A traditional Chinese medicine compound preparation with tranquilizing effect on cattle, and a preparation method and application thereof

Abstract

The application provides a traditional Chinese medicine compound preparation with a calming effect on cattle, a preparation method and application thereof, and relates to the technical field of traditional Chinese veterinary medicine. The traditional Chinese medicine compound preparation takes Litsea chenii, Spondias axillaris, Polygala, gold button, safflower, mace, clove as core raw materials, is supplemented with bright blue aluminum lake as an indicator, and is prepared by matching a dissolving agent, a thickening agent and deionized water. The traditional Chinese medicine compound preparation has a significant calming effect, and can play a targeted role on different cattle: for new cows, the milk yield can be effectively improved, and the incidence of postpartum diseases can be reduced; for bulls, the number of fighting behaviors can be significantly reduced, and the risk of injury can be reduced; for calves, the weaning stress response can be relieved, the number of weaning period bellowing can be reduced, and the incidence of diarrhea can be reduced. The traditional Chinese medicine compound preparation has the advantages of safety, high efficiency, convenient operation, no skin residue and the like, relies on visual marking to assist in drug administration management, provides a new solution for healthy breeding of large-scale cattle farms, and has a significant application value.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese veterinary medicine technology, specifically to a traditional Chinese medicine compound preparation with a calming and sedative effect on cattle, its preparation method, and its application. Background Technology

[0002] In large-scale cattle farming, cattle are susceptible to various stressors at different physiological stages and during the rearing process, leading to problems such as agitation and physiological dysfunction, which severely restrict farming efficiency and cattle health. Newly calved cattle experience a decline in immunity after calving, making them more vulnerable to stress, resulting in insufficient milk production and a high incidence of postpartum diseases. Bulls often engage in fighting due to herding behavior and environmental changes, causing physical injuries, disrupting the farming order, and increasing losses. Stress reactions during weaning in calves can cause diarrhea, frequent mooing, and other problems, affecting their growth and development. Currently available sedative products for cattle lack convenient administration methods and clear labeling to distinguish between treated and untreated cattle, making missed administration highly likely in actual farming.

[0003] To alleviate stress and achieve calming in cattle, current technologies mainly employ two approaches: chemical sedatives and traditional Chinese medicine preparations. Chemical sedatives, such as xylazine and detoxamide, while effective, have significant side effects, easily causing physiological disturbances in cattle such as respiratory depression, bradycardia, and abnormal blood pressure. They also tend to leave drug residues in the cattle's body, affecting the safety of livestock products. Traditional Chinese medicine preparations, with their advantages of safety and no residue, are gradually gaining attention in the field of cattle stress relief. They are typically administered via feed mixing, oral administration, or injection. Preparations such as astragalus polysaccharides can, to some extent, enhance cattle immunity and alleviate stress. However, existing Chinese medicine preparations have several shortcomings: First, their formulations lack specificity, often being broad-spectrum anti-stress formulas that cannot simultaneously meet the specific needs of cattle at different physiological stages, such as newborn calves, bulls, and calves, resulting in limited sedative and soothing effects. Second, their administration methods are inconvenient; feed mixing and oral administration require frequent contact with the cattle, which can exacerbate stress responses, while injection increases labor costs and operational difficulty. Therefore, developing a safe, efficient, targeted, and easy-to-use compound Chinese medicine preparation for cattle to address stress issues in cattle at different physiological stages and improve breeding efficiency has become an urgent need for the current large-scale cattle farming industry. Summary of the Invention

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a traditional Chinese medicine compound preparation with a calming and sedative effect on cattle, its preparation method, and its application.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] This invention provides a traditional Chinese medicine compound preparation with a calming and sedative effect on cattle. The traditional Chinese medicine compound preparation is composed of the following traditional Chinese medicine raw materials and excipients in parts by weight: the traditional Chinese medicine raw materials include 10-30 parts of Litsea cubeba, 5-15 parts of Ziziphus jujuba var. spinosa, 3-10 parts of Polygala tenuifolia, 3-15 parts of Clematis armandii, 5-20 parts of Carthamus tinctorius, 3-15 parts of Myristica fragrans, and 2-10 parts of Clove; the excipients include 0.1-0.5 parts of Brilliant Blue aluminum lake, 5-15 parts of propylene glycol, 2-5 parts of xanthan gum, and 80-120 parts of deionized water.

