A shower gel based on synergistic repair of composite functional peptides and a preparation method thereof

Abstract

The application provides an anti-stay-up body care shower gel based on synergistic repair of composite functional peptides and a preparation method thereof. The shower gel comprises peptide-carrying composite microcapsules, TEA-coconut oil amido glutamate, cocamidopropyl betaine, fragrance components, repair aids, solubilizers and deionized water, and the pH value of the finished product is accurately adjusted to 5.5-6.0. By introducing cationic microcapsules with high positive charge density into an amino acid surfactant matrix, the active polypeptide can be effectively deposited and attached to the negatively charged skin surface during the rinsing process by using electrostatic attraction, thereby solving the problem of low retention rate of active ingredients. The polypeptide composition comprises a first peptide, a second peptide and a third peptide, which respectively act on muscle tissue, the dermis layer and the epidermis layer. The preparation process adopts a segmented temperature control mode, thereby ensuring the stability of biological activity. The application can significantly relieve muscle soreness, barrier damage and dull skin caused by staying up late, and assist in improving sleep.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, specifically to a shower gel based on the synergistic repair of compound functional peptides and its preparation method. Background Technology

[0002] With the fast pace of modern life, staying up late has become a common phenomenon. Staying up late not only causes fatigue in the central nervous system, but also triggers a series of physiological chain reactions, mainly manifested as persistent muscle tension and stiffness, damage to the skin barrier, and dull skin due to increased oxidative stress. In the field of daily chemical cleaning products, existing body care products mostly focus on basic cleaning and simple surface moisturizing, which are difficult to provide substantial repair for the above-mentioned comprehensive symptoms caused by staying up late.

[0003] Currently, although there have been attempts to add active ingredients such as peptides to bath products to enhance their efficacy, there are significant technical limitations in practical applications. First, most peptide active substances have high water solubility, and in rinse-off cleansing products, these ingredients are easily lost with large amounts of water and the rinsing action of surfactants, making it difficult to retain them effectively on the skin surface. Second, the anionic surfactant systems used in conventional shower gels often interfere with the spatial structure of peptide molecules, causing their biological activity to degrade during storage or use. In addition, existing anti-fatigue products often rely solely on the addition of essential oils to achieve scent regulation, lacking a delivery system that can penetrate and act on muscle tissue and the dermis, resulting in their repair effects remaining only on the superficial surface of the skin and failing to address the deep physiological damage caused by staying up late. Therefore, developing a bath product that can improve the retention rate of active peptides in a rinsing environment and achieve multi-target synergistic repair is the current research focus in this field. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a shower gel based on the synergistic repair of complex functional peptides and its preparation method, solving the comprehensive physiological reactions caused by prolonged sleep deprivation, such as muscle stiffness and soreness, damaged skin barrier, dull skin tone, and sleep disorders. Existing rinse-off body cleansing products struggle to effectively retain active repair ingredients during the washing process. Water-soluble active substances such as peptides are easily deactivated in conventional surfactant systems and have a high loss rate under water rinsing, making it difficult to achieve substantial skin repair and muscle relaxation.

[0005] To address the above problems, the present invention provides the following technical solution: In a first aspect, the present invention provides an anti-late-night body care shower gel based on the synergistic repair of compound functional peptides, employing the following technical solution: A body care shower gel based on the synergistic repair of complex functional peptides for combating sleep deprivation comprises the following components by weight percentage: peptide-loaded complex microcapsules 0.5%–3.0%; cocoyl glutamic acid TEA salt 12.0%–20.0%; cocamidopropyl betaine 3.0%–8.0%; aromatic components 0.5%–2.0%; repairing aids 0.2%–1.5%; solubilizer 0–2.0%; citric acid, the amount of which makes the pH of the shower gel 5.5–6.0; and deionized water as the balance.

[0006] By adopting the above technical solution, this invention introduces peptide-loaded composite microcapsules into the shower gel system, solving the problem of low retention rate of peptide ingredients in rinse-off products. The isoelectric point of the stratum corneum of human skin is between pH 4.2 and 5.0, and under normal bathing conditions, the skin surface carries a negative charge. The outer layer of the peptide-loaded composite microcapsules is composed of cationic polymers with a high density of positive charges. During shower application and subsequent rinsing, the positive charge on the microcapsule surface and the negative charge on the skin surface generate electrostatic attraction. The strength of this electrostatic adsorption is greater than the mechanical shear force generated by the water flow and the eluting force of the surfactant, allowing the peptide-encapsulated microcapsules to settle and adhere to the surface of the stratum corneum. The system uses cocoyl glutamate (TEA) salt and cocamidopropyl betaine to construct the cleaning matrix. This combination is an isoelectric point-slightly acidic surfactant system that will not damage the microcapsule structure. Controlling the pH of the finished product between 5.5 and 6.0 maintains the high charge density of the polymer, ensuring the occurrence of electrostatic adsorption, while also conforming to the physiological microenvironment of the skin surface. After the microcapsules adhere to the skin surface, they can continuously release the internal peptide composition during the post-washing stage. Combined with repairing agents and aromatic components, they can achieve synergistic regulation of skin barrier repair, muscle relaxation and nerve calming.

