A pretreatment device for detecting mouse tumor tissue and normal tissue by LIBS

Abstract

The application relates to the technical field of tissue processing, in particular to a pretreatment device for LIBS (Laser-Induced Breakdown Spectroscopy) detection of mouse tumor tissue and normal tissue, which comprises a pretreatment frame; a spraying device is fixedly connected to one side of the pretreatment frame; a lifting device is installed above the spraying device; a one-piece frame in sliding connection with the pretreatment frame is fixedly connected to the free end of the lifting device; symmetrical spraying mechanisms are installed in the one-piece frame; hoses are communicated with the output ends of the spraying device; the other ends of the hoses are communicated with the spraying mechanisms; and a gas purification all-in-one machine for purifying external air is further installed in the pretreatment frame. The device can realize integrated processing of fixation, dehydration and drying of the tissue, improve the processing efficiency of the tissue, ensure sample consistency, and guarantee the reliability of subsequent detection data results.

Description

Technical Field

[0001] This invention relates to the field of tissue processing technology, specifically a pretreatment device for LIBS detection of mouse tumor tissue and normal tissue. Background Technology

[0002] Rapid differentiation between tumor and normal tissues is crucial for tumor research. Current tissue identification methods largely rely on pathological staining and mass spectrometry, which suffer from drawbacks such as cumbersome pretreatment and lengthy detection times. Laser-induced breakdown spectroscopy (LIBS) enables rapid in-situ elemental analysis of samples and holds promise for applications in biological tissue identification. However, current LIBS research on mouse tumor tissue detection faces challenges such as the susceptibility of soft tissues to deformation, interference from moisture and fixatives with laser ablation effects, a lack of standardized sample preparation procedures, and difficulties in consistently obtaining effective spectral signals.

[0003] The existing mouse tissue sample preparation process adapted for LIBS detection is as follows: Suitable-age experimental mice are selected, and normal tissue and breast tumor tissue are separated. These are then sequentially immersed in a neutral, universal tissue fixative for 0.5 hours for rapid fixation. The tissues are then rinsed twice with anhydrous ethanol to remove residual fixative and achieve preliminary dehydration. Finally, the tissue blocks are air-dried in a clean environment to obtain samples that meet the requirements for laser ablation. This entire process requires manual step-by-step execution, is cumbersome, and has low automation. Inconsistent operating techniques and processing times among different operators can easily lead to significant differences in sample dehydration and surface condition, compromising sample processing consistency and reducing the repeatability of subsequent LIBS spectral acquisition, thus affecting the reliability of tumor tissue identification and detection results. Therefore, to address these issues, a pretreatment device for LIBS detection of mouse tumor and normal tissues is proposed. Summary of the Invention

[0004] The purpose of this invention is to provide a pretreatment device for LIBS detection of mouse tumor tissue and normal tissue, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: As an optional embodiment of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue includes a pretreatment frame, an operation panel and a tissue cleaning mechanism. A spraying device is fixedly connected to one side of the pretreatment frame. A lifting device is installed above the spraying device. An integral frame that is slidably connected to the free end of the lifting device is fixedly connected to the pretreatment frame. Spraying mechanisms are installed symmetrically inside the integral frame. The output end of the spraying equipment is connected to a hose, and the other end of the hose is connected to the spraying mechanism. An operation panel is also installed on one side of the pretreatment frame. A tissue cleaning mechanism connected to the pretreatment frame is set on one side of the operation panel. A gas purification unit for purifying external air is also installed inside the pretreatment frame. The output end of the gas purification unit is connected to the tissue cleaning mechanism.