[0009] Preferably, the traditional Chinese medicine compound preparation is composed of the following traditional Chinese medicine raw materials and excipients in parts by weight: 20 parts of Litsea cubeba, 10 parts of Ziziphus jujuba var. spinosa, 5 parts of Polygala tenuifolia, 12 parts of Carthamus tinctorius, 8 parts of Myristica fragrans, 5 parts of Clove, 0.25 parts of Brilliant Blue Aluminum Lake, 10 parts of Propylene Glycol, 2.5 parts of Xanthan Gum, and 95 parts of Deionized Water.

[0010] Furthermore, the weight percentage of each fatty acid in the aforementioned traditional Chinese medicine compound preparation meets the following requirements: oleic acid ≥ 8.5%, linoleic acid ≥ 6.2%, palmitic acid ≥ 4.1%, lauric acid ≥ 2.3%, and myristic acid ≥ 1.8%.

[0011] Furthermore, the brilliant blue aluminum lake in the traditional Chinese medicine compound preparation is used as a blue indicator to distinguish between cattle that have been given the medication and those that have not, and it can be completely wiped away with water after 48 hours without leaving any skin residue.

[0012] This invention provides a method for preparing a traditional Chinese medicine compound preparation with a calming and sedative effect on cattle, comprising the following steps:

[0013] (1) Raw material pretreatment: The Litsea cubeba, Ziziphus jujuba var. spinosa, Polygala tenuifolia, Carthamus tinctorius, Myristica fragrans, and Cloves were pulverized and passed through a 40-80 mesh sieve, and the moisture content was controlled to be ≤8%;

[0014] (2) Extraction: Mix the raw materials by weight, add 8-12 times the amount of 70%-95% ethanol solution, reflux at 75-85℃ for 2-3 times, each time for 1.5-2.5 h, combine the extracts, and concentrate under reduced pressure at 50-60℃ and -0.06~-0.08 MPa until there is no alcohol smell, to obtain a thick paste of Chinese herbal extract;

[0015] (3) Formulation preparation: Mix the above thick paste with propylene glycol to dissolve, add xanthan gum and stir until uniform, then add brilliant blue aluminum lake and deionized water to make up the volume, ultrasonically disperse at 300-500 W power for 15-30 min, filter through a 0.22 μm filter membrane to obtain a spray-type composite formulation;

[0016] (4) Packaging: The obtained preparation is filled into a metered spray bottle and sealed to obtain the finished product.

[0017] Preferably, in step (1), the raw material is crushed and passed through a 60-mesh sieve, and the water content is controlled to be 5%-7%; in step (2), the volume fraction of the ethanol solution is 85%, the amount added is 10 times the total weight of the raw material, the reflux extraction temperature is 80℃, the extraction is performed twice, each time for 2 hours, the vacuum concentration temperature is 55℃, and the pressure is -0.07MPa; in step (3), the ultrasonic dispersion power is 400W, and the dispersion time is 20min.

[0018] Furthermore, the viscosity of the spray formulation is 60-80 mPa·s (measured at 25°C), and the spray particle size is 80-120 μm.

[0019] This invention provides the application of the aforementioned traditional Chinese medicine compound preparation in the preparation of calming and sedative products for cattle.

[0020] Furthermore, the method of administration for the aforementioned traditional Chinese medicine compound preparation is to spray it onto the skin of the forehead between the two horns of the cow (as shown in the attached image). Figure 1 As shown in the figure, the spraying dosage is 0.5-2 mL / head / time, 1-2 times daily.