[0007] Preferably, the peptide-loaded composite microcapsules are made from raw materials comprising the following parts by weight: 1 part of a polypeptide composition; 0.5-1.2 parts of a cationic polymer; 0.1-0.5 parts of hydrogenated phospholipids; and 0-1.0 parts of hydroxypropyl methylcellulose. The polypeptide composition consists of a first peptide, a second peptide, and a third peptide, and the weight ratio of the first peptide, the second peptide, and the third peptide is 3:3:4. The first peptide is selected from one or a combination of two of acetyl hexapeptide-8 and dipeptide diaminobutyryl benzylamide diacetate; the second peptide is selected from one or a combination of two of palmitoyl tripeptide-5 and copper tripeptide-1; and the third peptide is selected from one or a combination of two of glutathione and carnosine. The cationic polymer is selected from one or a combination of two of polyquaternium-7 and polyquaternium-10.

[0008] By employing the above technical solution, cationic polymers serve as the framework material for microcapsules, providing the charge required for electrostatic adsorption; hydrogenated phospholipids form a lipid bilayer structure around the microcapsules, enhancing their compatibility with the human skin's lipid membrane; hydroxypropyl methylcellulose improves the physical stability of the microcapsules in the aqueous detergent system through steric hindrance, preventing aggregation and flocculation. The three peptides, combined in a specific ratio, have the following mechanism of action: the first peptide competitively binds to protein receptors at the neuromuscular junction, blocking the release of acetylcholine neurotransmitters, reducing the frequency of local muscle contractions, and relieving muscle stiffness and soreness; the second peptide activates transforming growth factor in skin fibroblasts, promoting the synthesis of endogenous collagen and elastin, and accelerating the reconstruction of the damaged skin barrier; the third peptide, through thiol reactions and anti-glycation effects, eliminates reactive oxygen free radicals generated by oxidative stress in skin tissue during periods of sleep deprivation, reducing melanin deposition. The combination of these three components achieves repair of muscle tissue, the dermis, and the epidermis.

[0009] Preferably, the aromatic component is selected from one or more combinations of lavender essential oil, bergamot essential oil, vetiver essential oil, and valerian extract; the solubilizer is selected from one or a combination of two of polysorbate-20 and PEG-40 hydrogenated castor oil. The repairing aids include ceramide NP, sodium hyaluronate, bisabolol, and panthenol.

[0010] By employing the above technical solutions, the volatile small molecules contained in the aromatic components act on the limbic system of the human central nervous system through the olfactory nerve pathway, reducing the concentration of cortisol in the body and producing a sedative effect to shorten the sleep latency. The solubilizer can uniformly disperse the lipid-soluble aromatic components in the aqueous system to form a transparent microemulsion, preventing product stratification. The repairing agent replenishes the lack of intercellular lipids in the stratum corneum, enhances epidermal hydration, and inhibits the release of inflammatory factors.

[0011] Secondly, this invention provides a method for preparing an anti-late-night body care shower gel based on the synergistic repair of compound functional peptides, using the following technical solution: A method for preparing an anti-stay-up-night body care shower gel based on the synergistic repair of compound functional peptides includes the following steps: (1) Matrix pretreatment: heating deionized water to 70℃~75℃, adding cocoyl glutamic acid TEA salt, cocamidopropyl betaine and ceramide NP in the repair aid, stirring at 100~200RPM until the solid is completely dissolved to form a uniform base liquid; (2) Cooling stage: lowering the temperature of the uniform base liquid to 35℃~40℃; (3) Core component addition: adding peptide-carrying compound microcapsules to the uniform base liquid, and adding the remaining components of the repair aid except ceramide NP, stirring at 50~100RPM for 15~30 minutes to ensure uniform dispersion; (4) Fragrance system introduction: after premixing the fragrance components and solubilizer to form a mixture, adding the mixture to the main tank and stirring evenly; (5) Finished product adjustment: adjusting the pH value of the system to 5.5~6.0 with citric acid, letting it stand to defoam, and obtaining the finished product.