[0006] The existing mouse tissue sample preparation process adapted for LIBS detection is as follows: Suitable-age experimental mice are selected, and normal tissue and breast tumor tissue are separated. These are then sequentially immersed in a neutral, universal tissue fixative for 0.5 hours for rapid fixation. The tissues are then rinsed twice with anhydrous ethanol to remove residual fixative and achieve preliminary dehydration. Finally, the tissue blocks are air-dried in a clean environment to obtain test samples that meet the requirements for laser ablation. The entire process requires manual step-by-step completion, is cumbersome, and has a low degree of automation. The inconsistent operating techniques and processing times of different operators can easily lead to significant differences in the degree of sample dehydration and surface condition, compromising sample processing consistency and reducing the repeatability of subsequent LIBS spectral acquisition. This affects the reliability of tumor tissue identification and detection results. By placing the tissue inside an integrated frame, which is then lowered into the pretreatment frame via a lifting device, tissue fixation can be achieved. After fixation, the lifting device moves the integrated frame upward, separating the tissue from the fixative. The tissue is then rapidly cleaned and pre-dehydrated by activating the spray equipment and mechanism. Finally, the tissue is placed in a tissue cleaning unit for drying. The fixation, dehydration, and drying processes can be standardized, improving tissue processing efficiency, ensuring sample consistency, and guaranteeing the reliability of subsequent detection data.

[0007] As an optional solution of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, the tissue cleaning mechanism includes a cleaning frame, a cover plate and a drain plate. The drain plate is installed inside the cleaning frame, and a uniformly distributed placement plate is fixedly connected above the drain plate. The cover plate is provided above the placement plate. The clean frame has a ventilation frame connected to its side, and one end of the ventilation frame is connected to the output end of the integrated gas purification unit.

[0008] As an optional embodiment of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, the placement plates are arranged in multiple groups and are inclined.

[0009] As an optional embodiment of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, the surfaces of the drain plate and the placement plate are provided with uniformly distributed ventilation holes.

[0010] As an optional embodiment of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, the bottom of the clean frame is connected to a collection tube, and the other end of the collection tube is located on the outside of the pretreatment frame.

[0011] As an optional embodiment of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, a breathable hood is embedded at the end of the clean frame away from the ventilation frame.

[0012] After cleaning, the tissue is placed on the surface of an inclined placement plate. Activating the integrated gas purification machine allows clean air to pass through the placement plate. This serves two purposes: firstly, it enables simultaneous drying of the inclined tissue on both sides, improving drying efficiency; secondly, the inclined placement plate can accommodate a large sample area, ensuring sample processing efficiency. Furthermore, the unidirectional airflow ensures that external air does not easily contaminate the sample, guaranteeing accurate test data.

[0013] As an optional solution of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, the spraying mechanism includes a mounting plate fixedly connected to an integral frame, a mounting frame installed on one side of the bottom of the mounting plate, a telescopic device fixedly connected inside the mounting frame, an adjustment frame fixedly connected to the other end of the telescopic device, and an evenly distributed adjustment nozzle inside the adjustment frame. The top of the adjustment nozzle is connected to the mounting plate and is rotatably connected to it. One side of the mounting plate is connected to the flexible hose.

[0014] As an optional embodiment of the pretreatment device for LIBS detection of mouse tumor tissue and normal tissue described in this invention, the adjustment frame has uniformly distributed grooves inside, and adjustment columns fixedly connected to the adjustment frame are provided in the grooves, with the other end of each adjustment column slidably connected to the outside of the adjustment nozzle.

[0015] During tissue cleaning, the telescopic device moves the adjustment frame back and forth, which in turn moves the adjustment column back and forth, allowing the adjustment nozzle to rotate. This enables large-area spray cleaning of the tissue inside the integrated frame, and the cleaning quality can be ensured by manually turning the tissue over.

[0016] Compared with the prior art, the beneficial effects of the present invention are: The tissue is placed inside an integrated frame, which is then lowered into the pretreatment frame via a lifting device. This allows for tissue fixation. After fixation, the integrated frame is raised by the lifting device, separating the tissue from the fixative. The tissue is then quickly cleaned by activating the spray equipment and mechanism, achieving initial dehydration. Finally, the tissue is placed in the tissue cleaning unit for drying. The fixation, dehydration, and drying processes can be standardized, improving tissue processing efficiency, ensuring sample consistency, and guaranteeing the reliability of subsequent test data. During the cleaning of the tissue, the telescopic device moves the adjustment frame back and forth, which in turn moves the adjustment column back and forth, allowing the adjustment nozzle to rotate. This allows for large-area spray cleaning of the tissue inside the integrated frame, and the cleaning quality can be ensured by manually turning the tissue over. After cleaning, the tissue is placed on the surface of an inclined placement plate. Activating the integrated gas purification machine allows clean air to pass through the placement plate. This serves two purposes: firstly, it enables simultaneous drying of the inclined tissue on both sides, improving drying efficiency; secondly, the inclined placement plate can accommodate a large sample area, ensuring sample processing efficiency. Furthermore, the unidirectional airflow ensures that external air does not easily contaminate the sample, guaranteeing accurate test data. Attached Figure Description

