A composition for tonifying qi and blood and tonifying kidney and a preparation method thereof

Abstract

This invention belongs to the field of pharmaceutical technology, specifically relating to a composition for tonifying qi and blood and tonifying the kidneys, and its preparation method. The composition, by weight, comprises: 5-25 parts of Panax notoginseng, 3-18 parts of Astragalus membranaceus, 10-20 parts of Lycium barbarum, 8-40 parts of Bitter Thorn Flower, 2-15 parts of Litsea cubeba, 5-18 parts of Gastrodia elata, 2-9 parts of Ginkgo biloba, and 3-12 parts of Dendrobium officinale. The preparation method is as follows: S1, weigh and pulverize the raw materials; S2, extract Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower, and Dendrobium officinale with water and then with alcohol, concentrating and drying the alcohol extract to obtain the first extract; S3, extract Litsea cubeba, Gastrodia elata, and Ginkgo biloba with water and then with alcohol, concentrating and drying the alcohol extract to obtain the second extract; S4, concentrate and dry the water extracts from S2 and S3 to obtain the third extract; S5, combine Panax notoginseng, the first extract, the second extract, and the third extract to obtain the final product. The composition obtained by this invention has the effects of enhancing immunity, relieving physical fatigue, replenishing qi and blood, and strengthening qi and consolidating essence.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical technology, specifically relating to a composition for replenishing qi and blood and tonifying the kidneys, and its preparation method. Background Technology

[0002] With the fast pace of modern life, many people suffer from qi and blood deficiency and kidney essence depletion, manifesting as fatigue, shortness of breath, spontaneous sweating, decreased immunity, dizziness, palpitations, insomnia, lower back and knee weakness, aversion to cold or hot flashes, tinnitus, and decreased sexual function. Long-term qi and blood deficiency and kidney deficiency are mutually reinforcing, severely impacting quality of life.

[0003] Although there are some traditional Chinese medicine products on the market that replenish qi and blood or tonify the kidneys, most of them have only one function, or they tend to focus on replenishing qi and blood while neglecting kidney tonification, or they tend to focus on warming the kidneys and strengthening yang while neglecting qi and blood regulation, making it difficult to achieve the synergistic effect of replenishing both qi and blood and tonifying the kidneys. Summary of the Invention

[0004] One of the technical problems to be solved by the present invention is to provide a composition for replenishing qi and blood and tonifying the kidneys, which, by weight, consists of the following raw materials: 5-25 parts of Panax notoginseng, 3-18 parts of Astragalus membranaceus, 10-20 parts of Lycium barbarum, 8-40 parts of Bitter Thorn Flower, 2-15 parts of Litsea cubeba, 5-18 parts of Gastrodia elata, 2-9 parts of Ginkgo biloba, and 3-12 parts of Dendrobium officinale.

[0005] In the composition for replenishing qi and blood and tonifying the kidneys provided by the present invention, preferably, it is composed of the following raw materials in parts by weight: 5-16 parts of Panax notoginseng, 6-10 parts of Astragalus membranaceus, 12-15 parts of Lycium barbarum, 10-30 parts of Bitter Thorn Flower, 5-8 parts of Litsea cubeba, 8-15 parts of Gastrodia elata, 3-5 parts of Ginkgo biloba and 5-10 parts of Dendrobium officinale.

[0006] In the qi-tonifying and kidney-tonifying composition provided by the present invention, more preferably, it is composed of the following raw materials in parts by weight: 12 parts of Panax notoginseng, 7 parts of Astragalus membranaceus, 13 parts of Lycium barbarum, 25 parts of Bitter Thorn Flower, 7 parts of Litsea cubeba, 11 parts of Gastrodia elata, 3 parts of Ginkgo biloba and 7 parts of Dendrobium officinale.

[0007] More preferably, the composition for replenishing qi and blood and tonifying the kidneys provided by the present invention is composed of the following raw materials in parts by weight: 12 parts of Panax notoginseng, 7 parts of Astragalus membranaceus, 13 parts of Lycium barbarum, 25 parts of Bitter Thorn Flower, 7 parts of Litsea cubeba, 11 parts of Gastrodia elata, 3 parts of Ginkgo biloba, 7 parts of Dendrobium officinale and 5 parts of Chaenomeles speciosa.

[0008] Another technical problem to be solved by the present invention is to provide a method for preparing a composition for replenishing qi and blood and tonifying the kidneys, comprising the following steps:

[0009] S1. Weigh the following raw materials according to the following weight proportions: 5-25 parts of Panax notoginseng, 3-18 parts of Astragalus membranaceus, 10-20 parts of Lycium barbarum, 8-40 parts of Bitter Thorn Flower, 2-15 parts of Litsea cubeba, 5-18 parts of Gastrodia elata, 2-9 parts of Ginkgo biloba, and 3-12 parts of Dendrobium officinale; and pulverize each raw material separately.