[0021] Furthermore, the recommended spraying dosage for different types of cattle is as follows: 1.5-2 mL / head / time for bulls, 0.5-1 mL / head / time for calves, and 1-1.5 mL / head / time for dairy cows.

[0022] Furthermore, the traditional Chinese medicine compound preparation is used to alleviate stress response in cattle, including reducing bull fighting behavior, reducing the number of mooings and the incidence of diarrhea in calves during weaning, increasing milk production in newly calved cows, and reducing the incidence of postpartum diseases.

[0023] The pharmacological effects of each Chinese herbal raw material in the compound preparation of this invention are as follows:

[0024] Litsea cubeba: The fruit of *Litsea cubeba*, *Litsea pubescens*, and *Litsea spp.*, all belonging to the Lauraceae family. It has a pungent taste and warm nature, and enters the spleen and stomach meridians. It has the effects of warming the middle jiao (spleen and stomach), promoting qi circulation and relieving pain, drying dampness, strengthening the spleen and promoting digestion, and detoxifying and reducing swelling. It is used for stomach cold and abdominal pain, summer heat and dampness causing vomiting and diarrhea, food stagnation and bloating, dysmenorrhea, hernia pain, malaria, and sores and swelling.

[0025] Sour jujube seed: the dried, mature seed of the sour jujube plant (Rhamnaceae family). It has a sweet and sour taste, is neutral in nature, and enters the heart, liver, and gallbladder meridians. It nourishes the heart and liver, calms the mind and soothes the nerves, astringes sweat, and promotes the production of body fluids. Its core function is sedation and hypnosis, inhibiting excessive excitation of the central nervous system. It is suitable for treating agitation, restlessness, and palpitations caused by stress in cattle, effectively relieving emotional tension and enhancing sedation and soothing effects. It is also non-toxic and safe for long-term use.

[0026] Polygala tenuifolia: The dried root of Polygala tenuifolia or Polygala ovoidea, plants of the Polygalaceae family. It tastes bitter and pungent, is warm in nature, and enters the heart, kidney, and lung meridians. It has the effects of calming the mind and soothing the nerves, resolving phlegm and opening the orifices, and dissipating carbuncles and swellings. It can enhance the sedative and calming effect of Ziziphus jujuba var. spinosa, while also assisting in regulating the gastrointestinal function of cattle, improving digestive disorders under stress, and forming a synergistic effect when combined with Ziziphus jujuba var. spinosa, thus enhancing the overall calming and sedative effect. It has high tolerance in ruminants.

[0027] Golden Button: The whole herb of *Asteris chinensis*, a plant in the Asteraceae family. It has a pungent taste and warm properties; it has detoxifying, diuretic, cough-relieving, asthma-relieving, swelling-reducing, and pain-relieving effects. It is used for malaria, toothache, enteritis, dysentery, cough, asthma, whooping cough, and tuberculosis. Externally, it is used to treat snake bites, dog bites, and boils and carbuncles.

[0028] Safflower: The dried flower of *Carthamus tinctorius*, a plant in the Asteraceae family. It has a pungent taste and warm properties, and enters the heart and liver meridians; it has the effects of promoting blood circulation, regulating menstruation, dispersing blood stasis, and relieving pain. It is used for amenorrhea, dysmenorrhea, lochia retention, abdominal masses, chest pain, abdominal pain due to blood stasis, stabbing pain in the chest and ribs, injuries from falls, and sores and swellings.

[0029] Nutmeg: The dried seed kernel of the nutmeg plant (Myristica fragrans). It has a pungent taste and warm properties, and enters the spleen, stomach, and large intestine meridians; it has the effects of warming the middle jiao (spleen and stomach), promoting qi circulation, astringing the intestines, and stopping diarrhea. It is used for spleen and stomach deficiency-cold, chronic diarrhea, abdominal distension and pain, poor appetite, and vomiting.