[0017] Figure 1 A schematic diagram of the overall structure of a pretreatment device for LIBS detection of mouse tumor tissue and normal tissue; Figure 2 This is a schematic diagram of the tissue cleaning mechanism of a pretreatment device for LIBS detection of mouse tumor tissue and normal tissue. Figure 3 This is a schematic diagram of the spraying mechanism of a pretreatment device for LIBS detection of mouse tumor tissue and normal tissue. Figure 4 This is a schematic diagram of the installation structure of the adjustment column in a pretreatment device for LIBS detection of mouse tumor tissue and normal tissue.

[0018] In the diagram: 1-Pretreatment frame, 2-Operation panel, 3-Organizational cleanroom mechanism, 301-Cleanroom frame, 302-Cover plate, 303-Draining plate, 304-Placement plate, 305-Ventilation frame, 306-Air hood, 307-Collection pipe, 4-Spraying equipment, 5-Hose, 6-Lifting equipment, 7-Integrated frame, 8-Spraying mechanism, 801-Mounting plate, 802-Mounting frame, 803-Telescopic equipment, 804-Adjusting frame, 805-Adjusting column, 806-Adjusting nozzle, 9-Integrated gas purification machine. Detailed Implementation

[0019] Example 1: Please refer to Figure 1The present invention provides a technical solution: A pretreatment device for LIBS detection of mouse tumor tissue and normal tissue includes a pretreatment frame 1, an operation panel 2, and a tissue cleaning mechanism 3. A spraying device 4 is fixedly connected to one side of the pretreatment frame 1. A lifting device 6 is installed above the spraying device 4. An integral frame 7 that is slidably connected to the free end of the lifting device 6 is fixedly connected to the pretreatment frame 1. Spraying mechanisms 8 are installed symmetrically inside the integral frame 7. The output end of the spraying device 4 is connected to a hose 5, and the other end of the hose 5 is connected to the spraying mechanism 8. An operation panel 2 is also installed on one side of the pretreatment frame 1. A tissue cleaning mechanism 3 connected to the pretreatment frame 1 is provided on one side of the operation panel 2. A gas purification unit 9 for purifying external air is also installed inside the pretreatment frame 1. The output end of the gas purification unit 9 is connected to the tissue cleaning mechanism 3.

[0020] The existing mouse tissue sample preparation process adapted for LIBS detection is as follows: Suitable-age experimental mice are selected, and normal tissue and breast tumor tissue are separated. These are then sequentially immersed in a neutral, universal tissue fixative for 0.5 hours for rapid fixation. The tissues are then rinsed twice with anhydrous ethanol to remove residual fixative and achieve preliminary dehydration. Finally, the tissue blocks are air-dried in a clean environment to obtain test samples that meet the requirements for laser ablation. The entire process requires manual step-by-step completion, is cumbersome, and has a low degree of automation. The inconsistent operating techniques and processing times of different operators can easily lead to significant differences in the degree of sample dehydration and surface condition, making it impossible to guarantee sample processing consistency. This reduces the repeatability of subsequent LIBS spectral acquisition and affects the reliability of tumor tissue identification and detection results. By placing the tissue inside the integrated frame 7 and lowering it into the pretreatment frame 1 via the lifting device 6, tissue fixation can be achieved. After fixation, the lifting device 6 moves the integrated frame 7 upward, separating the tissue from the fixative. The spray device 4 and spray mechanism 8 are then activated to quickly clean the tissue and complete the initial dehydration. The tissue is then placed in the tissue cleaning mechanism 3 for drying. The fixation, dehydration, and drying of the tissue can be standardized, which can improve the efficiency of tissue processing, ensure sample consistency, and guarantee the reliability of subsequent detection data results.