[0010] S2. Place the crushed Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower and Dendrobium officinale into a container, add water, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 80-95% vol ethanol, heat and reflux for 2-4 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, crush, and obtain the first extract;

[0011] S3. Put the crushed Litsea cubeba, Gastrodia elata and Ginkgo biloba into a container, add water, decoct, filter to obtain the third decoction and the third residue, put the third residue into a reflux extraction device, add 50-75% vol ethanol, heat and reflux for 2-5 hours, filter to obtain the fourth decoction and the fourth residue; distill the fourth decoction under reduced pressure, concentrate and dry, crush to obtain the second extract.

[0012] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0013] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0014] In the preparation method of the composition for replenishing qi and blood and tonifying the kidneys provided by the present invention, preferably, in step S1, 2 to 5 parts of papaya are weighed and crushed.

[0015] In the preparation method of the composition for replenishing qi and blood and tonifying the kidneys provided by the present invention, it is even more preferred that the papaya is in the form of 5 parts by weight.

[0016] In the preparation method of the composition for replenishing qi and blood and tonifying the kidneys provided by the present invention, more preferably, the pulverized papaya is treated in the same way as the raw materials in step S3.

[0017] In the preparation method of the composition for replenishing qi and blood and tonifying the kidney provided by the present invention, it is further preferred that the ethanol concentration in step S2 is 90% vol.

[0018] In the preparation method of the composition for replenishing qi and blood and tonifying the kidney provided by the present invention, more preferably, the ethanol concentration in step S3 is 60% vol.

[0019] Panax notoginseng (Burk.) FHChen, a plant belonging to the Araliaceae family, is a dried root and rhizome. It is harvested in autumn before flowering, washed, and the main root, lateral roots, and rhizome are separated and dried. It is sweet and slightly bitter, and warm in nature. It enters the liver and stomach meridians. It disperses blood stasis, stops bleeding, reduces swelling, and relieves pain. It is used for hemoptysis, hematemesis, epistaxis, hematochezia, metrorrhagia, traumatic bleeding, chest and abdominal pain, and swelling and pain from falls.

[0020] Astragalus is the dried root of *Astragalus membranaceus* (Fisch.) Bge. var. *mongholicus* (Bge.) Hsiao or *Astragalus membranaceus* (Fisch.) Bge., belonging to the Fabaceae family. It is harvested in spring and autumn, with the fibrous roots and root head removed, and then sun-dried. It is sweet and slightly warm in nature. It enters the Lung and Spleen meridians. Its functions include tonifying Qi and raising Yang, consolidating the exterior and stopping sweating, promoting diuresis and reducing swelling, generating fluids and nourishing blood, promoting circulation and relieving pain, promoting pus drainage, and astringing sores and promoting tissue regeneration. It is used for Qi deficiency and fatigue, poor appetite and loose stools, Qi sinking, chronic diarrhea and rectal prolapse, hematochezia and metrorrhagia, spontaneous sweating due to exterior deficiency, edema due to Qi deficiency, internal heat and thirst, blood deficiency and chlorosis, hemiplegia, numbness and pain, carbuncles that are difficult to heal, and chronic ulcers that do not heal.

[0021] Goji berries are the dried, ripe fruits of *Lycium barbarum* L., a plant in the Solanaceae family. They are harvested in summer and autumn when the fruits turn red, dried with hot air, and the fruit stalks are removed. Alternatively, they can be air-dried until the skin wrinkles, then sun-dried and the fruit stalks are removed. They are sweet and neutral in nature. They enter the liver and kidney meridians. They nourish the liver and kidneys, benefit essence and improve eyesight. They are used for general weakness and deficiency of essence, soreness and pain in the lower back and knees, dizziness and tinnitus, impotence and seminal emission, internal heat and thirst, anemia and chlorosis, and blurred vision.

[0022] Bitter thorn flower is the flower of Sophora davidii (Franch.) Skeels, a plant in the legume family. It is harvested in spring and dried. It tastes bitter and is cold in nature. It enters the liver and bladder meridians and has the functions of clearing heat and detoxifying, cooling blood and reducing swelling.

[0023] Litsea pungens Hemsl., a plant belonging to the genus Litsea in the family Lauraceae, is the dried root of the plant, harvested in autumn. It has a pungent and warm nature, and enters the spleen, stomach, and lung meridians. It warms the middle jiao (spleen and stomach), regulates qi, dispels cold, and relieves pain; it is mainly used to treat rheumatic joint pain, malaria, and dysmenorrhea.

[0024] Gastrodia elata is the dried tuber of the orchid Gastrodia elata Bl. It is harvested from after the beginning of winter to before the Qingming Festival of the following year, immediately washed, steamed thoroughly, and dried in an open, low-temperature environment. It is sweet and neutral in nature. It enters the liver meridian. It calms wind and stops spasms, suppresses liver yang, and dispels wind and unblocks the meridians. It is used for infantile convulsions, epileptic seizures, tetanus, headaches and dizziness, hemiplegia, numbness of limbs, and rheumatic pain.