[0030] Clove: The dried flower buds of the clove tree, a plant in the Myrtaceae family. It has a pungent taste and warm properties, and enters the spleen, stomach, lung, and kidney meridians; it has the effects of warming the middle jiao and relieving nausea, tonifying the kidneys and assisting yang. It is used for spleen and stomach deficiency-cold, hiccups and vomiting, poor appetite and diarrhea, cold pain in the heart and abdomen, and kidney deficiency and impotence.

[0031] (III) Beneficial Effects

[0032] This invention provides a traditional Chinese medicine compound preparation with a calming and sedative effect on cattle, its preparation method, and its application. The compound preparation uses Litsea cubeba, Ziziphus jujuba var. spinosa, Polygala tenuifolia, Clematis armandii, Carthamus tinctorius, Myristica fragrans, and Syzygium aromaticum as core medicinal ingredients, supplemented with brilliant blue aluminum lake as an indicator, and prepared with propylene glycol as a solubilizer, xanthan gum as a thickener, and deionized water. This invention formulates the preparation as a spray, effectively avoiding the additional stress caused by frequent contact with cattle due to traditional drug administration methods such as mixing with feed or oral administration, while significantly reducing labor input and operational difficulty. The visual mark formed by the brilliant blue aluminum lake can be maintained for more than 24 hours and can be completely wiped away with water, leaving no skin residue and causing no irritation. This helps farmers accurately distinguish the drug administration status of cattle, effectively avoiding missed or accidental spraying and improving the efficiency of livestock management.

[0033] This invention's herbal compound preparation exhibits significant sedative and soothing effects, and can be specifically targeted at different types of cattle: for newly calved cattle, it can effectively increase milk production and reduce the incidence of postpartum diseases; for bulls, it can significantly reduce fighting and lower the risk of injury; for calves, it can alleviate weaning stress, reduce mooing during weaning, and lower the incidence of diarrhea. This herbal compound preparation also boasts advantages such as safety, high efficiency, ease of use, and no skin residue. Utilizing visual markers to assist in drug administration management, it provides a new solution for healthy cattle farming in large-scale operations, demonstrating significant application value. Attached Figure Description

[0034] Figure 1 This diagram shows the application site on the forehead of a cow, clearly indicating that the spraying area for the formulation is the forehead between the cow's two horns. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] Example 1

[0037] A traditional Chinese medicine compound preparation with a calming and sedative effect on cattle, comprising the following raw materials in parts by weight: 20 parts Litsea cubeba, 10 parts Ziziphus jujuba var. spinosa, 5 parts Polygala tenuifolia, 8 parts Clematis armandii, 12 parts Carthamus tinctorius, 8 parts Myristica fragrans, and 5 parts Clove; excipients include 0.25 parts Brilliant Blue aluminum lake, 10 parts propylene glycol, 2.5 parts xanthan gum, and 95 parts deionized water. The preparation method of the traditional Chinese medicine compound preparation includes the following steps:

[0038] (1) Raw material pretreatment: Litsea cubeba, Ziziphus jujuba var. spinosa, Polygala tenuifolia, Clematis armandii, Carthamus tinctorius, Myristica fragrans, and Cloves were pulverized and passed through a 60-mesh sieve, and the moisture content was controlled to be 6%;

[0039] (2) Extraction: Mix the pretreated raw materials according to the ratio, add 10 times the amount of 85% ethanol solution, reflux at 80℃ twice, 2 h each time, combine the extracts, and concentrate under reduced pressure at 55℃ and -0.07 MPa until there is no alcohol taste to obtain a thick paste of Chinese herbal extract.

[0040] (3) Formulation preparation: Mix the thick paste with propylene glycol and stir until completely dissolved. Add xanthan gum and continue stirring until the system is homogeneous. Then add brilliant blue aluminum lake and deionized water to make up the volume. Use 400 W power to ultrasonically disperse for 20 min and filter through a 0.22 μm filter membrane.