[0021] Also includes the following: During the cleaning of the tissue, the hose 5 ensures that the integrated frame 7 moves upward smoothly, so that its bottom is separated from the fixing liquid below. Then, the spraying mechanism 8 enables the simultaneous cleaning of multiple tissues, and the surface of multiple tissues is thoroughly cleaned by manual turning.

[0022] Example 2: This example is an improvement upon Example 1. Please refer to [link / reference]. Figure 2 and Figure 3 Specifically, the clean organization 3 includes a clean frame 301, a cover plate 302 and a drain plate 303. The drain plate 303 is installed inside the clean frame 301. A uniformly distributed placement plate 304 is fixedly connected above the drain plate 303. The cover plate 302 is provided above the placement plate 304. The side of the clean frame 301 is connected to the ventilation frame 305, and one end of the ventilation frame 305 is connected to the output end of the gas purification integrated machine 9.

[0023] The placement plates 304 are arranged in multiple groups and are inclined.

[0024] Both the drain plate 303 and the placement plate 304 have evenly distributed ventilation holes on their surfaces.

[0025] The bottom of the clean frame 301 is connected to a collection pipe 307, and the other end of the collection pipe 307 is located on the outside of the pretreatment frame 1.

[0026] A breathable cover 306 is embedded at the end of the clean frame 301 away from the ventilation frame 305.

[0027] After cleaning, the tissue is placed on the surface of the inclined placement plate 304. The gas purification unit 9 is activated to drive clean air through the placement plate 304. This serves two purposes: firstly, it allows for simultaneous drying of the inclined tissue on both sides, improving drying efficiency; secondly, the inclined placement plate 304 can support a large sample area, ensuring sample processing efficiency. Furthermore, the unidirectional airflow ensures that external air does not easily contaminate the sample, guaranteeing accurate test data.

[0028] Also includes the following: The cover plate 302 facilitates sealing of the top of the clean frame 301, ensuring that external air can only be discharged through the ventilation frame 305, thus ensuring the quality of the tissue sample. When the tissue is placed on the inclined placement plate 304, water on the tissue surface can drip through the placement plate 304 and the drain plate 303 below, and be discharged through the collection pipe 307, avoiding affecting the normal drying of the tissue inside the clean frame 301. At the same time, the integrated gas purification unit 9 can treat the incoming air to ensure air quality, and the single-sided vent 306 ensures air exhaust. This configuration can ensure unidirectional airflow and prevent external air from affecting the tissue.

[0029] Example 3: This example is an improvement on Example 2. Please refer to [link / reference]. Figure 4Specifically, the spraying mechanism 8 includes a mounting plate 801 fixedly connected to the integral frame 7. A mounting bracket 802 is mounted on one side of the bottom of the mounting plate 801. A telescopic device 803 is fixedly connected inside the mounting bracket 802. An adjusting frame 804 is fixedly connected to the other end of the telescopic device 803. An adjusting nozzle 806 is evenly distributed inside the adjusting frame 804. The top of the adjusting nozzle 806 is connected to the mounting plate 801 and is rotatably connected to it. One side of the mounting plate 801 is connected to the hose 5.

[0030] The interior of the adjusting frame 804 has evenly distributed grooves, and an adjusting column 805 is fixedly connected to the adjusting frame 804 in the groove. The other end of the adjusting column 805 is slidably connected to the outside of the adjusting nozzle 806.

[0031] During the cleaning of the tissue, the telescopic device 803 drives the adjusting frame 804 to move back and forth, and the adjusting frame 804 drives the adjusting column 805 to move back and forth, thereby allowing the adjusting nozzle 806 to rotate. At this time, a large area of ​​tissue inside the integrated frame 7 can be sprayed and cleaned. Furthermore, the cleaning quality can be ensured by manually turning the tissue over.

[0032] Also includes the following: One end of the adjusting column 805 is slidably connected to the adjusting nozzle 806, which is used to drive the adjusting nozzle 806 to rotate to achieve large-area spraying. The mounting plate 801 is hollow inside, with one side connected to the hose 5 and the bottom connected to multiple sets of adjusting nozzles 806. The bottom of the integrated frame 7 is hollow, which facilitates the smooth falling of the cleaning liquid. After the cleaning work is completed, the liquid inside the pretreatment frame 1 can be uniformly treated.