[0025] Ginkgo seeds are the dried, mature seeds of Ginkgo biloba L., a plant in the Ginkgoaceae family. They are harvested in autumn when mature, the fleshy outer seed coat is removed, the seeds are washed, briefly steamed or boiled, and then dried. They have the effects of astringing the lungs and relieving asthma, stopping leukorrhea and reducing urination. They are mainly used to treat cough with excessive phlegm, leukorrhea, enuresis, and frequent urination.

[0026] Dendrobium officinale Kimura & Mig, a plant belonging to the genus Dendrobium in the family Orchidaceae, is known as iron-skin dendrobium. It is believed to have the effects of tonifying the five internal organs, strengthening yin and replenishing essence, nourishing blood and qi, and relieving numbness.

[0027] Papaya is the dried, nearly mature fruit of *Chaenomeles speciosa* (Sweet) Nakai, a plant in the Rosaceae family. It is harvested in summer and autumn when the fruit is greenish-yellow, scalded in boiling water until the outer skin turns grayish-white, then longitudinally split in half and sun-dried. It is sour and warm in nature. It enters the liver and spleen meridians. It relaxes muscles and tendons, harmonizes the stomach, and resolves dampness. It is used for damp-bi syndrome with contractures, lower back and knee pain, summer-heat vomiting and diarrhea, muscle spasms and pain, and beriberi with edema.

[0028] Using the above technical solution and following the principles of principal, assistant, and adjuvant herbs, Astragalus membranaceus and Lycium barbarum are the principal herbs, providing the material basis for replenishing qi and blood and kidney essence; Panax notoginseng and Dendrobium officinale are the assistant herbs, promoting blood circulation, nourishing yin, and preventing dryness, thus enhancing the efficacy of the principal herbs; Bitter thorn flower, Litsea cubeba, Gastrodia elata, Ginkgo biloba, and Chaenomeles speciosa are the adjuvant herbs. Bitter thorn flower enhances immunity, Litsea cubeba enhances kidney tonification, and Bitter thorn flower and Litsea cubeba work synergistically to replenish blood and counteract each other's cold and heat properties; Gastrodia elata, Ginkgo biloba, and Chaenomeles speciosa calm the liver, strengthen the kidneys, relax muscles, and treat concurrent symptoms. The composition provided by this invention has the effects of enhancing immunity, relieving physical fatigue, replenishing qi and blood, and strengthening qi and essence. In the preparation method of this invention, the raw materials other than Panax notoginseng are pulverized, extracted with water and alcohol, and then mixed with the pulverized Panax notoginseng to maximize the efficacy of the active ingredients in the raw materials. Detailed Implementation

[0029] The specific embodiments of the present invention will be further described below. It should be noted that these descriptions are for the purpose of aiding understanding the present invention, but do not constitute a limitation thereof. Furthermore, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0030] Example 1

[0031] This embodiment provides a composition for replenishing qi and blood and tonifying the kidneys, which consists of the following raw materials by weight: 25 parts of Panax notoginseng, 3 parts of Astragalus membranaceus, 20 parts of Lycium barbarum, 8 parts of Bitter Thorn Flower, 15 parts of Litsea cubeba, 5 parts of Gastrodia elata, 2 parts of Ginkgo biloba and 12 parts of Dendrobium officinale.

[0032] Its preparation method includes the following steps:

[0033] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0034] S2. The pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower and Dendrobium officinale were put into a container, and water was added at a material-to-liquid ratio of 1:5. The mixture was decocted, filtered, and the first decoction and the first residue were obtained. The first residue was put into a reflux extraction device, and 80% vol ethanol was added at a material-to-liquid ratio of 1:3. The mixture was heated to reflux at 65°C for 4 hours and filtered to obtain the second decoction and the second residue. The second decoction was distilled under reduced pressure, concentrated and dried, and pulverized to obtain the first extract.

[0035] S3. Place the crushed Litsea cubeba, Gastrodia elata, and Ginkgo biloba into a container, add water, decoct, and filter to obtain the third medicinal liquid and the third medicinal residue. Put the third medicinal residue into a reflux extraction device, add 75% vol ethanol at a material-to-liquid ratio of 1:5, heat at 75°C and reflux for 2 hours, and filter to obtain the fourth medicinal liquid and the fourth medicinal residue. Distill the fourth medicinal liquid under reduced pressure, concentrate and dry, crush, and obtain the second extract.

[0036] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0037] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0038] Example 2

[0039] This embodiment provides a composition for replenishing qi and blood and tonifying the kidneys, which consists of the following raw materials by weight: 5 parts of Panax notoginseng, 18 parts of Astragalus membranaceus, 10 parts of Lycium barbarum, 40 parts of Bitter Thorn Flower, 2 parts of Litsea cubeba, 18 parts of Gastrodia elata, 9 parts of Ginkgo biloba and 3 parts of Dendrobium officinale.