[0041] (4) Packaging: The filtered preparation is filled into 100 mL quantitative spray bottles and sealed to obtain the finished product. The prepared preparation contains 9.5% oleic acid, 7.2% linoleic acid, 4.7% palmitic acid, 3.0% lauric acid, and 2.3% myristic acid, with a viscosity of 70 mPa·s (25℃) and a spray particle size of 100 μm.

[0042] Example 2

[0043] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 10 parts of Litsea cubeba, 5 parts of Ziziphus jujuba var. spinosa, 3 parts of Polygala tenuifolia, 3 parts of Clematis armandii, 5 parts of Carthamus tinctorius, 3 parts of Myristica fragrans, and 2 parts of Clove; the excipients include 0.10 parts of Brilliant Blue Aluminum Lake, 5 parts of Propylene Glycol, 2 parts of Xanthan Gum, and 80 parts of Deionized Water.

[0044] Example 3

[0045] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 25 parts of Litsea cubeba, 13 parts of Ziziphus jujuba var. spinosa, 8 parts of Polygala tenuifolia, 12 parts of Clematis armandii, 15 parts of Carthamus tinctorius, 12 parts of Myristica fragrans, and 8 parts of Clove; the excipients include 0.42 parts of Brilliant Blue Aluminum Lake, 12 parts of Propylene Glycol, 4.5 parts of Xanthan Gum, and 110 parts of Deionized Water.

[0046] Example 4

[0047] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 12 parts of Litsea cubeba, 6 parts of Ziziphus jujuba var. spinosa, 4 parts of Polygala tenuifolia, 5 parts of Clematis armandii, 7 parts of Carthamus tinctorius, 5 parts of Myristica fragrans, and 4 parts of Clove; the excipients include 0.15 parts of Brilliant Blue Aluminum Lake, 6 parts of Propylene Glycol, 2.2 parts of Xanthan Gum, and 85 parts of Deionized Water.

[0048] Example 5

[0049] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 30 parts of Litsea cubeba, 15 parts of Ziziphus jujuba var. spinosa, 10 parts of Polygala tenuifolia, 15 parts of Clematis armandii, 20 parts of Carthamus tinctorius, 15 parts of Myristica fragrans, and 10 parts of Clove; the excipients include 0.50 parts of Brilliant Blue Aluminum Lake, 15 parts of Propylene Glycol, 5 parts of Xanthan Gum, and 120 parts of Deionized Water.

[0050] Example 6

[0051] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 22 parts of Litsea cubeba, 12 parts of Ziziphus jujuba var. spinosa, 6 parts of Polygala tenuifolia, 9 parts of Clematis armandii, 18 parts of Carthamus tinctorius, 9 parts of Myristica fragrans, and 7 parts of Clove; the excipients include 0.30 parts of Brilliant Blue Aluminum Lake, 10 parts of Propylene Glycol, 3.5 parts of Xanthan Gum, and 100 parts of Deionized Water.

[0052] Example 7

[0053] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 15 parts of Litsea cubeba, 8 parts of Ziziphus jujuba var. spinosa, 6 parts of Polygala tenuifolia, 6 parts of Clematis armandii, 9 parts of Carthamus tinctorius, 6 parts of Myristica fragrans, and 5 parts of Clove; the excipients include 0.20 parts of Brilliant Blue Aluminum Lake, 8 parts of Propylene Glycol, 3.2 parts of Xanthan Gum, and 95 parts of Deionized Water.

[0054] Comparative Example 1

[0055] The difference between this comparative example and Example 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 10 parts of jujube seed, 5 parts of polygala root, 12 parts of safflower, 8 parts of nutmeg, and 5 parts of clove; the excipients include 0.25 parts of brilliant blue aluminum lake, 10 parts of propylene glycol, 2.5 parts of xanthan gum, and 95 parts of deionized water.