[0012] By employing the above technical solution, the preparation process involves segmented treatment of heat-resistant high-melting-point substances and heat-sensitive active substances. Surfactants and high-melting-point ceramide NPs are treated within the 70℃ to 75℃ range, ensuring complete dissolution to form a homogeneous and stable base solution. After a cooling stage to control the system temperature below 40℃, peptide-loaded composite microcapsules and other water-soluble active substances are added. This temperature control avoids peptide bond breakage, loss of peptide activity, and thermodynamic deformation of the microcapsule's outer phospholipid membrane caused by high temperatures, thus guaranteeing the product's efficacy stability and physical structural integrity.

[0013] Preferably, before step (3), the peptide-loaded composite microcapsules are pre-prepared as follows: (a) Aqueous phase preparation: The peptide composition is dissolved in deionized water to obtain a peptide premix; (b) Charge adjustment: Under stirring, the pH value of the peptide premix is ​​adjusted to 4.5-5.5 using a 5% citric acid solution; (c) Self-assembly into nuclei: A cationic polymer solution is added dropwise to the peptide premix after pH adjustment to perform electrostatic self-assembly and form a primary composite micronucleus; (d) Microcapsule stabilization: Hydrogenated phospholipids and hydroxypropyl methylcellulose are added to the system containing the primary composite micronucleus, and stirring is continued to form a mixture containing microcapsules; (e) Homogenization: The mixture containing microcapsules is placed in a high-pressure homogenizer and circulated 2-3 times under a pressure of 20-40 MPa to obtain a peptide-loaded composite microcapsule suspension with an average microcapsule particle size of 260 nm-780 nm.

[0014] By employing the above technical solution, the microcapsules are constructed based on the electrostatic complexation and aggregation of polymers. In step (b), the pH of the peptide solution is adjusted to 4.5 to 5.5, which is higher than the isoelectric point of most amino acid residues in the peptide composition, promoting dissociation of the peptide molecule surface and exposing anionic charge sites. In step (c), a polyquaternary ammonium salt with a high positive charge density is added. The positively charged polymer chains and negatively charged peptide molecules approach each other through electrostatic attraction and undergo charge neutralization, inducing phase separation in the system. The polymer backbone encapsulates the peptide molecules and contracts to form insoluble primary complex particles. In step (d), hydrogenated phospholipids are introduced. Phospholipid molecules spontaneously arrange themselves with their hydrophobic ends facing inward and their hydrophilic ends facing outward, assembling into a stable shell structure around the particles. In step (e), the irregular clumps are depolymerized using high-frequency fluid shear force, cavitation effect, and collision action generated by a high-pressure homogenizer, refining the particles and distributing them in the range of 260 nm to 780 nm. This particle size range ensures sufficient specific surface area to improve adhesion to the skin surface, while also ensuring the thermodynamic stability of the microcapsules in the suspension, preventing sedimentation and stratification.

[0015] This invention provides a shower gel based on the synergistic repair of complex functional peptides and its preparation method. It has the following beneficial effects: 1. This invention effectively solves the problem of water-soluble active peptide substances being easily lost in large quantities with water flow in rinse-off bath products by introducing peptide-carrying composite microcapsules encapsulated by cationic polymers. The cationic polymer on the outer layer of the microcapsule carries a high density of positive charge, which can generate electrostatic adsorption with the negatively charged stratum corneum of human skin. This adsorption force is greater than the rinsing force of water flow and surfactants. After bathing, the microcapsules can stably adhere to the skin surface and continuously release the peptide components inside, thus prolonging the effective action time of the active substances on the skin.

[0016] 2. This invention uses three peptides with different functions in a specific ratio to achieve multi-target repair of physiological symptoms caused by staying up late. The first peptide in the system can competitively block the release of neurotransmitters, reduce the frequency of muscle contraction, and relieve local muscle stiffness and soreness; the second peptide can promote the synthesis of endogenous collagen in the skin and accelerate the reconstruction of damaged skin barrier; the third peptide can remove reactive oxygen free radicals accumulated in skin tissue through antioxidant mechanism, improve dull skin tone. The three peptide components work synergistically to improve the common physical fatigue and rough skin problems of people who stay up late.

[0017] 3. This invention combines aromatic components with the above-mentioned repair system and adopts a segmented temperature control process during preparation, taking into account both the product's efficacy stability and auxiliary calming effect. In the preparation method, peptide-loaded composite microcapsules and water-soluble active substances are added at a low temperature stage below 40°C to avoid peptide inactivation and microcapsule structure deformation caused by high temperature. At the same time, the volatile small molecules in aromatic components such as lavender essential oil and vetiver essential oil act on the central nervous system through the human olfactory nerve pathway, which can reduce the excitability of the sympathetic nerves during bathing and help shorten the sleep latency after staying up late. Attached Figure Description

[0018] Figure 1 This is a flowchart of the preparation method of the present invention. Detailed Implementation

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] I. Raw materials: The main raw materials and reagents used in the following examples and comparative examples have the following sources and specifications. Reagents not specifically mentioned are all commercially available analytical grade or higher grade products.