[0040] Its preparation method includes the following steps:

[0041] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0042] S2. Place the pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower, and Dendrobium officinale into a container, add water at a material-to-liquid ratio of 1:4, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 95% vol ethanol at a material-to-liquid ratio of 1:3, heat at 75℃ and reflux for 2 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, pulverize, and obtain the first extract;

[0043] S3. Place the crushed Litsea cubeba, Gastrodia elata, and Ginkgo biloba into a container, add water, decoct, and filter to obtain the third medicinal liquid and the third medicinal residue. Put the third medicinal residue into a reflux extraction device, add 50% vol ethanol at a material-to-liquid ratio of 1:6, heat at 70℃ and reflux for 5 hours, and filter to obtain the fourth medicinal liquid and the fourth medicinal residue. Distill the fourth medicinal liquid under reduced pressure, concentrate and dry, crush, and obtain the second extract.

[0044] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0045] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0046] Example 3

[0047] This embodiment provides a composition for replenishing qi and blood and tonifying the kidneys, which consists of the following raw materials in parts by weight: 16 parts of Panax notoginseng, 10 parts of Astragalus membranaceus, 12 parts of Lycium barbarum, 30 parts of Bitter Thorn Flower, 5 parts of Litsea cubeba, 15 parts of Gastrodia elata, 5 parts of Ginkgo biloba and 5 parts of Dendrobium officinale.

[0048] Its preparation method includes the following steps:

[0049] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0050] S2. Place the pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower, and Dendrobium officinale into a container, add water at a material-to-liquid ratio of 1:4, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 90% vol ethanol at a material-to-liquid ratio of 1:3, heat at 68℃ and reflux for 3 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, pulverize, and obtain the first extract;

[0051] S3. Place the crushed Litsea cubeba, Gastrodia elata, and Ginkgo biloba into a container, add water, decoct, and filter to obtain the third medicinal liquid and the third medicinal residue. Put the third medicinal residue into a reflux extraction device, add 60% vol ethanol at a material-to-liquid ratio of 1:5, heat at 72℃ and reflux for 4 hours, and filter to obtain the fourth medicinal liquid and the fourth medicinal residue. Distill the fourth medicinal liquid under reduced pressure, concentrate and dry, crush, and obtain the second extract.

[0052] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0053] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0054] Example 4

[0055] This embodiment provides a composition for replenishing qi and blood and tonifying the kidneys, which consists of the following raw materials in parts by weight: 16 parts of Panax notoginseng, 6 parts of Astragalus membranaceus, 15 parts of Lycium barbarum, 10 parts of Bitter Thorn Flower, 8 parts of Litsea cubeba, 8 parts of Gastrodia elata, 3 parts of Ginkgo biloba and 10 parts of Dendrobium officinale.

[0056] Its preparation method includes the following steps:

[0057] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0058] S2. The pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower and Dendrobium officinale were put into a container, and water was added at a material-to-liquid ratio of 1:6. The mixture was decocted, filtered, and the first decoction and the first residue were obtained. The first residue was put into a reflux extraction device, and 85% vol ethanol was added at a material-to-liquid ratio of 1:5. The mixture was heated to reflux at 72°C for 3 hours and filtered to obtain the second decoction and the second residue. The second decoction was distilled under reduced pressure, concentrated and dried, and pulverized to obtain the first extract.

[0059] S3. Place the crushed Litsea cubeba, Gastrodia elata, and Ginkgo biloba into a container, add water, decoct, and filter to obtain the third medicinal liquid and the third medicinal residue. Put the third medicinal residue into a reflux extraction device, add 65% vol ethanol at a material-to-liquid ratio of 1:6, heat at 74℃ and reflux for 3 hours, and filter to obtain the fourth medicinal liquid and the fourth medicinal residue. Distill the fourth medicinal liquid under reduced pressure, concentrate and dry, crush, and obtain the second extract.

[0060] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0061] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0062] Example 5

[0063] This embodiment provides a composition for replenishing qi and blood and tonifying the kidneys, which consists of the following raw materials in parts by weight: 12 parts of Panax notoginseng, 7 parts of Astragalus membranaceus, 13 parts of Lycium barbarum, 25 parts of Bitter Thorn Flower, 7 parts of Litsea cubeba, 11 parts of Gastrodia elata, 3 parts of Ginkgo biloba and 7 parts of Dendrobium officinale.

[0064] Its preparation method includes the following steps:

[0065] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0066] S2. Place the pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower, and Dendrobium officinale into a container, add water at a material-to-liquid ratio of 1:4, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 90% vol ethanol at a material-to-liquid ratio of 1:5, heat at 73℃ and reflux for 3 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, pulverize, and obtain the first extract;

[0067] S3. Place the crushed Litsea cubeba, Gastrodia elata, and Ginkgo biloba into a container, add water, decoct, and filter to obtain the third medicinal liquid and the third medicinal residue. Put the third medicinal residue into a reflux extraction device, add 68% vol ethanol at a material-to-liquid ratio of 1:6, heat at 71℃ and reflux for 4 hours, and filter to obtain the fourth medicinal liquid and the fourth medicinal residue. Distill the fourth medicinal liquid under reduced pressure, concentrate and dry, crush, and obtain the second extract.