[0056] Comparative Example 2

[0057] The difference between this comparative example and Example 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 20 parts of Litsea cubeba, 8 parts of golden button, 12 parts of safflower, and 5 parts of clove; the excipients include 0.25 parts of brilliant blue aluminum lake, 10 parts of propylene glycol, 2.5 parts of xanthan gum, and 95 parts of deionized water.

[0058] Comparative Example 3

[0059] The difference between this comparative example and Example 1 is that the traditional Chinese medicine compound preparation is made from the following raw materials in parts by weight: 20 parts of Litsea cubeba, 10 parts of Ziziphus jujuba var. spinosa, 5 parts of Polygala tenuifolia, and 8 parts of Myristica fragrans; the excipients include 0.25 parts of Brilliant Blue Aluminum Lake, 10 parts of Propylene Glycol, 2.5 parts of Xanthan Gum, and 95 parts of Deionized Water.

[0060] Experimental Example 1

[0061] The effects of the traditional Chinese medicine compound preparation of this invention on milk yield and postpartum disease incidence in newly calved cows

[0062] 1. Test Methods

[0063] One hundred and fifty healthy newborn Holstein dairy cows of similar parity, weight, and milk production performance were randomly divided into five groups of 30 cows each: a control group and experimental groups 1-4. Experimental group 1 was sprayed with the traditional Chinese medicine compound preparation prepared according to Example 1; experimental groups 2-4 were sprayed with the traditional Chinese medicine compound preparations prepared according to Comparative Examples 1-3; and the control group was sprayed with an equal volume of deionized water. The application site was the skin on the forehead between the cow's horns (e.g., [missing information]). Figure 1(As shown), administer 1.2 mL / head once daily for 7 consecutive days. Record the milk yield of each group of cattle daily and calculate the average milk yield over 7 days; statistically analyze the occurrence of postpartum diseases (including metritis, mastitis, postpartum paralysis, etc.) during the trial period and calculate the postpartum morbidity rate. Morbidity rate = (number of sick cattle / total number of cattle in the group) × 100%.

[0064] 2. Test Results

[0065] Table 1 shows that the average milk yield of newborn cows in the control group was 12.45 ± 1.08 kg in 7 days, and the postpartum morbidity rate was 16.7%. The average milk yield of newborn cows in experimental groups 1-4 was significantly higher than that of the control group in 7 days, and the postpartum morbidity rate was significantly lower. Among them, experimental group 1 showed the most outstanding improvement in milk yield and disease control, with an average milk yield 29.90% higher than that of the control group in 7 days, and a postpartum morbidity rate only 1 / 5 of that of the control group. This indicates that the synergistic effect among the various Chinese herbal raw materials in the compound preparation of this invention significantly enhances its calming and sedative effects, thereby improving the physiological state of newborn cows.

[0066] Table 1. Comparison of average milk yield and postpartum morbidity in each group of newborn cows.

[0067]

[0068] Note: Compared with the control group, * P<0.05, ** P<0.01.

[0069] Experimental Example 2

[0070] The effect of the traditional Chinese medicine compound preparation of this invention on bull fighting behavior

[0071] 1. Test Methods

[0072] Sixty healthy Simmental bulls, weighing (500±20) kg and free from limb injuries, infectious diseases, and behavioral abnormalities, were randomly divided into a control group, an experimental group, and a positive control group, with 20 bulls in each group. The three groups of bulls were housed in separate pens (10 bulls per pen). The experimental group was sprayed with the traditional Chinese medicine compound preparation prepared according to Example 1. The positive control group was sprayed with a commercially available conventional bovine sedative traditional Chinese medicine preparation (main ingredient: jujube seed extract, dosage 1.5 mL / head / time as recommended in the instructions). The control group was sprayed with an equal volume of deionized water. The application site was the skin on the forehead between the horns (e.g.,...). Figure 1As shown in the image, the administration was once daily, 2 mL / head each time (the positive control group was adjusted to 1.5 mL / head per dose according to the instructions), for 30 consecutive days. During the administration period, two observers simultaneously monitored the bulls in each group daily, recording the number of fights (one fight was defined as limb collisions or fighting between two or more bulls lasting ≥3 seconds) and injuries such as skin breaks and limb swelling, and calculating the injury rate. Injury rate = (number of injured bulls / total number of bulls in the group) × 100%.