[0021] Acetyl hexapeptide-8 is a synthetic small peptide composed of six amino acids. It has the function of inhibiting the release of neurotransmitters and is used to relieve muscle tension and nervous fatigue caused by staying up late.

[0022] Dipeptide diaminobutyryl benzylamide diacetate, also known as snake venom-like peptide, is a synthetic tripeptide that mimics the active site of snake venom toxins. It achieves deep muscle relaxation by blocking acetylcholine receptors at the neuromuscular junction.

[0023] Palmitoyl tripeptide-5 is a synthetic tripeptide linked with a palmitoyl chain. Its repeating unit structure is well-defined and can activate skin transforming growth factor, promote collagen synthesis, and enhance the skin barrier.

[0024] Copper tripeptide-, chemically known as glycyl-L-histyl-L-lysine copper complex, is a functional tripeptide chelate used to promote wound healing and biorepair of barrier tissues.

[0025] Glutathione is a substance containing A tripeptide with an amide bond and a thiol group, composed of glutamic acid, cysteine ​​and glycine, it mainly functions as an antioxidant and a scavenger of reactive oxygen free radicals.

[0026] Carnosine, chemical name -Alanyl-L-histidine, as a natural dipeptide, protects skin proteins from oxidative damage through its anti-glycation effect in formulations.

[0027] Polyquaternium-10 is a cationic hydroxyethyl cellulose ether derivative whose repeating unit is 2-(2-(2-hydroxy-3-(trimethylammonium)propoxy)ethoxy)ethyl cellulose ether chloride, with a weight-average molecular weight of for It utilizes its high positive charge density to self-assemble with negatively charged peptides to form stable composite microcapsules.

[0028] Polyquaternium-7, chemically known as a copolymer of dimethyl diallyl ammonium chloride and acrylamide, has a weight-average molecular weight of [missing information]. for It is used in the system to construct a cationic shell with strong charge neutralization ability, thereby improving the adhesion rate of peptide components to the skin.

[0029] Hydroxypropyl methylcellulose is a nonionic cellulose mixed ether with repeating units of dehydrated glucose units containing hydroxypropyl and methyl substituents. Its weight-average molecular weight is... for It is mainly used as a film-forming agent and physical stabilizer for microcapsule systems.

[0030] Hydrogenated phospholipids are lipid mixtures with hydrogenated phosphatidylcholine as the main component, with a purity greater than 90%. They enhance the permeability of microcapsule systems and their affinity for biological membranes by forming lipid membrane structures.

[0031] Lavender essential oil is a complex volatile mixture extracted from the flowers of narrow-leaved lavender. Its main active ingredients are linalyl acetate and linalool, which are used to help relax the nervous system and regulate sleep quality.

[0032] Vetiver essential oil is an essential oil obtained from the roots of vetiver through distillation. It is rich in vetiverol and vetiverone, and has excellent settling and deep calming effects.

[0033] Valerian extract is a natural active product extracted from the roots of valerian. It contains valerenoic acid and sesquiterpenoids, and is used to shorten the time it takes to fall asleep through a biofeedback mechanism.

[0034] Ceramide NP, chemically known as ceramide 3, is a ceramide-like substance composed of plant sphingosine and saturated fatty acids. In the formula, it plays a role in repairing the underlying barrier and locking in moisture.

[0035] Sodium hyaluronate is a linear polymer composed of D-glucuronic acid and N-acetyl-D-glucosamine disaccharide units, with a weight-average molecular weight of for It has significant surface film-forming and moisture-absorbing properties.

[0036] The other raw materials used in this invention, such as cocoyl glutamic acid TEA salt, cocamidopropyl betaine, bergamot essential oil, and conventional regulators, are all commercially available products in the daily chemical industry.

[0037] II. Preparation Example: Preparation Example 1: This preparation example provides a cationic-peptide complex microcapsule for use in an anti-late-night shower gel, comprising the following steps: (1) Aqueous phase preparation: Dissolve 0.3 kg acetyl hexapeptide-8, 0.3 kg palmitoyl tripeptide-5 and 0.4 kg glutathione in 20 kg deionized water at a weight ratio of 3:3:4 and stir at 200 RPM for 20 minutes at 25°C to obtain a polypeptide premix. (2) Charge adjustment: Under stirring, the pH of the polypeptide premix was precisely adjusted to 4.5 using a 5% citric acid solution to fully expose the anionic charge sites of the polypeptide molecules; (3) Self-assembly into nuclei: 5 kg of 10% polyquaternium-7 solution was slowly added to the polypeptide premix. The high charge density cationic polymer and the negatively charged polypeptide molecules were electrostatically self-assembled to form primary composite micronuclei. (4) Microcapsule stabilization: Add 0.1 kg of hydrogenated phospholipids to the system and continue stirring for 15 minutes. A biomimetic protective layer is formed on the surface of the microcapsules through the hydrophobic interaction of lipid molecules. (5) Homogenization: The above mixture was placed in a high-pressure homogenizer and circulated twice under a pressure of 20 MPa to obtain a peptide-loaded composite microcapsule suspension with an average particle size of 780 nm.