[0068] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0069] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0070] Example 6

[0071] This embodiment provides a composition for replenishing qi and blood and tonifying the kidneys, which consists of the following raw materials in parts by weight: 12 parts of Panax notoginseng, 7 parts of Astragalus membranaceus, 13 parts of Lycium barbarum, 25 parts of Bitter Thorn Flower, 7 parts of Litsea cubeba, 11 parts of Gastrodia elata, 3 parts of Ginkgo biloba, 7 parts of Dendrobium officinale, and 2 parts of Chaenomeles speciosa.

[0072] Its preparation method includes the following steps:

[0073] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0074] S2. Place the pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower, and Dendrobium officinale into a container, add water at a material-to-liquid ratio of 1:4, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 90% vol ethanol at a material-to-liquid ratio of 1:5, heat at 73℃ and reflux for 3 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, pulverize, and obtain the first extract;

[0075] S3. Place the crushed Litsea cubeba, Gastrodia elata, Ginkgo biloba and Chaenomeles speciosa into a container, add water, decoct, filter to obtain the third decoction and the third residue. Put the third residue into a reflux extraction device, add 68% vol ethanol at a material-to-liquid ratio of 1:6, heat at 71℃ and reflux for 4 hours, filter to obtain the fourth decoction and the fourth residue. Distill the fourth decoction under reduced pressure, concentrate and dry, crush to obtain the second extract.

[0076] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0077] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0078] Example 7

[0079] This embodiment provides a composition for replenishing qi and blood and tonifying the kidneys, which consists of the following raw materials in parts by weight: 12 parts of Panax notoginseng, 7 parts of Astragalus membranaceus, 13 parts of Lycium barbarum, 25 parts of Bitter Thorn Flower, 7 parts of Litsea cubeba, 11 parts of Gastrodia elata, 3 parts of Ginkgo biloba, 7 parts of Dendrobium officinale, and 5 parts of Chaenomeles speciosa.

[0080] Its preparation method includes the following steps:

[0081] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0082] S2. Place the pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower, and Dendrobium officinale into a container, add water at a material-to-liquid ratio of 1:4, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 90% vol ethanol at a material-to-liquid ratio of 1:5, heat at 73℃ and reflux for 3 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, pulverize, and obtain the first extract;

[0083] S3. Place the crushed Litsea cubeba, Gastrodia elata, Ginkgo biloba and Chaenomeles speciosa into a container, add water, decoct, filter to obtain the third decoction and the third residue. Put the third residue into a reflux extraction device, add 68% vol ethanol at a material-to-liquid ratio of 1:6, heat at 71℃ and reflux for 4 hours, filter to obtain the fourth decoction and the fourth residue. Distill the fourth decoction under reduced pressure, concentrate and dry, crush to obtain the second extract.

[0084] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0085] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

[0086] Comparative Example 1

[0087] This comparative example provides a composition, by weight, consisting of the following raw materials: 12 parts Panax notoginseng, 7 parts Astragalus membranaceus, 13 parts Lycium barbarum, 7 parts Litsea cubeba, 11 parts Gastrodia elata, 3 parts Ginkgo biloba, and 7 parts Dendrobium officinale.

[0088] Its preparation method includes the following steps:

[0089] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0090] S2. Place the pulverized Astragalus membranaceus, Lycium barbarum, and Dendrobium officinale into a container, add water at a material-to-liquid ratio of 1:4, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 90% vol ethanol at a material-to-liquid ratio of 1:5, heat at 73℃ and reflux for 3 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, pulverize, and obtain the first extract;

[0091] S3. Place the crushed Litsea cubeba, Gastrodia elata, and Ginkgo biloba into a container, add water, decoct, and filter to obtain the third medicinal liquid and the third medicinal residue. Put the third medicinal residue into a reflux extraction device, add 68% vol ethanol at a material-to-liquid ratio of 1:6, heat at 71℃ and reflux for 4 hours, and filter to obtain the fourth medicinal liquid and the fourth medicinal residue. Distill the fourth medicinal liquid under reduced pressure, concentrate and dry, crush, and obtain the second extract.

[0092] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0093] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain the composition.

[0094] Comparative Example 2

[0095] This comparative example provides a composition, by weight, consisting of the following raw materials: 12 parts Panax notoginseng, 7 parts Astragalus membranaceus, 13 parts Lycium barbarum, 25 parts Bitter Thorn Flower, 11 parts Gastrodia elata, 3 parts Ginkgo biloba, and 7 parts Dendrobium officinale.

[0096] Its preparation method includes the following steps:

[0097] S1. Weigh the above raw materials according to the weight proportions, and crush each raw material separately;

[0098] S2. Place the pulverized Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower, and Dendrobium officinale into a container, add water at a material-to-liquid ratio of 1:4, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 90% vol ethanol at a material-to-liquid ratio of 1:5, heat at 73℃ and reflux for 3 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, pulverize, and obtain the first extract;

[0099] S3. Put the crushed Gastrodia elata and Ginkgo biloba into a container, add water, decoct, filter to obtain the third medicinal liquid and the third medicinal residue. Put the third medicinal residue into a reflux extraction device, add 68% vol ethanol at a material-to-liquid ratio of 1:6, heat at 71℃ and reflux for 4 hours, filter to obtain the fourth medicinal liquid and the fourth medicinal residue. Distill the fourth medicinal liquid under reduced pressure, concentrate and dry, crush to obtain the second extract.