[0033] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only for the purpose of helping to understand the method and core ideas of the present invention. The above descriptions are only preferred embodiments of the present invention. It should be noted that due to the limitations of textual expression, while there are objectively infinite specific structures, those skilled in the art can make several improvements, modifications, or changes without departing from the principles of the present invention, and can also combine the above technical features in an appropriate manner. These improvements, modifications, changes, or combinations, or the direct application of the inventive concept and technical solution to other situations without modification, should all be considered within the scope of protection of the present invention.

Claims

1. A pretreatment device for LIBS detection of mouse tumor tissue and normal tissue, characterized in that: It includes a pretreatment frame (1), an operation panel (2), and a tissue cleaning mechanism (3); A spraying device (4) is fixedly connected to one side of the pretreatment frame (1), and a lifting device (6) is installed above the spraying device (4). An integral frame (7) that is slidably connected to the free end of the lifting device (6) is fixedly connected to the pretreatment frame (1), and a spraying mechanism (8) that is symmetrically arranged is installed inside the integral frame (7). The output end of the spraying equipment (4) is connected to a hose (5), and the other end of the hose (5) is connected to the spraying mechanism (8); An operation panel (2) is also installed on one side of the pretreatment frame (1). A tissue cleaning mechanism (3) connected to the pretreatment frame (1) is provided on one side of the operation panel (2). A gas purification unit (9) for purifying external air is also installed inside the pretreatment frame (1). The output end of the gas purification unit (9) is connected to the tissue cleaning mechanism (3).

2. The pretreatment device for LIBS detection of mouse tumor tissue and normal tissue according to claim 1, characterized in that: The clean organization (3) includes a clean frame (301), a cover plate (302) and a drain plate (303). The clean frame (301) is equipped with a drain plate (303), and a uniformly distributed placement plate (304) is fixedly connected above the drain plate (303). The cover plate (302) is provided above the placement plate (304). The side of the clean frame (301) is connected to the ventilation frame (305), and one end of the ventilation frame (305) is connected to the output end of the gas purification unit (9).

3. The pretreatment device for LIBS detection of mouse tumor tissue and normal tissue according to claim 2, characterized in that: The placement plates (304) are arranged in multiple groups, and the placement plates (304) are arranged at an angle.

4. The pretreatment device for LIBS detection of mouse tumor tissue and normal tissue according to claim 2, characterized in that: Both the drain plate (303) and the placement plate (304) have evenly distributed ventilation holes on their surfaces.

5. The pretreatment device for LIBS detection of mouse tumor tissue and normal tissue according to claim 2, characterized in that: The bottom of the clean frame (301) is connected to a manifold (307), and the other end of the manifold (307) is located on the outside of the pretreatment frame (1).

6. The pretreatment device for LIBS detection of mouse tumor tissue and normal tissue according to claim 2, characterized in that: A breathable hood (306) is embedded at the end of the clean frame (301) away from the ventilation frame (305).

7. The pretreatment device for LIBS detection of mouse tumor tissue and normal tissue according to claim 1, characterized in that: The spraying mechanism (8) includes a mounting plate (801) fixedly connected to the integral frame (7). A mounting bracket (802) is installed on one side of the bottom of the mounting plate (801). A telescopic device (803) is fixedly connected inside the mounting bracket (802). An adjustment frame (804) is fixedly connected to the other end of the telescopic device (803). An evenly distributed adjustment nozzle (806) is provided inside the adjustment frame (804). The top of the adjustment nozzle (806) is connected to the mounting plate (801) and is rotatably connected to it. One side of the mounting plate (801) is connected to the hose (5).

8. The pretreatment device for LIBS detection of mouse tumor tissue and normal tissue according to claim 7, characterized in that: The interior of the adjustment frame (804) has evenly distributed grooves, and an adjustment column (805) is fixedly connected to the adjustment frame (804) in the groove. The other end of the adjustment column (805) is slidably connected to the outside of the adjustment nozzle (806).

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