[0038] Preparation Example 2: This preparation example provides a cationic-peptide complex microcapsule for use in an anti-late-night shower gel, comprising the following steps: (1) Aqueous phase preparation: 0.3 kg dipeptide diaminobutyryl benzylamide diacetate, 0.3 kg copper tripeptide-1 and 0.4 kg carnosine were fully dissolved in 25 kg deionized water at a weight ratio of 3:3:4. The mixture was stirred at 300 RPM for 25 minutes at 30°C to obtain a polypeptide premix. (2) Charge adjustment: The pH of the peptide premix was precisely adjusted to 5.0 using a 5% citric acid solution to maintain the system in a stable charge balance range; (3) Self-assembly into nucleus: 6 kg of 12% polyquaternium-10 solution was slowly added dropwise to the polypeptide premix to induce the polypeptide molecules to bind with the cationic cellulose backbone to form a stable charge complex; (4) Microcapsule stabilization: 0.3 kg of hydrogenated phospholipid and 0.5 kg of hydroxypropyl methylcellulose were added to the system in sequence, and the mixture was stirred for 20 minutes to enhance the anti-aggregation stability of the microcapsules by utilizing the steric hindrance effect of the polymer. (5) Homogenization: The mixture was placed in a high-pressure homogenizer and circulated 3 times under a pressure of 30 MPa to obtain a peptide-loaded composite microcapsule suspension with an average particle size of 520 nm.

[0039] Preparation Example 3: This preparation example provides a cationic-peptide complex microcapsule for an anti-late-night shower gel, comprising the following steps: (1) Aqueous phase preparation: Dissolve 0.3 kg acetyl hexapeptide-8, 0.3 kg copper tripeptide-1 and 0.4 kg glutathione in 30 kg deionized water at a weight ratio of 3:3:4 and stir at 400 RPM for 30 minutes at 35°C to obtain a polypeptide premix. (2) Charge adjustment: The pH of the peptide premix was precisely adjusted to 5.5 using a 5% citric acid solution to ensure that all peptide components were in a fully ionized state; (3) Self-assembly and nucleation: Under rapid stirring, 8 kg of a 15% polyquaternium-10 solution was added dropwise to the peptide premix to achieve complete encapsulation and immobilization of the peptide molecules through high positive charge density; (4) Microcapsule stabilization: 0.5 kg of hydrogenated phospholipid and 1.0 kg of hydroxypropyl methylcellulose were added to the system in sequence to construct a dense hydrophilic composite membrane layer on the outer layer of the microcapsules; (5) Homogenization: The mixture was placed in a high-pressure homogenizer and circulated 3 times under a pressure of 40 MPa to obtain a peptide-loaded composite microcapsule suspension with an average particle size of 260 nm.

[0040] III. Examples Example 1: This example provides an anti-late-night body care shower gel based on the synergistic repair of compound functional peptides. The composition ratio, by weight percentage (w / w), is as follows: 0.5% of the peptide-loaded compound microcapsules provided in Preparation Example 1, 12.0% of cocoyl glutamate TEA salt, 3.0% of cocamidopropyl betaine, 0.2% of lavender essential oil, 0.2% of bergamot essential oil, 0.1% of vetiver essential oil, 0.05% of ceramide NP, 0.1% of sodium hyaluronate, 0.05% of bisabolol, 0.1% of panthenol, appropriate amount of citric acid, and balance of deionized water; Includes the following steps: (1) Matrix pretreatment: Heat deionized water to 70°C, add ceramide NP, cocoyl glutamic acid TEA salt and cocamidopropyl betaine, and stir at 100 RPM until the solid is completely dissolved to form a uniform matrix solution; (2) Cooling stage: Start the cooling cycle to reduce the temperature of the base liquid to 35℃; (3) Addition of core components: Slowly add the peptide-loaded composite microcapsules, sodium hyaluronate, bisabolol and panthenol provided in Preparation Example 1 to the base liquid, and keep stirring at a low speed of 50 RPM for 15 minutes to ensure uniform dispersion; (4) Aroma system introduction: After premixing lavender essential oil, bergamot essential oil, vetiver essential oil and 1.0% polysorbate-20 evenly, slowly add it to the main tank and stir for 10 minutes; (5) Finished product adjustment: Use citric acid to adjust the pH of the system to 5.5, stir evenly and let stand to remove bubbles to obtain the finished product.