[0100] S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract.

[0101] S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain the composition.

[0102] Experimental Example 1

[0103] One hundred SPF-grade adult Balb / c mice (weighing 18–22 g) were randomly divided into 10 groups of 10 mice each: a blank control group, groups 1 to 7, comparative example 1, and comparative example 2. Each mouse in groups 1 to 7, comparative example 1, and comparative example 2 was administered 0.1 g / mouse via gavage once daily (0.1 g of each composition was dissolved in 0.8 mL of sterile water before gavage). This treatment continued for 30 days.

[0104] Mice should be kept in the following conditions: temperature 20–26℃, relative humidity 40%–70%, and alternating light and dark cycles for 12 hours. Free access to water should be provided, and the mice should be fed a standard maintenance diet.

[0105] Thirty minutes after the last gavage, each mouse was loaded with lead at the base of its tail with 5% of its body weight. The mice were then placed in a 60×60×40cm swimming tank with a water depth of 35cm and a water temperature of 25±1℃. The time from the start of swimming to death was recorded, i.e., the weighted swimming time of the mice. The results are shown in Table 1.

[0106] Table 1. Swimming time under load for mice in each group ( ±s,n=10)

[0107]

[0108] As can be seen from Table 1, the weight-bearing swimming time of mice in Examples 1 to 7, and Comparative Examples 1 and 2 was significantly longer than that of the blank control group, indicating that the compositions obtained in Examples 1 to 7 can effectively alleviate physical fatigue in mice.

[0109] Experimental Example 2

[0110] One hundred SPF-grade adult Balb / c mice (weighing 18–22 g) were randomly divided into 10 groups of 10 mice each: a blank control group, groups 1 to 7, comparative example 1, and comparative example 2. Each mouse in groups 1 to 7, comparative example 1, and comparative example 2 was administered 0.1 g / mouse via gavage once daily (0.1 g of each composition was dissolved in 0.8 mL of sterile water before gavage). This treatment continued for 30 days.

[0111] Mice should be kept in the following conditions: temperature 20–26℃, relative humidity 40%–70%, and alternating light and dark cycles for 12 hours. Free access to water should be provided, and the mice should be fed a standard maintenance diet.

[0112] Thirty days after gavage, the spleens of mice in each group were aseptically harvested and placed in a petri dish containing an appropriate amount of sterile Hank's solution. The spleens were gently ground with forceps to prepare a single-cell suspension. The suspension was filtered through a 200-mesh sieve, washed twice with Hank's solution, and centrifuged for 10 min each time (1000 r / min). The cells were then resuspended in 1 mL of complete culture medium, and the viable cell count was determined by tyrosine blue staining (the cell count should be above 95%). The cell concentration was adjusted to 3 × 10⁶ cells / mL. 6 per mL.

[0113] Each spleen cell suspension was divided into two wells and added to a 24-well plate, 1 mL per well. One well contained 75 μL of 100 μg / mL ConA solution (equivalent to 7.5 μg / mL), and the other well served as a control. The plates were incubated in a 5% CO2, 37℃ CO2 incubator for 72 h. Four hours before the end of the incubation period, 0.7 mL of supernatant was gently aspirated from each well, and 0.7 mL of RPMI 1640 medium without fetal bovine serum was added. Simultaneously, 50 μL of MTT (5 mg / mL) per well was added, and the plates were incubated for another 4 h. After the incubation period, 1 mL of acidic isopropanol was added to each well, and the mixture was pipetted to dissolve the purple crystals completely. The samples were then aliquoted into 96-well plates, with three parallel wells per sample. The optical density was measured at 570 nm using a microplate reader. The difference between the optical density of the well with ConA and the well without ConA represented the lymphocyte proliferation capacity. The results are shown in Table 2.

[0114] Table 2. Lymphocyte proliferation capacity of mice in each group ( ±s,n=10)

[0115]

[0116] As shown in Table 2, the lymphocyte proliferation capacity of Examples 1 to 7, Comparative Examples 1 and 2 was higher than that of the blank control group. The lymphocyte proliferation capacity of Examples 1 to 7 and Comparative Example 2 was significantly higher than that of the blank control group, indicating that the compositions obtained in Examples 1 to 7 and Comparative Example 2 have immune-enhancing effects. The lymphocyte proliferation capacity of Comparative Example 1 was lower than that of Examples 1 to 7 and Comparative Example 2. In Comparative Example 1, the raw material was reduced by removing *Bitter Thorn Flower*, indicating that *Bitter Thorn Flower* has a synergistic effect on enhancing immune function.