[0073] 2. Test Results

[0074] Table 2 shows that the average number of fights per day for bulls in the control group was 8.28±1.13, with an injury rate of 46.7%; the average number of fights per day for bulls in the positive control group was 4.52±0.89, with an injury rate of 23.3%; and the average number of fights per day for bulls in the experimental group was 2.97±0.71, with an injury rate of 10.36±2.82%. Compared with the control group, the number of fights and the injury rate in both the positive control group and the experimental group were significantly reduced (P<0.05). Compared with the positive control group, the number of fights in the experimental group decreased by 34.29%, and the injury rate decreased by 55.54%, with statistically significant differences (P<0.05). This indicates that the sedative effect of the herbal compound preparation of this invention is superior to that of commercially available conventional sedative Chinese medicines, and it can more significantly inhibit bull fighting behavior and reduce the risk of bodily injury.

[0075] Table 2 Comparison of average daily number of fights and injury rate among bulls in each group

[0076]

[0077] Note: Compared with the control group, * P<0.05, ** P<0.01; compared with the positive control group, # P<0.05.

[0078] Experimental Example 3

[0079] The effect of the traditional Chinese medicine compound preparation of this invention on weaning stress in calves

[0080] 1. Test Methods

[0081] Eighty healthy Holstein calves, unweaned and without a history of diarrhea or infectious diseases, aged (60±5 days), were randomly divided into a control group, an experimental group, and a positive control group, with 40 calves in each group. The three groups of calves were housed in separate pens (10 calves per pen) with identical pens conditions and a uniform weaning program (gradually reducing the amount of breast milk starting on day 1 and completely weaning on day 3). After weaning, they were fed the same calf feed. The experimental group was sprayed with a traditional Chinese medicine compound preparation prepared according to Example 1, the positive control group was sprayed with a commercially available conventional bovine sedative traditional Chinese medicine preparation (main ingredient: jujube seed extract, dosage according to the recommended dose of 0.6 mL / calves / time), and the control group was sprayed with an equal volume of deionized water. The application site was the skin on the forehead between the horns (e.g., Figure 1 As shown in the diagram, administer 0.8 mL / cattle twice daily (0.6 mL / cattle per dose for the positive control group, adjusted according to the instructions), for 20 consecutive days. During the experiment, record the number of mooings and diarrhea status of calves in each group daily (diarrhea is defined as loose or watery feces, with ≥3 defecations per day), and calculate the diarrhea incidence rate. Diarrhea incidence rate = (number of cattle with diarrhea / total number of cattle in the group) × 100%.

[0082] 2. Test Results

[0083] Table 3 shows that the average daily mooing frequency of calves in the control group was 52.44±9.30 times, with a diarrhea incidence rate of 37.5%; the average daily mooing frequency of calves in the positive control group was 30.12±7.15 times, with a diarrhea incidence rate of 25.0%; and the average daily mooing frequency of calves in the experimental group was 18.50±6.06 times, with a diarrhea incidence rate of 15.0%. Compared with the control group, the average daily mooing frequency and diarrhea incidence rate of both the positive control group and the experimental group were significantly reduced (P<0.05). Compared with the positive control group, the average daily mooing frequency of the experimental group decreased by 38.58%, and the diarrhea incidence rate decreased by 40.0%, with statistically significant differences (P<0.05). This indicates that the preparation of this invention is more effective than commercially available conventional sedative Chinese medicines in relieving weaning stress in calves, and can more effectively improve the physiological state during weaning, reduce the number of moos, and lower the diarrhea incidence rate.