[0041] Example 2: This example provides an anti-late-night body care shower gel based on the synergistic repair of compound functional peptides. The composition ratio, by weight percentage (w / w), is as follows: 1.5% of the peptide-loaded compound microcapsules provided in Preparation Example 2, 15.0% of cocoyl glutamate TEA salt, 5.0% of cocamidopropyl betaine, 0.4% of lavender essential oil, 0.4% of bergamot essential oil, 0.2% of vetiver essential oil, 0.1% of ceramide NP, 0.3% of sodium hyaluronate, 0.1% of bisabolol, 0.2% of panthenol, appropriate amount of citric acid, and the balance of deionized water; Includes the following steps: (1) Matrix pretreatment: Heat deionized water to 70°C, add ceramide NP and surfactant system, and stir at 150 RPM until the system is clear and transparent; (2) Cooling stage: Reduce the temperature of the base liquid to 38℃; (3) Addition of core components: Add the peptide-loaded composite microcapsules, sodium hyaluronate, bisabolol and panthenol provided in Preparation Example 2 in sequence, and stir at 80 RPM for 20 minutes; (4) Aroma system introduction: Premix the essential oil components with 2.0% PEG-40 hydrogenated castor oil, add them to the main tank, and control the stirring speed at 100 RPM; (5) Finished product adjustment: Use citric acid to adjust the pH of the system to 5.8, measure the viscosity and fill.

[0042] Example 3: This example provides an anti-late-night body care shower gel based on the synergistic repair of compound functional peptides. The composition ratio, by weight percentage (w / w), is as follows: 3.0% of the peptide-loaded compound microcapsules provided in Preparation Example 3, 20.0% of cocoyl glutamate TEA salt, 8.0% of cocamidopropyl betaine, 0.8% of lavender essential oil, 0.8% of bergamot essential oil, 0.4% of vetiver essential oil, 0.2% of ceramide NP, 0.5% of sodium hyaluronate, 0.2% of bisabolol, 0.4% of panthenol, appropriate amount of citric acid, and balance of deionized water; Includes the following steps: (1) Matrix pretreatment: Heat deionized water to 75°C, add surfactant and ceramide NP, and stir at 200 RPM until completely mixed; (2) Cooling stage: Rapidly cool down to 40℃; (3) Addition of core components: Add the peptide-loaded composite microcapsules, sodium hyaluronate and repairing agent provided in Preparation Example 3, and stir at 100 RPM for 30 minutes to ensure that the high concentration of active components is fully and evenly distributed; (4) Aroma system introduction: Add the mixture of essential oil and solubilizer, and continue stirring until the system becomes clear again; (5) Finished product adjustment: Use citric acid to adjust the pH of the system to 6.0 to obtain the finished product.

[0043] Example 4: This example provides an anti-late-night body care shower gel based on the synergistic repair of compound functional peptides. The composition ratio, by weight percentage (w / w), is as follows: 2.0% of the peptide-loaded compound microcapsules provided in Preparation Example 2, 18.0% of cocoyl glutamate TEA salt, 6.0% of cocamidopropyl betaine, 1.0% of valerian extract, 0.5% of lavender essential oil, 0.15% of ceramide NP, 0.4% of sodium hyaluronate, appropriate amount of citric acid, and the balance of deionized water; Includes the following steps: (1) Matrix pretreatment: Heat deionized water to 70°C to dissolve surfactant and ceramide NP; (2) Cooling stage: Cool down to 37℃; (3) Addition of core components: Add the peptide-loaded composite microcapsules, sodium hyaluronate and valerian extract provided in Preparation Example 2, and stir at low speed until uniform; (4) Aroma system introduction: Add lavender essential oil premix; (5) Finished product adjustment: Adjust the pH value to 5.7 and let it stand to remove bubbles.

[0044] Example 5: This example provides an anti-late-night body care shower gel based on the synergistic repair of compound functional peptides. The component ratio, by weight percentage (w / w), is as follows: 1.0% of the peptide-loaded compound microcapsules provided in Preparation Example 1, 14.0% of cocoyl glutamate TEA salt, 4.0% of cocamidopropyl betaine, 0.6% of bergamot essential oil, 0.4% of vetiver essential oil, 0.1% of ceramide NP, 0.1% of bisabolol, 0.3% of panthenol, appropriate amount of citric acid, and the balance of deionized water; including the following steps: (1) Matrix pretreatment: Heat deionized water to 70°C to dissolve surfactant and ceramide NP; (2) Cooling stage: Cool down to 36℃; (3) Addition of core components: Add the peptide-loaded composite microcapsules, bisabolol and panthenol provided in Preparation Example 1; (4) Aroma system introduction: Add essential oil components and stir well; (5) Finished product adjustment: Adjust the pH value to 5.9 to obtain the finished product.