[0117] Experimental Example 3

[0118] One hundred and ten SPF-grade adult Balb / c mice (weighing 18–22 g) were randomly divided into 11 groups of 10 mice each: blank control group, model control group, groups of examples 1 to 7, comparative example 1, and comparative example 2. On day 1, mice in the model control group, Examples 1 to 7, Comparative Example 1, and Comparative Example 2 were subcutaneously injected with acetylphenylhydrazine (dose 20 mg / kg), while mice in the blank control group were subcutaneously injected with the same volume of physiological saline. Mice in Examples 1 to 7, Comparative Example 1, and Comparative Example 2 were gavaged with 0.1 g of the composition obtained in Examples 1 to 7, Comparative Example 1, and Comparative Example 2 (each composition was dissolved in 0.8 mL of sterile water before gavage). On days 2 and 3, mice in Examples 1 to 7, Comparative Example 1, and Comparative Example 2 were gavaged with 0.1 g of the composition obtained in Examples 1 to 7, Comparative Example 1, and Comparative Example 2 (each composition was dissolved in 0.8 mL of sterile water before gavage), once daily. On day 4, in the morning, mice in the model control group, Examples 1 to 7, Comparative Example 1, and Comparative Example 2 were subcutaneously injected with acetylphenylhydrazine (dose 40 mg / kg), and in the afternoon, they were intraperitoneally injected with cyclophosphamide (…). The mice in the control group, groups 1-7, comparative example 1, and comparative example 2 were administered 0.1 g / mouse of the compositions obtained in examples 1-7, comparative example 1, and comparative example 2 by gavage (each composition was dissolved in 0.8 mL of sterile water before gavage). From day 5 to day 7, the mice in the model control group, groups 1-7, comparative example 1, and comparative example 2 were injected intraperitoneally once daily with cyclophosphamide (dose 40 mg / kg). The mice in groups 1-7, comparative example 1, and comparative example 2 were administered 0.1 g / mouse of the compositions obtained in examples 1-7, comparative example 1, and comparative example 2 by gavage (each composition was dissolved in 0.8 mL of sterile water before gavage). On day 8, the vital signs of each group were observed and recorded, and blood was collected from the orbital sinus of each group of mice. Red blood cells (RBC), hemoglobin (HGB), white blood cells (WBC), and platelets (PLT) were detected using a fully automated blood cell analyzer. The results are shown in Table 3.

[0119] Table 3. Blood cell count in mice of each group ( ±s,n=10)

[0120]

[0121] As shown in Table 3, the levels of red blood cells (RBC), hemoglobin (HGB), white blood cells (WBC), and platelets (PLT) in groups 1 to 7, and comparative examples 1 and 2, were all higher than those in the model control group but lower than those in the blank control group. Groups 1 to 7 showed significantly higher levels than the model group, indicating that the compositions obtained in Examples 1 to 7 can effectively replenish blood in anemic mice after modeling. The levels of red blood cells (RBC), hemoglobin (HGB), white blood cells (WBC), and platelets (PLT) in comparative examples 1 and 2 were significantly lower than those in groups 1 to 7. In comparative example 1, the composition of the raw material was reduced by *Litsea cubeba*; in comparative example 2, the composition of the raw material was reduced by *Litsea cubeba*. This suggests that *Litsea cubeba* and *Litsea cubeba* have a synergistic effect in replenishing blood.

[0122] Test Example 4

[0123] One hundred and ten SD rats (90-110g) were randomly divided into a blank control group and the remaining rats were anesthetized with ether, and the scrotal skin was disinfected and both testes were removed. Postoperatively, penicillin 20000u / kg was injected intramuscularly twice daily for three consecutive days. The rats were then randomly divided into ten groups: a model control group, groups 1-7, comparative example 1, and comparative example 2. Rats in groups 1-7, comparative example 1, and comparative example 2 were administered 0.5g / rat via gavage once daily via groups gavage to each group (0.5g of each composition was dissolved in 1.5mL of sterile water before gavage). This continued for 30 days. Twenty-four hours after the last gavage, the rats were sacrificed, and the seminal vesicles, prostate, and levator ani muscle were rapidly harvested, accurately weighed, and organ indices were calculated. The results are shown in Table 4.

[0124] Table 4. Effects of each composition on the organ index of rat reproductive organs ( ±s,n=10)

[0125]

[0126] As shown in Table 4, the organ indices of the seminal vesicles and prostate gland in rats of Examples 1 to 7, and Comparative Examples 1 and 2, were significantly higher than those in the model control group but lower than those in the blank control group. The highest index was found in Example 4 group, reaching 535.21 mg / 100g bw, while the lowest was found in Comparative Example 2 group, at only 218.77 mg / 100g bw. Similarly, the organ indices of the levator ani muscle in rats of Examples 1 to 7, and Comparative Examples 1 and 2, were significantly higher than those in the model control group but lower than those in the blank control group. The highest index was found in Example 7 group, reaching 345.97 mg / 100g bw, while the lowest was found in Comparative Example 2 group, at only 142.17 mg / 100g bw. Therefore, the combinations obtained in Examples 1 to 7 have a significant effect on improving the organ indices of the seminal vesicles and prostate gland and the levator ani muscle in castrated rats, indicating that the compositions obtained in Examples 1 to 7 have a good kidney-tonifying effect.