[0084] Table 3 Comparison of average daily mooing frequency and diarrhea incidence in calves of different groups

[0085]

[0086] Note: Compared with the control group, * P<0.05, ** P<0.01; compared with the positive control group, # P<0.05.

[0087] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine compound preparation with a calming and sedative effect on cattle, characterized in that, The traditional Chinese medicine compound preparation is composed of the following traditional Chinese medicine raw materials and excipients in parts by weight: the traditional Chinese medicine raw materials include 10-30 parts of Litsea cubeba, 5-15 parts of Ziziphus jujuba var. spinosa, 3-10 parts of Polygala tenuifolia, 3-15 parts of Clematis armandii, 5-20 parts of Carthamus tinctorius, 3-15 parts of Myristica fragrans, and 2-10 parts of Clove; the excipients include 0.1-0.5 parts of Brilliant Blue Aluminum Lake, 5-15 parts of Propylene Glycol, 2-5 parts of Xanthan Gum, and 80-120 parts of Deionized Water; the Brilliant Blue Aluminum Lake is used as a blue indicator to form an erasable blue mark on the skin of the cow's forehead.

2. The traditional Chinese medicine compound preparation according to claim 1, characterized in that, The traditional Chinese medicine compound preparation is composed of the following traditional Chinese medicine raw materials and excipients in parts by weight: 20 parts of Litsea cubeba, 10 parts of Ziziphus jujuba var. spinosa, 5 parts of Polygala tenuifolia, 8 parts of Clematis armandii, 12 parts of Carthamus tinctorius, 8 parts of Myristica fragrans, 5 parts of Clove, 0.25 parts of Brilliant Blue Aluminum Lake, 10 parts of Propylene Glycol, 2.5 parts of Xanthan Gum, and 95 parts of Deionized Water.

3. The method for preparing the traditional Chinese medicine compound preparation according to any one of claims 1-2, characterized in that, The preparation method of the spray of the traditional Chinese medicine compound preparation includes the following steps: (1) Raw material pretreatment: The Litsea cubeba, Ziziphus jujuba var. spinosa, Polygala tenuifolia, Clematis armandii, Carthamus tinctorius, Myristica fragrans, and Cloves were pulverized and passed through a 40-80 mesh sieve, and the moisture content was controlled to be ≤8%; (2) Extraction: Mix the raw materials by weight, add 8-12 times the amount of 70%-95% ethanol solution, reflux at 75-85℃ for 2-3 times, each time for 1.5-2.5h, combine the extracts, and concentrate under reduced pressure at 50-60℃ and -0.06~-0.08MPa until there is no alcohol smell, to obtain a thick paste of Chinese herbal extract; (3) Formulation preparation: Mix the above thick paste with propylene glycol to dissolve, add xanthan gum and stir until uniform, then add brilliant blue aluminum lake and deionized water to make up the volume, ultrasonically disperse at 300-500W power for 15-30min, filter through a 0.22μm filter membrane to obtain a spray-type composite formulation; (4) Packaging: The obtained preparation is filled into a metered spray bottle and sealed to obtain the finished product.

4. The method for preparing the traditional Chinese medicine compound preparation according to claim 3, characterized in that, The viscosity of the spray formulation is 60-80 mPa·s (measured at 25℃), and the spray particle size is 80-120 μm.

5. The application of a traditional Chinese medicine compound preparation as described in any one of claims 1-3 in the preparation of bovine calming and sedative products.

6. The application according to claim 5, characterized in that, The dosage of the herbal compound preparation spray is 0.5-2 mL / head / time, 1-2 times daily.

7. The application according to claim 5, characterized in that, The recommended spraying dosage for different types of cattle is as follows: 1.5-2 mL / head / time for bulls, 0.5-1 mL / head / time for calves, and 1-1.5 mL / head / time for dairy cows.

Images