[0045] IV. Comparative Examples Comparative Example 1: Compared with Example 2, the difference is that a free polypeptide composition with the same content as the peptide-loaded composite microcapsules in Example 2 was directly added (i.e., the cationic self-assembly coating process in Preparation Example 2 was not performed), while the other components and preparation steps were the same.

[0046] Comparative Example 2: Compared with Example 2, the difference is that the core polypeptide component was replaced with an equal weight of single acetyl hexapeptide-8, and it does not contain barrier repair peptides and antioxidant skin brightening peptides. The remaining components and preparation steps are the same.

[0047] Comparative Example 3: Compared with Example 2, the difference is that the surfactant system (cocoyl glutamate TEA salt and cocamidopropyl betaine) was replaced with an equal weight of sodium lauryl ether sulfate (SLES), while the other components and preparation steps were the same.

[0048] Comparative Example 4: Compared with Example 2, the difference is that the formula does not contain the sleep-aiding essential oil system (lavender essential oil, bergamot essential oil, vetiver essential oil), and the missing proportion is made up with deionized water. The other components and preparation steps are the same.

[0049] Comparative Example 5: Compared with Example 2, the difference is that in the preparation of peptide-loaded microcapsules, the cationic polymer (polyquaternium-10) was replaced with an equal weight of the anionic polymer (sodium carboxymethyl cellulose), while the other components and preparation steps were the same.

[0050] Comparative Example 6: Compared with Example 2, the difference is that in step (3) of the shower gel preparation process, no cooling treatment is performed, and the peptide-loaded composite microcapsules and active additives are added directly at a high temperature of 70°C. All other components are the same.

[0051] Comparative Example 7: Compared with Example 2, the difference is that the ratio of the core polypeptide was changed, and the weight ratio of acetyl hexapeptide-8, copper tripeptide-1 and carnosine was adjusted from 3:3:4 to 1:1:1, while the total content remained unchanged. The remaining components and preparation steps were the same.

[0052] V. Test Example: The following are adjustments and optimizations to the experimental steps and conclusions section of Test Example 1.

[0053] 1. Experimental Procedure Twelve subjects aged 22 to 45 years were selected. They discontinued using body care products 24 hours prior to the trial. Three 5cm x 5cm areas were marked on the inner forearm of each subject. 2.0g of samples from Example 2, Comparative Example 1, and Comparative Example 5 were applied to each area, and the mixture was massaged for 1 minute. Each area was then rinsed with deionized water at 35°C and a flow rate of 2.0L / min for 30 seconds. After blotting with filter paper and drying at room temperature for 5 minutes, extraction was performed using a cup extraction method. A 3cm inner diameter extraction cup was pressed firmly onto the skin of the test area, and 3.0mL of the sample was added. A 1:1 pH 7.4 phosphate buffer solution was mixed with acetonitrile and shaken for 5 minutes to elute peptide components retained on the skin surface. The extract was collected and the extraction was repeated once. The two liquids were combined and filtered through a 0.22 μm filter membrane. High-performance liquid chromatography (HPLC) was used for detection. The chromatographic conditions were a C18 reversed-phase column, an acetonitrile-water solution containing 0.1% trifluoroacetic acid as the mobile phase, a detection wavelength of 220 nm, and a flow rate of 1.0 mL / min. The concentration of acetyl hexapeptide-8 in the sample was calculated based on the standard curve and converted into the amount retained per unit area.

[0054] 2. Experimental Data The table below records the post-wash residue levels of acetyl hexapeptide-8 on the skin surface of 12 subjects under different treatment conditions.

[0055] Table 1: Results of Acetylhexapeptide-8 Retention Amount on Skin Surface After Washing (μg / cm³) 2 )

[0056] 3. Test Conclusion Experimental data showed that the retention amount of acetyl hexapeptide-8 after washing varied under different treatment conditions. The isoelectric point of human skin is approximately 4.2 to 5.0. Under bathing conditions, keratin carries a negative charge. The free polypeptide in Comparative Example 1 is distributed in a molecular state, with weak interaction with the skin surface, and is easily washed away with surfactants and water flow. The average retention amount was measured to be 1.13 μg / cm³. 2 Comparative Example 5 used anionic polymers to construct microcapsules, whose negative surface charge generated electrostatic repulsion with the skin surface, reducing the average retention to 0.43 μg / cm³. 2 Example 2 uses polyquaternium-10 and peptides to assemble cationic microcapsules. The positive charge on the surface of the microcapsules generates electrostatic adsorption with the stratum corneum of the skin, overcoming the shear force of water rinsing, with an average retention of 12.33 μg / cm³. 2 This result demonstrates that cationic microcapsule structures can achieve physical retention of peptide components on the skin surface.