[0127] The organ indices of seminal vesicle + prostate and levator ani muscle in Comparative Example 2 were significantly lower than those in Examples 1 to 7 and Comparative Example 1. In Comparative Example 2, the composition of Litsea cubeba was reduced. It can be seen that Litsea cubeba has a synergistic effect on improving the organ indices of seminal vesicle + prostate and levator ani muscle in castrated rats.

[0128] The embodiments of the present invention have been described in detail above, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and these variations still fall within the protection scope of the present invention.

Claims

1. A method for preparing a composition for replenishing qi and blood and tonifying the kidneys, characterized in that, Includes the following steps: S1. Weigh the following raw materials according to the following weight proportions: 5-25 parts of Panax notoginseng, 3-18 parts of Astragalus membranaceus, 10-20 parts of Lycium barbarum, 8-40 parts of Bitter Thorn Flower, 2-15 parts of Litsea cubeba, 5-18 parts of Gastrodia elata, 2-9 parts of Ginkgo biloba, and 3-12 parts of Dendrobium officinale; and pulverize each raw material separately. S2. Place the crushed Astragalus membranaceus, Lycium barbarum, Bitter Thorn Flower and Dendrobium officinale into a container, add water, decoct, filter, and obtain the first decoction and the first residue; put the first residue into a reflux extraction device, add 80-95% vol ethanol, heat and reflux for 2-4 hours, filter to obtain the second decoction and the second residue; distill the second decoction under reduced pressure, concentrate and dry, crush, and obtain the first extract; S3. Put the crushed Litsea cubeba, Gastrodia elata and Ginkgo biloba into a container, add water, decoct, filter to obtain the third decoction and the third residue, put the third residue into a reflux extraction device, add 50-75% vol ethanol, heat and reflux for 2-5 hours, filter to obtain the fourth decoction and the fourth residue; distill the fourth decoction under reduced pressure, concentrate and dry, crush to obtain the second extract. S4. Combine the first and third solutions, concentrate and dry them, then pulverize them to obtain the third extract. S5. Combine and mix the pulverized Panax notoginseng, the first extract, the second extract and the third extract evenly to obtain a composition that nourishes qi and blood and tonifies the kidneys.

2. The method for preparing the composition for replenishing qi and blood and tonifying the kidneys according to claim 1, characterized in that, In step S1, 2 to 5 parts of papaya were weighed and crushed.

3. The method for preparing the composition for replenishing qi and blood and tonifying the kidneys according to claim 2, characterized in that, The papaya is in 5 parts by weight.

4. The method for preparing the composition for replenishing qi and blood and tonifying the kidneys according to claim 3, characterized in that, The crushed papaya is processed in the same way as the raw material in step S3.

5. The method for preparing the composition for replenishing qi and blood and tonifying the kidneys according to claim 4, characterized in that, The ethanol concentration mentioned in step S2 is 90% vol.

6. The method for preparing the composition for replenishing qi and blood and tonifying the kidneys according to claim 5, characterized in that, The ethanol concentration mentioned in step S3 is 60% vol.

7. A composition for replenishing qi and blood and tonifying the kidneys, characterized in that, By weight, it consists of the following ingredients: 5-25 parts Panax notoginseng, 3-18 parts Astragalus membranaceus, 10-20 parts Lycium barbarum, 8-40 parts Bitter Thorn Flower, 2-15 parts Litsea cubeba, 5-18 parts Gastrodia elata, 2-9 parts Ginkgo biloba, and 3-12 parts Dendrobium officinale.

8. The composition for replenishing qi and blood and tonifying the kidneys according to claim 7, characterized in that, By weight, it consists of the following ingredients: 5-16 parts Panax notoginseng, 6-10 parts Astragalus membranaceus, 12-15 parts Lycium barbarum, 10-30 parts Bitter Thorn Flower, 5-8 parts Litsea cubeba, 8-15 parts Gastrodia elata, 3-5 parts Ginkgo biloba, and 5-10 parts Dendrobium officinale.

9. The composition for replenishing qi and blood and tonifying the kidneys according to claim 8, characterized in that, By weight, it consists of the following ingredients: 12 parts Panax notoginseng, 7 parts Astragalus membranaceus, 13 parts Lycium barbarum, 25 parts Bitter Thorn Flower, 7 parts Litsea cubeba, 11 parts Gastrodia elata, 3 parts Ginkgo biloba, and 7 parts Dendrobium officinale.

10. A composition for replenishing qi and blood and tonifying the kidneys, characterized in that, By weight, it consists of the following ingredients: 12 parts Panax notoginseng, 7 parts Astragalus membranaceus, 13 parts Lycium barbarum, 25 parts Bitter Thorn Flower, 7 parts Litsea cubeba, 11 parts Gastrodia elata, 3 parts Ginkgo biloba, 7 parts Dendrobium officinale, and 5 parts Chaenomeles speciosa.

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