3.19±0.55% (48.78% higher than the control group), and the average muscone content in the 4g group was 2.12±0.71% (no significant difference from the control group). (Details are as follows...) Figure 2 As shown, Figure 2 In ns, the difference is not significant. The difference is significant. The results of muscone content analysis indicate that the 2g group significantly improved the musk quality of male musk deer.

[0042] Experimental Conclusion: The compound traditional Chinese medicine preparation in Example 1, at a feeding dose of 2g per male musk deer per day, can significantly improve both musk yield and quality in captive forest musk deer; at a feeding dose of 4g per male musk deer per day, it can significantly improve musk yield, but has no significant effect on muscone content. Considering the dual improvement effect on yield and quality, 2g per male musk deer per day is the optimal feeding dose.

[0043] Economic benefits Based on a daily feeding dosage of 2g per male musk deer and 30 days of feeding before the musk secretion period each year, the cost of the compound traditional Chinese medicine preparation per deer per year is only 1.94 yuan. With a fresh musk purchase price of 500 yuan / g, the profit per male musk deer increases by approximately 1500 yuan. For a medium-sized musk deer farm with 100 male musk deer, this could increase annual income by 150,000 yuan, a substantial increase in revenue. Furthermore, the National Pharmacopoeia stipulates that the muscone content in natural musk cannot be less than 2%. This invention can significantly improve the quality of musk, ensuring it meets the standards stipulated in the National Pharmacopoeia.

[0044] This invention provides a compound traditional Chinese medicine preparation for improving musk yield and quality, along with its preparation and application methods. Through the rational combination of eight traditional Chinese medicines—Epimedium, Cuscuta, Morinda officinalis, Astragalus membranaceus, Rehmannia glutinosa, Angelica sinensis, Codonopsis pilosula, and Glycyrrhiza uralensis—based on the principles of strengthening the body's foundation, tonifying the kidneys and boosting yang, and regulating qi and blood, this formula regulates the activity of the male musk gonads through multiple pathways of the hypothalamus-pituitary-gonadal axis. This comprehensively improves the musk yield and quality of forest musk deer, effectively overcoming many shortcomings of current technologies, such as stress responses caused by injecting exogenous hormones, limited effects of existing traditional Chinese medicine formulas, incomplete effects of single flavonoid compounds, and digestive diseases caused by high-protein feed. This provides a safe, efficient, and economical solution for improving musk performance in captive forest musk deer farming, possessing significant industrial practical value and broad application prospects.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A compound traditional Chinese medicine preparation for improving the yield and quality of musk, characterized in that, By weight, it includes: 16 parts of Epimedium, 7 parts of Cuscuta, 11 parts of Morinda officinalis, 7 parts of Astragalus membranaceus, 14 parts of Rehmannia glutinosa, 9 parts of Angelica sinensis, 7 parts of Codonopsis pilosula, and 4 parts of Glycyrrhiza uralensis.

2. A method for preparing a compound traditional Chinese medicine preparation to improve the yield and quality of musk, characterized in that, The preparation of the compound traditional Chinese medicine formulation for improving musk yield and quality according to claim 1 includes the following steps: Weigh out the following ingredients by weight: Epimedium, Cuscuta, Morinda officinalis, Astragalus membranaceus, Rehmannia glutinosa, Angelica sinensis, Codonopsis pilosula, and Glycyrrhiza uralensis. Grind them into a fine powder and mix them evenly to obtain a compound traditional Chinese medicine preparation that improves the yield and quality of musk from forest musk deer.

3. The method for preparing the compound traditional Chinese medicine preparation for improving musk yield and quality according to claim 2, characterized in that, The particle size of the fine powder is below 100 mesh.

4. A method for applying a compound traditional Chinese medicine preparation to improve the yield and quality of musk, characterized in that, The compound traditional Chinese medicine preparation for improving musk yield and quality as described in claim 1 is added as a feed additive to the daily diet of forest musk deer.

5. The application method of the compound traditional Chinese medicine preparation for improving musk yield and quality according to claim 4, characterized in that, The musk deer mentioned are healthy adult males.

6. The application method of the compound traditional Chinese medicine preparation for improving musk yield and quality according to claim 5, characterized in that, The feeding begins 30 days before the musk deer's annual musk secretion period and continues for 30 days.

7. The application method of the compound traditional Chinese medicine preparation for improving musk yield and quality according to claim 5, characterized in that, The feeding dosage of the compound traditional Chinese medicine preparation is 2g or 4g per male musk deer per day.

8. The application method of the compound traditional Chinese medicine preparation for improving musk yield and quality according to claim 4, characterized in that, The addition method involves mixing the compound traditional Chinese medicine preparation into the mixed feed of succulent feed and concentrated feed and stirring evenly; the mass ratio of succulent feed to concentrated feed is 40:

3.

9. The application method of the compound traditional Chinese medicine preparation for improving musk yield and quality according to claim 8, characterized in that, The succulent feed includes carrots, radishes, and pumpkins; the concentrated feed includes corn, wheat bran, and soybeans.

10. The application method of the compound traditional Chinese medicine preparation for improving musk yield and quality according to claim 8, characterized in that, The mass ratio of carrots, white radishes and pumpkins is 1:2:3; the mass ratio of corn, bran and soybeans is 6:3:1.

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