[0057] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A shower gel based on the synergistic repair of complex functional peptides, characterized in that, The shower gel contains the following components by weight percentage: peptide complex microcapsules 0.5%–3.0%; cocoyl glutamic acid TEA salt 12.0%–20.0%; cocamidopropyl betaine 3.0%–8.0%; fragrance components 0.5%–2.0%; repairing aids 0.2%–1.5%; solubilizer 0–2.0%; citric acid, the amount added to make the pH of the shower gel 5.5–6.0; and deionized water as the balance.

2. The shower gel based on the synergistic repair of compound functional peptides according to claim 1, characterized in that, The peptide-loaded composite microcapsules are made from the following raw materials in parts by weight: 1 part polypeptide composition; 0.5 to 1.2 parts cationic polymer; 0.1 to 0.5 parts hydrogenated phospholipid; and 0 to 1.0 parts hydroxypropyl methylcellulose.

3. The shower gel based on the synergistic repair of compound functional peptides according to claim 2, characterized in that, The polypeptide composition comprises a first peptide, a second peptide, and a third peptide, wherein the weight ratio of the first peptide, the second peptide, and the third peptide is 3:3:4; the first peptide is selected from one or a combination of two of acetyl hexapeptide-8 and dipeptide diaminobutyryl benzylamide diacetate; the second peptide is selected from one or a combination of two of palmitoyl tripeptide-5 and copper tripeptide-1; and the third peptide is selected from one or a combination of two of glutathione and carnosine.

4. The shower gel based on the synergistic repair of compound functional peptides according to claim 2, characterized in that, The cationic polymer is selected from one or a combination of two of polyquaternium-7 and polyquaternium-10.

5. A shower gel based on the synergistic repair of compound functional peptides according to claim 1, characterized in that, The aromatic component is selected from one or more of lavender essential oil, bergamot essential oil, vetiver essential oil, and valerian extract; the solubilizer is selected from one or more of polysorbate-20 and PEG-40 hydrogenated castor oil.

6. The shower gel based on the synergistic repair of compound functional peptides according to claim 1, characterized in that, The repair aids include ceramide NP, sodium hyaluronate, bisabolol, and panthenol.

7. A method for preparing a shower gel based on the synergistic repair of complex functional peptides according to any one of claims 1-6, characterized in that, Includes the following steps: Matrix pretreatment: Heat deionized water to 70℃~75℃, add cocoyl glutamic acid TEA salt, cocamidopropyl betaine and ceramide NP from the repair adjuvant, and stir at 100~200RPM until the solids are completely dissolved to form a uniform base solution. Cooling stage: The temperature of the homogeneous base liquid is reduced to 35℃~40℃; Core component addition: Add peptide-loaded composite microcapsules to the homogeneous base liquid, and add the remaining components of the repair aid except for ceramide NP. Stir at 50-100 RPM for 15-30 minutes to ensure uniform dispersion. Aromatic system introduction: After the aromatic components and solubilizer are premixed evenly to form a mixture, the mixture is added to the main tank and stirred evenly; Finished product adjustment: Adjust the pH of the system to 5.5-6.0 using citric acid, let it stand to remove bubbles, and obtain the finished product.

8. The method for preparing a shower gel based on the synergistic repair of complex functional peptides according to claim 1, characterized in that, Before adding the core components, the peptide-loaded composite microcapsules are pre-prepared as follows: Aqueous phase preparation: Dissolve the polypeptide composition in deionized water to obtain a polypeptide premix; Charge adjustment: While stirring, the pH of the polypeptide premix was adjusted to 4.5–5.5 using a 5% (w / w) citric acid solution; Self-assembly into nuclei: A cationic polymer solution is added dropwise to the premixed polypeptide solution after pH adjustment to perform electrostatic self-assembly and form a primary composite micronucleus; Microcapsule stabilization: Hydrogenated phospholipids and hydroxypropyl methylcellulose are added to the system containing the primary composite micronucleus, and stirring is continued to form a mixture containing microcapsules; Homogenization: The mixture containing microcapsules is placed in a high-pressure homogenizer and circulated 2 to 3 times under a pressure of 20 to 40 MPa to obtain a peptide-loaded composite microcapsule suspension.

9. The preparation method of a shower gel based on the synergistic repair of complex functional peptides according to claim 8, characterized in that, The average particle size of the microcapsules in the peptide-loaded composite microcapsule suspension obtained in step (e) is 260 nm to 780 nm.

10. The method for preparing a shower gel based on the synergistic repair of complex functional peptides according to claim 7, characterized in that, In the process of adding core components, the remaining components of the repair aid, excluding ceramide NP, are sodium hyaluronate, bisabolol, and panthenol.

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